ZWI cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZWI
Entrez Gene ID	836723
Full Name	kinesin-like calmodulin-binding protein (ZWICHEL)
Synonyms	K14B20.10,K14B20_10,KCBP,KINESIN-LIKE CALMODULIN-BINDING PROTEIN,PKCBP,POTATO KINESIN-LIKE CALMODULIN-BINDING PROTEIN,ZWICHEL
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_125990.4	NP_569022.2	kinesin-like calmodulin-binding protein (ZWICHEL)
NM_180945.2	NP_851276.1	kinesin-like calmodulin-binding protein (ZWICHEL)
NM_001203699.1	NP_001190628.1	kinesin-like calmodulin-binding protein (ZWICHEL)

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:dkey-96l17.6	XP_695803.5
Arabidopsis thaliana (thale cress)	ZWI	NP_001190628.1
Oryza sativa (rice)	Os04g0666900	NP_001054182.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings define a novel genetic and molecular mechanism by which TCS1 interacts with KCBP to regulate trichome cell shape by influencing the stability of microtubules.
Title: TCS1, a Microtubule-Binding Protein, Interacts with KCBP/ZWICHEL to Regulate Trichome Cell Shape in Arabidopsis thaliana.

The MyTH4 domain and the FERM domain in the N-terminal tail of KCBP physically bind to microtubules and F-actin, respectively.
Title: Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin.

The following ZWI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZWI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb22563	NM_180945.2 Latest version!	Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb22564	NM_125990.4 Latest version!	Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb32521	NM_001203699.1 Latest version!	Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZWI ( NM_180945.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_851276.1 Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.

Price & Availability↑

CloneID	OAb22563
Clone ID Related Accession (Same CDS sequence)	NM_180945.2
Accession Version	NM_180945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	kinesin-like calmodulin-binding protein (ZWICHEL)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On May 26, 2011 this sequence version replaced NM_180945.1.

ATGGAGGGCC AACGAGGCAG TAATTCTTCG TTGAGCTCTG GCAATGGCAC CGAAGTCGCC 
ACCGACGTTT CTTCTTGCTT CTATGTTCCC AATCCCTCTG GAACCGATTT CGATGCCGAG 
TCGTCTTCTC TTCCTCCTCT CTCCCCAGCT CCTCAAGTGG CATTGTCAAT TCCTGCGGAG 
CTTGCCGCCG CCATTCCCCT CATCGATCGC TTCCAGGTTG AAGCTTTTCT GCGGCTAATG 
CAGAAACAAA TCCAGTCTGC TGGGAAGCGT GGCTTCTTCT ATTCCAAAAA GTCCTCTGGC 
TCCAATGTCC GCGAGCGCTT CACATTTGAG GATATGCTTT GCTTTCAAAA GGATCCAATC 
CCCACATCTC TCCTCAAGAT TAACAGCGAT CTCGTCAGCC GTGCTACCAA GTTGTTTCAT 
CTCATCTTAA AATATATGGG TGTTGATTCA TCTGATCGAT CTACGCCTCC CAGTTTAGAT 
GAACGCATTG ACCTCGTTGG AAAGCTCTTC AAAAAAACTT TGAAGCGTGT TGAACTCAGG 
GACGAACTTT TTGCCCAAAT CTCCAAACAG ACTAGACATA ATCCTGACAG GCAATACTTG 
ATCAAAGCTT GGGAATTGAT GTACTTATGT GCCTCCTCTA TGCCTCCTAG CAAAGATATC 
GGTGGATATC TATCTGAGTA TATTCATAAT GTCGCACACG ATGCAACTAT TGAACCGGAT 
GCTCAGGTTC TTGCTGTTAA CACTTTGAAA GCTTTAAAGC GCTCTATCAA AGCTGGTCCT 
AGGCACACCA CACCTGGTCG TGAAGAAATT GAAGCCCTTT TGACCGGTAG AAAGCTCACA 
ACCATTGTCT TCTTTCTCGA TGAAACTTTT GAAGAAATTT CATATGACAT GGCTACCACA 
GTGTCTGATG CTGTTGAGCT AGCTGGAACA ATTAAACTAT CAGCTTTCTC TAGCTTTAGT 
TTGTTTGAAT GTCGTAAAGT TGTTTCAAGT TCTAAATCAT CTGATCCCGG AAATGAGGAA 
TATATAGGAT TGGATGATAA CAAGTATATT GGAGATCTCC TCGCAGAATT TAAAGCTATT 
AAAGATCGAA ATAAAGGAGA GATACTACAC TGCAAACTGG TATTTAAAAA AAAATTATTC 
CGAGAGTCTG ATGAAGCTGT AACAGATCTG ATGTTTGTGC AACTTTCGTA TGTTCAACTG 
CAACATGACT ATTTGCTAGG AAACTATCCT GTTGGGAGGG ACGATGCTGC ACAGCTTTGT 
GCCTTGCAAA TTCTTGTTGG GATTGGGTTT GTCAATAGTC CAGAGTCATG CATTGATTGG 
ACATCACTTC TTGAGCGGTT TTTGCCAAGA CAAATAGCAA TAACCCGAGC AAAGCGTGAA 
TGGGAATTGG ATATCCTTGC TCGCTACCGT TCAATGGAGA ACGTGACCAA AGATGATGCA 
AGACAACAAT TTCTACGGAT ACTGAAGGCA CTGCCATACG GGAATTCAGT TTTTTTTAGC 
GTACGCAAGA TAGATGATCC GATCGGTCTT TTACCTGGGC GAATCATTTT GGGTATCAAC 
