PTCH2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005629080.2	XP_005629137.1	protein patched homolog 2 isoform X1
XM_005629080.3	XP_005629137.1	protein patched homolog 2 isoform X1
XM_005629082.3	XP_005629139.1	protein patched homolog 2 isoform X7
XM_014119704.1	XP_013975179.1	protein patched homolog 2 isoform X2
XM_539636.7	XP_539636.3	protein patched homolog 2 isoform X3
XM_022428023.1	XP_022283731.1	protein patched homolog 2 isoform X4
XM_005629082.2	XP_005629139.1	protein patched homolog 2 isoform X5
XM_014119704.2	XP_013975179.1	protein patched homolog 2 isoform X2
XM_005629081.3	XP_005629138.1	protein patched homolog 2 isoform X5
XM_539636.6	XP_539636.3	protein patched homolog 2 isoform X3
XM_005629081.2	XP_005629138.1	protein patched homolog 2 isoform X4
XM_022428025.1	XP_022283733.1	protein patched homolog 2 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe17389	XM_005629082.3 Latest version!	Canis lupus familiaris patched 2 (PTCH2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe293508	XM_022428023.1 Latest version!	Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe293509	XM_022428025.1 Latest version!	Canis lupus familiaris patched 2 (PTCH2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe40354	XM_014119704.2 Latest version!	Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe17386	XM_005629080.3 Latest version!	Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe17387	XM_539636.7 Latest version!	Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe17388	XM_005629081.3 Latest version!	Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe17386	XM_005629080.2	Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe17389	XM_005629082.2	Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe40354	XM_014119704.1	Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe17387	XM_539636.6	Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe17388	XM_005629081.2	Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

PTCH2 ( XM_005629082.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629139.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe17389
Clone ID Related Accession (Same CDS sequence)	XM_005629082.3 , XM_005629082.2
Accession Version	XM_005629082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein patched homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT 
GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT 
CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT 
CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629139.1
CDS	121..3117
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRISGPAALLPAGYLHPAGMHFPRLCPAAAQPLDGWPHRTGPGDDDCGALWHHGFPGHQTECHPRGDPCGLCGHWC

Target ORF information:

RefSeq Version	XM_005629082.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629082.3

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT 
GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT 
CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT 
CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005629082.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629139.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe17389
Clone ID Related Accession (Same CDS sequence)	XM_005629082.3 , XM_005629082.2
Accession Version	XM_005629082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein patched homolog 2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT 
GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT 
CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT 
CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629139.1
CDS	363..3359
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRISGPAALLPAGYLHPAGMHFPRLCPAAAQPLDGWPHRTGPGDDDCGALWHHGFPGHQTECHPRGDPCGLCGHWC

Target ORF information:

RefSeq Version	XM_005629082.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629082.2

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATATCTGG GCCTGCGGCG CTACTTCCTG CTGGCTATCT GCATCCTGCT 
GGTATGCACT TTCCTCGTCT GTGCCCTGCT GCTGCTCAAC CCCTGGACGG CTGGCCTCAT 
CGTACTGGTC CTGGCGATGA TGACTGTGGA GCTCTTTGGC ATCATGGGTT TCCTGGGCAT 
CAAACTGAGT GCCATCCCCG TGGTGATCCT TGTGGCCTCT GTGGGCATTG GTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_022428023.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283731.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.

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CloneID	OCe293508
Clone ID Related Accession (Same CDS sequence)	XM_022428023.1
Accession Version	XM_022428023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein patched homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGCGCTTCA 
GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC TGGCTACACT 
ATTACCGGAA CTGGCTCCTG GGTGAGAGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT 
TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC 
AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA 
CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC 
ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA 
CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG 
GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG 
GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG 
GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG 
CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG 
CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG 
GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC 
CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG 
ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA 
GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT 
GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC 
CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA 
CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC 
AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG 
GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC 
ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA 
CCATGTCCAG CCACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283731.1
CDS	122..3742
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLSASALSRPCCPHLPPRRRAPGYTITGTGSWVRGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_022428023.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022428023.1

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGCGCTTCA 
GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC TGGCTACACT 
ATTACCGGAA CTGGCTCCTG GGTGAGAGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT 
TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC 
AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA 
CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC 
ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA 
CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG 
GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG 
GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG 
GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG 
CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG 
CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG 
GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC 
CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG 
ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA 
GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT 
GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC 
CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA 
CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC 
AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG 
GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC 
ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA 
CCATGTCCAG CCACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_022428025.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283733.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe293509
Clone ID Related Accession (Same CDS sequence)	XM_022428025.1
Accession Version	XM_022428025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein patched homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAC AGACCCCACG CCAGGAAGGG GAGAACGTCC TCACCCCCGA GGCGCTCGGC 
CTCCACCTCC AGGCAGCCCT CACCGCCAGT AAAGTGCAAG TATCACTCTA CGGAAAGTCC 
TGGGATTTGA ACAAAATCTG CTACAAGTCA GGGATTCCCC TAATTGAAAA TGGAATGATT 
GAGCGGATGA TAGAGAAGCT GTTTCCGTGC GTGATCCTCA CCCCCCTCGA CTGCTTCTGG 
GAGGGCGCCA AGCTCCAAGG GGGCTCAGCC TACTTGCCTG GCCGCCCTGA CATCCAGTGG 
ACCAACCTGG ATCCAGAGCA GCTGCTGGAG GAGCTGGGGC CCTTTGCCTC CCTCGAGGGC 
TTCCGGGAGC TGCTAGACAA GGCACAGGTG GGCCAGGCCT ATGTGGGGCG GCCCTGTCTG 
CACCCTGACG ACCTCCACTG CCCGCCTAGT GCCCCTAACC ATCACAGCAA GCAGGCTCCC 
AACGTGGCTC AGGAGTTGAG TGGGGGCTGT CACGGCTTTT CCCACAAGTT CATGCACTGG 
CAGGAGGAAC TGCTGCTGGG GGGCATGGCC AGAGACCCCC AAGGACAGCT GCTGAGGGCG 
GAGGCCCTGC AGAGCACCTT CCTGCTGATG AGTCCCCGCC AGCTGTACGA GCACTTCCGG 
GGCGACTACC AGACGCATGA CATCGGCTGG AGCGAGGAGC AGGCGGGGAC AGTGCTACAG 
GCCTGGCAGC GGCGCTTCGT GCAGGTCAGT GTGCGGATGG GCAAGGGCGG TGCCCGAGGC 
TGCTCCCTCC TCCTGCCCCT CAGATCCACC CTGTCTCTGC AGCTGGCTCA GGAGGCCCTG 
CCTCAGAATT CATCCCAGCA GATCCATGCC TTTTCCTCCA CCACTCTGGA TGACATCCTG 
CATGCTTTCT CTGAAGTCAG TGCTGCCCGT GTGGTCGGAG GCTATCTGCT CATGCTAGCC 
TATGCTTGCG TGACGATGCT GCGCTGGGAC TGTGCCCAGT CCCAGGGTGC CGTGGGCCTT 
GCGGGGGTGC TGCTGGTAGC CCTGGCGGTG GCCTCGGGCC TTGGGCTCTG CGCCCTGCTG 
GGCATCGCCT TCAATGCCGC CACTACCCAG GTGCTGCCCT TCTTGGCACT GGGCATCGGC 
GTGGATGACA TATTCCTGCT GGCACATGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC 
CAGGAGCGCA CAGGTGAGTG TCTGCAGCGC ACGGGCACCA GCGTCGCACT CACGTCCATC 
AGCCACATGG TTGCCTTCTT CATGGCCGCC CTGGTTCCCA TCCCGGCCCT GCGGGCCTTC 
TCCCTGCAGG CAGCCATAGT CGTTGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GTCTGGACCT GCACCGACGC CACTGCCAGC GCCTTGACGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC GCGGGTAATT CAGATCCTGC CCCAGGAACT GGGGGACGGG 
ACGGTGCCAG TGGGCATCGC CCACCTGACC GCCACGGTTC AAGCCTTTGC CCACTGCGAA 
GCCGGCAGCC AGCACGTCGT CACCATCCTG CCTCCCCGAG CCCGCTTGGT GCCCCCACCT 
TCTGACCCAC TGGGCTCTGA GCTCTTCAGC CCAGGAGGAT CCACACGGGA CCTTCTAGGC 
CAAGAGGAGG GGACGAGACA AAAGGCAACC TGCAGCTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TTGCCCGCTC TCAGTTTGCA CCCTTGCTGC TCCAGTCACA CAGCAAGGCC 
ATCGTGCTGG TGCTCTTTGG GGCTCTCCTG GGCCTGAGCC TCTACGGAGC AACCTTGGTG 
CAGGATGGGC TGGCCCTGAC GGATGTGGTG CCTCGGGGCA CCAAGGAGCA CGCCTTCCTG 
AGCGCCCAGC TCAGGTACTT CTCCCTGTAC GAGGTGGCCC TGGTGACGCA GGGTGGCTTT 
GACTACGCCC ACTCCCAACG CGCCCTCTTT GATCTGCACC AGCGCTTCAG CTCTCTCAAG 
GCCGTGCTGC CCACACCTGC CACCCAGGCG CCGCGCACCT GGCTACACTA TTACCGGAAC 
TGGCTCCTGG GAATCCAGGC CGCCTTTGAC CAGGACTGGG CTTCTGGGCG CATTAGCCGC 
CACTCGTGCC GCAATGGCTC CGAGGACGGG GCCCTGGCCT ACAAGCTGCT CCTCCAGACC 
GGGGATGCCC AAGAGCCTCT GGATTTCAGC CAGCTGACCA CACGGAAGCT GGTAGACAAG 
GAGGGGCTGA TTGCACCTGA GCTCTTCTAC GTGGGGCTGA CCATGTGGGT AAGCAGTGAC 
CCGCTGGGCC TGGCGGCCTC ACAGGCCAAC TTCTACCCCC CACCTCCCGA GTGGCTGCAC 
GACAAGTACG ACACCACCGG GGAGAACCTT CGCATCCCGG CGGCCCAGCC CCTGGAGTTT 
GCCCAGTTCC CCTTCCTACT GCGTGGCCTC CAGAAGACTG CGGACTTTGT GGAGGCCATC 
GAGGGGGCCC GGGCAGCATG CGCCGAGGCA GGTCAGGCCG GGGTGCGCGC CTACCCCAGC 
GGCTCCCCCT TCCTCTTCTG GGAGCAGTAT CTGGGCCTGC GGCGCTACTT CCTGCTGGCT 
ATCTGCATCC TGCTGGTATG CACTTTCCTC GTCTGTGCCC TGCTGCTGCT CAACCCCTGG 
ACGGCTGGCC TCATCGTACT GGTCCTGGCG ATGATGACTG TGGAGCTCTT TGGCATCATG 
GGTTTCCTGG GCATCAAACT GAGTGCCATC CCCGTGGTGA TCCTTGTGGC CTCTGTGGGC 
ATTGGTGTTG AGTTCACAGT CCACGTGGCT CTGGGCTTCC TGACCACCCA GGGTAGCCGG 
AACCTGCGGG CTGCCCACGC CCTAGAGCGC ACGTTTGCCC CAGTGACCGA TGGGGCCGTG 
TCCACGTTGC TGGGTCTGCT CATGCTTGCT GGCTCCAACT TTGACTTCAT CATACGGTAC 
TTTTTCGTGG TGCTAACTGT GCTCACACTC CTGGGCCTCC TCCATGGGCT CGTGCTGCTG 
CCTGTGCTGC TGTCCATCCT GGGCCCCCCG CCAGAGGTGG TACAGATGTA CAAGGAGAGC 
CCAGAGGTCC TCAGCCCCCC AGCTCCACGA GAAGGAGGGC TCAGGTGGGG GTTGCCCCCC 
ACCCTGCCCC AGAGCTTTGC CAGAGTGACT ACCTCCATGA CGGTGGCCCT CCACCCACCC 
CCACTGCCCG GTGCCTACAT CCACCCAGCC TCTGACGAGC CCACTTGGTC CCCTGTTGCC 
ACATCAGCTG CCAGCGGCTC CAGCAACCTC AGTTCCAGGG GACCATGTCC AGCCACTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283733.1
CDS	226..3588
Translation	MLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_022428025.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022428025.1

ORF Insert Sequence:

ATGCTGATAC AGACCCCACG CCAGGAAGGG GAGAACGTCC TCACCCCCGA GGCGCTCGGC 
CTCCACCTCC AGGCAGCCCT CACCGCCAGT AAAGTGCAAG TATCACTCTA CGGAAAGTCC 
TGGGATTTGA ACAAAATCTG CTACAAGTCA GGGATTCCCC TAATTGAAAA TGGAATGATT 
GAGCGGATGA TAGAGAAGCT GTTTCCGTGC GTGATCCTCA CCCCCCTCGA CTGCTTCTGG 
GAGGGCGCCA AGCTCCAAGG GGGCTCAGCC TACTTGCCTG GCCGCCCTGA CATCCAGTGG 
ACCAACCTGG ATCCAGAGCA GCTGCTGGAG GAGCTGGGGC CCTTTGCCTC CCTCGAGGGC 
TTCCGGGAGC TGCTAGACAA GGCACAGGTG GGCCAGGCCT ATGTGGGGCG GCCCTGTCTG 
CACCCTGACG ACCTCCACTG CCCGCCTAGT GCCCCTAACC ATCACAGCAA GCAGGCTCCC 
AACGTGGCTC AGGAGTTGAG TGGGGGCTGT CACGGCTTTT CCCACAAGTT CATGCACTGG 
CAGGAGGAAC TGCTGCTGGG GGGCATGGCC AGAGACCCCC AAGGACAGCT GCTGAGGGCG 
GAGGCCCTGC AGAGCACCTT CCTGCTGATG AGTCCCCGCC AGCTGTACGA GCACTTCCGG 
GGCGACTACC AGACGCATGA CATCGGCTGG AGCGAGGAGC AGGCGGGGAC AGTGCTACAG 
GCCTGGCAGC GGCGCTTCGT GCAGGTCAGT GTGCGGATGG GCAAGGGCGG TGCCCGAGGC 
TGCTCCCTCC TCCTGCCCCT CAGATCCACC CTGTCTCTGC AGCTGGCTCA GGAGGCCCTG 
CCTCAGAATT CATCCCAGCA GATCCATGCC TTTTCCTCCA CCACTCTGGA TGACATCCTG 
CATGCTTTCT CTGAAGTCAG TGCTGCCCGT GTGGTCGGAG GCTATCTGCT CATGCTAGCC 
TATGCTTGCG TGACGATGCT GCGCTGGGAC TGTGCCCAGT CCCAGGGTGC CGTGGGCCTT 
GCGGGGGTGC TGCTGGTAGC CCTGGCGGTG GCCTCGGGCC TTGGGCTCTG CGCCCTGCTG 
GGCATCGCCT TCAATGCCGC CACTACCCAG GTGCTGCCCT TCTTGGCACT GGGCATCGGC 
GTGGATGACA TATTCCTGCT GGCACATGCC TTCACAGAGG CTCCACCTGG CACCCCTCTC 
CAGGAGCGCA CAGGTGAGTG TCTGCAGCGC ACGGGCACCA GCGTCGCACT CACGTCCATC 
AGCCACATGG TTGCCTTCTT CATGGCCGCC CTGGTTCCCA TCCCGGCCCT GCGGGCCTTC 
TCCCTGCAGG CAGCCATAGT CGTTGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GTCTGGACCT GCACCGACGC CACTGCCAGC GCCTTGACGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC GCGGGTAATT CAGATCCTGC CCCAGGAACT GGGGGACGGG 
ACGGTGCCAG TGGGCATCGC CCACCTGACC GCCACGGTTC AAGCCTTTGC CCACTGCGAA 
GCCGGCAGCC AGCACGTCGT CACCATCCTG CCTCCCCGAG CCCGCTTGGT GCCCCCACCT 
TCTGACCCAC TGGGCTCTGA GCTCTTCAGC CCAGGAGGAT CCACACGGGA CCTTCTAGGC 
CAAGAGGAGG GGACGAGACA AAAGGCAACC TGCAGCTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TTGCCCGCTC TCAGTTTGCA CCCTTGCTGC TCCAGTCACA CAGCAAGGCC 
ATCGTGCTGG TGCTCTTTGG GGCTCTCCTG GGCCTGAGCC TCTACGGAGC AACCTTGGTG 
CAGGATGGGC TGGCCCTGAC GGATGTGGTG CCTCGGGGCA CCAAGGAGCA CGCCTTCCTG 
AGCGCCCAGC TCAGGTACTT CTCCCTGTAC GAGGTGGCCC TGGTGACGCA GGGTGGCTTT 
GACTACGCCC ACTCCCAACG CGCCCTCTTT GATCTGCACC AGCGCTTCAG CTCTCTCAAG 
GCCGTGCTGC CCACACCTGC CACCCAGGCG CCGCGCACCT GGCTACACTA TTACCGGAAC 
TGGCTCCTGG GAATCCAGGC CGCCTTTGAC CAGGACTGGG CTTCTGGGCG CATTAGCCGC 
CACTCGTGCC GCAATGGCTC CGAGGACGGG GCCCTGGCCT ACAAGCTGCT CCTCCAGACC 
GGGGATGCCC AAGAGCCTCT GGATTTCAGC CAGCTGACCA CACGGAAGCT GGTAGACAAG 
GAGGGGCTGA TTGCACCTGA GCTCTTCTAC GTGGGGCTGA CCATGTGGGT AAGCAGTGAC 
CCGCTGGGCC TGGCGGCCTC ACAGGCCAAC TTCTACCCCC CACCTCCCGA GTGGCTGCAC 
GACAAGTACG ACACCACCGG GGAGAACCTT CGCATCCCGG CGGCCCAGCC CCTGGAGTTT 
GCCCAGTTCC CCTTCCTACT GCGTGGCCTC CAGAAGACTG CGGACTTTGT GGAGGCCATC 
GAGGGGGCCC GGGCAGCATG CGCCGAGGCA GGTCAGGCCG GGGTGCGCGC CTACCCCAGC 
GGCTCCCCCT TCCTCTTCTG GGAGCAGTAT CTGGGCCTGC GGCGCTACTT CCTGCTGGCT 
ATCTGCATCC TGCTGGTATG CACTTTCCTC GTCTGTGCCC TGCTGCTGCT CAACCCCTGG 
ACGGCTGGCC TCATCGTACT GGTCCTGGCG ATGATGACTG TGGAGCTCTT TGGCATCATG 
GGTTTCCTGG GCATCAAACT GAGTGCCATC CCCGTGGTGA TCCTTGTGGC CTCTGTGGGC 
ATTGGTGTTG AGTTCACAGT CCACGTGGCT CTGGGCTTCC TGACCACCCA GGGTAGCCGG 
AACCTGCGGG CTGCCCACGC CCTAGAGCGC ACGTTTGCCC CAGTGACCGA TGGGGCCGTG 
TCCACGTTGC TGGGTCTGCT CATGCTTGCT GGCTCCAACT TTGACTTCAT CATACGGTAC 
TTTTTCGTGG TGCTAACTGT GCTCACACTC CTGGGCCTCC TCCATGGGCT CGTGCTGCTG 
CCTGTGCTGC TGTCCATCCT GGGCCCCCCG CCAGAGGTGG TACAGATGTA CAAGGAGAGC 
CCAGAGGTCC TCAGCCCCCC AGCTCCACGA GAAGGAGGGC TCAGGTGGGG GTTGCCCCCC 
ACCCTGCCCC AGAGCTTTGC CAGAGTGACT ACCTCCATGA CGGTGGCCCT CCACCCACCC 
CCACTGCCCG GTGCCTACAT CCACCCAGCC TCTGACGAGC CCACTTGGTC CCCTGTTGCC 
ACATCAGCTG CCAGCGGCTC CAGCAACCTC AGTTCCAGGG GACCATGTCC AGCCACTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_014119704.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975179.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.

