RNR1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNR1
Entrez Gene ID	816715
Full Name	ribonucleotide reductase 1
Synonyms	ATRNR1,CLS8,CRINKLY LEAVES 8,DPD2,R1,RIBONUCLEOTIDE REDUCTASE 1,RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT 1,defective in pollen organelle DNA degradation 2,ribonucleotide reductase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_127748.4	NP_179770.1	ribonucleotide reductase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RRM1	XP_534027.2
Mus musculus (house mouse)	Rrm1	NP_033129.2
Gallus gallus (chicken)	RRM1	NP_001026008.1
Saccharomyces cerevisiae (baker's yeast)	RNR3	NP_012198.3
Eremothecium gossypii	AGOS_ADL057W	NP_984039.2
Schizosaccharomyces pombe (fission yeast)	cdc22	NP_594491.1
Pan troglodytes (chimpanzee)	RRM1	XP_001160029.1
Bos taurus (cattle)	RRM1	NP_001106767.1
Rattus norvegicus (Norway rat)	Rrm1	NP_001013254.1
Caenorhabditis elegans (roundworm)	rnr-1	NP_499039.1
Saccharomyces cerevisiae (baker's yeast)	RNR1	NP_010993.1
Oryza sativa (rice)	Os06g0168600	NP_001056926.1
Danio rerio (zebrafish)	rrm1	NP_571530.1
Magnaporthe oryzae (rice blast fungus)	MGG_07000	XP_003709756.1
Neurospora crassa	NCU03539	XP_956818.1
Xenopus tropicalis (tropical clawed frog)	rrm1	NP_001120566.1
Homo sapiens (human)	RRM1	NP_001024.1
	RRM1	XP_001113010.1
Drosophila melanogaster (fruit fly)	RnrL	NP_477027.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010198	XP_554103.3
Kluyveromyces lactis	KLLA0C07887g	XP_452551.1
Arabidopsis thaliana (thale cress)	RNR1	NP_179770.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The cooperative activity of two 3'-->5' exoribonucleases in chloroplast mRNA 3' end maturation, and support the hypothesis that RNR1 plays a significant role in the destabilization of mRNAs unprotected by ribosomes.
Title: Ribonuclease II preserves chloroplast RNA homeostasis by increasing mRNA decay rates, and cooperates with polynucleotide phosphorylase in 3' end maturation.

Data show that the defects in ribonucleotide reductase DPD2 (At2g21790) indirectly compromise the activity of DPD1 nuclease (At5g26940) in plastids, suggesting a different regulation of organellar DNA degradation in pollen.
Title: Mutations defective in ribonucleotide reductase activity interfere with pollen plastid DNA degradation mediated by DPD1 exonuclease.

The following RNR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb07621	NM_127748.4 Latest version!	Arabidopsis thaliana ribonucleotide reductase 1 (RNR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNR1 ( NM_127748.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_179770.1 Arabidopsis thaliana ribonucleotide reductase 1 (RNR1), mRNA.

Price & Availability↑

CloneID	OAb07621
Clone ID Related Accession (Same CDS sequence)	NM_127748.4
Accession Version	NM_127748.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ribonucleotide reductase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_127748.3.

ATGTATGTAG TGAAGCGTGA CGGAAGACAG GAAACTGTTC ATTTCGATAA GATTACTGCG 
AGGCTTAAGA AACTTAGCTA TGGGCTTAGC AGTGACCATT GTGACCCTGT CCTCGTTGCT 
CAGAAGGTCT GTGCCGGTGT CTATAAAGGA GTCACTACGA GTCAACTTGA TGAGTTGGCT 
GCTGAAACTG CTGCTGCTAT GACTTGTAAC CATCCTGATT ATGCATCTCT TGCTGCTAGG 
ATTGCTGTGT CGAATCTCCA CAAGAACACT AAGAAGTCAT TTTCTGAGAC GATTAAGGAT 
ATGTTCTATC ATGTCAATGA TAGATCTGGA CTAAAGTCCC CACTAATAGC CGATGATGTG 
TTTGAGATAA TTATGCAGAA CGCTGCTCGT TTGGACAGTG AGATCATCTA TGACCGTGAT 
TTTGAATATG ATTACTTTGG ATTTAAAACT CTTGAGAGAT CGTACCTCTT GAAAGTCCAA 
GGGACTGTTG TTGAAAGGCC TCAACACATG CTGATGAGGG TTGCTGTTGG GATCCACAAG 
GATGATATTG ATTCCGTGAT CCAAACCTAC CATTTGATGT CTCAGAGATG GTTCACTCAT 
GCATCTCCTA CTCTCTTCAA CGCAGGAACT CCAAGGCCTC AATTAAGTAG CTGCTTTCTA 
GTCTGCATGA AAGATGATAG CATTGAGGGC ATATATGAAA CACTCAAAGA GTGTGCTGTT 
ATAAGCAAAT CTGCTGGGGG TATTGGTGTT TCAGTTCATA ATATTCGTGC TACCGGAAGT 
TACATTCGTG GCACAAATGG AACATCTAAT GGTATTGTTC CTATGCTGCG TGTATTCAAC 
GATACAGCTC GTTATGTTGA CCAAGGAGGA GGCAAGAGAA AGGGAGCCTT TGCTGTTTAC 
CTGGAGCCAT GGCATGCTGA TGTCTATGAG TTTCTGGAGC TGCGAAAGAA CCATGGAAAG 
GAAGAACACA GGGCTAGAGA TTTGTTTTAT GCTCTCTGGC TTCCAGATCT TTTCATGGAG 
AGGGTCCAGA ATAATGGGCA GTGGTCACTG TTTTGTCCTA ACGAAGCTCC AGGTTTGGCA 
GATTGCTGGG GAGCTGAATT TGAGACACTG TACACTAAGT ATGAAAGAGA GGGAAAGGCC 
AAAAAGGTTG TTCAGGCGCA GCAGCTTTGG TACGAAATAT TGACATCCCA GGTAGAAACA 
GGAACACCAT ACATGCTTTT CAAGGATTCA TGCAACCGAA AAAGTAATCA GCAAAATCTG 
GGTACCATAA AGTCGTCCAA CTTATGCACT GAAATCATTG AGTACACTAG TCCAACAGAA 
ACTGCTGTGT GCAATCTTGC ATCTATTGCT TTACCCAGAT TTGTAAGGGA GAAGGGTGTC 
CCATTAGACT CTCATCCACC TAAGCTCGCT GGCAGTCTGG ACTCAAAGAA TCGTTACTTT 
GATTTTGAAA AATTAGCAGA GGTGACTGCT ACTGTTACTG TTAATCTCAA TAAGATAATA 
GATGTGAATT ACTATCCTGT GGAGACTGCA AAAACTTCAA ACATGCGTCA TAGACCTATT 
GGTATTGGTG TACAAGGCCT TGCAGATGCA TTTATCCTCC TTGGAATGCC ATTTGATTCT 
