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PEmax

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PEmax is an improved version of PE2 with additional NLS and improved nCas9 domain, facilitating precise genome editing without the need for double-stranded DNA breaks. It significantly increases editing efficiency and accuracy compared to earlier systems and allows for a broader range of base conversions and edits within the genome design
RC00005
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Overview
Description PEmax is an improved version of PE2 with additional NLS and improved nCas9 domain, facilitating precise genome editing without the need for double-stranded DNA breaks. It significantly increases editing efficiency and accuracy compared to earlier systems and allows for a broader range of base conversions and edits within the genome design
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Applications
single guide RNA (sgRNA) dependent single-stranded DNA cleavage
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Properties
Expression System Recombinant Cas9-based fusion with three NLS expressed by E.coli
Species S. pyogenes
PAM NGG
Tag C-His tag
Molecular Weight 243.359KDa
Storage & Stability PEmax is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C.
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Quality Control Specifications
Purity ≥90% by SDS-PAGE, 86% by SEC-HPLC
Residual DNase Non-specific DNase activity
Endotoxin Level Control <10EU/mg
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PEmax

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PEmax

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Background
References 1. David R Liu, et al. “Enhanced prime editing systems by manipulating cellular determinants of editing outcomes”. Cell. 2021 Oct 28;184(22):5635-5652.e29. 2. David R Liu, et al. “Phage-assisted evolution and protein engineering yield compact, efficient prime editors”. Cell. 2023 Aug 31;186(18):3983-4002.e26.
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