EEF2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	EEF2
Entrez Gene ID	281138
Full Name	eukaryotic translation elongation factor 2
Synonyms	EF-2
General protein information	Preferred Names eukaryotic translation elongation factor 2 Names elongation factor 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 7 Map Location: 7q15

mRNA and Protein(s)

mRNA Protein Name

NM_001075121.1 NP_001068589.1 elongation factor 2
Pathways from BioSystems

mRNA	Protein	Name
NM_001075121.1	NP_001068589.1	elongation factor 2

Homologies

Pan troglodytes (chimpanzee)	LOC100614367	XP_003316060.2
	EEF2	XP_001118006.2
Drosophila melanogaster (fruit fly)	Ef2b	NP_525105.2
Mus musculus (house mouse)	Eef2	NP_031933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009441	XP_307854.2
Danio rerio (zebrafish)	eef2b	NP_956752.2
Arabidopsis thaliana (thale cress)	LOS1	NP_849818.1
Oryza sativa (rice)	Os01g0742200	NP_001044211.1
Bos taurus (cattle)	EEF2	NP_001068589.1
Gallus gallus (chicken)	EEF2	NP_990699.1
Caenorhabditis elegans (roundworm)	eef-2	NP_001251010.1
Homo sapiens (human)	EEF2	NP_001952.1
Canis lupus familiaris (dog)	EEF2	XP_533949.3
Rattus norvegicus (Norway rat)	Eef2	NP_058941.1
Xenopus tropicalis (tropical clawed frog)	eef2.1	NP_989255.1

Gene Ontology
Bibliography

Related articles in PubMed

Eukaryotic elongation factor-2 (eEF2): its regulation and peptide chain elongation.
Kaul G, Pattan G, Rafeequi T
Cell biochemistry and function29(3)227-34(2011 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Localization of ZNF164, ZNF146, GGTA1, SOX2, PRLR and EEF2 on homoeologous cattle, sheep and goat chromosomes by fluorescent in situ hybridization and comparison with the human gene map.
Hayes H, Le Chalony C, Goubin G, Mercier D, Payen E, Bignon C, Kohno K
Cytogenetics and cell genetics72(4)342-6(1996)

Glucose induced genes in bovine aortic smooth muscle cells identified by mRNA differential display.
Nishio Y, Aiello LP, King GL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology8(1)103-6(1994 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eukaryotic elongation factor-2 (eEF2) may be a limiting factor in milk protein synthesis (Review)
Title: Eukaryotic elongation factor-2 (eEF2): its regulation and peptide chain elongation.

The following EEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43557	NM_001075121.1 Latest version!	Bos taurus eukaryotic translation elongation factor 2 (EEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF2 ( NM_001075121.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068589.1 Bos taurus eukaryotic translation elongation factor 2 (EEF2), mRNA.

