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PEmax RNase deletion

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PEmax RNase deletion involves modifications to the PEmax enzyme aimed at eliminating unwanted RNase activity, thus enhancing its stability and specificity. This advancement allows for more efficient target editing by minimizing the degradation of RNA components involved in the editing process, further improving the overall performance of the prime editing protocol.
RC00003
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Overview
Description PEmax RNase deletion involves modifications to the PEmax enzyme aimed at eliminating unwanted RNase activity, thus enhancing its stability and specificity. This advancement allows for more efficient target editing by minimizing the degradation of RNA components involved in the editing process, further improving the overall performance of the prime editing protocol.
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Applications
single guide RNA (sgRNA) dependent single-stranded DNA cleavage
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Properties
Expression System Recombinant Cas9-based fusion with three NLS expressed by E.coli
Species S. pyogenes
PAM NGG
Tag C-His tag
Molecular Weight 223.714KDa
Storage & Stability This product is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C.
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Quality Control Specifications
Purity ≥90% by SDS-PAGE, 98% by SEC-HPLC
Residual DNase Non-specific DNase activity
Endotoxin Level Control <10EU/mg
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PEmax RNase Deletion

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PEmax RNase Deletion

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Background
References David R Liu,et al.“Phage-assisted evolution and protein engineering yield compact, efficient prime editors”. Cell. 2023 Aug 31;186(18):3983-4002.e26.
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