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HCN2 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol HCN2
Entrez Gene ID 100297361
Full Name hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
General protein information
Preferred Names

hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2

Names

potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
hyperpolarization activated cyclic nucleotide gated potassium channel 2
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_002704862.5 XP_002704908.3 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X2
XM_024995092.1 XP_024850860.1 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
XM_002689161.4 XP_002689207.2 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) HCN2 NP_001185.3
Gallus gallus (chicken) HCN2 XP_003642915.1
Xenopus tropicalis (tropical clawed frog) hcn2 XP_002939815.2
Canis lupus familiaris (dog) HCN2 XP_005642548.1
Rattus norvegicus (Norway rat) Hcn2 NP_446136.1
Pan troglodytes (chimpanzee) LOC467640 XP_003952669.1
Bos taurus (cattle) HCN2 XP_002689207.2
Mus musculus (house mouse) Hcn2 NP_032252.1
Danio rerio (zebrafish) hcn2b XP_685414.3

Gene Ontology

The following HCN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236626 XM_024995092.1
Latest version!		 Bos taurus hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa21459 XM_002689161.4
Latest version!		 Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 2 (HCN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa90324 XM_002704862.5
Latest version!		 Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 2 (HCN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa236626
Clone ID Related Accession (Same CDS sequence) XM_024995092.1
Accession Version XM_024995092.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2712bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACGCGC GCGGGGGCGG CGGGCGGCCC GGGGAGAGCC CGGGCGCGAC CCCCGCGCCG 
GGGCCGCCGC CGCCGCCGCC GCCGCCCGCG GCCCCCCAGC AGCAGCCGCC GCCGCCGCCA 
CCACCCGCGC CCCCTCCGGG GCTCGGACCG GCGCCCCCTC AGCAGGCGCC CCGGACCGAG 
GCGGCGCCCC CGGAGGCAGC AGATGAGAGC GGCCCGCGGG CCCGGCTCCG CAGCCGAGAC 
AGCTCTTGCG GCCGCCCGGG CACCCCGGGC GCCGCGAGCA CGGCCAAGGG CAGCCCGAAC 
GGCGAGTGCG GGCGCGGCGA GCCGCAGTGC AGCCCCGCGG GGTCCGAGGG CCCGGCGCGG 
GGTCCCAAGG TGTCGTTCTC GTGCCGCGGG GCAGCCCCGG GGCCCGGGCC GGGCTCGGGC 
GCCGGGCCCG GGCCGGCGGA CGAGGCGGGC AGCGAGGAGG CGGGCCCGGC GGGGGAGTCG 
CGCGGCAGCC AGGCCAGCTT CATGCAGCGC CAGTTCGGCG CGCTCCTGCA GCCGGGCGTC 
AACAAGTTCT CGTTGCGGAT GTTCGGCAGT CAGAAGGCCG TGGAGCGCGA GCAGGAGCGC 
GTTAAGTCAG CGGGGGCCTG GATCATCCAC CCTTACAGCG ACTTCAGGTT CTACTGGGAC 
TTCACCATGC TGCTCTTCAT GGTGGGAAAC CTCATCATCA TCCCCGTGGG CATCACCTTC 