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CloneID	OCe40354
Clone ID Related Accession (Same CDS sequence)	XM_014119704.1 , XM_014119704.2
Accession Version	XM_014119704.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein patched homolog 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC 
CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC 
TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT 
CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC 
CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG 
CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC 
AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG 
CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC 
ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC 
TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC 
CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT 
GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC 
TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT 
CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC 
GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC 
CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC 
GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC 
CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC 
CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA 
GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC 
CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC 
ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG 
CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC 
CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC 
TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC 
AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG 
GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG 
CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG 
CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT 
TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC 
AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA 
CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC 
ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA 
CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG 
GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG 
GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG 
GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG 
CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG 
CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG 
GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC 
CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG 
ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA 
GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT 
GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC 
CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA 
CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC 
AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG 
GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC 
ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA 
CCATGTCCAG CCACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975179.1
CDS	362..4042
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_014119704.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119704.1

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC 
CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC 
TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT 
CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC 
CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG 
CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC 
AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG 
CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC 
ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC 
TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC 
CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT 
GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC 
TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT 
CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC 
GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC 
CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC 
GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC 
CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC 
CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA 
GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC 
CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC 
ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG 
CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC 
CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC 
TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC 
AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG 
GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG 
CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG 
CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT 
TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC 
AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA 
CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC 
ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA 
CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG 
GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG 
GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG 
GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG 
CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG 
CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG 
GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC 
CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG 
ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA 
GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT 
GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC 
CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA 
CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC 
AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG 
GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC 
ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA 
CCATGTCCAG CCACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_014119704.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975179.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.

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CloneID	OCe40354
Clone ID Related Accession (Same CDS sequence)	XM_014119704.1 , XM_014119704.2
Accession Version	XM_014119704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC 
CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC 
TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT 
CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC 
CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG 
CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC 
AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG 
CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC 
ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC 
TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC 
CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT 
GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC 
TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT 
CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC 
GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC 
CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC 
GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC 
CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC 
CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA 
GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC 
CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC 
ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG 
CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC 
CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC 
TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC 
AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG 
GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG 
CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG 
CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT 
TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC 
AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA 
CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC 
ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA 
CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG 
GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG 
GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG 
GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG 
CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG 
CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG 
GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC 
CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG 
ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA 
GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT 
GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC 
CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA 
CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC 
AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG 
GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC 
ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA 
CCATGTCCAG CCACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975179.1
CDS	121..3801
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_014119704.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119704.2

