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CAPNS1 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol CAPNS1
Entrez Gene ID 281664
Full Name calpain small subunit 1
Synonyms CAPN4
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_024977941.1 XP_024833709.1 calpain small subunit 1 isoform X1
NM_174261.1 NP_776686.1 calpain small subunit 1

Pathways from BioSystems


Homologies


LOC712561 XP_002808235.1
Rattus norvegicus (Norway rat) LOC100911363 XP_003748884.1
Danio rerio (zebrafish) capns1a NP_001017899.2
Danio rerio (zebrafish) capns1b NP_001103176.1
Homo sapiens (human) CAPNS1 NP_001003962.1
Pan troglodytes (chimpanzee) CAPNS1 XP_003953505.1
Xenopus tropicalis (tropical clawed frog) capns2 NP_001016161.1
Canis lupus familiaris (dog) CAPNS1 XP_853634.1
Mus musculus (house mouse) Capns1 NP_033925.2
Rattus norvegicus (Norway rat) Capns1 NP_058814.1
Bos taurus (cattle) CAPNS1 NP_776686.1
Rattus norvegicus (Norway rat) LOC100912380 XP_003748875.2

Gene Ontology


Bibliography

Related articles in PubMed

A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene.
Juszczuk-Kubiak E, Flisikowski K, Wicińska K
Molecular biology reports37(1)473-6(2010 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Identification of major autolytic cleavage sites in the regulatory subunit of vascular calpain II. A comparison of partial amino-terminal sequences to deduced sequence from complementary DNA.
McCelland P, Lash JA, Hathaway DR
The Journal of biological chemistry264(29)17428-31(1989 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A new single nucleotide polymorphism was found in the bovine CAPNS1 gene exon 11 coding for the 3'UTR.
Title: A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene.

The following CAPNS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPNS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa224785 XM_024977941.1
Latest version!		 Bos taurus calpain small subunit 1 (CAPNS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OBa43499 NM_174261.1
Latest version!		 Bos taurus calpain small subunit 1 (CAPNS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $174.30
$249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa224785
Clone ID Related Accession (Same CDS sequence) XM_024977941.1
Accession Version XM_024977941.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 642bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product calpain small subunit 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGGGGCAA GGCTAGCCTT GTGCGCACGG CGTGGCCCTC GTAGTGAGGC GGCTGCGCAG 
TACAACCCAG AGCCCGTGCC CCCTCGCACA CATTACTCCA ACATTGAGGC CAATGAGAGT 
GAGGAGGTCC GGCAGTTCCG GAGGCTTTTT GCCCAGCTGG CTGGAGATGA CATGGAGGTC 
AGCGCCACAG AACTCATGAA CATTCTCAAC AAAGTGGTGA CCCGACATCC TGATCTGAAG 
ACTGATGGCT TTGGCATTGA CACATGTCGC AGCATGGTGG CTGTAATGGA TAGTGACACG 
ACTGGCAAAC TGGGCTTCGA AGAATTCAAG TACTTGTGGA ACAACATCAA AAAGTGGCAG 
GCCGTATACA AACAGTTTGA TGTTGACCGT TCAGGGACCA TTGGCAGCAG TGAACTCCCA 
GGGGCCTTTG AGGCAGCAGG ATTCCGCCTG AATGAACATC TCTACAACAT GATCATCCGA 
CGCTACTCAG ATGAGGGAGG GAACATGGAT TTTGACAATT TCATCAGCTG CCTGGTCAGA 
CTGGATGCCA TGTTCCGTGC CTTCAAATCT CTCGACAAAG ATGGCACTGG ACAAATCCAG 
GTGAACATCC AGGAGTGGCT GCAGCTGACC ATGTACTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024833709.1
CDS281..922
Translation

Target ORF information:

RefSeq Version XM_024977941.1
Organism Bos taurus(cattle)
Definition Bos taurus calpain small subunit 1 (CAPNS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024977941.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGGGGCAA GGCTAGCCTT GTGCGCACGG CGTGGCCCTC GTAGTGAGGC GGCTGCGCAG 
TACAACCCAG AGCCCGTGCC CCCTCGCACA CATTACTCCA ACATTGAGGC CAATGAGAGT 
GAGGAGGTCC GGCAGTTCCG GAGGCTTTTT GCCCAGCTGG CTGGAGATGA CATGGAGGTC 
AGCGCCACAG AACTCATGAA CATTCTCAAC AAAGTGGTGA CCCGACATCC TGATCTGAAG 
ACTGATGGCT TTGGCATTGA CACATGTCGC AGCATGGTGG CTGTAATGGA TAGTGACACG 
ACTGGCAAAC TGGGCTTCGA AGAATTCAAG TACTTGTGGA ACAACATCAA AAAGTGGCAG 
GCCGTATACA AACAGTTTGA TGTTGACCGT TCAGGGACCA TTGGCAGCAG TGAACTCCCA 
GGGGCCTTTG AGGCAGCAGG ATTCCGCCTG AATGAACATC TCTACAACAT GATCATCCGA 
CGCTACTCAG ATGAGGGAGG GAACATGGAT TTTGACAATT TCATCAGCTG CCTGGTCAGA 
CTGGATGCCA TGTTCCGTGC CTTCAAATCT CTCGACAAAG ATGGCACTGG ACAAATCCAG 
GTGAACATCC AGGAGTGGCT GCAGCTGACC ATGTACTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa43499
Clone ID Related Accession (Same CDS sequence) NM_174261.1
Accession Version NM_174261.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 792bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-25
Organism Bos taurus(cattle)
Product calpain small subunit 1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05065.1. Sequence Note: This sequence has been modified as follows: removed 28 bp suspected to be vector contamination from the 5' end. ##Evidence-Data-START## Transcript exon combination :: J05065.1, DV928241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCCTGG TTAACTCGTT CTTGAAGGGT GGCGGAGGCG GCGGAGGAGG CGGGGGCCTG 
GGCGGGGGTC TAGGGAATGT GCTTGGAGGC CTTATCAGCG GGGCTGGAGG CGGCGGCGGA 
GGCGGAGGCG GCGGCGGCGG TGGCGGCGGC GGAACCGCAA TGCGCATCCT GGGCGGGGTC 
ATTAGCGCCA TTAGTGAGGC GGCTGCCCAG TACAACCCAG AGCCCGTGCC CCCTCGCACA 
CATTACTCCA ACATTGAGGC CAATGAGAGT GAGGAGGTCC GGCAGTTCCG GAGGCTTTTT 
GCCCAGCTGG CTGGAGATGA CATGGAGGTC AGCGCCACAG AACTCATGAA CATTCTCAAC 
AAAGTGGTGA CCCGACATCC TGATCTGAAG ACTGATGGCT TTGGCATTGA CACATGTCGC 
AGCATGGTGG CTGTAATGGA TAGTGACACG ACTGGCAAAC TGGGCTTCGA AGAATTCAAG 
TACTTGTGGA ACAACATCAA AAAGTGGCAG GCCGTATACA AACAGTTTGA TGTTGACCGT 
TCAGGGACCA TTGGCAGCAG TGAACTCCCA GGGGCCTTTG AGGCAGCAGG ATTCCGCCTG 
AATGAACATC TCTACAACAT GATCATCCGA CGCTACTCAG ATGAGGGAGG GAACATGGAT 
TTTGACAATT TCATCAGCTG CCTGGTCAGA CTGGATGCCA TGTTCCGTGC CTTCAAATCT 
CTCGACAAAG ATGGCACTGG ACAAATCCAG GTGAACATCC AGGAGTGGCT GCAGCTGACC 
ATGTACTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_776686.1
CDS36..827
Translation

Target ORF information:

RefSeq Version NM_174261.1
Organism Bos taurus(cattle)
Definition Bos taurus calpain small subunit 1 (CAPNS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_174261.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCCTGG TTAACTCGTT CTTGAAGGGT GGCGGAGGCG GCGGAGGAGG CGGGGGCCTG 
GGCGGGGGTC TAGGGAATGT GCTTGGAGGC CTTATCAGCG GGGCTGGAGG CGGCGGCGGA 
GGCGGAGGCG GCGGCGGCGG TGGCGGCGGC GGAACCGCAA TGCGCATCCT GGGCGGGGTC 
ATTAGCGCCA TTAGTGAGGC GGCTGCCCAG TACAACCCAG AGCCCGTGCC CCCTCGCACA 
CATTACTCCA ACATTGAGGC CAATGAGAGT GAGGAGGTCC GGCAGTTCCG GAGGCTTTTT 
GCCCAGCTGG CTGGAGATGA CATGGAGGTC AGCGCCACAG AACTCATGAA CATTCTCAAC 
AAAGTGGTGA CCCGACATCC TGATCTGAAG ACTGATGGCT TTGGCATTGA CACATGTCGC 
AGCATGGTGG CTGTAATGGA TAGTGACACG ACTGGCAAAC TGGGCTTCGA AGAATTCAAG 
TACTTGTGGA ACAACATCAA AAAGTGGCAG GCCGTATACA AACAGTTTGA TGTTGACCGT 
TCAGGGACCA TTGGCAGCAG TGAACTCCCA GGGGCCTTTG AGGCAGCAGG ATTCCGCCTG 
AATGAACATC TCTACAACAT GATCATCCGA CGCTACTCAG ATGAGGGAGG GAACATGGAT 
TTTGACAATT TCATCAGCTG CCTGGTCAGA CTGGATGCCA TGTTCCGTGC CTTCAAATCT 
CTCGACAAAG ATGGCACTGG ACAAATCCAG GTGAACATCC AGGAGTGGCT GCAGCTGACC 
ATGTACTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A new SNP in the 3'UTR region of the bovine calpain small subunit (CAPNS1) gene.
Molecular biology reports37(1)473-6(2010 Jan)
Juszczuk-Kubiak E,Flisikowski K,Wicińska K


book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL


book

Identification of major autolytic cleavage sites in the regulatory subunit of vascular calpain II. A comparison of partial amino-terminal sequences to deduced sequence from complementary DNA.
The Journal of biological chemistry264(29)17428-31(1989 Oct)
McCelland P,Lash JA,Hathaway DR