AAACGTGGGG TTCACTTTTT TCGACCGGTT CCTAAAGAAT ATCTGCACTC TGCTGAACTA 
CGTGACATCA TGCAATTTGG CAGCAGTAAC ACTGCTGTCT TTTTCAAAAT GAGAGTCGCT 
GGTGTTCTTC ACATATTTCA GTTCGAGACA AAACAGGGAG AAGAAATTTG TGTTGCTTTG 
CAAACACATA TAAATGATGT CATGTTGCGT CGTTACTCCA AAGCTCGATC TGCTGCCAAT 
AGCTTGGTTA ATGGAGATAT ATCTTGTAGT TCTAAGCCGC AAAATTTTGA AGTGTATGAA 
AAACGTTTGC AAGATTTGTC TAAGGCTTAT GAAGAGTCCC AAAAGAAGAT TGAGAAGTTG 
ATGGATGAAC AACAAGAGAA AAATCAGCAA GAAGTTACTC TGCGTGAAGA GTTAGAAGCT 
ATACACAATG GTTTGGAGCT TGAAAGGAGA AAATTGTTGG AGGTTACTTT AGACCGAGAT 
AAACTTAGGT CATTGTGTGA CGAGAAGGGA ACAACTATTC AATCCTTGAT GTCTGAACTG 
CGAGGAATGG AAGCAAGGTT GGCAAAGTCG GGCAACACAA AATCAAGTAA AGAGACAAAA 
TCAGAATTAG CCGAAATGAA TAATCAGATA TTATACAAGA TCCAAAAGGA GTTAGAAGTT 
CGAAATAAGG AATTGCATGT CGCAGTTGAT AATTCAAAGA GGTTGTTGAG TGAAAACAAG 
ATATTGGAGC AAAATCTCAA TATTGAAAAG AAGAAAAAAG AGGAGGTTGA AATTCATCAA 
AAGAGATATG AACAAGAAAA AAAGGTGTTA AAGCTTCGAG TTTCTGAACT TGAAAATAAG 
CTTGAAGTAC TTGCTCAAGA CTTGGATAGT GCTGAGTCTA CGATTGAAAG TAAGAATTCT 
GACATGCTGC TGTTGCAAAA TAACTTGAAA GAACTTGAGG AGTTAAGAGA AATGAAAGAG 
GACATTGACA GAAAAAATGA GCAAACAGCT GCCATTTTGA AAATGCAAGG AGCCCAACTT 
GCTGAGCTAG AAATACTTTA TAAGGAAGAA CAAGTTTTAA GGAAAAGATA CTATAATACC 
ATAGAAGATA TGAAAGGGAA GATTAGAGTT TATTGTCGAA TAAGACCTCT AAATGAAAAA 
GAGAGTTCAG AGAGGGAAAA ACAAATGCTG ACAACTGTGG ATGAGTTTAC TGTTGAACAT 
CCATGGAAAG ACGACAAAAG AAAGCAACAC ATATATGATC GCGTATTTGA CATGCGTGCT 
AGTCAAGATG ATATCTTTGA AGACACAAAG TATTTGGTAC AGTCGGCTGT AGATGGGTAT 
AACGTTTGCA TCTTTGCATA TGGTCAAACT GGTTCTGGAA AAACTTTCAC TATATATGGG 
CATGAGAGCA ATCCTGGACT CACACCTCGA GCTACAAAGG AACTGTTCAA CATATTAAAG 
CGAGATAGCA AGAGATTTTC ATTTTCTCTA AAGGCATATA TGGTGGAACT TTATCAAGAC 
ACACTTGTAG ACCTTTTGTT ACCAAAAAGT GCAAGACGCT TGAAACTAGA GATTAAAAAA 
GATTCAAAGG GAATGGTCTT TGTAGAGAAT GTGACAACTA TTCCTATATC AACTTTGGAG 
GAACTGCGAA TGATTCTTGA ACGGGGATCG GAACGACGAC ATGTTTCTGG AACAAATATG 
AATGAAGAAA GCTCAAGATC TCACCTCATA TTATCAGTTG TTATTGAAAG TATTGATCTT 
CAAACCCAGT CTGCTGCGAG GGGCAAGCTG AGTTTTGTGG ATCTTGCTGG TTCTGAGAGA 
GTTAAAAAGT CGGGCTCAGC TGGTTGTCAA CTCAAAGAAG CTCAAAGTAT CAACAAATCA 
CTTTCTGCAT TAGGTGATGT TATTGGTGCT TTATCTTCTG GCAACCAGCA TATTCCTTAT 
AGGAATCACA AGCTAACGAT GTTGATGAGC GATTCATTGG GCGGCAATGC CAAGACGTTA 
ATGTTTGTTA ATGTGTCTCC AGCCGAATCA AATTTGGACG AGACGTACAA TTCTCTTCTA 
TATGCATCGA GAGTGAGAAC GATCGTGAAT GATCCCAGCA AACATATATC ATCCAAAGAG 
ATGGTGCGAT TGAAGAAGTT GGTAGCATAC TGGAAAGAGC AAGCCGGTAA AAAAGGTGAG 
GAAGAAGACT TGGTGGATAT TGAGGAAGAT CGTACACGAA AAGATGAGGC AGATAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851276.1
CDS	127..3906
Translation	MEGQRGSNSSLSSGNGTEVATDVSSCFYVPNPSGTDFDAESSSLPPLSPAPQVALSIPAELAAAIPLIDRFQVEAFLRLMQKQIQSAGKRGFFYSKKSSGSNVRERFTFEDMLCFQKDPIPTSLLKINSDLVSRATKLFHLILKYMGVDSSDRSTPPSLDERIDLVGKLFKKTLKRVELRDELFAQISKQTRHNPDRQYLIKAWELMYLCASSMPPSKDIGGYLSEYIHNVAHDATIEPDAQVLAVNTLKALKRSIKAGPRHTTPGREEIEALLTGRKLTTIVFFLDETFEEISYDMATTVSDAVELAGTIKLSAFSSFSLFECRKVVSSSKSSDPGNEEYIGLDDNKYIGDLLAEFKAIKDRNKGEILHCKLVFKKKLFRESDEAVTDLMFVQLSYVQLQHDYLLGNYPVGRDDAAQLCALQILVGIGFVNSPESCIDWTSLLERFLPRQIAITRAKREWELDILARYRSMENVTKDDARQQFLRILKALPYGNSVFFSVRKIDDPIGLLPGRIILGINKRGVHFFRPVPKEYLHSAELRDIMQFGSSNTAVFFKMRVAGVLHIFQFETKQGEEICVALQTHINDVMLRRYSKARSAANSLVNGDISCSSKPQNFEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKNQQEVTLREELEAIHNGLELERRKLLEVTLDRDKLRSLCDEKGTTIQSLMSELRGMEARLAKSGNTKSSKETKSELAEMNNQILYKIQKELEVRNKELHVAVDNSKRLLSENKILEQNLNIEKKKKEEVEIHQKRYEQEKKVLKLRVSELENKLEVLAQDLDSAESTIESKNSDMLLLQNNLKELEELREMKEDIDRKNEQTAAILKMQGAQLAELEILYKEEQVLRKRYYNTIEDMKGKIRVYCRIRPLNEKESSEREKQMLTTVDEFTVEHPWKDDKRKQHIYDRVFDMRASQDDIFEDTKYLVQSAVDGYNVCIFAYGQTGSGKTFTIYGHESNPGLTPRATKELFNILKRDSKRFSFSLKAYMVELYQDTLVDLLLPKSARRLKLEIKKDSKGMVFVENVTTIPISTLEELRMILERGSERRHVSGTNMNEESSRSHLILSVVIESIDLQTQSAARGKLSFVDLAGSERVKKSGSAGCQLKEAQSINKSLSALGDVIGALSSGNQHIPYRNHKLTMLMSDSLGGNAKTLMFVNVSPAESNLDETYNSLLYASRVRTIVNDPSKHISSKEMVRLKKLVAYWKEQAGKKGEEEDLVDIEEDRTRKDEADS