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATTTGAAC AAAATCTGCT ACAAGTCAGG GATTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATA GAGAAGCTGT TTCCGTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGCGCCAAG CTCCAAGGGG GCTCAGCCTA CTTGCCTGGC 
CGCCCTGACA TCCAGTGGAC CAACCTGGAT CCAGAGCAGC TGCTGGAGGA GCTGGGGCCC 
TTTGCCTCCC TCGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAT 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CGCCTAGTGC CCCTAACCAT 
CACAGCAAGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGTCA CGGCTTTTCC 
CACAAGTTCA TGCACTGGCA GGAGGAACTG CTGCTGGGGG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCGGA GGCCCTGCAG AGCACCTTCC TGCTGATGAG TCCCCGCCAG 
CTGTACGAGC ACTTCCGGGG CGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCGGGGACAG TGCTACAGGC CTGGCAGCGG CGCTTCGTGC AGGTCAGTGT GCGGATGGGC 
AAGGGCGGTG CCCGAGGCTG CTCCCTCCTC CTGCCCCTCA GATCCACCCT GTCTCTGCAG 
CTGGCTCAGG AGGCCCTGCC TCAGAATTCA TCCCAGCAGA TCCATGCCTT TTCCTCCACC 
ACTCTGGATG ACATCCTGCA TGCTTTCTCT GAAGTCAGTG CTGCCCGTGT GGTCGGAGGC 
TATCTGCTCA TGCTAGCCTA TGCTTGCGTG ACGATGCTGC GCTGGGACTG TGCCCAGTCC 
CAGGGTGCCG TGGGCCTTGC GGGGGTGCTG CTGGTAGCCC TGGCGGTGGC CTCGGGCCTT 
GGGCTCTGCG CCCTGCTGGG CATCGCCTTC AATGCCGCCA CTACCCAGGT GCTGCCCTTC 
TTGGCACTGG GCATCGGCGT GGATGACATA TTCCTGCTGG CACATGCCTT CACAGAGGCT 
CCACCTGGCA CCCCTCTCCA GGAGCGCACA GGTGAGTGTC TGCAGCGCAC GGGCACCAGC 
GTCGCACTCA CGTCCATCAG CCACATGGTT GCCTTCTTCA TGGCCGCCCT GGTTCCCATC 
CCGGCCCTGC GGGCCTTCTC CCTGCAGGCA GCCATAGTCG TTGGCTGCAA CTTTGCAGCC 
GTGATGCTTG TCTTCCCAGC GGTCCTCAGT CTGGACCTGC ACCGACGCCA CTGCCAGCGC 
CTTGACGTGC TCTGCTGCTT CTCTAGCCCC TGCTCTGCGC GGGTAATTCA GATCCTGCCC 
CAGGAACTGG GGGACGGGAC GGTGCCAGTG GGCATCGCCC ACCTGACCGC CACGGTTCAA 
GCCTTTGCCC ACTGCGAAGC CGGCAGCCAG CACGTCGTCA CCATCCTGCC TCCCCGAGCC 
CGCTTGGTGC CCCCACCTTC TGACCCACTG GGCTCTGAGC TCTTCAGCCC AGGAGGATCC 
ACACGGGACC TTCTAGGCCA AGAGGAGGGG ACGAGACAAA AGGCAACCTG CAGCTCCCTG 
CCCTGTGCCC GCTGGAATCT TGCCCATTTT GCCCGCTCTC AGTTTGCACC CTTGCTGCTC 
CAGTCACACA GCAAGGCCAT CGTGCTGGTG CTCTTTGGGG CTCTCCTGGG CCTGAGCCTC 
TACGGAGCAA CCTTGGTGCA GGATGGGCTG GCCCTGACGG ATGTGGTGCC TCGGGGCACC 
AAGGAGCACG CCTTCCTGAG CGCCCAGCTC AGGTACTTCT CCCTGTACGA GGTGGCCCTG 
GTGACGCAGG GTGGCTTTGA CTACGCCCAC TCCCAACGCG CCCTCTTTGA TCTGCACCAG 
CGCTTCAGCT CTCTCAAGGC CGTGCTGCCC ACACCTGCCA CCCAGGCGCC GCGCACCTGG 
CTACACTATT ACCGGAACTG GCTCCTGGGA ATCCAGGCCG CCTTTGACCA GGACTGGGCT 
TCTGGGCGCA TTAGCCGCCA CTCGTGCCGC AATGGCTCCG AGGACGGGGC CCTGGCCTAC 
AAGCTGCTCC TCCAGACCGG GGATGCCCAA GAGCCTCTGG ATTTCAGCCA GCTGACCACA 
CGGAAGCTGG TAGACAAGGA GGGGCTGATT GCACCTGAGC TCTTCTACGT GGGGCTGACC 
ATGTGGGTAA GCAGTGACCC GCTGGGCCTG GCGGCCTCAC AGGCCAACTT CTACCCCCCA 
CCTCCCGAGT GGCTGCACGA CAAGTACGAC ACCACCGGGG AGAACCTTCG CATCCCGGCG 
GCCCAGCCCC TGGAGTTTGC CCAGTTCCCC TTCCTACTGC GTGGCCTCCA GAAGACTGCG 
GACTTTGTGG AGGCCATCGA GGGGGCCCGG GCAGCATGCG CCGAGGCAGG TCAGGCCGGG 
GTGCGCGCCT ACCCCAGCGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGCCTGCGG 
CGCTACTTCC TGCTGGCTAT CTGCATCCTG CTGGTATGCA CTTTCCTCGT CTGTGCCCTG 
CTGCTGCTCA ACCCCTGGAC GGCTGGCCTC ATCGTACTGG TCCTGGCGAT GATGACTGTG 
GAGCTCTTTG GCATCATGGG TTTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTGATC 
CTTGTGGCCT CTGTGGGCAT TGGTGTTGAG TTCACAGTCC ACGTGGCTCT GGGCTTCCTG 
ACCACCCAGG GTAGCCGGAA CCTGCGGGCT GCCCACGCCC TAGAGCGCAC GTTTGCCCCA 
GTGACCGATG GGGCCGTGTC CACGTTGCTG GGTCTGCTCA TGCTTGCTGG CTCCAACTTT 
GACTTCATCA TACGGTACTT TTTCGTGGTG CTAACTGTGC TCACACTCCT GGGCCTCCTC 
CATGGGCTCG TGCTGCTGCC TGTGCTGCTG TCCATCCTGG GCCCCCCGCC AGAGGTGGTA 
CAGATGTACA AGGAGAGCCC AGAGGTCCTC AGCCCCCCAG CTCCACGAGA AGGAGGGCTC 
AGGTGGGGGT TGCCCCCCAC CCTGCCCCAG AGCTTTGCCA GAGTGACTAC CTCCATGACG 
GTGGCCCTCC ACCCACCCCC ACTGCCCGGT GCCTACATCC ACCCAGCCTC TGACGAGCCC 
ACTTGGTCCC CTGTTGCCAC ATCAGCTGCC AGCGGCTCCA GCAACCTCAG TTCCAGGGGA 
CCATGTCCAG CCACTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005629080.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629137.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe17386
Clone ID Related Accession (Same CDS sequence)	XM_005629080.2 , XM_005629080.3
Accession Version	XM_005629080.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein patched homolog 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA 
GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC 
AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC 
CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC 
AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629137.1
CDS	362..4063
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_005629080.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629080.2

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA 
GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC 
AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC 
CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC 
AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005629080.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629137.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.

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CloneID	OCe17386
Clone ID Related Accession (Same CDS sequence)	XM_005629080.2 , XM_005629080.3
Accession Version	XM_005629080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA 
GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC 
AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC 
CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC 
AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629137.1
CDS	120..3821
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_005629080.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629080.3

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGGTGGT ACAGATGTAC AAGGAGAGCC CAGAGGTCCT CAGCCCCCCA 
GCTCCACGAG AAGGAGGGCT CAGGTGGGGG TTGCCCCCCA CCCTGCCCCA GAGCTTTGCC 
AGAGTGACTA CCTCCATGAC GGTGGCCCTC CACCCACCCC CACTGCCCGG TGCCTACATC 
CACCCAGCCT CTGACGAGCC CACTTGGTCC CCTGTTGCCA CATCAGCTGC CAGCGGCTCC 
AGCAACCTCA GTTCCAGGGG ACCATGTCCA GCCACTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_539636.7 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539636.3 Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.

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CloneID	OCe17387
Clone ID Related Accession (Same CDS sequence)	XM_539636.7 , XM_539636.6
Accession Version	XM_539636.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein patched homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539636.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC 
ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA 
GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG 
TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC 
CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC 
TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG 
GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC 
AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC 
ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG 
CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG 
CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT 
CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA 
GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA 
TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG 
CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC 
ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC 
TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG 
GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC 
TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC 
ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG 
ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC 
CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG 
GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT 
GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC 
GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG 
CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG 
ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC 
CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC 
CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG 
GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG 
CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG 
CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG 
GGACCATGTC CAGCCACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539636.3
CDS	1..3624
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_539636.7
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539636.7

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC 
ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA 
GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG 
TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC 
CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC 
TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG 
GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC 
AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC 
ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG 
CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG 
CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT 
CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA 
GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA 
TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG 
CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC 
ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC 
TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG 
GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC 
TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC 
ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG 
ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC 
CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG 
GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT 
GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC 
GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG 
CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG 
ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC 
CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC 
CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG 
GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG 
CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG 
CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG 
GGACCATGTC CAGCCACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_539636.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539636.3 Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.

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CloneID	OCe17387
Clone ID Related Accession (Same CDS sequence)	XM_539636.7 , XM_539636.6
Accession Version	XM_539636.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein patched homolog 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_539636.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC 
ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA 
GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG 
TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC 
CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC 
TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG 
GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC 
AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC 
ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG 
CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG 
CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT 
CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA 
GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA 
TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG 
CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC 
ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC 
TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG 
GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC 
TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC 
ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG 
ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC 
CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG 
GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT 
GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC 
GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG 
CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG 
ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC 
CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC 
CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG 
GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG 
CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG 
CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG 
GGACCATGTC CAGCCACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539636.3
CDS	361..3984
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESPEVLSPPAPREGGLRWGLPPTLPQSFARVTTSMTVALHPPPLPGAYIHPASDEPTWSPVATSAASGSSNLSSRGPCPATG

Target ORF information:

RefSeq Version	XM_539636.6
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539636.6

