RPTOR cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPTOR
Entrez Gene ID	507056
Full Name	regulatory associated protein of MTOR complex 1
General protein information	Preferred Names regulatory associated protein of MTOR complex 1 Names regulatory-associated protein of mTOR
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221019.3	XP_005221076.1	regulatory-associated protein of mTOR isoform X1
NM_001192130.1	NP_001179059.1	regulatory-associated protein of mTOR
NM_001192130.2	NP_001179059.2	regulatory-associated protein of mTOR
NM_001192130.3	NP_001179059.3	regulatory-associated protein of mTOR
XM_015458921.1	XP_015314407.1	regulatory-associated protein of mTOR isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rptor	NP_083174.2
Rattus norvegicus (Norway rat)	Rptor	NP_001127971.2
Homo sapiens (human)	RPTOR	NP_065812.1
Canis lupus familiaris (dog)	RPTOR	XP_850487.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010035	XP_001689178.1
Kluyveromyces lactis	KLLA0A09471g	XP_451414.1
Magnaporthe oryzae (rice blast fungus)	MGG_02874	XP_003720858.1
Gallus gallus (chicken)	RPTOR	XP_426232.2
Drosophila melanogaster (fruit fly)	raptor	NP_572294.2
Saccharomyces cerevisiae (baker's yeast)	KOG1	NP_012056.1
Schizosaccharomyces pombe (fission yeast)	mip1	NP_593370.1
	RPTOR	XP_001110471.1
Neurospora crassa	NCU00621	XP_965761.1
Arabidopsis thaliana (thale cress)	RAPTOR2	NP_195797.3
Pan troglodytes (chimpanzee)	RPTOR	XP_003953266.1
Bos taurus (cattle)	RPTOR	NP_001179059.1
Danio rerio (zebrafish)	rptor	XP_005157411.1
Eremothecium gossypii	AGOS_ADR331C	NP_984427.2
Arabidopsis thaliana (thale cress)	RAPTOR1	NP_566335.1
Oryza sativa (rice)	Os12g0110000	NP_001065961.1
Xenopus tropicalis (tropical clawed frog)	rptor	NP_001123843.1

Gene Ontology
Bibliography

Related articles in PubMed

Energy depletion of bovine mammary epithelial cells activates AMPK and suppresses protein synthesis through inhibition of mTORC1 signaling.
Burgos SA, Kim JJ, Dai M, Cant JP
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(3)183-9(2013 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that activation of AMPK represses global protein synthesis in mammary epithelial cells through inhibition of mTORC1 signaling.
Title: Energy depletion of bovine mammary epithelial cells activates AMPK and suppresses protein synthesis through inhibition of mTORC1 signaling.

The following RPTOR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPTOR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa216192	NM_001192130.2 Latest version!	Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa239162	NM_001192130.3 Latest version!	Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa94004	XM_015458921.1 Latest version!	Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa40069	NM_001192130.1 Latest version!	Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa30234	XM_005221019.3 Latest version!	Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPTOR ( NM_001192130.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179059.2 Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.

Price & Availability↑

CloneID	OBa216192
Clone ID Related Accession (Same CDS sequence)	NM_001192130.2
Accession Version	NM_001192130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	regulatory-associated protein of mTOR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02049448.1, DAAA02049444.1, DAAA02049442.1, DAAA02049441.1 and DAAA02049440.1. On Jun 30, 2017 this sequence version replaced NM_001192130.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC 
TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC 
ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC 
ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC 
GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG 
AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC 
CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC 
ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC 
AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG 
GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC 
AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179059.2
CDS	758..4765
Translation	MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEANELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLCSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPVVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPATTEGGSLTPVRDSPCTPRLRSVSSYGNIRAVTTARNLNKSLQNLSLTEETGGSVAFSPGNLSTSSSASSTLGSPENEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPDVSDLAMKVLNSIAYKATVNARPQRILSTSSLTQSAPASPTNKGIHIHQAGGSPPTSSASSSSLTNDVAKQPVSRDLPPGRPGATGPVGVQYTPHSHQFPRTRKMFDKGPDQTTDDPDDTAGHKSFISATMQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNTRVRKQAQQLIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCVAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMSSGDVRIIRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSECRVMTYREHTAWVVKAYLQKHPEGHIMSVSVNGDVRFFDPRLPESVNVMQIVKGLTALDIHPQANLIACGSMNQFTAIYNGSGELINHIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Target ORF information:

RefSeq Version	NM_001192130.2
Organism	Bos taurus(cattle)
Definition	Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192130.2

ORF Insert Sequence:

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC 
TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC 
ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC 
ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC 
GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG 
AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC 
CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC 
ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC 
AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG 
GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC 
AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPTOR ( NM_001192130.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179059.3 Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.

Price & Availability↑

CloneID	OBa239162
Clone ID Related Accession (Same CDS sequence)	NM_001192130.3
Accession Version	NM_001192130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-20
Organism	Bos taurus(cattle)
Product	regulatory-associated protein of mTOR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1 and DV879279.1. On Jun 21, 2018 this sequence version replaced NM_001192130.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571261.9519.1, SRR5571260.47242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATT CTCACCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACCGACGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC 
TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC 
ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC 
ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC 
GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG 
AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC 
CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC 
ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC 
AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG 
GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC 
AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179059.3
CDS	758..4765
Translation	MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEANELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLCSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPVVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPATTEGGSLTPVRDSPCTPRLRSVSSYGNIRAVTTARNLNKSLQNLSLTEETGGSVAFSPGNLSTSSSASSTLGSPENEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPDVSDLAMKVLNSIAYKATVNARPQRILTTSSLTQSAPASPTNKGIHIHQAGGSPPTSSASSSSLTNDVAKQPVSRDLPPGRPGTTGPVGVQYTPHSHQFPRTRKMFDKGPDQTTDDPDDTAGHKSFISATMQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNTRVRKQAQQLIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCVAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMSSGDVRIIRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSECRVMTYREHTAWVVKAYLQKHPEGHIMSVSVNGDVRFFDPRLPESVNVMQIVKGLTALDIHPQANLIACGSMNQFTAIYNGSGELINHIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Target ORF information:

RefSeq Version	NM_001192130.3
Organism	Bos taurus(cattle)
Definition	Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192130.3

ORF Insert Sequence:

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATT CTCACCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACCGACGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC 
TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC 
ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC 
ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC 
GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG 
AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC 
CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC 
ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC 
AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG 
GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC 
AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPTOR ( XM_015458921.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314407.1 Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X2, mRNA.

