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AT2G34210 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Summary

Gene Symbol AT2G34210
Entrez Gene ID 817982
Full Name Transcription elongation factor Spt5
Synonyms F13P17.5,F13P17_5,SPT5-1
Gene Type protein-coding
Organism Arabidopsis thaliana(thale cress)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_128972.2 NP_180968.2 Transcription elongation factor Spt5
NM_001336495.1 NP_001324132.1 Transcription elongation factor Spt5

Pathways from BioSystems


Homologies


Anopheles gambiae (African malaria mosquito) AgaP_AGAP005021 XP_001231045.2
Caenorhabditis elegans (roundworm) spt-5 NP_502283.1
Xenopus tropicalis (tropical clawed frog) supt5h NP_001123796.1
Canis lupus familiaris (dog) SUPT5H XP_005616644.1
Mus musculus (house mouse) Supt5 NP_038704.1
Gallus gallus (chicken) SUPT5H NP_001026555.1
Neurospora crassa NCU06929 XP_959276.2
SUPT5H XP_001088495.1
Bos taurus (cattle) SUPT5H NP_001076908.2
Rattus norvegicus (Norway rat) Supt5h NP_001100967.2
Schizosaccharomyces pombe (fission yeast) spt5 NP_593191.1
Arabidopsis thaliana (thale cress) AT2G34210 NP_180968.2
Oryza sativa (rice) Os02g0772000 NP_001173159.1
Saccharomyces cerevisiae (baker's yeast) SPT5 NP_013703.1
Eremothecium gossypii AGOS_ADR187W NP_984283.2
Homo sapiens (human) SUPT5H NP_001104490.1
Pan troglodytes (chimpanzee) SUPT5H XP_512652.3
Danio rerio (zebrafish) supt5h NP_571748.2
Drosophila melanogaster (fruit fly) Spt5 NP_652610.1
Kluyveromyces lactis KLLA0B00891g XP_451570.1
Magnaporthe oryzae (rice blast fungus) MGG_12268 XP_003715018.1
Arabidopsis thaliana (thale cress) GTA2 NP_192575.3

Gene Ontology


Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The phosphorylation of SPT5 by CDKD;2 enables it to recruit VIP5 to regulate chromatin and transcription in Arabidopsis.
Title: Phosphorylation of SPT5 by CDKD;2 Is Required for VIP5 Recruitment and Normal Flowering in

SPT5-like protein emerged in plants as a facultative RNA polymerase elongation factor.[SPT5]
Title: RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family.

The following AT2G34210 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT2G34210 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb08718 NM_128972.2
Latest version!		 Arabidopsis thaliana Transcription elongation factor Spt5 (AT2G34210), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $510.30
$729.00
OAb36300 NM_001336495.1
Latest version!		 Arabidopsis thaliana Transcription elongation factor Spt5 (AT2G34210), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OAb08718
Clone ID Related Accession (Same CDS sequence) NM_128972.2
Accession Version NM_128972.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-13
Organism Arabidopsis thaliana(thale cress)
Product Transcription elongation factor Spt5
Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Apr 24, 2008 this sequence version replaced NM_128972.1. COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGTCTCAGT ACTCAGACGA CGATTATTCA CATGAAGATG ACTCGGAGAT GGAGGATGAG 
GACGAAGAAG ACGAATATGA ACCCAGAAGT AGCCGCAAAG GAAGATCAGG GAAGAAAAGG 
GGAAGATCAA ATTCAGATTC CGATGGTCGG CGAGGGAGTA AAAAGAAGTC TTCGGGCTCC 
GCCTTCATTG ACTGGGAGGT TGAGGTAGAT GACGACGTCG AAGACGACGA TGATGATGTG 
GACGTTGAAG ACGGTAAGCA ACAGTTGAAA TTTGGGGATT TTTCTCTTTG TTTCATTGTA 
AGTGGTGAAG CTGACCTTCC TAATGAGGAT TCTGATCATC GTAGGCAATA TTACCAACGG 
GGGTTTCATC CTCATGAAGA AGATGTCGAC GAGTTGGAAA AGAGAACTTT GGAAAGATTG 
TCTACGAAAT ATGCAAAGGA TGATTATGAG CTTGATGATG TTAACGACGT TGATCAACAA 
GCGCTTTTGC CATCAGTCCG TGACCCTAAA CTGTGGCTGG TGAAATGTGC GATTGGACGT 
GAAAGAGAAG TTGCTGTTTG TCTTATGCAA AAAATCGTAG ATAGAGGATC AGAGTTCAAG 
ATTAGATCTG CTATTGCTCT TGATCATCTT CAAAACTATG TATATATTGA AGCGGATATG 
GAAGCTCATG TGAAAGAGGC TATCAAGGGC ATGCGGAATA TTTATGCTAA TCAGAAGATT 
TTGCTTGTCC CCATAAAAGA AATGACTGCT GTTCTTTCTG TCGAGAGTAA AGCAATCGAT 
TTATCCCGTG ACTCGTGGGT TAGAATGAAA CTTGGGATAT ATAAGGGTGA TCTAGCTCAG 
GTGGTTGATG TTGATAATGT ACGTAAGAGG GTCACAGTCA AGTTGATCCC AAGAATTGAT 
CTGCAGGCTC TAGCCAATAA GCTGGAAGGA ACAGAGAATG TCAAGAAGAA AGCTTTTGCT 
CCACCTCCAC GTTTCATGAA CATTGATGAG GCAAGGGAAC TTCACATACG TGTTGAGCAC 
CGGCGTGATC CAATGACGGG TGACTACTTT GAGAATATCG GTGGAATGCT TTTCAAAGAT 
GGTTTTCTTT ACAAGAAAGT ATCGACGAAG TCCATTGCTG CTCAGAATGT AACACCAACC 
TTCGATGAAC TTGAAAGATT CAAGAGACCA AATGAAAATG GGGAGATCGA CTTCGTTGAT 
GAGTCGACTT TATTTGCAAA TAGAAAGAAG GGTCATTTCA TGAAAGGTGA TGCGGTTATT 
GTGATCAAGG GGGATCTTAA AAACTTGAAG GGCTGGATTG AGAAAGTAGA TGAAGAGAAT 
GTCCTTATCA GATCAGAGAT GAAAGATCTT CCTAATCCTA TTGCGGTTAA TGGGAGAGAG 
CTTTGTAAAT ACTTTGAACC TGGAAATTTT GTGAAGGTTG TGTCTGGCAT CCATGAAGGA 
GGAACTGGCA TGATTGTCAA AGTTGATCAG CATATGCTTA TCATTCTGTC TGATACAACC 
AAAGAACATA TTTGCGTCTT CGCTGATCAT GTTGCCAAGA GTGCAGAAGT GACTAAAGGT 
GTCACAAAAA TTGGGGACTA TGAACTTCAT GATCTTGTGA TTCTAAGTGA CTTTAGCTTT 
GGAGTAATCC TAAAACTTGA TAGTGAAGCT ATCCAGATTC TTAAAGGGGT TCCTGACAGC 
TCAGAGGTCT CTATTGTCAA AGCCAGCGAA ATAAAATACA AAATTTGGAA GAAAATCAAT 
GTGCAAGACC GTTACAAAAA TGTCGTCGCG GTGAAAGATG TTGTCAGGGT CATTGAGGGT 
CCTAGCAAAG GTAAACAAGG TCCAGTGGTG CAAATCTACA AAGGAGTGTT GTTTATACAT 
GACCGGCATA ACCTTGAACA CACTGGATTT ATCTGCACTA GATGCTCATC TTGTGTTCTT 
GCTGGTGGCA ATTTCAAGAC ACCAGCCCTG GTTCCTCCAT CTCCAAGGAG ATTCCAACGG 
GCAGACATGG GATATAACCC TGGAGCTGGA GGGAGACATC AGGGTGGACG AGGGAGAAGG 
GGTGACGATC ATTTGGTAGG TACTTATGTC AAAATTCGTC TGGGGCCTTT CAAGGGATAT 
AGTGGGCGTT TAGTTGAAGT CAAAGATAAG CTAGTGCGTG TGGAACTTGA GGCCAAGATT 
GTGACAGTTG AGCGGAAGGC GATATCAGAT ATGACTGATA ATGTAGTCGC AACCCCGCAG 
TATAATATGG GAAGCCAAAC TCCTATGCAT CCTTCCCGGA CTCCACTTCA TCCCTGTATG 
ACTCCAATGC GGCATTCTGG AGCTACACCT ATCCATGATG GAATGAGGAC ACCTATGCGT 
GGTAGAGCTT GGAATCCTTA CATGCCTATG AGTCCTCCTA GGGATAACTG GGAAGATGGG 
AACCCGGGAT CATGGGGAAC AAGTCCATAT GAAGCAGCTA CGCCTGGATC AGATTGGGGT 
AGTAGTACTC CTGGTCGTAG TAGTTACAGA GATGCCGGAA CACCAATAAA CAATGCTAAT 
GCCCCAAGTC CTATGACACC AAGCTCAACT TCCTACTTAC CTACCACACC CGGAGGACAA 
GCAATGACTC CTGGAACCGA TCTCGATGTC ATGTCTCTTG ATATAGGTGG AGATGCAGAA 
ACCAGGTTCA TACCCGGTAT ATTGGTCAAT GTACACAAAG CTGGAGAGGA TAGAAATCCC 
GGTGTTATTC GAGATGTTCT TCCGGATGGC TCCTGTGTAG TCGCACTTGG ACACAGAGGT 
GAAGGCGAGA CGATAAGAGC GACACAGAAC AAAGTGAGCC TAGTTTGTCC GAAGAAGAAC 
GAACGTGTGA AGATTCTTGG AGGCAAGTAT TGTGGTTCAA CAGCTAAGGT CATTGGTGAG 
GACGGTCAGG ACGGTATAGT TAAGCTAGAT GAAAGTCTCG ATATCAAGAT ATTGAAGCTG 
ACTATATTAG CCAAGTTAGT TCATGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_180968.2
CDS1..2970
Translation

Target ORF information:

RefSeq Version NM_128972.2
Organism Arabidopsis thaliana(thale cress)
Definition Arabidopsis thaliana Transcription elongation factor Spt5 (AT2G34210), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_128972.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGTCTCAGT ACTCAGACGA CGATTATTCA CATGAAGATG ACTCGGAGAT GGAGGATGAG 
GACGAAGAAG ACGAATATGA ACCCAGAAGT AGCCGCAAAG GAAGATCAGG GAAGAAAAGG 
GGAAGATCAA ATTCAGATTC CGATGGTCGG CGAGGGAGTA AAAAGAAGTC TTCGGGCTCC 
GCCTTCATTG ACTGGGAGGT TGAGGTAGAT GACGACGTCG AAGACGACGA TGATGATGTG 
GACGTTGAAG ACGGTAAGCA ACAGTTGAAA TTTGGGGATT TTTCTCTTTG TTTCATTGTA 
AGTGGTGAAG CTGACCTTCC TAATGAGGAT TCTGATCATC GTAGGCAATA TTACCAACGG 
GGGTTTCATC CTCATGAAGA AGATGTCGAC GAGTTGGAAA AGAGAACTTT GGAAAGATTG 
TCTACGAAAT ATGCAAAGGA TGATTATGAG CTTGATGATG TTAACGACGT TGATCAACAA 
GCGCTTTTGC CATCAGTCCG TGACCCTAAA CTGTGGCTGG TGAAATGTGC GATTGGACGT 
GAAAGAGAAG TTGCTGTTTG TCTTATGCAA AAAATCGTAG ATAGAGGATC AGAGTTCAAG 
ATTAGATCTG CTATTGCTCT TGATCATCTT CAAAACTATG TATATATTGA AGCGGATATG 
GAAGCTCATG TGAAAGAGGC TATCAAGGGC ATGCGGAATA TTTATGCTAA TCAGAAGATT 
TTGCTTGTCC CCATAAAAGA AATGACTGCT GTTCTTTCTG TCGAGAGTAA AGCAATCGAT 
TTATCCCGTG ACTCGTGGGT TAGAATGAAA CTTGGGATAT ATAAGGGTGA TCTAGCTCAG 
GTGGTTGATG TTGATAATGT ACGTAAGAGG GTCACAGTCA AGTTGATCCC AAGAATTGAT 
CTGCAGGCTC TAGCCAATAA GCTGGAAGGA ACAGAGAATG TCAAGAAGAA AGCTTTTGCT 
CCACCTCCAC GTTTCATGAA CATTGATGAG GCAAGGGAAC TTCACATACG TGTTGAGCAC 
CGGCGTGATC CAATGACGGG TGACTACTTT GAGAATATCG GTGGAATGCT TTTCAAAGAT 
GGTTTTCTTT ACAAGAAAGT ATCGACGAAG TCCATTGCTG CTCAGAATGT AACACCAACC 
TTCGATGAAC TTGAAAGATT CAAGAGACCA AATGAAAATG GGGAGATCGA CTTCGTTGAT 
GAGTCGACTT TATTTGCAAA TAGAAAGAAG GGTCATTTCA TGAAAGGTGA TGCGGTTATT 
GTGATCAAGG GGGATCTTAA AAACTTGAAG GGCTGGATTG AGAAAGTAGA TGAAGAGAAT 
GTCCTTATCA GATCAGAGAT GAAAGATCTT CCTAATCCTA TTGCGGTTAA TGGGAGAGAG 
CTTTGTAAAT ACTTTGAACC TGGAAATTTT GTGAAGGTTG TGTCTGGCAT CCATGAAGGA 
GGAACTGGCA TGATTGTCAA AGTTGATCAG CATATGCTTA TCATTCTGTC TGATACAACC 
AAAGAACATA TTTGCGTCTT CGCTGATCAT GTTGCCAAGA GTGCAGAAGT GACTAAAGGT 
GTCACAAAAA TTGGGGACTA TGAACTTCAT GATCTTGTGA TTCTAAGTGA CTTTAGCTTT 
GGAGTAATCC TAAAACTTGA TAGTGAAGCT ATCCAGATTC TTAAAGGGGT TCCTGACAGC 
TCAGAGGTCT CTATTGTCAA AGCCAGCGAA ATAAAATACA AAATTTGGAA GAAAATCAAT 
GTGCAAGACC GTTACAAAAA TGTCGTCGCG GTGAAAGATG TTGTCAGGGT CATTGAGGGT 
CCTAGCAAAG GTAAACAAGG TCCAGTGGTG CAAATCTACA AAGGAGTGTT GTTTATACAT 
GACCGGCATA ACCTTGAACA CACTGGATTT ATCTGCACTA GATGCTCATC TTGTGTTCTT 
GCTGGTGGCA ATTTCAAGAC ACCAGCCCTG GTTCCTCCAT CTCCAAGGAG ATTCCAACGG 
GCAGACATGG GATATAACCC TGGAGCTGGA GGGAGACATC AGGGTGGACG AGGGAGAAGG 
GGTGACGATC ATTTGGTAGG TACTTATGTC AAAATTCGTC TGGGGCCTTT CAAGGGATAT 
AGTGGGCGTT TAGTTGAAGT CAAAGATAAG CTAGTGCGTG TGGAACTTGA GGCCAAGATT 
GTGACAGTTG AGCGGAAGGC GATATCAGAT ATGACTGATA ATGTAGTCGC AACCCCGCAG 
TATAATATGG GAAGCCAAAC TCCTATGCAT CCTTCCCGGA CTCCACTTCA TCCCTGTATG 
ACTCCAATGC GGCATTCTGG AGCTACACCT ATCCATGATG GAATGAGGAC ACCTATGCGT 
GGTAGAGCTT GGAATCCTTA CATGCCTATG AGTCCTCCTA GGGATAACTG GGAAGATGGG 
AACCCGGGAT CATGGGGAAC AAGTCCATAT GAAGCAGCTA CGCCTGGATC AGATTGGGGT 
AGTAGTACTC CTGGTCGTAG TAGTTACAGA GATGCCGGAA CACCAATAAA CAATGCTAAT 
GCCCCAAGTC CTATGACACC AAGCTCAACT TCCTACTTAC CTACCACACC CGGAGGACAA 
GCAATGACTC CTGGAACCGA TCTCGATGTC ATGTCTCTTG ATATAGGTGG AGATGCAGAA 
ACCAGGTTCA TACCCGGTAT ATTGGTCAAT GTACACAAAG CTGGAGAGGA TAGAAATCCC 
GGTGTTATTC GAGATGTTCT TCCGGATGGC TCCTGTGTAG TCGCACTTGG ACACAGAGGT 
GAAGGCGAGA CGATAAGAGC GACACAGAAC AAAGTGAGCC TAGTTTGTCC GAAGAAGAAC 
GAACGTGTGA AGATTCTTGG AGGCAAGTAT TGTGGTTCAA CAGCTAAGGT CATTGGTGAG 
GACGGTCAGG ACGGTATAGT TAAGCTAGAT GAAAGTCTCG ATATCAAGAT ATTGAAGCTG 
ACTATATTAG CCAAGTTAGT TCATGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OAb36300
Clone ID Related Accession (Same CDS sequence) NM_001336495.1
Accession Version NM_001336495.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-13
Organism Arabidopsis thaliana(thale cress)
Product Transcription elongation factor Spt5
Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGTCTCAGT ACTCAGACGA CGATTATTCA CATGAAGATG ACTCGGAGAT GGAGGATGAG 
GACGAAGAAG ACGAATATGA ACCCAGAAGT AGCCGCAAAG GAAGATCAGG GAAGAAAAGG 
GGAAGATCAA ATTCAGATTC CGATGGTCGG CGAGGGAGTA AAAAGAAGTC TTCGGGCTCC 
GCCTTCATTG ACTGGGAGGT TGAGGTAGAT GACGACGTCG AAGACGACGA TGATGATGTG 
GACGTTGAAG ACGGTTTCAT TGTAAGTGGT GAAGCTGACC TTCCTAATGA GGATTCTGAT 
CATCGTAGGC AATATTACCA ACGGGGGTTT CATCCTCATG AAGAAGATGT CGACGAGTTG 
GAAAAGAGAA CTTTGGAAAG ATTGTCTACG AAATATGCAA AGGATGATTA TGAGCTTGAT 
GATGTTAACG ACGTTGATCA ACAAGCGCTT TTGCCATCAG TCCGTGACCC TAAACTGTGG 
CTGGTGAAAT GTGCGATTGG ACGTGAAAGA GAAGTTGCTG TTTGTCTTAT GCAAAAAATC 
GTAGATAGAG GATCAGAGTT CAAGATTAGA TCTGCTATTG CTCTTGATCA TCTTCAAAAC 
TATGTATATA TTGAAGCGGA TATGGAAGCT CATGTGAAAG AGGCTATCAA GGGCATGCGG 
AATATTTATG CTAATCAGAA GATTTTGCTT GTCCCCATAA AAGAAATGAC TGCTGTTCTT 
TCTGTCGAGA GTAAAGCAAT CGATTTATCC CGTGACTCGT GGGTTAGAAT GAAACTTGGG 
ATATATAAGG GTGATCTAGC TCAGGTGGTT GATGTTGATA ATGTACGTAA GAGGGTCACA 
GTCAAGTTGA TCCCAAGAAT TGATCTGCAG GCTCTAGCCA ATAAGCTGGA AGGAACAGAG 
AATGTCAAGA AGAAAGCTTT TGCTCCACCT CCACGTTTCA TGAACATTGA TGAGGCAAGG 
GAACTTCACA TACGTGTTGA GCACCGGCGT GATCCAATGA CGGGTGACTA CTTTGAGAAT 
ATCGGTGGAA TGCTTTTCAA AGATGGTTTT CTTTACAAGA AAGTATCGAC GAAGTCCATT 
GCTGCTCAGA ATGTAACACC AACCTTCGAT GAACTTGAAA GATTCAAGAG ACCAAATGAA 
AATGGGGAGA TCGACTTCGT TGATGAGTCG ACTTTATTTG CAAATAGAAA GAAGGGTCAT 
TTCATGAAAG GTGATGCGGT TATTGTGATC AAGGGGGATC TTAAAAACTT GAAGGGCTGG 
ATTGAGAAAG TAGATGAAGA GAATGTCCTT ATCAGATCAG AGATGAAAGA TCTTCCTAAT 
CCTATTGCGG TTAATGGGAG AGAGCTTTGT AAATACTTTG AACCTGGAAA TTTTGTGAAG 
GTTGTGTCTG GCATCCATGA AGGAGGAACT GGCATGATTG TCAAAGTTGA TCAGCATATG 
CTTATCATTC TGTCTGATAC AACCAAAGAA CATATTTGCG TCTTCGCTGA TCATGTTGCC 
AAGAGTGCAG AAGTGACTAA AGGTGTCACA AAAATTGGGG ACTATGAACT TCATGATCTT 
GTGATTCTAA GTGACTTTAG CTTTGGAGTA ATCCTAAAAC TTGATAGTGA AGCTATCCAG 
ATTCTTAAAG GGGTTCCTGA CAGCTCAGAG GTCTCTATTG TCAAAGCCAG CGAAATAAAA 
TACAAAATTT GGAAGAAAAT CAATGTGCAA GACCGTTACA AAAATGTCGT CGCGGTGAAA 
GATGTTGTCA GGGTCATTGA GGGTCCTAGC AAAGGTAAAC AAGGTCCAGT GGTGCAAATC 
TACAAAGGAG TGTTGTTTAT ACATGACCGG CATAACCTTG AACACACTGG ATTTATCTGC 
ACTAGATGCT CATCTTGTGT TCTTGCTGGT GGCAATTTCA AGACACCAGC CCTGGTTCCT 
CCATCTCCAA GGAGATTCCA ACGGGCAGAC ATGGGATATA ACCCTGGAGC TGGAGGGAGA 
CATCAGGGTG GACGAGGGAG AAGGGGTGAC GATCATTTGG TAGGTACTTA TGTCAAAATT 
CGTCTGGGGC CTTTCAAGGG ATATAGTGGG CGTTTAGTTG AAGTCAAAGA TAAGCTAGTG 
CGTGTGGAAC TTGAGGCCAA GATTGTGACA GTTGAGCGGA AGGCGATATC AGATATGACT 
GATAATGTAG TCGCAACCCC GCAGTATAAT ATGGGAAGCC AAACTCCTAT GCATCCTTCC 
CGGACTCCAC TTCATCCCTG TATGACTCCA ATGCGGCATT CTGGAGCTAC ACCTATCCAT 
GATGGAATGA GGACACCTAT GCGTGGTAGA GCTTGGAATC CTTACATGCC TATGAGTCCT 
CCTAGGGATA ACTGGGAAGA TGGGAACCCG GGATCATGGG GAACAAGTCC ATATGAAGCA 
