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Taq DNA Polymerase

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Overview

Description

Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.

Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products).

Key Features

Key Feature

Terminal Transferase Activity: Taq DNA Polymerase has terminal transferase activity which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
High-purity: No contamination activity has been detected in standard test reactions.

Properties

QC Tests

PCR^* performance, activity, nuclease

Lane	Taq	Unit
1	Leading Brand A	0.1
2		0.25
3		0.5
4	Leading Brand B	0.1
5		0.25
6		0.5
7	GenScript	0.1
8		0.25
9		0.5

Storage

Storage

Store the product at -20°C.

^* The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.

Note

Formulation

GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.

Unit Definition

One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

10 X Reaction Buffer (with Mg²⁺)

500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl₂, 1% Triton X-100
Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.

Concentration:

Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

Applications

The applications of Taq DNA Polymerase include the following:

PCR^*
3'A-tailing of blunt ends
Primer extension
DNA sequencing

Related Products

Cat. No.	Product Name	Price
E00101	Taq DNA Polymerase (1000 U) with 10 mM dNTP Mix (0.5 ml) - 1 PK	$78.00
E00043	Green Taq DNA Polymerase - 1000 U (1000.0U/vial)	$60.00
E00008	Taq DNA Polymerase without Mg²⁺ - 1000 U (1000.0U/vial)	$60.00
D0056	dNTP mixture, 10 mM each - 0.5 ml	$25.00