Target ORF information:

RefSeq Version	NM_180945.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180945.2

ORF Insert Sequence:

ATGGAGGGCC AACGAGGCAG TAATTCTTCG TTGAGCTCTG GCAATGGCAC CGAAGTCGCC 
ACCGACGTTT CTTCTTGCTT CTATGTTCCC AATCCCTCTG GAACCGATTT CGATGCCGAG 
TCGTCTTCTC TTCCTCCTCT CTCCCCAGCT CCTCAAGTGG CATTGTCAAT TCCTGCGGAG 
CTTGCCGCCG CCATTCCCCT CATCGATCGC TTCCAGGTTG AAGCTTTTCT GCGGCTAATG 
CAGAAACAAA TCCAGTCTGC TGGGAAGCGT GGCTTCTTCT ATTCCAAAAA GTCCTCTGGC 
TCCAATGTCC GCGAGCGCTT CACATTTGAG GATATGCTTT GCTTTCAAAA GGATCCAATC 
CCCACATCTC TCCTCAAGAT TAACAGCGAT CTCGTCAGCC GTGCTACCAA GTTGTTTCAT 
CTCATCTTAA AATATATGGG TGTTGATTCA TCTGATCGAT CTACGCCTCC CAGTTTAGAT 
GAACGCATTG ACCTCGTTGG AAAGCTCTTC AAAAAAACTT TGAAGCGTGT TGAACTCAGG 
GACGAACTTT TTGCCCAAAT CTCCAAACAG ACTAGACATA ATCCTGACAG GCAATACTTG 
ATCAAAGCTT GGGAATTGAT GTACTTATGT GCCTCCTCTA TGCCTCCTAG CAAAGATATC 
GGTGGATATC TATCTGAGTA TATTCATAAT GTCGCACACG ATGCAACTAT TGAACCGGAT 
GCTCAGGTTC TTGCTGTTAA CACTTTGAAA GCTTTAAAGC GCTCTATCAA AGCTGGTCCT 
AGGCACACCA CACCTGGTCG TGAAGAAATT GAAGCCCTTT TGACCGGTAG AAAGCTCACA 
ACCATTGTCT TCTTTCTCGA TGAAACTTTT GAAGAAATTT CATATGACAT GGCTACCACA 
GTGTCTGATG CTGTTGAGCT AGCTGGAACA ATTAAACTAT CAGCTTTCTC TAGCTTTAGT 
TTGTTTGAAT GTCGTAAAGT TGTTTCAAGT TCTAAATCAT CTGATCCCGG AAATGAGGAA 
TATATAGGAT TGGATGATAA CAAGTATATT GGAGATCTCC TCGCAGAATT TAAAGCTATT 
AAAGATCGAA ATAAAGGAGA GATACTACAC TGCAAACTGG TATTTAAAAA AAAATTATTC 
CGAGAGTCTG ATGAAGCTGT AACAGATCTG ATGTTTGTGC AACTTTCGTA TGTTCAACTG 
CAACATGACT ATTTGCTAGG AAACTATCCT GTTGGGAGGG ACGATGCTGC ACAGCTTTGT 
GCCTTGCAAA TTCTTGTTGG GATTGGGTTT GTCAATAGTC CAGAGTCATG CATTGATTGG 
ACATCACTTC TTGAGCGGTT TTTGCCAAGA CAAATAGCAA TAACCCGAGC AAAGCGTGAA 
TGGGAATTGG ATATCCTTGC TCGCTACCGT TCAATGGAGA ACGTGACCAA AGATGATGCA 
AGACAACAAT TTCTACGGAT ACTGAAGGCA CTGCCATACG GGAATTCAGT TTTTTTTAGC 
GTACGCAAGA TAGATGATCC GATCGGTCTT TTACCTGGGC GAATCATTTT GGGTATCAAC 
AAACGTGGGG TTCACTTTTT TCGACCGGTT CCTAAAGAAT ATCTGCACTC TGCTGAACTA 
CGTGACATCA TGCAATTTGG CAGCAGTAAC ACTGCTGTCT TTTTCAAAAT GAGAGTCGCT 
GGTGTTCTTC ACATATTTCA GTTCGAGACA AAACAGGGAG AAGAAATTTG TGTTGCTTTG 
CAAACACATA TAAATGATGT CATGTTGCGT CGTTACTCCA AAGCTCGATC TGCTGCCAAT 
AGCTTGGTTA ATGGAGATAT ATCTTGTAGT TCTAAGCCGC AAAATTTTGA AGTGTATGAA 
AAACGTTTGC AAGATTTGTC TAAGGCTTAT GAAGAGTCCC AAAAGAAGAT TGAGAAGTTG 
ATGGATGAAC AACAAGAGAA AAATCAGCAA GAAGTTACTC TGCGTGAAGA GTTAGAAGCT 
ATACACAATG GTTTGGAGCT TGAAAGGAGA AAATTGTTGG AGGTTACTTT AGACCGAGAT 
AAACTTAGGT CATTGTGTGA CGAGAAGGGA ACAACTATTC AATCCTTGAT GTCTGAACTG 
CGAGGAATGG AAGCAAGGTT GGCAAAGTCG GGCAACACAA AATCAAGTAA AGAGACAAAA 
TCAGAATTAG CCGAAATGAA TAATCAGATA TTATACAAGA TCCAAAAGGA GTTAGAAGTT 
CGAAATAAGG AATTGCATGT CGCAGTTGAT AATTCAAAGA GGTTGTTGAG TGAAAACAAG 
ATATTGGAGC AAAATCTCAA TATTGAAAAG AAGAAAAAAG AGGAGGTTGA AATTCATCAA 
AAGAGATATG AACAAGAAAA AAAGGTGTTA AAGCTTCGAG TTTCTGAACT TGAAAATAAG 
CTTGAAGTAC TTGCTCAAGA CTTGGATAGT GCTGAGTCTA CGATTGAAAG TAAGAATTCT 
GACATGCTGC TGTTGCAAAA TAACTTGAAA GAACTTGAGG AGTTAAGAGA AATGAAAGAG 
GACATTGACA GAAAAAATGA GCAAACAGCT GCCATTTTGA AAATGCAAGG AGCCCAACTT 
GCTGAGCTAG AAATACTTTA TAAGGAAGAA CAAGTTTTAA GGAAAAGATA CTATAATACC 
ATAGAAGATA TGAAAGGGAA GATTAGAGTT TATTGTCGAA TAAGACCTCT AAATGAAAAA 
GAGAGTTCAG AGAGGGAAAA ACAAATGCTG ACAACTGTGG ATGAGTTTAC TGTTGAACAT 
CCATGGAAAG ACGACAAAAG AAAGCAACAC ATATATGATC GCGTATTTGA CATGCGTGCT 
AGTCAAGATG ATATCTTTGA AGACACAAAG TATTTGGTAC AGTCGGCTGT AGATGGGTAT 
AACGTTTGCA TCTTTGCATA TGGTCAAACT GGTTCTGGAA AAACTTTCAC TATATATGGG 
CATGAGAGCA ATCCTGGACT CACACCTCGA GCTACAAAGG AACTGTTCAA CATATTAAAG 
CGAGATAGCA AGAGATTTTC ATTTTCTCTA AAGGCATATA TGGTGGAACT TTATCAAGAC 
ACACTTGTAG ACCTTTTGTT ACCAAAAAGT GCAAGACGCT TGAAACTAGA GATTAAAAAA 
GATTCAAAGG GAATGGTCTT TGTAGAGAAT GTGACAACTA TTCCTATATC AACTTTGGAG 
GAACTGCGAA TGATTCTTGA ACGGGGATCG GAACGACGAC ATGTTTCTGG AACAAATATG 
AATGAAGAAA GCTCAAGATC TCACCTCATA TTATCAGTTG TTATTGAAAG TATTGATCTT 
CAAACCCAGT CTGCTGCGAG GGGCAAGCTG AGTTTTGTGG ATCTTGCTGG TTCTGAGAGA 
GTTAAAAAGT CGGGCTCAGC TGGTTGTCAA CTCAAAGAAG CTCAAAGTAT CAACAAATCA 
CTTTCTGCAT TAGGTGATGT TATTGGTGCT TTATCTTCTG GCAACCAGCA TATTCCTTAT 
AGGAATCACA AGCTAACGAT GTTGATGAGC GATTCATTGG GCGGCAATGC CAAGACGTTA 
ATGTTTGTTA ATGTGTCTCC AGCCGAATCA AATTTGGACG AGACGTACAA TTCTCTTCTA 
TATGCATCGA GAGTGAGAAC GATCGTGAAT GATCCCAGCA AACATATATC ATCCAAAGAG 
ATGGTGCGAT TGAAGAAGTT GGTAGCATAC TGGAAAGAGC AAGCCGGTAA AAAAGGTGAG 
GAAGAAGACT TGGTGGATAT TGAGGAAGAT CGTACACGAA AAGATGAGGC AGATAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWI ( NM_125990.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_569022.2 Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.

Price & Availability↑

CloneID	OAb22564
Clone ID Related Accession (Same CDS sequence)	NM_125990.4
Accession Version	NM_125990.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	kinesin-like calmodulin-binding protein (ZWICHEL)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_125990.3.