GCTACGCCTG GATCAGATTG GGGTAGTAGT ACTCCTGGTC GTAGTAGTTA CAGAGATGCC 
GGAACACCAA TAAACAATGC TAATGCCCCA AGTCCTATGA CACCAAGCTC AACTTCCTAC 
TTACCTACCA CACCCGGAGG ACAAGCAATG ACTCCTGGAA CCGATCTCGA TGTCATGTCT 
CTTGATATAG GTGGAGATGC AGAAACCAGG TTCATACCCG GTATATTGGT CAATGTACAC 
AAAGCTGGAG AGGATAGAAA TCCCGGTGTT ATTCGAGATG TTCTTCCGGA TGGCTCCTGT 
GTAGTCGCAC TTGGACACAG AGGTGAAGGC GAGACGATAA GAGCGACACA GAACAAAGTG 
AGCCTAGTTT GTCCGAAGAA GAACGAACGT GTGAAGATTC TTGGAGGCAA GTATTGTGGT 
TCAACAGCTA AGGTCATTGG TGAGGACGGT CAGGACGGTA TAGTTAAGCT AGATGAAAGT 
CTCGATATCA AGATATTGAA GCTGACTATA TTAGCCAAGT TAGTTCATGA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001324132.1
CDS1..2934
Translation

Target ORF information:

RefSeq Version NM_001336495.1
Organism Arabidopsis thaliana(thale cress)
Definition Arabidopsis thaliana Transcription elongation factor Spt5 (AT2G34210), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001336495.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGTCTCAGT ACTCAGACGA CGATTATTCA CATGAAGATG ACTCGGAGAT GGAGGATGAG 
GACGAAGAAG ACGAATATGA ACCCAGAAGT AGCCGCAAAG GAAGATCAGG GAAGAAAAGG 
GGAAGATCAA ATTCAGATTC CGATGGTCGG CGAGGGAGTA AAAAGAAGTC TTCGGGCTCC 
GCCTTCATTG ACTGGGAGGT TGAGGTAGAT GACGACGTCG AAGACGACGA TGATGATGTG 
GACGTTGAAG ACGGTTTCAT TGTAAGTGGT GAAGCTGACC TTCCTAATGA GGATTCTGAT 
CATCGTAGGC AATATTACCA ACGGGGGTTT CATCCTCATG AAGAAGATGT CGACGAGTTG 
GAAAAGAGAA CTTTGGAAAG ATTGTCTACG AAATATGCAA AGGATGATTA TGAGCTTGAT 
GATGTTAACG ACGTTGATCA ACAAGCGCTT TTGCCATCAG TCCGTGACCC TAAACTGTGG 
CTGGTGAAAT GTGCGATTGG ACGTGAAAGA GAAGTTGCTG TTTGTCTTAT GCAAAAAATC 
GTAGATAGAG GATCAGAGTT CAAGATTAGA TCTGCTATTG CTCTTGATCA TCTTCAAAAC 
TATGTATATA TTGAAGCGGA TATGGAAGCT CATGTGAAAG AGGCTATCAA GGGCATGCGG 
AATATTTATG CTAATCAGAA GATTTTGCTT GTCCCCATAA AAGAAATGAC TGCTGTTCTT 
TCTGTCGAGA GTAAAGCAAT CGATTTATCC CGTGACTCGT GGGTTAGAAT GAAACTTGGG 
ATATATAAGG GTGATCTAGC TCAGGTGGTT GATGTTGATA ATGTACGTAA GAGGGTCACA 
GTCAAGTTGA TCCCAAGAAT TGATCTGCAG GCTCTAGCCA ATAAGCTGGA AGGAACAGAG 
AATGTCAAGA AGAAAGCTTT TGCTCCACCT CCACGTTTCA TGAACATTGA TGAGGCAAGG 
GAACTTCACA TACGTGTTGA GCACCGGCGT GATCCAATGA CGGGTGACTA CTTTGAGAAT 
ATCGGTGGAA TGCTTTTCAA AGATGGTTTT CTTTACAAGA AAGTATCGAC GAAGTCCATT 
GCTGCTCAGA ATGTAACACC AACCTTCGAT GAACTTGAAA GATTCAAGAG ACCAAATGAA 
AATGGGGAGA TCGACTTCGT TGATGAGTCG ACTTTATTTG CAAATAGAAA GAAGGGTCAT 
TTCATGAAAG GTGATGCGGT TATTGTGATC AAGGGGGATC TTAAAAACTT GAAGGGCTGG 
ATTGAGAAAG TAGATGAAGA GAATGTCCTT ATCAGATCAG AGATGAAAGA TCTTCCTAAT 
CCTATTGCGG TTAATGGGAG AGAGCTTTGT AAATACTTTG AACCTGGAAA TTTTGTGAAG 
GTTGTGTCTG GCATCCATGA AGGAGGAACT GGCATGATTG TCAAAGTTGA TCAGCATATG 
CTTATCATTC TGTCTGATAC AACCAAAGAA CATATTTGCG TCTTCGCTGA TCATGTTGCC 
AAGAGTGCAG AAGTGACTAA AGGTGTCACA AAAATTGGGG ACTATGAACT TCATGATCTT 
GTGATTCTAA GTGACTTTAG CTTTGGAGTA ATCCTAAAAC TTGATAGTGA AGCTATCCAG 
ATTCTTAAAG GGGTTCCTGA CAGCTCAGAG GTCTCTATTG TCAAAGCCAG CGAAATAAAA 
TACAAAATTT GGAAGAAAAT CAATGTGCAA GACCGTTACA AAAATGTCGT CGCGGTGAAA 
GATGTTGTCA GGGTCATTGA GGGTCCTAGC AAAGGTAAAC AAGGTCCAGT GGTGCAAATC 
TACAAAGGAG TGTTGTTTAT ACATGACCGG CATAACCTTG AACACACTGG ATTTATCTGC 
ACTAGATGCT CATCTTGTGT TCTTGCTGGT GGCAATTTCA AGACACCAGC CCTGGTTCCT 
CCATCTCCAA GGAGATTCCA ACGGGCAGAC ATGGGATATA ACCCTGGAGC TGGAGGGAGA 
CATCAGGGTG GACGAGGGAG AAGGGGTGAC GATCATTTGG TAGGTACTTA TGTCAAAATT 
CGTCTGGGGC CTTTCAAGGG ATATAGTGGG CGTTTAGTTG AAGTCAAAGA TAAGCTAGTG 
CGTGTGGAAC TTGAGGCCAA GATTGTGACA GTTGAGCGGA AGGCGATATC AGATATGACT 
GATAATGTAG TCGCAACCCC GCAGTATAAT ATGGGAAGCC AAACTCCTAT GCATCCTTCC 
CGGACTCCAC TTCATCCCTG TATGACTCCA ATGCGGCATT CTGGAGCTAC ACCTATCCAT 
GATGGAATGA GGACACCTAT GCGTGGTAGA GCTTGGAATC CTTACATGCC TATGAGTCCT 
CCTAGGGATA ACTGGGAAGA TGGGAACCCG GGATCATGGG GAACAAGTCC ATATGAAGCA 
GCTACGCCTG GATCAGATTG GGGTAGTAGT ACTCCTGGTC GTAGTAGTTA CAGAGATGCC 
GGAACACCAA TAAACAATGC TAATGCCCCA AGTCCTATGA CACCAAGCTC AACTTCCTAC 
TTACCTACCA CACCCGGAGG ACAAGCAATG ACTCCTGGAA CCGATCTCGA TGTCATGTCT 
CTTGATATAG GTGGAGATGC AGAAACCAGG TTCATACCCG GTATATTGGT CAATGTACAC 
AAAGCTGGAG AGGATAGAAA TCCCGGTGTT ATTCGAGATG TTCTTCCGGA TGGCTCCTGT 
GTAGTCGCAC TTGGACACAG AGGTGAAGGC GAGACGATAA GAGCGACACA GAACAAAGTG 
AGCCTAGTTT GTCCGAAGAA GAACGAACGT GTGAAGATTC TTGGAGGCAA GTATTGTGGT 
TCAACAGCTA AGGTCATTGG TGAGGACGGT CAGGACGGTA TAGTTAAGCT AGATGAAAGT 
CTCGATATCA AGATATTGAA GCTGACTATA TTAGCCAAGT TAGTTCATGA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC