Catalog Products » Inorganic Pyrophosphatase (yeast)
Inorganic Pyrophosphatase (yeast)

Lane 1: IVT RNA
Lane 2: IVT RNA + 0.05 U/ml Inorganic Pyrophosphatase

GenScript’s Inorganic Pyrophosphatase greatly improves the RNA yield by in vitro transcription.

Inorganic Pyrophosphatase (yeast)

This product is going to be discontinued.
Inorganic Pyrophosphatase (PPase) is an enzyme that catalyzes the conversion of one ion of pyrophosphate to two phosphate ions and works well in any reaction system containing Mg2+, such as the RNA in vitro transcription reaction. This enzyme can be used for the enhancement of DNA replication and improving the RNA yield by in vitro transcription. GenScript is offering Inorganic Pyrophosphatase produced by expression in an E.coli strain carrying a plasmid encoding the Inorganic Pyrophosphatase.
E00071
$60.00

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Description

Inorganic Pyrophosphatase (PPase) is an enzyme used in protein, RNA, and DNA synthesis. PPase catalyzes the conversion of one ion of pyrophosphate to two phosphate ions and works well in any reaction system containing Mg2+, such as the RNA in vitro transcription reaction. This enzyme can be used for the enhancement of DNA replication and improving the RNA yield by in vitro transcription.
GenScript is offering Inorganic Pyrophosphatase produced by expression in an E.coli strain carrying a plasmid encoding the Inorganic Pyrophosphatase. 
Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Properties
Source Recombinant Inorganic Pyrophosphatase expressed by E.coli
Species Saccharomyces cerevisiae
Molecular Weight ~33.3 kDa
Unit Definition One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 15 minute reaction at 25 °C in 100 mM Tris-HCl, pH 8.0, 3 mM MgCl2 and 10 mM PPi).
Optimal active temperature 37 °C.
Formulation Supplied as a solution of 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0 at 25 °C. 
Storage & Stability This product remains stable for up to 12 months at -20 °C. Avoid repeated freeze-thaw cycles.

Applications
·   Improve RNA yield by in vitro transcription
·   Enhancement of DNA polymerization reactions
·   Removal of contaminant PPi in reagents used for SNP genotyping by methods based on the detection of pyrophosphate

Quality Control Specifications
Appearance Clear, colorless liquid
Purity ≥ 95% as analyzed by SDS-PAGE
Enzyme Activity ≥ 0.1 U/μl
Endotoxin Level ≤ 0.1 EU/µg of protein as analyzed by gel clotting method
Residual Endonuclease Non-detectable
Residual Exonuclease Non-detectable
Residual RNase Non-detectable

Examples
  • Inorganic Pyrophosphatase (yeast)
    • Inorganic Pyrophosphatase (yeast)

    Lane 1: IVT RNA
    Lane 2: IVT RNA + 0.05 U/ml Inorganic Pyrophosphatase

    GenScript’s Inorganic Pyrophosphatase greatly improves the RNA yield by in vitro transcription.


Background
References 1.Harold, Franklin M. "Inorganic polyphosphates in biology: structure, metabolism, and function." Bacteriological Reviews 30.4 (1966): 772-794.
2.Cooperman, Barry S. "[28] The mechanism of action of yeast inorganic pyrophosphatase." Methods in enzymology 87 (1982): 526-548.

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