ATGGAGGGCC AACGAGGCAG TAATTCTTCG TTGAGCTCTG GCAATGGCAC CGAAGTCGCC 
ACCGACGTTT CTTCTTGCTT CTATGTTCCC AATCCCTCTG GAACCGATTT CGATGCCGAG 
TCGTCTTCTC TTCCTCCTCT CTCCCCAGCT CCTCAAGTGG CATTGTCAAT TCCTGCGGAG 
CTTGCCGCCG CCATTCCCCT CATCGATCGC TTCCAGGTTG AAGCTTTTCT GCGGCTAATG 
CAGAAACAAA TCCAGTCTGC TGGGAAGCGT GGCTTCTTCT ATTCCAAAAA GTCCTCTGGC 
TCCAATGTCC GCGAGCGCTT CACATTTGAG GATATGCTTT GCTTTCAAAA GGATCCAATC 
CCCACATCTC TCCTCAAGAT TAACAGCGAT CTCGTCAGCC GTGCTACCAA GTTGTTTCAT 
CTCATCTTAA AATATATGGG TGTTGATTCA TCTGATCGAT CTACGCCTCC CAGTTTAGAT 
GAACGCATTG ACCTCGTTGG AAAGCTCTTC AAAAAAACTT TGAAGCGTGT TGAACTCAGG 
GACGAACTTT TTGCCCAAAT CTCCAAACAG ACTAGACATA ATCCTGACAG GCAATACTTG 
ATCAAAGCTT GGGAATTGAT GTACTTATGT GCCTCCTCTA TGCCTCCTAG CAAAGATATC 
GGTGGATATC TATCTGAGTA TATTCATAAT GTCGCACACG ATGCAACTAT TGAACCGGAT 
GCTCAGGTTC TTGCTGTTAA CACTTTGAAA GCTTTAAAGC GCTCTATCAA AGCTGGTCCT 
AGGCACACCA CACCTGGTCG TGAAGAAATT GAAGCCCTTT TGACCGGTAG AAAGCTCACA 
ACCATTGTCT TCTTTCTCGA TGAAACTTTT GAAGAAATTT CATATGACAT GGCTACCACA 
GTGTCTGATG CTGTTGAGGA GCTAGCTGGA ACAATTAAAC TATCAGCTTT CTCTAGCTTT 
AGTTTGTTTG AATGTCGTAA AGTTGTTTCA AGTTCTAAAT CATCTGATCC CGGAAATGAG 
GAATATATAG GATTGGATGA TAACAAGTAT ATTGGAGATC TCCTCGCAGA ATTTAAAGCT 
ATTAAAGATC GAAATAAAGG AGAGATACTA CACTGCAAAC TGGTATTTAA AAAAAAATTA 
TTCCGAGAGT CTGATGAAGC TGTAACAGAT CTGATGTTTG TGCAACTTTC GTATGTTCAA 
CTGCAACATG ACTATTTGCT AGGAAACTAT CCTGTTGGGA GGGACGATGC TGCACAGCTT 
TGTGCCTTGC AAATTCTTGT TGGGATTGGG TTTGTCAATA GTCCAGAGTC ATGCATTGAT 
TGGACATCAC TTCTTGAGCG GTTTTTGCCA AGACAAATAG CAATAACCCG AGCAAAGCGT 
GAATGGGAAT TGGATATCCT TGCTCGCTAC CGTTCAATGG AGAACGTGAC CAAAGATGAT 
GCAAGACAAC AATTTCTACG GATACTGAAG GCACTGCCAT ACGGGAATTC AGTTTTTTTT 
AGCGTACGCA AGATAGATGA TCCGATCGGT CTTTTACCTG GGCGAATCAT TTTGGGTATC 
AACAAACGTG GGGTTCACTT TTTTCGACCG GTTCCTAAAG AATATCTGCA CTCTGCTGAA 
CTACGTGACA TCATGCAATT TGGCAGCAGT AACACTGCTG TCTTTTTCAA AATGAGAGTC 
GCTGGTGTTC TTCACATATT TCAGTTCGAG ACAAAACAGG GAGAAGAAAT TTGTGTTGCT 
TTGCAAACAC ATATAAATGA TGTCATGTTG CGTCGTTACT CCAAAGCTCG ATCTGCTGCC 
AATAGCTTGG TTAATGGAGA TATATCTTGT AGTTCTAAGC CGCAAAATTT TGAAGTGTAT 
GAAAAACGTT TGCAAGATTT GTCTAAGGCT TATGAAGAGT CCCAAAAGAA GATTGAGAAG 
TTGATGGATG AACAACAAGA GAAAAATCAG CAAGAAGTTA CTCTGCGTGA AGAGTTAGAA 
GCTATACACA ATGGTTTGGA GCTTGAAAGG AGAAAATTGT TGGAGGTTAC TTTAGACCGA 
GATAAACTTA GGTCATTGTG TGACGAGAAG GGAACAACTA TTCAATCCTT GATGTCTGAA 
CTGCGAGGAA TGGAAGCAAG GTTGGCAAAG TCGGGCAACA CAAAATCAAG TAAAGAGACA 
AAATCAGAAT TAGCCGAAAT GAATAATCAG ATATTATACA AGATCCAAAA GGAGTTAGAA 
GTTCGAAATA AGGAATTGCA TGTCGCAGTT GATAATTCAA AGAGGTTGTT GAGTGAAAAC 
AAGATATTGG AGCAAAATCT CAATATTGAA AAGAAGAAAA AAGAGGAGGT TGAAATTCAT 
CAAAAGAGAT ATGAACAAGA AAAAAAGGTG TTAAAGCTTC GAGTTTCTGA ACTTGAAAAT 
AAGCTTGAAG TACTTGCTCA AGACTTGGAT AGTGCTGAGT CTACGATTGA AAGTAAGAAT 
TCTGACATGC TGCTGTTGCA AAATAACTTG AAAGAACTTG AGGAGTTAAG AGAAATGAAA 
GAGGACATTG ACAGAAAAAA TGAGCAAACA GCTGCCATTT TGAAAATGCA AGGAGCCCAA 
CTTGCTGAGC TAGAAATACT TTATAAGGAA GAACAAGTTT TAAGGAAAAG ATACTATAAT 
ACCATAGAAG ATATGAAAGG GAAGATTAGA GTTTATTGTC GAATAAGACC TCTAAATGAA 
AAAGAGAGTT CAGAGAGGGA AAAACAAATG CTGACAACTG TGGATGAGTT TACTGTTGAA 
CATCCATGGA AAGACGACAA AAGAAAGCAA CACATATATG ATCGCGTATT TGACATGCGT 
GCTAGTCAAG ATGATATCTT TGAAGACACA AAGTATTTGG TACAGTCGGC TGTAGATGGG 
TATAACGTTT GCATCTTTGC ATATGGTCAA ACTGGTTCTG GAAAAACTTT CACTATATAT 
GGGCATGAGA GCAATCCTGG ACTCACACCT CGAGCTACAA AGGAACTGTT CAACATATTA 
AAGCGAGATA GCAAGAGATT TTCATTTTCT CTAAAGGCAT ATATGGTGGA ACTTTATCAA 
GACACACTTG TAGACCTTTT GTTACCAAAA AGTGCAAGAC GCTTGAAACT AGAGATTAAA 
AAAGATTCAA AGGGAATGGT CTTTGTAGAG AATGTGACAA CTATTCCTAT ATCAACTTTG 
GAGGAACTGC GAATGATTCT TGAACGGGGA TCGGAACGAC GACATGTTTC TGGAACAAAT 
ATGAATGAAG AAAGCTCAAG ATCTCACCTC ATATTATCAG TTGTTATTGA AAGTATTGAT 
CTTCAAACCC AGTCTGCTGC GAGGGGCAAG CTGAGTTTTG TGGATCTTGC TGGTTCTGAG 
AGAGTTAAAA AGTCGGGCTC AGCTGGTTGT CAACTCAAAG AAGCTCAAAG TATCAACAAA 
TCACTTTCTG CATTAGGTGA TGTTATTGGT GCTTTATCTT CTGGCAACCA GCATATTCCT 
TATAGGAATC ACAAGCTAAC GATGTTGATG AGCGATTCAT TGGGCGGCAA TGCCAAGACG 
TTAATGTTTG TTAATGTGTC TCCAGCCGAA TCAAATTTGG ACGAGACGTA CAATTCTCTT 
CTATATGCAT CGAGAGTGAG AACGATCGTG AATGATCCCA GCAAACATAT ATCATCCAAA 
GAGATGGTGC GATTGAAGAA GTTGGTAGCA TACTGGAAAG AGCAAGCCGG TAAAAAAGGT 
GAGGAAGAAG ACTTGGTGGA TATTGAGGAA GATCGTACAC GAAAAGATGA GGCAGATAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569022.2
CDS	179..3961
Translation	MEGQRGSNSSLSSGNGTEVATDVSSCFYVPNPSGTDFDAESSSLPPLSPAPQVALSIPAELAAAIPLIDRFQVEAFLRLMQKQIQSAGKRGFFYSKKSSGSNVRERFTFEDMLCFQKDPIPTSLLKINSDLVSRATKLFHLILKYMGVDSSDRSTPPSLDERIDLVGKLFKKTLKRVELRDELFAQISKQTRHNPDRQYLIKAWELMYLCASSMPPSKDIGGYLSEYIHNVAHDATIEPDAQVLAVNTLKALKRSIKAGPRHTTPGREEIEALLTGRKLTTIVFFLDETFEEISYDMATTVSDAVEELAGTIKLSAFSSFSLFECRKVVSSSKSSDPGNEEYIGLDDNKYIGDLLAEFKAIKDRNKGEILHCKLVFKKKLFRESDEAVTDLMFVQLSYVQLQHDYLLGNYPVGRDDAAQLCALQILVGIGFVNSPESCIDWTSLLERFLPRQIAITRAKREWELDILARYRSMENVTKDDARQQFLRILKALPYGNSVFFSVRKIDDPIGLLPGRIILGINKRGVHFFRPVPKEYLHSAELRDIMQFGSSNTAVFFKMRVAGVLHIFQFETKQGEEICVALQTHINDVMLRRYSKARSAANSLVNGDISCSSKPQNFEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKNQQEVTLREELEAIHNGLELERRKLLEVTLDRDKLRSLCDEKGTTIQSLMSELRGMEARLAKSGNTKSSKETKSELAEMNNQILYKIQKELEVRNKELHVAVDNSKRLLSENKILEQNLNIEKKKKEEVEIHQKRYEQEKKVLKLRVSELENKLEVLAQDLDSAESTIESKNSDMLLLQNNLKELEELREMKEDIDRKNEQTAAILKMQGAQLAELEILYKEEQVLRKRYYNTIEDMKGKIRVYCRIRPLNEKESSEREKQMLTTVDEFTVEHPWKDDKRKQHIYDRVFDMRASQDDIFEDTKYLVQSAVDGYNVCIFAYGQTGSGKTFTIYGHESNPGLTPRATKELFNILKRDSKRFSFSLKAYMVELYQDTLVDLLLPKSARRLKLEIKKDSKGMVFVENVTTIPISTLEELRMILERGSERRHVSGTNMNEESSRSHLILSVVIESIDLQTQSAARGKLSFVDLAGSERVKKSGSAGCQLKEAQSINKSLSALGDVIGALSSGNQHIPYRNHKLTMLMSDSLGGNAKTLMFVNVSPAESNLDETYNSLLYASRVRTIVNDPSKHISSKEMVRLKKLVAYWKEQAGKKGEEEDLVDIEEDRTRKDEADS

Target ORF information:

RefSeq Version	NM_125990.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125990.4

ORF Insert Sequence:

ATGGAGGGCC AACGAGGCAG TAATTCTTCG TTGAGCTCTG GCAATGGCAC CGAAGTCGCC 
ACCGACGTTT CTTCTTGCTT CTATGTTCCC AATCCCTCTG GAACCGATTT CGATGCCGAG 
TCGTCTTCTC TTCCTCCTCT CTCCCCAGCT CCTCAAGTGG CATTGTCAAT TCCTGCGGAG 
CTTGCCGCCG CCATTCCCCT CATCGATCGC TTCCAGGTTG AAGCTTTTCT GCGGCTAATG 
CAGAAACAAA TCCAGTCTGC TGGGAAGCGT GGCTTCTTCT ATTCCAAAAA GTCCTCTGGC 
TCCAATGTCC GCGAGCGCTT CACATTTGAG GATATGCTTT GCTTTCAAAA GGATCCAATC 
CCCACATCTC TCCTCAAGAT TAACAGCGAT CTCGTCAGCC GTGCTACCAA GTTGTTTCAT 
CTCATCTTAA AATATATGGG TGTTGATTCA TCTGATCGAT CTACGCCTCC CAGTTTAGAT 
GAACGCATTG ACCTCGTTGG AAAGCTCTTC AAAAAAACTT TGAAGCGTGT TGAACTCAGG 
GACGAACTTT TTGCCCAAAT CTCCAAACAG ACTAGACATA ATCCTGACAG GCAATACTTG 
ATCAAAGCTT GGGAATTGAT GTACTTATGT GCCTCCTCTA TGCCTCCTAG CAAAGATATC 
GGTGGATATC TATCTGAGTA TATTCATAAT GTCGCACACG ATGCAACTAT TGAACCGGAT 
GCTCAGGTTC TTGCTGTTAA CACTTTGAAA GCTTTAAAGC GCTCTATCAA AGCTGGTCCT 
AGGCACACCA CACCTGGTCG TGAAGAAATT GAAGCCCTTT TGACCGGTAG AAAGCTCACA 
ACCATTGTCT TCTTTCTCGA TGAAACTTTT GAAGAAATTT CATATGACAT GGCTACCACA 
GTGTCTGATG CTGTTGAGGA GCTAGCTGGA ACAATTAAAC TATCAGCTTT CTCTAGCTTT 
AGTTTGTTTG AATGTCGTAA AGTTGTTTCA AGTTCTAAAT CATCTGATCC CGGAAATGAG 
GAATATATAG GATTGGATGA TAACAAGTAT ATTGGAGATC TCCTCGCAGA ATTTAAAGCT 
ATTAAAGATC GAAATAAAGG AGAGATACTA CACTGCAAAC TGGTATTTAA AAAAAAATTA 
TTCCGAGAGT CTGATGAAGC TGTAACAGAT CTGATGTTTG TGCAACTTTC GTATGTTCAA 
CTGCAACATG ACTATTTGCT AGGAAACTAT CCTGTTGGGA GGGACGATGC TGCACAGCTT 
TGTGCCTTGC AAATTCTTGT TGGGATTGGG TTTGTCAATA GTCCAGAGTC ATGCATTGAT 
TGGACATCAC TTCTTGAGCG GTTTTTGCCA AGACAAATAG CAATAACCCG AGCAAAGCGT 
GAATGGGAAT TGGATATCCT TGCTCGCTAC CGTTCAATGG AGAACGTGAC CAAAGATGAT 
GCAAGACAAC AATTTCTACG GATACTGAAG GCACTGCCAT ACGGGAATTC AGTTTTTTTT 
AGCGTACGCA AGATAGATGA TCCGATCGGT CTTTTACCTG GGCGAATCAT TTTGGGTATC 
AACAAACGTG GGGTTCACTT TTTTCGACCG GTTCCTAAAG AATATCTGCA CTCTGCTGAA 
CTACGTGACA TCATGCAATT TGGCAGCAGT AACACTGCTG TCTTTTTCAA AATGAGAGTC 
GCTGGTGTTC TTCACATATT TCAGTTCGAG ACAAAACAGG GAGAAGAAAT TTGTGTTGCT 
TTGCAAACAC ATATAAATGA TGTCATGTTG CGTCGTTACT CCAAAGCTCG ATCTGCTGCC 
AATAGCTTGG TTAATGGAGA TATATCTTGT AGTTCTAAGC CGCAAAATTT TGAAGTGTAT 
GAAAAACGTT TGCAAGATTT GTCTAAGGCT TATGAAGAGT CCCAAAAGAA GATTGAGAAG 
TTGATGGATG AACAACAAGA GAAAAATCAG CAAGAAGTTA CTCTGCGTGA AGAGTTAGAA 
GCTATACACA ATGGTTTGGA GCTTGAAAGG AGAAAATTGT TGGAGGTTAC TTTAGACCGA 
GATAAACTTA GGTCATTGTG TGACGAGAAG GGAACAACTA TTCAATCCTT GATGTCTGAA 
CTGCGAGGAA TGGAAGCAAG GTTGGCAAAG TCGGGCAACA CAAAATCAAG TAAAGAGACA 
AAATCAGAAT TAGCCGAAAT GAATAATCAG ATATTATACA AGATCCAAAA GGAGTTAGAA 
GTTCGAAATA AGGAATTGCA TGTCGCAGTT GATAATTCAA AGAGGTTGTT GAGTGAAAAC 
AAGATATTGG AGCAAAATCT CAATATTGAA AAGAAGAAAA AAGAGGAGGT TGAAATTCAT 
CAAAAGAGAT ATGAACAAGA AAAAAAGGTG TTAAAGCTTC GAGTTTCTGA ACTTGAAAAT 
AAGCTTGAAG TACTTGCTCA AGACTTGGAT AGTGCTGAGT CTACGATTGA AAGTAAGAAT 
TCTGACATGC TGCTGTTGCA AAATAACTTG AAAGAACTTG AGGAGTTAAG AGAAATGAAA 
GAGGACATTG ACAGAAAAAA TGAGCAAACA GCTGCCATTT TGAAAATGCA AGGAGCCCAA 
CTTGCTGAGC TAGAAATACT TTATAAGGAA GAACAAGTTT TAAGGAAAAG ATACTATAAT 
ACCATAGAAG ATATGAAAGG GAAGATTAGA GTTTATTGTC GAATAAGACC TCTAAATGAA 
AAAGAGAGTT CAGAGAGGGA AAAACAAATG CTGACAACTG TGGATGAGTT TACTGTTGAA 
CATCCATGGA AAGACGACAA AAGAAAGCAA CACATATATG ATCGCGTATT TGACATGCGT 
GCTAGTCAAG ATGATATCTT TGAAGACACA AAGTATTTGG TACAGTCGGC TGTAGATGGG 
TATAACGTTT GCATCTTTGC ATATGGTCAA ACTGGTTCTG GAAAAACTTT CACTATATAT 
GGGCATGAGA GCAATCCTGG ACTCACACCT CGAGCTACAA AGGAACTGTT CAACATATTA 
AAGCGAGATA GCAAGAGATT TTCATTTTCT CTAAAGGCAT ATATGGTGGA ACTTTATCAA 
GACACACTTG TAGACCTTTT GTTACCAAAA AGTGCAAGAC GCTTGAAACT AGAGATTAAA 
AAAGATTCAA AGGGAATGGT CTTTGTAGAG AATGTGACAA CTATTCCTAT ATCAACTTTG 
GAGGAACTGC GAATGATTCT TGAACGGGGA TCGGAACGAC GACATGTTTC TGGAACAAAT 
ATGAATGAAG AAAGCTCAAG ATCTCACCTC ATATTATCAG TTGTTATTGA AAGTATTGAT 
CTTCAAACCC AGTCTGCTGC GAGGGGCAAG CTGAGTTTTG TGGATCTTGC TGGTTCTGAG 
AGAGTTAAAA AGTCGGGCTC AGCTGGTTGT CAACTCAAAG AAGCTCAAAG TATCAACAAA 
TCACTTTCTG CATTAGGTGA TGTTATTGGT GCTTTATCTT CTGGCAACCA GCATATTCCT 
TATAGGAATC ACAAGCTAAC GATGTTGATG AGCGATTCAT TGGGCGGCAA TGCCAAGACG 
TTAATGTTTG TTAATGTGTC TCCAGCCGAA TCAAATTTGG ACGAGACGTA CAATTCTCTT 
CTATATGCAT CGAGAGTGAG AACGATCGTG AATGATCCCA GCAAACATAT ATCATCCAAA 
GAGATGGTGC GATTGAAGAA GTTGGTAGCA TACTGGAAAG AGCAAGCCGG TAAAAAAGGT 
GAGGAAGAAG ACTTGGTGGA TATTGAGGAA GATCGTACAC GAAAAGATGA GGCAGATAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWI ( NM_001203699.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190628.1 Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.

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CloneID	OAb32521
Clone ID Related Accession (Same CDS sequence)	NM_001203699.1
Accession Version	NM_001203699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	kinesin-like calmodulin-binding protein (ZWICHEL)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGAGGGCC AACGAGGCAG TAATTCTTCG TTGAGCTCTG GCAATGGCAC CGAAGTCGCC 
ACCGACGTTT CTTCTTGCTT CTATGTTCCC AATCCCTCTG GAACCGATTT CGATGCCGAG 
TCGTCTTCTC TTCCTCCTCT TCTCTCGCTC TGTCACAGCT CCCCAGCTCC TCAAGTGGCA 
TTGTCAATTC CTGCGGAGCT TGCCGCCGCC ATTCCCCTCA TCGATCGCTT CCAGGTTGAA 
GCTTTTCTGC GGCTAATGCA GAAACAAATC CAGTCTGCTG GGAAGCGTGG CTTCTTCTAT 
TCCAAAAAGT CCTCTGGCTC CAATGTCCGC GAGCGCTTCA CATTTGAGGA TATGCTTTGC 
TTTCAAAAGG ATCCAATCCC CACATCTCTC CTCAAGATTA ACAGCGATCT CGTCAGCCGT 
GCTACCAAGT TGTTTCATCT CATCTTAAAA TATATGGGTG TTGATTCATC TGATCGATCT 
ACGCCTCCCA GTTTAGATGA ACGCATTGAC CTCGTTGGAA AGCTCTTCAA AAAAACTTTG 
AAGCGTGTTG AACTCAGGGA CGAACTTTTT GCCCAAATCT CCAAACAGAC TAGACATAAT 
CCTGACAGGC AATACTTGAT CAAAGCTTGG GAATTGATGT ACTTATGTGC CTCCTCTATG 
CCTCCTAGCA AAGATATCGG TGGATATCTA TCTGAGTATA TTCATAATGT CGCACACGAT 
GCAACTATTG AACCGGATGC TCAGGTTCTT GCTGTTAACA CTTTGAAAGC TTTAAAGCGC 
TCTATCAAAG CTGGTCCTAG GCACACCACA CCTGGTCGTG AAGAAATTGA AGCCCTTTTG 
ACCGGTAGAA AGCTCACAAC CATTGTCTTC TTTCTCGATG AAACTTTTGA AGAAATTTCA 
TATGACATGG CTACCACAGT GTCTGATGCT GTTGAGGAGC TAGCTGGAAC AATTAAACTA 
TCAGCTTTCT CTAGCTTTAG TTTGTTTGAA TGTCGTAAAG TTGTTTCAAG TTCTAAATCA 
TCTGATCCCG GAAATGAGGA ATATATAGGA TTGGATGATA ACAAGTATAT TGGAGATCTC 
CTCGCAGAAT TTAAAGCTAT TAAAGATCGA AATAAAGGAG AGATACTACA CTGCAAACTG 
GTATTTAAAA AAAAATTATT CCGAGAGTCT GATGAAGCTG TAACAGATCT GATGTTTGTG 
CAACTTTCGT ATGTTCAACT GCAACATGAC TATTTGCTAG GAAACTATCC TGTTGGGAGG 
GACGATGCTG CACAGCTTTG TGCCTTGCAA ATTCTTGTTG GGATTGGGTT TGTCAATAGT 
CCAGAGTCAT GCATTGATTG GACATCACTT CTTGAGCGGT TTTTGCCAAG ACAAATAGCA 
ATAACCCGAG CAAAGCGTGA ATGGGAATTG GATATCCTTG CTCGCTACCG TTCAATGGAG 
AACGTGACCA AAGATGATGC AAGACAACAA TTTCTACGGA TACTGAAGGC ACTGCCATAC 
GGGAATTCAG TTTTTTTTAG CGTACGCAAG ATAGATGATC CGATCGGTCT TTTACCTGGG 
CGAATCATTT TGGGTATCAA CAAACGTGGG GTTCACTTTT TTCGACCGGT TCCTAAAGAA 
TATCTGCACT CTGCTGAACT ACGTGACATC ATGCAATTTG GCAGCAGTAA CACTGCTGTC 
TTTTTCAAAA TGAGAGTCGC TGGTGTTCTT CACATATTTC AGTTCGAGAC AAAACAGGGA 
GAAGAAATTT GTGTTGCTTT GCAAACACAT ATAAATGATG TCATGTTGCG TCGTTACTCC 
AAAGCTCGAT CTGCTGCCAA TAGCTTGGTT AATGGAGATA TATCTTGTAG TTCTAAGCCG 
CAAAATTTTG AAGTGTATGA AAAACGTTTG CAAGATTTGT CTAAGGCTTA TGAAGAGTCC 
CAAAAGAAGA TTGAGAAGTT GATGGATGAA CAACAAGAGA AAAATCAGCA AGAAGTTACT 
CTGCGTGAAG AGTTAGAAGC TATACACAAT GGTTTGGAGC TTGAAAGGAG AAAATTGTTG 
GAGGTTACTT TAGACCGAGA TAAACTTAGG TCATTGTGTG ACGAGAAGGG AACAACTATT 
CAATCCTTGA TGTCTGAACT GCGAGGAATG GAAGCAAGGT TGGCAAAGTC GGGCAACACA 
AAATCAAGTA AAGAGACAAA ATCAGAATTA GCCGAAATGA ATAATCAGAT ATTATACAAG 
ATCCAAAAGG AGTTAGAAGT TCGAAATAAG GAATTGCATG TCGCAGTTGA TAATTCAAAG 
AGGTTGTTGA GTGAAAACAA GATATTGGAG CAAAATCTCA ATATTGAAAA GAAGAAAAAA 
