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phi29 DNA Polymerase

Phi29 DNA Polymerase, sourced from the Bacillus subtilis phage Phi29, is a trusted enzyme in molecular biology, particularly for cell-free plasmid amplification. GenScript's Phi29 DNA Polymerase stands out for its exceptional high fidelity, making it an ideal choice for applications such as Rolling Circle Amplification (RCA) and Whole Genome Amplification (WGA).
$45.00
E00133-250

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Product Introduction
Product Introduction phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for efficient isothermal DNA amplification. Produced from E. coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses 3'–5' proofreading exonuclease activity that acts preferentially on single-stranded DNA. Animal-origin-free (AOF) and beta-lactam-free manufacturing processes and materials are used for the enzyme manufacturing.
The enzyme was developed for preparation of DNA template used in the in vitro transcription reaction to produce nucleic acid therapeutics such as mRNA.
Source E.coli
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Species Bacillus subtilis phage phi29
Biological Activity 10 U/μL
Unit Definition The amount of enzyme required to produce 0.6 μg of RCA product from 120 ng of pET-QC template at 30 °C in 4 hours.
Theoretical Molecular Weight ~67.8 kDa, on SDS-PAGE under reducing conditions.
Concentration 0.10mg/mL±20%
Application The enzyme was developed for preparation of DNA template used in the in vitro transcription reaction to produce nucleic acid therapeutics such as mRNA.
Storage & Stability This product remains stable for 12 months at -20°C from the date of manufacture. Avoid repeated freeze-thaw cycles.
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Quality Control Specifications
Appearance Clear, colorless, liquid
Purity ≥ 98% as analyzed by SDS-PAGE
Endotoxin Level < 0.01 EU/μL of protein by gel clotting method
Residual HCD ≤0.03 ng/mL
Residual RNase Non-detectable
Residual DNase Non-detectable
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.