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGCTGGCTC AGGAGGCCCT GCCTCAGAAT TCATCCCAGC AGATCCATGC CTTTTCCTCC 
ACCACTCTGG ATGACATCCT GCATGCTTTC TCTGAAGTCA GTGCTGCCCG TGTGGTCGGA 
GGCTATCTGC TCATGCTAGC CTATGCTTGC GTGACGATGC TGCGCTGGGA CTGTGCCCAG 
TCCCAGGGTG CCGTGGGCCT TGCGGGGGTG CTGCTGGTAG CCCTGGCGGT GGCCTCGGGC 
CTTGGGCTCT GCGCCCTGCT GGGCATCGCC TTCAATGCCG CCACTACCCA GGTGCTGCCC 
TTCTTGGCAC TGGGCATCGG CGTGGATGAC ATATTCCTGC TGGCACATGC CTTCACAGAG 
GCTCCACCTG GCACCCCTCT CCAGGAGCGC ACAGGTGAGT GTCTGCAGCG CACGGGCACC 
AGCGTCGCAC TCACGTCCAT CAGCCACATG GTTGCCTTCT TCATGGCCGC CCTGGTTCCC 
ATCCCGGCCC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TCGTTGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGTCTGGACC TGCACCGACG CCACTGCCAG 
CGCCTTGACG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CGCGGGTAAT TCAGATCCTG 
CCCCAGGAAC TGGGGGACGG GACGGTGCCA GTGGGCATCG CCCACCTGAC CGCCACGGTT 
CAAGCCTTTG CCCACTGCGA AGCCGGCAGC CAGCACGTCG TCACCATCCT GCCTCCCCGA 
GCCCGCTTGG TGCCCCCACC TTCTGACCCA CTGGGCTCTG AGCTCTTCAG CCCAGGAGGA 
TCCACACGGG ACCTTCTAGG CCAAGAGGAG GGGACGAGAC AAAAGGCAAC CTGCAGCTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTTGCCCGCT CTCAGTTTGC ACCCTTGCTG 
CTCCAGTCAC ACAGCAAGGC CATCGTGCTG GTGCTCTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTACGGAG CAACCTTGGT GCAGGATGGG CTGGCCCTGA CGGATGTGGT GCCTCGGGGC 
ACCAAGGAGC ACGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACGC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCTCTCAA GGCCGTGCTG CCCACACCTG CCACCCAGGC GCCGCGCACC 
TGGCTACACT ATTACCGGAA CTGGCTCCTG GGAATCCAGG CCGCCTTTGA CCAGGACTGG 
GCTTCTGGGC GCATTAGCCG CCACTCGTGC CGCAATGGCT CCGAGGACGG GGCCCTGGCC 
TACAAGCTGC TCCTCCAGAC CGGGGATGCC CAAGAGCCTC TGGATTTCAG CCAGCTGACC 
ACACGGAAGC TGGTAGACAA GGAGGGGCTG ATTGCACCTG AGCTCTTCTA CGTGGGGCTG 
ACCATGTGGG TAAGCAGTGA CCCGCTGGGC CTGGCGGCCT CACAGGCCAA CTTCTACCCC 
CCACCTCCCG AGTGGCTGCA CGACAAGTAC GACACCACCG GGGAGAACCT TCGCATCCCG 
GCGGCCCAGC CCCTGGAGTT TGCCCAGTTC CCCTTCCTAC TGCGTGGCCT CCAGAAGACT 
GCGGACTTTG TGGAGGCCAT CGAGGGGGCC CGGGCAGCAT GCGCCGAGGC AGGTCAGGCC 
GGGGTGCGCG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGCCTG 
CGGCGCTACT TCCTGCTGGC TATCTGCATC CTGCTGGTAT GCACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGGC CTCATCGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTG 
ATCCTTGTGG CCTCTGTGGG CATTGGTGTT GAGTTCACAG TCCACGTGGC TCTGGGCTTC 
CTGACCACCC AGGGTAGCCG GAACCTGCGG GCTGCCCACG CCCTAGAGCG CACGTTTGCC 
CCAGTGACCG ATGGGGCCGT GTCCACGTTG CTGGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCATACGGTA CTTTTTCGTG GTGCTAACTG TGCTCACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCTGTGCTG CTGTCCATCC TGGGCCCCCC GCCAGAGGTG 
GTACAGATGT ACAAGGAGAG CCCAGAGGTC CTCAGCCCCC CAGCTCCACG AGAAGGAGGG 
CTCAGGTGGG GGTTGCCCCC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACGGTGGCCC TCCACCCACC CCCACTGCCC GGTGCCTACA TCCACCCAGC CTCTGACGAG 
CCCACTTGGT CCCCTGTTGC CACATCAGCT GCCAGCGGCT CCAGCAACCT CAGTTCCAGG 
GGACCATGTC CAGCCACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005629081.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629138.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.

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CloneID	OCe17388
Clone ID Related Accession (Same CDS sequence)	XM_005629081.3 , XM_005629081.2
Accession Version	XM_005629081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein patched homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629138.1
CDS	121..3594
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPELLYPMEAMVMHQ

Target ORF information:

RefSeq Version	XM_005629081.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629081.3

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005629081.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629138.1 Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe17388
Clone ID Related Accession (Same CDS sequence)	XM_005629081.3 , XM_005629081.2
Accession Version	XM_005629081.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein patched homolog 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629138.1
CDS	362..3835
Translation	MARPPPLQELPPGYTPPARATSPQIPAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIVETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGIPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSKQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQVSVRMGKGGARGCSLLLPLRSTLSLQLAQEALPQNSSQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGTPLQERTGECLQRTGTSVALTSISHMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLHRRHCQRLDVLCCFSSPCSARVIQILPQELGDGTVPVGIAHLTATVQAFAHCEAGSQHVVTILPPRARLVPPPSDPLGSELFSPGGSTRDLLGQEEGTRQKATCSSLPCARWNLAHFARSQFAPLLLQSHSKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPTPATQAPRTWLHYYRNWLLGIQAAFDQDWASGRISRHSCRNGSEDGALAYKLLLQTGDAQEPLDFSQLTTRKLVDKEGLIAPELFYVGLTMWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVRAYPSGSPFLFWEQYLGLRRYFLLAICILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALERTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLVLLPVLLSILGPPPELLYPMEAMVMHQ

Target ORF information:

RefSeq Version	XM_005629081.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris patched 2 (PTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629081.2

ORF Insert Sequence:

ATGGCCCGGC CGCCGCCGCT CCAGGAGCTG CCCCCTGGCT ATACACCCCC AGCTCGAGCC 
ACGTCGCCCC AGATCCCAGC CGGGAGCCTG AAGGCTCCAC TCTGGCTTCG CGCTTACTTC 
CAGGGCCTGC TCTTCTCTCT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGCCTCT TGGCCTTCGG AGCCCTGGCA CTGGGTCTTC GCGTGGCCAT CGTTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAGTT GCATTACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCATAC ACCTCCCAGA TGCTGATACA GACCCCACGC 
CAGGAAGGGG AGAACGTCCT CACCCCCGAG GCGCTCGGCC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCACTCTAC GGAAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAGTCAG GGATTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT AGAGAAGCTG 
TTTCCGTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGCGCCAA GCTCCAAGGG 
GGCTCAGCCT ACTTGCCTGG CCGCCCTGAC ATCCAGTGGA CCAACCTGGA TCCAGAGCAG 
CTGCTGGAGG AGCTGGGGCC CTTTGCCTCC CTCGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCGCCTAGTG CCCCTAACCA TCACAGCAAG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGTC ACGGCTTTTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTGCTGGGG 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCGG AGGCCCTGCA GAGCACCTTC 
CTGCTGATGA GTCCCCGCCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCGGGGACA GTGCTACAGG CCTGGCAGCG GCGCTTCGTG 
CAGGTCAGTG TGCGGATGGG CAAGGGCGGT GCCCGAGGCT GCTCCCTCCT CCTGCCCCTC 
AGATCCACCC TGTCTCTGCA GCTGGCTCAG GAGGCCCTGC CTCAGAATTC ATCCCAGCAG 
ATCCATGCCT TTTCCTCCAC CACTCTGGAT GACATCCTGC ATGCTTTCTC TGAAGTCAGT 
GCTGCCCGTG TGGTCGGAGG CTATCTGCTC ATGCTAGCCT ATGCTTGCGT GACGATGCTG 
CGCTGGGACT GTGCCCAGTC CCAGGGTGCC GTGGGCCTTG CGGGGGTGCT GCTGGTAGCC 
CTGGCGGTGG CCTCGGGCCT TGGGCTCTGC GCCCTGCTGG GCATCGCCTT CAATGCCGCC 
ACTACCCAGG TGCTGCCCTT CTTGGCACTG GGCATCGGCG TGGATGACAT ATTCCTGCTG 
GCACATGCCT TCACAGAGGC TCCACCTGGC ACCCCTCTCC AGGAGCGCAC AGGTGAGTGT 
CTGCAGCGCA CGGGCACCAG CGTCGCACTC ACGTCCATCA GCCACATGGT TGCCTTCTTC 
ATGGCCGCCC TGGTTCCCAT CCCGGCCCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTC 
GTTGGCTGCA ACTTTGCAGC CGTGATGCTT GTCTTCCCAG CGGTCCTCAG TCTGGACCTG 
CACCGACGCC ACTGCCAGCG CCTTGACGTG CTCTGCTGCT TCTCTAGCCC CTGCTCTGCG 
CGGGTAATTC AGATCCTGCC CCAGGAACTG GGGGACGGGA CGGTGCCAGT GGGCATCGCC 
CACCTGACCG CCACGGTTCA AGCCTTTGCC CACTGCGAAG CCGGCAGCCA GCACGTCGTC 
ACCATCCTGC CTCCCCGAGC CCGCTTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG 
CTCTTCAGCC CAGGAGGATC CACACGGGAC CTTCTAGGCC AAGAGGAGGG GACGAGACAA 
AAGGCAACCT GCAGCTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT TGCCCGCTCT 
CAGTTTGCAC CCTTGCTGCT CCAGTCACAC AGCAAGGCCA TCGTGCTGGT GCTCTTTGGG 
GCTCTCCTGG GCCTGAGCCT CTACGGAGCA ACCTTGGTGC AGGATGGGCT GGCCCTGACG 
GATGTGGTGC CTCGGGGCAC CAAGGAGCAC GCCTTCCTGA GCGCCCAGCT CAGGTACTTC 
TCCCTGTACG AGGTGGCCCT GGTGACGCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC 
GCCCTCTTTG ATCTGCACCA GCGCTTCAGC TCTCTCAAGG CCGTGCTGCC CACACCTGCC 
ACCCAGGCGC CGCGCACCTG GCTACACTAT TACCGGAACT GGCTCCTGGG AATCCAGGCC 
GCCTTTGACC AGGACTGGGC TTCTGGGCGC ATTAGCCGCC ACTCGTGCCG CAATGGCTCC 
GAGGACGGGG CCCTGGCCTA CAAGCTGCTC CTCCAGACCG GGGATGCCCA AGAGCCTCTG 
GATTTCAGCC AGCTGACCAC ACGGAAGCTG GTAGACAAGG AGGGGCTGAT TGCACCTGAG 
CTCTTCTACG TGGGGCTGAC CATGTGGGTA AGCAGTGACC CGCTGGGCCT GGCGGCCTCA 
CAGGCCAACT TCTACCCCCC ACCTCCCGAG TGGCTGCACG ACAAGTACGA CACCACCGGG 
GAGAACCTTC GCATCCCGGC GGCCCAGCCC CTGGAGTTTG CCCAGTTCCC CTTCCTACTG 
CGTGGCCTCC AGAAGACTGC GGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC 
GCCGAGGCAG GTCAGGCCGG GGTGCGCGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG 
GAGCAGTATC TGGGCCTGCG GCGCTACTTC CTGCTGGCTA TCTGCATCCT GCTGGTATGC 
ACTTTCCTCG TCTGTGCCCT GCTGCTGCTC AACCCCTGGA CGGCTGGCCT CATCGTACTG 
GTCCTGGCGA TGATGACTGT GGAGCTCTTT GGCATCATGG GTTTCCTGGG CATCAAACTG 
AGTGCCATCC CCGTGGTGAT CCTTGTGGCC TCTGTGGGCA TTGGTGTTGA GTTCACAGTC 
CACGTGGCTC TGGGCTTCCT GACCACCCAG GGTAGCCGGA ACCTGCGGGC TGCCCACGCC 
CTAGAGCGCA CGTTTGCCCC AGTGACCGAT GGGGCCGTGT CCACGTTGCT GGGTCTGCTC 
ATGCTTGCTG GCTCCAACTT TGACTTCATC ATACGGTACT TTTTCGTGGT GCTAACTGTG 
CTCACACTCC TGGGCCTCCT CCATGGGCTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCGC CAGAGCTCCT GTATCCTATG GAAGCCATGG TGATGCACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PTCH2
Entrez Gene ID	482519
Full Name	patched 2
General protein information	Preferred Names patched 2 Names protein patched homolog 2 patched homolog 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	PTCH2	NP_003729.3
Canis lupus familiaris (dog)	PTCH2	XP_539636.3
Mus musculus (house mouse)	Ptch2	NP_032984.1
Pan troglodytes (chimpanzee)	PTCH2	XP_003954411.1
Bos taurus (cattle)	PTCH2	XP_005204949.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PTCH2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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