CCAGAGGCCC AACAACTGAA TAAGGATATA TTCGAAACCA TATACTACCA TGCACTCAAA 
GCATCTACAG AGCTTGCTGC AAGACTTGGC CCCTATGAAA CCTATGCTGG AAGTCCCGTG 
AGTAAGGGAA TCCTTCAACC TGACATGTGG AATGTAATTC CATCAGACCG CTGGGACTGG 
GCTGTTCTTA GAGATATGAT ATCAAAGAAT GGAGTGAGGA ACTCTCTTTT AGTAGCACCA 
ATGCCAACTG CTTCAACCAG TCAAATCCTT GGGAACAATG AATGTTTTGA GCCCTACACA 
TCAAACATCT ACAGCCGCAG AGTCTTGAGT GGTGAATTCG TAGTGGTTAA TAAGCATCTT 
CTCCATGACC TAACTGATAT GGGACTTTGG ACTCCAACGC TGAAAAACAA ATTAATTAAT 
GAGAATGGTT CTATAGTTAA TGTTGCTGAG ATACCTGATG ACTTGAAGGC GATTTACAGA 
ACTGTCTGGG AAATCAAACA GAGAACAGTG GTGGACATGG CTGCTGATCG TGGATGCTAC 
ATAGATCAAA GCCAAAGCTT AAACATACAC ATGGACAAAC CCAACTTCGC AAAACTCACT 
TCGCTACACT TCTATACTTG GAAAAAGGGT CTGAAAACCG GGATGTACTA CCTGCGATCC 
CGTGCTGCAG CTGATGCGAT AAAGTTCACC GTTGACACAG CCATGCTCAA GGAGAAGCCG 
AGTGTAGCAG AAGGAGACAA AGAAGTAGAA GAAGAGGATA ATGAAACTAA GTTGGCGCAG 
ATGGTATGTT CCTTGACAAA CCCTGAAGAG TGTTTGGCCT GCGGAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_179770.1
CDS	269..2719
Translation	MYVVKRDGRQETVHFDKITARLKKLSYGLSSDHCDPVLVAQKVCAGVYKGVTTSQLDELAAETAAAMTCNHPDYASLAARIAVSNLHKNTKKSFSETIKDMFYHVNDRSGLKSPLIADDVFEIIMQNAARLDSEIIYDRDFEYDYFGFKTLERSYLLKVQGTVVERPQHMLMRVAVGIHKDDIDSVIQTYHLMSQRWFTHASPTLFNAGTPRPQLSSCFLVCMKDDSIEGIYETLKECAVISKSAGGIGVSVHNIRATGSYIRGTNGTSNGIVPMLRVFNDTARYVDQGGGKRKGAFAVYLEPWHADVYEFLELRKNHGKEEHRARDLFYALWLPDLFMERVQNNGQWSLFCPNEAPGLADCWGAEFETLYTKYEREGKAKKVVQAQQLWYEILTSQVETGTPYMLFKDSCNRKSNQQNLGTIKSSNLCTEIIEYTSPTETAVCNLASIALPRFVREKGVPLDSHPPKLAGSLDSKNRYFDFEKLAEVTATVTVNLNKIIDVNYYPVETAKTSNMRHRPIGIGVQGLADAFILLGMPFDSPEAQQLNKDIFETIYYHALKASTELAARLGPYETYAGSPVSKGILQPDMWNVIPSDRWDWAVLRDMISKNGVRNSLLVAPMPTASTSQILGNNECFEPYTSNIYSRRVLSGEFVVVNKHLLHDLTDMGLWTPTLKNKLINENGSIVNVAEIPDDLKAIYRTVWEIKQRTVVDMAADRGCYIDQSQSLNIHMDKPNFAKLTSLHFYTWKKGLKTGMYYLRSRAAADAIKFTVDTAMLKEKPSVAEGDKEVEEEDNETKLAQMVCSLTNPEECLACGS