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CloneID	OBa94004
Clone ID Related Accession (Same CDS sequence)	XM_015458921.1
Accession Version	XM_015458921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulatory-associated protein of mTOR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104498.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGC CACTACCGGA TCCTCTGTCG ATGGGTCCTC AGAAAGCTCT GGAAACCATT 
GGTGCAAATC TACAGAAGCA GTACGAGAAC TGGCAGCCAA GGGCGCGGTA TAAGCAGAGC 
CTTGATCCGA CTGTGGATGA AGTAAAGAAG CTTTGCACGT CTTTACGCCG AAATGCCAAG 
GAGGAGCGGG TCCTCTTCCA CTACAATGGC CATGGGGTGC CCAGGCCCAC CGTTAATGGC 
GAGATCTGGG TCTTCAACAA GAACTACACT CAGTACATCC CTCTGTCTAT ATACGACCTG 
CAGACGTGGA TGGGTAGCCC CTCCATATTC GTCTATGACT GCTCCAATGC TGGCCTCATT 
GTCAAGTCCT TCAAGCAGTT CGCCCTGCAG AGGGAGCAGG AGCTGGAGGT CGCTGCTATT 
AACCCAAACC ACCCACTGGC CCAGATGCCT TTGCCCCCGT CTATGAAGAA CTGCATTCAG 
CTGGCAGCCT GTGAGGCCAA CGAGCTCCTG CCCATGATCC CCGACCTCCC AGCTGACCTG 
TTCACATCAT GCCTCACCAC CCCCATCAAA ATCGCCCTGC GCTGGTTCTG CATGCAGAAA 
TGTGTTAGTC TGGTGCCTGG AGTCACACTG GATTTGATAG AAAAGATTCC CGGGCGGCTG 
AATGACAGGA GGACCCCCCT GGGCGAGCTG AACTGGATCT TCACAGCCAT CACGGACACG 
ATTGCGTGGA ACGTGCTTCC TCGGGATCTC TTCCAAAAGC TCTTCAGACA GGACCTGTTG 
GTGGCTAGTC TGTTTCGAAA TTTTTTATTG GCAGAAAGAA TCATGAGGTC ATATAACTGC 
ACCCCCGTCA GCAGTCCACG CCTACCCCCC ACGTACATGC ACGCCATGTG GCAAGCCTGG 
GACCTGGCCG TGGACATCTG TCTCTCCCAG CTGCCGACCA TCATCGAAGA AGGCACAGCA 
TTTCGGCACA GCCCCTTCTT TGCTGAGCAG CTGACCGCCT TCCAGGTGTG GCTCACCATG 
GGCGTGGAAA ACAGGAACCC ACCTGAGCAG CTTCCTATTG TCTTACAGGT GCTGCTGAGC 
CAAGTGCACC GGCTGAGAGC CCTGGACTTG CTGGGAAGAT TTCTGGACCT GGGCCCCTGG 
GCAGTGAGCC TGGCGCTGTC TGTGGGCATC TTCCCCTATG TGCTAAAGCT GCTCCAGAGC 
TCAGCCAGGG AGCTGCGGCC TCTCCTTGTC TTCATCTGGG CCAAGATCCT TGCCGTGGAC 
AGTTCATGCC AAGCTGACCT GGTGAAGGAC AACGGCCACA AGTACTTCCT CTCGGTCTTG 
GCAGACCCGT ACATGCCAGC TGAGCACCGG ACCATGACGG CCTTCATTCT GGCTGTAATC 
GTCAACAGCT ATAACACGGG GCAGGAAGCC TGCCTCCAGG GAAACCTGAT CGCCATCTGC 
CTGGAGCAGC TCAACGACCC CCACCCCCTG CTGCGCCAGT GGGTGGCCAT CTGCCTGGGC 
CGCATCTGGC AGAACTTTGA CTCGGCCCGG TGGTGCGGGG TGCGGGACAG CGCCCACGAG 
AAGCTCTGCA GCCTCCTCTC CGACCCCATC CCTGAGGTAC GCTGCGCAGC CGTCTTTGCC 
CTCGGCACGT TTGTGGGTAA CTCTGCAGAG AGGACAGACC ACTCCACCAC CATCGACCAC 
AACGTGGCCA TGATGCTGGC CCAGCTCATC AATGACGGCA GCCCTGTGGT CCGGAAGGAG 
CTGGTGGTGG CTCTGAGTCA CCTTGTCGTT CAGTATGAAA GTAACTTCTG CACTGTGGCC 
CTGCAGTTCA TGGAAGAGGA GAAGAACTAC CCACTGCCCT CTCCAGCCAC CACAGAGGGT 
GGGAGTCTGA CCCCCGTGCG GGACAGCCCC TGCACCCCCA GACTGCGTTC CGTGAGCTCT 
TACGGGAATA TCCGCGCCGT CACCACGGCT AGAAACTTAA ACAAGTCTTT GCAAAACCTG 
AGCCTGACGG AAGAAACCGG TGGCTCGGTG GCCTTCTCCC CCGGGAACCT GAGCACCAGC 
AGCAGCGCCA GCAGCACCCT GGGCAGCCCC GAGAACGAGG AGTACATCCT GTCCTTCGAG 
ACCATTGACA AGATGCGCCG CGTCAGCTCC TACTCCTCCC TCAACTCCCT CATCGGAGTT 
TCATTCAATA GTGTTTACAC TCAAATTTGG AGAGTCTTGC TGCATCTGGC TGCGGACCCC 
