Description | Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR. Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products). |
Key Features | Key Feature
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QC | QC TestsPCR* performance, activity, nuclease
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Storage | StorageStore the product at -20°C.* The PCR process is covered by US. Patents Nos. 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated. |
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Note | FormulationGenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.Unit DefinitionOne unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.10 X Reaction Buffer (with Mg2+)500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity. Concentration:Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol. |
ApplicationsThe applications of Taq DNA Polymerase include the following:
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Cat. No. | Product Name | Quantity | Price |
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E00101 |
Taq DNA Polymerase (1000 U) with 10 mM dNTP Mix (0.5 ml) - 1 PK |
$78.00 |
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E00043 |
Green Taq DNA Polymerase - 1000 U (1000.0U/vial) |
$60.00 |
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E00008 |
Taq DNA Polymerase without Mg2+ - 1000 U (1000.0U/vial) |
$60.00 |
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D0056 |
dNTP mixture, 10 mM each - 0.5 ml |
$25.00 |
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