RGN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	RGN
Entrez Gene ID	280910
Full Name	regucalcin
Synonyms	GNL
General protein information	Preferred Names regucalcin Names regucalcin RC SMP-30 gluconolactonase regucalcin (senescence marker protein-30) senescence marker protein 30
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005228064.3	XP_005228121.1	regucalcin isoform X1
XM_005228065.3	XP_005228122.1	regucalcin isoform X2
XM_024987730.1	XP_024843498.1	regucalcin isoform X2
XM_005228066.3	XP_005228123.1	regucalcin isoform X2
XM_005228064.4	XP_005228121.1	regucalcin isoform X1
NM_173957.2	NP_776382.1	regucalcin
XM_005228065.4	XP_005228122.1	regucalcin isoform X2
XM_005228066.4	XP_005228123.1	regucalcin isoform X2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	regucalcin	NP_727588.2
Kluyveromyces lactis	KLLA0F04301g	XP_455274.1
Eremothecium gossypii	AGOS_ABL098W	NP_982849.1
Bos taurus (cattle)	RGN	NP_776382.1
Homo sapiens (human)	RGN	NP_004674.1
	RGN	XP_001090835.1
Mus musculus (house mouse)	Rgn	NP_033086.1
Danio rerio (zebrafish)	rgn	NP_991309.1
Drosophila melanogaster (fruit fly)	smp-30	NP_731942.1
Saccharomyces cerevisiae (baker's yeast)	YBR053C	NP_009609.1
Magnaporthe oryzae (rice blast fungus)	MGG_05015	XP_003712519.1
Neurospora crassa	NCU00326	XP_957797.1
Xenopus tropicalis (tropical clawed frog)	rgn	NP_001006822.1
Pan troglodytes (chimpanzee)	RGN	XP_003317492.1
Canis lupus familiaris (dog)	RGN	XP_538011.3
Rattus norvegicus (Norway rat)	Rgn	NP_113734.1
Gallus gallus (chicken)	RGN	NP_990060.1

Gene Ontology
Bibliography

Related articles in PubMed

Regucalcin expression in bovine tissues and its regulation by sex steroid hormones in accessory sex glands.
Starvaggi Cucuzza L, Divari S, Mulasso C, Biolatti B, Cannizzo FT
PloS one9(11)e113950(2014)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.
Misawa H, Yamaguchi M
International journal of molecular medicine6(2)191-6(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

estrogen administration down-regulated RGN gene expression in the accessory sex glands of veal calves and beef cattle, while androgen treatment reduced RGN gene expression only in the testis.
Title: Regucalcin expression in bovine tissues and its regulation by sex steroid hormones in accessory sex glands.

The following RGN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RGN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42857	NM_173957.2 Latest version!	Bos taurus regucalcin (RGN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa35509	XM_005228064.4 Latest version!	Bos taurus regucalcin (RGN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa35510	XM_024987730.1 Latest version!	Bos taurus regucalcin (RGN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa35510	XM_005228066.4 Latest version!	Bos taurus regucalcin (RGN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa35510	XM_005228065.4 Latest version!	Bos taurus regucalcin (RGN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OBa35509	XM_005228064.3	Bos taurus regucalcin (RGN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa35510	XM_005228065.3	Bos taurus regucalcin (RGN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa35510	XM_005228066.3	Bos taurus regucalcin (RGN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RGN ( NM_173957.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776382.1 Bos taurus regucalcin (RGN), mRNA.