TATCCAGATG TTTCTGACTT GGCTATGAAA GTGCTCAACA GCATTGCCTA CAAGGCCACA 
GTGAACGCCC GGCCTCAGCG CATCCTCAGC ACCTCCTCCC TCACGCAGTC GGCGCCTGCC 
AGCCCCACCA ACAAAGGCAT CCACATCCAC CAGGCGGGAG GCTCCCCTCC AACATCCAGC 
GCAAGCAGCT CCAGCCTGAC CAACGACGTG GCCAAGCAGC CGGTCAGCCG AGACCTGCCT 
CCAGGCCGGC CGGGCGCCAC AGGCCCCGTG GGGGTACAGT ACACACCCCA CTCGCACCAG 
TTCCCCCGGA CGCGGAAGAT GTTCGATAAA GGCCCAGACC AGACCACTGA TGACCCAGAT 
GATACTGCCG GACACAAAAG TTTCATCTCG GCCACGATGC AGACGGGGTT CTGCGACTGG 
AGCGCGCGGT ACTTTGCCCA GCCTGTCATG AAGATTCCGG AAGAGCATGA CCTGGAGAGT 
CAGATCCGCA AGGAGCGGGA GTGGCGGTTT CTGCGGAACA CCCGTGTCCG GAAGCAGGCA 
CAACAGCTCA TCCAGAAGGG TATCACAAGG CTGGACGACC AGATATTTCT GAACAGGAAC 
CCTGGCGTCC CCTCCGTGGT CAAGTTTCAC CCCTTTACAC CATGCGTGGC CGTCGCTGAC 
AAAGACAGCA TCTGCTTTTG GGACTGGGAG AAAGGGGAGA AGCTGGATTA TTTCCACAAC 
GGAAACCCTC GGTACACGAG GGTCACGGCC ATGGAGTATC TGAATGGCCA GGATTGCTCC 
CTCCTGCTCA CAGCCACAGA TGACGGTGCC ATCAGGGTCT GGAAGAACTT TGCTGATTTG 
GAAAAGAACC CAGAAATGGT GACAGCCTGG CAGGGACTCT CGGACATGCT GCCCACCACC 
CGAGGTCTGG CCCGAAAGGT CTCAATCTTT CTTGACCACA ATAAGCAGGG AGGAGCTGGG 
ATGGTGGTGG ACTGGGAGCA GGAGACCGGC CTCCTCATGA GCTCGGGAGA CGTGCGGATC 
ATCCGGATCT GGGACACAGA CCGAGAGATG AAGGTGCAGG ACATCCCCAC GGGCGCCGAC 
AGCTGCGTGA CGAGCCTCTC CTGCGACTCC CACCGCTCCC TCATTGTGGC CGGGCTCGGC 
GACGGCTCCA TCCGCGTCTA TGACAGGAGG ATGGCGCTCA GCGAATGCCG CGTCATGACC 
TACCGGGAGC ACACGGCCTG GGTGGTGAAG GCCTACCTCC AGAAGCACCC CGAGGGCCAC 
ATCATGAGCG TCAGCGTCAA CGGGGATGTG CGCTTCTTTG ACCCTCGGCT GCCGGAGTCT 
GTGAACGTCA TGCAGATCGT GAAGGGGCTG ACAGCGCTCG ACATCCACCC CCAGGCAAAC 
CTGATTGCGT GTGGCTCCAT GAACCAGTTC ACTGCCATCT ACAACGGCAG CGGAGAGCTG 
ATCAACCATA TCAAGTACTA CGACGGCTTC ATGGGCCAGC GGGTCGGTGC CATCAGCTGC 
CTGGCCTTCC ACCCACACTG GCCGCACCTG GCCGTGGGCA GCAATGACTA CTACATCTCC 
GTGTACTCGG TGGAGAAGCG TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314407.1
CDS	241..4050
Translation	MDVPLPDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEANELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLCSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPVVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPATTEGGSLTPVRDSPCTPRLRSVSSYGNIRAVTTARNLNKSLQNLSLTEETGGSVAFSPGNLSTSSSASSTLGSPENEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPDVSDLAMKVLNSIAYKATVNARPQRILSTSSLTQSAPASPTNKGIHIHQAGGSPPTSSASSSSLTNDVAKQPVSRDLPPGRPGATGPVGVQYTPHSHQFPRTRKMFDKGPDQTTDDPDDTAGHKSFISATMQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNTRVRKQAQQLIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCVAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARKVSIFLDHNKQGGAGMVVDWEQETGLLMSSGDVRIIRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSECRVMTYREHTAWVVKAYLQKHPEGHIMSVSVNGDVRFFDPRLPESVNVMQIVKGLTALDIHPQANLIACGSMNQFTAIYNGSGELINHIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Target ORF information:

