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T7 RNA Polymerase

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter. The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5'→ 3' direction, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by T7 RNA Polymerase is suitable for many downstream applications. GenScript is offering T7 RNA Polymerase produced by expression in an E. coli strain carrying a plasmid encoding the T7 RNA Polymerase.
$65.00
E00066-5

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Product Introduction
Source Recombinant T7 RNA Polymerase expressed by E.coli
Species phage T7
Unit Definition One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.
Molecular Weight ~100 kDa
Optimal active temperature 37 °C
Storage Buffer This enzyme is supplied in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 10 mM DTT, 50% Glycerol, 0.1% Triton X-100, pH8.0
Application ● Synthesis of the single-strand RNA
● Synthesis of highly labeled RNA probes
● Synthesis of precursors of siRNA
● Synthesis of precursors for RNA splicing reactions
● Synthesis of capped mRNA when a cap analog is used as a primer
Storage & Stability This product remains stable for 12 months at -20 °C. Avoid repeated freeze-thaw cycles.
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Quality Control Specifications
Appearance Clear, colorless liquid
Purity ≥ 95% as analyzed by SDS-PAGE
Enzyme Activity ≥ 50 U/μl
Endotoxin Level ≤ 0.1 EU/µg of protein as analyzed by gel clotting method
Residual Endonuclease Non-detectable
Residual Exonuclease Non-detectable
Residual RNase Non-detectable
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T7 RNA Polymerase

In vitro transcription of RNA with the recommended reaction conditions, the transcribed RNA can be up to 10 kb. »

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Background
Target Background Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter. The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5'→ 3' direction, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by T7 RNA Polymerase is suitable for many downstream applications.
GenScript is offering T7 RNA Polymerase produced by expression in an E. coli strain carrying a plasmid encoding the T7 RNA Polymerase.
References 1. J Kochetkov, S. N., E. E. Rusakova, and V. L. Tunitskaya. "Recent studies of T7 RNA polymerase mechanism." FEBS letters 440.3 (1998): 264-267.
2. Sousa, Rui, Debabrata Patra, and Eileen M. Lafer. "Model for the mechanism of bacteriophage T7 RNAP transcription initiation and termination." Journal of molecular biology 224.2 (1992): 319-334.
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


Citations
Jiaqi Wang, et al. Enhancement of CRISPR-Cas12a system through universal circular RNA design. Cell Reports Methods. (2025-06)
Wei Zhang , et al. Direct repeat region 3’ end modifications regulate Cas12a activity and expand its applications. Nucleic Acids Res. (2025-01)