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Gene Synthesis & Molecular Biology Services
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Highly Customizable Cloning & Subcloning Services

Save Time With Expression-ready

100% Sequence Verified Gene Constructs

150+ IP Free Vectors Provided

GenScript offers cloning services so you can free yourself from routine gene cloning. Our molecular biology experts can bundle gene synthesis with cloning into your choice of vector, or you can outsource DNA cloning projects from templates you already have. For your convenience, gene cloning can easily be added onto your gene synthesis order when you  request a quote online.

Cloning and Subcloning Service Bundles

SERVICES Express Cloning SC1691
Bundled with gene synthesis
VectorArk™ Cloning SC1692
Bundled with gene synthesis
Subcloning SC1017
Bundled with gene synthesis
Advantages IP Free Vectors – Choose from over 150 popular vectors listed below Save on cloning into your archived vectors Get ready to use clones in any vector, commercial or customized
What you provide
  • Electronic sequence for gene synthesis
  • Vector name
  • Specify cloning sites if desired
  • Electronic sequence for gene synthesis
  • Name of your previously archived vector
  • Specify cloning sites if desired
  • Electronic sequence for gene synthesis
  • Vector details (map) & cloning strategy
  • Mail in an aliquot of your vector
What you receive
  • 4 μg (1 μg for low-copy plasmid) lyophilized plasmid DNA of your insert in your chosen vector
  • Sequence chromatograms, plasmid construct map, and QC data
  • 4 μg (1 μg for low-copy plasmid) lyophilized plasmid DNA of your insert in a standard vector PLUS
  • 4 μg (1 μg for low-copy plasmid) lyophilized plasmid DNA of your insert in your chosen vector
  • Sequence chromatograms, plasmid construct map, and QC data
Starting price $49* $49 $149*
Starting turnaround time 2 days* 5 days 5 days*

*Price and turnaround time listed here are in addition to the price and turnaround time for gene synthesis

custom PCR cloning services

Custom PCR Cloning Services

Template synthesized at GenScript

SC1625 Starting at $149 | 10 business days

Ship your own template to GenScript

SC1622 Starting at $295 | 14 business days

Quote/Order Request Now

Vector Selector

GenScript has the largest selection of IP FREE vectors in industry, providing you with highly customizable solutions.

Find the right expression vector for you by considering the expression host, promoter, bacterial selection, copy number, or epitope tag.

Click on the vector name to view the full vector map.

Host:

Promoter:

Bacterial Antibiotics:

Copy Number:

N-terminal Epitope:

Cleavage Site:

C-terminal Epitope:


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Vector Name and Map Host Promoter Bacterial Antibiotics Copy number N-terminal Epitope tag Cleavage site C-terminal Epitope tag
pGenLenti Mammalian T7, CMV Ampicillin High C-DYKDDDDK
pcDNA3.1(+) Mammalian CMV Ampicillin High
pcDNA3.1(-) Mammalian CMV Ampicillin High
pcDNA3.1(+)-myc-His A Mammalian CMV Ampicillin High C-Myc, C-His
pcDNA3.1(+)-myc-His B Mammalian CMV Ampicillin High C-Myc, C-His
pcDNA3.1(+)-myc-His C Mammalian CMV Ampicillin High C-Myc, C-His
pcDNA3.1(-)-myc-His A Mammalian CMV Ampicillin High C-Myc, C-His
pcDNA3.1(-)-myc-His B Mammalian CMV Ampicillin High C-Myc, C-His
pcDNA3.1(-)-myc-His C Mammalian CMV Ampicillin High C-Myc, C-His
pCI-Neo Mammalian CMV Ampicillin High
pcDNA3.1(+)-C-DYK Mammalian CMV Ampicillin High C-DYKDDDDK
pcDNA3.1(+)-C-HA Mammalian CMV Ampicillin High C-HA
pcDNA3.1(+)-C-6His Mammalian CMV Ampicillin High C-His
pcDNA3.1(+)-C-Myc Mammalian CMV Ampicillin High C-MYC
pcDNA3.1(+)-N-DYK Mammalian CMV Ampicillin High N-DYKDDDDK
pcDNA3.1(+)-N-HA Mammalian CMV Ampicillin High N-HA
pcDNA3.1(+)-N-6His Mammalian CMV Ampicillin High N-His
pcDNA3.1(+)-N-Myc Mammalian CMV Ampicillin High N-Myc
pcDNA3.1(+)-N-GST(Thrombin) Mammalian CMV Ampicillin High N-GST(Thrombin) Thrombin
pcDNA3.1(+)-N-GST(TEV) Mammalian CMV Ampicillin High N-GST(TEV) TEF
pcDNA3.1/Hygro(+) Mammalian CMV Ampicillin High
pcDNA3.1/Hygro(-) Mammalian CMV Ampicillin High
pcDNA3.1/Zeo (+) Mammalian CMV Ampicillin High
pcDNA3.1/Zeo (-) Mammalian CMV Ampicillin High
pCMV-3Tag-1a Mammalian CMV Kanamycin High N-3*DYKDDDDK
pCMV-3Tag-2a Mammalian CMV Kanamycin High N-3*Myc
pCMV-3Tag-3a Mammalian CMV Kanamycin High C-3*DYKDDDDK
pCMV-3Tag-4a Mammalian CMV Kanamycin High C-3*Myc
pcDNA3.1(+)-C-eGFP Mammalian CMV Ampicillin High C-eGFP
pcDNA3.1(+)-N-eGFP Mammalian CMV Ampicillin High N-eGFP
pcDNA3.1(+)-P2A-eGFP Mammalian CMV Ampicillin High C-eGFP 
pcDNA3.1(+)-P2A Mammalian CMV Ampicillin High
pcDNA3.1(+)-N-DYK-P2A Mammalian CMV Ampicillin High C-DYKDDDDK
pcDNA3.1(+)-C-DYK-P2A Mammalian CMV Ampicillin High C-DYKDDDDK
pCMV-3Tag-1a-P2A Mammalian CMV Kanamycin High N-3*DYKDDDDK
pCMV-3Tag-3a-P2A Mammalian CMV Kanamycin High C-3*DYKDDDDK
pcDNA3.4 Mammalian CMV Ampicillin High
pBluescript II KS(-) Bacterial T7 Ampicillin High
pBluescript II KS(+) Bacterial T7 Ampicillin High
pBluescript II SK(-) Bacterial T7 Ampicillin High
pBluescript II SK(+) Bacterial T7 Ampicillin High
pET-3a Bacterial T7 Ampicillin Low N-T7
pET-3b Bacterial T7 Ampicillin Low N-T7
pET-3c Bacterial T7 Ampicillin Low N-T7
pET-3d Bacterial T7 Ampicillin Low N-T7
pET-9a Bacterial T7 Kanamycin Low N-T7
pET-11a Bacterial T7 & Lac Ampicillin Low N-T7
pET-11b Bacterial T7 & Lac Ampicillin Low N-T7
pET-11c Bacterial T7 & Lac Ampicillin Low N-T7
pET-11d Bacterial T7 & Lac Ampicillin Low N-T7
pET-14b Bacterial T7 Ampicillin Low N-His
pET-15b Bacterial T7 & Lac Ampicillin Low N-His
pET-16b Bacterial T7 & Lac Ampicillin Low N-His
pET-17b Bacterial T7 Ampicillin Low N-T7
pET-19b Bacterial T7 & Lac Ampicillin Low N-His
pET-20b(+) Bacterial T7 Ampicillin Low N-pelB C-His
pET-21a(+) Bacterial T7 & Lac Ampicillin Low N-T7 C-His
pET-21b(+) Bacterial T7 & Lac Ampicillin Low N-T7 C-His
pET-21d(+) Bacterial T7 & Lac Ampicillin Low N-T7 C-His
pET-22b(+) Bacterial T7 & Lac Ampicillin Low N-pelB C-His
pET-23a(+) Bacterial T7 Ampicillin Low N-T7 C-His
pET-24a(+) Bacterial T7 & Lac Kanamycin Low N-T7 C-His
pET-24b(+) Bacterial T7 & Lac Kanamycin Low N-T7 C-His
pET-24c(+) Bacterial T7 & Lac Kanamycin Low N-T7 C-His
pET-24d(+) Bacterial T7 & Lac Kanamycin Low N-T7 C-His
pET-25b(+) Bacterial T7 & Lac Ampicillin Low N-pelB C-HSV, C-His
pET-26b(+) Bacterial T7 & Lac Kanamycin Low N-pelB C-His
pET-27b(+) Bacterial T7 & Lac Kanamycin Low N-pelB C-HSV, C-His
pET-28a(+) Bacterial T7 & Lac Kanamycin Low N-His, N-T7 C-His
pET-28b(+) Bacterial T7 & Lac Kanamycin Low N-His, N-T8 C-His
pET-28c(+) Bacterial T7 & Lac Kanamycin Low N-His, N-T9 C-His
pET-29a(+) Bacterial T7 & Lac Kanamycin Low N-S-tag C-His
pET-29b(+) Bacterial T7 & Lac Kanamycin Low N-S-tag C-His
pET-29c(+) Bacterial T7 & Lac Kanamycin Low N-S-tag C-His
PET-30a(+) Bacterial T7 & Lac Kanamycin Low N-His, N-S-tag C-His
PET-30b(+) Bacterial T7 & Lac Kanamycin Low N-His, N-S-tag C-His
PET-30c(+) Bacterial T7 & Lac Kanamycin Low N-His, N-S-tag C-His
PET-31b(+) Bacterial T7 & Lac Ampicillin Low N-KSI C-His
pET-32a(+) Bacterial T7 & Lac Ampicillin Low N-Trx C-His
pET-32b(+) Bacterial T7 & Lac Ampicillin Low N-Trx C-His
pET-41a(+) Bacterial T7 & Lac Kanamycin Low N-GST, N-His C-S-tag, C-His
pET-41b(+) Bacterial T7 & Lac Kanamycin Low N-GST, N-His C-S-tag, C-His
pET-41c(+) Bacterial T7 & Lac Kanamycin Low N-GST, N-His C-S-tag, C-His
pET-42a(+) Bacterial T7 & Lac Kanamycin Low N-GST, N-His C-S-tag, C-His
pET-42b(+) Bacterial T7 & Lac Kanamycin Low N-GST, N-His C-S-tag, C-His
pET-42c(+) Bacterial T7 & Lac Kanamycin Low N-GST, N-His C-S-tag, C-His
pET-43.1a(+) Bacterial T7 & Lac Ampicillin Low N-GST, N-His C-S-tag, C-His
pET-43.1b(+) Bacterial T7 & Lac Ampicillin Low N-GST, N-His C-S-tag, C-His
pET-45b(+) Bacterial T7 & Lac Ampicillin Low N-His C-S-tag
pET-50b(+) Bacterial T7 & Lac Kanamycin Low N-His, N-Nus C-S-tag
pET-51b(+) Bacterial T7 & Lac Ampicillin Low N-Strep C-His
pET-52b(+) Bacterial T7 & Lac Ampicillin Low N-Strep C-His
pET-24a(+)-TEV Bacterial T7 & Lac Kanamycin Low N-T7 TEV C-His
pET-28a(+)-TEV Bacterial T7 & Lac Kanamycin Low N-T7 TEV C-His
pGEX-2TK Bacterial Tac Ampicillin Low N-GST
pGEX-4T-1 Bacterial Tac Ampicillin Low N-GST
pGEX-4T-2 Bacterial Tac Ampicillin Low N-GST
pGEX-4T-3 Bacterial Tac Ampicillin Low N-GST
pGEX-5X-1 Bacterial Tac Ampicillin Low N-GST
pGEX-5X-2 Bacterial Tac Ampicillin Low N-GST
pGEX-5X-3 Bacterial Tac Ampicillin Low N-GST
pGEX-6P-1 Bacterial Tac Ampicillin Low N-GST
pGEX-6P-2 Bacterial Tac Ampicillin Low N-GST
pGEX-6P-3 Bacterial Tac Ampicillin Low N-GST
pMAL-c4x Bacterial Tac Ampicillin Low N-MBP
pMAL-c5E Bacterial Tac Ampicillin Low N-MBP
pMAL-c5x Bacterial Tac Ampicillin Low N-MBP
pMAL-p5E Bacterial Tac Ampicillin Low N-MBP
pMAl-p5g Bacterial Tac Ampicillin Low N-MBP
pMAl-p5x Bacterial Tac Ampicillin Low N-MBP
pQE-1 Bacterial T5,lac Ampicillin Low N-His
pQE-60 Bacterial T5,lac Ampicillin Low C-His
pGS-21a Bacterial T7,lac Ampicillin Low N-His, N-GST
pETDuet-1 Bacterial T7,lac Ampicillin Low N-His
