PRL cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Gene Symbol	PRL
Entrez Gene ID	488241
Full Name	prolactin
Synonyms	GHA1
General protein information	Preferred Names prolactin Names prolactin growth hormone A1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA Protein Name

NM_001008275.2 NP_001008276.2 prolactin precursor
Pathways from BioSystems

mRNA	Protein	Name
NM_001008275.2	NP_001008276.2	prolactin precursor

Homologies

Rattus norvegicus (Norway rat)	Prl	NP_036761.1
Gallus gallus (chicken)	PRL	NP_990797.2
Canis lupus familiaris (dog)	PRL	NP_001008276.2
Xenopus tropicalis (tropical clawed frog)	prl.1	NP_001093699.1
Homo sapiens (human)	PRL	NP_001157030.1
Pan troglodytes (chimpanzee)	PRL	XP_518264.1
	PRL	NP_001040593.1
Bos taurus (cattle)	PRL	NP_776378.2
Mus musculus (house mouse)	Prl	NP_035294.2

Gene Ontology
Bibliography

Related articles in PubMed

Curated eutherian third party data gene data sets.
Premzl M
Data in brief6208-13(2016 Mar)

Third party data gene data set of eutherian growth hormone genes.
Premzl M
Genomics data6166-9(2015 Dec)

Testicular steroids, prolactin, relaxin and prostate gland markers in peripheral blood and seminal plasma of normal dogs and dogs with prostatic hyperplasia.
Wolf K, Kayacelebi H, Urhausen C, Piechotta M, Mischke R, Kramer S, Einspanier A, Oei CH, G?nzel-Apel A
Reproduction in domestic animals = Zuchthygiene47 Suppl 6243-6(2012 Dec)

Luteal and placental function in the bitch: spatio-temporal changes in prolactin receptor (PRLr) expression at dioestrus, pregnancy and normal and induced parturition.
Kowalewski MP, Michel E, Gram A, Boos A, Guscetti F, Hoffmann B, Aslan S, Reichler I
Reproductive biology and endocrinology : RB&E9109(2011 Aug)

Molecular cloning and expression of canine prolactin gene.
Hashimoto O, Yonezawa T, Sugiyama Y, Kawaminami M, Hasegawa Y
Experimental animals59(5)643-6(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The blood levels of multiple steroids and other hormones in adult male dogs with or without prostatic hyperplasia are reported.
Title: Testicular steroids, prolactin, relaxin and prostate gland markers in peripheral blood and seminal plasma of normal dogs and dogs with prostatic hyperplasia.

a role of PRL not only in maintaining the canine corpus luteum function but also in regulating the placental function is strongly suggested.
Title: Luteal and placental function in the bitch: spatio-temporal changes in prolactin receptor (PRLr) expression at dioestrus, pregnancy and normal and induced parturition.

The following PRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34158	NM_001008275.2 Latest version!	Canis lupus familiaris prolactin (PRL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRL ( NM_001008275.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001008276.2 Canis lupus familiaris prolactin (PRL), mRNA.

Price & Availability↑

CloneID	OCe34158
Clone ID Related Accession (Same CDS sequence)	NM_001008275.2
Accession Version	NM_001008275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	prolactin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HM369390.1. On or before Oct 2, 2010 this sequence version replaced XM_545363.2, NM_001008275.1. ##Evidence-Data-START## Transcript exon combination :: HM369390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2240903, SAMN00991609 [ECO:0000348] ##Evidence-Data-END##