RefSeq Version	XM_015458921.1
Organism	Bos taurus(cattle)
Definition	Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458921.1

ORF Insert Sequence:

ATGGATGTGC CACTACCGGA TCCTCTGTCG ATGGGTCCTC AGAAAGCTCT GGAAACCATT 
GGTGCAAATC TACAGAAGCA GTACGAGAAC TGGCAGCCAA GGGCGCGGTA TAAGCAGAGC 
CTTGATCCGA CTGTGGATGA AGTAAAGAAG CTTTGCACGT CTTTACGCCG AAATGCCAAG 
GAGGAGCGGG TCCTCTTCCA CTACAATGGC CATGGGGTGC CCAGGCCCAC CGTTAATGGC 
GAGATCTGGG TCTTCAACAA GAACTACACT CAGTACATCC CTCTGTCTAT ATACGACCTG 
CAGACGTGGA TGGGTAGCCC CTCCATATTC GTCTATGACT GCTCCAATGC TGGCCTCATT 
GTCAAGTCCT TCAAGCAGTT CGCCCTGCAG AGGGAGCAGG AGCTGGAGGT CGCTGCTATT 
AACCCAAACC ACCCACTGGC CCAGATGCCT TTGCCCCCGT CTATGAAGAA CTGCATTCAG 
CTGGCAGCCT GTGAGGCCAA CGAGCTCCTG CCCATGATCC CCGACCTCCC AGCTGACCTG 
TTCACATCAT GCCTCACCAC CCCCATCAAA ATCGCCCTGC GCTGGTTCTG CATGCAGAAA 
TGTGTTAGTC TGGTGCCTGG AGTCACACTG GATTTGATAG AAAAGATTCC CGGGCGGCTG 
AATGACAGGA GGACCCCCCT GGGCGAGCTG AACTGGATCT TCACAGCCAT CACGGACACG 
ATTGCGTGGA ACGTGCTTCC TCGGGATCTC TTCCAAAAGC TCTTCAGACA GGACCTGTTG 
GTGGCTAGTC TGTTTCGAAA TTTTTTATTG GCAGAAAGAA TCATGAGGTC ATATAACTGC 
ACCCCCGTCA GCAGTCCACG CCTACCCCCC ACGTACATGC ACGCCATGTG GCAAGCCTGG 
GACCTGGCCG TGGACATCTG TCTCTCCCAG CTGCCGACCA TCATCGAAGA AGGCACAGCA 
TTTCGGCACA GCCCCTTCTT TGCTGAGCAG CTGACCGCCT TCCAGGTGTG GCTCACCATG 
GGCGTGGAAA ACAGGAACCC ACCTGAGCAG CTTCCTATTG TCTTACAGGT GCTGCTGAGC 
CAAGTGCACC GGCTGAGAGC CCTGGACTTG CTGGGAAGAT TTCTGGACCT GGGCCCCTGG 
GCAGTGAGCC TGGCGCTGTC TGTGGGCATC TTCCCCTATG TGCTAAAGCT GCTCCAGAGC 
TCAGCCAGGG AGCTGCGGCC TCTCCTTGTC TTCATCTGGG CCAAGATCCT TGCCGTGGAC 
AGTTCATGCC AAGCTGACCT GGTGAAGGAC AACGGCCACA AGTACTTCCT CTCGGTCTTG 
GCAGACCCGT ACATGCCAGC TGAGCACCGG ACCATGACGG CCTTCATTCT GGCTGTAATC 
GTCAACAGCT ATAACACGGG GCAGGAAGCC TGCCTCCAGG GAAACCTGAT CGCCATCTGC 
CTGGAGCAGC TCAACGACCC CCACCCCCTG CTGCGCCAGT GGGTGGCCAT CTGCCTGGGC 
CGCATCTGGC AGAACTTTGA CTCGGCCCGG TGGTGCGGGG TGCGGGACAG CGCCCACGAG 
AAGCTCTGCA GCCTCCTCTC CGACCCCATC CCTGAGGTAC GCTGCGCAGC CGTCTTTGCC 
CTCGGCACGT TTGTGGGTAA CTCTGCAGAG AGGACAGACC ACTCCACCAC CATCGACCAC 
AACGTGGCCA TGATGCTGGC CCAGCTCATC AATGACGGCA GCCCTGTGGT CCGGAAGGAG 
CTGGTGGTGG CTCTGAGTCA CCTTGTCGTT CAGTATGAAA GTAACTTCTG CACTGTGGCC 
CTGCAGTTCA TGGAAGAGGA GAAGAACTAC CCACTGCCCT CTCCAGCCAC CACAGAGGGT 
GGGAGTCTGA CCCCCGTGCG GGACAGCCCC TGCACCCCCA GACTGCGTTC CGTGAGCTCT 