Price & Availability↑

CloneID	OBa43557
Clone ID Related Accession (Same CDS sequence)	NM_001075121.1
Accession Version	NM_001075121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	elongation factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103385.1. ##Evidence-Data-START## Transcript exon combination :: BC103385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAACT TCACAGTAGA CCAGATCCGG GCCATCATGG ACAAGAAGGC CAACATCCGG 
AACATGTCTG TCATTGCCCA CGTGGACCAT GGCAAATCCA CTCTGACGGA CTCCCTTGTG 
TGCAAGGCGG GTATCATCGC CTCTGCCCGG GCTGGGGAGA CCCGCTTCAC TGACACCCGG 
AAGGATGAGC AGGAGCGTTG CATCACCATC AAGTCAACGG CCATCTCCCT TTTCTACGAG 
CTTTCAGAGA ACGACTTGAA TTTCATCAAG CAGAGCAAGG ATGGCTCTGG CTTCCTCATC 
AACCTCATCG ACTCCCCTGG GCACGTCGAC TTTTCCTCAG AGGTGACAGC TGCACTCCGT 
GTCACTGACG GTGCCTTGGT GGTGGTGGAC TGCGTGTCGG GTGTGTGTGT GCAGACAGAG 
ACGGTGCTGC GCCAGGCCAT CGCTGAGCGC ATCAAGCCAG TGCTGATGAT GAACAAGATG 
GACCGGGCCC TGCTTGAGCT GCAGCTGGAG CCTGAGGAGC TCTACCAAAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAACGT CATCATCTCC ACTTACGGCG AGGGCGAGAG CGGCCCCATG 
GGCAACATCA TGATCGACCC CGTCCTTGGT ACTGTTGGCT TCGGGTCTGG TCTCCACGGT 
TGGGCCTTTA CCCTGAAGCA GTTTGCCGAG ATGTATGTGG CCAAGTTTGC TGCCAAGGGC 
GAGGGACAGC TAGGGCCTGC TGAGCGGGCC AAGAAGGTGG AGGACATGAT GAAAAAGCTG 
TGGGGAGACC GGTACTTTGA TCCAGCCACC GGCAAGTTCA GCAAGTCAGC CAACAGCCCT 
GATGGCAAGA AGCTGCCACG GACGTTCTGC CAGCTCATCC TGGACCCAAT CTTCAAGGTG 
TTTGATGCAA TCATGAACTT CAAGAAAGAG GAGACTGCAA AACTAATTGA AAAACTGGAC 
ATCAAGTTGG ACAGTGAAGA TAAGGACAAG GAGGGCAAAC CGCTTCTGAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCTGG GGATGCCCTG CTGCAGATGA TCACCATCCA CCTGCCTTCC 
CCTGTGACGG CCCAGAAGTA CCGCTGTGAG CTCCTGTACG AGGGGCCCCC AGACGATGAG 
GCAGCCATGG GCATTAAAAG CTGTGATCCC AAAGGCCCTC TCATGATGTA CATTTCCAAA 
ATGGTGCCGA CCTCTGACAA AGGTCGGTTC TATGCTTTCG GCCGGGTCTT CTCAGGGCTG 
GTGTCTACTG GCCTGAAGGT CCGCATCATG GGACCCAACT ATACTCCTGG GAAGAAGGAG 
GACCTGTACC TGAAGCCGAT CCAGAGGACA ATCCTGATGA TGGGCCGTTA CGTGGAGCCC 
ATCGAGGATG TGCCTTGTGG CAACATTGTG GGCCTGGTGG GCGTGGACCA GTTCCTGGTG 
AAGACTGGCA CCATCACCAC CTTTGAGCAT GCCCACAACA TGCGGGTGAT GAAGTTCAGT 
GTGAGCCCTG TTGTCAGGGT TGCTGTGGAG GCCAAGAACC CGGCCGACCT GCCCAAGCTG 
GTGGAAGGTC TGAAGCGGCT GGCCAAGTCG GACCCCATGG TGCAGTGCAT CATCGAGGAG 
TCCGGGGAGC ACATTATCGC GGGGGCTGGG GAGCTGCACT TGGAGATCTG CCTCAAGGAC 
CTGGAGGAGG ATCACGCTTG CATCCCCATC AAGAAATCTG ACCCAGTTGT CTCGTACCGG 
GAGACTGTCA GCGAGGAGTC AAACGTGCTC TGCCTATCCA AGTCCCCCAA CAAGCACAAT 
AGGCTTTATA TGAAGGCGCG GCCCTTCCCT GACGGCCTGG CTGAGGACAT CGACAAGGGC 
GAGGTGTCCG CCCGCCAGGA GCTCAAGCAG CGGGCCCGCT ACCTGGCTGA GAAGTACGAG 
TGGGATGTGG CCGAGGCCCG TAAGATCTGG TGCTTCGGCC CTGATGGCAC GGGCCCCAAC 
ATCCTCACGG ACATCACCAA GGGTGTGCAA TACCTCAATG AAATCAAGGA CAGTGTGGTA 
GCTGGCTTCC AGTGGGCCAC CAAAGAGGGG GCACTGTGCG AGGAGAACAT GCGGGGCGTG 
CGCTTTGACG TCCATGATGT GACGCTGCAC GCTGATGCCA TCCACCGTGG GGGTGGCCAG 
ATCATCCCCA CGGCTCGGCG CTGCCTGTAT GCCAGTGTGC TGACTGCCCA GCCCCGGCTC 
ATGGAGCCCA TCTATCTTGT GGAGATCCAG TGTCCAGAAC AAGTGGTTGG TGGCATCTAC 
GGTGTCCTGA ACAGGAAGCG GGGCCACGTC TTTGAGGAGA CCCAGGTGGC CGGCACCCCC 
ATGTTTGTTG TGAAGGCCTA CCTTCCTGTC AATGAGTCCT TTGGCTTCAC TGCTGACCTG 
AGGTCCAACA CGGGCGGCCA GGCCTTCCCC CAGTGTGTGT TCGACCACTG GCAGATCCTG 
CCCGGTGACC CCTTTGACAA CACCAGCCGC CCCAGTCAGG TAGTGGCGGA GACACGCAAG 
CGCAAAGGCC TGAAGGAAGG CATCCCAGCC CTGGACAACT TCCTGGACAA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068589.1
CDS	83..2659
Translation	MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPATGKFSKSANSPDGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEETQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	NM_001075121.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation elongation factor 2 (EEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075121.1

ORF Insert Sequence:

ATGGTGAACT TCACAGTAGA CCAGATCCGG GCCATCATGG ACAAGAAGGC CAACATCCGG 
AACATGTCTG TCATTGCCCA CGTGGACCAT GGCAAATCCA CTCTGACGGA CTCCCTTGTG 
TGCAAGGCGG GTATCATCGC CTCTGCCCGG GCTGGGGAGA CCCGCTTCAC TGACACCCGG 
AAGGATGAGC AGGAGCGTTG CATCACCATC AAGTCAACGG CCATCTCCCT TTTCTACGAG 
CTTTCAGAGA ACGACTTGAA TTTCATCAAG CAGAGCAAGG ATGGCTCTGG CTTCCTCATC 
AACCTCATCG ACTCCCCTGG GCACGTCGAC TTTTCCTCAG AGGTGACAGC TGCACTCCGT 
GTCACTGACG GTGCCTTGGT GGTGGTGGAC TGCGTGTCGG GTGTGTGTGT GCAGACAGAG 
ACGGTGCTGC GCCAGGCCAT CGCTGAGCGC ATCAAGCCAG TGCTGATGAT GAACAAGATG 
GACCGGGCCC TGCTTGAGCT GCAGCTGGAG CCTGAGGAGC TCTACCAAAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAACGT CATCATCTCC ACTTACGGCG AGGGCGAGAG CGGCCCCATG 
GGCAACATCA TGATCGACCC CGTCCTTGGT ACTGTTGGCT TCGGGTCTGG TCTCCACGGT 
TGGGCCTTTA CCCTGAAGCA GTTTGCCGAG ATGTATGTGG CCAAGTTTGC TGCCAAGGGC 
GAGGGACAGC TAGGGCCTGC TGAGCGGGCC AAGAAGGTGG AGGACATGAT GAAAAAGCTG 
TGGGGAGACC GGTACTTTGA TCCAGCCACC GGCAAGTTCA GCAAGTCAGC CAACAGCCCT 
GATGGCAAGA AGCTGCCACG GACGTTCTGC CAGCTCATCC TGGACCCAAT CTTCAAGGTG 
TTTGATGCAA TCATGAACTT CAAGAAAGAG GAGACTGCAA AACTAATTGA AAAACTGGAC 
ATCAAGTTGG ACAGTGAAGA TAAGGACAAG GAGGGCAAAC CGCTTCTGAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCTGG GGATGCCCTG CTGCAGATGA TCACCATCCA CCTGCCTTCC 
CCTGTGACGG CCCAGAAGTA CCGCTGTGAG CTCCTGTACG AGGGGCCCCC AGACGATGAG 
GCAGCCATGG GCATTAAAAG CTGTGATCCC AAAGGCCCTC TCATGATGTA CATTTCCAAA 
ATGGTGCCGA CCTCTGACAA AGGTCGGTTC TATGCTTTCG GCCGGGTCTT CTCAGGGCTG 
GTGTCTACTG GCCTGAAGGT CCGCATCATG GGACCCAACT ATACTCCTGG GAAGAAGGAG 
GACCTGTACC TGAAGCCGAT CCAGAGGACA ATCCTGATGA TGGGCCGTTA CGTGGAGCCC 
ATCGAGGATG TGCCTTGTGG CAACATTGTG GGCCTGGTGG GCGTGGACCA GTTCCTGGTG 
AAGACTGGCA CCATCACCAC CTTTGAGCAT GCCCACAACA TGCGGGTGAT GAAGTTCAGT 
GTGAGCCCTG TTGTCAGGGT TGCTGTGGAG GCCAAGAACC CGGCCGACCT GCCCAAGCTG 
GTGGAAGGTC TGAAGCGGCT GGCCAAGTCG GACCCCATGG TGCAGTGCAT CATCGAGGAG 
TCCGGGGAGC ACATTATCGC GGGGGCTGGG GAGCTGCACT TGGAGATCTG CCTCAAGGAC 
CTGGAGGAGG ATCACGCTTG CATCCCCATC AAGAAATCTG ACCCAGTTGT CTCGTACCGG 
GAGACTGTCA GCGAGGAGTC AAACGTGCTC TGCCTATCCA AGTCCCCCAA CAAGCACAAT 
AGGCTTTATA TGAAGGCGCG GCCCTTCCCT GACGGCCTGG CTGAGGACAT CGACAAGGGC 
GAGGTGTCCG CCCGCCAGGA GCTCAAGCAG CGGGCCCGCT ACCTGGCTGA GAAGTACGAG 
TGGGATGTGG CCGAGGCCCG TAAGATCTGG TGCTTCGGCC CTGATGGCAC GGGCCCCAAC 
ATCCTCACGG ACATCACCAA GGGTGTGCAA TACCTCAATG AAATCAAGGA CAGTGTGGTA 
GCTGGCTTCC AGTGGGCCAC CAAAGAGGGG GCACTGTGCG AGGAGAACAT GCGGGGCGTG 
CGCTTTGACG TCCATGATGT GACGCTGCAC GCTGATGCCA TCCACCGTGG GGGTGGCCAG 
ATCATCCCCA CGGCTCGGCG CTGCCTGTAT GCCAGTGTGC TGACTGCCCA GCCCCGGCTC 
ATGGAGCCCA TCTATCTTGT GGAGATCCAG TGTCCAGAAC AAGTGGTTGG TGGCATCTAC 
GGTGTCCTGA ACAGGAAGCG GGGCCACGTC TTTGAGGAGA CCCAGGTGGC CGGCACCCCC 
ATGTTTGTTG TGAAGGCCTA CCTTCCTGTC AATGAGTCCT TTGGCTTCAC TGCTGACCTG 
AGGTCCAACA CGGGCGGCCA GGCCTTCCCC CAGTGTGTGT TCGACCACTG GCAGATCCTG 
CCCGGTGACC CCTTTGACAA CACCAGCCGC CCCAGTCAGG TAGTGGCGGA GACACGCAAG 
CGCAAAGGCC TGAAGGAAGG CATCCCAGCC CTGGACAACT TCCTGGACAA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Eukaryotic elongation factor-2 (eEF2): its regulation and peptide chain elongation.
Cell biochemistry and function29(3)227-34(2011 Apr)
Kaul G,Pattan G,Rafeequi T

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Localization of ZNF164, ZNF146, GGTA1, SOX2, PRLR and EEF2 on homoeologous cattle, sheep and goat chromosomes by fluorescent in situ hybridization and comparison with the human gene map.
Cytogenetics and cell genetics72(4)342-6(1996)
Hayes H,Le Chalony C,Goubin G,Mercier D,Payen E,Bignon C,Kohno K

Glucose induced genes in bovine aortic smooth muscle cells identified by mRNA differential display.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology8(1)103-6(1994 Jan)
Nishio Y,Aiello LP,King GL

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EEF2 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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