ATGGATAACA AAGGGTGGTC GCTGAAAGGG TCACTCCTGC TCCTGCTGCT GCTGGTGTCA 
GACCTACTCC TGTGCCAGAG CGTGGCCTCC CTGCCCATCT GTCCCAGCGG GGCTGTCAAC 
TGCCAGGTGT CCCTCCGAGA CCTGTTTGAC CGTGCAGTCA TCCTGTCTCA CTACATCCAC 
AACCTCTCCT CGGAAATGTT CAACGAATTT GATAAAAGGT ATGCCCAGGG CCGGGGGTTC 
ATTACCAAGG CCATCAACAG CTGTCACACC TCCTCCCTCT CTACCCCTGA AGACAAGGAG 
CAAGCCCAAC AGATCCACCA TGAAGACCTT CTGAATCTGA TACTGAGGGT GCTGCGCTCC 
TGGAATGACC CCCTGTATCA TCTAGTCACA GAAGTGCGGG GGATGCAAGA AGCCCCAGAT 
GCAATTCTAT CCAGAGCCAT AGAGATTGAA GAACAAAACA GAAGACTTCT AGAGGGTATG 
GAGAAGATAG TTGGCCAGGT TCATCCTGGA ATCAGAGAAA ATGAGGTCTA CTCTGTCTGG 
TCAGGACTTC CATCCCTGCA GATGGCGGAT GAAGACACTC GCCTTTTTGC TTTTTATAAC 
CTGCTCCACT GCCTACGCAG GGATTCACAT AAGATTGACA ATTATCTCAA GCTCCTGAAG 
TGCCGAATCG TCTACGACAG CAACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008276.2
CDS	43..732
Translation	MDNKGWSLKGSLLLLLLLVSDLLLCQSVASLPICPSGAVNCQVSLRDLFDRAVILSHYIHNLSSEMFNEFDKRYAQGRGFITKAINSCHTSSLSTPEDKEQAQQIHHEDLLNLILRVLRSWNDPLYHLVTEVRGMQEAPDAILSRAIEIEEQNRRLLEGMEKIVGQVHPGIRENEVYSVWSGLPSLQMADEDTRLFAFYNLLHCLRRDSHKIDNYLKLLKCRIVYDSNC

Target ORF information:

RefSeq Version	NM_001008275.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris prolactin (PRL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008275.2

ORF Insert Sequence:

ATGGATAACA AAGGGTGGTC GCTGAAAGGG TCACTCCTGC TCCTGCTGCT GCTGGTGTCA 
GACCTACTCC TGTGCCAGAG CGTGGCCTCC CTGCCCATCT GTCCCAGCGG GGCTGTCAAC 
TGCCAGGTGT CCCTCCGAGA CCTGTTTGAC CGTGCAGTCA TCCTGTCTCA CTACATCCAC 
AACCTCTCCT CGGAAATGTT CAACGAATTT GATAAAAGGT ATGCCCAGGG CCGGGGGTTC 
ATTACCAAGG CCATCAACAG CTGTCACACC TCCTCCCTCT CTACCCCTGA AGACAAGGAG 
CAAGCCCAAC AGATCCACCA TGAAGACCTT CTGAATCTGA TACTGAGGGT GCTGCGCTCC 
TGGAATGACC CCCTGTATCA TCTAGTCACA GAAGTGCGGG GGATGCAAGA AGCCCCAGAT 
GCAATTCTAT CCAGAGCCAT AGAGATTGAA GAACAAAACA GAAGACTTCT AGAGGGTATG 
GAGAAGATAG TTGGCCAGGT TCATCCTGGA ATCAGAGAAA ATGAGGTCTA CTCTGTCTGG 
TCAGGACTTC CATCCCTGCA GATGGCGGAT GAAGACACTC GCCTTTTTGC TTTTTATAAC 
CTGCTCCACT GCCTACGCAG GGATTCACAT AAGATTGACA ATTATCTCAA GCTCCTGAAG 
TGCCGAATCG TCTACGACAG CAACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Curated eutherian third party data gene data sets.
Data in brief6208-13(2016 Mar)
Premzl M

Third party data gene data set of eutherian growth hormone genes.
Genomics data6166-9(2015 Dec)
Premzl M

Testicular steroids, prolactin, relaxin and prostate gland markers in peripheral blood and seminal plasma of normal dogs and dogs with prostatic hyperplasia.
Reproduction in domestic animals = Zuchthygiene47 Suppl 6243-6(2012 Dec)
Wolf K,Kayacelebi H,Urhausen C,Piechotta M,Mischke R,Kramer S,Einspanier A,Oei CH,G?nzel-Apel A

Luteal and placental function in the bitch: spatio-temporal changes in prolactin receptor (PRLr) expression at dioestrus, pregnancy and normal and induced parturition.
Reproductive biology and endocrinology : RB&E9109(2011 Aug)
Kowalewski MP,Michel E,Gram A,Boos A,Guscetti F,Hoffmann B,Aslan S,Reichler I

Molecular cloning and expression of canine prolactin gene.
Experimental animals59(5)643-6(2010)
Hashimoto O,Yonezawa T,Sugiyama Y,Kawaminami M,Hasegawa Y

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PRL cDNA ORF clone, Canis lupus familiaris(dog)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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