TTCAAGGACG AGACCACGGC CCCATGGATT GTGTTCAATG TTGTCTCGGA CACATTCTTC 
CTCATGGACC TGGTGCTGAA CTTCCGCACG GGCATTGTGA TCGAGGACAA CACGGAGATC 
ATCCTGGACC CCGAGAAGAT CAAGAAGAAG TACCTGCGCA CGTGGTTCGT GGTGGACTTC 
GTATCCTCCA TCCCCGTGGA CTACATCTTC CTCATCGTGG AGAAAGGCAT CGACTCTGAG 
GTCTACAAGA CGGCCCGCGC CCTGCGCATC GTGCGCTTCA CCAAGATCCT CAGCCTGCTG 
CGCCTGCTCC GCTTGTCGCG CCTCATCCGC TACATCCATC AGTGGGAGGA GATCTTCCAC 
ATGACCTACG ACCTGGCGAG CGCCGTCATG CGCATCTGCA ACCTCATCAG CATGATGCTG 
CTCCTCTGCC ACTGGGATGG CTGCCTGCAG TTCCTGGTGC CCATGCTTCA GGACTTCCCA 
CGCAACTGCT GGGTCTCCAT CAACGGCATG GTGAACCACT CATGGAGCGA GCTCTACTCC 
TTCGCGCTGT TCAAGGCCAT GAGCCACATG CTGTGCATCG GGTACGGGCG GCAGGCGCCA 
GAAAGCATGA CGGACATCTG GCTGACCATG CTGAGCATGA TCGTGGGTGC CACCTGCTAC 
GCCATGTTCA TTGGCCACGC CACCGCCCTC ATCCAGTCGC TGGACTCCTC AAGGCGCCAG 
TACCAGGAGA AGTACAAGCA AGTGGAGCAG TACATGTCCT TCCACAAGCT GCCAGCCGAC 
TTCCGCCAGA AGATCCACGA CTACTACGAG CACCGCTACC AGGGCAAGAT GTTCGACGAG 
GACAGCATCC TCGGCGAGCT CAACGGGCCC CTGCGGGAGG AGATCGTCAA CTTCAACTGC 
CGGAAGCTGG TGGCCTCCAT GCCACTGTTC GCCAATGCTG ACCCCAACTT CGTCACGGCC 
ATGCTGACCA AGCTCAAGTT TGAGGTCTTC CAGCCAGGCG ACTACATCAT CCGTGAGGGC 
ACCATTGGCA AGAAGATGTA CTTCATCCAA CACGGCGTGG TCAGTGTGCT TACCAAGGGC 
AACAAGGAGA TGAAGTTGTC TGATGGCTCC TACTTTGGGG AGATCTGCCT GCTGACGCGG 
GGCCGGCGCA CGGCGAGCGT GCGGGCCGAC ACCTACTGCC GCCTCTACTC GCTGAGTGTG 
GACAACTTCA ATGAGGTGCT GGAGGAGTAC CCCATGATGA GGCGGGCCTT TGAGACAGTC 
GCCATTGACC GCCTGGATCG CATTGGCAAG AAGAACTCGA TCCTGCTACA CAAGGTGCAG 
CACGACCTCA ACTCTGGCGT GTTTAACAAC CAGGAGAACG CCATCATCCA GGAGATTGTC 
AAGTATGACC GCGAGATGGT GCAGCAGGCT GAGCTGGGCC AGCGTGTCGG CCTCTTCCCG 
CCACCACCGC CACCTCCACA GGGCACCTCA GCCATTGCCA CGCTGCAGCA GGCCGTGGCC 
ATGAGCTTCT GTCCACAAGT CGCACGCCCG CTTGTTGGGC CCCTGGCACT CGGCTCGCCG 
CGCCTCGTGC GCCGCCTGCC CCCGGGGCCT GCGCCCACTG TCGCCACTGC TGCCGCCTCA 
CCCGGACCCG CCCCTGCAGC CAGTCCCCCT GGCGCGCCTG CCAGCCCCAG GGCACCGCGG 
ACCTCGCCCT TCGGTGGCGC ACCCGGCTCC CCTGCTGCGC CCCGCGCTGG GCCTGCGCTA 
CCTGCGCGGC GCCTAAGCCG CGCCTCGCGC CCGCTGTCTG CCTCGCAGCC CTCGCTGCCC 
CACGGCGCAC CCGGCCCTGC GGCCACGGCG CGACCGGCCA GCAGCTCCAC GCCGCGCCTG 
GGGCCCACGC CCGCCACCCG GGTCGCGGCG CCCAGCCCGG ACCGTAGGGA CTCAGCCTCG 
CCCTCACCGG GCACCGCCGG CGGACCCGAC CCGCTGGACT CCGCGCGCTC ACGCCTTTCG 
TCCAACTTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024850860.1
CDS1..2712
Translation

Target ORF information:

RefSeq Version XM_024995092.1
Organism Bos taurus(cattle)
Definition Bos taurus hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024995092.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACGCGC GCGGGGGCGG CGGGCGGCCC GGGGAGAGCC CGGGCGCGAC CCCCGCGCCG 
GGGCCGCCGC CGCCGCCGCC GCCGCCCGCG GCCCCCCAGC AGCAGCCGCC GCCGCCGCCA 
CCACCCGCGC CCCCTCCGGG GCTCGGACCG GCGCCCCCTC AGCAGGCGCC CCGGACCGAG 
GCGGCGCCCC CGGAGGCAGC AGATGAGAGC GGCCCGCGGG CCCGGCTCCG CAGCCGAGAC 
AGCTCTTGCG GCCGCCCGGG CACCCCGGGC GCCGCGAGCA CGGCCAAGGG CAGCCCGAAC 
GGCGAGTGCG GGCGCGGCGA GCCGCAGTGC AGCCCCGCGG GGTCCGAGGG CCCGGCGCGG 
GGTCCCAAGG TGTCGTTCTC GTGCCGCGGG GCAGCCCCGG GGCCCGGGCC GGGCTCGGGC 
GCCGGGCCCG GGCCGGCGGA CGAGGCGGGC AGCGAGGAGG CGGGCCCGGC GGGGGAGTCG 
CGCGGCAGCC AGGCCAGCTT CATGCAGCGC CAGTTCGGCG CGCTCCTGCA GCCGGGCGTC 
AACAAGTTCT CGTTGCGGAT GTTCGGCAGT CAGAAGGCCG TGGAGCGCGA GCAGGAGCGC 
GTTAAGTCAG CGGGGGCCTG GATCATCCAC CCTTACAGCG ACTTCAGGTT CTACTGGGAC 
TTCACCATGC TGCTCTTCAT GGTGGGAAAC CTCATCATCA TCCCCGTGGG CATCACCTTC 
TTCAAGGACG AGACCACGGC CCCATGGATT GTGTTCAATG TTGTCTCGGA CACATTCTTC 
CTCATGGACC TGGTGCTGAA CTTCCGCACG GGCATTGTGA TCGAGGACAA CACGGAGATC 
ATCCTGGACC CCGAGAAGAT CAAGAAGAAG TACCTGCGCA CGTGGTTCGT GGTGGACTTC 
GTATCCTCCA TCCCCGTGGA CTACATCTTC CTCATCGTGG AGAAAGGCAT CGACTCTGAG 
GTCTACAAGA CGGCCCGCGC CCTGCGCATC GTGCGCTTCA CCAAGATCCT CAGCCTGCTG 
CGCCTGCTCC GCTTGTCGCG CCTCATCCGC TACATCCATC AGTGGGAGGA GATCTTCCAC 
ATGACCTACG ACCTGGCGAG CGCCGTCATG CGCATCTGCA ACCTCATCAG CATGATGCTG 
CTCCTCTGCC ACTGGGATGG CTGCCTGCAG TTCCTGGTGC CCATGCTTCA GGACTTCCCA 
CGCAACTGCT GGGTCTCCAT CAACGGCATG GTGAACCACT CATGGAGCGA GCTCTACTCC 
TTCGCGCTGT TCAAGGCCAT GAGCCACATG CTGTGCATCG GGTACGGGCG GCAGGCGCCA 
GAAAGCATGA CGGACATCTG GCTGACCATG CTGAGCATGA TCGTGGGTGC CACCTGCTAC 
GCCATGTTCA TTGGCCACGC CACCGCCCTC ATCCAGTCGC TGGACTCCTC AAGGCGCCAG 
TACCAGGAGA AGTACAAGCA AGTGGAGCAG TACATGTCCT TCCACAAGCT GCCAGCCGAC 
TTCCGCCAGA AGATCCACGA CTACTACGAG CACCGCTACC AGGGCAAGAT GTTCGACGAG 
GACAGCATCC TCGGCGAGCT CAACGGGCCC CTGCGGGAGG AGATCGTCAA CTTCAACTGC 
CGGAAGCTGG TGGCCTCCAT GCCACTGTTC GCCAATGCTG ACCCCAACTT CGTCACGGCC 
ATGCTGACCA AGCTCAAGTT TGAGGTCTTC CAGCCAGGCG ACTACATCAT CCGTGAGGGC 
ACCATTGGCA AGAAGATGTA CTTCATCCAA CACGGCGTGG TCAGTGTGCT TACCAAGGGC 
AACAAGGAGA TGAAGTTGTC TGATGGCTCC TACTTTGGGG AGATCTGCCT GCTGACGCGG 
GGCCGGCGCA CGGCGAGCGT GCGGGCCGAC ACCTACTGCC GCCTCTACTC GCTGAGTGTG 
GACAACTTCA ATGAGGTGCT GGAGGAGTAC CCCATGATGA GGCGGGCCTT TGAGACAGTC 
GCCATTGACC GCCTGGATCG CATTGGCAAG AAGAACTCGA TCCTGCTACA CAAGGTGCAG 
CACGACCTCA ACTCTGGCGT GTTTAACAAC CAGGAGAACG CCATCATCCA GGAGATTGTC 
AAGTATGACC GCGAGATGGT GCAGCAGGCT GAGCTGGGCC AGCGTGTCGG CCTCTTCCCG 
CCACCACCGC CACCTCCACA GGGCACCTCA GCCATTGCCA CGCTGCAGCA GGCCGTGGCC 
ATGAGCTTCT GTCCACAAGT CGCACGCCCG CTTGTTGGGC CCCTGGCACT CGGCTCGCCG 
CGCCTCGTGC GCCGCCTGCC CCCGGGGCCT GCGCCCACTG TCGCCACTGC TGCCGCCTCA 
CCCGGACCCG CCCCTGCAGC CAGTCCCCCT GGCGCGCCTG CCAGCCCCAG GGCACCGCGG 
ACCTCGCCCT TCGGTGGCGC ACCCGGCTCC CCTGCTGCGC CCCGCGCTGG GCCTGCGCTA 
CCTGCGCGGC GCCTAAGCCG CGCCTCGCGC CCGCTGTCTG CCTCGCAGCC CTCGCTGCCC 
CACGGCGCAC CCGGCCCTGC GGCCACGGCG CGACCGGCCA GCAGCTCCAC GCCGCGCCTG 
GGGCCCACGC CCGCCACCCG GGTCGCGGCG CCCAGCCCGG ACCGTAGGGA CTCAGCCTCG 
CCCTCACCGG GCACCGCCGG CGGACCCGAC CCGCTGGACT CCGCGCGCTC ACGCCTTTCG 
TCCAACTTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa21459
Clone ID Related Accession (Same CDS sequence) XM_002689161.4
Accession Version XM_002689161.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002689161.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGAGGGCA GCCCGAACGG CGAGTGCGGG CGCGGCGAGC CGCAGTGCAG CCCCGCGGGG 
TCCGAGGGCC CGGCGCGGGG TCCCAAGGTG TCGTTCTCGT GCCGCGGGGC AGCCCCGGGG 
CCCGGGCCGG GCTCGGGCGC CGGGCCCGGG CCGGCGGACG AGGCGGGCAG CGAGGAGGCG 
GGCCCGGCGG GGGAGTCGCG CGGCAGCCAG GCCAGCTTCA TGCAGCGCCA GTTCGGCGCG 
CTCCTGCAGC CGGGCGTCAA CAAGTTCTCG TTGCGGATGT TCGGCAGTCA GAAGGCCGTG 
GAGCGCGAGC AGGAGCGCGT TAAGTCAGCG GGGGCCTGGA TCATCCACCC TTACAGCGAC 
TTCAGGTTCT ACTGGGACTT CACCATGCTG CTCTTCATGG TGGGAAACCT CATCATCATC 
CCCGTGGGCA TCACCTTCTT CAAGGACGAG ACCACGGCCC CATGGATTGT GTTCAATGTT 
GTCTCGGACA CATTCTTCCT CATGGACCTG GTGCTGAACT TCCGCACGGG CATTGTGATC 
GAGGACAACA CGGAGATCAT CCTGGACCCC GAGAAGATCA AGAAGAAGTA CCTGCGCACG 
TGGTTCGTGG TGGACTTCGT ATCCTCCATC CCCGTGGACT ACATCTTCCT CATCGTGGAG 
AAAGGCATCG ACTCTGAGGT CTACAAGACG GCCCGCGCCC TGCGCATCGT GCGCTTCACC 
AAGATCCTCA GCCTGCTGCG CCTGCTCCGC TTGTCGCGCC TCATCCGCTA CATCCATCAG 
TGGGAGGAGA TCTTCCACAT GACCTACGAC CTGGCGAGCG CCGTCATGCG CATCTGCAAC 
CTCATCAGCA TGATGCTGCT CCTCTGCCAC TGGGATGGCT GCCTGCAGTT CCTGGTGCCC 
ATGCTTCAGG ACTTCCCACG CAACTGCTGG GTCTCCATCA ACGGCATGGT GAACCACTCA 
TGGAGCGAGC TCTACTCCTT CGCGCTGTTC AAGGCCATGA GCCACATGCT GTGCATCGGG 
TACGGGCGGC AGGCGCCAGA AAGCATGACG GACATCTGGC TGACCATGCT GAGCATGATC 
GTGGGTGCCA CCTGCTACGC CATGTTCATT GGCCACGCCA CCGCCCTCAT CCAGTCGCTG 
GACTCCTCAA GGCGCCAGTA CCAGGAGAAG TACAAGCAAG TGGAGCAGTA CATGTCCTTC 
CACAAGCTGC CAGCCGACTT CCGCCAGAAG ATCCACGACT ACTACGAGCA CCGCTACCAG 
GGCAAGATGT TCGACGAGGA CAGCATCCTC GGCGAGCTCA ACGGGCCCCT GCGGGAGGAG 
ATCGTCAACT TCAACTGCCG GAAGCTGGTG GCCTCCATGC CACTGTTCGC CAATGCTGAC 
CCCAACTTCG TCACGGCCAT GCTGACCAAG CTCAAGTTTG AGGTCTTCCA GCCAGGCGAC 
TACATCATCC GTGAGGGCAC CATTGGCAAG AAGATGTACT TCATCCAACA CGGCGTGGTC 
AGTGTGCTTA CCAAGGGCAA CAAGGAGATG AAGTTGTCTG ATGGCTCCTA CTTTGGGGAG 
ATCTGCCTGC TGACGCGGGG CCGGCGCACG GCGAGCGTGC GGGCCGACAC CTACTGCCGC 
CTCTACTCGC TGAGTGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTACCC CATGATGAGG 
CGGGCCTTTG AGACAGTCGC CATTGACCGC CTGGATCGCA TTGGCAAGAA GAACTCGATC 
CTGCTACACA AGGTGCAGCA CGACCTCAAC TCTGGCGTGT TTAACAACCA GGAGAACGCC 
ATCATCCAGG AGATTGTCAA GTATGACCGC GAGATGGTGC AGCAGGCTGA GCTGGGCCAG 
CGTGTCGGCC TCTTCCCGCC ACCACCGCCA CCTCCACAGG GCACCTCAGC CATTGCCACG 
CTGCAGCAGG CCGTGGCCAT GAGCTTCTGT CCACAAGTCG CACGCCCGCT TGTTGGGCCC 
CTGGCACTCG GCTCGCCGCG CCTCGTGCGC CGCCTGCCCC CGGGGCCTGC GCCCACTGTC 
GCCACTGCTG CCGCCTCACC CGGACCCGCC CCTGCAGCCA GTCCCCCTGG CGCGCCTGCC 
AGCCCCAGGG CACCGCGGAC CTCGCCCTTC GGTGGCGCAC CCGGCTCCCC TGCTGCGCCC 
CGCGCTGGGC CTGCGCTACC TGCGCGGCGC CTAAGCCGCG CCTCGCGCCC GCTGTCTGCC 
TCGCAGCCCT CGCTGCCCCA CGGCGCACCC GGCCCTGCGG CCACGGCGCG ACCGGCCAGC 
AGCTCCACGC CGCGCCTGGG GCCCACGCCC GCCACCCGGG TCGCGGCGCC CAGCCCGGAC 
CGTAGGGACT CAGCCTCGCC CTCACCGGGC ACCGCCGGCG GACCCGACCC GCTGGACTCC 
GCGCGCTCAC GCCTTTCGTC CAACTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002689207.2
CDS1..2430
Translation

Target ORF information:

RefSeq Version XM_002689161.4
Organism Bos taurus(cattle)
Definition Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 2 (HCN2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002689161.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGAGGGCA GCCCGAACGG CGAGTGCGGG CGCGGCGAGC CGCAGTGCAG CCCCGCGGGG 
TCCGAGGGCC CGGCGCGGGG TCCCAAGGTG TCGTTCTCGT GCCGCGGGGC AGCCCCGGGG 
CCCGGGCCGG GCTCGGGCGC CGGGCCCGGG CCGGCGGACG AGGCGGGCAG CGAGGAGGCG 
GGCCCGGCGG GGGAGTCGCG CGGCAGCCAG GCCAGCTTCA TGCAGCGCCA GTTCGGCGCG 
CTCCTGCAGC CGGGCGTCAA CAAGTTCTCG TTGCGGATGT TCGGCAGTCA GAAGGCCGTG 
GAGCGCGAGC AGGAGCGCGT TAAGTCAGCG GGGGCCTGGA TCATCCACCC TTACAGCGAC 
TTCAGGTTCT ACTGGGACTT CACCATGCTG CTCTTCATGG TGGGAAACCT CATCATCATC 
CCCGTGGGCA TCACCTTCTT CAAGGACGAG ACCACGGCCC CATGGATTGT GTTCAATGTT 
GTCTCGGACA CATTCTTCCT CATGGACCTG GTGCTGAACT TCCGCACGGG CATTGTGATC 
GAGGACAACA CGGAGATCAT CCTGGACCCC GAGAAGATCA AGAAGAAGTA CCTGCGCACG 
TGGTTCGTGG TGGACTTCGT ATCCTCCATC CCCGTGGACT ACATCTTCCT CATCGTGGAG 
AAAGGCATCG ACTCTGAGGT CTACAAGACG GCCCGCGCCC TGCGCATCGT GCGCTTCACC 
AAGATCCTCA GCCTGCTGCG CCTGCTCCGC TTGTCGCGCC TCATCCGCTA CATCCATCAG 
TGGGAGGAGA TCTTCCACAT GACCTACGAC CTGGCGAGCG CCGTCATGCG CATCTGCAAC 
CTCATCAGCA TGATGCTGCT CCTCTGCCAC TGGGATGGCT GCCTGCAGTT CCTGGTGCCC 
ATGCTTCAGG ACTTCCCACG CAACTGCTGG GTCTCCATCA ACGGCATGGT GAACCACTCA 
TGGAGCGAGC TCTACTCCTT CGCGCTGTTC AAGGCCATGA GCCACATGCT GTGCATCGGG 
TACGGGCGGC AGGCGCCAGA AAGCATGACG GACATCTGGC TGACCATGCT GAGCATGATC 
GTGGGTGCCA CCTGCTACGC CATGTTCATT GGCCACGCCA CCGCCCTCAT CCAGTCGCTG 
GACTCCTCAA GGCGCCAGTA CCAGGAGAAG TACAAGCAAG TGGAGCAGTA CATGTCCTTC 
CACAAGCTGC CAGCCGACTT CCGCCAGAAG ATCCACGACT ACTACGAGCA CCGCTACCAG 
GGCAAGATGT TCGACGAGGA CAGCATCCTC GGCGAGCTCA ACGGGCCCCT GCGGGAGGAG 
ATCGTCAACT TCAACTGCCG GAAGCTGGTG GCCTCCATGC CACTGTTCGC CAATGCTGAC 
CCCAACTTCG TCACGGCCAT GCTGACCAAG CTCAAGTTTG AGGTCTTCCA GCCAGGCGAC 
TACATCATCC GTGAGGGCAC CATTGGCAAG AAGATGTACT TCATCCAACA CGGCGTGGTC 
AGTGTGCTTA CCAAGGGCAA CAAGGAGATG AAGTTGTCTG ATGGCTCCTA CTTTGGGGAG 
ATCTGCCTGC TGACGCGGGG CCGGCGCACG GCGAGCGTGC GGGCCGACAC CTACTGCCGC 
CTCTACTCGC TGAGTGTGGA CAACTTCAAT GAGGTGCTGG AGGAGTACCC CATGATGAGG 
CGGGCCTTTG AGACAGTCGC CATTGACCGC CTGGATCGCA TTGGCAAGAA GAACTCGATC 
CTGCTACACA AGGTGCAGCA CGACCTCAAC TCTGGCGTGT TTAACAACCA GGAGAACGCC 
ATCATCCAGG AGATTGTCAA GTATGACCGC GAGATGGTGC AGCAGGCTGA GCTGGGCCAG 
CGTGTCGGCC TCTTCCCGCC ACCACCGCCA CCTCCACAGG GCACCTCAGC CATTGCCACG 
CTGCAGCAGG CCGTGGCCAT GAGCTTCTGT CCACAAGTCG CACGCCCGCT TGTTGGGCCC 
CTGGCACTCG GCTCGCCGCG CCTCGTGCGC CGCCTGCCCC CGGGGCCTGC GCCCACTGTC 
GCCACTGCTG CCGCCTCACC CGGACCCGCC CCTGCAGCCA GTCCCCCTGG CGCGCCTGCC 
AGCCCCAGGG CACCGCGGAC CTCGCCCTTC GGTGGCGCAC CCGGCTCCCC TGCTGCGCCC 
CGCGCTGGGC CTGCGCTACC TGCGCGGCGC CTAAGCCGCG CCTCGCGCCC GCTGTCTGCC 
TCGCAGCCCT CGCTGCCCCA CGGCGCACCC GGCCCTGCGG CCACGGCGCG ACCGGCCAGC 
AGCTCCACGC CGCGCCTGGG GCCCACGCCC GCCACCCGGG TCGCGGCGCC CAGCCCGGAC 
CGTAGGGACT CAGCCTCGCC CTCACCGGGC ACCGCCGGCG GACCCGACCC GCTGGACTCC 
GCGCGCTCAC GCCTTTCGTC CAACTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa90324
Clone ID Related Accession (Same CDS sequence) XM_002704862.5
Accession Version XM_002704862.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2904bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002704862.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 16% of CDS bases ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGGCACAG AGGAAGTGAT GACAAGGCTG GACCTTCTGG GGGTGCTGCC GAGGCTGGGG 
CTCAATATTG GTATGGAGGG GGCATGGGCA GCACCTTGGG GGACAGAGTG GTGGTCCATG 
TGGTGTGGGG AGGGGACCAG CTCCAAGATA GGCCACTGCT CGCAGGGCCG GGCCGTGGGG 
CACAGACACA CGGCTCCGCG TGTCTCCTTC ACCAAGTGTT CCTGGCTGTT CCTGGAGCAC 
GTGTTTGAGG GTCCCCAGAC TGTAGGCTCC ATGACTTCAA ACCTGGCTCT GGCCCGACTG 
AGCAGGAAGT GTGGGTGGTG TGCTGAGGAG GTGCTGAGAA TCTGTGGCAG GGGGCTGGAC 
AGCCAGATTT CAGGACAAAG GCTCCAGGCC CCGCCTGCTG GAGGCCCCCG GGCCCAGAAC 
GCGATGCCAG TGGATGGAGG CTCCCAATAT GATATCAAGT CTGAAAGCAC TCAGAAGGCC 
CGATGCCCGA ACGGCGAGTG CGGGCGCGGC GAGCCGCAGT GCAGCCCCGC GGGGTCCGAG 
GGCCCGGCGC GGGGTCCCAA GGTGTCGTTC TCGTGCCGCG GGGCAGCCCC GGGGCCCGGG 
CCGGGCTCGG GCGCCGGGCC CGGGCCGGCG GACGAGGCGG GCAGCGAGGA GGCGGGCCCG 
GCGGGGGAGT CGCGCGGCAG CCAGGCCAGC TTCATGCAGC GCCAGTTCGG CGCGCTCCTG 
CAGCCGGGCG TCAACAAGTT CTCGTTGCGG ATGTTCGGCA GTCAGAAGGC CGTGGAGCGC 
GAGCAGGAGC GCGTTAAGTC AGCGGGGGCC TGGATCATCC ACCCTTACAG CGACTTCAGG 
TTCTACTGGG ACTTCACCAT GCTGCTCTTC ATGGTGGGAA ACCTCATCAT CATCCCCGTG 
GGCATCACCT TCTTCAAGGA CGAGACCACG GCCCCATGGA TTGTGTTCAA TGTTGTCTCG 
GACACATTCT TCCTCATGGA CCTGGTGCTG AACTTCCGCA CGGGCATTGT GATCGAGGAC 
AACACGGAGA TCATCCTGGA CCCCGAGAAG ATCAAGAAGA AGTACCTGCG CACGTGGTTC 
GTGGTGGACT TCGTATCCTC CATCCCCGTG GACTACATCT TCCTCATCGT GGAGAAAGGC 
ATCGACTCTG AGGTCTACAA GACGGCCCGC GCCCTGCGCA TCGTGCGCTT CACCAAGATC 
CTCAGCCTGC TGCGCCTGCT CCGCTTGTCG CGCCTCATCC GCTACATCCA TCAGTGGGAG 
GAGATCTTCC ACATGACCTA CGACCTGGCG AGCGCCGTCA TGCGCATCTG CAACCTCATC 
AGCATGATGC TGCTCCTCTG CCACTGGGAT GGCTGCCTGC AGTTCCTGGT GCCCATGCTT 
CAGGACTTCC CACGCAACTG CTGGGTCTCC ATCAACGGCA TGGTGAACCA CTCATGGAGC 
GAGCTCTACT CCTTCGCGCT GTTCAAGGCC ATGAGCCACA TGCTGTGCAT CGGGTACGGG 
CGGCAGGCGC CAGAAAGCAT GACGGACATC TGGCTGACCA TGCTGAGCAT GATCGTGGGT 
GCCACCTGCT ACGCCATGTT CATTGGCCAC GCCACCGCCC TCATCCAGTC GCTGGACTCC 
TCAAGGCGCC AGTACCAGGA GAAGTACAAG CAAGTGGAGC AGTACATGTC CTTCCACAAG 
CTGCCAGCCG ACTTCCGCCA GAAGATCCAC GACTACTACG AGCACCGCTA CCAGGGCAAG 
ATGTTCGACG AGGACAGCAT CCTCGGCGAG CTCAACGGGC CCCTGCGGGA GGAGATCGTC 
AACTTCAACT GCCGGAAGCT GGTGGCCTCC ATGCCACTGT TCGCCAATGC TGACCCCAAC 
TTCGTCACGG CCATGCTGAC CAAGCTCAAG TTTGAGGTCT TCCAGCCAGG CGACTACATC 
ATCCGTGAGG GCACCATTGG CAAGAAGATG TACTTCATCC AACACGGCGT GGTCAGTGTG 
CTTACCAAGG GCAACAAGGA GATGAAGTTG TCTGATGGCT CCTACTTTGG GGAGATCTGC 
CTGCTGACGC GGGGCCGGCG CACGGCGAGC GTGCGGGCCG ACACCTACTG CCGCCTCTAC 
TCGCTGAGTG TGGACAACTT CAATGAGGTG CTGGAGGAGT ACCCCATGAT GAGGCGGGCC 
TTTGAGACAG TCGCCATTGA CCGCCTGGAT CGCATTGGCA AGAAGAACTC GATCCTGCTA 
CACAAGGTGC AGCACGACCT CAACTCTGGC GTGTTTAACA ACCAGGAGAA CGCCATCATC 
CAGGAGATTG TCAAGTATGA CCGCGAGATG GTGCAGCAGG CTGAGCTGGG CCAGCGTGTC 
GGCCTCTTCC CGCCACCACC GCCACCTCCA CAGGGCACCT CAGCCATTGC CACGCTGCAG 
CAGGCCGTGG CCATGAGCTT CTGTCCACAA GTCGCACGCC CGCTTGTTGG GCCCCTGGCA 
CTCGGCTCGC CGCGCCTCGT GCGCCGCCTG CCCCCGGGGC CTGCGCCCAC TGTCGCCACT 
GCTGCCGCCT CACCCGGACC CGCCCCTGCA GCCAGTCCCC CTGGCGCGCC TGCCAGCCCC 
AGGGCACCGC GGACCTCGCC CTTCGGTGGC GCACCCGGCT CCCCTGCTGC GCCCCGCGCT 
GGGCCTGCGC TACCTGCGCG GCGCCTAAGC CGCGCCTCGC GCCCGCTGTC TGCCTCGCAG 
CCCTCGCTGC CCCACGGCGC ACCCGGCCCT GCGGCCACGG CGCGACCGGC CAGCAGCTCC 
ACGCCGCGCC TGGGGCCCAC GCCCGCCACC CGGGTCGCGG CGCCCAGCCC GGACCGTAGG 
GACTCAGCCT CGCCCTCACC GGGCACCGCC GGCGGACCCG ACCCGCTGGA CTCCGCGCGC 
TCACGCCTTT CGTCCAACTT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002704908.3
CDS1..2904
Translation

Target ORF information:

RefSeq Version XM_002704862.5
Organism Bos taurus(cattle)
Definition Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 2 (HCN2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002704862.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGGCACAG AGGAAGTGAT GACAAGGCTG GACCTTCTGG GGGTGCTGCC GAGGCTGGGG 
CTCAATATTG GTATGGAGGG GGCATGGGCA GCACCTTGGG GGACAGAGTG GTGGTCCATG 
TGGTGTGGGG AGGGGACCAG CTCCAAGATA GGCCACTGCT CGCAGGGCCG GGCCGTGGGG 
CACAGACACA CGGCTCCGCG TGTCTCCTTC ACCAAGTGTT CCTGGCTGTT CCTGGAGCAC 
GTGTTTGAGG GTCCCCAGAC TGTAGGCTCC ATGACTTCAA ACCTGGCTCT GGCCCGACTG 
AGCAGGAAGT GTGGGTGGTG TGCTGAGGAG GTGCTGAGAA TCTGTGGCAG GGGGCTGGAC 
AGCCAGATTT CAGGACAAAG GCTCCAGGCC CCGCCTGCTG GAGGCCCCCG GGCCCAGAAC 
GCGATGCCAG TGGATGGAGG CTCCCAATAT GATATCAAGT CTGAAAGCAC TCAGAAGGCC 
CGATGCCCGA ACGGCGAGTG CGGGCGCGGC GAGCCGCAGT GCAGCCCCGC GGGGTCCGAG 
