EIF4G1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following EIF4G1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4G1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219179 XM_010801318.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219171 XM_024995308.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219172 XM_024995312.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219173 XM_015472316.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219174 XM_010801319.3
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219175 XM_024995325.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219176 XM_024995329.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219177 XM_024995330.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219178 XM_024995332.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa45811 XM_015472316.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45811 XM_010801318.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45811 XM_015462064.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45811 XM_015462063.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45812 XM_010801319.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45812 XM_015462065.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45813 XM_015462066.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45813 XM_010801320.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219179
    Clone ID Related Accession (Same CDS sequence) XM_010801318.2
    Accession Version XM_010801318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGGA AGACGATCCG AATTCGAGAC 
    CCAAACCAAG GAGGGAAGGA TATCACGGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC
    ACACCCACAC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AGGCTAATGG GGAGACACCC
    CAGGTTGCTG TCGTCGTCCG GCCAGATGAC CGGTCGCAGG GAGCAATCAT TGGGGAGCGG
    CCAGGGCTGC CTGGCCCAGA GCACAGCCCT TCAGAATCCC AGCCTTCATC ACCTTCTCCA
    ACCCCATCAC CACCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT
    GTTCCTGGGG ACACAATGAC AACAGGGATG ATACAGACGT CTGTAGAAGA ATCAACCCCC
    ACGCCCCCTG AAAGTGGGGA GCCGTGTTGC CTCTCTCCAG AACCCACTCC CCTTGCTGAA
    CCCATATTGG AAGTAGAAGT GACGCTGAGC AAACCAGTTC CAGAATCTGA GTTCTCTTCC
    AGTCCTCTCC AGGTTCCCAG CCCCCTGGCA TCTCACAAGG TGGAAATTCT TCCTGAGCCT
    AATGGCACAG TCCCATCTGA GAATTTGGAA CCAGAGGTGG AGTCAAGCCC AGAGCTTGCC
    CCTCTCCCTC CTCCAGCTTG TCCCTCTGAA TCCCCCATGC CTATTGCTCC AACTGCCCAA
    CCTGAGGAAC TACTCAACGG AGCCCCTTCA CCACCAGCTG TGGACTTAAG CCCAGTCAGT
    GAACCAGAGG AACAGGCCAA GGAGGCTATA GTGTCGGTGA CTCCCCCCAC TGTCCTTTCT
    GCTACCCCAG CTGTGGCTCC TCCAGTGGCT TCCCCTGCTC AGGAGGAGGA CATGGAGGAG
    GAGGAAGAAG AGGAAGAGGA AGGAGAAGCT GAGGGTGAGA AGGGAGGAGA GGAACCTCTC
    CCCCAAGAGA GCACCCCTGT CCCAGCCCAC CTATCCCAGA ATTCGGAGGT GGCAGTAGCC
    ACCCAAGTGG CAGTGTCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT CAATAAGAAG
    GAGGCTGTAG GAGACCTTCT AGATGCCTTC AAGGAGGCGA ACCCAGGGGT ACCAGAGGTG
    GAAAATCAGC CTCCTGCAGG CTGCAATCCC AGCCCAGAGC CTGAGGGCAG CAGTGTGCCC
    CTGCGGTCTG AGGAAGGAGA GGAGACCTGG GACTCAAAGG AAGACAAGAT TCAAAATGCA
    GAGAATATCC AGCCAGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA
    AACCTTGAGG AGAAAAAGCG TTATGACCGA GAGTTCCTGC TTGGCTTTCA GTTCATCTTT
    GCCAGTATGC AGAAGCCTGA GGGATTGCCC CACATCAGTG ATGTGGTGTT GGATAAGGCC
    AATAAAACAC CATTGCGACC GCTGGACCCC TCTAGACTTC AGGGCATAAA TTGTGGCCCA
    GACTTCACTC CGTCTTTTGC CAACCTTGGC CGACCAGCCC TTAGCAACCG TGGGCCCCCG
    AGGGGTGGGC CAGGTGGGGA GCTGCCCCGA GGGCCGCAGG CTGGCCTGGG ACCCCGGCGA
    TCTCAGCAGG GCCCCCGAAA GGAACCACGC AAGATCATTG CCACAGTGTC AATGACCGAA
    GATATAAAGC TGAACAAAGC AGAAAAGGCC TGGAAACCCA GTAGCAAGCG GACGGCGGCT
    GATAAGGACC GAGGAGAAGA GGATGCTGAT GGCAGCAAAA CCCAGGACCT GTTCCGCAGG
    GTGCGCTCCA TCTTGAATAA GCTGACACCC CAGATGTTCC AGCAGCTGAT GAAGCAGGTG
    ACGCAGCTAG CCATTGATAC CGAGGAACGT CTCAAAGGGG TCATTGACCT CATCTTCGAG
    AAGGCCATTT CAGAACCCAA CTTCTCCGTG GCCTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTGACTG TGAACTTCCG AAAACTGTTG
    TTAAATCGAT GTCAGAAGGA GTTTGAAAAA GACAAAGATG ATGATGAGGT TTTTGAGAAG
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAGGAAC GGGGACGCCT GAAAGAAGAG
    CTGGAAGAGG CTCGAGACAT AGCCCGGCGG CGCTCGTTAG GGAATATCAA GTTTATCGGG
    GAGTTGTTCA AATTGAAGAT GTTAACAGAG GCAATCATGC ATGACTGTGT GGTTAAACTA
    CTTAAGAACC ATGATGAAGA GTCCCTTGAA TGCCTTTGCC GTCTGCTCAC CACCATTGGC
    AAAGACCTGG ACTTTGAGAA GGCCAAGCCC CGGATGGATC AGTATTTCAA CCAGATGGAA
    AAAATCATTA AGGAAAAGAA GACTTCATCC CGGATCCGCT TTATGCTGCA GGATGTGCTG
    GATCTGCGAA AGAGCAACTG GGTGCCACGC CGTGGGGACC AGGGGCCCAA GACAATTGAC
    CAAATCCACA AGGAAGCTGA GATGGAGGAG CATCGGGAGC ACATAAAAGT GCAGCAGCTA
    ATGGCCAAGG GCAGCGACAA GCGTCGGGGT GGCCCTCCAG GCCCACCCAT CAGCCGTGGC
    CTTCCACTTG TGGATGATGG TGGCTGGAAC ACAGTACCCA TCAGCAAGGG CAGCCGCCCT
    ATTGACACTT CTCGACTCAC TAAGATCACA AAGCCTGGCT CCATTGATTC TAATAACCAG
    CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC
    AAGCCCTCCG ATGCAGCATC AGAAGCTGCT CGTCCAGCTA CTAGTACCTT GAATCGCTTC
    TCAGCCCTTC AACAAGCAGT ACCAACAGAA AGCACAGACA ACAGACGAGT GGTGCAGAGG
    AGTAGCTTGA GCCGGGAACG AGGTGAGAAA GCTGGGGACC GGGGAGACCG CCTAGAGCGG
    AGTGAACGGG GAGGTGACCG TGGTGACCGG CTTGATCGCG CCCGGACACC CGCCACCAAG
    CGGAGCTTCA GCAAGGAAGT GGAGGAACGG AGTAGAGAGC GGCCCTCTCA GCCTGATGGA
    CTACGCAAGG CAGCTAGCCT CACGGAGGAT CGGGACCGCG GGCGGGAAGC TGTGAAACGA
    GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG
    AAGAAATCCA GGGCCATCAT TGAGGAGTAC CTCCATCTCA ATGACATGAA GGAGGCAGTG
    CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC CTGCTCTTTA TCTTTGTACG GCACGGCATC
    GAGTCCACGC TGGAGCGCAG CGCCATCACG CGGGAGCACA TGGGCCGGCT GCTGCACCAG
    CTGCTCTGTG CTGGGCACCT CTCCACTGCT CAGTACTACC AAGGGCTATA TGAAATTCTA
    GAGTTGGCTG AAGACATGGA AATTGACATC CCTCATGTGT GGCTCTACCT AGCAGAATTG
    ATAACGCCCA TTCTGCAGGA AGGTGGGGTG CCAATGGGGG AGCTGTTCAG GGAAATAACA
    AAACCTCTGA GACCCCTGGG CAAGGCTGCT TCCCTGTTGC TGGAGATCCT GGGGCTCCTG
    TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG
    AAGGAATTTC TGCCTGAAGG CCAGGATGTC AGTGCCTTTG TCACTGCGCA GAAGGTGGAG
    TATACCCTGG GAGAGGAGTT AGAAGCCCCA GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG
    CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG GGCAGCAGTA ACCAGCGGGT GTTTGACTGG
    ATAGAGGCCA ACCTGAACGA GCAGCAGGTG ACATCCAACA CATTAGTTCG AGCCCTTATG
    ACAACTGTCT GCTATTCTGC AATTATCTTT GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG
    CTGAAAGCAC GAGCGAAACT GCTACAGAAA TACCTGTGTG ACGAGCAGAA GGAGCTGCAG
    GCGCTCTATG CCCTCCAGGC CCTTGTAGTG ACCTTAGACC AGCCCCCTAA CCTGCTTGGG
    ATGTTCTTCG ATGCGCTGTA TGACGAGGAC GTGGTGAAGG AGGACGCCTT CTATAGCTGG
    GAGAGTAGCA AGGACCCTGC TGAGCAGCAG GGCAAGGGCG TGGCCCTAAA ATCTGTCACG
    ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799620.2
    CDS471..4784
    Translation

    Target ORF information:

    RefSeq Version XM_010801318.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGGA AGACGATCCG AATTCGAGAC 
    CCAAACCAAG GAGGGAAGGA TATCACGGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC
    ACACCCACAC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AGGCTAATGG GGAGACACCC
    CAGGTTGCTG TCGTCGTCCG GCCAGATGAC CGGTCGCAGG GAGCAATCAT TGGGGAGCGG
    CCAGGGCTGC CTGGCCCAGA GCACAGCCCT TCAGAATCCC AGCCTTCATC ACCTTCTCCA
    ACCCCATCAC CACCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT
    GTTCCTGGGG ACACAATGAC AACAGGGATG ATACAGACGT CTGTAGAAGA ATCAACCCCC
    ACGCCCCCTG AAAGTGGGGA GCCGTGTTGC CTCTCTCCAG AACCCACTCC CCTTGCTGAA
    CCCATATTGG AAGTAGAAGT GACGCTGAGC AAACCAGTTC CAGAATCTGA GTTCTCTTCC
    AGTCCTCTCC AGGTTCCCAG CCCCCTGGCA TCTCACAAGG TGGAAATTCT TCCTGAGCCT
    AATGGCACAG TCCCATCTGA GAATTTGGAA CCAGAGGTGG AGTCAAGCCC AGAGCTTGCC
    CCTCTCCCTC CTCCAGCTTG TCCCTCTGAA TCCCCCATGC CTATTGCTCC AACTGCCCAA
    CCTGAGGAAC TACTCAACGG AGCCCCTTCA CCACCAGCTG TGGACTTAAG CCCAGTCAGT
    GAACCAGAGG AACAGGCCAA GGAGGCTATA GTGTCGGTGA CTCCCCCCAC TGTCCTTTCT
    GCTACCCCAG CTGTGGCTCC TCCAGTGGCT TCCCCTGCTC AGGAGGAGGA CATGGAGGAG
    GAGGAAGAAG AGGAAGAGGA AGGAGAAGCT GAGGGTGAGA AGGGAGGAGA GGAACCTCTC
    CCCCAAGAGA GCACCCCTGT CCCAGCCCAC CTATCCCAGA ATTCGGAGGT GGCAGTAGCC
    ACCCAAGTGG CAGTGTCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT CAATAAGAAG
    GAGGCTGTAG GAGACCTTCT AGATGCCTTC AAGGAGGCGA ACCCAGGGGT ACCAGAGGTG
    GAAAATCAGC CTCCTGCAGG CTGCAATCCC AGCCCAGAGC CTGAGGGCAG CAGTGTGCCC
    CTGCGGTCTG AGGAAGGAGA GGAGACCTGG GACTCAAAGG AAGACAAGAT TCAAAATGCA
    GAGAATATCC AGCCAGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA
    AACCTTGAGG AGAAAAAGCG TTATGACCGA GAGTTCCTGC TTGGCTTTCA GTTCATCTTT
    GCCAGTATGC AGAAGCCTGA GGGATTGCCC CACATCAGTG ATGTGGTGTT GGATAAGGCC
    AATAAAACAC CATTGCGACC GCTGGACCCC TCTAGACTTC AGGGCATAAA TTGTGGCCCA
    GACTTCACTC CGTCTTTTGC CAACCTTGGC CGACCAGCCC TTAGCAACCG TGGGCCCCCG
    AGGGGTGGGC CAGGTGGGGA GCTGCCCCGA GGGCCGCAGG CTGGCCTGGG ACCCCGGCGA
    TCTCAGCAGG GCCCCCGAAA GGAACCACGC AAGATCATTG CCACAGTGTC AATGACCGAA
    GATATAAAGC TGAACAAAGC AGAAAAGGCC TGGAAACCCA GTAGCAAGCG GACGGCGGCT
    GATAAGGACC GAGGAGAAGA GGATGCTGAT GGCAGCAAAA CCCAGGACCT GTTCCGCAGG
    GTGCGCTCCA TCTTGAATAA GCTGACACCC CAGATGTTCC AGCAGCTGAT GAAGCAGGTG
    ACGCAGCTAG CCATTGATAC CGAGGAACGT CTCAAAGGGG TCATTGACCT CATCTTCGAG
    AAGGCCATTT CAGAACCCAA CTTCTCCGTG GCCTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTGACTG TGAACTTCCG AAAACTGTTG
    TTAAATCGAT GTCAGAAGGA GTTTGAAAAA GACAAAGATG ATGATGAGGT TTTTGAGAAG
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAGGAAC GGGGACGCCT GAAAGAAGAG
    CTGGAAGAGG CTCGAGACAT AGCCCGGCGG CGCTCGTTAG GGAATATCAA GTTTATCGGG
    GAGTTGTTCA AATTGAAGAT GTTAACAGAG GCAATCATGC ATGACTGTGT GGTTAAACTA
    CTTAAGAACC ATGATGAAGA GTCCCTTGAA TGCCTTTGCC GTCTGCTCAC CACCATTGGC
    AAAGACCTGG ACTTTGAGAA GGCCAAGCCC CGGATGGATC AGTATTTCAA CCAGATGGAA
    AAAATCATTA AGGAAAAGAA GACTTCATCC CGGATCCGCT TTATGCTGCA GGATGTGCTG
    GATCTGCGAA AGAGCAACTG GGTGCCACGC CGTGGGGACC AGGGGCCCAA GACAATTGAC
    CAAATCCACA AGGAAGCTGA GATGGAGGAG CATCGGGAGC ACATAAAAGT GCAGCAGCTA
    ATGGCCAAGG GCAGCGACAA GCGTCGGGGT GGCCCTCCAG GCCCACCCAT CAGCCGTGGC
    CTTCCACTTG TGGATGATGG TGGCTGGAAC ACAGTACCCA TCAGCAAGGG CAGCCGCCCT
    ATTGACACTT CTCGACTCAC TAAGATCACA AAGCCTGGCT CCATTGATTC TAATAACCAG
    CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC
    AAGCCCTCCG ATGCAGCATC AGAAGCTGCT CGTCCAGCTA CTAGTACCTT GAATCGCTTC
    TCAGCCCTTC AACAAGCAGT ACCAACAGAA AGCACAGACA ACAGACGAGT GGTGCAGAGG
    AGTAGCTTGA GCCGGGAACG AGGTGAGAAA GCTGGGGACC GGGGAGACCG CCTAGAGCGG
    AGTGAACGGG GAGGTGACCG TGGTGACCGG CTTGATCGCG CCCGGACACC CGCCACCAAG
    CGGAGCTTCA GCAAGGAAGT GGAGGAACGG AGTAGAGAGC GGCCCTCTCA GCCTGATGGA
    CTACGCAAGG CAGCTAGCCT CACGGAGGAT CGGGACCGCG GGCGGGAAGC TGTGAAACGA
    GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG
    AAGAAATCCA GGGCCATCAT TGAGGAGTAC CTCCATCTCA ATGACATGAA GGAGGCAGTG
    CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC CTGCTCTTTA TCTTTGTACG GCACGGCATC
    GAGTCCACGC TGGAGCGCAG CGCCATCACG CGGGAGCACA TGGGCCGGCT GCTGCACCAG
    CTGCTCTGTG CTGGGCACCT CTCCACTGCT CAGTACTACC AAGGGCTATA TGAAATTCTA
    GAGTTGGCTG AAGACATGGA AATTGACATC CCTCATGTGT GGCTCTACCT AGCAGAATTG
    ATAACGCCCA TTCTGCAGGA AGGTGGGGTG CCAATGGGGG AGCTGTTCAG GGAAATAACA
    AAACCTCTGA GACCCCTGGG CAAGGCTGCT TCCCTGTTGC TGGAGATCCT GGGGCTCCTG
    TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG
    AAGGAATTTC TGCCTGAAGG CCAGGATGTC AGTGCCTTTG TCACTGCGCA GAAGGTGGAG
    TATACCCTGG GAGAGGAGTT AGAAGCCCCA GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG
    CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG GGCAGCAGTA ACCAGCGGGT GTTTGACTGG
    ATAGAGGCCA ACCTGAACGA GCAGCAGGTG ACATCCAACA CATTAGTTCG AGCCCTTATG
    ACAACTGTCT GCTATTCTGC AATTATCTTT GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG
    CTGAAAGCAC GAGCGAAACT GCTACAGAAA TACCTGTGTG ACGAGCAGAA GGAGCTGCAG
    GCGCTCTATG CCCTCCAGGC CCTTGTAGTG ACCTTAGACC AGCCCCCTAA CCTGCTTGGG
    ATGTTCTTCG ATGCGCTGTA TGACGAGGAC GTGGTGAAGG AGGACGCCTT CTATAGCTGG
    GAGAGTAGCA AGGACCCTGC TGAGCAGCAG GGCAAGGGCG TGGCCCTAAA ATCTGTCACG
    ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219171
    Clone ID Related Accession (Same CDS sequence) XM_024995308.1
    Accession Version XM_024995308.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGC AGGCTGGCCT GGGACCCCGG CGATCTCAGC AGGGCCCCCG AAAGGAACCA
    CGCAAGATCA TTGCCACAGT GTCAATGACC GAAGATATAA AGCTGAACAA AGCAGAAAAG
    GCCTGGAAAC CCAGTAGCAA GCGGACGGCG GCTGATAAGG ACCGAGGAGA AGAGGATGCT
    GATGGCAGCA AAACCCAGGA CCTGTTCCGC AGGGTGCGCT CCATCTTGAA TAAGCTGACA
    CCCCAGATGT TCCAGCAGCT GATGAAGCAG GTGACGCAGC TAGCCATTGA TACCGAGGAA
    CGTCTCAAAG GGGTCATTGA CCTCATCTTC GAGAAGGCCA TTTCAGAACC CAACTTCTCC
    GTGGCCTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG
    CCAACAGTGA CTGTGAACTT CCGAAAACTG TTGTTAAATC GATGTCAGAA GGAGTTTGAA
    AAAGACAAAG ATGATGATGA GGTTTTTGAG AAGAAGCAAA AAGAGATGGA TGAAGCTGCT
    ACGGCAGAGG AACGGGGACG CCTGAAAGAA GAGCTGGAAG AGGCTCGAGA CATAGCCCGG
    CGGCGCTCGT TAGGGAATAT CAAGTTTATC GGGGAGTTGT TCAAATTGAA GATGTTAACA
    GAGGCAATCA TGCATGACTG TGTGGTTAAA CTACTTAAGA ACCATGATGA AGAGTCCCTT
    GAATGCCTTT GCCGTCTGCT CACCACCATT GGCAAAGACC TGGACTTTGA GAAGGCCAAG
    CCCCGGATGG ATCAGTATTT CAACCAGATG GAAAAAATCA TTAAGGAAAA GAAGACTTCA
    TCCCGGATCC GCTTTATGCT GCAGGATGTG CTGGATCTGC GAAAGAGCAA CTGGGTGCCA
    CGCCGTGGGG ACCAGGGGCC CAAGACAATT GACCAAATCC ACAAGGAAGC TGAGATGGAG
    GAGCATCGGG AGCACATAAA AGTGCAGCAG CTAATGGCCA AGGGCAGCGA CAAGCGTCGG
    GGTGGCCCTC CAGGCCCACC CATCAGCCGT GGCCTTCCAC TTGTGGATGA TGGTGGCTGG
    AACACAGTAC CCATCAGCAA GGGCAGCCGC CCTATTGACA CTTCTCGACT CACTAAGATC
    ACAAAGCCTG GCTCCATTGA TTCTAATAAC CAGCTGTTTG CACCTGGAGG CCGATTGAGC
    TGGGGCAAGG GTAGCAGTGG AGGCTCAGGA GCCAAGCCCT CCGATGCAGC ATCAGAAGCT
    GCTCGTCCAG CTACTAGTAC CTTGAATCGC TTCTCAGCCC TTCAACAAGC AGTACCAACA
    GAAAGCACAG ACAACAGACG AGTGGTGCAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG
    AAAGCTGGGG ACCGGGGAGA CCGCCTAGAG CGGAGTGAAC GGGGAGGTGA CCGTGGTGAC
    CGGCTTGATC GCGCCCGGAC ACCCGCCACC AAGCGGAGCT TCAGCAAGGA AGTGGAGGAA
    CGGAGTAGAG AGCGGCCCTC TCAGCCTGAT GGACTACGCA AGGCAGCTAG CCTCACGGAG
    GATCGGGACC GCGGGCGGGA AGCTGTGAAA CGAGAAGCTG CCCTCCCCCC GGTGAGTCCC
    CCGAAGGCTG CACTCTCTGA AGAGGAGCTG GAGAAGAAAT CCAGGGCCAT CATTGAGGAG
    TACCTCCATC TCAATGACAT GAAGGAGGCA GTGCAGTGCG TGCAGGAGCT GGCTTCGCCC
    TCCCTGCTCT TTATCTTTGT ACGGCACGGC ATCGAGTCCA CGCTGGAGCG CAGCGCCATC
    ACGCGGGAGC ACATGGGCCG GCTGCTGCAC CAGCTGCTCT GTGCTGGGCA CCTCTCCACT
    GCTCAGTACT ACCAAGGGCT ATATGAAATT CTAGAGTTGG CTGAAGACAT GGAAATTGAC
    ATCCCTCATG TGTGGCTCTA CCTAGCAGAA TTGATAACGC CCATTCTGCA GGAAGGTGGG
    GTGCCAATGG GGGAGCTGTT CAGGGAAATA ACAAAACCTC TGAGACCCCT GGGCAAGGCT
    GCTTCCCTGT TGCTGGAGAT CCTGGGGCTC CTGTGCAAAA GCATGGGTCC TAAAAAGGTG
    GGGACGCTGT GGCGAGAGGC TGGACTCAGC TGGAAGGAAT TTCTGCCTGA AGGCCAGGAT
    GTCAGTGCCT TTGTCACTGC GCAGAAGGTG GAGTATACCC TGGGAGAGGA GTTAGAAGCC
    CCAGGCCAGA GGTTGCTGTC CTTTGAGGAG CTGCGCAGGC AGCTGGAGAA GCTGCTGCAG
    GAGGGCAGCA GTAACCAGCG GGTGTTTGAC TGGATAGAGG CCAACCTGAA CGAGCAGCAG
    GTGACATCCA ACACATTAGT TCGAGCCCTT ATGACAACTG TCTGCTATTC TGCAATTATC
    TTTGAGTCTT CTCTCCGAGT GGATGTTGCA GTGCTGAAAG CACGAGCGAA ACTGCTACAG
    AAATACCTGT GTGACGAGCA GAAGGAGCTG CAGGCGCTCT ATGCCCTCCA GGCCCTTGTA
    GTGACCTTAG ACCAGCCCCC TAACCTGCTT GGGATGTTCT TCGATGCGCT GTATGACGAG
    GACGTGGTGA AGGAGGACGC CTTCTATAGC TGGGAGAGTA GCAAGGACCC TGCTGAGCAG
    CAGGGCAAGG GCGTGGCCCT AAAATCTGTC ACGACTTTTT TCAAGTGGCT TCGTGAGGCG
    GAGGAGGAGG AGTCTGACCA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851076.1
    CDS248..5074
    Translation

    Target ORF information:

    RefSeq Version XM_024995308.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995308.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGC AGGCTGGCCT GGGACCCCGG CGATCTCAGC AGGGCCCCCG AAAGGAACCA
    CGCAAGATCA TTGCCACAGT GTCAATGACC GAAGATATAA AGCTGAACAA AGCAGAAAAG
    GCCTGGAAAC CCAGTAGCAA GCGGACGGCG GCTGATAAGG ACCGAGGAGA AGAGGATGCT
    GATGGCAGCA AAACCCAGGA CCTGTTCCGC AGGGTGCGCT CCATCTTGAA TAAGCTGACA
    CCCCAGATGT TCCAGCAGCT GATGAAGCAG GTGACGCAGC TAGCCATTGA TACCGAGGAA
    CGTCTCAAAG GGGTCATTGA CCTCATCTTC GAGAAGGCCA TTTCAGAACC CAACTTCTCC
    GTGGCCTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG
    CCAACAGTGA CTGTGAACTT CCGAAAACTG TTGTTAAATC GATGTCAGAA GGAGTTTGAA
    AAAGACAAAG ATGATGATGA GGTTTTTGAG AAGAAGCAAA AAGAGATGGA TGAAGCTGCT
    ACGGCAGAGG AACGGGGACG CCTGAAAGAA GAGCTGGAAG AGGCTCGAGA CATAGCCCGG
    CGGCGCTCGT TAGGGAATAT CAAGTTTATC GGGGAGTTGT TCAAATTGAA GATGTTAACA
    GAGGCAATCA TGCATGACTG TGTGGTTAAA CTACTTAAGA ACCATGATGA AGAGTCCCTT
    GAATGCCTTT GCCGTCTGCT CACCACCATT GGCAAAGACC TGGACTTTGA GAAGGCCAAG
    CCCCGGATGG ATCAGTATTT CAACCAGATG GAAAAAATCA TTAAGGAAAA GAAGACTTCA
    TCCCGGATCC GCTTTATGCT GCAGGATGTG CTGGATCTGC GAAAGAGCAA CTGGGTGCCA
    CGCCGTGGGG ACCAGGGGCC CAAGACAATT GACCAAATCC ACAAGGAAGC TGAGATGGAG
    GAGCATCGGG AGCACATAAA AGTGCAGCAG CTAATGGCCA AGGGCAGCGA CAAGCGTCGG
    GGTGGCCCTC CAGGCCCACC CATCAGCCGT GGCCTTCCAC TTGTGGATGA TGGTGGCTGG
    AACACAGTAC CCATCAGCAA GGGCAGCCGC CCTATTGACA CTTCTCGACT CACTAAGATC
    ACAAAGCCTG GCTCCATTGA TTCTAATAAC CAGCTGTTTG CACCTGGAGG CCGATTGAGC
    TGGGGCAAGG GTAGCAGTGG AGGCTCAGGA GCCAAGCCCT CCGATGCAGC ATCAGAAGCT
    GCTCGTCCAG CTACTAGTAC CTTGAATCGC TTCTCAGCCC TTCAACAAGC AGTACCAACA
    GAAAGCACAG ACAACAGACG AGTGGTGCAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG
    AAAGCTGGGG ACCGGGGAGA CCGCCTAGAG CGGAGTGAAC GGGGAGGTGA CCGTGGTGAC
    CGGCTTGATC GCGCCCGGAC ACCCGCCACC AAGCGGAGCT TCAGCAAGGA AGTGGAGGAA
    CGGAGTAGAG AGCGGCCCTC TCAGCCTGAT GGACTACGCA AGGCAGCTAG CCTCACGGAG
    GATCGGGACC GCGGGCGGGA AGCTGTGAAA CGAGAAGCTG CCCTCCCCCC GGTGAGTCCC
    CCGAAGGCTG CACTCTCTGA AGAGGAGCTG GAGAAGAAAT CCAGGGCCAT CATTGAGGAG
    TACCTCCATC TCAATGACAT GAAGGAGGCA GTGCAGTGCG TGCAGGAGCT GGCTTCGCCC
    TCCCTGCTCT TTATCTTTGT ACGGCACGGC ATCGAGTCCA CGCTGGAGCG CAGCGCCATC
    ACGCGGGAGC ACATGGGCCG GCTGCTGCAC CAGCTGCTCT GTGCTGGGCA CCTCTCCACT
    GCTCAGTACT ACCAAGGGCT ATATGAAATT CTAGAGTTGG CTGAAGACAT GGAAATTGAC
    ATCCCTCATG TGTGGCTCTA CCTAGCAGAA TTGATAACGC CCATTCTGCA GGAAGGTGGG
    GTGCCAATGG GGGAGCTGTT CAGGGAAATA ACAAAACCTC TGAGACCCCT GGGCAAGGCT
    GCTTCCCTGT TGCTGGAGAT CCTGGGGCTC CTGTGCAAAA GCATGGGTCC TAAAAAGGTG
    GGGACGCTGT GGCGAGAGGC TGGACTCAGC TGGAAGGAAT TTCTGCCTGA AGGCCAGGAT
    GTCAGTGCCT TTGTCACTGC GCAGAAGGTG GAGTATACCC TGGGAGAGGA GTTAGAAGCC
    CCAGGCCAGA GGTTGCTGTC CTTTGAGGAG CTGCGCAGGC AGCTGGAGAA GCTGCTGCAG
    GAGGGCAGCA GTAACCAGCG GGTGTTTGAC TGGATAGAGG CCAACCTGAA CGAGCAGCAG
    GTGACATCCA ACACATTAGT TCGAGCCCTT ATGACAACTG TCTGCTATTC TGCAATTATC
    TTTGAGTCTT CTCTCCGAGT GGATGTTGCA GTGCTGAAAG CACGAGCGAA ACTGCTACAG
    AAATACCTGT GTGACGAGCA GAAGGAGCTG CAGGCGCTCT ATGCCCTCCA GGCCCTTGTA
    GTGACCTTAG ACCAGCCCCC TAACCTGCTT GGGATGTTCT TCGATGCGCT GTATGACGAG
    GACGTGGTGA AGGAGGACGC CTTCTATAGC TGGGAGAGTA GCAAGGACCC TGCTGAGCAG
    CAGGGCAAGG GCGTGGCCCT AAAATCTGTC ACGACTTTTT TCAAGTGGCT TCGTGAGGCG
    GAGGAGGAGG AGTCTGACCA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219172
    Clone ID Related Accession (Same CDS sequence) XM_024995312.1
    Accession Version XM_024995312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC
    AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC
    TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT
    GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT
    CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG
    CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG
    GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA
    TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC
    CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC
    CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC
    CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG
    CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT
    GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC
    ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA
    AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG
    GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT
    CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA
    AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA
    GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG
    CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG
    AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG
    AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC
    CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC
    CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG
    CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT
    CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC
    CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG
    CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC
    AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA
    GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG
    ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT
    GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC
    GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG
    GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG
    GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851080.1
    CDS248..5071
    Translation

    Target ORF information:

    RefSeq Version XM_024995312.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC
    AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC
    TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT
    GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT
    CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG
    CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG
    GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA
    TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC
    CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC
    CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC
    CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG
    CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT
    GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC
    ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA
    AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG
    GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT
    CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA
    AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA
    GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG
    CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG
    AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG
    AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC
    CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC
    CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG
    CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT
    CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC
    CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG
    CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC
    AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA
    GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG
    ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT
    GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC
    GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG
    GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG
    GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219173
    Clone ID Related Accession (Same CDS sequence) XM_015472316.2
    Accession Version XM_015472316.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT
    CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC
    TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC
    CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT
    GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC
    ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA
    TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA
    GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT
    CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT
    CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC
    CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT
    CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA
    AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC
    ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG
    GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA
    GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG
    GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC
    AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG
    ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG
    TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA
    AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC
    CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG
    GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG
    TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG
    CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC
    CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC
    CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC
    CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC
    AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT
    GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG
    CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC
    AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA
    GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC
    ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG
    GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT
    TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA
    GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC
    TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA
    GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA
    CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT
    CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA
    GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA
    GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG
    AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA
    CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA
    TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC
    CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC
    AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT
    GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC
    TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG
    GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT
    CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG
    GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT
    AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC
    TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA
    AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327802.2
    CDS248..5053
    Translation

    Target ORF information:

    RefSeq Version XM_015472316.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472316.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT
    CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC
    TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC
    CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT
    GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC
    ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA
    TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA
    GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT
    CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT
    CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC
    CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT
    CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA
    AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC
    ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG
    GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA
    GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG
    GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC
    AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG
    ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG
    TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA
    AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC
    CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG
    GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG
    TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG
    CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC
    CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC
    CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC
    CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC
    AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT
    GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG
    CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC
    AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA
    GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC
    ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG
    GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT
    TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA
    GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC
    TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA
    GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA
    CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT
    CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA
    GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA
    GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG
    AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA
    CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA
    TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC
    CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC
    AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT
    GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC
    TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG
    GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT
    CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG
    GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT
    AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC
    TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA
    AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219174
    Clone ID Related Accession (Same CDS sequence) XM_010801319.3
    Accession Version XM_010801319.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT
    CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC
    TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC
    CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT
    GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC
    ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA
    TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA
    GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT
    CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT
    CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC
    CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT
    CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA
    AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC
    ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG
    GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA
    GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG
    GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC
    AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG
    ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG
    TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA
    AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC
    CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGGC TGGCCTGGGA
    CCCCGGCGAT CTCAGCAGGG CCCCCGAAAG GAACCACGCA AGATCATTGC CACAGTGTCA
    ATGACCGAAG ATATAAAGCT GAACAAAGCA GAAAAGGCCT GGAAACCCAG TAGCAAGCGG
    ACGGCGGCTG ATAAGGACCG AGGAGAAGAG GATGCTGATG GCAGCAAAAC CCAGGACCTG
    TTCCGCAGGG TGCGCTCCAT CTTGAATAAG CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAGGTGA CGCAGCTAGC CATTGATACC GAGGAACGTC TCAAAGGGGT CATTGACCTC
    ATCTTCGAGA AGGCCATTTC AGAACCCAAC TTCTCCGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACA GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAACTGTTGT TAAATCGATG TCAGAAGGAG TTTGAAAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG GGGACGCCTG
    AAAGAAGAGC TGGAAGAGGC TCGAGACATA GCCCGGCGGC GCTCGTTAGG GAATATCAAG
    TTTATCGGGG AGTTGTTCAA ATTGAAGATG TTAACAGAGG CAATCATGCA TGACTGTGTG
    GTTAAACTAC TTAAGAACCA TGATGAAGAG TCCCTTGAAT GCCTTTGCCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAGAAG GCCAAGCCCC GGATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA GGAAAAGAAG ACTTCATCCC GGATCCGCTT TATGCTGCAG
    GATGTGCTGG ATCTGCGAAA GAGCAACTGG GTGCCACGCC GTGGGGACCA GGGGCCCAAG
    ACAATTGACC AAATCCACAA GGAAGCTGAG ATGGAGGAGC ATCGGGAGCA CATAAAAGTG
    CAGCAGCTAA TGGCCAAGGG CAGCGACAAG CGTCGGGGTG GCCCTCCAGG CCCACCCATC
    AGCCGTGGCC TTCCACTTGT GGATGATGGT GGCTGGAACA CAGTACCCAT CAGCAAGGGC
    AGCCGCCCTA TTGACACTTC TCGACTCACT AAGATCACAA AGCCTGGCTC CATTGATTCT
    AATAACCAGC TGTTTGCACC TGGAGGCCGA TTGAGCTGGG GCAAGGGTAG CAGTGGAGGC
    TCAGGAGCCA AGCCCTCCGA TGCAGCATCA GAAGCTGCTC GTCCAGCTAC TAGTACCTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCAGTA CCAACAGAAA GCACAGACAA CAGACGAGTG
    GTGCAGAGGA GTAGCTTGAG CCGGGAACGA GGTGAGAAAG CTGGGGACCG GGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGTGACCGT GGTGACCGGC TTGATCGCGC CCGGACACCC
    GCCACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAACGGA GTAGAGAGCG GCCCTCTCAG
    CCTGATGGAC TACGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGCGG GCGGGAAGCT
    GTGAAACGAG AAGCTGCCCT CCCCCCGGTG AGTCCCCCGA AGGCTGCACT CTCTGAAGAG
    GAGCTGGAGA AGAAATCCAG GGCCATCATT GAGGAGTACC TCCATCTCAA TGACATGAAG
    GAGGCAGTGC AGTGCGTGCA GGAGCTGGCT TCGCCCTCCC TGCTCTTTAT CTTTGTACGG
    CACGGCATCG AGTCCACGCT GGAGCGCAGC GCCATCACGC GGGAGCACAT GGGCCGGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCACCTC TCCACTGCTC AGTACTACCA AGGGCTATAT
    GAAATTCTAG AGTTGGCTGA AGACATGGAA ATTGACATCC CTCATGTGTG GCTCTACCTA
    GCAGAATTGA TAACGCCCAT TCTGCAGGAA GGTGGGGTGC CAATGGGGGA GCTGTTCAGG
    GAAATAACAA AACCTCTGAG ACCCCTGGGC AAGGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGGCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAGGCTGGA
    CTCAGCTGGA AGGAATTTCT GCCTGAAGGC CAGGATGTCA GTGCCTTTGT CACTGCGCAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTTA GAAGCCCCAG GCCAGAGGTT GCTGTCCTTT
    GAGGAGCTGC GCAGGCAGCT GGAGAAGCTG CTGCAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTTGACTGGA TAGAGGCCAA CCTGAACGAG CAGCAGGTGA CATCCAACAC ATTAGTTCGA
    GCCCTTATGA CAACTGTCTG CTATTCTGCA ATTATCTTTG AGTCTTCTCT CCGAGTGGAT
    GTTGCAGTGC TGAAAGCACG AGCGAAACTG CTACAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTGCAGG CGCTCTATGC CCTCCAGGCC CTTGTAGTGA CCTTAGACCA GCCCCCTAAC
    CTGCTTGGGA TGTTCTTCGA TGCGCTGTAT GACGAGGACG TGGTGAAGGA GGACGCCTTC
    TATAGCTGGG AGAGTAGCAA GGACCCTGCT GAGCAGCAGG GCAAGGGCGT GGCCCTAAAA
    TCTGTCACGA CTTTTTTCAA GTGGCTTCGT GAGGCGGAGG AGGAGGAGTC TGACCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799621.2
    CDS234..5036
    Translation

    Target ORF information:

    RefSeq Version XM_010801319.3
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801319.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG
    GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT
    CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC
    TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC
    CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT
    GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC
    ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA
    TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC
    GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA
    GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT
    CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT
    CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC
    CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT
    CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA
    AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC
    ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG
    GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA
    GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG
    GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG
    CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG
    GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC
    AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG
    ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG
    TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT
    CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG
    TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA
    AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC
    CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGGC TGGCCTGGGA
    CCCCGGCGAT CTCAGCAGGG CCCCCGAAAG GAACCACGCA AGATCATTGC CACAGTGTCA
    ATGACCGAAG ATATAAAGCT GAACAAAGCA GAAAAGGCCT GGAAACCCAG TAGCAAGCGG
    ACGGCGGCTG ATAAGGACCG AGGAGAAGAG GATGCTGATG GCAGCAAAAC CCAGGACCTG
    TTCCGCAGGG TGCGCTCCAT CTTGAATAAG CTGACACCCC AGATGTTCCA GCAGCTGATG
    AAGCAGGTGA CGCAGCTAGC CATTGATACC GAGGAACGTC TCAAAGGGGT CATTGACCTC
    ATCTTCGAGA AGGCCATTTC AGAACCCAAC TTCTCCGTGG CCTATGCCAA CATGTGCCGC
    TGCCTCATGG CGCTGAAAGT GCCCACTACA GAAAAGCCAA CAGTGACTGT GAACTTCCGA
    AAACTGTTGT TAAATCGATG TCAGAAGGAG TTTGAAAAAG ACAAAGATGA TGATGAGGTT
    TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG GGGACGCCTG
    AAAGAAGAGC TGGAAGAGGC TCGAGACATA GCCCGGCGGC GCTCGTTAGG GAATATCAAG
    TTTATCGGGG AGTTGTTCAA ATTGAAGATG TTAACAGAGG CAATCATGCA TGACTGTGTG
    GTTAAACTAC TTAAGAACCA TGATGAAGAG TCCCTTGAAT GCCTTTGCCG TCTGCTCACC
    ACCATTGGCA AAGACCTGGA CTTTGAGAAG GCCAAGCCCC GGATGGATCA GTATTTCAAC
    CAGATGGAAA AAATCATTAA GGAAAAGAAG ACTTCATCCC GGATCCGCTT TATGCTGCAG
    GATGTGCTGG ATCTGCGAAA GAGCAACTGG GTGCCACGCC GTGGGGACCA GGGGCCCAAG
    ACAATTGACC AAATCCACAA GGAAGCTGAG ATGGAGGAGC ATCGGGAGCA CATAAAAGTG
    CAGCAGCTAA TGGCCAAGGG CAGCGACAAG CGTCGGGGTG GCCCTCCAGG CCCACCCATC
    AGCCGTGGCC TTCCACTTGT GGATGATGGT GGCTGGAACA CAGTACCCAT CAGCAAGGGC
    AGCCGCCCTA TTGACACTTC TCGACTCACT AAGATCACAA AGCCTGGCTC CATTGATTCT
    AATAACCAGC TGTTTGCACC TGGAGGCCGA TTGAGCTGGG GCAAGGGTAG CAGTGGAGGC
    TCAGGAGCCA AGCCCTCCGA TGCAGCATCA GAAGCTGCTC GTCCAGCTAC TAGTACCTTG
    AATCGCTTCT CAGCCCTTCA ACAAGCAGTA CCAACAGAAA GCACAGACAA CAGACGAGTG
    GTGCAGAGGA GTAGCTTGAG CCGGGAACGA GGTGAGAAAG CTGGGGACCG GGGAGACCGC
    CTAGAGCGGA GTGAACGGGG AGGTGACCGT GGTGACCGGC TTGATCGCGC CCGGACACCC
    GCCACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAACGGA GTAGAGAGCG GCCCTCTCAG
    CCTGATGGAC TACGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGCGG GCGGGAAGCT
    GTGAAACGAG AAGCTGCCCT CCCCCCGGTG AGTCCCCCGA AGGCTGCACT CTCTGAAGAG
    GAGCTGGAGA AGAAATCCAG GGCCATCATT GAGGAGTACC TCCATCTCAA TGACATGAAG
    GAGGCAGTGC AGTGCGTGCA GGAGCTGGCT TCGCCCTCCC TGCTCTTTAT CTTTGTACGG
    CACGGCATCG AGTCCACGCT GGAGCGCAGC GCCATCACGC GGGAGCACAT GGGCCGGCTG
    CTGCACCAGC TGCTCTGTGC TGGGCACCTC TCCACTGCTC AGTACTACCA AGGGCTATAT
    GAAATTCTAG AGTTGGCTGA AGACATGGAA ATTGACATCC CTCATGTGTG GCTCTACCTA
    GCAGAATTGA TAACGCCCAT TCTGCAGGAA GGTGGGGTGC CAATGGGGGA GCTGTTCAGG
    GAAATAACAA AACCTCTGAG ACCCCTGGGC AAGGCTGCTT CCCTGTTGCT GGAGATCCTG
    GGGCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAGGCTGGA
    CTCAGCTGGA AGGAATTTCT GCCTGAAGGC CAGGATGTCA GTGCCTTTGT CACTGCGCAG
    AAGGTGGAGT ATACCCTGGG AGAGGAGTTA GAAGCCCCAG GCCAGAGGTT GCTGTCCTTT
    GAGGAGCTGC GCAGGCAGCT GGAGAAGCTG CTGCAGGAGG GCAGCAGTAA CCAGCGGGTG
    TTTGACTGGA TAGAGGCCAA CCTGAACGAG CAGCAGGTGA CATCCAACAC ATTAGTTCGA
    GCCCTTATGA CAACTGTCTG CTATTCTGCA ATTATCTTTG AGTCTTCTCT CCGAGTGGAT
    GTTGCAGTGC TGAAAGCACG AGCGAAACTG CTACAGAAAT ACCTGTGTGA CGAGCAGAAG
    GAGCTGCAGG CGCTCTATGC CCTCCAGGCC CTTGTAGTGA CCTTAGACCA GCCCCCTAAC
    CTGCTTGGGA TGTTCTTCGA TGCGCTGTAT GACGAGGACG TGGTGAAGGA GGACGCCTTC
    TATAGCTGGG AGAGTAGCAA GGACCCTGCT GAGCAGCAGG GCAAGGGCGT GGCCCTAAAA
    TCTGTCACGA CTTTTTTCAA GTGGCTTCGT GAGGCGGAGG AGGAGGAGTC TGACCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219175
    Clone ID Related Accession (Same CDS sequence) XM_024995325.1
    Accession Version XM_024995325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA
    GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG
    ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC
    CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC
    TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG
    GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC
    CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG
    AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC
    CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC
    GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC
    CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC
    CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA
    ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT
    GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA
    GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT
    CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA
    TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA
    GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC
    AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG
    GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG
    GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA
    GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC
    CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG
    TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC
    CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT
    GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA
    GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA
    GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA
    AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG
    CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG
    CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT
    TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT
    GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC CTGGGACCCC GGCGATCTCA GCAGGGCCCC
    CGAAAGGAAC CACGCAAGAT CATTGCCACA GTGTCAATGA CCGAAGATAT AAAGCTGAAC
    AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC AAGCGGACGG CGGCTGATAA GGACCGAGGA
    GAAGAGGATG CTGATGGCAG CAAAACCCAG GACCTGTTCC GCAGGGTGCG CTCCATCTTG
    AATAAGCTGA CACCCCAGAT GTTCCAGCAG CTGATGAAGC AGGTGACGCA GCTAGCCATT
    GATACCGAGG AACGTCTCAA AGGGGTCATT GACCTCATCT TCGAGAAGGC CATTTCAGAA
    CCCAACTTCT CCGTGGCCTA TGCCAACATG TGCCGCTGCC TCATGGCGCT GAAAGTGCCC
    ACTACAGAAA AGCCAACAGT GACTGTGAAC TTCCGAAAAC TGTTGTTAAA TCGATGTCAG
    AAGGAGTTTG AAAAAGACAA AGATGATGAT GAGGTTTTTG AGAAGAAGCA AAAAGAGATG
    GATGAAGCTG CTACGGCAGA GGAACGGGGA CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA
    GACATAGCCC GGCGGCGCTC GTTAGGGAAT ATCAAGTTTA TCGGGGAGTT GTTCAAATTG
    AAGATGTTAA CAGAGGCAAT CATGCATGAC TGTGTGGTTA AACTACTTAA GAACCATGAT
    GAAGAGTCCC TTGAATGCCT TTGCCGTCTG CTCACCACCA TTGGCAAAGA CCTGGACTTT
    GAGAAGGCCA AGCCCCGGAT GGATCAGTAT TTCAACCAGA TGGAAAAAAT CATTAAGGAA
    AAGAAGACTT CATCCCGGAT CCGCTTTATG CTGCAGGATG TGCTGGATCT GCGAAAGAGC
    AACTGGGTGC CACGCCGTGG GGACCAGGGG CCCAAGACAA TTGACCAAAT CCACAAGGAA
    GCTGAGATGG AGGAGCATCG GGAGCACATA AAAGTGCAGC AGCTAATGGC CAAGGGCAGC
    GACAAGCGTC GGGGTGGCCC TCCAGGCCCA CCCATCAGCC GTGGCCTTCC ACTTGTGGAT
    GATGGTGGCT GGAACACAGT ACCCATCAGC AAGGGCAGCC GCCCTATTGA CACTTCTCGA
    CTCACTAAGA TCACAAAGCC TGGCTCCATT GATTCTAATA ACCAGCTGTT TGCACCTGGA
    GGCCGATTGA GCTGGGGCAA GGGTAGCAGT GGAGGCTCAG GAGCCAAGCC CTCCGATGCA
    GCATCAGAAG CTGCTCGTCC AGCTACTAGT ACCTTGAATC GCTTCTCAGC CCTTCAACAA
    GCAGTACCAA CAGAAAGCAC AGACAACAGA CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG
    GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGCCTAG AGCGGAGTGA ACGGGGAGGT
    GACCGTGGTG ACCGGCTTGA TCGCGCCCGG ACACCCGCCA CCAAGCGGAG CTTCAGCAAG
    GAAGTGGAGG AACGGAGTAG AGAGCGGCCC TCTCAGCCTG ATGGACTACG CAAGGCAGCT
    AGCCTCACGG AGGATCGGGA CCGCGGGCGG GAAGCTGTGA AACGAGAAGC TGCCCTCCCC
    CCGGTGAGTC CCCCGAAGGC TGCACTCTCT GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC
    ATCATTGAGG AGTACCTCCA TCTCAATGAC ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG
    CTGGCTTCGC CCTCCCTGCT CTTTATCTTT GTACGGCACG GCATCGAGTC CACGCTGGAG
    CGCAGCGCCA TCACGCGGGA GCACATGGGC CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG
    CACCTCTCCA CTGCTCAGTA CTACCAAGGG CTATATGAAA TTCTAGAGTT GGCTGAAGAC
    ATGGAAATTG ACATCCCTCA TGTGTGGCTC TACCTAGCAG AATTGATAAC GCCCATTCTG
    CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG TTCAGGGAAA TAACAAAACC TCTGAGACCC
    CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG ATCCTGGGGC TCCTGTGCAA AAGCATGGGT
    CCTAAAAAGG TGGGGACGCT GTGGCGAGAG GCTGGACTCA GCTGGAAGGA ATTTCTGCCT
    GAAGGCCAGG ATGTCAGTGC CTTTGTCACT GCGCAGAAGG TGGAGTATAC CCTGGGAGAG
    GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG
    AAGCTGCTGC AGGAGGGCAG CAGTAACCAG CGGGTGTTTG ACTGGATAGA GGCCAACCTG
    AACGAGCAGC AGGTGACATC CAACACATTA GTTCGAGCCC TTATGACAAC TGTCTGCTAT
    TCTGCAATTA TCTTTGAGTC TTCTCTCCGA GTGGATGTTG CAGTGCTGAA AGCACGAGCG
    AAACTGCTAC AGAAATACCT GTGTGACGAG CAGAAGGAGC TGCAGGCGCT CTATGCCCTC
    CAGGCCCTTG TAGTGACCTT AGACCAGCCC CCTAACCTGC TTGGGATGTT CTTCGATGCG
    CTGTATGACG AGGACGTGGT GAAGGAGGAC GCCTTCTATA GCTGGGAGAG TAGCAAGGAC
    CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC CTAAAATCTG TCACGACTTT TTTCAAGTGG
    CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851093.1
    CDS248..4966
    Translation

    Target ORF information:

    RefSeq Version XM_024995325.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA
    GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG
    ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC
    CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC
    TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG
    GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC
    CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG
    AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC
    CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC
    GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC
    CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC
    CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA
    ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT
    GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA
    GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT
    CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA
    TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA
    GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC
    AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG
    GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG
    GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA
    GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC
    CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG
    TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC
    CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT
    GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA
    GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA
    GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA
    AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG
    CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG
    CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT
    TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT
    GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC CTGGGACCCC GGCGATCTCA GCAGGGCCCC
    CGAAAGGAAC CACGCAAGAT CATTGCCACA GTGTCAATGA CCGAAGATAT AAAGCTGAAC
    AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC AAGCGGACGG CGGCTGATAA GGACCGAGGA
    GAAGAGGATG CTGATGGCAG CAAAACCCAG GACCTGTTCC GCAGGGTGCG CTCCATCTTG
    AATAAGCTGA CACCCCAGAT GTTCCAGCAG CTGATGAAGC AGGTGACGCA GCTAGCCATT
    GATACCGAGG AACGTCTCAA AGGGGTCATT GACCTCATCT TCGAGAAGGC CATTTCAGAA
    CCCAACTTCT CCGTGGCCTA TGCCAACATG TGCCGCTGCC TCATGGCGCT GAAAGTGCCC
    ACTACAGAAA AGCCAACAGT GACTGTGAAC TTCCGAAAAC TGTTGTTAAA TCGATGTCAG
    AAGGAGTTTG AAAAAGACAA AGATGATGAT GAGGTTTTTG AGAAGAAGCA AAAAGAGATG
    GATGAAGCTG CTACGGCAGA GGAACGGGGA CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA
    GACATAGCCC GGCGGCGCTC GTTAGGGAAT ATCAAGTTTA TCGGGGAGTT GTTCAAATTG
    AAGATGTTAA CAGAGGCAAT CATGCATGAC TGTGTGGTTA AACTACTTAA GAACCATGAT
    GAAGAGTCCC TTGAATGCCT TTGCCGTCTG CTCACCACCA TTGGCAAAGA CCTGGACTTT
    GAGAAGGCCA AGCCCCGGAT GGATCAGTAT TTCAACCAGA TGGAAAAAAT CATTAAGGAA
    AAGAAGACTT CATCCCGGAT CCGCTTTATG CTGCAGGATG TGCTGGATCT GCGAAAGAGC
    AACTGGGTGC CACGCCGTGG GGACCAGGGG CCCAAGACAA TTGACCAAAT CCACAAGGAA
    GCTGAGATGG AGGAGCATCG GGAGCACATA AAAGTGCAGC AGCTAATGGC CAAGGGCAGC
    GACAAGCGTC GGGGTGGCCC TCCAGGCCCA CCCATCAGCC GTGGCCTTCC ACTTGTGGAT
    GATGGTGGCT GGAACACAGT ACCCATCAGC AAGGGCAGCC GCCCTATTGA CACTTCTCGA
    CTCACTAAGA TCACAAAGCC TGGCTCCATT GATTCTAATA ACCAGCTGTT TGCACCTGGA
    GGCCGATTGA GCTGGGGCAA GGGTAGCAGT GGAGGCTCAG GAGCCAAGCC CTCCGATGCA
    GCATCAGAAG CTGCTCGTCC AGCTACTAGT ACCTTGAATC GCTTCTCAGC CCTTCAACAA
    GCAGTACCAA CAGAAAGCAC AGACAACAGA CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG
    GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGCCTAG AGCGGAGTGA ACGGGGAGGT
    GACCGTGGTG ACCGGCTTGA TCGCGCCCGG ACACCCGCCA CCAAGCGGAG CTTCAGCAAG
    GAAGTGGAGG AACGGAGTAG AGAGCGGCCC TCTCAGCCTG ATGGACTACG CAAGGCAGCT
    AGCCTCACGG AGGATCGGGA CCGCGGGCGG GAAGCTGTGA AACGAGAAGC TGCCCTCCCC
    CCGGTGAGTC CCCCGAAGGC TGCACTCTCT GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC
    ATCATTGAGG AGTACCTCCA TCTCAATGAC ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG
    CTGGCTTCGC CCTCCCTGCT CTTTATCTTT GTACGGCACG GCATCGAGTC CACGCTGGAG
    CGCAGCGCCA TCACGCGGGA GCACATGGGC CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG
    CACCTCTCCA CTGCTCAGTA CTACCAAGGG CTATATGAAA TTCTAGAGTT GGCTGAAGAC
    ATGGAAATTG ACATCCCTCA TGTGTGGCTC TACCTAGCAG AATTGATAAC GCCCATTCTG
    CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG TTCAGGGAAA TAACAAAACC TCTGAGACCC
    CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG ATCCTGGGGC TCCTGTGCAA AAGCATGGGT
    CCTAAAAAGG TGGGGACGCT GTGGCGAGAG GCTGGACTCA GCTGGAAGGA ATTTCTGCCT
    GAAGGCCAGG ATGTCAGTGC CTTTGTCACT GCGCAGAAGG TGGAGTATAC CCTGGGAGAG
    GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG
    AAGCTGCTGC AGGAGGGCAG CAGTAACCAG CGGGTGTTTG ACTGGATAGA GGCCAACCTG
    AACGAGCAGC AGGTGACATC CAACACATTA GTTCGAGCCC TTATGACAAC TGTCTGCTAT
    TCTGCAATTA TCTTTGAGTC TTCTCTCCGA GTGGATGTTG CAGTGCTGAA AGCACGAGCG
    AAACTGCTAC AGAAATACCT GTGTGACGAG CAGAAGGAGC TGCAGGCGCT CTATGCCCTC
    CAGGCCCTTG TAGTGACCTT AGACCAGCCC CCTAACCTGC TTGGGATGTT CTTCGATGCG
    CTGTATGACG AGGACGTGGT GAAGGAGGAC GCCTTCTATA GCTGGGAGAG TAGCAAGGAC
    CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC CTAAAATCTG TCACGACTTT TTTCAAGTGG
    CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219176
    Clone ID Related Accession (Same CDS sequence) XM_024995329.1
    Accession Version XM_024995329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA
    GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG
    ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC
    CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC
    TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG
    GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC
    CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG
    AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC
    CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC
    GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC
    CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC
    CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA
    ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT
    GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA
    GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT
    CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA
    TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA
    GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC
    AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG
    GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG
    GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA
    GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC
    CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG
    TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC
    CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT
    GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA
    GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA
    GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA
    AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG
    CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG
    CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT
    TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT
    GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG GGACCCCGGC GATCTCAGCA GGGCCCCCGA
    AAGGAACCAC GCAAGATCAT TGCCACAGTG TCAATGACCG AAGATATAAA GCTGAACAAA
    GCAGAAAAGG CCTGGAAACC CAGTAGCAAG CGGACGGCGG CTGATAAGGA CCGAGGAGAA
    GAGGATGCTG ATGGCAGCAA AACCCAGGAC CTGTTCCGCA GGGTGCGCTC CATCTTGAAT
    AAGCTGACAC CCCAGATGTT CCAGCAGCTG ATGAAGCAGG TGACGCAGCT AGCCATTGAT
    ACCGAGGAAC GTCTCAAAGG GGTCATTGAC CTCATCTTCG AGAAGGCCAT TTCAGAACCC
    AACTTCTCCG TGGCCTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT
    ACAGAAAAGC CAACAGTGAC TGTGAACTTC CGAAAACTGT TGTTAAATCG ATGTCAGAAG
    GAGTTTGAAA AAGACAAAGA TGATGATGAG GTTTTTGAGA AGAAGCAAAA AGAGATGGAT
    GAAGCTGCTA CGGCAGAGGA ACGGGGACGC CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC
    ATAGCCCGGC GGCGCTCGTT AGGGAATATC AAGTTTATCG GGGAGTTGTT CAAATTGAAG
    ATGTTAACAG AGGCAATCAT GCATGACTGT GTGGTTAAAC TACTTAAGAA CCATGATGAA
    GAGTCCCTTG AATGCCTTTG CCGTCTGCTC ACCACCATTG GCAAAGACCT GGACTTTGAG
    AAGGCCAAGC CCCGGATGGA TCAGTATTTC AACCAGATGG AAAAAATCAT TAAGGAAAAG
    AAGACTTCAT CCCGGATCCG CTTTATGCTG CAGGATGTGC TGGATCTGCG AAAGAGCAAC
    TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC AAGACAATTG ACCAAATCCA CAAGGAAGCT
    GAGATGGAGG AGCATCGGGA GCACATAAAA GTGCAGCAGC TAATGGCCAA GGGCAGCGAC
    AAGCGTCGGG GTGGCCCTCC AGGCCCACCC ATCAGCCGTG GCCTTCCACT TGTGGATGAT
    GGTGGCTGGA ACACAGTACC CATCAGCAAG GGCAGCCGCC CTATTGACAC TTCTCGACTC
    ACTAAGATCA CAAAGCCTGG CTCCATTGAT TCTAATAACC AGCTGTTTGC ACCTGGAGGC
    CGATTGAGCT GGGGCAAGGG TAGCAGTGGA GGCTCAGGAG CCAAGCCCTC CGATGCAGCA
    TCAGAAGCTG CTCGTCCAGC TACTAGTACC TTGAATCGCT TCTCAGCCCT TCAACAAGCA
    GTACCAACAG AAAGCACAGA CAACAGACGA GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA
    CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC CGCCTAGAGC GGAGTGAACG GGGAGGTGAC
    CGTGGTGACC GGCTTGATCG CGCCCGGACA CCCGCCACCA AGCGGAGCTT CAGCAAGGAA
    GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT CAGCCTGATG GACTACGCAA GGCAGCTAGC
    CTCACGGAGG ATCGGGACCG CGGGCGGGAA GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG
    GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA GAGGAGCTGG AGAAGAAATC CAGGGCCATC
    ATTGAGGAGT ACCTCCATCT CAATGACATG AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG
    GCTTCGCCCT CCCTGCTCTT TATCTTTGTA CGGCACGGCA TCGAGTCCAC GCTGGAGCGC
    AGCGCCATCA CGCGGGAGCA CATGGGCCGG CTGCTGCACC AGCTGCTCTG TGCTGGGCAC
    CTCTCCACTG CTCAGTACTA CCAAGGGCTA TATGAAATTC TAGAGTTGGC TGAAGACATG
    GAAATTGACA TCCCTCATGT GTGGCTCTAC CTAGCAGAAT TGATAACGCC CATTCTGCAG
    GAAGGTGGGG TGCCAATGGG GGAGCTGTTC AGGGAAATAA CAAAACCTCT GAGACCCCTG
    GGCAAGGCTG CTTCCCTGTT GCTGGAGATC CTGGGGCTCC TGTGCAAAAG CATGGGTCCT
    AAAAAGGTGG GGACGCTGTG GCGAGAGGCT GGACTCAGCT GGAAGGAATT TCTGCCTGAA
    GGCCAGGATG TCAGTGCCTT TGTCACTGCG CAGAAGGTGG AGTATACCCT GGGAGAGGAG
    TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG
    CTGCTGCAGG AGGGCAGCAG TAACCAGCGG GTGTTTGACT GGATAGAGGC CAACCTGAAC
    GAGCAGCAGG TGACATCCAA CACATTAGTT CGAGCCCTTA TGACAACTGT CTGCTATTCT
    GCAATTATCT TTGAGTCTTC TCTCCGAGTG GATGTTGCAG TGCTGAAAGC ACGAGCGAAA
    CTGCTACAGA AATACCTGTG TGACGAGCAG AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG
    GCCCTTGTAG TGACCTTAGA CCAGCCCCCT AACCTGCTTG GGATGTTCTT CGATGCGCTG
    TATGACGAGG ACGTGGTGAA GGAGGACGCC TTCTATAGCT GGGAGAGTAG CAAGGACCCT
    GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA AAATCTGTCA CGACTTTTTT CAAGTGGCTT
    CGTGAGGCGG AGGAGGAGGA GTCTGACCAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851097.1
    CDS229..4944
    Translation

    Target ORF information:

    RefSeq Version XM_024995329.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA
    GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG
    ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC
    CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC
    TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG
    GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC
    CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG
    AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC
    CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC
    GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC
    CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC
    CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA
    ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT
    GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA
    GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT
    CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA
    TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA
    GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC
    AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG
    GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG
    GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA
    GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC
    CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG
    TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC
    CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT
    GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA
    GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA
    GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA
    AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG
    CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG
    CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT
    TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT
    GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG GGACCCCGGC GATCTCAGCA GGGCCCCCGA
    AAGGAACCAC GCAAGATCAT TGCCACAGTG TCAATGACCG AAGATATAAA GCTGAACAAA
    GCAGAAAAGG CCTGGAAACC CAGTAGCAAG CGGACGGCGG CTGATAAGGA CCGAGGAGAA
    GAGGATGCTG ATGGCAGCAA AACCCAGGAC CTGTTCCGCA GGGTGCGCTC CATCTTGAAT
    AAGCTGACAC CCCAGATGTT CCAGCAGCTG ATGAAGCAGG TGACGCAGCT AGCCATTGAT
    ACCGAGGAAC GTCTCAAAGG GGTCATTGAC CTCATCTTCG AGAAGGCCAT TTCAGAACCC
    AACTTCTCCG TGGCCTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT
    ACAGAAAAGC CAACAGTGAC TGTGAACTTC CGAAAACTGT TGTTAAATCG ATGTCAGAAG
    GAGTTTGAAA AAGACAAAGA TGATGATGAG GTTTTTGAGA AGAAGCAAAA AGAGATGGAT
    GAAGCTGCTA CGGCAGAGGA ACGGGGACGC CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC
    ATAGCCCGGC GGCGCTCGTT AGGGAATATC AAGTTTATCG GGGAGTTGTT CAAATTGAAG
    ATGTTAACAG AGGCAATCAT GCATGACTGT GTGGTTAAAC TACTTAAGAA CCATGATGAA
    GAGTCCCTTG AATGCCTTTG CCGTCTGCTC ACCACCATTG GCAAAGACCT GGACTTTGAG
    AAGGCCAAGC CCCGGATGGA TCAGTATTTC AACCAGATGG AAAAAATCAT TAAGGAAAAG
    AAGACTTCAT CCCGGATCCG CTTTATGCTG CAGGATGTGC TGGATCTGCG AAAGAGCAAC
    TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC AAGACAATTG ACCAAATCCA CAAGGAAGCT
    GAGATGGAGG AGCATCGGGA GCACATAAAA GTGCAGCAGC TAATGGCCAA GGGCAGCGAC
    AAGCGTCGGG GTGGCCCTCC AGGCCCACCC ATCAGCCGTG GCCTTCCACT TGTGGATGAT
    GGTGGCTGGA ACACAGTACC CATCAGCAAG GGCAGCCGCC CTATTGACAC TTCTCGACTC
    ACTAAGATCA CAAAGCCTGG CTCCATTGAT TCTAATAACC AGCTGTTTGC ACCTGGAGGC
    CGATTGAGCT GGGGCAAGGG TAGCAGTGGA GGCTCAGGAG CCAAGCCCTC CGATGCAGCA
    TCAGAAGCTG CTCGTCCAGC TACTAGTACC TTGAATCGCT TCTCAGCCCT TCAACAAGCA
    GTACCAACAG AAAGCACAGA CAACAGACGA GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA
    CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC CGCCTAGAGC GGAGTGAACG GGGAGGTGAC
    CGTGGTGACC GGCTTGATCG CGCCCGGACA CCCGCCACCA AGCGGAGCTT CAGCAAGGAA
    GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT CAGCCTGATG GACTACGCAA GGCAGCTAGC
    CTCACGGAGG ATCGGGACCG CGGGCGGGAA GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG
    GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA GAGGAGCTGG AGAAGAAATC CAGGGCCATC
    ATTGAGGAGT ACCTCCATCT CAATGACATG AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG
    GCTTCGCCCT CCCTGCTCTT TATCTTTGTA CGGCACGGCA TCGAGTCCAC GCTGGAGCGC
    AGCGCCATCA CGCGGGAGCA CATGGGCCGG CTGCTGCACC AGCTGCTCTG TGCTGGGCAC
    CTCTCCACTG CTCAGTACTA CCAAGGGCTA TATGAAATTC TAGAGTTGGC TGAAGACATG
    GAAATTGACA TCCCTCATGT GTGGCTCTAC CTAGCAGAAT TGATAACGCC CATTCTGCAG
    GAAGGTGGGG TGCCAATGGG GGAGCTGTTC AGGGAAATAA CAAAACCTCT GAGACCCCTG
    GGCAAGGCTG CTTCCCTGTT GCTGGAGATC CTGGGGCTCC TGTGCAAAAG CATGGGTCCT
    AAAAAGGTGG GGACGCTGTG GCGAGAGGCT GGACTCAGCT GGAAGGAATT TCTGCCTGAA
    GGCCAGGATG TCAGTGCCTT TGTCACTGCG CAGAAGGTGG AGTATACCCT GGGAGAGGAG
    TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG
    CTGCTGCAGG AGGGCAGCAG TAACCAGCGG GTGTTTGACT GGATAGAGGC CAACCTGAAC
    GAGCAGCAGG TGACATCCAA CACATTAGTT CGAGCCCTTA TGACAACTGT CTGCTATTCT
    GCAATTATCT TTGAGTCTTC TCTCCGAGTG GATGTTGCAG TGCTGAAAGC ACGAGCGAAA
    CTGCTACAGA AATACCTGTG TGACGAGCAG AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG
    GCCCTTGTAG TGACCTTAGA CCAGCCCCCT AACCTGCTTG GGATGTTCTT CGATGCGCTG
    TATGACGAGG ACGTGGTGAA GGAGGACGCC TTCTATAGCT GGGAGAGTAG CAAGGACCCT
    GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA AAATCTGTCA CGACTTTTTT CAAGTGGCTT
    CGTGAGGCGG AGGAGGAGGA GTCTGACCAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219177
    Clone ID Related Accession (Same CDS sequence) XM_024995330.1
    Accession Version XM_024995330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGC CGTTCCACAT ATGTTGTCCC GACACAGCAG
    TATCCTGTGC AGCCGGGAGC CCCAAGCTTC TACCCAGGTG CAAGCCCTAC AGAGTTTGGG
    ACCTACGCTG GCGCTTACTA CCCAGCCCAG GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT
    CCCCCACCTG TTTTGATGAA CCAGCCACCC CAGATTGCTC CCAAGAGGGA GCGGAAGACG
    ATCCGAATTC GAGACCCAAA CCAAGGAGGG AAGGATATCA CGGAGGAGAT CATGTCTGGG
    GCCCGCACTG CCTCCACACC CACACCTCCC CAGACGGGAG GCGGTCTGGA GCCTCAGGCT
    AATGGGGAGA CACCCCAGGT TGCTGTCGTC GTCCGGCCAG ATGACCGGTC GCAGGGAGCA
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851098.1
    CDS248..4876
    Translation

    Target ORF information:

    RefSeq Version XM_024995330.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGC CGTTCCACAT ATGTTGTCCC GACACAGCAG
    TATCCTGTGC AGCCGGGAGC CCCAAGCTTC TACCCAGGTG CAAGCCCTAC AGAGTTTGGG
    ACCTACGCTG GCGCTTACTA CCCAGCCCAG GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT
    CCCCCACCTG TTTTGATGAA CCAGCCACCC CAGATTGCTC CCAAGAGGGA GCGGAAGACG
    ATCCGAATTC GAGACCCAAA CCAAGGAGGG AAGGATATCA CGGAGGAGAT CATGTCTGGG
    GCCCGCACTG CCTCCACACC CACACCTCCC CAGACGGGAG GCGGTCTGGA GCCTCAGGCT
    AATGGGGAGA CACCCCAGGT TGCTGTCGTC GTCCGGCCAG ATGACCGGTC GCAGGGAGCA
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219178
    Clone ID Related Accession (Same CDS sequence) XM_024995332.1
    Accession Version XM_024995332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    GGCCGTTCCA CATATGTTGT CCCGACACAG CAGTATCCTG TGCAGCCGGG AGCCCCAAGC
    TTCTACCCAG GTGCAAGCCC TACAGAGTTT GGGACCTACG CTGGCGCTTA CTACCCAGCC
    CAGGGTGTGC AGCAGTTTCC CACTGGTGTA GCTCCCCCAC CTGTTTTGAT GAACCAGCCA
    CCCCAGATTG CTCCCAAGAG GGAGCGGAAG ACGATCCGAA TTCGAGACCC AAACCAAGGA
    GGGAAGGATA TCACGGAGGA GATCATGTCT GGGGCCCGCA CTGCCTCCAC ACCCACACCT
    CCCCAGACGG GAGGCGGTCT GGAGCCTCAG GCTAATGGGG AGACACCCCA GGTTGCTGTC
    GTCGTCCGGC CAGATGACCG GTCGCAGGGA GCAATCATTG GGGAGCGGCC AGGGCTGCCT
    GGCCCAGAGC ACAGCCCTTC AGAATCCCAG CCTTCATCAC CTTCTCCAAC CCCATCACCA
    CCCCCAGTCT TGGAACCGGG GTCTGAGCCT AATCTCGCAG TCCTCTCTGT TCCTGGGGAC
    ACAATGACAA CAGGGATGAT ACAGACGTCT GTAGAAGAAT CAACCCCCAC GCCCCCTGAA
    AGTGGGGAGC CGTGTTGCCT CTCTCCAGAA CCCACTCCCC TTGCTGAACC CATATTGGAA
    GTAGAAGTGA CGCTGAGCAA ACCAGTTCCA GAATCTGAGT TCTCTTCCAG TCCTCTCCAG
    GTTCCCAGCC CCCTGGCATC TCACAAGGTG GAAATTCTTC CTGAGCCTAA TGGCACAGTC
    CCATCTGAGA ATTTGGAACC AGAGGTGGAG TCAAGCCCAG AGCTTGCCCC TCTCCCTCCT
    CCAGCTTGTC CCTCTGAATC CCCCATGCCT ATTGCTCCAA CTGCCCAACC TGAGGAACTA
    CTCAACGGAG CCCCTTCACC ACCAGCTGTG GACTTAAGCC CAGTCAGTGA ACCAGAGGAA
    CAGGCCAAGG AGGCTATAGT GTCGGTGACT CCCCCCACTG TCCTTTCTGC TACCCCAGCT
    GTGGCTCCTC CAGTGGCTTC CCCTGCTCAG GAGGAGGACA TGGAGGAGGA GGAAGAAGAG
    GAAGAGGAAG GAGAAGCTGA GGGTGAGAAG GGAGGAGAGG AACCTCTCCC CCAAGAGAGC
    ACCCCTGTCC CAGCCCACCT ATCCCAGAAT TCGGAGGTGG CAGTAGCCAC CCAAGTGGCA
    GTGTCTGTGC CAAAGAGGAG ACGGAAAATT AAGGAGCTCA ATAAGAAGGA GGCTGTAGGA
    GACCTTCTAG ATGCCTTCAA GGAGGCGAAC CCAGGGGTAC CAGAGGTGGA AAATCAGCCT
    CCTGCAGGCT GCAATCCCAG CCCAGAGCCT GAGGGCAGCA GTGTGCCCCT GCGGTCTGAG
    GAAGGAGAGG AGACCTGGGA CTCAAAGGAA GACAAGATTC AAAATGCAGA GAATATCCAG
    CCAGGGGAAC AGAAGTATGA ATATAAGTCA GATCAGTGGA AGCCTCTAAA CCTTGAGGAG
    AAAAAGCGTT ATGACCGAGA GTTCCTGCTT GGCTTTCAGT TCATCTTTGC CAGTATGCAG
    AAGCCTGAGG GATTGCCCCA CATCAGTGAT GTGGTGTTGG ATAAGGCCAA TAAAACACCA
    TTGCGACCGC TGGACCCCTC TAGACTTCAG GGCATAAATT GTGGCCCAGA CTTCACTCCG
    TCTTTTGCCA ACCTTGGCCG ACCAGCCCTT AGCAACCGTG GGCCCCCGAG GGGTGGGCCA
    GGTGGGGAGC TGCCCCGAGG GCCGCAGGCT GGCCTGGGAC CCCGGCGATC TCAGCAGGGC
    CCCCGAAAGG AACCACGCAA GATCATTGCC ACAGTGTCAA TGACCGAAGA TATAAAGCTG
    AACAAAGCAG AAAAGGCCTG GAAACCCAGT AGCAAGCGGA CGGCGGCTGA TAAGGACCGA
    GGAGAAGAGG ATGCTGATGG CAGCAAAACC CAGGACCTGT TCCGCAGGGT GCGCTCCATC
    TTGAATAAGC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAGGTGAC GCAGCTAGCC
    ATTGATACCG AGGAACGTCT CAAAGGGGTC ATTGACCTCA TCTTCGAGAA GGCCATTTCA
    GAACCCAACT TCTCCGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG
    CCCACTACAG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AACTGTTGTT AAATCGATGT
    CAGAAGGAGT TTGAAAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG
    ATGGATGAAG CTGCTACGGC AGAGGAACGG GGACGCCTGA AAGAAGAGCT GGAAGAGGCT
    CGAGACATAG CCCGGCGGCG CTCGTTAGGG AATATCAAGT TTATCGGGGA GTTGTTCAAA
    TTGAAGATGT TAACAGAGGC AATCATGCAT GACTGTGTGG TTAAACTACT TAAGAACCAT
    GATGAAGAGT CCCTTGAATG CCTTTGCCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC
    TTTGAGAAGG CCAAGCCCCG GATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAG
    GAAAAGAAGA CTTCATCCCG GATCCGCTTT ATGCTGCAGG ATGTGCTGGA TCTGCGAAAG
    AGCAACTGGG TGCCACGCCG TGGGGACCAG GGGCCCAAGA CAATTGACCA AATCCACAAG
    GAAGCTGAGA TGGAGGAGCA TCGGGAGCAC ATAAAAGTGC AGCAGCTAAT GGCCAAGGGC
    AGCGACAAGC GTCGGGGTGG CCCTCCAGGC CCACCCATCA GCCGTGGCCT TCCACTTGTG
    GATGATGGTG GCTGGAACAC AGTACCCATC AGCAAGGGCA GCCGCCCTAT TGACACTTCT
    CGACTCACTA AGATCACAAA GCCTGGCTCC ATTGATTCTA ATAACCAGCT GTTTGCACCT
    GGAGGCCGAT TGAGCTGGGG CAAGGGTAGC AGTGGAGGCT CAGGAGCCAA GCCCTCCGAT
    GCAGCATCAG AAGCTGCTCG TCCAGCTACT AGTACCTTGA ATCGCTTCTC AGCCCTTCAA
    CAAGCAGTAC CAACAGAAAG CACAGACAAC AGACGAGTGG TGCAGAGGAG TAGCTTGAGC
    CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGCC TAGAGCGGAG TGAACGGGGA
    GGTGACCGTG GTGACCGGCT TGATCGCGCC CGGACACCCG CCACCAAGCG GAGCTTCAGC
    AAGGAAGTGG AGGAACGGAG TAGAGAGCGG CCCTCTCAGC CTGATGGACT ACGCAAGGCA
    GCTAGCCTCA CGGAGGATCG GGACCGCGGG CGGGAAGCTG TGAAACGAGA AGCTGCCCTC
    CCCCCGGTGA GTCCCCCGAA GGCTGCACTC TCTGAAGAGG AGCTGGAGAA GAAATCCAGG
    GCCATCATTG AGGAGTACCT CCATCTCAAT GACATGAAGG AGGCAGTGCA GTGCGTGCAG
    GAGCTGGCTT CGCCCTCCCT GCTCTTTATC TTTGTACGGC ACGGCATCGA GTCCACGCTG
    GAGCGCAGCG CCATCACGCG GGAGCACATG GGCCGGCTGC TGCACCAGCT GCTCTGTGCT
    GGGCACCTCT CCACTGCTCA GTACTACCAA GGGCTATATG AAATTCTAGA GTTGGCTGAA
    GACATGGAAA TTGACATCCC TCATGTGTGG CTCTACCTAG CAGAATTGAT AACGCCCATT
    CTGCAGGAAG GTGGGGTGCC AATGGGGGAG CTGTTCAGGG AAATAACAAA ACCTCTGAGA
    CCCCTGGGCA AGGCTGCTTC CCTGTTGCTG GAGATCCTGG GGCTCCTGTG CAAAAGCATG
    GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAGGCTGGAC TCAGCTGGAA GGAATTTCTG
    CCTGAAGGCC AGGATGTCAG TGCCTTTGTC ACTGCGCAGA AGGTGGAGTA TACCCTGGGA
    GAGGAGTTAG AAGCCCCAGG CCAGAGGTTG CTGTCCTTTG AGGAGCTGCG CAGGCAGCTG
    GAGAAGCTGC TGCAGGAGGG CAGCAGTAAC CAGCGGGTGT TTGACTGGAT AGAGGCCAAC
    CTGAACGAGC AGCAGGTGAC ATCCAACACA TTAGTTCGAG CCCTTATGAC AACTGTCTGC
    TATTCTGCAA TTATCTTTGA GTCTTCTCTC CGAGTGGATG TTGCAGTGCT GAAAGCACGA
    GCGAAACTGC TACAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTGCAGGC GCTCTATGCC
    CTCCAGGCCC TTGTAGTGAC CTTAGACCAG CCCCCTAACC TGCTTGGGAT GTTCTTCGAT
    GCGCTGTATG ACGAGGACGT GGTGAAGGAG GACGCCTTCT ATAGCTGGGA GAGTAGCAAG
    GACCCTGCTG AGCAGCAGGG CAAGGGCGTG GCCCTAAAAT CTGTCACGAC TTTTTTCAAG
    TGGCTTCGTG AGGCGGAGGA GGAGGAGTCT GACCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851100.1
    CDS248..4789
    Translation

    Target ORF information:

    RefSeq Version XM_024995332.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    GGCCGTTCCA CATATGTTGT CCCGACACAG CAGTATCCTG TGCAGCCGGG AGCCCCAAGC
    TTCTACCCAG GTGCAAGCCC TACAGAGTTT GGGACCTACG CTGGCGCTTA CTACCCAGCC
    CAGGGTGTGC AGCAGTTTCC CACTGGTGTA GCTCCCCCAC CTGTTTTGAT GAACCAGCCA
    CCCCAGATTG CTCCCAAGAG GGAGCGGAAG ACGATCCGAA TTCGAGACCC AAACCAAGGA
    GGGAAGGATA TCACGGAGGA GATCATGTCT GGGGCCCGCA CTGCCTCCAC ACCCACACCT
    CCCCAGACGG GAGGCGGTCT GGAGCCTCAG GCTAATGGGG AGACACCCCA GGTTGCTGTC
    GTCGTCCGGC CAGATGACCG GTCGCAGGGA GCAATCATTG GGGAGCGGCC AGGGCTGCCT
    GGCCCAGAGC ACAGCCCTTC AGAATCCCAG CCTTCATCAC CTTCTCCAAC CCCATCACCA
    CCCCCAGTCT TGGAACCGGG GTCTGAGCCT AATCTCGCAG TCCTCTCTGT TCCTGGGGAC
    ACAATGACAA CAGGGATGAT ACAGACGTCT GTAGAAGAAT CAACCCCCAC GCCCCCTGAA
    AGTGGGGAGC CGTGTTGCCT CTCTCCAGAA CCCACTCCCC TTGCTGAACC CATATTGGAA
    GTAGAAGTGA CGCTGAGCAA ACCAGTTCCA GAATCTGAGT TCTCTTCCAG TCCTCTCCAG
    GTTCCCAGCC CCCTGGCATC TCACAAGGTG GAAATTCTTC CTGAGCCTAA TGGCACAGTC
    CCATCTGAGA ATTTGGAACC AGAGGTGGAG TCAAGCCCAG AGCTTGCCCC TCTCCCTCCT
    CCAGCTTGTC CCTCTGAATC CCCCATGCCT ATTGCTCCAA CTGCCCAACC TGAGGAACTA
    CTCAACGGAG CCCCTTCACC ACCAGCTGTG GACTTAAGCC CAGTCAGTGA ACCAGAGGAA
    CAGGCCAAGG AGGCTATAGT GTCGGTGACT CCCCCCACTG TCCTTTCTGC TACCCCAGCT
    GTGGCTCCTC CAGTGGCTTC CCCTGCTCAG GAGGAGGACA TGGAGGAGGA GGAAGAAGAG
    GAAGAGGAAG GAGAAGCTGA GGGTGAGAAG GGAGGAGAGG AACCTCTCCC CCAAGAGAGC
    ACCCCTGTCC CAGCCCACCT ATCCCAGAAT TCGGAGGTGG CAGTAGCCAC CCAAGTGGCA
    GTGTCTGTGC CAAAGAGGAG ACGGAAAATT AAGGAGCTCA ATAAGAAGGA GGCTGTAGGA
    GACCTTCTAG ATGCCTTCAA GGAGGCGAAC CCAGGGGTAC CAGAGGTGGA AAATCAGCCT
    CCTGCAGGCT GCAATCCCAG CCCAGAGCCT GAGGGCAGCA GTGTGCCCCT GCGGTCTGAG
    GAAGGAGAGG AGACCTGGGA CTCAAAGGAA GACAAGATTC AAAATGCAGA GAATATCCAG
    CCAGGGGAAC AGAAGTATGA ATATAAGTCA GATCAGTGGA AGCCTCTAAA CCTTGAGGAG
    AAAAAGCGTT ATGACCGAGA GTTCCTGCTT GGCTTTCAGT TCATCTTTGC CAGTATGCAG
    AAGCCTGAGG GATTGCCCCA CATCAGTGAT GTGGTGTTGG ATAAGGCCAA TAAAACACCA
    TTGCGACCGC TGGACCCCTC TAGACTTCAG GGCATAAATT GTGGCCCAGA CTTCACTCCG
    TCTTTTGCCA ACCTTGGCCG ACCAGCCCTT AGCAACCGTG GGCCCCCGAG GGGTGGGCCA
    GGTGGGGAGC TGCCCCGAGG GCCGCAGGCT GGCCTGGGAC CCCGGCGATC TCAGCAGGGC
    CCCCGAAAGG AACCACGCAA GATCATTGCC ACAGTGTCAA TGACCGAAGA TATAAAGCTG
    AACAAAGCAG AAAAGGCCTG GAAACCCAGT AGCAAGCGGA CGGCGGCTGA TAAGGACCGA
    GGAGAAGAGG ATGCTGATGG CAGCAAAACC CAGGACCTGT TCCGCAGGGT GCGCTCCATC
    TTGAATAAGC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAGGTGAC GCAGCTAGCC
    ATTGATACCG AGGAACGTCT CAAAGGGGTC ATTGACCTCA TCTTCGAGAA GGCCATTTCA
    GAACCCAACT TCTCCGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG
    CCCACTACAG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AACTGTTGTT AAATCGATGT
    CAGAAGGAGT TTGAAAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG
    ATGGATGAAG CTGCTACGGC AGAGGAACGG GGACGCCTGA AAGAAGAGCT GGAAGAGGCT
    CGAGACATAG CCCGGCGGCG CTCGTTAGGG AATATCAAGT TTATCGGGGA GTTGTTCAAA
    TTGAAGATGT TAACAGAGGC AATCATGCAT GACTGTGTGG TTAAACTACT TAAGAACCAT
    GATGAAGAGT CCCTTGAATG CCTTTGCCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC
    TTTGAGAAGG CCAAGCCCCG GATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAG
    GAAAAGAAGA CTTCATCCCG GATCCGCTTT ATGCTGCAGG ATGTGCTGGA TCTGCGAAAG
    AGCAACTGGG TGCCACGCCG TGGGGACCAG GGGCCCAAGA CAATTGACCA AATCCACAAG
    GAAGCTGAGA TGGAGGAGCA TCGGGAGCAC ATAAAAGTGC AGCAGCTAAT GGCCAAGGGC
    AGCGACAAGC GTCGGGGTGG CCCTCCAGGC CCACCCATCA GCCGTGGCCT TCCACTTGTG
    GATGATGGTG GCTGGAACAC AGTACCCATC AGCAAGGGCA GCCGCCCTAT TGACACTTCT
    CGACTCACTA AGATCACAAA GCCTGGCTCC ATTGATTCTA ATAACCAGCT GTTTGCACCT
    GGAGGCCGAT TGAGCTGGGG CAAGGGTAGC AGTGGAGGCT CAGGAGCCAA GCCCTCCGAT
    GCAGCATCAG AAGCTGCTCG TCCAGCTACT AGTACCTTGA ATCGCTTCTC AGCCCTTCAA
    CAAGCAGTAC CAACAGAAAG CACAGACAAC AGACGAGTGG TGCAGAGGAG TAGCTTGAGC
    CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGCC TAGAGCGGAG TGAACGGGGA
    GGTGACCGTG GTGACCGGCT TGATCGCGCC CGGACACCCG CCACCAAGCG GAGCTTCAGC
    AAGGAAGTGG AGGAACGGAG TAGAGAGCGG CCCTCTCAGC CTGATGGACT ACGCAAGGCA
    GCTAGCCTCA CGGAGGATCG GGACCGCGGG CGGGAAGCTG TGAAACGAGA AGCTGCCCTC
    CCCCCGGTGA GTCCCCCGAA GGCTGCACTC TCTGAAGAGG AGCTGGAGAA GAAATCCAGG
    GCCATCATTG AGGAGTACCT CCATCTCAAT GACATGAAGG AGGCAGTGCA GTGCGTGCAG
    GAGCTGGCTT CGCCCTCCCT GCTCTTTATC TTTGTACGGC ACGGCATCGA GTCCACGCTG
    GAGCGCAGCG CCATCACGCG GGAGCACATG GGCCGGCTGC TGCACCAGCT GCTCTGTGCT
    GGGCACCTCT CCACTGCTCA GTACTACCAA GGGCTATATG AAATTCTAGA GTTGGCTGAA
    GACATGGAAA TTGACATCCC TCATGTGTGG CTCTACCTAG CAGAATTGAT AACGCCCATT
    CTGCAGGAAG GTGGGGTGCC AATGGGGGAG CTGTTCAGGG AAATAACAAA ACCTCTGAGA
    CCCCTGGGCA AGGCTGCTTC CCTGTTGCTG GAGATCCTGG GGCTCCTGTG CAAAAGCATG
    GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAGGCTGGAC TCAGCTGGAA GGAATTTCTG
    CCTGAAGGCC AGGATGTCAG TGCCTTTGTC ACTGCGCAGA AGGTGGAGTA TACCCTGGGA
    GAGGAGTTAG AAGCCCCAGG CCAGAGGTTG CTGTCCTTTG AGGAGCTGCG CAGGCAGCTG
    GAGAAGCTGC TGCAGGAGGG CAGCAGTAAC CAGCGGGTGT TTGACTGGAT AGAGGCCAAC
    CTGAACGAGC AGCAGGTGAC ATCCAACACA TTAGTTCGAG CCCTTATGAC AACTGTCTGC
    TATTCTGCAA TTATCTTTGA GTCTTCTCTC CGAGTGGATG TTGCAGTGCT GAAAGCACGA
    GCGAAACTGC TACAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTGCAGGC GCTCTATGCC
    CTCCAGGCCC TTGTAGTGAC CTTAGACCAG CCCCCTAACC TGCTTGGGAT GTTCTTCGAT
    GCGCTGTATG ACGAGGACGT GGTGAAGGAG GACGCCTTCT ATAGCTGGGA GAGTAGCAAG
    GACCCTGCTG AGCAGCAGGG CAAGGGCGTG GCCCTAAAAT CTGTCACGAC TTTTTTCAAG
    TGGCTTCGTG AGGCGGAGGA GGAGGAGTCT GACCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45811
    Clone ID Related Accession (Same CDS sequence) XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
    Accession Version XM_010801318.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799620.1
    CDS637..4785
    Translation

    Target ORF information:

    RefSeq Version XM_010801318.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45811
    Clone ID Related Accession (Same CDS sequence) XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
    Accession Version XM_015462063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317549.1
    CDS876..5024
    Translation

    Target ORF information:

    RefSeq Version XM_015462063.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45811
    Clone ID Related Accession (Same CDS sequence) XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
    Accession Version XM_015462064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317550.1
    CDS637..4785
    Translation

    Target ORF information:

    RefSeq Version XM_015462064.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45811
    Clone ID Related Accession (Same CDS sequence) XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
    Accession Version XM_015472316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327802.1
    CDS876..5024
    Translation

    Target ORF information:

    RefSeq Version XM_015472316.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC
    CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA
    GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC
    AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG
    GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG
    CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT
    GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG
    TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC
    TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT
    GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA
    CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT
    ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC
    TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG
    CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT
    TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG
    CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG
    CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA
    AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA
    CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC
    AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT
    GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT
    GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT
    ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA
    CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG
    ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC
    TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG
    GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT
    GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC
    ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT
    GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC
    CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG
    CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC
    TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG
    TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG
    ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG
    GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT
    GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG
    TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG
    CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA
    GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA
    GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG
    CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC
    CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC
    GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC
    CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC
    CACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45812
    Clone ID Related Accession (Same CDS sequence) XM_010801319.2 , XM_015462065.1
    Accession Version XM_010801319.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG
    GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG
    TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG
    CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC
    CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC
    CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC
    CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC
    AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT
    GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG
    CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC
    AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA
    GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC
    ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG
    GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT
    TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA
    GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC
    TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA
    GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA
    CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT
    CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA
    GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA
    GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG
    AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA
    CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA
    TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC
    CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC
    AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT
    GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC
    TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG
    GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT
    CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG
    GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT
    AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC
    TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA
    AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799621.1
    CDS876..5021
    Translation

    Target ORF information:

    RefSeq Version XM_010801319.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801319.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG
    GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG
    TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG
    CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC
    CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC
    CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC
    CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC
    AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT
    GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG
    CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC
    AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA
    GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC
    ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG
    GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT
    TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA
    GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC
    TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA
    GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA
    CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT
    CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA
    GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA
    GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG
    AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA
    CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA
    TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC
    CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC
    AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT
    GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC
    TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG
    GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT
    CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG
    GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT
    AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC
    TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA
    AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45812
    Clone ID Related Accession (Same CDS sequence) XM_010801319.2 , XM_015462065.1
    Accession Version XM_015462065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG
    GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG
    TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG
    CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC
    CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC
    CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC
    CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC
    AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT
    GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG
    CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC
    AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA
    GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC
    ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG
    GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT
    TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA
    GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC
    TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA
    GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA
    CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT
    CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA
    GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA
    GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG
    AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA
    CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA
    TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC
    CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC
    AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT
    GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC
    TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG
    GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT
    CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG
    GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT
    AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC
    TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA
    AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317551.1
    CDS876..5021
    Translation

    Target ORF information:

    RefSeq Version XM_015462065.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
    ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT
    TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT
    CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA
    GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC
    ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA
    TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA
    ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA
    AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT
    GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC
    TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC
    CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG
    GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA
    GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG
    GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG
    GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA
    GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG
    GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC
    AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT
    CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC
    TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG
    GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC
    ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC
    AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG
    GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG
    TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG
    CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC
    CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG
    ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC
    CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC
    CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG
    GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC
    CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC
    AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT
    GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC
    ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC
    AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG
    CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC
    AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA
    GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC
    ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG
    GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT
    TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA
    GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC
    TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA
    GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC
    CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA
    CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT
    CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA
    GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA
    GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG
    AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA
    CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG
    CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA
    TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC
    CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC
    AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC
    CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT
    GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG
    CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC
    TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG
    GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT
    CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG
    GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG
    AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT
    AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC
    TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA
    AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45813
    Clone ID Related Accession (Same CDS sequence) XM_015462066.1 , XM_010801320.1
    Accession Version XM_015462066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC
    AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC
    TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT
    GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT
    CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG
    CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG
    GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA
    TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC
    CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC
    CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC
    CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG
    CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT
    GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC
    ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA
    AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG
    GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT
    CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA
    AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA
    GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG
    CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG
    AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG
    AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC
    CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC
    CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG
    CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT
    CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC
    CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG
    CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC
    AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA
    GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG
    ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT
    GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC
    GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG
    GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG
    GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317552.1
    CDS35..4858
    Translation

    Target ORF information:

    RefSeq Version XM_015462066.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC
    AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC
    TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT
    GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT
    CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG
    CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG
    GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA
    TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC
    CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC
    CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC
    CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG
    CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT
    GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC
    ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA
    AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG
    GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT
    CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA
    AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA
    GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG
    CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG
    AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG
    AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC
    CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC
    CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG
    CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT
    CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC
    CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG
    CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC
    AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA
    GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG
    ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT
    GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC
    GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG
    GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG
    GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45813
    Clone ID Related Accession (Same CDS sequence) XM_015462066.1 , XM_010801320.1
    Accession Version XM_010801320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC
    AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC
    TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT
    GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT
    CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG
    CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG
    GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA
    TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC
    CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC
    CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC
    CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG
    CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT
    GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC
    ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA
    AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG
    GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT
    CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA
    AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA
    GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG
    CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG
    AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG
    AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC
    CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC
    CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG
    CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT
    CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC
    CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG
    CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC
    AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA
    GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG
    ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT
    GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC
    GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG
    GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG
    GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799622.1
    CDS35..4858
    Translation

    Target ORF information:

    RefSeq Version XM_010801320.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC
    CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT
    GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA
    AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG
    TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC
    AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG
    GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC
    GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT
    GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC
    CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA
    CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG
    ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT
    TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG
    AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG
    GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG
    GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT
    GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT
    TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT
    ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG
    GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA
    GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC
    CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT
    CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC
    TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG
    TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC
    CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG
    CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC
    CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT
    GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC
    AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC
    TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT
    GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT
    CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG
    GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA
    GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG
    CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG
    GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA
    TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC
    CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC
    CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC
    CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG
    CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT
    GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC
    ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA
    AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG
    GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT
    CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA
    AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA
    GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG
    CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG
    AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT
    CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG
    AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC
    CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC
    CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG
    CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT
    CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC
    CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG
    CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT
    TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG
    ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC
    AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA
    GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG
    GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG
    ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT
    GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA
    TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG
    ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC
    GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG
    GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG
    GAGGAGGAGT CTGACCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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