Price & Availability↑

CloneID	OBa42857
Clone ID Related Accession (Same CDS sequence)	NM_173957.2
Accession Version	NM_173957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	regucalcin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB035446.1. On Jun 3, 2003 this sequence version replaced NM_173957.1. ##Evidence-Data-START## Transcript exon combination :: AB035446.1, DV788589.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145417, SAMN03145418 [ECO:0000348] ##Evidence-Data-END##

ATGTCTTCCA TTAAGATTGA GTGTGTTTTG CGGGAGAACT GCCACTGTGG TGAGTCTCCA 
GTGTGGGAGG AGGCGTCCAA CTCTCTGCTC TTCGTAGATA TTCCTGCCAA AAAGGTTTGC 
CGGTGGGATT CTCTCAGCAA GCAAGTGCAG CGAGTGACCG TGGATGCCCC TGTCAGCTCT 
GTGGCCCTTC GCCAGTCGGG AGGCTATGTT GCCACAGTTG GAACAAAGTT CTGTGCTTTG 
AACTGGGAAG ATCAGTCAGC AGTTGTCTTG GCCACAGTAG ATAAAGAGAA GAAAAACAAT 
CGATTCAATG ACGGGAAGGT GGATCCCGCT GGGAGATACT TTGCTGGTAC CATGGCTGAG 
GAAACAGCTC CGGCAGTTTT GGAGCGCCGC CAAGGGTCCC TGTACTCCCT CTTTCCTGAC 
CACCATGTGG AAAAGTACTT TGACCAGGTG GACATCTCCA ATGGTTTGGA TTGGTCTATG 
GACCACAAAA TCTTCTATTA CATAGATAGC CTGTCCTACT CCGTGGATGC CTTTGACTAT 
GACCTGCAGA CGGGAAAGAT CTCCAACCGC AGAAGTGTTT ACAAGCTGGA GAAGGAAGAA 
CAAATCCCAG ATGGAATGTG TATTGATGTT GAGGGGAAGC TCTGGGTGGC CTGTTACAAT 
GGAGGAAGAG TGATCCGTTT GGATCCTGAG ACAGGGAAAA GACTCCAAAC TGTGAAGTTG 
CCTGTTGATA AAACAACCTC ATGCTGCTTC GGAGGGAAGG ATTACTCTGA AATGTACGTG 
ACCTGTGCCC GGGATGGGTT GGACTCCAAG GGTCTTCTGC AGCAACCTGA GGCTGGTGGA 
ATTTTCAAGA TAACTGGCCT AGGAGTCAAA GGAATTCCTC CCTATCCTTA CACAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776382.1
CDS	98..997
Translation	MSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	NM_173957.2
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173957.2

ORF Insert Sequence:

ATGTCTTCCA TTAAGATTGA GTGTGTTTTG CGGGAGAACT GCCACTGTGG TGAGTCTCCA 
GTGTGGGAGG AGGCGTCCAA CTCTCTGCTC TTCGTAGATA TTCCTGCCAA AAAGGTTTGC 
CGGTGGGATT CTCTCAGCAA GCAAGTGCAG CGAGTGACCG TGGATGCCCC TGTCAGCTCT 
GTGGCCCTTC GCCAGTCGGG AGGCTATGTT GCCACAGTTG GAACAAAGTT CTGTGCTTTG 
AACTGGGAAG ATCAGTCAGC AGTTGTCTTG GCCACAGTAG ATAAAGAGAA GAAAAACAAT 
CGATTCAATG ACGGGAAGGT GGATCCCGCT GGGAGATACT TTGCTGGTAC CATGGCTGAG 
GAAACAGCTC CGGCAGTTTT GGAGCGCCGC CAAGGGTCCC TGTACTCCCT CTTTCCTGAC 
CACCATGTGG AAAAGTACTT TGACCAGGTG GACATCTCCA ATGGTTTGGA TTGGTCTATG 
GACCACAAAA TCTTCTATTA CATAGATAGC CTGTCCTACT CCGTGGATGC CTTTGACTAT 
GACCTGCAGA CGGGAAAGAT CTCCAACCGC AGAAGTGTTT ACAAGCTGGA GAAGGAAGAA 
CAAATCCCAG ATGGAATGTG TATTGATGTT GAGGGGAAGC TCTGGGTGGC CTGTTACAAT 
GGAGGAAGAG TGATCCGTTT GGATCCTGAG ACAGGGAAAA GACTCCAAAC TGTGAAGTTG 
CCTGTTGATA AAACAACCTC ATGCTGCTTC GGAGGGAAGG ATTACTCTGA AATGTACGTG 
ACCTGTGCCC GGGATGGGTT GGACTCCAAG GGTCTTCTGC AGCAACCTGA GGCTGGTGGA 
ATTTTCAAGA TAACTGGCCT AGGAGTCAAA GGAATTCCTC CCTATCCTTA CACAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RGN ( XM_005228064.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228121.1 Bos taurus regucalcin (RGN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35509
Clone ID Related Accession (Same CDS sequence)	XM_005228064.3 , XM_005228064.4
Accession Version	XM_005228064.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regucalcin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAGT GTTGTTTTTG TTCTCTTTTT TACAGAAACT TTGAAAAAAA AACACTGGTC 
TCCCCCATGA CAATGTCTTC CATTAAGATT GAGTGTGTTT TGCGGGAGAA CTGCCACTGT 
GGTGAGTCTC CAGTGTGGGA GGAGGCGTCC AACTCTCTGC TCTTCGTAGA TATTCCTGCC 
AAAAAGGTTT GCCGGTGGGA TTCTCTCAGC AAGCAAGTGC AGCGAGTGAC CGTGGATGCC 
CCTGTCAGCT CTGTGGCCCT TCGCCAGTCG GGAGGCTATG TTGCCACAGT TGGAACAAAG 
TTCTGTGCTT TGAACTGGGA AGATCAGTCA GCAGTTGTCT TGGCCACAGT AGATAAAGAG 
AAGAAAAACA ATCGATTCAA TGACGGGAAG GTGGATCCCG CTGGGAGATA CTTTGCTGGT 
ACCATGGCTG AGGAAACAGC TCCGGCAGTT TTGGAGCGCC GCCAAGGGTC CCTGTACTCC 
CTCTTTCCTG ACCACCATGT GGAAAAGTAC TTTGACCAGG TGGACATCTC CAATGGTTTG 
GATTGGTCTA TGGACCACAA AATCTTCTAT TACATAGATA GCCTGTCCTA CTCCGTGGAT 
GCCTTTGACT ATGACCTGCA GACGGGAAAG ATCTCCAACC GCAGAAGTGT TTACAAGCTG 
GAGAAGGAAG AACAAATCCC AGATGGAATG TGTATTGATG TTGAGGGGAA GCTCTGGGTG 
GCCTGTTACA ATGGAGGAAG AGTGATCCGT TTGGATCCTG AGACAGGGAA AAGACTCCAA 
ACTGTGAAGT TGCCTGTTGA TAAAACAACC TCATGCTGCT TCGGAGGGAA GGATTACTCT 
GAAATGTACG TGACCTGTGC CCGGGATGGG TTGGACTCCA AGGGTCTTCT GCAGCAACCT 
GAGGCTGGTG GAATTTTCAA GATAACTGGC CTAGGAGTCA AAGGAATTCC TCCCTATCCT 
TACACAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228121.1
CDS	71..1042
Translation	MLECCFCSLFYRNFEKKTLVSPMTMSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	XM_005228064.3
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228064.3

ORF Insert Sequence:

ATGTTGGAGT GTTGTTTTTG TTCTCTTTTT TACAGAAACT TTGAAAAAAA AACACTGGTC 
TCCCCCATGA CAATGTCTTC CATTAAGATT GAGTGTGTTT TGCGGGAGAA CTGCCACTGT 
GGTGAGTCTC CAGTGTGGGA GGAGGCGTCC AACTCTCTGC TCTTCGTAGA TATTCCTGCC 
AAAAAGGTTT GCCGGTGGGA TTCTCTCAGC AAGCAAGTGC AGCGAGTGAC CGTGGATGCC 
CCTGTCAGCT CTGTGGCCCT TCGCCAGTCG GGAGGCTATG TTGCCACAGT TGGAACAAAG 
TTCTGTGCTT TGAACTGGGA AGATCAGTCA GCAGTTGTCT TGGCCACAGT AGATAAAGAG 
AAGAAAAACA ATCGATTCAA TGACGGGAAG GTGGATCCCG CTGGGAGATA CTTTGCTGGT 
ACCATGGCTG AGGAAACAGC TCCGGCAGTT TTGGAGCGCC GCCAAGGGTC CCTGTACTCC 
CTCTTTCCTG ACCACCATGT GGAAAAGTAC TTTGACCAGG TGGACATCTC CAATGGTTTG 
GATTGGTCTA TGGACCACAA AATCTTCTAT TACATAGATA GCCTGTCCTA CTCCGTGGAT 
GCCTTTGACT ATGACCTGCA GACGGGAAAG ATCTCCAACC GCAGAAGTGT TTACAAGCTG 
GAGAAGGAAG AACAAATCCC AGATGGAATG TGTATTGATG TTGAGGGGAA GCTCTGGGTG 
GCCTGTTACA ATGGAGGAAG AGTGATCCGT TTGGATCCTG AGACAGGGAA AAGACTCCAA 
ACTGTGAAGT TGCCTGTTGA TAAAACAACC TCATGCTGCT TCGGAGGGAA GGATTACTCT 
GAAATGTACG TGACCTGTGC CCGGGATGGG TTGGACTCCA AGGGTCTTCT GCAGCAACCT 
GAGGCTGGTG GAATTTTCAA GATAACTGGC CTAGGAGTCA AAGGAATTCC TCCCTATCCT 
TACACAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RGN ( XM_005228064.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228121.1 Bos taurus regucalcin (RGN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35509
Clone ID Related Accession (Same CDS sequence)	XM_005228064.3 , XM_005228064.4
Accession Version	XM_005228064.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	regucalcin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228064.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAGT GTTGTTTTTG TTCTCTTTTT TACAGAAACT TTGAAAAAAA AACACTGGTC 
TCCCCCATGA CAATGTCTTC CATTAAGATT GAGTGTGTTT TGCGGGAGAA CTGCCACTGT 
GGTGAGTCTC CAGTGTGGGA GGAGGCGTCC AACTCTCTGC TCTTCGTAGA TATTCCTGCC 
AAAAAGGTTT GCCGGTGGGA TTCTCTCAGC AAGCAAGTGC AGCGAGTGAC CGTGGATGCC 
CCTGTCAGCT CTGTGGCCCT TCGCCAGTCG GGAGGCTATG TTGCCACAGT TGGAACAAAG 
TTCTGTGCTT TGAACTGGGA AGATCAGTCA GCAGTTGTCT TGGCCACAGT AGATAAAGAG 
AAGAAAAACA ATCGATTCAA TGACGGGAAG GTGGATCCCG CTGGGAGATA CTTTGCTGGT 
ACCATGGCTG AGGAAACAGC TCCGGCAGTT TTGGAGCGCC GCCAAGGGTC CCTGTACTCC 
CTCTTTCCTG ACCACCATGT GGAAAAGTAC TTTGACCAGG TGGACATCTC CAATGGTTTG 
GATTGGTCTA TGGACCACAA AATCTTCTAT TACATAGATA GCCTGTCCTA CTCCGTGGAT 
GCCTTTGACT ATGACCTGCA GACGGGAAAG ATCTCCAACC GCAGAAGTGT TTACAAGCTG 
GAGAAGGAAG AACAAATCCC AGATGGAATG TGTATTGATG TTGAGGGGAA GCTCTGGGTG 
GCCTGTTACA ATGGAGGAAG AGTGATCCGT TTGGATCCTG AGACAGGGAA AAGACTCCAA 
ACTGTGAAGT TGCCTGTTGA TAAAACAACC TCATGCTGCT TCGGAGGGAA GGATTACTCT 
GAAATGTACG TGACCTGTGC CCGGGATGGG TTGGACTCCA AGGGTCTTCT GCAGCAACCT 
GAGGCTGGTG GAATTTTCAA GATAACTGGC CTAGGAGTCA AAGGAATTCC TCCCTATCCT 
TACACAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228121.1
CDS	249..1220
Translation	MLECCFCSLFYRNFEKKTLVSPMTMSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	XM_005228064.4
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228064.4

ORF Insert Sequence:

ATGTTGGAGT GTTGTTTTTG TTCTCTTTTT TACAGAAACT TTGAAAAAAA AACACTGGTC 
TCCCCCATGA CAATGTCTTC CATTAAGATT GAGTGTGTTT TGCGGGAGAA CTGCCACTGT 
GGTGAGTCTC CAGTGTGGGA GGAGGCGTCC AACTCTCTGC TCTTCGTAGA TATTCCTGCC 
AAAAAGGTTT GCCGGTGGGA TTCTCTCAGC AAGCAAGTGC AGCGAGTGAC CGTGGATGCC 
CCTGTCAGCT CTGTGGCCCT TCGCCAGTCG GGAGGCTATG TTGCCACAGT TGGAACAAAG 
TTCTGTGCTT TGAACTGGGA AGATCAGTCA GCAGTTGTCT TGGCCACAGT AGATAAAGAG 
AAGAAAAACA ATCGATTCAA TGACGGGAAG GTGGATCCCG CTGGGAGATA CTTTGCTGGT 
ACCATGGCTG AGGAAACAGC TCCGGCAGTT TTGGAGCGCC GCCAAGGGTC CCTGTACTCC 
CTCTTTCCTG ACCACCATGT GGAAAAGTAC TTTGACCAGG TGGACATCTC CAATGGTTTG 
GATTGGTCTA TGGACCACAA AATCTTCTAT TACATAGATA GCCTGTCCTA CTCCGTGGAT 
GCCTTTGACT ATGACCTGCA GACGGGAAAG ATCTCCAACC GCAGAAGTGT TTACAAGCTG 
GAGAAGGAAG AACAAATCCC AGATGGAATG TGTATTGATG TTGAGGGGAA GCTCTGGGTG 
GCCTGTTACA ATGGAGGAAG AGTGATCCGT TTGGATCCTG AGACAGGGAA AAGACTCCAA 
ACTGTGAAGT TGCCTGTTGA TAAAACAACC TCATGCTGCT TCGGAGGGAA GGATTACTCT 
GAAATGTACG TGACCTGTGC CCGGGATGGG TTGGACTCCA AGGGTCTTCT GCAGCAACCT 
GAGGCTGGTG GAATTTTCAA GATAACTGGC CTAGGAGTCA AAGGAATTCC TCCCTATCCT 
TACACAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RGN ( XM_005228065.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228122.1 Bos taurus regucalcin (RGN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35510
Clone ID Related Accession (Same CDS sequence)	XM_005228065.3 , XM_024987730.1 , XM_005228066.3 , XM_005228065.4 , XM_005228066.4
Accession Version	XM_005228065.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regucalcin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228065.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228122.1
CDS	113..1018
Translation	MTMSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	XM_005228065.3
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228065.3

ORF Insert Sequence:

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RGN ( XM_024987730.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843498.1 Bos taurus regucalcin (RGN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35510
Clone ID Related Accession (Same CDS sequence)	XM_005228065.3 , XM_024987730.1 , XM_005228066.3 , XM_005228065.4 , XM_005228066.4
Accession Version	XM_024987730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	regucalcin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843498.1
CDS	261..1166
Translation	MTMSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	XM_024987730.1
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987730.1

ORF Insert Sequence:

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RGN ( XM_005228066.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228123.1 Bos taurus regucalcin (RGN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35510
Clone ID Related Accession (Same CDS sequence)	XM_005228065.3 , XM_024987730.1 , XM_005228066.3 , XM_005228065.4 , XM_005228066.4
Accession Version	XM_005228066.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regucalcin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104730.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228123.1
CDS	107..1012
Translation	MTMSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	XM_005228066.3
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228066.3

ORF Insert Sequence:

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RGN ( XM_005228065.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228122.1 Bos taurus regucalcin (RGN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35510
Clone ID Related Accession (Same CDS sequence)	XM_005228065.3 , XM_024987730.1 , XM_005228066.3 , XM_005228065.4 , XM_005228066.4
Accession Version	XM_005228065.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	regucalcin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228065.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228122.1
CDS	147..1052
Translation	MTMSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	XM_005228065.4
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228065.4

ORF Insert Sequence:

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RGN ( XM_005228066.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228123.1 Bos taurus regucalcin (RGN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa35510
Clone ID Related Accession (Same CDS sequence)	XM_005228065.3 , XM_024987730.1 , XM_005228066.3 , XM_005228065.4 , XM_005228066.4
Accession Version	XM_005228066.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	regucalcin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228066.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228123.1
CDS	149..1054
Translation	MTMSSIKIECVLRENCHCGESPVWEEASNSLLFVDIPAKKVCRWDSLSKQVQRVTVDAPVSSVALRQSGGYVATVGTKFCALNWEDQSAVVLATVDKEKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERRQGSLYSLFPDHHVEKYFDQVDISNGLDWSMDHKIFYYIDSLSYSVDAFDYDLQTGKISNRRSVYKLEKEEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDSKGLLQQPEAGGIFKITGLGVKGIPPYPYTG

Target ORF information:

RefSeq Version	XM_005228066.4
Organism	Bos taurus(cattle)
Definition	Bos taurus regucalcin (RGN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228066.4

ORF Insert Sequence:

ATGACAATGT CTTCCATTAA GATTGAGTGT GTTTTGCGGG AGAACTGCCA CTGTGGTGAG 
TCTCCAGTGT GGGAGGAGGC GTCCAACTCT CTGCTCTTCG TAGATATTCC TGCCAAAAAG 
GTTTGCCGGT GGGATTCTCT CAGCAAGCAA GTGCAGCGAG TGACCGTGGA TGCCCCTGTC 
AGCTCTGTGG CCCTTCGCCA GTCGGGAGGC TATGTTGCCA CAGTTGGAAC AAAGTTCTGT 
GCTTTGAACT GGGAAGATCA GTCAGCAGTT GTCTTGGCCA CAGTAGATAA AGAGAAGAAA 
AACAATCGAT TCAATGACGG GAAGGTGGAT CCCGCTGGGA GATACTTTGC TGGTACCATG 
GCTGAGGAAA CAGCTCCGGC AGTTTTGGAG CGCCGCCAAG GGTCCCTGTA CTCCCTCTTT 
CCTGACCACC ATGTGGAAAA GTACTTTGAC CAGGTGGACA TCTCCAATGG TTTGGATTGG 
TCTATGGACC ACAAAATCTT CTATTACATA GATAGCCTGT CCTACTCCGT GGATGCCTTT 
GACTATGACC TGCAGACGGG AAAGATCTCC AACCGCAGAA GTGTTTACAA GCTGGAGAAG 
GAAGAACAAA TCCCAGATGG AATGTGTATT GATGTTGAGG GGAAGCTCTG GGTGGCCTGT 
TACAATGGAG GAAGAGTGAT CCGTTTGGAT CCTGAGACAG GGAAAAGACT CCAAACTGTG 
AAGTTGCCTG TTGATAAAAC AACCTCATGC TGCTTCGGAG GGAAGGATTA CTCTGAAATG 
TACGTGACCT GTGCCCGGGA TGGGTTGGAC TCCAAGGGTC TTCTGCAGCA ACCTGAGGCT 
GGTGGAATTT TCAAGATAAC TGGCCTAGGA GTCAAAGGAA TTCCTCCCTA TCCTTACACA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regucalcin expression in bovine tissues and its regulation by sex steroid hormones in accessory sex glands.
PloS one9(11)e113950(2014)
Starvaggi Cucuzza L,Divari S,Mulasso C,Biolatti B,Cannizzo FT

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.
International journal of molecular medicine6(2)191-6(2000 Aug)
Misawa H,Yamaguchi M

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RGN cDNA ORF clone, Bos taurus(cattle)

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