GAGGAGGTTG AAATTCATCA AAAGAGATAT GAACAAGAAA AAAAGGTGTT AAAGCTTCGA 
GTTTCTGAAC TTGAAAATAA GCTTGAAGTA CTTGCTCAAG ACTTGGATAG TGCTGAGTCT 
ACGATTGAAA GTAAGAATTC TGACATGCTG CTGTTGCAAA ATAACTTGAA AGAACTTGAG 
GAGTTAAGAG AAATGAAAGA GGACATTGAC AGAAAAAATG AGCAAACAGC TGCCATTTTG 
AAAATGCAAG GAGCCCAACT TGCTGAGCTA GAAATACTTT ATAAGGAAGA ACAAGTTTTA 
AGGAAAAGAT ACTATAATAC CATAGAAGAT ATGAAAGGGA AGATTAGAGT TTATTGTCGA 
ATAAGACCTC TAAATGAAAA AGAGAGTTCA GAGAGGGAAA AACAAATGCT GACAACTGTG 
GATGAGTTTA CTGTTGAACA TCCATGGAAA GACGACAAAA GAAAGCAACA CATATATGAT 
CGCGTATTTG ACATGCGTGC TAGTCAAGAT GATATCTTTG AAGACACAAA GTATTTGGTA 
CAGTCGGCTG TAGATGGGTA TAACGTTTGC ATCTTTGCAT ATGGTCAAAC TGGTTCTGGA 
AAAACTTTCA CTATATATGG GCATGAGAGC AATCCTGGAC TCACACCTCG AGCTACAAAG 
GAACTGTTCA ACATATTAAA GCGAGATAGC AAGAGATTTT CATTTTCTCT AAAGGCATAT 
ATGGTGGAAC TTTATCAAGA CACACTTGTA GACCTTTTGT TACCAAAAAG TGCAAGACGC 
TTGAAACTAG AGATTAAAAA AGATTCAAAG GGAATGGTCT TTGTAGAGAA TGTGACAACT 
ATTCCTATAT CAACTTTGGA GGAACTGCGA ATGATTCTTG AACGGGGATC GGAACGACGA 
CATGTTTCTG GAACAAATAT GAATGAAGAA AGCTCAAGAT CTCACCTCAT ATTATCAGTT 
GTTATTGAAA GTATTGATCT TCAAACCCAG TCTGCTGCGA GGGGCAAGCT GAGTTTTGTG 
GATCTTGCTG GTTCTGAGAG AGTTAAAAAG TCGGGCTCAG CTGGTTGTCA ACTCAAAGAA 
GCTCAAAGTA TCAACAAATC ACTTTCTGCA TTAGGTGATG TTATTGGTGC TTTATCTTCT 
GGCAACCAGC ATATTCCTTA TAGGAATCAC AAGCTAACGA TGTTGATGAG CGATTCATTG 
GGCGGCAATG CCAAGACGTT AATGTTTGTT AATGTGTCTC CAGCCGAATC AAATTTGGAC 
GAGACGTACA ATTCTCTTCT ATATGCATCG AGAGTGAGAA CGATCGTGAA TGATCCCAGC 
AAACATATAT CATCCAAAGA GATGGTGCGA TTGAAGAAGT TGGTAGCATA CTGGAAAGAG 
CAAGCCGGTA AAAAAGGTGA GGAAGAAGAC TTGGTGGATA TTGAGGAAGA TCGTACACGA 
AAAGATGAGG CAGATAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190628.1
CDS	54..3854
Translation	MEGQRGSNSSLSSGNGTEVATDVSSCFYVPNPSGTDFDAESSSLPPLLSLCHSSPAPQVALSIPAELAAAIPLIDRFQVEAFLRLMQKQIQSAGKRGFFYSKKSSGSNVRERFTFEDMLCFQKDPIPTSLLKINSDLVSRATKLFHLILKYMGVDSSDRSTPPSLDERIDLVGKLFKKTLKRVELRDELFAQISKQTRHNPDRQYLIKAWELMYLCASSMPPSKDIGGYLSEYIHNVAHDATIEPDAQVLAVNTLKALKRSIKAGPRHTTPGREEIEALLTGRKLTTIVFFLDETFEEISYDMATTVSDAVEELAGTIKLSAFSSFSLFECRKVVSSSKSSDPGNEEYIGLDDNKYIGDLLAEFKAIKDRNKGEILHCKLVFKKKLFRESDEAVTDLMFVQLSYVQLQHDYLLGNYPVGRDDAAQLCALQILVGIGFVNSPESCIDWTSLLERFLPRQIAITRAKREWELDILARYRSMENVTKDDARQQFLRILKALPYGNSVFFSVRKIDDPIGLLPGRIILGINKRGVHFFRPVPKEYLHSAELRDIMQFGSSNTAVFFKMRVAGVLHIFQFETKQGEEICVALQTHINDVMLRRYSKARSAANSLVNGDISCSSKPQNFEVYEKRLQDLSKAYEESQKKIEKLMDEQQEKNQQEVTLREELEAIHNGLELERRKLLEVTLDRDKLRSLCDEKGTTIQSLMSELRGMEARLAKSGNTKSSKETKSELAEMNNQILYKIQKELEVRNKELHVAVDNSKRLLSENKILEQNLNIEKKKKEEVEIHQKRYEQEKKVLKLRVSELENKLEVLAQDLDSAESTIESKNSDMLLLQNNLKELEELREMKEDIDRKNEQTAAILKMQGAQLAELEILYKEEQVLRKRYYNTIEDMKGKIRVYCRIRPLNEKESSEREKQMLTTVDEFTVEHPWKDDKRKQHIYDRVFDMRASQDDIFEDTKYLVQSAVDGYNVCIFAYGQTGSGKTFTIYGHESNPGLTPRATKELFNILKRDSKRFSFSLKAYMVELYQDTLVDLLLPKSARRLKLEIKKDSKGMVFVENVTTIPISTLEELRMILERGSERRHVSGTNMNEESSRSHLILSVVIESIDLQTQSAARGKLSFVDLAGSERVKKSGSAGCQLKEAQSINKSLSALGDVIGALSSGNQHIPYRNHKLTMLMSDSLGGNAKTLMFVNVSPAESNLDETYNSLLYASRVRTIVNDPSKHISSKEMVRLKKLVAYWKEQAGKKGEEEDLVDIEEDRTRKDEADS