pCDFDuet-1 Bacterial T7,lac Streptomycin Low N-His
pRSFDuet-1 Bacterial T7,lac Kanamycin Low N-His
pCOLADuet-1 Bacterial T7,lac Kanamycin Low N-His
pGEX-4T-1-H(RBS) Bacterial Tac Ampicillin Low N-GST
pGEX-4T-1-M(RBS) Bacterial Tac Ampicillin Low N-GST
pGEX-5X-1-H(RBS) Bacterial Tac Ampicillin Low N-GST
pGEX-5X-1-M(RBS) Bacterial Tac Ampicillin Low N-GST
pGEX-6P-1-H(RBS) Bacterial Tac Ampicillin Low N-GST
pGEX-6P-1-M(RBS) Bacterial Tac Ampicillin Low N-GST
pMAL-c4x-1-H(RBS) Bacterial Tac Ampicillin Low N-MBP
pMAL-c4x-1-M(RBS) Bacterial Tac Ampicillin Low N-MBP
pBacPAK8 Baculovirus polyhedrin Ampicillin High
pBacPAK9 Baculovirus polyhedrin Ampicillin High
pAcG2T Baculovirus polyhedrin Ampicillin High N-GST
pAcHLT A Baculovirus polyhedrin Ampicillin High N-His
pAcHLT B Baculovirus polyhedrin Ampicillin High N-His
pAcHLT C Baculovirus polyhedrin Ampicillin High N-His
pAcGHLT A Baculovirus polyhedrin Ampicillin High N-His
pAcGHLT B Baculovirus polyhedrin Ampicillin High N-His
pAcGHLT C Baculovirus polyhedrin Ampicillin High N-His
pAcSG2 Baculovirus polyhedrin Ampicillin High
pBAC-1 Baculovirus polyhedrin Ampicillin High C-His
pFastBac1 Baculovirus polyhedrin Ampicillin High
pFastBacHT-A Baculovirus polyhedrin Ampicillin High N-His TEV
pFastBacHT-B Baculovirus polyhedrin Ampicillin High N-His TEV
pFastBacHT-C Baculovirus polyhedrin Ampicillin High N-His TEV
pFastBac-Dual Baculovirus polyhedrin, p10 Ampicillin High
pAO815 Pichia AOX1 Ampicillin High
pPIC 3.5k Pichia AOX1 Ampicillin High
pPIC9 Pichia AOX1 Ampicillin High N-α-factor secretion signal
pPIC9K Pichia AOX1 Ampicillin High
pPICZA Pichia AOX1 Zeocin High C-Myc, C-His
pPICZB Pichia AOX1 Zeocin High C-Myc, C-His
pPICZC Pichia AOX1 Zeocin High C-Myc, C-His
pPICZalphaA Pichia AOX1 Zeocin High N-α-factor secretion signal C-Myc, C-His
pPICZalphaB Pichia AOX1 Zeocin High N-α-factor secretion signal C-Myc, C-His
pPICZalphaC Pichia AOX1 Zeocin High N-α-factor secretion signal C-Myc, C-His
pESC-TRP S. cerevisiae GAL1, GAL10 Ampicillin High C-DYKDDDDK, C-Myc
pESC-URA S. cerevisiae GAL1, GAL10 Ampicillin High C-DYKDDDDK, C-Myc
pESC-HIS S. cerevisiae GAL1, GAL10 Ampicillin High C-DYKDDDDK, C-Myc
pESC-LEU S. cerevisiae GAL1, GAL10 Ampicillin High C-DYKDDDDK, C-Myc

Testimonials

We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!

Dr. Melissa A. Melan,
University of Pittsburgh Medical Center, USA

Delivery Specifications

All of our Custom Cloning services guarantee 100% sequence fidelity and deliver everything you need to put your new constructs to work right away. Our delivery package includes the following:

  • 4 μg of lyophilized plasmid containing your gene insert (1 μg for low-copy plasmid) *
  • Sequence chromatograms covering your gene (electronic)
  • Construct map for the plasmid (electronic)
  • Quality assurance certificate

*Check plasmid copy

For cloning projects involving customer-provided templates, please send your samples with full shipping information and PO number (if applicable) with a hard copy of the completed PCR Cloning and Subcloning Quotation Request Form to PCR Cloning and Subcloning Services, GenScript Inc., 860 Centennial Avenue., Piscataway, NJ 08854, U.S.