Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of GenScript Cas9 is equivalent to that of leading competitors.

Figure 2. The purity of GenScript Cas9 detected by SEC-HPLC is greater than 95%.

Figure 3. The purity of Cas9 Nuclease detected by RP-HPLC was higher than 95%

Figure 4. In vitro cleavage assays showed that the in vitro cleavage activity of Cas9 Nuclease was consistent across three batches, which are all higher than 85%

Figure 5. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show GenScript Cas9 has the same knockout efficiency as leading competitors.

Figure 6. Cas9 Nuclease is used for gene knockout in 293T and Jurkat cell lines. As shown in the figure, the gene knockout efficiency of the three batches of Kactus Cas9 Nuclease is comparable.