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ORF cDNA Clones and Custom Clones » Species Summary » Bos taurus » LOC100139548 cDNA ORF clone
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LOC100139548 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol LOC100139548
Entrez Gene ID 100139548
Full Name helicase SKI2W
Synonyms SKIV2L
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_003584612.4 XP_003584660.2 helicase SKI2W
XM_003587695.4 XP_003587743.1 helicase SKI2W
XM_003587695.5 XP_003587743.1 helicase SKI2W

Pathways from BioSystems


Homologies


Anopheles gambiae (African malaria mosquito) AgaP_AGAP003182 XP_312880.4
Homo sapiens (human) SKIV2L NP_008860.4
Danio rerio (zebrafish) skiv2l NP_001038366.1
Schizosaccharomyces pombe (fission yeast) SPCC550.03c NP_588095.1
Canis lupus familiaris (dog) SKIV2L XP_538841.2
Bos taurus (cattle) SKIV2L XP_003587743.1
Arabidopsis thaliana (thale cress) AT3G46960 NP_190280.5
Pan troglodytes (chimpanzee) SKIV2L XP_001160927.2
SKIV2L XP_001106475.2
Rattus norvegicus (Norway rat) Skiv2l NP_998724.1
Drosophila melanogaster (fruit fly) tst NP_524465.1
Caenorhabditis elegans (roundworm) skih-2 NP_502084.2
Saccharomyces cerevisiae (baker's yeast) SKI2 NP_013502.3
Xenopus tropicalis (tropical clawed frog) skiv2l XP_004917184.1
Mus musculus (house mouse) Skiv2l NP_067312.2
Eremothecium gossypii AGOS_AER067C NP_984927.2
Neurospora crassa NCU01857 XP_965488.1

Gene Ontology

The following LOC100139548 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100139548 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa11261 XM_003587695.5
Latest version!		 Bos taurus helicase SKI2W (LOC100139548), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa11261 XM_003584612.4
Latest version!		 Bos taurus helicase SKI2W (LOC100139548), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa11261 XM_003587695.4 Bos taurus helicase SKI2W (LOC100139548), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa11261
Clone ID Related Accession (Same CDS sequence) XM_003584612.4 , XM_003587695.4 , XM_003587695.5
Accession Version XM_003584612.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product helicase SKI2W
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646599.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003584612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG 
AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG 
TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA 
TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG 
CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC 
ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT 
GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT 
CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG 
GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC 
ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA 
GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG 
GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT 
CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT 
GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG 
CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG 
GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT 
ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA 
CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC 
CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT 
GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG 
GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG 
GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG 
CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC 
GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT 
CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG 
GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG 
CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT 
GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC 
ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG 
GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG 
GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG 
GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG 
GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG 
ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA 
GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT 
CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC 
GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC 
CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC 
TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC 
GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC 
ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG 
GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG 
AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC 
GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA 
TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG 
CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC 
CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT 
GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003584660.2
CDS96..3836
Translation

Target ORF information:

RefSeq Version XM_003584612.4
Organism Bos taurus(cattle)
Definition Bos taurus helicase SKI2W (LOC100139548), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003584612.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG 
AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG 
TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA 
TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG 
CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC 
ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT 
GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT 
CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG 
GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC 
ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA 
GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG 
GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT 
CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT 
GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG 
CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG 
GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT 
ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA 
CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC 
CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT 
GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG 
GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG 
GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG 
CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC 
GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT 
CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG 
GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG 
CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT 
GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC 
ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG 
GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG 
GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG 
GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG 
GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG 
ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA 
GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT 
CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC 
GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC 
CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC 
TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC 
GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC 
ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG 
GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG 
AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC 
GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA 
TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG 
CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC 
CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT 
GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa11261
Clone ID Related Accession (Same CDS sequence) XM_003584612.4 , XM_003587695.4 , XM_003587695.5
Accession Version XM_003587695.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product helicase SKI2W
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587695.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG 
AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG 
TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA 
TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG 
CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC 
ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT 
GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT 
CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG 
GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC 
ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA 
GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG 
GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT 
CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT 
GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG 
CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG 
GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT 
ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA 
CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC 
CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT 
GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG 
GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG 
GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG 
CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC 
GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT 
CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG 
GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG 
CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT 
GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC 
ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG 
GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG 
GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG 
GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG 
GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG 
ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA 
GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT 
CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC 
GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC 
CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC 
TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC 
GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC 
ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG 
GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG 
AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC 
GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA 
TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG 
CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC 
CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT 
GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003587743.1
CDS96..3836
Translation

Target ORF information:

RefSeq Version XM_003587695.4
Organism Bos taurus(cattle)
Definition Bos taurus helicase SKI2W (LOC100139548), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003587695.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG 
AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG 
TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA 
TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG 
CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC 
ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT 
GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT 
CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG 
GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC 
ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA 
GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG 
GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT 
CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT 
GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG 
CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG 
GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT 
ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA 
CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC 
CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT 
GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG 
GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG 
GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG 
CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC 
GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT 
CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG 
GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG 
CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT 
GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC 
ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG 
GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG 
GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG 
GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG 
GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG 
ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA 
GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT 
CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC 
GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC 
CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC 
TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC 
GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC 
ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG 
GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG 
AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC 
GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA 
TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG 
CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC 
CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT 
GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa11261
Clone ID Related Accession (Same CDS sequence) XM_003584612.4 , XM_003587695.4 , XM_003587695.5
Accession Version XM_003587695.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product helicase SKI2W
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587695.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG 
AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG 
TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA 
TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG 
CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC 
ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT 
GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT 
CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG 
GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC 
ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA 
GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG 
GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT 
CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT 
GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG 
CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG 
GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT 
ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA 
CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC 
CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT 
GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG 
GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG 
GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG 
CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC 
GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT 
CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG 
GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG 
CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT 
GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC 
ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG 
GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG 
GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG 
GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG 
GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG 
ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA 
GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT 
CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC 
GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC 
CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC 
TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC 
GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC 
ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG 
GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG 
AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC 
GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA 
TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG 
CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC 
CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT 
GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003587743.1
CDS98..3838
Translation

Target ORF information:

RefSeq Version XM_003587695.5
Organism Bos taurus(cattle)
Definition Bos taurus helicase SKI2W (LOC100139548), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003587695.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG 
AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG 
TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA 
TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG 
CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC 
ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT 
GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT 
CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG 
GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC 
ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA 
GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG 
GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT 
CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT 
GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG 
CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG 
GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT 
ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA 
CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC 
CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT 
GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG 
GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG 
GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG 
CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC 
GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT 
CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG 
GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG 
CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT 
GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC 
ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG 
GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG 
GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG 
GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG 
GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG 
ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA 
GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT 
CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC 
GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC 
CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC 
TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC 
GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC 
ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG 
GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG 
AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC 
GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA 
TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG 
CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC 
CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT 
GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed