MMP9 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	MMP9
Entrez Gene ID	282871
Full Name	matrix metallopeptidase 9
General protein information	Preferred Names matrix metallopeptidase 9 Names matrix metalloproteinase-9 92 kDa gelatinase 92 kDa type IV collagenase GELB MMP-9 gelatinase B matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) matrix metalloproteinase 9 (gelatinase B, 92kDa matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA Protein Name

NM_174744.2 NP_777169.1 matrix metalloproteinase-9 precursor
Pathways from BioSystems

mRNA	Protein	Name
NM_174744.2	NP_777169.1	matrix metalloproteinase-9 precursor

Homologies

Bos taurus (cattle)	MMP9	NP_777169.1
Rattus norvegicus (Norway rat)	Mmp9	NP_112317.1
Danio rerio (zebrafish)	mmp9	NP_998288.1
Xenopus tropicalis (tropical clawed frog)	LOC100494738	XP_002932554.1
Pan troglodytes (chimpanzee)	MMP9	XP_003317021.1
Mus musculus (house mouse)	Mmp9	NP_038627.1
	MMP9	XP_001104871.1
Gallus gallus (chicken)	MMP9	NP_989998.1
Xenopus tropicalis (tropical clawed frog)	mmp9	NP_001006843.1
Homo sapiens (human)	MMP9	NP_004985.2
Canis lupus familiaris (dog)	MMP9	NP_001003219.1
Arabidopsis thaliana (thale cress)	AT2G45040	NP_182030.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular Mechanism of Transcriptional Regulation of Matrix Metalloproteinase-9 in Diabetic Retinopathy.
Mishra M, Flaga J, Kowluru RA
Journal of cellular physiology231(8)1709-18(2016 Aug)

Characterization of the contributions of Hp-MMP 9 to the serum acute phase protein response of lipopolysaccharide challenged calves.
Hinds CA, Niehaus AJ, Premanandan C, Rajala-Schultz PJ, Rings DM, Lakritz J
BMC veterinary research10261(2014 Oct)

Interrelationship between activation of matrix metalloproteinases and mitochondrial dysfunction in the development of diabetic retinopathy.
Santos JM, Tewari S, Lin JY, Kowluru RA
Biochemical and biophysical research communications438(4)760-4(2013 Sep)

Role of TGF-?1 and TNF-? in IL-1? mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease.
Roy S, Samanta K, Chakraborti T, Chowdhury A, Chakraborti S
Archives of biochemistry and biophysics513(1)61-9(2011 Sep)

Decreased expression of matrix metalloproteinase-9 and increased expression of tissue inhibitors of matrix metalloproteinase-1 in paratuberculosis-infected cattle in the ELISA-negative subclinical stage.
Wang X, Wang H, Aodon-geril, Shu Y, Momotani Y, Nagata R, Mori Y, Momotani E
Animal biotechnology22(1)44-9(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In diabetic retinopathy transcription of MMP-9 is regulated by AP-1 binding at both, proximal and distal sites of its promoter, and acetylation of c-Jun and c-Fos subunits is important in its regulation.
Title: Molecular Mechanism of Transcriptional Regulation of Matrix Metalloproteinase-9 in Diabetic Retinopathy.

These data demonstrate that serum neutrophil haptoglobin-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins.
Title: Characterization of the contributions of Hp-MMP 9 to the serum acute phase protein response of lipopolysaccharide challenged calves.

Activation of cytosolic MMP-9 and MMP-2 was investigated in the retinal endothelial cells incubated in high glucose for 6-96 h, and correlated with their mitochondrial accumulation and mitochondrial damage.
Title: Interrelationship between activation of matrix metalloproteinases and mitochondrial dysfunction in the development of diabetic retinopathy.

Role of TGF-beta1 and TNF-alpha in IL-1beta mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease.
Title: Role of TGF-?1 and TNF-? in IL-1? mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease.

Decreased MMP-9 and increased TIMP-1 expression were found in peripheral blood cells from Mycobacterium avium subsp. paratuberculosis (Map)-infected cattle after stimulation with Map lysate and Map purified protein derivative than in control cattle.
Title: Decreased expression of matrix metalloproteinase-9 and increased expression of tissue inhibitors of matrix metalloproteinase-1 in paratuberculosis-infected cattle in the ELISA-negative subclinical stage.

The following MMP9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41786	NM_174744.2 Latest version!	Bos taurus matrix metallopeptidase 9 (MMP9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MMP9 ( NM_174744.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777169.1 Bos taurus matrix metallopeptidase 9 (MMP9), mRNA.

Price & Availability↑

CloneID	OBa41786
Clone ID Related Accession (Same CDS sequence)	NM_174744.2
Accession Version	NM_174744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	matrix metalloproteinase-9 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X78324.1. On Jun 3, 2003 this sequence version replaced NM_174744.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X78324.1, BC142430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145426, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCCCC TGCAGCCCTT GGTCCTGGCG CTCCTGGTGC TGGCTTGCTG CTCTGCTGTC 
CCCAGACGAC GCCAGCCCAC CGTTGTGGTC TTTCCAGGAG AACCACGAAC CAACCTCACC 
AACAGGCAGC TGGCAGAGGA ATACCTGTAC CGCTATGGCT ACACTCCTGG GGCAGAGCTG 
AGCGAGGACG GTCAGTCCCT GCAGCGAGCT CTGCTGCGCT TCCAGCGGCG CCTGTCCCTG 
CCCGAGACTG GCGAGCTGGA CAGCACCACC CTGAACGCCA TGCGAGCCCC GCGCTGCGGC 
GTCCCAGACG TGGGCAGATT CCAGACCTTT GAGGGCGAAC TCAAGTGGCA CCACCACAAC 
ATCACCTACT GGATCCAAAA TTACTCGGAA GACCTGCCGC GCGCCGTGAT CGACGACGCC 
TTTGCCCGCG CTTTCGCGCT CTGGAGCGCT GTGACGCCGC TCACCTTCAC TCGAGTGTAC 
GGCCCCGAAG CTGACATTGT CATCCAGTTT GGTGTTAGAG AGCACGGAGA TGGGTATCCC 
TTCGATGGGA AGAACGGGCT CCTGGCACAC GCCTTTCCGC CTGGCAAAGG CATTCAGGGA 
GATGCCCACT TCGACGATGA AGAGTTGTGG TCTCTGGGCA AAGGCGTTGT GATCCCGACC 
TACTTCGGAA ACGCGAAGGG CGCCGCCTGC CACTTCCCCT TCACCTTTGA GGGTCGCTCC 
TACTCCGCCT GCACCACGGA CGGCCGTTCC GACGACATGC TCTGGTGCAG CACCACCGCC 
GACTACGACG CCGACCGCCA GTTCGGCTTC TGCCCCAGCG AGAGACTCTA CACCCAGGAC 
GGCAATGCGG ACGGCAAGCC CTGCGTCTTC CCGTTCACCT TCCAGGGCCG CACCTACTCC 
GCCTGTACCT CCGATGGTCG CTCCGACGGC TACCGCTGGT GCGCCACCAC CGCCAACTAC 
GACCAGGACA AGCTCTACGG CTTCTGCCCG ACCCGAGTCG ATGCAACGGT GACCGGGGGC 
AACGCGGCGG GGGAGCTGTG CGTCTTCCCC TTCACCTTCC TGGGCAAGGA ATACTCGGCC 
TGCACCAGAG AGGGTCGCAA TGATGGGCAC CTCTGGTGCG CCACCACCTC CAACTTCGAC 
AAAGACAAGA AGTGGGGCTT CTGCCCGGAT CAAGGATACA GCCTGTTCCT TGTGGCCGCA 
CACGAGTTTG GCCACGCGCT GGGCTTAGAT CACACCTCCG TGCCAGAGGC GCTCATGTAC 
CCCATGTACA GATTCACAGA GGAGCACCCC CTGCATAGGG ACGATGTTCA GGGCATCCAG 
CATCTGTATG GTCCTCGCCC TGAGCCTGAA CCACGGCCTC CGACCACTAC CACCACTACC 
ACCACCGAAC CCCAGCCCAC CGCTCCCCCC ACGGTCTGCG TCACGGGGCC TCCCACCGCC 
CGCCCCTCAG AGGGTCCCAC TACTGGCCCC ACAGGGCCCC CGGCAGCTGG CCCTACGGGT 
CCTCCCACGG CTGGCCCTTC TGCGGCCCCG ACGGAGTCCC CGGATCCAGC GGAGGACGTC 
TGCAACGTGG ACATCTTCGA CGCCATCGCG GAGATTAGGA ACCGCTTGCA TTTCTTCAAG 
GCTGGGAAGT ACTGGAGACT TTCTGAGGGA GGGGGCCGCC GGGTGCAGGG TCCCTTCCTT 
GTCAAGAGCA AGTGGCCTGC GCTGCCCCGC AAGCTGGACT CCGCCTTCGA GGATCCGCTC 
ACCAAGAAGA TTTTCTTCTT CTCTGGGCGC CAAGTATGGG TGTACACCGG CGCGTCGTTG 
CTAGGCCCGA GGCGTCTGGA CAAGTTGGGC CTGGGCCCGG AAGTGGCCCA GGTCACCGGG 
GCCCTCCCGC GCCCTGAGGG TAAGGTGCTG CTGTTCAGCG GGCAGAGCTT CTGGAGGTTC 
GACGTGAAGA CACAGAAGGT GGATCCCCAG AGCGTCACCC CCGTGGACCA GATGTTCCCC 
GGGGTGCCCA TTAGCACGCA CGACATCTTT CAGTACCAAG AGAAAGCTTA CTTCTGCCAG 
GATCACTTCT ACTGGCGCGT GAGTTCCCAG AATGAGGTGA ATCAGGTGGA CTATGTGGGC 
TACGTGACCT TCGACCTCCT GAAGTGCCCT GAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777169.1
CDS	7..2145
Translation	MSPLQPLVLALLVLACCSAVPRRRQPTVVVFPGEPRTNLTNRQLAEEYLYRYGYTPGAELSEDGQSLQRALLRFQRRLSLPETGELDSTTLNAMRAPRCGVPDVGRFQTFEGELKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYGPEADIVIQFGVREHGDGYPFDGKNGLLAHAFPPGKGIQGDAHFDDEELWSLGKGVVIPTYFGNAKGAACHFPFTFEGRSYSACTTDGRSDDMLWCSTTADYDADRQFGFCPSERLYTQDGNADGKPCVFPFTFQGRTYSACTSDGRSDGYRWCATTANYDQDKLYGFCPTRVDATVTGGNAAGELCVFPFTFLGKEYSACTREGRNDGHLWCATTSNFDKDKKWGFCPDQGYSLFLVAAHEFGHALGLDHTSVPEALMYPMYRFTEEHPLHRDDVQGIQHLYGPRPEPEPRPPTTTTTTTTEPQPTAPPTVCVTGPPTARPSEGPTTGPTGPPAAGPTGPPTAGPSAAPTESPDPAEDVCNVDIFDAIAEIRNRLHFFKAGKYWRLSEGGGRRVQGPFLVKSKWPALPRKLDSAFEDPLTKKIFFFSGRQVWVYTGASLLGPRRLDKLGLGPEVAQVTGALPRPEGKVLLFSGQSFWRFDVKTQKVDPQSVTPVDQMFPGVPISTHDIFQYQEKAYFCQDHFYWRVSSQNEVNQVDYVGYVTFDLLKCPED

Target ORF information:

RefSeq Version	NM_174744.2
Organism	Bos taurus(cattle)
Definition	Bos taurus matrix metallopeptidase 9 (MMP9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174744.2

ORF Insert Sequence:

ATGAGCCCCC TGCAGCCCTT GGTCCTGGCG CTCCTGGTGC TGGCTTGCTG CTCTGCTGTC 
CCCAGACGAC GCCAGCCCAC CGTTGTGGTC TTTCCAGGAG AACCACGAAC CAACCTCACC 
AACAGGCAGC TGGCAGAGGA ATACCTGTAC CGCTATGGCT ACACTCCTGG GGCAGAGCTG 
AGCGAGGACG GTCAGTCCCT GCAGCGAGCT CTGCTGCGCT TCCAGCGGCG CCTGTCCCTG 
CCCGAGACTG GCGAGCTGGA CAGCACCACC CTGAACGCCA TGCGAGCCCC GCGCTGCGGC 
GTCCCAGACG TGGGCAGATT CCAGACCTTT GAGGGCGAAC TCAAGTGGCA CCACCACAAC 
ATCACCTACT GGATCCAAAA TTACTCGGAA GACCTGCCGC GCGCCGTGAT CGACGACGCC 
TTTGCCCGCG CTTTCGCGCT CTGGAGCGCT GTGACGCCGC TCACCTTCAC TCGAGTGTAC 
GGCCCCGAAG CTGACATTGT CATCCAGTTT GGTGTTAGAG AGCACGGAGA TGGGTATCCC 
TTCGATGGGA AGAACGGGCT CCTGGCACAC GCCTTTCCGC CTGGCAAAGG CATTCAGGGA 
GATGCCCACT TCGACGATGA AGAGTTGTGG TCTCTGGGCA AAGGCGTTGT GATCCCGACC 
TACTTCGGAA ACGCGAAGGG CGCCGCCTGC CACTTCCCCT TCACCTTTGA GGGTCGCTCC 
TACTCCGCCT GCACCACGGA CGGCCGTTCC GACGACATGC TCTGGTGCAG CACCACCGCC 
GACTACGACG CCGACCGCCA GTTCGGCTTC TGCCCCAGCG AGAGACTCTA CACCCAGGAC 
GGCAATGCGG ACGGCAAGCC CTGCGTCTTC CCGTTCACCT TCCAGGGCCG CACCTACTCC 
GCCTGTACCT CCGATGGTCG CTCCGACGGC TACCGCTGGT GCGCCACCAC CGCCAACTAC 
GACCAGGACA AGCTCTACGG CTTCTGCCCG ACCCGAGTCG ATGCAACGGT GACCGGGGGC 
AACGCGGCGG GGGAGCTGTG CGTCTTCCCC TTCACCTTCC TGGGCAAGGA ATACTCGGCC 
TGCACCAGAG AGGGTCGCAA TGATGGGCAC CTCTGGTGCG CCACCACCTC CAACTTCGAC 
AAAGACAAGA AGTGGGGCTT CTGCCCGGAT CAAGGATACA GCCTGTTCCT TGTGGCCGCA 
CACGAGTTTG GCCACGCGCT GGGCTTAGAT CACACCTCCG TGCCAGAGGC GCTCATGTAC 
CCCATGTACA GATTCACAGA GGAGCACCCC CTGCATAGGG ACGATGTTCA GGGCATCCAG 
CATCTGTATG GTCCTCGCCC TGAGCCTGAA CCACGGCCTC CGACCACTAC CACCACTACC 
ACCACCGAAC CCCAGCCCAC CGCTCCCCCC ACGGTCTGCG TCACGGGGCC TCCCACCGCC 
CGCCCCTCAG AGGGTCCCAC TACTGGCCCC ACAGGGCCCC CGGCAGCTGG CCCTACGGGT 
CCTCCCACGG CTGGCCCTTC TGCGGCCCCG ACGGAGTCCC CGGATCCAGC GGAGGACGTC 
TGCAACGTGG ACATCTTCGA CGCCATCGCG GAGATTAGGA ACCGCTTGCA TTTCTTCAAG 
GCTGGGAAGT ACTGGAGACT TTCTGAGGGA GGGGGCCGCC GGGTGCAGGG TCCCTTCCTT 
GTCAAGAGCA AGTGGCCTGC GCTGCCCCGC AAGCTGGACT CCGCCTTCGA GGATCCGCTC 
ACCAAGAAGA TTTTCTTCTT CTCTGGGCGC CAAGTATGGG TGTACACCGG CGCGTCGTTG 
CTAGGCCCGA GGCGTCTGGA CAAGTTGGGC CTGGGCCCGG AAGTGGCCCA GGTCACCGGG 
GCCCTCCCGC GCCCTGAGGG TAAGGTGCTG CTGTTCAGCG GGCAGAGCTT CTGGAGGTTC 
GACGTGAAGA CACAGAAGGT GGATCCCCAG AGCGTCACCC CCGTGGACCA GATGTTCCCC 
GGGGTGCCCA TTAGCACGCA CGACATCTTT CAGTACCAAG AGAAAGCTTA CTTCTGCCAG 
GATCACTTCT ACTGGCGCGT GAGTTCCCAG AATGAGGTGA ATCAGGTGGA CTATGTGGGC 
TACGTGACCT TCGACCTCCT GAAGTGCCCT GAGGACTAG