TACGGGAATA TCCGCGCCGT CACCACGGCT AGAAACTTAA ACAAGTCTTT GCAAAACCTG 
AGCCTGACGG AAGAAACCGG TGGCTCGGTG GCCTTCTCCC CCGGGAACCT GAGCACCAGC 
AGCAGCGCCA GCAGCACCCT GGGCAGCCCC GAGAACGAGG AGTACATCCT GTCCTTCGAG 
ACCATTGACA AGATGCGCCG CGTCAGCTCC TACTCCTCCC TCAACTCCCT CATCGGAGTT 
TCATTCAATA GTGTTTACAC TCAAATTTGG AGAGTCTTGC TGCATCTGGC TGCGGACCCC 
TATCCAGATG TTTCTGACTT GGCTATGAAA GTGCTCAACA GCATTGCCTA CAAGGCCACA 
GTGAACGCCC GGCCTCAGCG CATCCTCAGC ACCTCCTCCC TCACGCAGTC GGCGCCTGCC 
AGCCCCACCA ACAAAGGCAT CCACATCCAC CAGGCGGGAG GCTCCCCTCC AACATCCAGC 
GCAAGCAGCT CCAGCCTGAC CAACGACGTG GCCAAGCAGC CGGTCAGCCG AGACCTGCCT 
CCAGGCCGGC CGGGCGCCAC AGGCCCCGTG GGGGTACAGT ACACACCCCA CTCGCACCAG 
TTCCCCCGGA CGCGGAAGAT GTTCGATAAA GGCCCAGACC AGACCACTGA TGACCCAGAT 
GATACTGCCG GACACAAAAG TTTCATCTCG GCCACGATGC AGACGGGGTT CTGCGACTGG 
AGCGCGCGGT ACTTTGCCCA GCCTGTCATG AAGATTCCGG AAGAGCATGA CCTGGAGAGT 
CAGATCCGCA AGGAGCGGGA GTGGCGGTTT CTGCGGAACA CCCGTGTCCG GAAGCAGGCA 
CAACAGCTCA TCCAGAAGGG TATCACAAGG CTGGACGACC AGATATTTCT GAACAGGAAC 
CCTGGCGTCC CCTCCGTGGT CAAGTTTCAC CCCTTTACAC CATGCGTGGC CGTCGCTGAC 
AAAGACAGCA TCTGCTTTTG GGACTGGGAG AAAGGGGAGA AGCTGGATTA TTTCCACAAC 
GGAAACCCTC GGTACACGAG GGTCACGGCC ATGGAGTATC TGAATGGCCA GGATTGCTCC 
CTCCTGCTCA CAGCCACAGA TGACGGTGCC ATCAGGGTCT GGAAGAACTT TGCTGATTTG 
GAAAAGAACC CAGAAATGGT GACAGCCTGG CAGGGACTCT CGGACATGCT GCCCACCACC 
CGAGGTCTGG CCCGAAAGGT CTCAATCTTT CTTGACCACA ATAAGCAGGG AGGAGCTGGG 
ATGGTGGTGG ACTGGGAGCA GGAGACCGGC CTCCTCATGA GCTCGGGAGA CGTGCGGATC 
ATCCGGATCT GGGACACAGA CCGAGAGATG AAGGTGCAGG ACATCCCCAC GGGCGCCGAC 
AGCTGCGTGA CGAGCCTCTC CTGCGACTCC CACCGCTCCC TCATTGTGGC CGGGCTCGGC 
GACGGCTCCA TCCGCGTCTA TGACAGGAGG ATGGCGCTCA GCGAATGCCG CGTCATGACC 
TACCGGGAGC ACACGGCCTG GGTGGTGAAG GCCTACCTCC AGAAGCACCC CGAGGGCCAC 
ATCATGAGCG TCAGCGTCAA CGGGGATGTG CGCTTCTTTG ACCCTCGGCT GCCGGAGTCT 
GTGAACGTCA TGCAGATCGT GAAGGGGCTG ACAGCGCTCG ACATCCACCC CCAGGCAAAC 
CTGATTGCGT GTGGCTCCAT GAACCAGTTC ACTGCCATCT ACAACGGCAG CGGAGAGCTG 
ATCAACCATA TCAAGTACTA CGACGGCTTC ATGGGCCAGC GGGTCGGTGC CATCAGCTGC 
CTGGCCTTCC ACCCACACTG GCCGCACCTG GCCGTGGGCA GCAATGACTA CTACATCTCC 
GTGTACTCGG TGGAGAAGCG TGTCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPTOR ( NM_001192130.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179059.1 Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), mRNA.

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CloneID	OBa40069
Clone ID Related Accession (Same CDS sequence)	NM_001192130.1
Accession Version	NM_001192130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	regulatory-associated protein of mTOR
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC02062143.1, AAFC03012694.1, AAFC03012693.1, AAFC03012692.1, AAFC03035398.1 and AAFC03035399.1. On Jul 17, 2010 this sequence version replaced XM_583606.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTGCC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACTGATGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC 
TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC 
ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC 
ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC 
GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG 
AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC 
CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC 
ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC 
AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG 
GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC 
AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179059.1
CDS	758..4765
Translation	MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEANELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLCSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPVVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPATTEGGSLTPVRDSPCTPRLRSVSSYGNIRAVTTARNLNKSLQNLSLTEETGGSVAFSPGNLSTSSSASSTLGSPENEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPDVSDLAMKVLNSIAYKATVNARPQRILSTSSLTQSAPASPTNKGIHIHQAGGSPPTSSASSSSLTNDVAKQPVSRDLPPGRPGTTGPVGVQYTPHSHQFPRTRKMFDKGPDQTTDDPDDTAGHKSFISATMQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNTRVRKQAQQLIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCVAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMSSGDVRIIRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSECRVMTYREHTAWVVKAYLQKHPEGHIMSVSVNGDVRFFDPRLPESVNVMQIVKGLTALDIHPQANLIACGSMNQFTAIYNGSGELINHIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Target ORF information:

RefSeq Version	NM_001192130.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192130.1

ORF Insert Sequence:

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTGCC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACTGATGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC 
TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC 
ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC 
ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC 
GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG 
AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC 
CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC 
ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC 
AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG 
GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC 
AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPTOR ( XM_005221019.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221076.1 Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30234
Clone ID Related Accession (Same CDS sequence)	XM_005221019.3
Accession Version	XM_005221019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulatory-associated protein of mTOR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104498.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GTCTGGCCCG AAAGGTCTCA ATCTTTCTTG ACCACAATAA GCAGGGAGGA 
GCTGGGATGG TGGTGGACTG GGAGCAGGAG ACCGGCCTCC TCATGAGCTC GGGAGACGTG 
CGGATCATCC GGATCTGGGA CACAGACCGA GAGATGAAGG TGCAGGACAT CCCCACGGGC 
GCCGACAGCT GCGTGACGAG CCTCTCCTGC GACTCCCACC GCTCCCTCAT TGTGGCCGGG 
CTCGGCGACG GCTCCATCCG CGTCTATGAC AGGAGGATGG CGCTCAGCGA ATGCCGCGTC 
ATGACCTACC GGGAGCACAC GGCCTGGGTG GTGAAGGCCT ACCTCCAGAA GCACCCCGAG 
GGCCACATCA TGAGCGTCAG CGTCAACGGG GATGTGCGCT TCTTTGACCC TCGGCTGCCG 
GAGTCTGTGA ACGTCATGCA GATCGTGAAG GGGCTGACAG CGCTCGACAT CCACCCCCAG 
GCAAACCTGA TTGCGTGTGG CTCCATGAAC CAGTTCACTG CCATCTACAA CGGCAGCGGA 
GAGCTGATCA ACCATATCAA GTACTACGAC GGCTTCATGG GCCAGCGGGT CGGTGCCATC 
AGCTGCCTGG CCTTCCACCC ACACTGGCCG CACCTGGCCG TGGGCAGCAA TGACTACTAC 
ATCTCCGTGT ACTCGGTGGA GAAGCGTGTC AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221076.1
CDS	1213..5268
Translation	MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEANELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLCSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPVVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPATTEGGSLTPVRDSPCTPRLRSVSSYGNIRAVTTARNLNKSLQNLSLTEETGGSVAFSPGNLSTSSSASSTLGSPENEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPDVSDLAMKVLNSIAYKATVNARPQRILSTSSLTQSAPASPTNKGIHIHQAGGSPPTSSASSSSLTNDVAKQPVSRDLPPGRPGATGPVGVQYTPHSHQFPRTRKMFDKGPDQTTDDPDDTAGHKSFISATMQTGFCDWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNTRVRKQAQQLIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCVAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARKVSIFLDHNKQGGAGMVVDWEQETGLLMSSGDVRIIRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALSECRVMTYREHTAWVVKAYLQKHPEGHIMSVSVNGDVRFFDPRLPESVNVMQIVKGLTALDIHPQANLIACGSMNQFTAIYNGSGELINHIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Target ORF information:

RefSeq Version	XM_005221019.3
Organism	Bos taurus(cattle)
Definition	Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221019.3

ORF Insert Sequence:

ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA 
GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT 
GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT 
TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA 
ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG 
CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT 
GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT 
AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC 
GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC 
CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT 
GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC 
ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT 
GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG 
CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG 
CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG 
GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC 
CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT 
AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA 
GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC 
ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC 
ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG 
CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC 
CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC 
CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC 
GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG 
GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT 
GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC 
ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC 
CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC 
CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC 
TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC 
GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG 
AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT 
GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA 
GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG 
AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA 
AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC 
ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC 
TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC 
GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG 
GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG 
GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG 
CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA 
TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC 
CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG 
CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC 
CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC 
GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG 
GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG 
CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC 
AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC 
GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC 
CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT 
TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT 
GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC 
ACCACCCGAG GTCTGGCCCG AAAGGTCTCA ATCTTTCTTG ACCACAATAA GCAGGGAGGA 
GCTGGGATGG TGGTGGACTG GGAGCAGGAG ACCGGCCTCC TCATGAGCTC GGGAGACGTG 
CGGATCATCC GGATCTGGGA CACAGACCGA GAGATGAAGG TGCAGGACAT CCCCACGGGC 
GCCGACAGCT GCGTGACGAG CCTCTCCTGC GACTCCCACC GCTCCCTCAT TGTGGCCGGG 
CTCGGCGACG GCTCCATCCG CGTCTATGAC AGGAGGATGG CGCTCAGCGA ATGCCGCGTC 
ATGACCTACC GGGAGCACAC GGCCTGGGTG GTGAAGGCCT ACCTCCAGAA GCACCCCGAG 
GGCCACATCA TGAGCGTCAG CGTCAACGGG GATGTGCGCT TCTTTGACCC TCGGCTGCCG 
GAGTCTGTGA ACGTCATGCA GATCGTGAAG GGGCTGACAG CGCTCGACAT CCACCCCCAG 
GCAAACCTGA TTGCGTGTGG CTCCATGAAC CAGTTCACTG CCATCTACAA CGGCAGCGGA 
GAGCTGATCA ACCATATCAA GTACTACGAC GGCTTCATGG GCCAGCGGGT CGGTGCCATC 
AGCTGCCTGG CCTTCCACCC ACACTGGCCG CACCTGGCCG TGGGCAGCAA TGACTACTAC 
ATCTCCGTGT ACTCGGTGGA GAAGCGTGTC AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Energy depletion of bovine mammary epithelial cells activates AMPK and suppresses protein synthesis through inhibition of mTORC1 signaling.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(3)183-9(2013 Mar)
Burgos SA,Kim JJ,Dai M,Cant JP

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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RPTOR cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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