GGCCCGGCGC GGGGTCCCAA GGTGTCGTTC TCGTGCCGCG GGGCAGCCCC GGGGCCCGGG 
CCGGGCTCGG GCGCCGGGCC CGGGCCGGCG GACGAGGCGG GCAGCGAGGA GGCGGGCCCG 
GCGGGGGAGT CGCGCGGCAG CCAGGCCAGC TTCATGCAGC GCCAGTTCGG CGCGCTCCTG 
CAGCCGGGCG TCAACAAGTT CTCGTTGCGG ATGTTCGGCA GTCAGAAGGC CGTGGAGCGC 
GAGCAGGAGC GCGTTAAGTC AGCGGGGGCC TGGATCATCC ACCCTTACAG CGACTTCAGG 
TTCTACTGGG ACTTCACCAT GCTGCTCTTC ATGGTGGGAA ACCTCATCAT CATCCCCGTG 
GGCATCACCT TCTTCAAGGA CGAGACCACG GCCCCATGGA TTGTGTTCAA TGTTGTCTCG 
GACACATTCT TCCTCATGGA CCTGGTGCTG AACTTCCGCA CGGGCATTGT GATCGAGGAC 
AACACGGAGA TCATCCTGGA CCCCGAGAAG ATCAAGAAGA AGTACCTGCG CACGTGGTTC 
GTGGTGGACT TCGTATCCTC CATCCCCGTG GACTACATCT TCCTCATCGT GGAGAAAGGC 
ATCGACTCTG AGGTCTACAA GACGGCCCGC GCCCTGCGCA TCGTGCGCTT CACCAAGATC 
CTCAGCCTGC TGCGCCTGCT CCGCTTGTCG CGCCTCATCC GCTACATCCA TCAGTGGGAG 
GAGATCTTCC ACATGACCTA CGACCTGGCG AGCGCCGTCA TGCGCATCTG CAACCTCATC 
AGCATGATGC TGCTCCTCTG CCACTGGGAT GGCTGCCTGC AGTTCCTGGT GCCCATGCTT 
CAGGACTTCC CACGCAACTG CTGGGTCTCC ATCAACGGCA TGGTGAACCA CTCATGGAGC 
GAGCTCTACT CCTTCGCGCT GTTCAAGGCC ATGAGCCACA TGCTGTGCAT CGGGTACGGG 
CGGCAGGCGC CAGAAAGCAT GACGGACATC TGGCTGACCA TGCTGAGCAT GATCGTGGGT 
GCCACCTGCT ACGCCATGTT CATTGGCCAC GCCACCGCCC TCATCCAGTC GCTGGACTCC 
TCAAGGCGCC AGTACCAGGA GAAGTACAAG CAAGTGGAGC AGTACATGTC CTTCCACAAG 
CTGCCAGCCG ACTTCCGCCA GAAGATCCAC GACTACTACG AGCACCGCTA CCAGGGCAAG 
ATGTTCGACG AGGACAGCAT CCTCGGCGAG CTCAACGGGC CCCTGCGGGA GGAGATCGTC 
AACTTCAACT GCCGGAAGCT GGTGGCCTCC ATGCCACTGT TCGCCAATGC TGACCCCAAC 
TTCGTCACGG CCATGCTGAC CAAGCTCAAG TTTGAGGTCT TCCAGCCAGG CGACTACATC 
ATCCGTGAGG GCACCATTGG CAAGAAGATG TACTTCATCC AACACGGCGT GGTCAGTGTG 
CTTACCAAGG GCAACAAGGA GATGAAGTTG TCTGATGGCT CCTACTTTGG GGAGATCTGC 
CTGCTGACGC GGGGCCGGCG CACGGCGAGC GTGCGGGCCG ACACCTACTG CCGCCTCTAC 
TCGCTGAGTG TGGACAACTT CAATGAGGTG CTGGAGGAGT ACCCCATGAT GAGGCGGGCC 
TTTGAGACAG TCGCCATTGA CCGCCTGGAT CGCATTGGCA AGAAGAACTC GATCCTGCTA 
CACAAGGTGC AGCACGACCT CAACTCTGGC GTGTTTAACA ACCAGGAGAA CGCCATCATC 
CAGGAGATTG TCAAGTATGA CCGCGAGATG GTGCAGCAGG CTGAGCTGGG CCAGCGTGTC 
GGCCTCTTCC CGCCACCACC GCCACCTCCA CAGGGCACCT CAGCCATTGC CACGCTGCAG 
CAGGCCGTGG CCATGAGCTT CTGTCCACAA GTCGCACGCC CGCTTGTTGG GCCCCTGGCA 
CTCGGCTCGC CGCGCCTCGT GCGCCGCCTG CCCCCGGGGC CTGCGCCCAC TGTCGCCACT 
GCTGCCGCCT CACCCGGACC CGCCCCTGCA GCCAGTCCCC CTGGCGCGCC TGCCAGCCCC 
AGGGCACCGC GGACCTCGCC CTTCGGTGGC GCACCCGGCT CCCCTGCTGC GCCCCGCGCT 
GGGCCTGCGC TACCTGCGCG GCGCCTAAGC CGCGCCTCGC GCCCGCTGTC TGCCTCGCAG 
CCCTCGCTGC CCCACGGCGC ACCCGGCCCT GCGGCCACGG CGCGACCGGC CAGCAGCTCC 
ACGCCGCGCC TGGGGCCCAC GCCCGCCACC CGGGTCGCGG CGCCCAGCCC GGACCGTAGG 
GACTCAGCCT CGCCCTCACC GGGCACCGCC GGCGGACCCG ACCCGCTGGA CTCCGCGCGC 
TCACGCCTTT CGTCCAACTT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed