YWHAE cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	YWHAE
Entrez Gene ID	282125
Full Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
General protein information	Preferred Names tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon Names 14-3-3 protein epsilon 14-3-3E tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005220066.2	XP_005220123.1	14-3-3 protein epsilon isoform X1
NM_174491.3	NP_776916.1	14-3-3 protein epsilon
XM_024979616.1	XP_024835384.1	14-3-3 protein epsilon isoform X1
XM_005220066.3	XP_005220123.1	14-3-3 protein epsilon isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	YWHAE	NP_001233297.1
Saccharomyces cerevisiae (baker's yeast)	BMH1	NP_011104.3
Neurospora crassa	NCU02806	XP_964462.1
Arabidopsis thaliana (thale cress)	GRF3	NP_568557.1
Oryza sativa (rice)	Os04g0462500	NP_001053003.1
Oryza sativa (rice)	Os08g0430500	NP_001061856.1
Mus musculus (house mouse)	Ywhae	NP_033562.3
Arabidopsis thaliana (thale cress)	GRF5	NP_568325.1
Xenopus tropicalis (tropical clawed frog)	ywhae	NP_001008156.1
Homo sapiens (human)	YWHAE	NP_006752.1
Bos taurus (cattle)	YWHAE	NP_776916.1
Drosophila melanogaster (fruit fly)	14-3-3epsilon	NP_732311.1
Schizosaccharomyces pombe (fission yeast)	rad25	NP_594247.1
Danio rerio (zebrafish)	ywhae2	NP_001013359.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001151	XP_322009.2
Saccharomyces cerevisiae (baker's yeast)	BMH2	NP_010384.3
Eremothecium gossypii	AGOS_AGR107C	NP_986773.1
Arabidopsis thaliana (thale cress)	GRF7	NP_566174.1
Oryza sativa (rice)	Os02g0580300	NP_001047234.1
Canis lupus familiaris (dog)	YWHAE	XP_854358.1
Rattus norvegicus (Norway rat)	Ywhae	NP_113791.1
Gallus gallus (chicken)	YWHAE	NP_001006219.1
Kluyveromyces lactis	KLLA0F12100g	XP_455629.1
Magnaporthe oryzae (rice blast fungus)	MGG_01588	XP_003714567.1
Oryza sativa (rice)	Os03g0710800	NP_001051051.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Modulation of the G protein regulator phosducin by Ca2+/calmodulin-dependent protein kinase II phosphorylation and 14-3-3 protein binding.
Thulin CD, Savage JR, McLaughlin JN, Truscott SM, Old WM, Ahn NG, Resing KA, Hamm HE, Bitensky MW, Willardson BM
The Journal of biological chemistry276(26)23805-15(2001 Jun)

Rethinking the role of phosducin: light-regulated binding of phosducin to 14-3-3 in rod inner segments.
Nakano K, Chen J, Tarr GE, Yoshida T, Flynn JM, Bitensky MW
Proceedings of the National Academy of Sciences of the United States of America98(8)4693-8(2001 Apr)

Comparative analysis of 82 expressed sequence tags from a cattle ovary cDNA library.
Ma RZ, van Eijk MJ, Beever JE, Gu?rin G, Mummery CL, Lewin HA
Mammalian genome : official journal of the International Mammalian Genome Society9(7)545-9(1998 Jul)

Activation of protein kinase C by purified bovine brain 14-3-3: comparison with tyrosine hydroxylase activation.
Tanji M, Horwitz R, Rosenfeld G, Waymire JC
Journal of neurochemistry63(5)1908-16(1994 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YWHAE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YWHAE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43162	NM_174491.3 Latest version!	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa29505	XM_005220066.3 Latest version!	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa225977	XM_024979616.1 Latest version!	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OBa29505	XM_005220066.2	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YWHAE ( NM_174491.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776916.1 Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), mRNA.

Price & Availability↑

CloneID	OBa43162
Clone ID Related Accession (Same CDS sequence)	NM_174491.3
Accession Version	NM_174491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	14-3-3 protein epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102928.1. On Aug 28, 2005 this sequence version replaced NM_174491.2. ##Evidence-Data-START## Transcript exon combination :: BC102928.1, AF043735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGGTGAA 
GAGCAGAATA AAGAAGCGCT GCAGGATGTG GAAGATGAAA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776916.1
CDS	87..854
Translation	MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ

Target ORF information:

RefSeq Version	NM_174491.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174491.3

ORF Insert Sequence:

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGGTGAA 
GAGCAGAATA AAGAAGCGCT GCAGGATGTG GAAGATGAAA ATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

YWHAE ( XM_005220066.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220123.1 Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29505
Clone ID Related Accession (Same CDS sequence)	XM_005220066.2 , XM_005220066.3
Accession Version	XM_005220066.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	14-3-3 protein epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGATTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220123.1
CDS	169..891
Translation	MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDDS

Target ORF information:

RefSeq Version	XM_005220066.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220066.2

ORF Insert Sequence:

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGATTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

YWHAE ( XM_005220066.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220123.1 Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa29505
Clone ID Related Accession (Same CDS sequence)	XM_005220066.2 , XM_005220066.3
Accession Version	XM_005220066.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	14-3-3 protein epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGATTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220123.1
CDS	169..891
Translation	MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDDS

Target ORF information:

RefSeq Version	XM_005220066.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220066.3

ORF Insert Sequence:

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGATTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

YWHAE ( XM_024979616.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835384.1 Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa225977
Clone ID Related Accession (Same CDS sequence)	XM_024979616.1
Accession Version	XM_024979616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	14-3-3 protein epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGGTAAG 
GAGACTGAAG TTAAAATATG TCTCTTTCAG TTGTTGGATC TATTTACTCT TAGAAAATTG 
GGATTTCTTC ATATTATTAT GGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835384.1
CDS	169..975
Translation	MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGKETEVKICLFQLLDLFTLRKLGFLHIIMV

Target ORF information:

RefSeq Version	XM_024979616.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979616.1

ORF Insert Sequence:

ATGGATGATC GGGAGGATCT GGTGTACCAG GCGAAGCTGG CCGAGCAGGC TGAGCGATAC 
GACGAAATGG TGGAGTCAAT GAAGAAAGTA GCAGGGATGG ATGTGGAGTT GACAGTTGAA 
GAAAGAAACC TCCTATCTGT TGCATATAAA AATGTGATTG GAGCTAGAAG AGCTTCCTGG 
AGAATAATCA GCAGCATTGA ACAGAAAGAA GAAAACAAGG GAGGAGAAGA CAAACTAAAA 
ATGATTCGGG AATATCGGCA AATGGTTGAG ACTGAGCTAA AGTTAATCTG TTGTGACATT 
CTGGATGTAC TGGACAAACA CCTCATTCCA GCAGCTAACA CTGGCGAGTC CAAGGTTTTC 
TATTATAAAA TGAAAGGGGA CTACCACAGG TATCTGGCTG AATTTGCCAC AGGGAATGAC 
AGGAAGGAGG CTGCGGAGAA CAGCCTAGTG GCTTATAAAG CTGCTAGTGA TATTGCAATG 
ACAGAACTTC CACCAACACA TCCCATTCGC TTAGGTCTTG CTCTCAACTT TTCTGTATTC 
TACTATGAAA TTCTTAATTC CCCTGACCGT GCCTGCAGGT TGGCAAAAGC AGCTTTTGAT 
GATGCAATTG CAGAACTGGA CACGCTGAGT GAAGAAAGCT ATAAGGACTC TACACTTATC 
ATGCAGTTGT TACGTGATAA TCTGACACTA TGGACTTCAG ACATGCAGGG TGATGGTAAG 
GAGACTGAAG TTAAAATATG TCTCTTTCAG TTGTTGGATC TATTTACTCT TAGAAAATTG 
GGATTTCTTC ATATTATTAT GGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Modulation of the G protein regulator phosducin by Ca2+/calmodulin-dependent protein kinase II phosphorylation and 14-3-3 protein binding.
The Journal of biological chemistry276(26)23805-15(2001 Jun)
Thulin CD,Savage JR,McLaughlin JN,Truscott SM,Old WM,Ahn NG,Resing KA,Hamm HE,Bitensky MW,Willardson BM

Rethinking the role of phosducin: light-regulated binding of phosducin to 14-3-3 in rod inner segments.
Proceedings of the National Academy of Sciences of the United States of America98(8)4693-8(2001 Apr)
Nakano K,Chen J,Tarr GE,Yoshida T,Flynn JM,Bitensky MW

Comparative analysis of 82 expressed sequence tags from a cattle ovary cDNA library.
Mammalian genome : official journal of the International Mammalian Genome Society9(7)545-9(1998 Jul)
Ma RZ,van Eijk MJ,Beever JE,Gu?rin G,Mummery CL,Lewin HA

Activation of protein kinase C by purified bovine brain 14-3-3: comparison with tyrosine hydroxylase activation.
Journal of neurochemistry63(5)1908-16(1994 Nov)
Tanji M,Horwitz R,Rosenfeld G,Waymire JC

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YWHAE cDNA ORF clone, Bos taurus(cattle)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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