Overview

A mouse monoclonal antibody is a type of antibody produced by a single clone of immune cells derived from mice. The specificity and affinity of mouse monoclonal antibodies make them a valuable resource for a wide range of applications, such as research and development (R&D), diagnostics, and therapeutics. They are valuable tools for identifying and quantifying proteins, detecting pathogens and toxins, and treating various diseases, including cancer and autoimmune disorders.

GenScript's custom mouse monoclonal antibody development service provides a comprehensive solution for generating high-quality monoclonal antibodies against any target of interest. The service includes immunogen design and synthesis, immunization, hybridoma generation, screening, and antibody production. Our team of technical account managers, all holding PhDs, will collaborate with you to design a custom protocol that meets your unique requirements. With GenScript's fully customizable monoclonal antibody generation services, you have complete control over every stage of the process, allowing you to modify your approach, restart a phase, or terminate the project if necessary. Avoid the risks associated with a rigid protocol and choose our fully customizable service that provides the ultimate flexibility. Partnering with GenScript's years of expertise in custom antibody development can help you achieve success.

Highlights

Industry-Leading Antibody Delivery

Industry-Leading Antibody Delivery

  • Deliver purified antibodies as fast as 11 wks
  • Guaranteed deliverables with competitive price
  • High success rate for challenging projects
Cutting-Edge  Technology & Facility

Cutting-Edge Technology & Facility

  • ImmunoPlus™: break the immune tolerance
  • MonoExpress™: fast mouse immunization
  • AAALAC and OLAW certificated animal facility
Various Antibody Production Technologies

Various Antibody Production Technologies

  • High-yield roller bottle antibody expression
  • Serum-free recombinant antibody expression
  • Ascites production for in-vivo antibody scale-up

Service Details

Custom Mouse mAb Development Package 1
Standard Mouse mAb Development Package 2
TurboMab Mouse mAb Development PackageNew 3

Custom Mouse mAb Development Package

Custom Mouse mAb Development Package
Custom Mouse Monoclonal Antibody Package TAT Deliverables
Phase I (Optional)
Immunogen Preparation
  • Customer-provided protein/peptide antigen
  • GenScript generated protein/peptide: antigen synthesis and conjugation
  • Other antigen type*
  • ImmunoPlus™: to break the immune tolerance (Homology > 90%, optional)
  • Customer-provided: starts from 1 wk
  • Peptide synthesis: starts from 2 wks
  • Deliver remaining antigen (peptide or protein) when the project is finished
Phase II
Animal Immunization
  • Immunization options:
    • Option 1: Conventional immunization protocol
    • Option 2: MonoExpress™ fast immunization protocol
  • Conventional: starts from 6 wks
  • MonoExpress™: ~2 wks
  • Deliver post-immune test samples for conventional immunization
Phase III
Cell Fusion & Screening
  • One cell fusion with up to 15 plates
  • Counter screening if required
  • 4~6 wks (including the final boost)
  • Supernatants from up to 10~20 parental clones, 2 ml/clone
  • CoA report
Option 1: Hybridoma Cell Expression Option 2: Recombinant Expression
Phase IV
Subcloning, Expansion and Cryopreservation
  • Two rounds of subcloning
  • 2 vials of each subclonal hybridoma cell line from up to 5 positive primary cell lines
  • One round of subcloning from up to 5 positive primary cell lines
  • Antibody sequencing
  • Option 1: 6~8 wks
  • Option 2: 3~5 wks
  • Option 1: 5 ml supernatant of each subclonal cell line
  • Option 2: Antibody sequencing report
Phase V
Ab Production (Optional)
  • Antibody production by roller bottle
  • Recombination antibody production with CHO cell
  • Option 1: ~4 wks
  • Option 2: 1~2 wks
  • 1-5 mg purified mAb/clone
Total TAT Starts from 17 wks Starts from 11 wks - -

*Immunogen type: protein, peptides, small molecules, DNA, mRNA, virus-like particles, cell lines and etc.

Standard Mouse mAb Development Package

Standard Mouse mAb Development Package
Standard Mouse Monoclonal Antibody Package TAT Deliverables
Phase I (Optional)
Immunogen Preparation*
  • Protein: Customer provides/GenScript synthesizes
  • Peptide: GenScript synthesized only (Free of charge)
  • Customer provide: starts from 1 wk
  • Peptide synthesis: 1~2 wks
  • Deliver the remaining antigen (2mg peptide) when the project is finished
Phase II
Animal Immunization, Cell Fusion and Screening
  • Immunization Protocol Options: Option 1: Conventional; Option 2: MonoExpress™
no post-immunization test samples delivered for either option
  • Option 1: Conventional: 9~12 wks
  • Option 2: MonoExpress™: 5~7 wks
  • 20 ELISA (+) Supernatants, 2 ml/clone (Guaranteed)
  • CoA report
Phase III
Subcloning,Expansion,Cryopreservation and Antibody production
  • Small-scale production after subcloning
  • 5 positive clones delivered
  • Additional clones with extra charge
  • 7~10 wks
  • 5 subclonal hybridoma cell lines (2 vials/cell line) (Guaranteed)
  • 5ml supernatant of each subclonal cell
  • 1 clone purified mAb (0.1 mg) ELISA > 1:64,000 (Guaranteed)
  • CoA
Total TAT - Starts from 13 wks

