IGF1R cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	IGF1R
Entrez Gene ID	281848
Full Name	insulin like growth factor 1 receptor
Synonyms	IGFR1
General protein information	Preferred Names insulin like growth factor 1 receptor Names insulin-like growth factor 1 receptor IGF-I receptor insulin-like growth factor I receptor insulin-like growth factor receptor type 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024981606.1	XP_024837374.1	insulin-like growth factor 1 receptor isoform X3
NM_001244612.1	NP_001231541.1	insulin-like growth factor 1 receptor precursor
XM_024981603.1	XP_024837371.1	insulin-like growth factor 1 receptor isoform X1
XM_024981604.1	XP_024837372.1	insulin-like growth factor 1 receptor isoform X1
XM_010816884.2	XP_010815186.2	insulin-like growth factor 1 receptor isoform X1
XM_024981605.1	XP_024837373.1	insulin-like growth factor 1 receptor isoform X2
XM_024981607.1	XP_024837375.1	insulin-like growth factor 1 receptor isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IGF1R	XP_510613.2
Canis lupus familiaris (dog)	IGF1R	XP_545828.2
Mus musculus (house mouse)	Igf1r	NP_034643.2
Gallus gallus (chicken)	IGF1R	NP_990363.1
Bos taurus (cattle)	IGF1R	NP_001231541.1
Danio rerio (zebrafish)	igf1ra	NP_694500.1
Caenorhabditis elegans (roundworm)	daf-2	NP_497650.4
Homo sapiens (human)	IGF1R	NP_000866.1
Rattus norvegicus (Norway rat)	Igf1r	NP_434694.1
Danio rerio (zebrafish)	igf1rb	NP_694501.1
Anopheles gambiae (African malaria mosquito)	INR	XP_320130.3
Xenopus tropicalis (tropical clawed frog)	igf1r	XP_002933351.1
	IGF1R	XP_001100407.1
Drosophila melanogaster (fruit fly)	InR	NP_001138093.1

Gene Ontology
Bibliography

Related articles in PubMed

Polymorphism in exon 2 encoding the putative ligand binding pocket of the bovine insulin-like growth factor 1 receptor affects milk traits in four different cattle breeds.
Szewczuk M
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie134(1)34-42(2017 Feb)

Periconceptional undernutrition modifies endocrine profiles and hepatic gene expression in sheep.
de Brun V, Meikle A, Casal A, Sequeira M, Contreras-Sol?s I, Carriquiry M, Forcada F, Sosa C, Abecia JA
Journal of animal physiology and animal nutrition99(4)710-8(2015 Aug)

Involvement of PAPP-A and IGFR1 in Cystic Ovarian Disease in Cattle.
Rodr?guez FM, Colombero M, Amweg AN, Huber E, Gareis NC, Salvetti NR, Ortega HH, Rey F
Reproduction in domestic animals = Zuchthygiene50(4)659-68(2015 Aug)

Effects in cattle of genetic variation within the IGF1R gene on the superovulation performance and pregnancy rates after embryo transfer.
Yang WC, Yang LG, Riaz H, Tang KQ, Chen L, Li SJ
Animal reproduction science143(1-4)24-9(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A total of 1169 cows of 4 pure breeds (Polish Holstein Friesian, Montbeliarde, Jersey and Holstein Friesian) were genotyped. The T (IGF1R/e2/MspI) and G (IGF1R/e2/TaqI) alleles were found to be prevalent. 3 combinations of genotypes (TT/GG, TT/AG and CT/GG) were associated with the highest productivity (milk, protein and fat yields) among the breeds studied, as opposed to individuals carrying the worst CC/AA combination.
Title: Polymorphism in exon 2 encoding the putative ligand binding pocket of the bovine insulin-like growth factor 1 receptor affects milk traits in four different cattle breeds.

study determined IGFR1 and PAPP-A expression both in follicles at different stages of development and in ovarian cysts; data indicate that animals with cystic ovarian disease (COD) have an altered regulation of the IGF system in the ovary and thus allow postulating IGFR1 expression and PAPP-A secretion as a modulator of IGF1 in cattle with COD
Title: Involvement of PAPP-A and IGFR1 in Cystic Ovarian Disease in Cattle.

Results of the present study showed, for the first time, that the polymorphism in IGF1R is associated with superovulation traits, and indicated that the IGFIR gene can be used as a potential marker for donor selection.
Title: Effects in cattle of genetic variation within the IGF1R gene on the superovulation performance and pregnancy rates after embryo transfer.

two SNPs in the coding region of the insulin-like growth factor 1 receptor gene may have a role in growth of Angus cattle
Title: Association of two SNPs in the coding region of the insulin-like growth factor 1 receptor (IGF1R) gene with growth-related traits in Angus cattle.

insulin-like growth factor (IGF) receptor glycosylation is critical to the maintenance of vascular smooth muscle phenotype in the presence of factors promoting osteogenic differentiation and mineralization
Title: Apposite insulin-like growth factor (IGF) receptor glycosylation is critical to the maintenance of vascular smooth muscle phenotype in the presence of factors promoting osteogenic differentiation and mineralization.

The following IGF1R gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IGF1R cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228210	XM_024981603.1 Latest version!	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa228210	XM_024981604.1 Latest version!	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa228211	XM_024981605.1 Latest version!	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa228212	XM_024981606.1 Latest version!	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa228213	XM_024981607.1 Latest version!	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa94164	XM_010816884.2 Latest version!	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa41254	NM_001244612.1 Latest version!	Bos taurus insulin like growth factor 1 receptor (IGF1R), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IGF1R ( XM_024981603.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837371.1 Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228210
Clone ID Related Accession (Same CDS sequence)	XM_024981603.1 , XM_024981604.1
Accession Version	XM_024981603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-like growth factor 1 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC TCAAGGACAT CGGGCTCTAC AACCTGCGGA ATATCACCCG TGGGGCCATC 
CGGATCGAGA AGAACGCCGA CCTGTGCTAC CTGTCCACGG TGGACTGGTC CCTGATCCTG 
GACGCGGCAT CCAACAACTA CATCGTGGGG AACAAGCCCC CCAAGGAGTG TGGGGACCTG 
TGCCCAGGGA CGATGGAGGA GAAGCCGCTG TGTGAGAAGA CGACCATCAA CAACGAGTAC 
AGCTACCGCT GCTGGACGAC AAACCGCTGC CAGAAAATGT GCCCCAGCGC GTGCGGGAAG 
CGGGCGTGTA CGGAGACCCA TGAGTGCTGC CACCCCGAGT GCCTGGGCAG CTGCAGCGCG 
CCCGACAACG CCACGGCCTG CGTGGCCTGC CGCCACTACT ACTACGCCGG CGTCTGCGTG 
CCCAGCTGCC CGCCCAACAC CTACCGCTTC GAGGGCTGGC GCTGCGTGGA CCGCGACTTC 
TGCGCCAACA TCCCCAACGC CGAGAGCAGC GACTCCGAGG GCTTCGTCAT CCACGACGGC 
GAGTGCATGC AGGAGTGCCC GTCGGGCTTC ATCCGCAACG GGAGCCAGAG CATGTACTGC 
ATCCCTTGCG AAGGCCCTTG TCCCAAAGTG TGTGAGGAAG AAAAGAAGAC AAAGACCATT 
GATTCTGTCA CTTCTGCTCA GATGCTCCAA GGATGCACCA TCTTCAAAGG CAATCTGCTC 
ATCAACATCC GACGCGGCAA CAACATCGCT TCGGAACTGG AGAACTTCAT GGGGCTCATC 
GAGGTGGTGA CGGGCTACGT GAAGATCCGC CACTCCCACG CCTTGGTCTC CTTGTCCTTC 
CTGAAGAACC TGCGCCAGAT CCTAGGGGAG GAGCAGCTAG AGGGGAACTA CTCCTTCTAC 
GTCCTTGACA ACCAGAACTT GCAGCAGCTG TGGGACTGGG ACCACCGCAA CCTGACCATC 
AAAGCTGGGA AAATGTACTT TGCGTTCAAT CCCAAATTGT GTGTCTCGGA GATTTACCGC 
ATGGAGGAAG TGACGGGGAC TAAAGGGCGC CAGAGCAAAG GGGACATAAA CACCAGGAAC 
AACGGGGAGA GAGCCTCCTG TGAAAGTGAC GTCCTGCACT TCACCTCCAC CACCACGTCG 
AAGAACCGAA TCATCATCAC CTGGCACCGG TACCGGCCCC CCGACTACCG GGACCTCATC 
AGCTTCACCG TCTACTACAA GGAAGCCCCC TTTAAAAACG TCACGGAGTA TGACGGGCAG 
GACGCCTGCG GCTCCAACAG CTGGAACATG GTGGACGTGG ACCTCCCGCC CAACAAGGAC 
GTGGAGCCTG GCATTTTGCT GCACGGGCTG AAGCCCTGGA CGCAGTACGC CGTTTACGTG 
AAGGCCGTGA CCCTCACCAT GGTGGAGAAC GACCACATCC GTGGGGCCAA GAGCGAGATC 
TTGTACATTC GCACCAACGC ATCAGTTCCT TCCATCCCCC TGGATGTCCT CTCGGCCTCA 
AACTCCTCTT CTCAGCTGAT CGTGAAGTGG AACCCGCCCT CCCTGCCCAA CGGCAACCTG 
AGCTACTACA TCGTGCGCTG GCAGCGCCAG CCCCAGGACA GCTACCTGTA CCGGCACAAC 
TACTGCTCCA AAGACAAAAT CCCCATCAGG AAGTACGCCG ACGGCACCAT CGACGTCGAG 
GAGGTGACGG AGAACCCCAA GACCGAGGTG TGCGGCGGGG AGAAAGGGCC GTGCTGCGCT 
TGTCCCAAAA CCGAAGCTGA GAAGCAGGCG GAGAAGGAGG AGGCCGAGTA CCGCAAGGTC 
TTCGAGAACT TCCTGCACAA CGCCATCTTC GTGCCCAGAC CCGAACGGAA GCGGAGGGAG 
GTCATGCAGA TCGCCAACAC CACCATGTCC AGCCGGAGCA GGAACACCAC GGTGCTGGAC 
ACCTACAACA TCACAGACCC GGAAGAGCTC GAGACCGAGT ACCCTTTCTT CGAGAGCAGA 
GTGGACAACA AGGAGAGAAC TGTCATCTCC AACCTCCGGC CTTTTACTCT GTACCGAATC 
GACATCCACA GCTGTAACCA TGAGGCTGAG AAGCTTGGCT GCAGCGCCTC CAACTTTGTC 
TTTGCAAGAA CCATGCCTGC AGAAGGAGCA GATGACATTC CTGGGCCAGT GACCTGGGAG 
CCAAGGCCTG AGAACTCCAT CTTTTTAAAA TGGCCAGAAC CTGAGAATCC CAATGGATTG 
ATCCTCATGT ATGAAATAAA ATATGGATCC CAAGTCGAGG ATCAGCGGGA ATGTGTGTCC 
AGACAGGAGT ACAGGAAGTA TGGAGGAGCC AAGCTAAACC GGCTCAACCC AGGGAACTAC 
ACAGCCCGGA TCCAGGCCAC CTCTCTCTCT GGGAATGGGT CGTGGACGGA TCCCGTGTTC 
TTCTACGTTC AGGCCAAGAC GACATATGAG AACTTCATCC ACCTGATGAT CGCTCTGCCC 
ATTGCGGTTC TGTTGATAGT GGGAGGCTTG GTTATAATGC TGTACGTCTT CCATAGAAAA 
AGAAATAGCA GCAGGCTGGG AAATGGAGTG CTGTATGCCT CTGTGAACCC GGAGTATTTC 
AGCGCAGCTG ATGTGTACGT TCCTGACGAG TGGGAGGTGG CCCGGGAGAA GATCACCATG 
AGCCGCGAGC TGGGCCAGGG CTCCTTCGGG ATGGTCTACG AAGGCGTGGC CAAGGGCGTG 
GTCAAGGATG AGCCCGAGAC CAGGGTGGCC ATTAAGACGG TGAACGAGGC CGCCAGCATG 
CGCGAGCGGA TCGAGTTTCT CAATGAGGCC TCGGTGATGA AGGAGTTCAA TTGTCACCAC 
GTGGTGCGCT TGCTGGGCGT GGTGTCCCAG GGCCAGCCGA CGCTGGTCAT CATGGAACTG 
ATGACCCGGG GGGACCTCAA GAGTTATCTC CGGTCTCTGA GGCCAGAAAT GGAGAATAAT 
CCAGTCCTAG CACCTCCCAG CCTAAGCAAA ATGATCCAGA TGGCTGGTGA GATTGCAGAT 
GGCATGGCAT ACCTCAACGC CAATAAGTTT GTCCACAGAG ACCTCGCTGC CCGGAACTGC 
ATGGTGGCCG AAGATTTCAC AGTCAAAATC GGAGATTTTG GGATGACGAG AGACATCTAT 
GAGACGGACT ATTACCGGAA GGGGGGGAAG GGGCTGCTGC CCGTCCGCTG GATGTCCCCT 
GAGTCCCTCA AGGACGGAGT CTTCACCACT CACTCTGACG TCTGGTCCTT CGGGGTCGTC 
CTCTGGGAGA TCGCCACGCT GGCTGAGCAG CCGTATCAGG GCCTGTCCAA TGAGCAGGTC 
CTTCGCTTCG TCATGGAGGG CGGCCTTCTG GACAAGCCGG ACAACTGTCC TGACATGCTG 
TTTGAGCTGA TGCGCATGTG CTGGCAGTAC AACCCCAAGA TGCGGCCTTC GTTCCTGGAG 
ATCATCAGCA GCGTCAAGGA CGAGATGGAG GCGGGCTTCC GGGAGGTCTC CTTCTACTAC 
AGCGAGGAGA ACAAGCCGCC GGAGCCGGAG GAGCTGGACC TGGAGCCCGA GAACATGGAG 
AGCGTGCCGC TGGACCCCTC GGCCTCCTCG GCCTCCCTGC CGCTGCCCGA CAGACACTCA 
GGACACAAGG CCGAGAACGG CCCGGGCCCG GGCGTGCTGG TCCTGCGGGC CAGCTTCGAC 
GAGAGGCAGC CCTACGCGCA CATGAACGGG GGCCGCAAGA ACGAGCGCGC CCTGCCGCTG 
CCCCAGTCCT CCACCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837371.1
CDS	419..4159
Translation	MTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAASNNYIVGNKPPKECGDLCPGTMEEKPLCEKTTINNEYSYRCWTTNRCQKMCPSACGKRACTETHECCHPECLGSCSAPDNATACVACRHYYYAGVCVPSCPPNTYRFEGWRCVDRDFCANIPNAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRQILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDSYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNAIFVPRPERKRREVMQIANTTMSSRSRNTTVLDTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTTYENFIHLMIALPIAVLLIVGGLVIMLYVFHRKRNSSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSVKDEMEAGFREVSFYYSEENKPPEPEELDLEPENMESVPLDPSASSASLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC

Target ORF information:

RefSeq Version	XM_024981603.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981603.1

ORF Insert Sequence:

ATGACCAACC TCAAGGACAT CGGGCTCTAC AACCTGCGGA ATATCACCCG TGGGGCCATC 
CGGATCGAGA AGAACGCCGA CCTGTGCTAC CTGTCCACGG TGGACTGGTC CCTGATCCTG 
GACGCGGCAT CCAACAACTA CATCGTGGGG AACAAGCCCC CCAAGGAGTG TGGGGACCTG 
TGCCCAGGGA CGATGGAGGA GAAGCCGCTG TGTGAGAAGA CGACCATCAA CAACGAGTAC 
AGCTACCGCT GCTGGACGAC AAACCGCTGC CAGAAAATGT GCCCCAGCGC GTGCGGGAAG 
CGGGCGTGTA CGGAGACCCA TGAGTGCTGC CACCCCGAGT GCCTGGGCAG CTGCAGCGCG 
CCCGACAACG CCACGGCCTG CGTGGCCTGC CGCCACTACT ACTACGCCGG CGTCTGCGTG 
CCCAGCTGCC CGCCCAACAC CTACCGCTTC GAGGGCTGGC GCTGCGTGGA CCGCGACTTC 
TGCGCCAACA TCCCCAACGC CGAGAGCAGC GACTCCGAGG GCTTCGTCAT CCACGACGGC 
GAGTGCATGC AGGAGTGCCC GTCGGGCTTC ATCCGCAACG GGAGCCAGAG CATGTACTGC 
ATCCCTTGCG AAGGCCCTTG TCCCAAAGTG TGTGAGGAAG AAAAGAAGAC AAAGACCATT 
GATTCTGTCA CTTCTGCTCA GATGCTCCAA GGATGCACCA TCTTCAAAGG CAATCTGCTC 
ATCAACATCC GACGCGGCAA CAACATCGCT TCGGAACTGG AGAACTTCAT GGGGCTCATC 
GAGGTGGTGA CGGGCTACGT GAAGATCCGC CACTCCCACG CCTTGGTCTC CTTGTCCTTC 
CTGAAGAACC TGCGCCAGAT CCTAGGGGAG GAGCAGCTAG AGGGGAACTA CTCCTTCTAC 
GTCCTTGACA ACCAGAACTT GCAGCAGCTG TGGGACTGGG ACCACCGCAA CCTGACCATC 
AAAGCTGGGA AAATGTACTT TGCGTTCAAT CCCAAATTGT GTGTCTCGGA GATTTACCGC 
ATGGAGGAAG TGACGGGGAC TAAAGGGCGC CAGAGCAAAG GGGACATAAA CACCAGGAAC 
AACGGGGAGA GAGCCTCCTG TGAAAGTGAC GTCCTGCACT TCACCTCCAC CACCACGTCG 
AAGAACCGAA TCATCATCAC CTGGCACCGG TACCGGCCCC CCGACTACCG GGACCTCATC 
AGCTTCACCG TCTACTACAA GGAAGCCCCC TTTAAAAACG TCACGGAGTA TGACGGGCAG 
GACGCCTGCG GCTCCAACAG CTGGAACATG GTGGACGTGG ACCTCCCGCC CAACAAGGAC 
GTGGAGCCTG GCATTTTGCT GCACGGGCTG AAGCCCTGGA CGCAGTACGC CGTTTACGTG 
AAGGCCGTGA CCCTCACCAT GGTGGAGAAC GACCACATCC GTGGGGCCAA GAGCGAGATC 
TTGTACATTC GCACCAACGC ATCAGTTCCT TCCATCCCCC TGGATGTCCT CTCGGCCTCA 
AACTCCTCTT CTCAGCTGAT CGTGAAGTGG AACCCGCCCT CCCTGCCCAA CGGCAACCTG 
AGCTACTACA TCGTGCGCTG GCAGCGCCAG CCCCAGGACA GCTACCTGTA CCGGCACAAC 
TACTGCTCCA AAGACAAAAT CCCCATCAGG AAGTACGCCG ACGGCACCAT CGACGTCGAG 
GAGGTGACGG AGAACCCCAA GACCGAGGTG TGCGGCGGGG AGAAAGGGCC GTGCTGCGCT 
TGTCCCAAAA CCGAAGCTGA GAAGCAGGCG GAGAAGGAGG AGGCCGAGTA CCGCAAGGTC 
TTCGAGAACT TCCTGCACAA CGCCATCTTC GTGCCCAGAC CCGAACGGAA GCGGAGGGAG 
GTCATGCAGA TCGCCAACAC CACCATGTCC AGCCGGAGCA GGAACACCAC GGTGCTGGAC 
ACCTACAACA TCACAGACCC GGAAGAGCTC GAGACCGAGT ACCCTTTCTT CGAGAGCAGA 
GTGGACAACA AGGAGAGAAC TGTCATCTCC AACCTCCGGC CTTTTACTCT GTACCGAATC 
GACATCCACA GCTGTAACCA TGAGGCTGAG AAGCTTGGCT GCAGCGCCTC CAACTTTGTC 
TTTGCAAGAA CCATGCCTGC AGAAGGAGCA GATGACATTC CTGGGCCAGT GACCTGGGAG 
CCAAGGCCTG AGAACTCCAT CTTTTTAAAA TGGCCAGAAC CTGAGAATCC CAATGGATTG 
ATCCTCATGT ATGAAATAAA ATATGGATCC CAAGTCGAGG ATCAGCGGGA ATGTGTGTCC 
AGACAGGAGT ACAGGAAGTA TGGAGGAGCC AAGCTAAACC GGCTCAACCC AGGGAACTAC 
ACAGCCCGGA TCCAGGCCAC CTCTCTCTCT GGGAATGGGT CGTGGACGGA TCCCGTGTTC 
TTCTACGTTC AGGCCAAGAC GACATATGAG AACTTCATCC ACCTGATGAT CGCTCTGCCC 
ATTGCGGTTC TGTTGATAGT GGGAGGCTTG GTTATAATGC TGTACGTCTT CCATAGAAAA 
AGAAATAGCA GCAGGCTGGG AAATGGAGTG CTGTATGCCT CTGTGAACCC GGAGTATTTC 
AGCGCAGCTG ATGTGTACGT TCCTGACGAG TGGGAGGTGG CCCGGGAGAA GATCACCATG 
AGCCGCGAGC TGGGCCAGGG CTCCTTCGGG ATGGTCTACG AAGGCGTGGC CAAGGGCGTG 
GTCAAGGATG AGCCCGAGAC CAGGGTGGCC ATTAAGACGG TGAACGAGGC CGCCAGCATG 
CGCGAGCGGA TCGAGTTTCT CAATGAGGCC TCGGTGATGA AGGAGTTCAA TTGTCACCAC 
GTGGTGCGCT TGCTGGGCGT GGTGTCCCAG GGCCAGCCGA CGCTGGTCAT CATGGAACTG 
ATGACCCGGG GGGACCTCAA GAGTTATCTC CGGTCTCTGA GGCCAGAAAT GGAGAATAAT 
CCAGTCCTAG CACCTCCCAG CCTAAGCAAA ATGATCCAGA TGGCTGGTGA GATTGCAGAT 
GGCATGGCAT ACCTCAACGC CAATAAGTTT GTCCACAGAG ACCTCGCTGC CCGGAACTGC 
ATGGTGGCCG AAGATTTCAC AGTCAAAATC GGAGATTTTG GGATGACGAG AGACATCTAT 
GAGACGGACT ATTACCGGAA GGGGGGGAAG GGGCTGCTGC CCGTCCGCTG GATGTCCCCT 
GAGTCCCTCA AGGACGGAGT CTTCACCACT CACTCTGACG TCTGGTCCTT CGGGGTCGTC 
CTCTGGGAGA TCGCCACGCT GGCTGAGCAG CCGTATCAGG GCCTGTCCAA TGAGCAGGTC 
CTTCGCTTCG TCATGGAGGG CGGCCTTCTG GACAAGCCGG ACAACTGTCC TGACATGCTG 
TTTGAGCTGA TGCGCATGTG CTGGCAGTAC AACCCCAAGA TGCGGCCTTC GTTCCTGGAG 
ATCATCAGCA GCGTCAAGGA CGAGATGGAG GCGGGCTTCC GGGAGGTCTC CTTCTACTAC 
AGCGAGGAGA ACAAGCCGCC GGAGCCGGAG GAGCTGGACC TGGAGCCCGA GAACATGGAG 
AGCGTGCCGC TGGACCCCTC GGCCTCCTCG GCCTCCCTGC CGCTGCCCGA CAGACACTCA 
GGACACAAGG CCGAGAACGG CCCGGGCCCG GGCGTGCTGG TCCTGCGGGC CAGCTTCGAC 
GAGAGGCAGC CCTACGCGCA CATGAACGGG GGCCGCAAGA ACGAGCGCGC CCTGCCGCTG 
CCCCAGTCCT CCACCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF1R ( XM_024981604.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837372.1 Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa228210
Clone ID Related Accession (Same CDS sequence)	XM_024981603.1 , XM_024981604.1
Accession Version	XM_024981604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-like growth factor 1 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC TCAAGGACAT CGGGCTCTAC AACCTGCGGA ATATCACCCG TGGGGCCATC 
CGGATCGAGA AGAACGCCGA CCTGTGCTAC CTGTCCACGG TGGACTGGTC CCTGATCCTG 
GACGCGGCAT CCAACAACTA CATCGTGGGG AACAAGCCCC CCAAGGAGTG TGGGGACCTG 
TGCCCAGGGA CGATGGAGGA GAAGCCGCTG TGTGAGAAGA CGACCATCAA CAACGAGTAC 
AGCTACCGCT GCTGGACGAC AAACCGCTGC CAGAAAATGT GCCCCAGCGC GTGCGGGAAG 
CGGGCGTGTA CGGAGACCCA TGAGTGCTGC CACCCCGAGT GCCTGGGCAG CTGCAGCGCG 
CCCGACAACG CCACGGCCTG CGTGGCCTGC CGCCACTACT ACTACGCCGG CGTCTGCGTG 
CCCAGCTGCC CGCCCAACAC CTACCGCTTC GAGGGCTGGC GCTGCGTGGA CCGCGACTTC 
TGCGCCAACA TCCCCAACGC CGAGAGCAGC GACTCCGAGG GCTTCGTCAT CCACGACGGC 
GAGTGCATGC AGGAGTGCCC GTCGGGCTTC ATCCGCAACG GGAGCCAGAG CATGTACTGC 
ATCCCTTGCG AAGGCCCTTG TCCCAAAGTG TGTGAGGAAG AAAAGAAGAC AAAGACCATT 
GATTCTGTCA CTTCTGCTCA GATGCTCCAA GGATGCACCA TCTTCAAAGG CAATCTGCTC 
ATCAACATCC GACGCGGCAA CAACATCGCT TCGGAACTGG AGAACTTCAT GGGGCTCATC 
GAGGTGGTGA CGGGCTACGT GAAGATCCGC CACTCCCACG CCTTGGTCTC CTTGTCCTTC 
CTGAAGAACC TGCGCCAGAT CCTAGGGGAG GAGCAGCTAG AGGGGAACTA CTCCTTCTAC 
GTCCTTGACA ACCAGAACTT GCAGCAGCTG TGGGACTGGG ACCACCGCAA CCTGACCATC 
AAAGCTGGGA AAATGTACTT TGCGTTCAAT CCCAAATTGT GTGTCTCGGA GATTTACCGC 
ATGGAGGAAG TGACGGGGAC TAAAGGGCGC CAGAGCAAAG GGGACATAAA CACCAGGAAC 
AACGGGGAGA GAGCCTCCTG TGAAAGTGAC GTCCTGCACT TCACCTCCAC CACCACGTCG 
AAGAACCGAA TCATCATCAC CTGGCACCGG TACCGGCCCC CCGACTACCG GGACCTCATC 
AGCTTCACCG TCTACTACAA GGAAGCCCCC TTTAAAAACG TCACGGAGTA TGACGGGCAG 
GACGCCTGCG GCTCCAACAG CTGGAACATG GTGGACGTGG ACCTCCCGCC CAACAAGGAC 
GTGGAGCCTG GCATTTTGCT GCACGGGCTG AAGCCCTGGA CGCAGTACGC CGTTTACGTG 
AAGGCCGTGA CCCTCACCAT GGTGGAGAAC GACCACATCC GTGGGGCCAA GAGCGAGATC 
TTGTACATTC GCACCAACGC ATCAGTTCCT TCCATCCCCC TGGATGTCCT CTCGGCCTCA 
AACTCCTCTT CTCAGCTGAT CGTGAAGTGG AACCCGCCCT CCCTGCCCAA CGGCAACCTG 
AGCTACTACA TCGTGCGCTG GCAGCGCCAG CCCCAGGACA GCTACCTGTA CCGGCACAAC 
TACTGCTCCA AAGACAAAAT CCCCATCAGG AAGTACGCCG ACGGCACCAT CGACGTCGAG 
GAGGTGACGG AGAACCCCAA GACCGAGGTG TGCGGCGGGG AGAAAGGGCC GTGCTGCGCT 
TGTCCCAAAA CCGAAGCTGA GAAGCAGGCG GAGAAGGAGG AGGCCGAGTA CCGCAAGGTC 
TTCGAGAACT TCCTGCACAA CGCCATCTTC GTGCCCAGAC CCGAACGGAA GCGGAGGGAG 
GTCATGCAGA TCGCCAACAC CACCATGTCC AGCCGGAGCA GGAACACCAC GGTGCTGGAC 
ACCTACAACA TCACAGACCC GGAAGAGCTC GAGACCGAGT ACCCTTTCTT CGAGAGCAGA 
GTGGACAACA AGGAGAGAAC TGTCATCTCC AACCTCCGGC CTTTTACTCT GTACCGAATC 
GACATCCACA GCTGTAACCA TGAGGCTGAG AAGCTTGGCT GCAGCGCCTC CAACTTTGTC 
TTTGCAAGAA CCATGCCTGC AGAAGGAGCA GATGACATTC CTGGGCCAGT GACCTGGGAG 
CCAAGGCCTG AGAACTCCAT CTTTTTAAAA TGGCCAGAAC CTGAGAATCC CAATGGATTG 
ATCCTCATGT ATGAAATAAA ATATGGATCC CAAGTCGAGG ATCAGCGGGA ATGTGTGTCC 
AGACAGGAGT ACAGGAAGTA TGGAGGAGCC AAGCTAAACC GGCTCAACCC AGGGAACTAC 
ACAGCCCGGA TCCAGGCCAC CTCTCTCTCT GGGAATGGGT CGTGGACGGA TCCCGTGTTC 
TTCTACGTTC AGGCCAAGAC GACATATGAG AACTTCATCC ACCTGATGAT CGCTCTGCCC 
ATTGCGGTTC TGTTGATAGT GGGAGGCTTG GTTATAATGC TGTACGTCTT CCATAGAAAA 
AGAAATAGCA GCAGGCTGGG AAATGGAGTG CTGTATGCCT CTGTGAACCC GGAGTATTTC 
AGCGCAGCTG ATGTGTACGT TCCTGACGAG TGGGAGGTGG CCCGGGAGAA GATCACCATG 
AGCCGCGAGC TGGGCCAGGG CTCCTTCGGG ATGGTCTACG AAGGCGTGGC CAAGGGCGTG 
GTCAAGGATG AGCCCGAGAC CAGGGTGGCC ATTAAGACGG TGAACGAGGC CGCCAGCATG 
CGCGAGCGGA TCGAGTTTCT CAATGAGGCC TCGGTGATGA AGGAGTTCAA TTGTCACCAC 
GTGGTGCGCT TGCTGGGCGT GGTGTCCCAG GGCCAGCCGA CGCTGGTCAT CATGGAACTG 
ATGACCCGGG GGGACCTCAA GAGTTATCTC CGGTCTCTGA GGCCAGAAAT GGAGAATAAT 
CCAGTCCTAG CACCTCCCAG CCTAAGCAAA ATGATCCAGA TGGCTGGTGA GATTGCAGAT 
GGCATGGCAT ACCTCAACGC CAATAAGTTT GTCCACAGAG ACCTCGCTGC CCGGAACTGC 
ATGGTGGCCG AAGATTTCAC AGTCAAAATC GGAGATTTTG GGATGACGAG AGACATCTAT 
GAGACGGACT ATTACCGGAA GGGGGGGAAG GGGCTGCTGC CCGTCCGCTG GATGTCCCCT 
GAGTCCCTCA AGGACGGAGT CTTCACCACT CACTCTGACG TCTGGTCCTT CGGGGTCGTC 
CTCTGGGAGA TCGCCACGCT GGCTGAGCAG CCGTATCAGG GCCTGTCCAA TGAGCAGGTC 
CTTCGCTTCG TCATGGAGGG CGGCCTTCTG GACAAGCCGG ACAACTGTCC TGACATGCTG 
TTTGAGCTGA TGCGCATGTG CTGGCAGTAC AACCCCAAGA TGCGGCCTTC GTTCCTGGAG 
ATCATCAGCA GCGTCAAGGA CGAGATGGAG GCGGGCTTCC GGGAGGTCTC CTTCTACTAC 
AGCGAGGAGA ACAAGCCGCC GGAGCCGGAG GAGCTGGACC TGGAGCCCGA GAACATGGAG 
AGCGTGCCGC TGGACCCCTC GGCCTCCTCG GCCTCCCTGC CGCTGCCCGA CAGACACTCA 
GGACACAAGG CCGAGAACGG CCCGGGCCCG GGCGTGCTGG TCCTGCGGGC CAGCTTCGAC 
GAGAGGCAGC CCTACGCGCA CATGAACGGG GGCCGCAAGA ACGAGCGCGC CCTGCCGCTG 
CCCCAGTCCT CCACCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837372.1
CDS	288..4028
Translation	MTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAASNNYIVGNKPPKECGDLCPGTMEEKPLCEKTTINNEYSYRCWTTNRCQKMCPSACGKRACTETHECCHPECLGSCSAPDNATACVACRHYYYAGVCVPSCPPNTYRFEGWRCVDRDFCANIPNAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRQILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDSYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNAIFVPRPERKRREVMQIANTTMSSRSRNTTVLDTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTTYENFIHLMIALPIAVLLIVGGLVIMLYVFHRKRNSSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSVKDEMEAGFREVSFYYSEENKPPEPEELDLEPENMESVPLDPSASSASLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC

Target ORF information:

RefSeq Version	XM_024981604.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981604.1

ORF Insert Sequence:

ATGACCAACC TCAAGGACAT CGGGCTCTAC AACCTGCGGA ATATCACCCG TGGGGCCATC 
CGGATCGAGA AGAACGCCGA CCTGTGCTAC CTGTCCACGG TGGACTGGTC CCTGATCCTG 
GACGCGGCAT CCAACAACTA CATCGTGGGG AACAAGCCCC CCAAGGAGTG TGGGGACCTG 
TGCCCAGGGA CGATGGAGGA GAAGCCGCTG TGTGAGAAGA CGACCATCAA CAACGAGTAC 
AGCTACCGCT GCTGGACGAC AAACCGCTGC CAGAAAATGT GCCCCAGCGC GTGCGGGAAG 
CGGGCGTGTA CGGAGACCCA TGAGTGCTGC CACCCCGAGT GCCTGGGCAG CTGCAGCGCG 
CCCGACAACG CCACGGCCTG CGTGGCCTGC CGCCACTACT ACTACGCCGG CGTCTGCGTG 
CCCAGCTGCC CGCCCAACAC CTACCGCTTC GAGGGCTGGC GCTGCGTGGA CCGCGACTTC 
TGCGCCAACA TCCCCAACGC CGAGAGCAGC GACTCCGAGG GCTTCGTCAT CCACGACGGC 
GAGTGCATGC AGGAGTGCCC GTCGGGCTTC ATCCGCAACG GGAGCCAGAG CATGTACTGC 
ATCCCTTGCG AAGGCCCTTG TCCCAAAGTG TGTGAGGAAG AAAAGAAGAC AAAGACCATT 
GATTCTGTCA CTTCTGCTCA GATGCTCCAA GGATGCACCA TCTTCAAAGG CAATCTGCTC 
ATCAACATCC GACGCGGCAA CAACATCGCT TCGGAACTGG AGAACTTCAT GGGGCTCATC 
GAGGTGGTGA CGGGCTACGT GAAGATCCGC CACTCCCACG CCTTGGTCTC CTTGTCCTTC 
CTGAAGAACC TGCGCCAGAT CCTAGGGGAG GAGCAGCTAG AGGGGAACTA CTCCTTCTAC 
GTCCTTGACA ACCAGAACTT GCAGCAGCTG TGGGACTGGG ACCACCGCAA CCTGACCATC 
AAAGCTGGGA AAATGTACTT TGCGTTCAAT CCCAAATTGT GTGTCTCGGA GATTTACCGC 
ATGGAGGAAG TGACGGGGAC TAAAGGGCGC CAGAGCAAAG GGGACATAAA CACCAGGAAC 
AACGGGGAGA GAGCCTCCTG TGAAAGTGAC GTCCTGCACT TCACCTCCAC CACCACGTCG 
AAGAACCGAA TCATCATCAC CTGGCACCGG TACCGGCCCC CCGACTACCG GGACCTCATC 
AGCTTCACCG TCTACTACAA GGAAGCCCCC TTTAAAAACG TCACGGAGTA TGACGGGCAG 
GACGCCTGCG GCTCCAACAG CTGGAACATG GTGGACGTGG ACCTCCCGCC CAACAAGGAC 
GTGGAGCCTG GCATTTTGCT GCACGGGCTG AAGCCCTGGA CGCAGTACGC CGTTTACGTG 
AAGGCCGTGA CCCTCACCAT GGTGGAGAAC GACCACATCC GTGGGGCCAA GAGCGAGATC 
TTGTACATTC GCACCAACGC ATCAGTTCCT TCCATCCCCC TGGATGTCCT CTCGGCCTCA 
AACTCCTCTT CTCAGCTGAT CGTGAAGTGG AACCCGCCCT CCCTGCCCAA CGGCAACCTG 
AGCTACTACA TCGTGCGCTG GCAGCGCCAG CCCCAGGACA GCTACCTGTA CCGGCACAAC 
TACTGCTCCA AAGACAAAAT CCCCATCAGG AAGTACGCCG ACGGCACCAT CGACGTCGAG 
GAGGTGACGG AGAACCCCAA GACCGAGGTG TGCGGCGGGG AGAAAGGGCC GTGCTGCGCT 
TGTCCCAAAA CCGAAGCTGA GAAGCAGGCG GAGAAGGAGG AGGCCGAGTA CCGCAAGGTC 
TTCGAGAACT TCCTGCACAA CGCCATCTTC GTGCCCAGAC CCGAACGGAA GCGGAGGGAG 
GTCATGCAGA TCGCCAACAC CACCATGTCC AGCCGGAGCA GGAACACCAC GGTGCTGGAC 
ACCTACAACA TCACAGACCC GGAAGAGCTC GAGACCGAGT ACCCTTTCTT CGAGAGCAGA 
GTGGACAACA AGGAGAGAAC TGTCATCTCC AACCTCCGGC CTTTTACTCT GTACCGAATC 
GACATCCACA GCTGTAACCA TGAGGCTGAG AAGCTTGGCT GCAGCGCCTC CAACTTTGTC 
TTTGCAAGAA CCATGCCTGC AGAAGGAGCA GATGACATTC CTGGGCCAGT GACCTGGGAG 
CCAAGGCCTG AGAACTCCAT CTTTTTAAAA TGGCCAGAAC CTGAGAATCC CAATGGATTG 
ATCCTCATGT ATGAAATAAA ATATGGATCC CAAGTCGAGG ATCAGCGGGA ATGTGTGTCC 
AGACAGGAGT ACAGGAAGTA TGGAGGAGCC AAGCTAAACC GGCTCAACCC AGGGAACTAC 
ACAGCCCGGA TCCAGGCCAC CTCTCTCTCT GGGAATGGGT CGTGGACGGA TCCCGTGTTC 
TTCTACGTTC AGGCCAAGAC GACATATGAG AACTTCATCC ACCTGATGAT CGCTCTGCCC 
ATTGCGGTTC TGTTGATAGT GGGAGGCTTG GTTATAATGC TGTACGTCTT CCATAGAAAA 
AGAAATAGCA GCAGGCTGGG AAATGGAGTG CTGTATGCCT CTGTGAACCC GGAGTATTTC 
AGCGCAGCTG ATGTGTACGT TCCTGACGAG TGGGAGGTGG CCCGGGAGAA GATCACCATG 
AGCCGCGAGC TGGGCCAGGG CTCCTTCGGG ATGGTCTACG AAGGCGTGGC CAAGGGCGTG 
GTCAAGGATG AGCCCGAGAC CAGGGTGGCC ATTAAGACGG TGAACGAGGC CGCCAGCATG 
CGCGAGCGGA TCGAGTTTCT CAATGAGGCC TCGGTGATGA AGGAGTTCAA TTGTCACCAC 
GTGGTGCGCT TGCTGGGCGT GGTGTCCCAG GGCCAGCCGA CGCTGGTCAT CATGGAACTG 
ATGACCCGGG GGGACCTCAA GAGTTATCTC CGGTCTCTGA GGCCAGAAAT GGAGAATAAT 
CCAGTCCTAG CACCTCCCAG CCTAAGCAAA ATGATCCAGA TGGCTGGTGA GATTGCAGAT 
GGCATGGCAT ACCTCAACGC CAATAAGTTT GTCCACAGAG ACCTCGCTGC CCGGAACTGC 
ATGGTGGCCG AAGATTTCAC AGTCAAAATC GGAGATTTTG GGATGACGAG AGACATCTAT 
GAGACGGACT ATTACCGGAA GGGGGGGAAG GGGCTGCTGC CCGTCCGCTG GATGTCCCCT 
GAGTCCCTCA AGGACGGAGT CTTCACCACT CACTCTGACG TCTGGTCCTT CGGGGTCGTC 
CTCTGGGAGA TCGCCACGCT GGCTGAGCAG CCGTATCAGG GCCTGTCCAA TGAGCAGGTC 
CTTCGCTTCG TCATGGAGGG CGGCCTTCTG GACAAGCCGG ACAACTGTCC TGACATGCTG 
TTTGAGCTGA TGCGCATGTG CTGGCAGTAC AACCCCAAGA TGCGGCCTTC GTTCCTGGAG 
ATCATCAGCA GCGTCAAGGA CGAGATGGAG GCGGGCTTCC GGGAGGTCTC CTTCTACTAC 
AGCGAGGAGA ACAAGCCGCC GGAGCCGGAG GAGCTGGACC TGGAGCCCGA GAACATGGAG 
AGCGTGCCGC TGGACCCCTC GGCCTCCTCG GCCTCCCTGC CGCTGCCCGA CAGACACTCA 
GGACACAAGG CCGAGAACGG CCCGGGCCCG GGCGTGCTGG TCCTGCGGGC CAGCTTCGAC 
GAGAGGCAGC CCTACGCGCA CATGAACGGG GGCCGCAAGA ACGAGCGCGC CCTGCCGCTG 
CCCCAGTCCT CCACCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF1R ( XM_024981605.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837373.1 Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X3, mRNA.

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CloneID	OBa228211
Clone ID Related Accession (Same CDS sequence)	XM_024981605.1
Accession Version	XM_024981605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-like growth factor 1 receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAACC CTGCTCACCT GGCTCGAAAT GCAGAGTACT TGCTCCCTGC CCCAGCGAAA 
TTCTCAACAT GTAACACTGT GTGCCCCAGC GCGTGCGGGA AGCGGGCGTG TACGGAGACC 
CATGAGTGCT GCCACCCCGA GTGCCTGGGC AGCTGCAGCG CGCCCGACAA CGCCACGGCC 
TGCGTGGCCT GCCGCCACTA CTACTACGCC GGCGTCTGCG TGCCCAGCTG CCCGCCCAAC 
ACCTACCGCT TCGAGGGCTG GCGCTGCGTG GACCGCGACT TCTGCGCCAA CATCCCCAAC 
GCCGAGAGCA GCGACTCCGA GGGCTTCGTC ATCCACGACG GCGAGTGCAT GCAGGAGTGC 
CCGTCGGGCT TCATCCGCAA CGGGAGCCAG AGCATGTACT GCATCCCTTG CGAAGGCCCT 
TGTCCCAAAG TGTGTGAGGA AGAAAAGAAG ACAAAGACCA TTGATTCTGT CACTTCTGCT 
CAGATGCTCC AAGGATGCAC CATCTTCAAA GGCAATCTGC TCATCAACAT CCGACGCGGC 
AACAACATCG CTTCGGAACT GGAGAACTTC ATGGGGCTCA TCGAGGTGGT GACGGGCTAC 
GTGAAGATCC GCCACTCCCA CGCCTTGGTC TCCTTGTCCT TCCTGAAGAA CCTGCGCCAG 
ATCCTAGGGG AGGAGCAGCT AGAGGGGAAC TACTCCTTCT ACGTCCTTGA CAACCAGAAC 
TTGCAGCAGC TGTGGGACTG GGACCACCGC AACCTGACCA TCAAAGCTGG GAAAATGTAC 
TTTGCGTTCA ATCCCAAATT GTGTGTCTCG GAGATTTACC GCATGGAGGA AGTGACGGGG 
ACTAAAGGGC GCCAGAGCAA AGGGGACATA AACACCAGGA ACAACGGGGA GAGAGCCTCC 
TGTGAAAGTG ACGTCCTGCA CTTCACCTCC ACCACCACGT CGAAGAACCG AATCATCATC 
ACCTGGCACC GGTACCGGCC CCCCGACTAC CGGGACCTCA TCAGCTTCAC CGTCTACTAC 
AAGGAAGCCC CCTTTAAAAA CGTCACGGAG TATGACGGGC AGGACGCCTG CGGCTCCAAC 
AGCTGGAACA TGGTGGACGT GGACCTCCCG CCCAACAAGG ACGTGGAGCC TGGCATTTTG 
CTGCACGGGC TGAAGCCCTG GACGCAGTAC GCCGTTTACG TGAAGGCCGT GACCCTCACC 
ATGGTGGAGA ACGACCACAT CCGTGGGGCC AAGAGCGAGA TCTTGTACAT TCGCACCAAC 
GCATCAGTTC CTTCCATCCC CCTGGATGTC CTCTCGGCCT CAAACTCCTC TTCTCAGCTG 
ATCGTGAAGT GGAACCCGCC CTCCCTGCCC AACGGCAACC TGAGCTACTA CATCGTGCGC 
TGGCAGCGCC AGCCCCAGGA CAGCTACCTG TACCGGCACA ACTACTGCTC CAAAGACAAA 
ATCCCCATCA GGAAGTACGC CGACGGCACC ATCGACGTCG AGGAGGTGAC GGAGAACCCC 
AAGACCGAGG TGTGCGGCGG GGAGAAAGGG CCGTGCTGCG CTTGTCCCAA AACCGAAGCT 
GAGAAGCAGG CGGAGAAGGA GGAGGCCGAG TACCGCAAGG TCTTCGAGAA CTTCCTGCAC 
AACGCCATCT TCGTGCCCAG ACCCGAACGG AAGCGGAGGG AGGTCATGCA GATCGCCAAC 
ACCACCATGT CCAGCCGGAG CAGGAACACC ACGGTGCTGG ACACCTACAA CATCACAGAC 
CCGGAAGAGC TCGAGACCGA GTACCCTTTC TTCGAGAGCA GAGTGGACAA CAAGGAGAGA 
ACTGTCATCT CCAACCTCCG GCCTTTTACT CTGTACCGAA TCGACATCCA CAGCTGTAAC 
CATGAGGCTG AGAAGCTTGG CTGCAGCGCC TCCAACTTTG TCTTTGCAAG AACCATGCCT 
GCAGAAGGAG CAGATGACAT TCCTGGGCCA GTGACCTGGG AGCCAAGGCC TGAGAACTCC 
ATCTTTTTAA AATGGCCAGA ACCTGAGAAT CCCAATGGAT TGATCCTCAT GTATGAAATA 
AAATATGGAT CCCAAGTCGA GGATCAGCGG GAATGTGTGT CCAGACAGGA GTACAGGAAG 
TATGGAGGAG CCAAGCTAAA CCGGCTCAAC CCAGGGAACT ACACAGCCCG GATCCAGGCC 
ACCTCTCTCT CTGGGAATGG GTCGTGGACG GATCCCGTGT TCTTCTACGT TCAGGCCAAG 
ACGACATATG AGAACTTCAT CCACCTGATG ATCGCTCTGC CCATTGCGGT TCTGTTGATA 
GTGGGAGGCT TGGTTATAAT GCTGTACGTC TTCCATAGAA AAAGAAATAG CAGCAGGCTG 
GGAAATGGAG TGCTGTATGC CTCTGTGAAC CCGGAGTATT TCAGCGCAGC TGATGTGTAC 
GTTCCTGACG AGTGGGAGGT GGCCCGGGAG AAGATCACCA TGAGCCGCGA GCTGGGCCAG 
GGCTCCTTCG GGATGGTCTA CGAAGGCGTG GCCAAGGGCG TGGTCAAGGA TGAGCCCGAG 
ACCAGGGTGG CCATTAAGAC GGTGAACGAG GCCGCCAGCA TGCGCGAGCG GATCGAGTTT 
CTCAATGAGG CCTCGGTGAT GAAGGAGTTC AATTGTCACC ACGTGGTGCG CTTGCTGGGC 
GTGGTGTCCC AGGGCCAGCC GACGCTGGTC ATCATGGAAC TGATGACCCG GGGGGACCTC 
AAGAGTTATC TCCGGTCTCT GAGGCCAGAA ATGGAGAATA ATCCAGTCCT AGCACCTCCC 
AGCCTAAGCA AAATGATCCA GATGGCTGGT GAGATTGCAG ATGGCATGGC ATACCTCAAC 
GCCAATAAGT TTGTCCACAG AGACCTCGCT GCCCGGAACT GCATGGTGGC CGAAGATTTC 
ACAGTCAAAA TCGGAGATTT TGGGATGACG AGAGACATCT ATGAGACGGA CTATTACCGG 
AAGGGGGGGA AGGGGCTGCT GCCCGTCCGC TGGATGTCCC CTGAGTCCCT CAAGGACGGA 
GTCTTCACCA CTCACTCTGA CGTCTGGTCC TTCGGGGTCG TCCTCTGGGA GATCGCCACG 
CTGGCTGAGC AGCCGTATCA GGGCCTGTCC AATGAGCAGG TCCTTCGCTT CGTCATGGAG 
GGCGGCCTTC TGGACAAGCC GGACAACTGT CCTGACATGC TGTTTGAGCT GATGCGCATG 
TGCTGGCAGT ACAACCCCAA GATGCGGCCT TCGTTCCTGG AGATCATCAG CAGCGTCAAG 
GACGAGATGG AGGCGGGCTT CCGGGAGGTC TCCTTCTACT ACAGCGAGGA GAACAAGCCG 
CCGGAGCCGG AGGAGCTGGA CCTGGAGCCC GAGAACATGG AGAGCGTGCC GCTGGACCCC 
TCGGCCTCCT CGGCCTCCCT GCCGCTGCCC GACAGACACT CAGGACACAA GGCCGAGAAC 
GGCCCGGGCC CGGGCGTGCT GGTCCTGCGG GCCAGCTTCG ACGAGAGGCA GCCCTACGCG 
CACATGAACG GGGGCCGCAA GAACGAGCGC GCCCTGCCGC TGCCCCAGTC CTCCACCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837373.1
CDS	162..3704
Translation	MLNPAHLARNAEYLLPAPAKFSTCNTVCPSACGKRACTETHECCHPECLGSCSAPDNATACVACRHYYYAGVCVPSCPPNTYRFEGWRCVDRDFCANIPNAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRQILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDSYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNAIFVPRPERKRREVMQIANTTMSSRSRNTTVLDTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTTYENFIHLMIALPIAVLLIVGGLVIMLYVFHRKRNSSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSVKDEMEAGFREVSFYYSEENKPPEPEELDLEPENMESVPLDPSASSASLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC

Target ORF information:

RefSeq Version	XM_024981605.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981605.1

ORF Insert Sequence:

ATGTTAAACC CTGCTCACCT GGCTCGAAAT GCAGAGTACT TGCTCCCTGC CCCAGCGAAA 
TTCTCAACAT GTAACACTGT GTGCCCCAGC GCGTGCGGGA AGCGGGCGTG TACGGAGACC 
CATGAGTGCT GCCACCCCGA GTGCCTGGGC AGCTGCAGCG CGCCCGACAA CGCCACGGCC 
TGCGTGGCCT GCCGCCACTA CTACTACGCC GGCGTCTGCG TGCCCAGCTG CCCGCCCAAC 
ACCTACCGCT TCGAGGGCTG GCGCTGCGTG GACCGCGACT TCTGCGCCAA CATCCCCAAC 
GCCGAGAGCA GCGACTCCGA GGGCTTCGTC ATCCACGACG GCGAGTGCAT GCAGGAGTGC 
CCGTCGGGCT TCATCCGCAA CGGGAGCCAG AGCATGTACT GCATCCCTTG CGAAGGCCCT 
TGTCCCAAAG TGTGTGAGGA AGAAAAGAAG ACAAAGACCA TTGATTCTGT CACTTCTGCT 
CAGATGCTCC AAGGATGCAC CATCTTCAAA GGCAATCTGC TCATCAACAT CCGACGCGGC 
AACAACATCG CTTCGGAACT GGAGAACTTC ATGGGGCTCA TCGAGGTGGT GACGGGCTAC 
GTGAAGATCC GCCACTCCCA CGCCTTGGTC TCCTTGTCCT TCCTGAAGAA CCTGCGCCAG 
ATCCTAGGGG AGGAGCAGCT AGAGGGGAAC TACTCCTTCT ACGTCCTTGA CAACCAGAAC 
TTGCAGCAGC TGTGGGACTG GGACCACCGC AACCTGACCA TCAAAGCTGG GAAAATGTAC 
TTTGCGTTCA ATCCCAAATT GTGTGTCTCG GAGATTTACC GCATGGAGGA AGTGACGGGG 
ACTAAAGGGC GCCAGAGCAA AGGGGACATA AACACCAGGA ACAACGGGGA GAGAGCCTCC 
TGTGAAAGTG ACGTCCTGCA CTTCACCTCC ACCACCACGT CGAAGAACCG AATCATCATC 
ACCTGGCACC GGTACCGGCC CCCCGACTAC CGGGACCTCA TCAGCTTCAC CGTCTACTAC 
AAGGAAGCCC CCTTTAAAAA CGTCACGGAG TATGACGGGC AGGACGCCTG CGGCTCCAAC 
AGCTGGAACA TGGTGGACGT GGACCTCCCG CCCAACAAGG ACGTGGAGCC TGGCATTTTG 
CTGCACGGGC TGAAGCCCTG GACGCAGTAC GCCGTTTACG TGAAGGCCGT GACCCTCACC 
ATGGTGGAGA ACGACCACAT CCGTGGGGCC AAGAGCGAGA TCTTGTACAT TCGCACCAAC 
GCATCAGTTC CTTCCATCCC CCTGGATGTC CTCTCGGCCT CAAACTCCTC TTCTCAGCTG 
ATCGTGAAGT GGAACCCGCC CTCCCTGCCC AACGGCAACC TGAGCTACTA CATCGTGCGC 
TGGCAGCGCC AGCCCCAGGA CAGCTACCTG TACCGGCACA ACTACTGCTC CAAAGACAAA 
ATCCCCATCA GGAAGTACGC CGACGGCACC ATCGACGTCG AGGAGGTGAC GGAGAACCCC 
AAGACCGAGG TGTGCGGCGG GGAGAAAGGG CCGTGCTGCG CTTGTCCCAA AACCGAAGCT 
GAGAAGCAGG CGGAGAAGGA GGAGGCCGAG TACCGCAAGG TCTTCGAGAA CTTCCTGCAC 
AACGCCATCT TCGTGCCCAG ACCCGAACGG AAGCGGAGGG AGGTCATGCA GATCGCCAAC 
ACCACCATGT CCAGCCGGAG CAGGAACACC ACGGTGCTGG ACACCTACAA CATCACAGAC 
CCGGAAGAGC TCGAGACCGA GTACCCTTTC TTCGAGAGCA GAGTGGACAA CAAGGAGAGA 
ACTGTCATCT CCAACCTCCG GCCTTTTACT CTGTACCGAA TCGACATCCA CAGCTGTAAC 
CATGAGGCTG AGAAGCTTGG CTGCAGCGCC TCCAACTTTG TCTTTGCAAG AACCATGCCT 
GCAGAAGGAG CAGATGACAT TCCTGGGCCA GTGACCTGGG AGCCAAGGCC TGAGAACTCC 
ATCTTTTTAA AATGGCCAGA ACCTGAGAAT CCCAATGGAT TGATCCTCAT GTATGAAATA 
AAATATGGAT CCCAAGTCGA GGATCAGCGG GAATGTGTGT CCAGACAGGA GTACAGGAAG 
TATGGAGGAG CCAAGCTAAA CCGGCTCAAC CCAGGGAACT ACACAGCCCG GATCCAGGCC 
ACCTCTCTCT CTGGGAATGG GTCGTGGACG GATCCCGTGT TCTTCTACGT TCAGGCCAAG 
ACGACATATG AGAACTTCAT CCACCTGATG ATCGCTCTGC CCATTGCGGT TCTGTTGATA 
GTGGGAGGCT TGGTTATAAT GCTGTACGTC TTCCATAGAA AAAGAAATAG CAGCAGGCTG 
GGAAATGGAG TGCTGTATGC CTCTGTGAAC CCGGAGTATT TCAGCGCAGC TGATGTGTAC 
GTTCCTGACG AGTGGGAGGT GGCCCGGGAG AAGATCACCA TGAGCCGCGA GCTGGGCCAG 
GGCTCCTTCG GGATGGTCTA CGAAGGCGTG GCCAAGGGCG TGGTCAAGGA TGAGCCCGAG 
ACCAGGGTGG CCATTAAGAC GGTGAACGAG GCCGCCAGCA TGCGCGAGCG GATCGAGTTT 
CTCAATGAGG CCTCGGTGAT GAAGGAGTTC AATTGTCACC ACGTGGTGCG CTTGCTGGGC 
GTGGTGTCCC AGGGCCAGCC GACGCTGGTC ATCATGGAAC TGATGACCCG GGGGGACCTC 
AAGAGTTATC TCCGGTCTCT GAGGCCAGAA ATGGAGAATA ATCCAGTCCT AGCACCTCCC 
AGCCTAAGCA AAATGATCCA GATGGCTGGT GAGATTGCAG ATGGCATGGC ATACCTCAAC 
GCCAATAAGT TTGTCCACAG AGACCTCGCT GCCCGGAACT GCATGGTGGC CGAAGATTTC 
ACAGTCAAAA TCGGAGATTT TGGGATGACG AGAGACATCT ATGAGACGGA CTATTACCGG 
AAGGGGGGGA AGGGGCTGCT GCCCGTCCGC TGGATGTCCC CTGAGTCCCT CAAGGACGGA 
GTCTTCACCA CTCACTCTGA CGTCTGGTCC TTCGGGGTCG TCCTCTGGGA GATCGCCACG 
CTGGCTGAGC AGCCGTATCA GGGCCTGTCC AATGAGCAGG TCCTTCGCTT CGTCATGGAG 
GGCGGCCTTC TGGACAAGCC GGACAACTGT CCTGACATGC TGTTTGAGCT GATGCGCATG 
TGCTGGCAGT ACAACCCCAA GATGCGGCCT TCGTTCCTGG AGATCATCAG CAGCGTCAAG 
GACGAGATGG AGGCGGGCTT CCGGGAGGTC TCCTTCTACT ACAGCGAGGA GAACAAGCCG 
CCGGAGCCGG AGGAGCTGGA CCTGGAGCCC GAGAACATGG AGAGCGTGCC GCTGGACCCC 
TCGGCCTCCT CGGCCTCCCT GCCGCTGCCC GACAGACACT CAGGACACAA GGCCGAGAAC 
GGCCCGGGCC CGGGCGTGCT GGTCCTGCGG GCCAGCTTCG ACGAGAGGCA GCCCTACGCG 
CACATGAACG GGGGCCGCAA GAACGAGCGC GCCCTGCCGC TGCCCCAGTC CTCCACCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF1R ( XM_024981606.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837374.1 Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa228212
Clone ID Related Accession (Same CDS sequence)	XM_024981606.1
Accession Version	XM_024981606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-like growth factor 1 receptor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATCT CCTCCTGGAG GTGGAATTTG TCTCTACCAC ACCGGTGGAC TTCCCAGTTT 
GGAGGTGGTG TGTGCCCCAG CGCGTGCGGG AAGCGGGCGT GTACGGAGAC CCATGAGTGC 
TGCCACCCCG AGTGCCTGGG CAGCTGCAGC GCGCCCGACA ACGCCACGGC CTGCGTGGCC 
TGCCGCCACT ACTACTACGC CGGCGTCTGC GTGCCCAGCT GCCCGCCCAA CACCTACCGC 
TTCGAGGGCT GGCGCTGCGT GGACCGCGAC TTCTGCGCCA ACATCCCCAA CGCCGAGAGC 
AGCGACTCCG AGGGCTTCGT CATCCACGAC GGCGAGTGCA TGCAGGAGTG CCCGTCGGGC 
TTCATCCGCA ACGGGAGCCA GAGCATGTAC TGCATCCCTT GCGAAGGCCC TTGTCCCAAA 
GTGTGTGAGG AAGAAAAGAA GACAAAGACC ATTGATTCTG TCACTTCTGC TCAGATGCTC 
CAAGGATGCA CCATCTTCAA AGGCAATCTG CTCATCAACA TCCGACGCGG CAACAACATC 
GCTTCGGAAC TGGAGAACTT CATGGGGCTC ATCGAGGTGG TGACGGGCTA CGTGAAGATC 
CGCCACTCCC ACGCCTTGGT CTCCTTGTCC TTCCTGAAGA ACCTGCGCCA GATCCTAGGG 
GAGGAGCAGC TAGAGGGGAA CTACTCCTTC TACGTCCTTG ACAACCAGAA CTTGCAGCAG 
CTGTGGGACT GGGACCACCG CAACCTGACC ATCAAAGCTG GGAAAATGTA CTTTGCGTTC 
AATCCCAAAT TGTGTGTCTC GGAGATTTAC CGCATGGAGG AAGTGACGGG GACTAAAGGG 
CGCCAGAGCA AAGGGGACAT AAACACCAGG AACAACGGGG AGAGAGCCTC CTGTGAAAGT 
GACGTCCTGC ACTTCACCTC CACCACCACG TCGAAGAACC GAATCATCAT CACCTGGCAC 
CGGTACCGGC CCCCCGACTA CCGGGACCTC ATCAGCTTCA CCGTCTACTA CAAGGAAGCC 
CCCTTTAAAA ACGTCACGGA GTATGACGGG CAGGACGCCT GCGGCTCCAA CAGCTGGAAC 
ATGGTGGACG TGGACCTCCC GCCCAACAAG GACGTGGAGC CTGGCATTTT GCTGCACGGG 
CTGAAGCCCT GGACGCAGTA CGCCGTTTAC GTGAAGGCCG TGACCCTCAC CATGGTGGAG 
AACGACCACA TCCGTGGGGC CAAGAGCGAG ATCTTGTACA TTCGCACCAA CGCATCAGTT 
CCTTCCATCC CCCTGGATGT CCTCTCGGCC TCAAACTCCT CTTCTCAGCT GATCGTGAAG 
TGGAACCCGC CCTCCCTGCC CAACGGCAAC CTGAGCTACT ACATCGTGCG CTGGCAGCGC 
CAGCCCCAGG ACAGCTACCT GTACCGGCAC AACTACTGCT CCAAAGACAA AATCCCCATC 
AGGAAGTACG CCGACGGCAC CATCGACGTC GAGGAGGTGA CGGAGAACCC CAAGACCGAG 
GTGTGCGGCG GGGAGAAAGG GCCGTGCTGC GCTTGTCCCA AAACCGAAGC TGAGAAGCAG 
GCGGAGAAGG AGGAGGCCGA GTACCGCAAG GTCTTCGAGA ACTTCCTGCA CAACGCCATC 
TTCGTGCCCA GACCCGAACG GAAGCGGAGG GAGGTCATGC AGATCGCCAA CACCACCATG 
TCCAGCCGGA GCAGGAACAC CACGGTGCTG GACACCTACA ACATCACAGA CCCGGAAGAG 
CTCGAGACCG AGTACCCTTT CTTCGAGAGC AGAGTGGACA ACAAGGAGAG AACTGTCATC 
TCCAACCTCC GGCCTTTTAC TCTGTACCGA ATCGACATCC ACAGCTGTAA CCATGAGGCT 
GAGAAGCTTG GCTGCAGCGC CTCCAACTTT GTCTTTGCAA GAACCATGCC TGCAGAAGGA 
GCAGATGACA TTCCTGGGCC AGTGACCTGG GAGCCAAGGC CTGAGAACTC CATCTTTTTA 
AAATGGCCAG AACCTGAGAA TCCCAATGGA TTGATCCTCA TGTATGAAAT AAAATATGGA 
TCCCAAGTCG AGGATCAGCG GGAATGTGTG TCCAGACAGG AGTACAGGAA GTATGGAGGA 
GCCAAGCTAA ACCGGCTCAA CCCAGGGAAC TACACAGCCC GGATCCAGGC CACCTCTCTC 
TCTGGGAATG GGTCGTGGAC GGATCCCGTG TTCTTCTACG TTCAGGCCAA GACGACATAT 
GAGAACTTCA TCCACCTGAT GATCGCTCTG CCCATTGCGG TTCTGTTGAT AGTGGGAGGC 
TTGGTTATAA TGCTGTACGT CTTCCATAGA AAAAGAAATA GCAGCAGGCT GGGAAATGGA 
GTGCTGTATG CCTCTGTGAA CCCGGAGTAT TTCAGCGCAG CTGATGTGTA CGTTCCTGAC 
GAGTGGGAGG TGGCCCGGGA GAAGATCACC ATGAGCCGCG AGCTGGGCCA GGGCTCCTTC 
GGGATGGTCT ACGAAGGCGT GGCCAAGGGC GTGGTCAAGG ATGAGCCCGA GACCAGGGTG 
GCCATTAAGA CGGTGAACGA GGCCGCCAGC ATGCGCGAGC GGATCGAGTT TCTCAATGAG 
GCCTCGGTGA TGAAGGAGTT CAATTGTCAC CACGTGGTGC GCTTGCTGGG CGTGGTGTCC 
CAGGGCCAGC CGACGCTGGT CATCATGGAA CTGATGACCC GGGGGGACCT CAAGAGTTAT 
CTCCGGTCTC TGAGGCCAGA AATGGAGAAT AATCCAGTCC TAGCACCTCC CAGCCTAAGC 
AAAATGATCC AGATGGCTGG TGAGATTGCA GATGGCATGG CATACCTCAA CGCCAATAAG 
TTTGTCCACA GAGACCTCGC TGCCCGGAAC TGCATGGTGG CCGAAGATTT CACAGTCAAA 
ATCGGAGATT TTGGGATGAC GAGAGACATC TATGAGACGG ACTATTACCG GAAGGGGGGG 
AAGGGGCTGC TGCCCGTCCG CTGGATGTCC CCTGAGTCCC TCAAGGACGG AGTCTTCACC 
ACTCACTCTG ACGTCTGGTC CTTCGGGGTC GTCCTCTGGG AGATCGCCAC GCTGGCTGAG 
CAGCCGTATC AGGGCCTGTC CAATGAGCAG GTCCTTCGCT TCGTCATGGA GGGCGGCCTT 
CTGGACAAGC CGGACAACTG TCCTGACATG CTGTTTGAGC TGATGCGCAT GTGCTGGCAG 
TACAACCCCA AGATGCGGCC TTCGTTCCTG GAGATCATCA GCAGCGTCAA GGACGAGATG 
GAGGCGGGCT TCCGGGAGGT CTCCTTCTAC TACAGCGAGG AGAACAAGCC GCCGGAGCCG 
GAGGAGCTGG ACCTGGAGCC CGAGAACATG GAGAGCGTGC CGCTGGACCC CTCGGCCTCC 
TCGGCCTCCC TGCCGCTGCC CGACAGACAC TCAGGACACA AGGCCGAGAA CGGCCCGGGC 
CCGGGCGTGC TGGTCCTGCG GGCCAGCTTC GACGAGAGGC AGCCCTACGC GCACATGAAC 
GGGGGCCGCA AGAACGAGCG CGCCCTGCCG CTGCCCCAGT CCTCCACCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837374.1
CDS	148..3681
Translation	MWISSWRWNLSLPHRWTSQFGGGVCPSACGKRACTETHECCHPECLGSCSAPDNATACVACRHYYYAGVCVPSCPPNTYRFEGWRCVDRDFCANIPNAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRQILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDSYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNAIFVPRPERKRREVMQIANTTMSSRSRNTTVLDTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTTYENFIHLMIALPIAVLLIVGGLVIMLYVFHRKRNSSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSVKDEMEAGFREVSFYYSEENKPPEPEELDLEPENMESVPLDPSASSASLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC

Target ORF information:

RefSeq Version	XM_024981606.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981606.1

ORF Insert Sequence:

ATGTGGATCT CCTCCTGGAG GTGGAATTTG TCTCTACCAC ACCGGTGGAC TTCCCAGTTT 
GGAGGTGGTG TGTGCCCCAG CGCGTGCGGG AAGCGGGCGT GTACGGAGAC CCATGAGTGC 
TGCCACCCCG AGTGCCTGGG CAGCTGCAGC GCGCCCGACA ACGCCACGGC CTGCGTGGCC 
TGCCGCCACT ACTACTACGC CGGCGTCTGC GTGCCCAGCT GCCCGCCCAA CACCTACCGC 
TTCGAGGGCT GGCGCTGCGT GGACCGCGAC TTCTGCGCCA ACATCCCCAA CGCCGAGAGC 
AGCGACTCCG AGGGCTTCGT CATCCACGAC GGCGAGTGCA TGCAGGAGTG CCCGTCGGGC 
TTCATCCGCA ACGGGAGCCA GAGCATGTAC TGCATCCCTT GCGAAGGCCC TTGTCCCAAA 
GTGTGTGAGG AAGAAAAGAA GACAAAGACC ATTGATTCTG TCACTTCTGC TCAGATGCTC 
CAAGGATGCA CCATCTTCAA AGGCAATCTG CTCATCAACA TCCGACGCGG CAACAACATC 
GCTTCGGAAC TGGAGAACTT CATGGGGCTC ATCGAGGTGG TGACGGGCTA CGTGAAGATC 
CGCCACTCCC ACGCCTTGGT CTCCTTGTCC TTCCTGAAGA ACCTGCGCCA GATCCTAGGG 
GAGGAGCAGC TAGAGGGGAA CTACTCCTTC TACGTCCTTG ACAACCAGAA CTTGCAGCAG 
CTGTGGGACT GGGACCACCG CAACCTGACC ATCAAAGCTG GGAAAATGTA CTTTGCGTTC 
AATCCCAAAT TGTGTGTCTC GGAGATTTAC CGCATGGAGG AAGTGACGGG GACTAAAGGG 
CGCCAGAGCA AAGGGGACAT AAACACCAGG AACAACGGGG AGAGAGCCTC CTGTGAAAGT 
GACGTCCTGC ACTTCACCTC CACCACCACG TCGAAGAACC GAATCATCAT CACCTGGCAC 
CGGTACCGGC CCCCCGACTA CCGGGACCTC ATCAGCTTCA CCGTCTACTA CAAGGAAGCC 
CCCTTTAAAA ACGTCACGGA GTATGACGGG CAGGACGCCT GCGGCTCCAA CAGCTGGAAC 
ATGGTGGACG TGGACCTCCC GCCCAACAAG GACGTGGAGC CTGGCATTTT GCTGCACGGG 
CTGAAGCCCT GGACGCAGTA CGCCGTTTAC GTGAAGGCCG TGACCCTCAC CATGGTGGAG 
AACGACCACA TCCGTGGGGC CAAGAGCGAG ATCTTGTACA TTCGCACCAA CGCATCAGTT 
CCTTCCATCC CCCTGGATGT CCTCTCGGCC TCAAACTCCT CTTCTCAGCT GATCGTGAAG 
TGGAACCCGC CCTCCCTGCC CAACGGCAAC CTGAGCTACT ACATCGTGCG CTGGCAGCGC 
CAGCCCCAGG ACAGCTACCT GTACCGGCAC AACTACTGCT CCAAAGACAA AATCCCCATC 
AGGAAGTACG CCGACGGCAC CATCGACGTC GAGGAGGTGA CGGAGAACCC CAAGACCGAG 
GTGTGCGGCG GGGAGAAAGG GCCGTGCTGC GCTTGTCCCA AAACCGAAGC TGAGAAGCAG 
GCGGAGAAGG AGGAGGCCGA GTACCGCAAG GTCTTCGAGA ACTTCCTGCA CAACGCCATC 
TTCGTGCCCA GACCCGAACG GAAGCGGAGG GAGGTCATGC AGATCGCCAA CACCACCATG 
TCCAGCCGGA GCAGGAACAC CACGGTGCTG GACACCTACA ACATCACAGA CCCGGAAGAG 
CTCGAGACCG AGTACCCTTT CTTCGAGAGC AGAGTGGACA ACAAGGAGAG AACTGTCATC 
TCCAACCTCC GGCCTTTTAC TCTGTACCGA ATCGACATCC ACAGCTGTAA CCATGAGGCT 
GAGAAGCTTG GCTGCAGCGC CTCCAACTTT GTCTTTGCAA GAACCATGCC TGCAGAAGGA 
GCAGATGACA TTCCTGGGCC AGTGACCTGG GAGCCAAGGC CTGAGAACTC CATCTTTTTA 
AAATGGCCAG AACCTGAGAA TCCCAATGGA TTGATCCTCA TGTATGAAAT AAAATATGGA 
TCCCAAGTCG AGGATCAGCG GGAATGTGTG TCCAGACAGG AGTACAGGAA GTATGGAGGA 
GCCAAGCTAA ACCGGCTCAA CCCAGGGAAC TACACAGCCC GGATCCAGGC CACCTCTCTC 
TCTGGGAATG GGTCGTGGAC GGATCCCGTG TTCTTCTACG TTCAGGCCAA GACGACATAT 
GAGAACTTCA TCCACCTGAT GATCGCTCTG CCCATTGCGG TTCTGTTGAT AGTGGGAGGC 
TTGGTTATAA TGCTGTACGT CTTCCATAGA AAAAGAAATA GCAGCAGGCT GGGAAATGGA 
GTGCTGTATG CCTCTGTGAA CCCGGAGTAT TTCAGCGCAG CTGATGTGTA CGTTCCTGAC 
GAGTGGGAGG TGGCCCGGGA GAAGATCACC ATGAGCCGCG AGCTGGGCCA GGGCTCCTTC 
GGGATGGTCT ACGAAGGCGT GGCCAAGGGC GTGGTCAAGG ATGAGCCCGA GACCAGGGTG 
GCCATTAAGA CGGTGAACGA GGCCGCCAGC ATGCGCGAGC GGATCGAGTT TCTCAATGAG 
GCCTCGGTGA TGAAGGAGTT CAATTGTCAC CACGTGGTGC GCTTGCTGGG CGTGGTGTCC 
CAGGGCCAGC CGACGCTGGT CATCATGGAA CTGATGACCC GGGGGGACCT CAAGAGTTAT 
CTCCGGTCTC TGAGGCCAGA AATGGAGAAT AATCCAGTCC TAGCACCTCC CAGCCTAAGC 
AAAATGATCC AGATGGCTGG TGAGATTGCA GATGGCATGG CATACCTCAA CGCCAATAAG 
TTTGTCCACA GAGACCTCGC TGCCCGGAAC TGCATGGTGG CCGAAGATTT CACAGTCAAA 
ATCGGAGATT TTGGGATGAC GAGAGACATC TATGAGACGG ACTATTACCG GAAGGGGGGG 
AAGGGGCTGC TGCCCGTCCG CTGGATGTCC CCTGAGTCCC TCAAGGACGG AGTCTTCACC 
ACTCACTCTG ACGTCTGGTC CTTCGGGGTC GTCCTCTGGG AGATCGCCAC GCTGGCTGAG 
CAGCCGTATC AGGGCCTGTC CAATGAGCAG GTCCTTCGCT TCGTCATGGA GGGCGGCCTT 
CTGGACAAGC CGGACAACTG TCCTGACATG CTGTTTGAGC TGATGCGCAT GTGCTGGCAG 
TACAACCCCA AGATGCGGCC TTCGTTCCTG GAGATCATCA GCAGCGTCAA GGACGAGATG 
GAGGCGGGCT TCCGGGAGGT CTCCTTCTAC TACAGCGAGG AGAACAAGCC GCCGGAGCCG 
GAGGAGCTGG ACCTGGAGCC CGAGAACATG GAGAGCGTGC CGCTGGACCC CTCGGCCTCC 
TCGGCCTCCC TGCCGCTGCC CGACAGACAC TCAGGACACA AGGCCGAGAA CGGCCCGGGC 
CCGGGCGTGC TGGTCCTGCG GGCCAGCTTC GACGAGAGGC AGCCCTACGC GCACATGAAC 
GGGGGCCGCA AGAACGAGCG CGCCCTGCCG CTGCCCCAGT CCTCCACCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF1R ( XM_024981607.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837375.1 Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa228213
Clone ID Related Accession (Same CDS sequence)	XM_024981607.1
Accession Version	XM_024981607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	insulin-like growth factor 1 receptor isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGT GCCCGTCGGG CTTCATCCGC AACGGGAGCC AGAGCATGTA CTGCATCCCT 
TGCGAAGGCC CTTGTCCCAA AGTGTGTGAG GAAGAAAAGA AGACAAAGAC CATTGATTCT 
GTCACTTCTG CTCAGATGCT CCAAGGATGC ACCATCTTCA AAGGCAATCT GCTCATCAAC 
ATCCGACGCG GCAACAACAT CGCTTCGGAA CTGGAGAACT TCATGGGGCT CATCGAGGTG 
GTGACGGGCT ACGTGAAGAT CCGCCACTCC CACGCCTTGG TCTCCTTGTC CTTCCTGAAG 
AACCTGCGCC AGATCCTAGG GGAGGAGCAG CTAGAGGGGA ACTACTCCTT CTACGTCCTT 
GACAACCAGA ACTTGCAGCA GCTGTGGGAC TGGGACCACC GCAACCTGAC CATCAAAGCT 
GGGAAAATGT ACTTTGCGTT CAATCCCAAA TTGTGTGTCT CGGAGATTTA CCGCATGGAG 
GAAGTGACGG GGACTAAAGG GCGCCAGAGC AAAGGGGACA TAAACACCAG GAACAACGGG 
GAGAGAGCCT CCTGTGAAAG TGACGTCCTG CACTTCACCT CCACCACCAC GTCGAAGAAC 
CGAATCATCA TCACCTGGCA CCGGTACCGG CCCCCCGACT ACCGGGACCT CATCAGCTTC 
ACCGTCTACT ACAAGGAAGC CCCCTTTAAA AACGTCACGG AGTATGACGG GCAGGACGCC 
TGCGGCTCCA ACAGCTGGAA CATGGTGGAC GTGGACCTCC CGCCCAACAA GGACGTGGAG 
CCTGGCATTT TGCTGCACGG GCTGAAGCCC TGGACGCAGT ACGCCGTTTA CGTGAAGGCC 
GTGACCCTCA CCATGGTGGA GAACGACCAC ATCCGTGGGG CCAAGAGCGA GATCTTGTAC 
ATTCGCACCA ACGCATCAGT TCCTTCCATC CCCCTGGATG TCCTCTCGGC CTCAAACTCC 
TCTTCTCAGC TGATCGTGAA GTGGAACCCG CCCTCCCTGC CCAACGGCAA CCTGAGCTAC 
TACATCGTGC GCTGGCAGCG CCAGCCCCAG GACAGCTACC TGTACCGGCA CAACTACTGC 
TCCAAAGACA AAATCCCCAT CAGGAAGTAC GCCGACGGCA CCATCGACGT CGAGGAGGTG 
ACGGAGAACC CCAAGACCGA GGTGTGCGGC GGGGAGAAAG GGCCGTGCTG CGCTTGTCCC 
AAAACCGAAG CTGAGAAGCA GGCGGAGAAG GAGGAGGCCG AGTACCGCAA GGTCTTCGAG 
AACTTCCTGC ACAACGCCAT CTTCGTGCCC AGACCCGAAC GGAAGCGGAG GGAGGTCATG 
CAGATCGCCA ACACCACCAT GTCCAGCCGG AGCAGGAACA CCACGGTGCT GGACACCTAC 
AACATCACAG ACCCGGAAGA GCTCGAGACC GAGTACCCTT TCTTCGAGAG CAGAGTGGAC 
AACAAGGAGA GAACTGTCAT CTCCAACCTC CGGCCTTTTA CTCTGTACCG AATCGACATC 
CACAGCTGTA ACCATGAGGC TGAGAAGCTT GGCTGCAGCG CCTCCAACTT TGTCTTTGCA 
AGAACCATGC CTGCAGAAGG AGCAGATGAC ATTCCTGGGC CAGTGACCTG GGAGCCAAGG 
CCTGAGAACT CCATCTTTTT AAAATGGCCA GAACCTGAGA ATCCCAATGG ATTGATCCTC 
ATGTATGAAA TAAAATATGG ATCCCAAGTC GAGGATCAGC GGGAATGTGT GTCCAGACAG 
GAGTACAGGA AGTATGGAGG AGCCAAGCTA AACCGGCTCA ACCCAGGGAA CTACACAGCC 
CGGATCCAGG CCACCTCTCT CTCTGGGAAT GGGTCGTGGA CGGATCCCGT GTTCTTCTAC 
GTTCAGGCCA AGACGACATA TGAGAACTTC ATCCACCTGA TGATCGCTCT GCCCATTGCG 
GTTCTGTTGA TAGTGGGAGG CTTGGTTATA ATGCTGTACG TCTTCCATAG AAAAAGAAAT 
AGCAGCAGGC TGGGAAATGG AGTGCTGTAT GCCTCTGTGA ACCCGGAGTA TTTCAGCGCA 
GCTGATGTGT ACGTTCCTGA CGAGTGGGAG GTGGCCCGGG AGAAGATCAC CATGAGCCGC 
GAGCTGGGCC AGGGCTCCTT CGGGATGGTC TACGAAGGCG TGGCCAAGGG CGTGGTCAAG 
GATGAGCCCG AGACCAGGGT GGCCATTAAG ACGGTGAACG AGGCCGCCAG CATGCGCGAG 
CGGATCGAGT TTCTCAATGA GGCCTCGGTG ATGAAGGAGT TCAATTGTCA CCACGTGGTG 
CGCTTGCTGG GCGTGGTGTC CCAGGGCCAG CCGACGCTGG TCATCATGGA ACTGATGACC 
CGGGGGGACC TCAAGAGTTA TCTCCGGTCT CTGAGGCCAG AAATGGAGAA TAATCCAGTC 
CTAGCACCTC CCAGCCTAAG CAAAATGATC CAGATGGCTG GTGAGATTGC AGATGGCATG 
GCATACCTCA ACGCCAATAA GTTTGTCCAC AGAGACCTCG CTGCCCGGAA CTGCATGGTG 
GCCGAAGATT TCACAGTCAA AATCGGAGAT TTTGGGATGA CGAGAGACAT CTATGAGACG 
GACTATTACC GGAAGGGGGG GAAGGGGCTG CTGCCCGTCC GCTGGATGTC CCCTGAGTCC 
CTCAAGGACG GAGTCTTCAC CACTCACTCT GACGTCTGGT CCTTCGGGGT CGTCCTCTGG 
GAGATCGCCA CGCTGGCTGA GCAGCCGTAT CAGGGCCTGT CCAATGAGCA GGTCCTTCGC 
TTCGTCATGG AGGGCGGCCT TCTGGACAAG CCGGACAACT GTCCTGACAT GCTGTTTGAG 
CTGATGCGCA TGTGCTGGCA GTACAACCCC AAGATGCGGC CTTCGTTCCT GGAGATCATC 
AGCAGCGTCA AGGACGAGAT GGAGGCGGGC TTCCGGGAGG TCTCCTTCTA CTACAGCGAG 
GAGAACAAGC CGCCGGAGCC GGAGGAGCTG GACCTGGAGC CCGAGAACAT GGAGAGCGTG 
CCGCTGGACC CCTCGGCCTC CTCGGCCTCC CTGCCGCTGC CCGACAGACA CTCAGGACAC 
AAGGCCGAGA ACGGCCCGGG CCCGGGCGTG CTGGTCCTGC GGGCCAGCTT CGACGAGAGG 
CAGCCCTACG CGCACATGAA CGGGGGCCGC AAGAACGAGC GCGCCCTGCC GCTGCCCCAG 
TCCTCCACCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837375.1
CDS	319..3513
Translation	MQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRQILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDSYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNAIFVPRPERKRREVMQIANTTMSSRSRNTTVLDTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTTYENFIHLMIALPIAVLLIVGGLVIMLYVFHRKRNSSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSVKDEMEAGFREVSFYYSEENKPPEPEELDLEPENMESVPLDPSASSASLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC

Target ORF information:

RefSeq Version	XM_024981607.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981607.1

ORF Insert Sequence:

ATGCAGGAGT GCCCGTCGGG CTTCATCCGC AACGGGAGCC AGAGCATGTA CTGCATCCCT 
TGCGAAGGCC CTTGTCCCAA AGTGTGTGAG GAAGAAAAGA AGACAAAGAC CATTGATTCT 
GTCACTTCTG CTCAGATGCT CCAAGGATGC ACCATCTTCA AAGGCAATCT GCTCATCAAC 
ATCCGACGCG GCAACAACAT CGCTTCGGAA CTGGAGAACT TCATGGGGCT CATCGAGGTG 
GTGACGGGCT ACGTGAAGAT CCGCCACTCC CACGCCTTGG TCTCCTTGTC CTTCCTGAAG 
AACCTGCGCC AGATCCTAGG GGAGGAGCAG CTAGAGGGGA ACTACTCCTT CTACGTCCTT 
GACAACCAGA ACTTGCAGCA GCTGTGGGAC TGGGACCACC GCAACCTGAC CATCAAAGCT 
GGGAAAATGT ACTTTGCGTT CAATCCCAAA TTGTGTGTCT CGGAGATTTA CCGCATGGAG 
GAAGTGACGG GGACTAAAGG GCGCCAGAGC AAAGGGGACA TAAACACCAG GAACAACGGG 
GAGAGAGCCT CCTGTGAAAG TGACGTCCTG CACTTCACCT CCACCACCAC GTCGAAGAAC 
CGAATCATCA TCACCTGGCA CCGGTACCGG CCCCCCGACT ACCGGGACCT CATCAGCTTC 
ACCGTCTACT ACAAGGAAGC CCCCTTTAAA AACGTCACGG AGTATGACGG GCAGGACGCC 
TGCGGCTCCA ACAGCTGGAA CATGGTGGAC GTGGACCTCC CGCCCAACAA GGACGTGGAG 
CCTGGCATTT TGCTGCACGG GCTGAAGCCC TGGACGCAGT ACGCCGTTTA CGTGAAGGCC 
GTGACCCTCA CCATGGTGGA GAACGACCAC ATCCGTGGGG CCAAGAGCGA GATCTTGTAC 
ATTCGCACCA ACGCATCAGT TCCTTCCATC CCCCTGGATG TCCTCTCGGC CTCAAACTCC 
TCTTCTCAGC TGATCGTGAA GTGGAACCCG CCCTCCCTGC CCAACGGCAA CCTGAGCTAC 
TACATCGTGC GCTGGCAGCG CCAGCCCCAG GACAGCTACC TGTACCGGCA CAACTACTGC 
TCCAAAGACA AAATCCCCAT CAGGAAGTAC GCCGACGGCA CCATCGACGT CGAGGAGGTG 
ACGGAGAACC CCAAGACCGA GGTGTGCGGC GGGGAGAAAG GGCCGTGCTG CGCTTGTCCC 
AAAACCGAAG CTGAGAAGCA GGCGGAGAAG GAGGAGGCCG AGTACCGCAA GGTCTTCGAG 
AACTTCCTGC ACAACGCCAT CTTCGTGCCC AGACCCGAAC GGAAGCGGAG GGAGGTCATG 
CAGATCGCCA ACACCACCAT GTCCAGCCGG AGCAGGAACA CCACGGTGCT GGACACCTAC 
AACATCACAG ACCCGGAAGA GCTCGAGACC GAGTACCCTT TCTTCGAGAG CAGAGTGGAC 
AACAAGGAGA GAACTGTCAT CTCCAACCTC CGGCCTTTTA CTCTGTACCG AATCGACATC 
CACAGCTGTA ACCATGAGGC TGAGAAGCTT GGCTGCAGCG CCTCCAACTT TGTCTTTGCA 
AGAACCATGC CTGCAGAAGG AGCAGATGAC ATTCCTGGGC CAGTGACCTG GGAGCCAAGG 
CCTGAGAACT CCATCTTTTT AAAATGGCCA GAACCTGAGA ATCCCAATGG ATTGATCCTC 
ATGTATGAAA TAAAATATGG ATCCCAAGTC GAGGATCAGC GGGAATGTGT GTCCAGACAG 
GAGTACAGGA AGTATGGAGG AGCCAAGCTA AACCGGCTCA ACCCAGGGAA CTACACAGCC 
CGGATCCAGG CCACCTCTCT CTCTGGGAAT GGGTCGTGGA CGGATCCCGT GTTCTTCTAC 
GTTCAGGCCA AGACGACATA TGAGAACTTC ATCCACCTGA TGATCGCTCT GCCCATTGCG 
GTTCTGTTGA TAGTGGGAGG CTTGGTTATA ATGCTGTACG TCTTCCATAG AAAAAGAAAT 
AGCAGCAGGC TGGGAAATGG AGTGCTGTAT GCCTCTGTGA ACCCGGAGTA TTTCAGCGCA 
GCTGATGTGT ACGTTCCTGA CGAGTGGGAG GTGGCCCGGG AGAAGATCAC CATGAGCCGC 
GAGCTGGGCC AGGGCTCCTT CGGGATGGTC TACGAAGGCG TGGCCAAGGG CGTGGTCAAG 
GATGAGCCCG AGACCAGGGT GGCCATTAAG ACGGTGAACG AGGCCGCCAG CATGCGCGAG 
CGGATCGAGT TTCTCAATGA GGCCTCGGTG ATGAAGGAGT TCAATTGTCA CCACGTGGTG 
CGCTTGCTGG GCGTGGTGTC CCAGGGCCAG CCGACGCTGG TCATCATGGA ACTGATGACC 
CGGGGGGACC TCAAGAGTTA TCTCCGGTCT CTGAGGCCAG AAATGGAGAA TAATCCAGTC 
CTAGCACCTC CCAGCCTAAG CAAAATGATC CAGATGGCTG GTGAGATTGC AGATGGCATG 
GCATACCTCA ACGCCAATAA GTTTGTCCAC AGAGACCTCG CTGCCCGGAA CTGCATGGTG 
GCCGAAGATT TCACAGTCAA AATCGGAGAT TTTGGGATGA CGAGAGACAT CTATGAGACG 
GACTATTACC GGAAGGGGGG GAAGGGGCTG CTGCCCGTCC GCTGGATGTC CCCTGAGTCC 
CTCAAGGACG GAGTCTTCAC CACTCACTCT GACGTCTGGT CCTTCGGGGT CGTCCTCTGG 
GAGATCGCCA CGCTGGCTGA GCAGCCGTAT CAGGGCCTGT CCAATGAGCA GGTCCTTCGC 
TTCGTCATGG AGGGCGGCCT TCTGGACAAG CCGGACAACT GTCCTGACAT GCTGTTTGAG 
CTGATGCGCA TGTGCTGGCA GTACAACCCC AAGATGCGGC CTTCGTTCCT GGAGATCATC 
AGCAGCGTCA AGGACGAGAT GGAGGCGGGC TTCCGGGAGG TCTCCTTCTA CTACAGCGAG 
GAGAACAAGC CGCCGGAGCC GGAGGAGCTG GACCTGGAGC CCGAGAACAT GGAGAGCGTG 
CCGCTGGACC CCTCGGCCTC CTCGGCCTCC CTGCCGCTGC CCGACAGACA CTCAGGACAC 
AAGGCCGAGA ACGGCCCGGG CCCGGGCGTG CTGGTCCTGC GGGCCAGCTT CGACGAGAGG 
CAGCCCTACG CGCACATGAA CGGGGGCCGC AAGAACGAGC GCGCCCTGCC GCTGCCCCAG 
TCCTCCACCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF1R ( XM_010816884.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815186.2 Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X1, mRNA.

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CloneID	OBa94164
Clone ID Related Accession (Same CDS sequence)	XM_010816884.2
Accession Version	XM_010816884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	insulin-like growth factor 1 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104530.1) and transcript sequence (NM_001244612.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 17 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGAATTT GGTCCCAAAT AAAAGGAATG AAGTCTAGTT CCAGAGGAGG GTCCCCGACC 
TCGCTGTGGG GACTCCTGTT TTTCTCCGCC GCGCTCTCGC TCTGGCCCAC GAGTGGAGAA 
ATCTGCGGGC CGGGCATCGA CATCCGCAAC GACTACCAGC AGCTGAAGCG CCTGGAGAAC 
TGCACGGTGA TCGAGGGCTA CCTCCACATC CTGCTCATTT CCAAGGCCGA GGACTACCGC 
AGCTACCGCT TCCCCAAGCT CACCGTCATC ACCGAGTACC TGCTGCTGTT CCGCGTGGCC 
GGCCTCGAGA GCCTGGGCGA CCTCTTCCCC AACCTCACGG TCATCCGCGG CTGGAAGCTC 
TTCTACAACT ACGCCCTGGT CATCTTCGAG ATGACCAACC TCAAGGACAT CGGGCTCTAC 
AACCTGCGGA ATATCACCCG TGGGGCCATC CGGATCGAGA AGAACGCCGA CCTGTGCTAC 
CTGTCCACGG TGGACTGGTC CCTGATCCTG GACGCGGCAT CCAACAACTA CATCGTGGGG 
AACAAGCCCC CCAAGGAGTG TGGGGACCTG TGCCCAGGGA CGATGGAGGA GAAGCCGCTG 
TGTGAGAAGA CGACCATCAA CAACGAGTAC AGCTACCGCT GCTGGACGAC AAACCGCTGC 
CAGAAAATGT GCCCCAGCGC GTGCGGGAAG CGGGCGTGTA CGGAGACCCA TGAGTGCTGC 
CACCCCGAGT GCCTGGGCAG CTGCAGCGCG CCCGACAACG CCACGGCCTG CGTGGCCTGC 
CGCCACTACT ACTACGCCGG CGTCTGCGTG CCCAGCTGCC CGCCCAACAC CTACCGCTTC 
GAGGGCTGGC GCTGCGTGGA CCGCGACTTC TGCGCCAACA TCCCCAACGC CGAGAGCAGC 
GACTCCGAGG GCTTCGTCAT CCACGACGGC GAGTGCATGC AGGAGTGCCC GTCGGGCTTC 
ATCCGCAACG GGAGCCAGAG CATGTACTGC ATCCCTTGCG AAGGCCCTTG TCCCAAAGTG 
TGTGAGGAAG AAAAGAAGAC AAAGACCATT GATTCTGTCA CTTCTGCTCA GATGCTCCAA 
GGATGCACCA TCTTCAAAGG CAATCTGCTC ATCAACATCC GACGCGGCAA CAACATCGCT 
TCGGAACTGG AGAACTTCAT GGGGCTCATC GAGGTGGTGA CGGGCTACGT GAAGATCCGC 
CACTCCCACG CCTTGGTCTC CTTGTCCTTC CTGAAGAACC TGCGCCAGAT CCTAGGGGAG 
GAGCAGCTAG AGGGGAACTA CTCCTTCTAC GTCCTTGACA ACCAGAACTT GCAGCAGCTG 
TGGGACTGGG ACCACCGCAA CCTGACCATC AAAGCTGGGA AAATGTACTT TGCGTTCAAT 
CCCAAATTGT GTGTCTCGGA GATTTACCGC ATGGAGGAAG TGACGGGGAC TAAAGGGCGC 
CAGAGCAAAG GGGACATAAA CACCAGGAAC AACGGGGAGA GAGCCTCCTG TGAAAGTGAT 
GTCCTGCACT TCACCTCCAC CACCACGTCG AAGAACCGAA TCATCATCAC CTGGCACCGG 
TACCGGCCCC CCGACTACCG GGACCTCATC AGCTTCACCG TCTACTACAA GGAAGCCCCC 
TTTAAAAACG TCACGGAGTA TGACGGGCAG GACGCCTGCG GCTCCAACAG CTGGAACATG 
GTGGACGTGG ACCTCCCGCC CAACAAGGAC GTGGAGCCTG GCATTTTGCT GCACGGGCTG 
AAGCCCTGGA CGCAGTACGC CGTTTACGTG AAGGCCGTGA CCCTCACCAT GGTGGAGAAC 
GACCACATCC GTGGGGCCAA GAGCGAGATC TTGTACATTC GCACCAACGC ATCAGTTCCT 
TCCATCCCCC TGGATGTCCT CTCGGCCTCA AACTCCTCTT CTCAGCTGAT CGTGAAGTGG 
AACCCGCCCT CCCTGCCCAA CGGCAACCTG AGCTACTACA TCGTGCGCTG GCAGCGCCAG 
CCCCAGGACA GCTACCTGTA CCGGCACAAC TACTGCTCCA AAGACAAAAT CCCCATCAGG 
AAGTACGCCG ACGGCACCAT CGACGTCGAG GAGGTGACGG AGAACCCCAA GACCGAGGTG 
TGCGGCGGGG AGAAAGGGCC GTGCTGCGCT TGTCCCAAAA CCGAAGCTGA GAAGCAGGCG 
GAGAAGGAGG AGGCCGAGTA CCGCAAGGTC TTCGAGAACT TCCTGCACAA CGCCATCTTC 
GTGCCCAGAC CCGAACGGAA GCGGAGGGAG GTCATGCAGA TCGCCAACAC CACCATGTCC 
AGCCGGAGCA GGAACACCAC GGTGCTGGAC ACCTACAACA TCACAGACCC GGAAGAGCTC 
GAGACCGAGT ACCCTTTCTT CGAGAGCAGA GTGGACAACA AGGAGAGAAC TGTCATCTCC 
AACCTCCGGC CTTTTACTCT GTACCGAATC GACATCCACA GCTGTAACCA TGAGGCTGAG 
AAGCTTGGCT GCAGCGCCTC CAACTTTGTC TTTGCAAGAA CCATGCCTGC AGAAGGAGCA 
GATGACATTC CTGGGCCGGT GACCTGGGAG CCAAGGCCTG AGAACTCCAT CTTTTTAAAA 
TGGCCAGAAC CTGAGAATCC CAATGGATTG ATCCTCATGT ATGAAATAAA ATATGGATCC 
CAAGTCGAGG ATCAGCGGGA ATGTGTGTCC AGACAGGAGT ACAGGAAGTA TGGAGGAGCC 
AAGCTAAACC GGCTCAACCC AGGGAACTAC ACAGCCCGGA TCCAGGCCAC CTCTCTCTCT 
GGGAATGGGT CGTGGACGGA TCCCGTGTTC TTCTACGTTC AGGCCAAGAC GACATATGAG 
AACTTCATCC ACCTGATGAT CGCTCTGCCC ATTGCGGTTC TGTTGATAGT GGGAGGCTTG 
GTTATAATGC TGTACGTCTT CCATAGAAAA AGAAATAGCA GCAGGCTGGG AAATGGAGTG 
CTGTATGCCT CTGTGAACCC GGAGTATTTC AGCGCAGCTG ATGTGTACGT TCCTGACGAG 
TGGGAGGTGG CCCGGGAGAA GATCACCATG AGCCGCGAGC TGGGCCAGGG CTCCTTCGGG 
ATGGTCTACG AAGGCGTGGC CAAGGGCGTG GTCAAGGATG AGCCCGAGAC CAGGGTGGCC 
ATTAAGACGG TGAACGAGGC CGCCAGCATG CGCGAGCGGA TCGAGTTTCT CAATGAGGCC 
TCGGTGATGA AGGAGTTCAA TTGTCACCAC GTGGTGCGCT TGCTGGGCGT GGTGTCCCAG 
GGCCAGCCGA CGCTGGTCAT CATGGAACTG ATGACCCGGG GGGACCTCAA GAGTTATCTC 
CGGTCTCTGA GGCCAGAAAT GGAGAATAAT CCAGTCCTAG CACCTCCCAG CCTAAGCAAA 
ATGATCCAGA TGGCTGGTGA GATTGCAGAT GGCATGGCAT ACCTCAACGC CAATAAGTTT 
GTCCACAGAG ACCTCGCTGC CCGGAACTGC ATGGTGGCCG AAGATTTCAC AGTCAAAATC 
GGAGATTTTG GGATGACGAG AGACATCTAT GAGACGGACT ATTACCGGAA GGGGGGGAAG 
GGGCTGCTGC CCGTCCGCTG GATGTCCCCT GAGTCCCTCA AGGACGGAGT CTTCACCACT 
CACTCTGACG TCTGGTCCTT CGGGGTCGTC CTCTGGGAGA TCGCCACGCT GGCTGAGCAG 
CCGTATCAGG GCCTGTCCAA TGAGCAGGTC CTTCGCTTCG TCATGGAGGG CGGCCTTCTG 
GACAAGCCGG ACAACTGTCC TGACATGCTG TTTGAGCTGA TGCGCATGTG CTGGCAGTAC 
AACCCCAAGA TGCGGCCTTC GTTCCTGGAG ATCATCAGCA GCGTCAAGGA CGAGATGGAG 
GCGGGCTTCC GGGAGGTCTC CTTCTACTAC AGCGAGGAGA ACAAGCCGCC GGAGCCGGAG 
GAGCTGGACC TGGAGCCCGA GAACATGGAG AGCGTGCCGC TGGACCCCTC GGCCTCCTCG 
GCCTCCCTGC CGCTGCCCGA CAGACACTCA GGACACAAGG CCGAGAACGG CCCGGGCCCG 
GGCGTGCTGG TCCTGCGGGC CAGCTTCGAC GAGAGGCAGC CCTACGCGCA CATGAACGGG 
GGCCGCAAGA ACGAGCGCGC CCTGCCGCTG CCCCAGTCCT CCACCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815186.2
CDS	23..4153
Translation	MGIWSQIKGMKSSSRGGSPTSLWGLLFFSAALSLWPTSGEICGPGIDIRNDYQQLKRLENCTVIEGYLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAASNNYIVGNKPPKECGDLCPGTMEEKPLCEKTTINNEYSYRCWTTNRCQKMCPSACGKRACTETHECCHPECLGSCSAPDNATACVACRHYYYAGVCVPSCPPNTYRFEGWRCVDRDFCANIPNAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRQILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDSYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNAIFVPRPERKRREVMQIANTTMSSRSRNTTVLDTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTTYENFIHLMIALPIAVLLIVGGLVIMLYVFHRKRNSSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSVKDEMEAGFREVSFYYSEENKPPEPEELDLEPENMESVPLDPSASSASLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC

Target ORF information:

RefSeq Version	XM_010816884.2
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor 1 receptor (IGF1R), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816884.2

ORF Insert Sequence:

ATGGGAATTT GGTCCCAAAT AAAAGGAATG AAGTCTAGTT CCAGAGGAGG GTCCCCGACC 
TCGCTGTGGG GACTCCTGTT TTTCTCCGCC GCGCTCTCGC TCTGGCCCAC GAGTGGAGAA 
ATCTGCGGGC CGGGCATCGA CATCCGCAAC GACTACCAGC AGCTGAAGCG CCTGGAGAAC 
TGCACGGTGA TCGAGGGCTA CCTCCACATC CTGCTCATTT CCAAGGCCGA GGACTACCGC 
AGCTACCGCT TCCCCAAGCT CACCGTCATC ACCGAGTACC TGCTGCTGTT CCGCGTGGCC 
GGCCTCGAGA GCCTGGGCGA CCTCTTCCCC AACCTCACGG TCATCCGCGG CTGGAAGCTC 
TTCTACAACT ACGCCCTGGT CATCTTCGAG ATGACCAACC TCAAGGACAT CGGGCTCTAC 
AACCTGCGGA ATATCACCCG TGGGGCCATC CGGATCGAGA AGAACGCCGA CCTGTGCTAC 
CTGTCCACGG TGGACTGGTC CCTGATCCTG GACGCGGCAT CCAACAACTA CATCGTGGGG 
AACAAGCCCC CCAAGGAGTG TGGGGACCTG TGCCCAGGGA CGATGGAGGA GAAGCCGCTG 
TGTGAGAAGA CGACCATCAA CAACGAGTAC AGCTACCGCT GCTGGACGAC AAACCGCTGC 
CAGAAAATGT GCCCCAGCGC GTGCGGGAAG CGGGCGTGTA CGGAGACCCA TGAGTGCTGC 
CACCCCGAGT GCCTGGGCAG CTGCAGCGCG CCCGACAACG CCACGGCCTG CGTGGCCTGC 
CGCCACTACT ACTACGCCGG CGTCTGCGTG CCCAGCTGCC CGCCCAACAC CTACCGCTTC 
GAGGGCTGGC GCTGCGTGGA CCGCGACTTC TGCGCCAACA TCCCCAACGC CGAGAGCAGC 
GACTCCGAGG GCTTCGTCAT CCACGACGGC GAGTGCATGC AGGAGTGCCC GTCGGGCTTC 
ATCCGCAACG GGAGCCAGAG CATGTACTGC ATCCCTTGCG AAGGCCCTTG TCCCAAAGTG 
TGTGAGGAAG AAAAGAAGAC AAAGACCATT GATTCTGTCA CTTCTGCTCA GATGCTCCAA 
GGATGCACCA TCTTCAAAGG CAATCTGCTC ATCAACATCC GACGCGGCAA CAACATCGCT 
TCGGAACTGG AGAACTTCAT GGGGCTCATC GAGGTGGTGA CGGGCTACGT GAAGATCCGC 
CACTCCCACG CCTTGGTCTC CTTGTCCTTC CTGAAGAACC TGCGCCAGAT CCTAGGGGAG 
GAGCAGCTAG AGGGGAACTA CTCCTTCTAC GTCCTTGACA ACCAGAACTT GCAGCAGCTG 
TGGGACTGGG ACCACCGCAA CCTGACCATC AAAGCTGGGA AAATGTACTT TGCGTTCAAT 
CCCAAATTGT GTGTCTCGGA GATTTACCGC ATGGAGGAAG TGACGGGGAC TAAAGGGCGC 
CAGAGCAAAG GGGACATAAA CACCAGGAAC AACGGGGAGA GAGCCTCCTG TGAAAGTGAT 
GTCCTGCACT TCACCTCCAC CACCACGTCG AAGAACCGAA TCATCATCAC CTGGCACCGG 
TACCGGCCCC CCGACTACCG GGACCTCATC AGCTTCACCG TCTACTACAA GGAAGCCCCC 
TTTAAAAACG TCACGGAGTA TGACGGGCAG GACGCCTGCG GCTCCAACAG CTGGAACATG 
GTGGACGTGG ACCTCCCGCC CAACAAGGAC GTGGAGCCTG GCATTTTGCT GCACGGGCTG 
AAGCCCTGGA CGCAGTACGC CGTTTACGTG AAGGCCGTGA CCCTCACCAT GGTGGAGAAC 
GACCACATCC GTGGGGCCAA GAGCGAGATC TTGTACATTC GCACCAACGC ATCAGTTCCT 
TCCATCCCCC TGGATGTCCT CTCGGCCTCA AACTCCTCTT CTCAGCTGAT CGTGAAGTGG 
AACCCGCCCT CCCTGCCCAA CGGCAACCTG AGCTACTACA TCGTGCGCTG GCAGCGCCAG 
CCCCAGGACA GCTACCTGTA CCGGCACAAC TACTGCTCCA AAGACAAAAT CCCCATCAGG 
AAGTACGCCG ACGGCACCAT CGACGTCGAG GAGGTGACGG AGAACCCCAA GACCGAGGTG 
TGCGGCGGGG AGAAAGGGCC GTGCTGCGCT TGTCCCAAAA CCGAAGCTGA GAAGCAGGCG 
GAGAAGGAGG AGGCCGAGTA CCGCAAGGTC TTCGAGAACT TCCTGCACAA CGCCATCTTC 
GTGCCCAGAC CCGAACGGAA GCGGAGGGAG GTCATGCAGA TCGCCAACAC CACCATGTCC 
AGCCGGAGCA GGAACACCAC GGTGCTGGAC ACCTACAACA TCACAGACCC GGAAGAGCTC 
GAGACCGAGT ACCCTTTCTT CGAGAGCAGA GTGGACAACA AGGAGAGAAC TGTCATCTCC 
AACCTCCGGC CTTTTACTCT GTACCGAATC GACATCCACA GCTGTAACCA TGAGGCTGAG 
AAGCTTGGCT GCAGCGCCTC CAACTTTGTC TTTGCAAGAA CCATGCCTGC AGAAGGAGCA 
GATGACATTC CTGGGCCGGT GACCTGGGAG CCAAGGCCTG AGAACTCCAT CTTTTTAAAA 
TGGCCAGAAC CTGAGAATCC CAATGGATTG ATCCTCATGT ATGAAATAAA ATATGGATCC 
CAAGTCGAGG ATCAGCGGGA ATGTGTGTCC AGACAGGAGT ACAGGAAGTA TGGAGGAGCC 
AAGCTAAACC GGCTCAACCC AGGGAACTAC ACAGCCCGGA TCCAGGCCAC CTCTCTCTCT 
GGGAATGGGT CGTGGACGGA TCCCGTGTTC TTCTACGTTC AGGCCAAGAC GACATATGAG 
AACTTCATCC ACCTGATGAT CGCTCTGCCC ATTGCGGTTC TGTTGATAGT GGGAGGCTTG 
GTTATAATGC TGTACGTCTT CCATAGAAAA AGAAATAGCA GCAGGCTGGG AAATGGAGTG 
CTGTATGCCT CTGTGAACCC GGAGTATTTC AGCGCAGCTG ATGTGTACGT TCCTGACGAG 
TGGGAGGTGG CCCGGGAGAA GATCACCATG AGCCGCGAGC TGGGCCAGGG CTCCTTCGGG 
ATGGTCTACG AAGGCGTGGC CAAGGGCGTG GTCAAGGATG AGCCCGAGAC CAGGGTGGCC 
ATTAAGACGG TGAACGAGGC CGCCAGCATG CGCGAGCGGA TCGAGTTTCT CAATGAGGCC 
TCGGTGATGA AGGAGTTCAA TTGTCACCAC GTGGTGCGCT TGCTGGGCGT GGTGTCCCAG 
GGCCAGCCGA CGCTGGTCAT CATGGAACTG ATGACCCGGG GGGACCTCAA GAGTTATCTC 
CGGTCTCTGA GGCCAGAAAT GGAGAATAAT CCAGTCCTAG CACCTCCCAG CCTAAGCAAA 
ATGATCCAGA TGGCTGGTGA GATTGCAGAT GGCATGGCAT ACCTCAACGC CAATAAGTTT 
GTCCACAGAG ACCTCGCTGC CCGGAACTGC ATGGTGGCCG AAGATTTCAC AGTCAAAATC 
GGAGATTTTG GGATGACGAG AGACATCTAT GAGACGGACT ATTACCGGAA GGGGGGGAAG 
GGGCTGCTGC CCGTCCGCTG GATGTCCCCT GAGTCCCTCA AGGACGGAGT CTTCACCACT 
CACTCTGACG TCTGGTCCTT CGGGGTCGTC CTCTGGGAGA TCGCCACGCT GGCTGAGCAG 
CCGTATCAGG GCCTGTCCAA TGAGCAGGTC CTTCGCTTCG TCATGGAGGG CGGCCTTCTG 
GACAAGCCGG ACAACTGTCC TGACATGCTG TTTGAGCTGA TGCGCATGTG CTGGCAGTAC 
AACCCCAAGA TGCGGCCTTC GTTCCTGGAG ATCATCAGCA GCGTCAAGGA CGAGATGGAG 
GCGGGCTTCC GGGAGGTCTC CTTCTACTAC AGCGAGGAGA ACAAGCCGCC GGAGCCGGAG 
GAGCTGGACC TGGAGCCCGA GAACATGGAG AGCGTGCCGC TGGACCCCTC GGCCTCCTCG 
GCCTCCCTGC CGCTGCCCGA CAGACACTCA GGACACAAGG CCGAGAACGG CCCGGGCCCG 
GGCGTGCTGG TCCTGCGGGC CAGCTTCGAC GAGAGGCAGC CCTACGCGCA CATGAACGGG 
GGCCGCAAGA ACGAGCGCGC CCTGCCGCTG CCCCAGTCCT CCACCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IGF1R ( NM_001244612.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001231541.1 Bos taurus insulin like growth factor 1 receptor (IGF1R), mRNA.

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CloneID	OBa41254
Clone ID Related Accession (Same CDS sequence)	NM_001244612.1
Accession Version	NM_001244612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	insulin-like growth factor 1 receptor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CV979948.1, NKLS02000021.1, EH174755.1, EE901083.1, CX952835.1 and X54980.1. On or before Sep 22, 2011 this sequence version replaced XM_002696504.1, XM_606794.3. Sequence Note: This RefSeq record was created from transcripts from different breeds and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5571259.35025.1, SRR5721698.1489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTCTA GCTCCGGAGG AGGGTCCCCG ACCTCGCTGT GGGGACTCCT GTTTTTCTCC 
GCCGCGCTCT CGCTCTGGCC CACGAGTGGA GAAATCTGCG GGCCGGGCAT CGACATCCGC 
AACGACTACC AGCAGCTGAA GCGCCTGGAG AACTGCACGG TGATCGAGGG CTACCTCCAC 
ATCCTGCTCA TTTCCAAGGC CGAGGACTAC CGCAGCTACC GCTTCCCCAA GCTCACCGTC 
ATCACCGAGT ACCTGCTGCT GTCCCGCGTG GCCGGCCTCG AGAGCCTGGG CGACCTCTTC 
CCCAACCTCA CGGTCATCCG CGGCTGGAAG CTCTTCTACA ACTACGCCCT GGTCATCTTC 
GAGATGACCA ACCTCAAGGA CATCGGGCTC TACAACCTGC GGAATATCAC CCGTGGGGCC 
ATCCGGATCG AGAAGAACGC CGACCTGTGC TACCTGTCCA CGGTGGACTG GTCCCTGATC 
CTGGACGCGG CATCCAACAA CTACATCGTG GGGAACAAGC CCCCCAAGGA GTGTGGGGAC 
CTGTGCCCAG GGACGATGGA GGAGAAGCCG CTGTGTGAGA AGACGACCAT CAACAACGAG 
TACAGCTACC GCTGCTGGAC GACAAACCGC TGCCAGAAAA TGTGCCCCAG CGCGTGCGGG 
AAGCGGGCGT GTACGGAGAC CCATGAGTGC TGCCACCCCG AGTGCCTGGG CAGCTGCAGC 
GCGCCCGACA ACGCCACGGC CTGCGTGGCC TGCCGCCACT ACTACTACGC CGGCGTCTGC 
GTGCCCAGCT GCCCGCCCAA CACCTACCGC TTCGAGGGCT GGCGCTGCGT GGACCGCGAC 
TTCTGCGCCA ACATCCCCAA CGCCGAGAGC AGCGACTCCG AGGGCTTCGT CATCCACGAC 
GGCGAGTGCA TGCAGGAGTG CCCGTCGGGC TTCATCCGCA ACGGGAGCCA GAGCATGTAC 
TGCATCCCTT GCGAAGGCCC TTGTCCCAAA GTGTGTGAGG AAGAAAAGAA GACAAAGACC 
ATTGATTCTG TCACTTCTGC TCAGATGCTC CAAGGATGCA CCATCTTCAA AGGCAATCTG 
CTCATCAACA TCCGACGCGG CAACAACATC GCTTCGGAAC TGGAGAACTT CATGGGGCTC 
ATCGAGGTGG TGACGGGCTA CGTGAAGATC CGCCACTCCC ACGCCTTGGT CTCCTTGTCC 
TTCCTGAAGA ACCTGCGCCA GATCCTAGGG GAGGAGCAGC TAGAGGGGAA CTACTCCTTC 
TACGTCCTTG ACAACCAGAA CTTGCAGCAG CTGTGGGACT GGGACCACCG CAACCTGACC 
ATCAAAGCTG GGAAAATGTA CTTTGCGTTC AATCCCAAAT TGTGTGTCTC GGAGATTTAC 
CGCATGGAGG AAGTGACGGG GACTAAAGGG CGCCAGAGCA AAGGGGACAT AAACACCAGG 
AACAACGGGG AGAGAGCCTC CTGTGAAAGT GACGTCCTGC ACTTCACCTC CACCACCACG 
TCGAAGAACC GAATCATCAT CACCTGGCAC CGGTACCGGC CCCCCGACTA CCGGGACCTC 
ATCAGCTTCA CCGTCTACTA CAAGGAAGCC CCCTTTAAAA ACGTCACGGA GTATGACGGG 
CAGGACGCCT GCGGCTCCAA CAGCTGGAAC ATGGTGGACG TGGACCTCCC GCCCAACAAG 
GACGTGGAGC CTGGCATTTT GCTGCACGGG CTGAAGCCCT GGACGCAGTA CGCCGTTTAC 
GTGAAGGCCG TGACCCTCAC CATGGTGGAG AACGACCACA TCCGTGGGGC CAAGAGCGAG 
ATCTTGTACA TTCGCACCAA CGCATCAGTT CCTTCCATCC CCCTGGATGT CCTCTCGGCC 
TCAAACTCCT CTTCTCAGCT GATCGTGAAG TGGAACCCGC CCTCCCTGCC CAACGGCAAC 
CTGAGCTACT ACATCGTGCG CTGGCAGCGC CAGCCCCAGG ACAGCTACCT GTACCGGCAC 
AACTACTGCT CCAAAGACAA AATCCCCATC AGGAAGTACG CCGACGGCAC CATCGACGTC 
GAGGAGGTGA CGGAGAACCC CAAGACCGAG GTGTGCGGCG GGGAGAAAGG GCCGTGCTGC 
GCTTGTCCCA AAACCGAAGC TGAGAAGCAG GCGGAGAAGG AGGAGGCCGA GTACCGCAAG 
GTCTTCGAGA ACTTCCTGCA CAACGCCATC TTCGTGCCCA GACCCGAACG GAAGCGGAGG 
GAGGTCATGC AGATCGCCAA CACCACCATG TCCAGCCGGA GCAGGAACAC CACGGTGCTG 
GACACCTACA ACATCACAGA CCCGGAAGAG CTCGAGACCG AGTACCCTTT CTTCGAGAGC 
AGAGTGGACA ACAAGGAGAG AACTGTCATC TCCAACCTCC GGCCTTTTAC TCTGTACCGA 
ATCGACATCC ACAGCTGTAA CCATGAGGCT GAGAAGCTTG GCTGCAGCGC CTCCAACTTT 
GTCTTTGCAA GAACCATGCC TGCAGAAGGA GCAGATGACA TTCCTGGGCC AGTGACCTGG 
GAGCCAAGGC CTGAGAACTC CATCTTTTTA AAATGGCCAG AACCTGAGAA TCCCAATGGA 
TTGATCCTCA TGTATGAAAT AAAATATGGA TCCCAAGTCG AGGATCAGCG GGAATGTGTG 
TCCAGACAGG AGTACAGGAA GTATGGAGGA GCCAAGCTAA ACCGGCTCAA CCCAGGGAAC 
TACACAGCCC GGATCCAGGC CACCTCTCTC TCTGGGAATG GGTCGTGGAC GGATCCCGTG 
TTCTTCTACG TTCAGGCCAA GACGACATAT GAGAACTTCA TCCACCTGAT GATCGCTCTG 
CCCATTGCGG TTCTGTTGAT AGTGGGAGGC TTGGTTATAA TGCTGTACGT CTTCCATAGA 
AAAAGAAATA GCAGCAGGCT GGGAAATGGA GTGCTGTATG CCTCTGTGAA CCCGGAGTAT 
TTCAGCGCAG CTGATGTGTA CGTTCCTGAC GAGTGGGAGG TGGCCCGGGA GAAGATCACC 
ATGAGCCGCG AGCTGGGCCA GGGCTCCTTC GGGATGGTCT ACGAAGGCGT GGCCAAGGGC 
GTGGTCAAGG ATGAGCCCGA GACCAGGGTG GCCATTAAGA CGGTGAACGA GGCCGCCAGC 
ATGCGCGAGC GGATCGAGTT TCTCAATGAG GCCTCGGTGA TGAAGGAGTT CAATTGTCAC 
CACGTGGTGC GCTTGCTGGG CGTGGTGTCC CAGGGCCAGC CGACGCTGGT CATCATGGAA 
CTGATGACCC GGGGGGACCT CAAGAGTTAT CTCCGGTCTC TGAGGCCAGA AATGGAGAAT 
AATCCAGTCC TAGCACCTCC CAGCCTAAGC AAAATGATCC AGATGGCTGG TGAGATTGCA 
GATGGCATGG CATACCTCAA CGCCAATAAG TTTGTCCACA GAGACCTCGC TGCCCGGAAC 
TGCATGGTGG CCGAAGATTT CACAGTCAAA ATCGGAGATT TTGGGATGAC GAGAGACATC 
TATGAGACGG ACTATTACCG GAAGGGGGGG AAGGGGCTGC TGCCCGTCCG CTGGATGTCC 
CCTGAGTCCC TCAAGGACGG AGTCTTCACC ACTCACTCTG ACGTCTGGTC CTTCGGGGTC 
GTCCTCTGGG AGATCGCCAC GCTGGCTGAG CAGCCGTATC AGGGCCTGTC CAATGAGCAG 
GTCCTTCGCT TCGTCATGGA GGGCGGCCTT CTGGACAAGC CGGACAACTG TCCTGACATG 
CTGTTTGAGC TGATGCGCAT GTGCTGGCAG TACAACCCCA AGATGCGGCC TTCGTTCCTG 
GAGATCATCA GCAGCGTCAA GGACGAGATG GAGGCGGGCT TCCGGGAGGT CTCCTTCTAC 
TACAGCGAGG AGAACAAGCC GCCGGAGCCG GAGGAGCTGG ACCTGGAGCC CGAGAACATG 
GAGAGCGTGC CGCTGGACCC CTCGGCCTCC TCGGCCTCCC TGCCGCTGCC CGACAGACAC 
TCAGGACACA AGGCCGAGAA CGGCCCGGGC CCGGGCGTGC TGGTCCTGCG GGCCAGCTTC 
GACGAGAGGC AGCCCTACGC GCACATGAAC GGGGGCCGCA AGAACGAGCG CGCCCTGCCG 
CTGCCCCAGT CCTCCACCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231541.1
CDS	33..4136
Translation	MKSSSGGGSPTSLWGLLFFSAALSLWPTSGEICGPGIDIRNDYQQLKRLENCTVIEGYLHILLISKAEDYRSYRFPKLTVITEYLLLSRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAASNNYIVGNKPPKECGDLCPGTMEEKPLCEKTTINNEYSYRCWTTNRCQKMCPSACGKRACTETHECCHPECLGSCSAPDNATACVACRHYYYAGVCVPSCPPNTYRFEGWRCVDRDFCANIPNAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRQILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDSYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNAIFVPRPERKRREVMQIANTTMSSRSRNTTVLDTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTTYENFIHLMIALPIAVLLIVGGLVIMLYVFHRKRNSSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSVKDEMEAGFREVSFYYSEENKPPEPEELDLEPENMESVPLDPSASSASLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC

Target ORF information:

RefSeq Version	NM_001244612.1
Organism	Bos taurus(cattle)
Definition	Bos taurus insulin like growth factor 1 receptor (IGF1R), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244612.1

ORF Insert Sequence:

ATGAAGTCTA GCTCCGGAGG AGGGTCCCCG ACCTCGCTGT GGGGACTCCT GTTTTTCTCC 
GCCGCGCTCT CGCTCTGGCC CACGAGTGGA GAAATCTGCG GGCCGGGCAT CGACATCCGC 
AACGACTACC AGCAGCTGAA GCGCCTGGAG AACTGCACGG TGATCGAGGG CTACCTCCAC 
ATCCTGCTCA TTTCCAAGGC CGAGGACTAC CGCAGCTACC GCTTCCCCAA GCTCACCGTC 
ATCACCGAGT ACCTGCTGCT GTCCCGCGTG GCCGGCCTCG AGAGCCTGGG CGACCTCTTC 
CCCAACCTCA CGGTCATCCG CGGCTGGAAG CTCTTCTACA ACTACGCCCT GGTCATCTTC 
GAGATGACCA ACCTCAAGGA CATCGGGCTC TACAACCTGC GGAATATCAC CCGTGGGGCC 
ATCCGGATCG AGAAGAACGC CGACCTGTGC TACCTGTCCA CGGTGGACTG GTCCCTGATC 
CTGGACGCGG CATCCAACAA CTACATCGTG GGGAACAAGC CCCCCAAGGA GTGTGGGGAC 
CTGTGCCCAG GGACGATGGA GGAGAAGCCG CTGTGTGAGA AGACGACCAT CAACAACGAG 
TACAGCTACC GCTGCTGGAC GACAAACCGC TGCCAGAAAA TGTGCCCCAG CGCGTGCGGG 
AAGCGGGCGT GTACGGAGAC CCATGAGTGC TGCCACCCCG AGTGCCTGGG CAGCTGCAGC 
GCGCCCGACA ACGCCACGGC CTGCGTGGCC TGCCGCCACT ACTACTACGC CGGCGTCTGC 
GTGCCCAGCT GCCCGCCCAA CACCTACCGC TTCGAGGGCT GGCGCTGCGT GGACCGCGAC 
TTCTGCGCCA ACATCCCCAA CGCCGAGAGC AGCGACTCCG AGGGCTTCGT CATCCACGAC 
GGCGAGTGCA TGCAGGAGTG CCCGTCGGGC TTCATCCGCA ACGGGAGCCA GAGCATGTAC 
TGCATCCCTT GCGAAGGCCC TTGTCCCAAA GTGTGTGAGG AAGAAAAGAA GACAAAGACC 
ATTGATTCTG TCACTTCTGC TCAGATGCTC CAAGGATGCA CCATCTTCAA AGGCAATCTG 
CTCATCAACA TCCGACGCGG CAACAACATC GCTTCGGAAC TGGAGAACTT CATGGGGCTC 
ATCGAGGTGG TGACGGGCTA CGTGAAGATC CGCCACTCCC ACGCCTTGGT CTCCTTGTCC 
TTCCTGAAGA ACCTGCGCCA GATCCTAGGG GAGGAGCAGC TAGAGGGGAA CTACTCCTTC 
TACGTCCTTG ACAACCAGAA CTTGCAGCAG CTGTGGGACT GGGACCACCG CAACCTGACC 
ATCAAAGCTG GGAAAATGTA CTTTGCGTTC AATCCCAAAT TGTGTGTCTC GGAGATTTAC 
CGCATGGAGG AAGTGACGGG GACTAAAGGG CGCCAGAGCA AAGGGGACAT AAACACCAGG 
AACAACGGGG AGAGAGCCTC CTGTGAAAGT GACGTCCTGC ACTTCACCTC CACCACCACG 
TCGAAGAACC GAATCATCAT CACCTGGCAC CGGTACCGGC CCCCCGACTA CCGGGACCTC 
ATCAGCTTCA CCGTCTACTA CAAGGAAGCC CCCTTTAAAA ACGTCACGGA GTATGACGGG 
CAGGACGCCT GCGGCTCCAA CAGCTGGAAC ATGGTGGACG TGGACCTCCC GCCCAACAAG 
GACGTGGAGC CTGGCATTTT GCTGCACGGG CTGAAGCCCT GGACGCAGTA CGCCGTTTAC 
GTGAAGGCCG TGACCCTCAC CATGGTGGAG AACGACCACA TCCGTGGGGC CAAGAGCGAG 
ATCTTGTACA TTCGCACCAA CGCATCAGTT CCTTCCATCC CCCTGGATGT CCTCTCGGCC 
TCAAACTCCT CTTCTCAGCT GATCGTGAAG TGGAACCCGC CCTCCCTGCC CAACGGCAAC 
CTGAGCTACT ACATCGTGCG CTGGCAGCGC CAGCCCCAGG ACAGCTACCT GTACCGGCAC 
AACTACTGCT CCAAAGACAA AATCCCCATC AGGAAGTACG CCGACGGCAC CATCGACGTC 
GAGGAGGTGA CGGAGAACCC CAAGACCGAG GTGTGCGGCG GGGAGAAAGG GCCGTGCTGC 
GCTTGTCCCA AAACCGAAGC TGAGAAGCAG GCGGAGAAGG AGGAGGCCGA GTACCGCAAG 
GTCTTCGAGA ACTTCCTGCA CAACGCCATC TTCGTGCCCA GACCCGAACG GAAGCGGAGG 
GAGGTCATGC AGATCGCCAA CACCACCATG TCCAGCCGGA GCAGGAACAC CACGGTGCTG 
GACACCTACA ACATCACAGA CCCGGAAGAG CTCGAGACCG AGTACCCTTT CTTCGAGAGC 
AGAGTGGACA ACAAGGAGAG AACTGTCATC TCCAACCTCC GGCCTTTTAC TCTGTACCGA 
ATCGACATCC ACAGCTGTAA CCATGAGGCT GAGAAGCTTG GCTGCAGCGC CTCCAACTTT 
GTCTTTGCAA GAACCATGCC TGCAGAAGGA GCAGATGACA TTCCTGGGCC AGTGACCTGG 
GAGCCAAGGC CTGAGAACTC CATCTTTTTA AAATGGCCAG AACCTGAGAA TCCCAATGGA 
TTGATCCTCA TGTATGAAAT AAAATATGGA TCCCAAGTCG AGGATCAGCG GGAATGTGTG 
TCCAGACAGG AGTACAGGAA GTATGGAGGA GCCAAGCTAA ACCGGCTCAA CCCAGGGAAC 
TACACAGCCC GGATCCAGGC CACCTCTCTC TCTGGGAATG GGTCGTGGAC GGATCCCGTG 
TTCTTCTACG TTCAGGCCAA GACGACATAT GAGAACTTCA TCCACCTGAT GATCGCTCTG 
CCCATTGCGG TTCTGTTGAT AGTGGGAGGC TTGGTTATAA TGCTGTACGT CTTCCATAGA 
AAAAGAAATA GCAGCAGGCT GGGAAATGGA GTGCTGTATG CCTCTGTGAA CCCGGAGTAT 
TTCAGCGCAG CTGATGTGTA CGTTCCTGAC GAGTGGGAGG TGGCCCGGGA GAAGATCACC 
ATGAGCCGCG AGCTGGGCCA GGGCTCCTTC GGGATGGTCT ACGAAGGCGT GGCCAAGGGC 
GTGGTCAAGG ATGAGCCCGA GACCAGGGTG GCCATTAAGA CGGTGAACGA GGCCGCCAGC 
ATGCGCGAGC GGATCGAGTT TCTCAATGAG GCCTCGGTGA TGAAGGAGTT CAATTGTCAC 
CACGTGGTGC GCTTGCTGGG CGTGGTGTCC CAGGGCCAGC CGACGCTGGT CATCATGGAA 
CTGATGACCC GGGGGGACCT CAAGAGTTAT CTCCGGTCTC TGAGGCCAGA AATGGAGAAT 
AATCCAGTCC TAGCACCTCC CAGCCTAAGC AAAATGATCC AGATGGCTGG TGAGATTGCA 
GATGGCATGG CATACCTCAA CGCCAATAAG TTTGTCCACA GAGACCTCGC TGCCCGGAAC 
TGCATGGTGG CCGAAGATTT CACAGTCAAA ATCGGAGATT TTGGGATGAC GAGAGACATC 
TATGAGACGG ACTATTACCG GAAGGGGGGG AAGGGGCTGC TGCCCGTCCG CTGGATGTCC 
CCTGAGTCCC TCAAGGACGG AGTCTTCACC ACTCACTCTG ACGTCTGGTC CTTCGGGGTC 
GTCCTCTGGG AGATCGCCAC GCTGGCTGAG CAGCCGTATC AGGGCCTGTC CAATGAGCAG 
GTCCTTCGCT TCGTCATGGA GGGCGGCCTT CTGGACAAGC CGGACAACTG TCCTGACATG 
CTGTTTGAGC TGATGCGCAT GTGCTGGCAG TACAACCCCA AGATGCGGCC TTCGTTCCTG 
GAGATCATCA GCAGCGTCAA GGACGAGATG GAGGCGGGCT TCCGGGAGGT CTCCTTCTAC 
TACAGCGAGG AGAACAAGCC GCCGGAGCCG GAGGAGCTGG ACCTGGAGCC CGAGAACATG 
GAGAGCGTGC CGCTGGACCC CTCGGCCTCC TCGGCCTCCC TGCCGCTGCC CGACAGACAC 
TCAGGACACA AGGCCGAGAA CGGCCCGGGC CCGGGCGTGC TGGTCCTGCG GGCCAGCTTC 
GACGAGAGGC AGCCCTACGC GCACATGAAC GGGGGCCGCA AGAACGAGCG CGCCCTGCCG 
CTGCCCCAGT CCTCCACCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymorphism in exon 2 encoding the putative ligand binding pocket of the bovine insulin-like growth factor 1 receptor affects milk traits in four different cattle breeds.
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie134(1)34-42(2017 Feb)
Szewczuk M

Periconceptional undernutrition modifies endocrine profiles and hepatic gene expression in sheep.
Journal of animal physiology and animal nutrition99(4)710-8(2015 Aug)
de Brun V,Meikle A,Casal A,Sequeira M,Contreras-Sol?s I,Carriquiry M,Forcada F,Sosa C,Abecia JA

Involvement of PAPP-A and IGFR1 in Cystic Ovarian Disease in Cattle.
Reproduction in domestic animals = Zuchthygiene50(4)659-68(2015 Aug)
Rodr?guez FM,Colombero M,Amweg AN,Huber E,Gareis NC,Salvetti NR,Ortega HH,Rey F

Effects in cattle of genetic variation within the IGF1R gene on the superovulation performance and pregnancy rates after embryo transfer.
Animal reproduction science143(1-4)24-9(2013 Dec)
Yang WC,Yang LG,Riaz H,Tang KQ,Chen L,Li SJ

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IGF1R cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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