Target ORF information:

RefSeq Version	NM_127748.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ribonucleotide reductase 1 (RNR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_127748.4

ORF Insert Sequence:

ATGTATGTAG TGAAGCGTGA CGGAAGACAG GAAACTGTTC ATTTCGATAA GATTACTGCG 
AGGCTTAAGA AACTTAGCTA TGGGCTTAGC AGTGACCATT GTGACCCTGT CCTCGTTGCT 
CAGAAGGTCT GTGCCGGTGT CTATAAAGGA GTCACTACGA GTCAACTTGA TGAGTTGGCT 
GCTGAAACTG CTGCTGCTAT GACTTGTAAC CATCCTGATT ATGCATCTCT TGCTGCTAGG 
ATTGCTGTGT CGAATCTCCA CAAGAACACT AAGAAGTCAT TTTCTGAGAC GATTAAGGAT 
ATGTTCTATC ATGTCAATGA TAGATCTGGA CTAAAGTCCC CACTAATAGC CGATGATGTG 
TTTGAGATAA TTATGCAGAA CGCTGCTCGT TTGGACAGTG AGATCATCTA TGACCGTGAT 
TTTGAATATG ATTACTTTGG ATTTAAAACT CTTGAGAGAT CGTACCTCTT GAAAGTCCAA 
GGGACTGTTG TTGAAAGGCC TCAACACATG CTGATGAGGG TTGCTGTTGG GATCCACAAG 
GATGATATTG ATTCCGTGAT CCAAACCTAC CATTTGATGT CTCAGAGATG GTTCACTCAT 
GCATCTCCTA CTCTCTTCAA CGCAGGAACT CCAAGGCCTC AATTAAGTAG CTGCTTTCTA 
GTCTGCATGA AAGATGATAG CATTGAGGGC ATATATGAAA CACTCAAAGA GTGTGCTGTT 
ATAAGCAAAT CTGCTGGGGG TATTGGTGTT TCAGTTCATA ATATTCGTGC TACCGGAAGT 
TACATTCGTG GCACAAATGG AACATCTAAT GGTATTGTTC CTATGCTGCG TGTATTCAAC 
GATACAGCTC GTTATGTTGA CCAAGGAGGA GGCAAGAGAA AGGGAGCCTT TGCTGTTTAC 
CTGGAGCCAT GGCATGCTGA TGTCTATGAG TTTCTGGAGC TGCGAAAGAA CCATGGAAAG 
GAAGAACACA GGGCTAGAGA TTTGTTTTAT GCTCTCTGGC TTCCAGATCT TTTCATGGAG 
AGGGTCCAGA ATAATGGGCA GTGGTCACTG TTTTGTCCTA ACGAAGCTCC AGGTTTGGCA 
GATTGCTGGG GAGCTGAATT TGAGACACTG TACACTAAGT ATGAAAGAGA GGGAAAGGCC 
AAAAAGGTTG TTCAGGCGCA GCAGCTTTGG TACGAAATAT TGACATCCCA GGTAGAAACA 
GGAACACCAT ACATGCTTTT CAAGGATTCA TGCAACCGAA AAAGTAATCA GCAAAATCTG 
GGTACCATAA AGTCGTCCAA CTTATGCACT GAAATCATTG AGTACACTAG TCCAACAGAA 
ACTGCTGTGT GCAATCTTGC ATCTATTGCT TTACCCAGAT TTGTAAGGGA GAAGGGTGTC 
CCATTAGACT CTCATCCACC TAAGCTCGCT GGCAGTCTGG ACTCAAAGAA TCGTTACTTT 
GATTTTGAAA AATTAGCAGA GGTGACTGCT ACTGTTACTG TTAATCTCAA TAAGATAATA 
GATGTGAATT ACTATCCTGT GGAGACTGCA AAAACTTCAA ACATGCGTCA TAGACCTATT 
GGTATTGGTG TACAAGGCCT TGCAGATGCA TTTATCCTCC TTGGAATGCC ATTTGATTCT 
CCAGAGGCCC AACAACTGAA TAAGGATATA TTCGAAACCA TATACTACCA TGCACTCAAA 
GCATCTACAG AGCTTGCTGC AAGACTTGGC CCCTATGAAA CCTATGCTGG AAGTCCCGTG 
AGTAAGGGAA TCCTTCAACC TGACATGTGG AATGTAATTC CATCAGACCG CTGGGACTGG 
GCTGTTCTTA GAGATATGAT ATCAAAGAAT GGAGTGAGGA ACTCTCTTTT AGTAGCACCA 
ATGCCAACTG CTTCAACCAG TCAAATCCTT GGGAACAATG AATGTTTTGA GCCCTACACA 
TCAAACATCT ACAGCCGCAG AGTCTTGAGT GGTGAATTCG TAGTGGTTAA TAAGCATCTT 
CTCCATGACC TAACTGATAT GGGACTTTGG ACTCCAACGC TGAAAAACAA ATTAATTAAT 
GAGAATGGTT CTATAGTTAA TGTTGCTGAG ATACCTGATG ACTTGAAGGC GATTTACAGA 
ACTGTCTGGG AAATCAAACA GAGAACAGTG GTGGACATGG CTGCTGATCG TGGATGCTAC 
ATAGATCAAA GCCAAAGCTT AAACATACAC ATGGACAAAC CCAACTTCGC AAAACTCACT 
TCGCTACACT TCTATACTTG GAAAAAGGGT CTGAAAACCG GGATGTACTA CCTGCGATCC 
CGTGCTGCAG CTGATGCGAT AAAGTTCACC GTTGACACAG CCATGCTCAA GGAGAAGCCG 
AGTGTAGCAG AAGGAGACAA AGAAGTAGAA GAAGAGGATA ATGAAACTAA GTTGGCGCAG 
ATGGTATGTT CCTTGACAAA CCCTGAAGAG TGTTTGGCCT GCGGAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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RNR1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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