*:
a) Immunogen type: protein or peptide
b) Immunogen requirement for customer-provided protein: Purity ≥ 75%; Quantity: ≥ 1.5, Concentration ≥ 0.4mg/ml; Homology ≤ 90%; MW >10KD; no organic solvents in buffer; His/Flag tag protein

TurboMab Mouse mAb Development Package

Standard Mouse mAb Development Package
TurboMab platform TAT Deliverables
Phase I
Immunogen preparation
(Optional)
  • Customer-provided protein/peptide antigen
  • GenScript generated protein/peptide: antigen synthesis and conjugation
  • Other antigen type*
  • ImmunoPlus™: to break the immune tolerance (Homology > 90%, optional)
1~2 /
Phase II
Animal Immunization
  • Immunization options:
    • Option 1: Conventional immunization protocol
    • Option 2: MonoExpress™ fast immunization protocol
  • Conventional: starts from 6 wks
  • MonoExpress™: ~2 wks
  • Deliver post-immune test samples for conventional immunization
Silver Diamond
Phase III
Positive B cell enrichment, cell fusion & screening
  • Plasma cell enrichment and cell fusion up to 10 plates
  • Plasma cells enrichment and cell fusion with up to 30 plates
  • 1~2 (Report only)
  • 2~3 (Report and supernatant)
  • Silver: Reports for up to 20 immunogen positive clones or with immunogen positive supernatant clones (2ml/clone)
  • Diamond: Reports for up to 100 immunogen positive clones or with 20 immunogen positive supernatant clones (2ml/clone)
Counter screening if required / /
Phase IV
Sequencing & Antibody production
  • Option 1: Antibody sequencing and validation for up to 5 clones
/ 3~4
  • Antibody sequencing reports and up to 5 purified Ab (for silver package only)
  • Option 2: Antibody sequencing for variable region (NGS) + Gene synthesis & recombinant supernatant
3~5
  • Antibody sequencing reports & 0.5ml recombinant supernatant/clone (for silver or diamond package)
Phase V
Ab Production
(Optional)
  • Recombinant Ab production & Ab purification
2~3
  • Purified Abs, recombination positive rate not guaranteed
Total Starts from 8 wks -

*Immunogen type: protein, peptides, small molecules, DNA, mRNA, virus-like particles, cell lines and etc.

Case Studies

Related Resources

White Paper

Antibody Generation at its Best

Learn why hybridoma technology is preferred for high quality antibody generation, and see how it compares to other antibody generation methods!

Webinar

Anti-Idiotypic Antibody Platforms for Accelerating Antibody Drug Discovery

In this webinar you will learn about:

1. Overcoming timeline challenges in ADD with GenScript’s anti-ID ab platforms

2. Our capabilities, illustrated through many success case studies in generating antibody drug conjugates, scFv, VHH, bi-specific antibody and etc.

3. Strategies and advantages of ADA and PK ELISA kits development

Overcoming Timeline Challenges In Antibody Drug Discovery

In this webinar you will learn about:

1. Antibody-drug discovery and development

2. Lead optimization strategies in antibody drug development

3. Overcoming timeline challenges using GenScript’s antibody discovery and expression platforms

Additional Services

Get in Touch
with GenScript Custom
Antibody Services

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Related Services

Overwhelmed by options? Using our Semi-custom Monoclonal Antibody Production Services, you can go from antigen sequence to antibody production with as little as 3 easy decisions. Searching for our monoclonal antibodies guaranteed to work in your system? Look no further than our MonoExpress™ Premium Service. GenScript also offers industry leading comprehensive guaranteed packages for monoclonal antibody development including:

Download our service selection guide to help decide which package best fits your needs.

If you would like to provide your own antigen, please fill out the Antigen Submission Form and mail it together with your antigen to:

  • Antibody Services, GenScript, USA.
  • 860 Centennial Ave.
  • Piscataway, NJ 08854, US.

Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.

Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

Fill out the online form and email to [email protected]
A Technical Account Manager will reply you within 24 hours to finalize the quote
To place order, provide credit card/PO information
Production of your project begins immediately
A Project Manager is in contact with you during the production process to give updates

REQUEST A QUOTE

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