Target ORF information:

RefSeq Version	NM_001203699.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana kinesin-like calmodulin-binding protein (ZWICHEL) (ZWI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203699.1

ORF Insert Sequence:

ATGGAGGGCC AACGAGGCAG TAATTCTTCG TTGAGCTCTG GCAATGGCAC CGAAGTCGCC 
ACCGACGTTT CTTCTTGCTT CTATGTTCCC AATCCCTCTG GAACCGATTT CGATGCCGAG 
TCGTCTTCTC TTCCTCCTCT TCTCTCGCTC TGTCACAGCT CCCCAGCTCC TCAAGTGGCA 
TTGTCAATTC CTGCGGAGCT TGCCGCCGCC ATTCCCCTCA TCGATCGCTT CCAGGTTGAA 
GCTTTTCTGC GGCTAATGCA GAAACAAATC CAGTCTGCTG GGAAGCGTGG CTTCTTCTAT 
TCCAAAAAGT CCTCTGGCTC CAATGTCCGC GAGCGCTTCA CATTTGAGGA TATGCTTTGC 
TTTCAAAAGG ATCCAATCCC CACATCTCTC CTCAAGATTA ACAGCGATCT CGTCAGCCGT 
GCTACCAAGT TGTTTCATCT CATCTTAAAA TATATGGGTG TTGATTCATC TGATCGATCT 
ACGCCTCCCA GTTTAGATGA ACGCATTGAC CTCGTTGGAA AGCTCTTCAA AAAAACTTTG 
AAGCGTGTTG AACTCAGGGA CGAACTTTTT GCCCAAATCT CCAAACAGAC TAGACATAAT 
CCTGACAGGC AATACTTGAT CAAAGCTTGG GAATTGATGT ACTTATGTGC CTCCTCTATG 
CCTCCTAGCA AAGATATCGG TGGATATCTA TCTGAGTATA TTCATAATGT CGCACACGAT 
GCAACTATTG AACCGGATGC TCAGGTTCTT GCTGTTAACA CTTTGAAAGC TTTAAAGCGC 
TCTATCAAAG CTGGTCCTAG GCACACCACA CCTGGTCGTG AAGAAATTGA AGCCCTTTTG 
ACCGGTAGAA AGCTCACAAC CATTGTCTTC TTTCTCGATG AAACTTTTGA AGAAATTTCA 
TATGACATGG CTACCACAGT GTCTGATGCT GTTGAGGAGC TAGCTGGAAC AATTAAACTA 
TCAGCTTTCT CTAGCTTTAG TTTGTTTGAA TGTCGTAAAG TTGTTTCAAG TTCTAAATCA 
TCTGATCCCG GAAATGAGGA ATATATAGGA TTGGATGATA ACAAGTATAT TGGAGATCTC 
CTCGCAGAAT TTAAAGCTAT TAAAGATCGA AATAAAGGAG AGATACTACA CTGCAAACTG 
GTATTTAAAA AAAAATTATT CCGAGAGTCT GATGAAGCTG TAACAGATCT GATGTTTGTG 
CAACTTTCGT ATGTTCAACT GCAACATGAC TATTTGCTAG GAAACTATCC TGTTGGGAGG 
GACGATGCTG CACAGCTTTG TGCCTTGCAA ATTCTTGTTG GGATTGGGTT TGTCAATAGT 
CCAGAGTCAT GCATTGATTG GACATCACTT CTTGAGCGGT TTTTGCCAAG ACAAATAGCA 
ATAACCCGAG CAAAGCGTGA ATGGGAATTG GATATCCTTG CTCGCTACCG TTCAATGGAG 
AACGTGACCA AAGATGATGC AAGACAACAA TTTCTACGGA TACTGAAGGC ACTGCCATAC 
GGGAATTCAG TTTTTTTTAG CGTACGCAAG ATAGATGATC CGATCGGTCT TTTACCTGGG 
CGAATCATTT TGGGTATCAA CAAACGTGGG GTTCACTTTT TTCGACCGGT TCCTAAAGAA 
TATCTGCACT CTGCTGAACT ACGTGACATC ATGCAATTTG GCAGCAGTAA CACTGCTGTC 
TTTTTCAAAA TGAGAGTCGC TGGTGTTCTT CACATATTTC AGTTCGAGAC AAAACAGGGA 
GAAGAAATTT GTGTTGCTTT GCAAACACAT ATAAATGATG TCATGTTGCG TCGTTACTCC 
AAAGCTCGAT CTGCTGCCAA TAGCTTGGTT AATGGAGATA TATCTTGTAG TTCTAAGCCG 
CAAAATTTTG AAGTGTATGA AAAACGTTTG CAAGATTTGT CTAAGGCTTA TGAAGAGTCC 
CAAAAGAAGA TTGAGAAGTT GATGGATGAA CAACAAGAGA AAAATCAGCA AGAAGTTACT 
CTGCGTGAAG AGTTAGAAGC TATACACAAT GGTTTGGAGC TTGAAAGGAG AAAATTGTTG 
GAGGTTACTT TAGACCGAGA TAAACTTAGG TCATTGTGTG ACGAGAAGGG AACAACTATT 
CAATCCTTGA TGTCTGAACT GCGAGGAATG GAAGCAAGGT TGGCAAAGTC GGGCAACACA 
AAATCAAGTA AAGAGACAAA ATCAGAATTA GCCGAAATGA ATAATCAGAT ATTATACAAG 
ATCCAAAAGG AGTTAGAAGT TCGAAATAAG GAATTGCATG TCGCAGTTGA TAATTCAAAG 
AGGTTGTTGA GTGAAAACAA GATATTGGAG CAAAATCTCA ATATTGAAAA GAAGAAAAAA 
GAGGAGGTTG AAATTCATCA AAAGAGATAT GAACAAGAAA AAAAGGTGTT AAAGCTTCGA 
GTTTCTGAAC TTGAAAATAA GCTTGAAGTA CTTGCTCAAG ACTTGGATAG TGCTGAGTCT 
ACGATTGAAA GTAAGAATTC TGACATGCTG CTGTTGCAAA ATAACTTGAA AGAACTTGAG 
GAGTTAAGAG AAATGAAAGA GGACATTGAC AGAAAAAATG AGCAAACAGC TGCCATTTTG 
AAAATGCAAG GAGCCCAACT TGCTGAGCTA GAAATACTTT ATAAGGAAGA ACAAGTTTTA 
AGGAAAAGAT ACTATAATAC CATAGAAGAT ATGAAAGGGA AGATTAGAGT TTATTGTCGA 
ATAAGACCTC TAAATGAAAA AGAGAGTTCA GAGAGGGAAA AACAAATGCT GACAACTGTG 
GATGAGTTTA CTGTTGAACA TCCATGGAAA GACGACAAAA GAAAGCAACA CATATATGAT 
CGCGTATTTG ACATGCGTGC TAGTCAAGAT GATATCTTTG AAGACACAAA GTATTTGGTA 
CAGTCGGCTG TAGATGGGTA TAACGTTTGC ATCTTTGCAT ATGGTCAAAC TGGTTCTGGA 
AAAACTTTCA CTATATATGG GCATGAGAGC AATCCTGGAC TCACACCTCG AGCTACAAAG 
GAACTGTTCA ACATATTAAA GCGAGATAGC AAGAGATTTT CATTTTCTCT AAAGGCATAT 
ATGGTGGAAC TTTATCAAGA CACACTTGTA GACCTTTTGT TACCAAAAAG TGCAAGACGC 
TTGAAACTAG AGATTAAAAA AGATTCAAAG GGAATGGTCT TTGTAGAGAA TGTGACAACT 
ATTCCTATAT CAACTTTGGA GGAACTGCGA ATGATTCTTG AACGGGGATC GGAACGACGA 
CATGTTTCTG GAACAAATAT GAATGAAGAA AGCTCAAGAT CTCACCTCAT ATTATCAGTT 
GTTATTGAAA GTATTGATCT TCAAACCCAG TCTGCTGCGA GGGGCAAGCT GAGTTTTGTG 
GATCTTGCTG GTTCTGAGAG AGTTAAAAAG TCGGGCTCAG CTGGTTGTCA ACTCAAAGAA 
GCTCAAAGTA TCAACAAATC ACTTTCTGCA TTAGGTGATG TTATTGGTGC TTTATCTTCT 
GGCAACCAGC ATATTCCTTA TAGGAATCAC AAGCTAACGA TGTTGATGAG CGATTCATTG 
GGCGGCAATG CCAAGACGTT AATGTTTGTT AATGTGTCTC CAGCCGAATC AAATTTGGAC 
GAGACGTACA ATTCTCTTCT ATATGCATCG AGAGTGAGAA CGATCGTGAA TGATCCCAGC 
AAACATATAT CATCCAAAGA GATGGTGCGA TTGAAGAAGT TGGTAGCATA CTGGAAAGAG 
CAAGCCGGTA AAAAAGGTGA GGAAGAAGAC TTGGTGGATA TTGAGGAAGA TCGTACACGA 
AAAGATGAGG CAGATAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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ZWI cDNA ORF clone, Arabidopsis thaliana(thale cress)

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