RALGAPA2 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following RALGAPA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RALGAPA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221909 XM_025001040.1
Latest version!
Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221910 XM_025001041.1
Latest version!
Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221911 XM_025001042.1
Latest version!
Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221912 XM_025001043.1
Latest version!
Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221913 XM_025001044.1
Latest version!
Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221914 XM_025001045.1
Latest version!
Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa92268 XM_015474043.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92269 XM_015474044.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474052.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474050.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474051.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474049.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474053.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474045.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474047.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474048.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92270 XM_015474046.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92271 XM_015474056.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92271 XM_015474055.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92271 XM_015474054.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92271 XM_015474057.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92272 XM_015474060.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92272 XM_015474058.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92272 XM_015474059.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92272 XM_015474061.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92273 XM_015474062.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92274 XM_015474063.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa92275 XM_015474064.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94787 XM_015465968.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94788 XM_015465969.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_015465972.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_010822472.2
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_015465973.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_015465971.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_015465970.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_010822473.2
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_015465976.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_015465975.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94789 XM_015465974.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94790 XM_015465979.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94790 XM_015465978.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94790 XM_015465980.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94790 XM_015465977.1
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94791 XM_010822477.2
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94791 XM_010822474.2
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94791 XM_010822476.2
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94791 XM_010822475.2
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94792 XM_010822479.2
Latest version!
Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221909
    Clone ID Related Accession (Same CDS sequence) XM_025001040.1
    Accession Version XM_025001040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
    AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC
    AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA
    AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG
    GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT
    TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA
    AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT
    CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT
    GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC
    ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG
    GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT
    ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT
    CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA
    ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC
    ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT
    GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA
    CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC
    ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA
    GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC
    ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC
    GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC
    ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT
    GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA
    TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC
    AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG
    GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT
    GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT
    GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT
    AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG
    AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT
    AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC
    TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA
    TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG
    ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA
    GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG
    CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC
    GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGCTAAGCGA
    AGCCAGTCTA TCAGCAACTG TGCCCACCTC TCTGAGGCTT TGCCCGCTAC TAAAAGCGTC
    CCACTCCTCC TCCACACCGT GAGCGCTCTC TTCCCTGGTC TCTCTTACTC TTCTTGCTCC
    CACAGGCTGT CAGAAGTGGA AGAATACCTG CAGCCAGAAA ACACACCTGC CACCGAATCT
    GGGCCCCTGG GGCAGCAGCC GATGGTTCGG AGCGGCAGCA GCCCTGAGGT CACCGAGCCG
    CTGTGTCCTG AGCCCCCCCC AGGCCAAAAG CTAGAAAATG CACAGAACGT GAGTTCTTCA
    GAGCCCAGAC TTGCTCAGGA GAGCAAAGGA CATGAGAAGA AAGAGCATGA AGGAATAACA
    ATCTTAGTTC GAAGAAGCAG CAGCCCCATT GAACTGGATT TGAAAGATGA CTCACAGCAG
    ATGCAAGGAA AACGTAGGGA GAGACAGAAG AGTGACAGTG TGAGCAGTGA CCCCACTCTG
    GGCTGCAGCA CTGAGGCAGA GCTGTCCCTG AGCCACTGGC AGACCTGCGA GGAAGACCCA
    GATCTGAGCC CGCCTGCAGA TACTGTGACC GACGCTGACG CCCGCCACTG GTTACAGCTC
    CGGCCCACGG ATGCTTCAAA CCTAACAGAC AGCAGCGAAT GCCTTGCTGA TGACTGCAGT
    ATAATTGCTG GGGGGACCCT CACTGGTTGG CACCCAGACT CAGCCGCTGT GTTGTGGCGA
    AGAATCTTGG GAATCCTTGG AGATGTGAAT AATATCCAGT CACCCAAAAT CCATGCCAGG
    GTGTTTGGCT ATCTCTATGA ACTCTGGTAC AAGCTAGCAA AGATACGGGA TAACCTGGCA
    ATAAGCCTGG ATAACCAGTC TTCTCCATCT CCTCCTGCCT TGATCCCACC GCTCAGAATG
    TTTGCATCAT GGCTATTTAA GGCGACGACG ATGCCTGACG ATTATAAGGA AGGCAAACTG
    CAAGCCTATA AGCTGATCTG CTCCATGATG ACCCGGCGCC AGGACGTCCT GCCCAACTCA
    GACTTCCTGG TGCATTTTTA CCTCGTCATG CACCTGGGAC TGACCAGCGA GGACCAGGAT
    ATCCTGAATA CCATCATAAG GCACTGCCCG CCCCGCTTCT TCTCCCTGGG CTTGCCTGGC
    TTCTCCATGC TGGTGGGGGA CTTCATCATG GCTGCTGGCA GGGTCCTCAG TGCAGACACG
    CTGGCGGCAC CTCGTTCGGA AGCCCTCACC ATTCTCGGGT CTCTGGTCTG CTTTCCGAAC
    ACCTACCGCG AGATTCCCCT ACGGCAGCCG GTACCTGAAG TTGCTGAAGT CGCCATGGGA
    GCAGAAGATG TCAAGCACTG CCTCATAAGT ATCTTACTGA AGAATGCCAC AGAGGAACCG
    AGTGAGTGTG CAAGGTGCAT TGCCATCTGC TCCCTCGGGA TCTGGATCTG TGAGGAGCTG
    GCACAAGGCA CTGGCCACCC TCAGCTGCAA GAGGCCGTCA ACGTCATTGG TGTCACGTTG
    AAGTTTCCTA ACAAAATTGT GGCCCAGGTA GCCTGTGACG TGCTTCAGCT GCTAGTCTCC
    TACTGGGAAA AGCTTCAGAT GTTTGAAACC TCCTTGCCTC GGAAAATTGC AGAAATCCTT
    GTGGCCACAA TTGCTTTTCT GTTACCAAAT GCAGAGTACT CCTCGGTGGA GACAGACAAG
    AAGTTCCTCG TGTCCTTGTT GCTCTGCCTC CTGGACTGGT GCATGGCGCT GCCGGCAGGA
    ACCCTCCTCC ACCCCGTGTC CACGGCGGCC CTGGACGAAC AGGCCCCGGC CCGGGCCCCC
    TTACTGGATT ACATCTACAG GGTTCTGCAC TGCTGCATCT GTGGCTCAAG CACGTACAGC
    CAGCAGAGCC GCTACACCTT GACCCTGGCG GACCTGTCGT CCTCGGATTA CGACCCCTTC
    CTGCCGCTGG CGAACGTGAA GAATTCTGAG CCGGTCCAAT TTCATTCATC AGCAGAACTG
    GGCAACCTGC TCACTGTTGA AGAGGCAGAG AGGAGGAGAA GGAGCCTGGG GCTGATCCCG
    CTGACAGCGC GCATGGTGAT GGCGCACCTG GTCAACCACC TGGGCCACTT CCCGCTCTGC
    GGGGGCCCCG CCGTGCTGCA CAGCCTGGTG GGCGAGCACC ACGACAACGT GCACGCCGAC
    GCCGCCGAGC TCTCCCCAGA CGTGTTCAGG AGCCCCAGCC TGCAGCTGTT TGTGTTCAAC
    GACAGCACCC TCATCTCCTA CCTGCAGACG CCTGCGACCG GTCCTGCCAG TGGGTCGCCA
    GCGGGCGCCC CCTCGGACGT GAGAGTGATC GTGAGGGACA TCTCAGGGAA GTACTCGTGG
    GACGGGAAGG TGCTCTACGG ACCCCTGGAA GGCCACTTAG CCCCCAAGGG CAGAAGGCCC
    TCGTTCCTCA TCTCGAGCTG TCAACCCCCA ACCCCTGGAC CTCAGAGGGC TCCTTCTCAG
    ACTGAGGAGG GAGAGGATGT CCTCGACAAA TTACTTGAAA ACCTTGGCCA CACGAGTCCT
    GAATGCCTTT TGCCATCCCA GCTCCGTCTG AATGAGCCTT CCCCACCCCC TTGTGGCATG
    AATTGGGACC AGGAGAAGGA GATCACCAAG GTCCTGCTGC GCCAGAGCAC GCAAGAAGAC
    GAGTATGTCC AGAGGTGCAA CTCGGATGTG GCCATGAGGG TCGCCAGCCA GGAGCCGCCG
    TCCCCAGTGG AGCCCCGGGG TGCCTTCTAC TTCTGTAGGC TGCTGCTCGA TGACTTGGGA
    ATGAATTCTT GGGACAGAAG GAAGAACTTC CATCTGTTGA AGAAAAATTC AAAATTATTG
    AGAGAGCTAA AAAATCTGGA CTCCCGGCAG TGCCGTGAAA CGCACAAAAT CGCAGTGTTT
    TACATTGCCG AAGGTCAAGA AGACAAGTGT TCGATCCTTT CCAATGAAAG GGGAAGCCAA
    GCGTATGAAG ACTTTGTTGC TGGACTTGGA TGGGAGGTGG ACCTGTCAAC CCACTGTGGG
    TTCATGGGCG GGCTTCAGCG CAATGGCAGC ACGGGCCAGA CCGCCCCCTA CTACGCTACC
    TCGACCGTGG AAGTCATCTT CCACGTCTCA ACTCGGATGC CGTCAGACTC AGACGACTCG
    CTCACCAAAA AGCTCCGTCA CTTGGGGAAT GACGAAGTCC ACATCGTCTG GTCCGAGCAC
    TCCAGGGACT ACCGCAGCGG GATCATCCCC ACCGCCTTCG GAGACGTTTC AATCATCATC
    TACCCCATGA AGAATCACAT GTTCTTTATC GCGATAACCA AGAAACCCGA GGTTCCATTT
    TTTGGGCCTC TGTTTGATGG AGCAATCGTG AGTGGGAAGC TACTGCCAAG CCTTATCTGT
    GCCACCTGCA TCAACGCCAG CCGGGCCGTC AAGTGCCTCA TCCCCCTCTA CCAGAGCTTC
    TACGAGGAGA GAGCTCTCTA TCTGGAAGCA ATAATTCAGA ACCACCGTGA AGTCATGACA
    TTTGAGGATT TCACAGCCCA GGTCTTCTGT CCATCTCCCA GCTACTCGGT CAGCGGAACT
    GTGTCAGCTC TGGAAGAGGG AAGGGATGGA TGTGATGAAC ATCTGCTCCA CCTGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856808.1
    CDS117..5936
    Translation

    Target ORF information:

    RefSeq Version XM_025001040.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
    AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC
    AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA
    AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG
    GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT
    TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA
    AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT
    CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT
    GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC
    ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG
    GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT
    ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT
    CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA
    ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC
    ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT
    GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA
    CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC
    ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA
    GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC
    ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC
    GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC
    ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT
    GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA
    TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC
    AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG
    GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT
    GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT
    GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT
    AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG
    AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT
    AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC
    TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA
    TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG
    ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA
    GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG
    CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC
    GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGCTAAGCGA
    AGCCAGTCTA TCAGCAACTG TGCCCACCTC TCTGAGGCTT TGCCCGCTAC TAAAAGCGTC
    CCACTCCTCC TCCACACCGT GAGCGCTCTC TTCCCTGGTC TCTCTTACTC TTCTTGCTCC
    CACAGGCTGT CAGAAGTGGA AGAATACCTG CAGCCAGAAA ACACACCTGC CACCGAATCT
    GGGCCCCTGG GGCAGCAGCC GATGGTTCGG AGCGGCAGCA GCCCTGAGGT CACCGAGCCG
    CTGTGTCCTG AGCCCCCCCC AGGCCAAAAG CTAGAAAATG CACAGAACGT GAGTTCTTCA
    GAGCCCAGAC TTGCTCAGGA GAGCAAAGGA CATGAGAAGA AAGAGCATGA AGGAATAACA
    ATCTTAGTTC GAAGAAGCAG CAGCCCCATT GAACTGGATT TGAAAGATGA CTCACAGCAG
    ATGCAAGGAA AACGTAGGGA GAGACAGAAG AGTGACAGTG TGAGCAGTGA CCCCACTCTG
    GGCTGCAGCA CTGAGGCAGA GCTGTCCCTG AGCCACTGGC AGACCTGCGA GGAAGACCCA
    GATCTGAGCC CGCCTGCAGA TACTGTGACC GACGCTGACG CCCGCCACTG GTTACAGCTC
    CGGCCCACGG ATGCTTCAAA CCTAACAGAC AGCAGCGAAT GCCTTGCTGA TGACTGCAGT
    ATAATTGCTG GGGGGACCCT CACTGGTTGG CACCCAGACT CAGCCGCTGT GTTGTGGCGA
    AGAATCTTGG GAATCCTTGG AGATGTGAAT AATATCCAGT CACCCAAAAT CCATGCCAGG
    GTGTTTGGCT ATCTCTATGA ACTCTGGTAC AAGCTAGCAA AGATACGGGA TAACCTGGCA
    ATAAGCCTGG ATAACCAGTC TTCTCCATCT CCTCCTGCCT TGATCCCACC GCTCAGAATG
    TTTGCATCAT GGCTATTTAA GGCGACGACG ATGCCTGACG ATTATAAGGA AGGCAAACTG
    CAAGCCTATA AGCTGATCTG CTCCATGATG ACCCGGCGCC AGGACGTCCT GCCCAACTCA
    GACTTCCTGG TGCATTTTTA CCTCGTCATG CACCTGGGAC TGACCAGCGA GGACCAGGAT
    ATCCTGAATA CCATCATAAG GCACTGCCCG CCCCGCTTCT TCTCCCTGGG CTTGCCTGGC
    TTCTCCATGC TGGTGGGGGA CTTCATCATG GCTGCTGGCA GGGTCCTCAG TGCAGACACG
    CTGGCGGCAC CTCGTTCGGA AGCCCTCACC ATTCTCGGGT CTCTGGTCTG CTTTCCGAAC
    ACCTACCGCG AGATTCCCCT ACGGCAGCCG GTACCTGAAG TTGCTGAAGT CGCCATGGGA
    GCAGAAGATG TCAAGCACTG CCTCATAAGT ATCTTACTGA AGAATGCCAC AGAGGAACCG
    AGTGAGTGTG CAAGGTGCAT TGCCATCTGC TCCCTCGGGA TCTGGATCTG TGAGGAGCTG
    GCACAAGGCA CTGGCCACCC TCAGCTGCAA GAGGCCGTCA ACGTCATTGG TGTCACGTTG
    AAGTTTCCTA ACAAAATTGT GGCCCAGGTA GCCTGTGACG TGCTTCAGCT GCTAGTCTCC
    TACTGGGAAA AGCTTCAGAT GTTTGAAACC TCCTTGCCTC GGAAAATTGC AGAAATCCTT
    GTGGCCACAA TTGCTTTTCT GTTACCAAAT GCAGAGTACT CCTCGGTGGA GACAGACAAG
    AAGTTCCTCG TGTCCTTGTT GCTCTGCCTC CTGGACTGGT GCATGGCGCT GCCGGCAGGA
    ACCCTCCTCC ACCCCGTGTC CACGGCGGCC CTGGACGAAC AGGCCCCGGC CCGGGCCCCC
    TTACTGGATT ACATCTACAG GGTTCTGCAC TGCTGCATCT GTGGCTCAAG CACGTACAGC
    CAGCAGAGCC GCTACACCTT GACCCTGGCG GACCTGTCGT CCTCGGATTA CGACCCCTTC
    CTGCCGCTGG CGAACGTGAA GAATTCTGAG CCGGTCCAAT TTCATTCATC AGCAGAACTG
    GGCAACCTGC TCACTGTTGA AGAGGCAGAG AGGAGGAGAA GGAGCCTGGG GCTGATCCCG
    CTGACAGCGC GCATGGTGAT GGCGCACCTG GTCAACCACC TGGGCCACTT CCCGCTCTGC
    GGGGGCCCCG CCGTGCTGCA CAGCCTGGTG GGCGAGCACC ACGACAACGT GCACGCCGAC
    GCCGCCGAGC TCTCCCCAGA CGTGTTCAGG AGCCCCAGCC TGCAGCTGTT TGTGTTCAAC
    GACAGCACCC TCATCTCCTA CCTGCAGACG CCTGCGACCG GTCCTGCCAG TGGGTCGCCA
    GCGGGCGCCC CCTCGGACGT GAGAGTGATC GTGAGGGACA TCTCAGGGAA GTACTCGTGG
    GACGGGAAGG TGCTCTACGG ACCCCTGGAA GGCCACTTAG CCCCCAAGGG CAGAAGGCCC
    TCGTTCCTCA TCTCGAGCTG TCAACCCCCA ACCCCTGGAC CTCAGAGGGC TCCTTCTCAG
    ACTGAGGAGG GAGAGGATGT CCTCGACAAA TTACTTGAAA ACCTTGGCCA CACGAGTCCT
    GAATGCCTTT TGCCATCCCA GCTCCGTCTG AATGAGCCTT CCCCACCCCC TTGTGGCATG
    AATTGGGACC AGGAGAAGGA GATCACCAAG GTCCTGCTGC GCCAGAGCAC GCAAGAAGAC
    GAGTATGTCC AGAGGTGCAA CTCGGATGTG GCCATGAGGG TCGCCAGCCA GGAGCCGCCG
    TCCCCAGTGG AGCCCCGGGG TGCCTTCTAC TTCTGTAGGC TGCTGCTCGA TGACTTGGGA
    ATGAATTCTT GGGACAGAAG GAAGAACTTC CATCTGTTGA AGAAAAATTC AAAATTATTG
    AGAGAGCTAA AAAATCTGGA CTCCCGGCAG TGCCGTGAAA CGCACAAAAT CGCAGTGTTT
    TACATTGCCG AAGGTCAAGA AGACAAGTGT TCGATCCTTT CCAATGAAAG GGGAAGCCAA
    GCGTATGAAG ACTTTGTTGC TGGACTTGGA TGGGAGGTGG ACCTGTCAAC CCACTGTGGG
    TTCATGGGCG GGCTTCAGCG CAATGGCAGC ACGGGCCAGA CCGCCCCCTA CTACGCTACC
    TCGACCGTGG AAGTCATCTT CCACGTCTCA ACTCGGATGC CGTCAGACTC AGACGACTCG
    CTCACCAAAA AGCTCCGTCA CTTGGGGAAT GACGAAGTCC ACATCGTCTG GTCCGAGCAC
    TCCAGGGACT ACCGCAGCGG GATCATCCCC ACCGCCTTCG GAGACGTTTC AATCATCATC
    TACCCCATGA AGAATCACAT GTTCTTTATC GCGATAACCA AGAAACCCGA GGTTCCATTT
    TTTGGGCCTC TGTTTGATGG AGCAATCGTG AGTGGGAAGC TACTGCCAAG CCTTATCTGT
    GCCACCTGCA TCAACGCCAG CCGGGCCGTC AAGTGCCTCA TCCCCCTCTA CCAGAGCTTC
    TACGAGGAGA GAGCTCTCTA TCTGGAAGCA ATAATTCAGA ACCACCGTGA AGTCATGACA
    TTTGAGGATT TCACAGCCCA GGTCTTCTGT CCATCTCCCA GCTACTCGGT CAGCGGAACT
    GTGTCAGCTC TGGAAGAGGG AAGGGATGGA TGTGATGAAC ATCTGCTCCA CCTGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221910
    Clone ID Related Accession (Same CDS sequence) XM_025001041.1
    Accession Version XM_025001041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
    GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA
    TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA
    TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT
    AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG
    TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG
    AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT
    CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC
    CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC
    ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG
    CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG
    CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG
    GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT
    CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA
    CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC
    CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA
    AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT
    CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG
    GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC
    GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC
    ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT
    TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG
    ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA
    GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC
    AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA
    GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG
    GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG
    CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA
    ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA
    ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC
    TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG
    TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG
    GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG
    ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA
    AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG
    TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC
    AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG
    AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG
    CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG
    TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG
    CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC
    TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG
    CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC
    TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT
    CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG
    CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA
    ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA
    ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG
    TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA
    AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT
    GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA
    GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC
    TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC
    CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC
    TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG
    GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC
    TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA
    GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT
    GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA
    CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG
    TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC
    TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG
    GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG
    TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC
    CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA
    CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG
    CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG
    CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC
    AACCTGCTCA CTGTTGAAGA GGCAGAGAGG AGGAGAAGGA GCCTGGGGCT GATCCCGCTG
    ACAGCGCGCA TGGTGATGGC GCACCTGGTC AACCACCTGG GCCACTTCCC GCTCTGCGGG
    GGCCCCGCCG TGCTGCACAG CCTGGTGGGC GAGCACCACG ACAACGTGCA CGCCGACGCC
    GCCGAGCTCT CCCCAGACGT GTTCAGGAGC CCCAGCCTGC AGCTGTTTGT GTTCAACGAC
    AGCACCCTCA TCTCCTACCT GCAGACGCCT GCGACCGGTC CTGCCAGTGG GTCGCCAGCG
    GGCGCCCCCT CGGACGTGAG AGTGATCGTG AGGGACATCT CAGGGAAGTA CTCGTGGGAC
    GGGAAGGTGC TCTACGGACC CCTGGAAGGC CACTTAGCCC CCAAGGGCAG AAGGCCCTCG
    TTCCTCATCT CGAGCTGTCA ACCCCCAACC CCTGGACCTC AGAGGGCTCC TTCTCAGACT
    GAGGAGGGAG AGGATGTCCT CGACAAATTA CTTGAAAACC TTGGCCACAC GAGTCCTGAA
    TGCCTTTTGC CATCCCAGCT CCGTCTGAAT GAGCCTTCCC CACCCCCTTG TGGCATGAAT
    TGGGACCAGG AGAAGGAGAT CACCAAGGTC CTGCTGCGCC AGAGCACGCA AGAAGACGAG
    TATGTCCAGA GGTGCAACTC GGATGTGGCC ATGAGGGTCG CCAGCCAGGA GCCGCCGTCC
    CCAGTGGAGC CCCGGGGTGC CTTCTACTTC TGTAGGCTGC TGCTCGATGA CTTGGGAATG
    AATTCTTGGG ACAGAAGGAA GAACTTCCAT CTGTTGAAGA AAAATTCAAA ATTATTGAGA
    GAGCTAAAAA ATCTGGACTC CCGGCAGTGC CGTGAAACGC ACAAAATCGC AGTGTTTTAC
    ATTGCCGAAG GTCAAGAAGA CAAGTGTTCG ATCCTTTCCA ATGAAAGGGG AAGCCAAGCG
    TATGAAGACT TTGTTGCTGG ACTTGGATGG GAGGTGGACC TGTCAACCCA CTGTGGGTTC
    ATGGGCGGGC TTCAGCGCAA TGGCAGCACG GGCCAGACCG CCCCCTACTA CGCTACCTCG
    ACCGTGGAAG TCATCTTCCA CGTCTCAACT CGGATGCCGT CAGACTCAGA CGACTCGCTC
    ACCAAAAAGC TCCGTCACTT GGGGAATGAC GAAGTCCACA TCGTCTGGTC CGAGCACTCC
    AGGGACTACC GCAGCGGGAT CATCCCCACC GCCTTCGGAG ACGTTTCAAT CATCATCTAC
    CCCATGAAGA ATCACATGTT CTTTATCGCG ATAACCAAGA AACCCGAGGT TCCATTTTTT
    GGGCCTCTGT TTGATGGAGC AATCGTGAGT GGGAAGCTAC TGCCAAGCCT TATCTGTGCC
    ACCTGCATCA ACGCCAGCCG GGCCGTCAAG TGCCTCATCC CCCTCTACCA GAGCTTCTAC
    GAGGAGAGAG CTCTCTATCT GGAAGCAATA ATTCAGAACC ACCGTGAAGT CATGACATTT
    GAGGATTTCA CAGCCCAGGT CTTCTGTCCA TCTCCCAGCT ACTCGGTCAG CGGAACTGTG
    TCAGCTCTGG AAGAGGGAAG GGATGGATGT GATGAACATC TGCTCCACCT GATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856809.1
    CDS43..5739
    Translation

    Target ORF information:

    RefSeq Version XM_025001041.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
    GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA
    TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA
    TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT
    AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG
    TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG
    AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT
    CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC
    CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC
    ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG
    CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG
    CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG
    GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT
    CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA
    CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC
    CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA
    AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT
    CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG
    GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC
    GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC
    ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT
    TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG
    ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA
    GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC
    AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA
    GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG
    GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG
    CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA
    ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA
    ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC
    TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG
    TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG
    GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG
    ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA
    AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG
    TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC
    AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG
    AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG
    CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG
    TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG
    CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC
    TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG
    CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC
    TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT
    CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG
    CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA
    ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA
    ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG
    TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA
    AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT
    GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA
    GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC
    TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC
    CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC
    TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG
    GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC
    TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA
    GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT
    GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA
    CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG
    TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC
    TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG
    GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG
    TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC
    CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA
    CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG
    CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG
    CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC
    AACCTGCTCA CTGTTGAAGA GGCAGAGAGG AGGAGAAGGA GCCTGGGGCT GATCCCGCTG
    ACAGCGCGCA TGGTGATGGC GCACCTGGTC AACCACCTGG GCCACTTCCC GCTCTGCGGG
    GGCCCCGCCG TGCTGCACAG CCTGGTGGGC GAGCACCACG ACAACGTGCA CGCCGACGCC
    GCCGAGCTCT CCCCAGACGT GTTCAGGAGC CCCAGCCTGC AGCTGTTTGT GTTCAACGAC
    AGCACCCTCA TCTCCTACCT GCAGACGCCT GCGACCGGTC CTGCCAGTGG GTCGCCAGCG
    GGCGCCCCCT CGGACGTGAG AGTGATCGTG AGGGACATCT CAGGGAAGTA CTCGTGGGAC
    GGGAAGGTGC TCTACGGACC CCTGGAAGGC CACTTAGCCC CCAAGGGCAG AAGGCCCTCG
    TTCCTCATCT CGAGCTGTCA ACCCCCAACC CCTGGACCTC AGAGGGCTCC TTCTCAGACT
    GAGGAGGGAG AGGATGTCCT CGACAAATTA CTTGAAAACC TTGGCCACAC GAGTCCTGAA
    TGCCTTTTGC CATCCCAGCT CCGTCTGAAT GAGCCTTCCC CACCCCCTTG TGGCATGAAT
    TGGGACCAGG AGAAGGAGAT CACCAAGGTC CTGCTGCGCC AGAGCACGCA AGAAGACGAG
    TATGTCCAGA GGTGCAACTC GGATGTGGCC ATGAGGGTCG CCAGCCAGGA GCCGCCGTCC
    CCAGTGGAGC CCCGGGGTGC CTTCTACTTC TGTAGGCTGC TGCTCGATGA CTTGGGAATG
    AATTCTTGGG ACAGAAGGAA GAACTTCCAT CTGTTGAAGA AAAATTCAAA ATTATTGAGA
    GAGCTAAAAA ATCTGGACTC CCGGCAGTGC CGTGAAACGC ACAAAATCGC AGTGTTTTAC
    ATTGCCGAAG GTCAAGAAGA CAAGTGTTCG ATCCTTTCCA ATGAAAGGGG AAGCCAAGCG
    TATGAAGACT TTGTTGCTGG ACTTGGATGG GAGGTGGACC TGTCAACCCA CTGTGGGTTC
    ATGGGCGGGC TTCAGCGCAA TGGCAGCACG GGCCAGACCG CCCCCTACTA CGCTACCTCG
    ACCGTGGAAG TCATCTTCCA CGTCTCAACT CGGATGCCGT CAGACTCAGA CGACTCGCTC
    ACCAAAAAGC TCCGTCACTT GGGGAATGAC GAAGTCCACA TCGTCTGGTC CGAGCACTCC
    AGGGACTACC GCAGCGGGAT CATCCCCACC GCCTTCGGAG ACGTTTCAAT CATCATCTAC
    CCCATGAAGA ATCACATGTT CTTTATCGCG ATAACCAAGA AACCCGAGGT TCCATTTTTT
    GGGCCTCTGT TTGATGGAGC AATCGTGAGT GGGAAGCTAC TGCCAAGCCT TATCTGTGCC
    ACCTGCATCA ACGCCAGCCG GGCCGTCAAG TGCCTCATCC CCCTCTACCA GAGCTTCTAC
    GAGGAGAGAG CTCTCTATCT GGAAGCAATA ATTCAGAACC ACCGTGAAGT CATGACATTT
    GAGGATTTCA CAGCCCAGGT CTTCTGTCCA TCTCCCAGCT ACTCGGTCAG CGGAACTGTG
    TCAGCTCTGG AAGAGGGAAG GGATGGATGT GATGAACATC TGCTCCACCT GATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221911
    Clone ID Related Accession (Same CDS sequence) XM_025001042.1
    Accession Version XM_025001042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
    GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA
    TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA
    TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT
    AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG
    TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG
    AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT
    CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC
    CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC
    ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG
    CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG
    CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG
    GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT
    CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA
    CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC
    CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA
    AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT
    CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG
    GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC
    GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC
    ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT
    TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG
    ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA
    GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC
    AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA
    GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG
    GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG
    CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA
    ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA
    ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC
    TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG
    TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG
    GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG
    ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA
    AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG
    TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC
    AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG
    AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG
    CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG
    TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG
    CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC
    TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG
    CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC
    TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT
    CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG
    CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA
    ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA
    ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG
    TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA
    AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT
    GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA
    GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC
    TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC
    CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC
    TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG
    GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC
    TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA
    GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT
    GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA
    CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG
    TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC
    TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG
    GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG
    TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC
    CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA
    CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG
    CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG
    CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC
    AACCTGCTCA CTGTTGAAGA GGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCGCC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG GCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGTGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA
    GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856810.1
    CDS43..5721
    Translation

    Target ORF information:

    RefSeq Version XM_025001042.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
    GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA
    TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA
    TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT
    AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG
    TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG
    AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT
    CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC
    CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC
    ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG
    CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG
    CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG
    GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT
    CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA
    CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC
    CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA
    AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT
    CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG
    GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC
    GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC
    ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT
    TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG
    ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA
    GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC
    AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA
    GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG
    GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG
    CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA
    ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA
    ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC
    TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG
    TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG
    GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG
    ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA
    AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG
    TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC
    AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG
    AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG
    CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG
    TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG
    CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC
    TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG
    CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC
    TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT
    CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG
    CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA
    ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA
    ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG
    TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA
    AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT
    GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA
    GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC
    TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC
    CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC
    TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG
    GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC
    TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA
    GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT
    GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA
    CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG
    TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC
    TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG
    GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG
    TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC
    CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA
    CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG
    CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG
    CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC
    AACCTGCTCA CTGTTGAAGA GGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCGCC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG GCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGTGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA
    GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221912
    Clone ID Related Accession (Same CDS sequence) XM_025001043.1
    Accession Version XM_025001043.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
    AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC
    AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA
    AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG
    GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT
    TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA
    AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT
    CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT
    GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC
    ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG
    GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT
    ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT
    CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA
    ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC
    ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT
    GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA
    CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC
    ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA
    GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC
    ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC
    GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC
    ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT
    GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA
    TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC
    AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG
    GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT
    GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT
    GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT
    AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG
    AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT
    AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC
    TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA
    TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG
    ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA
    GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG
    CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC
    GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA
    GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG
    ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA
    GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG
    AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC
    AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG
    AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG
    CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT
    ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC
    CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC
    ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA
    GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA
    CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT
    TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG
    GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC
    TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC
    CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG
    CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC
    TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA
    GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA
    CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC
    CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT
    GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT
    CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG
    GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG
    TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG
    TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG
    CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC
    ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG
    GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG
    ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG
    AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA
    GAGGCAGAGA GGAGGAGAAG GAGCCTGGGG CTGATCCCGC TGACAGCGCG CATGGTGATG
    GCGCACCTGG TCAACCACCT GGGCCACTTC CCGCTCTGCG GGGGCCCCGC CGTGCTGCAC
    AGCCTGGTGG GCGAGCACCA CGACAACGTG CACGCCGACG CCGCCGAGCT CTCCCCAGAC
    GTGTTCAGGA GCCCCAGCCT GCAGCTGTTT GTGTTCAACG ACAGCACCCT CATCTCCTAC
    CTGCAGACGC CTGCGACCGG TCCTGCCAGT GGGTCGCCAG CGGGCGCCCC CTCGGACGTG
    AGAGTGATCG TGAGGGACAT CTCAGGGAAG TACTCGTGGG ACGGGAAGGT GCTCTACGGA
    CCCCTGGAAG GCCACTTAGC CCCCAAGGGC AGAAGGCCCT CGTTCCTCAT CTCGAGCTGT
    CAACCCCCAA CCCCTGGACC TCAGAGGGCT CCTTCTCAGA CTGAGGAGGG AGAGGATGTC
    CTCGACAAAT TACTTGAAAA CCTTGGCCAC ACGAGTCCTG AATGCCTTTT GCCATCCCAG
    CTCCGTCTGA ATGAGCCTTC CCCACCCCCT TGTGGCATGA ATTGGGACCA GGAGAAGGAG
    ATCACCAAGG TCCTGCTGCG CCAGAGCACG CAAGAAGACG AGTATGTCCA GAGGTGCAAC
    TCGGATGTGG CCATGAGGGT CGCCAGCCAG GAGCCGCCGT CCCCAGTGGA GCCCCGGGGT
    GCCTTCTACT TCTGTAGGCT GCTGCTCGAT GACTTGGGAA TGAATTCTTG GGACAGAAGG
    AAGAACTTCC ATCTGTTGAA GAAAAATTCA AAATTATTGA GAGAGCTAAA AAATCTGGAC
    TCCCGGCAGT GCCGTGAAAC GCACAAAATC GCAGTGTTTT ACATTGCCGA AGGTCAAGAA
    GACAAGTGTT CGATCCTTTC CAATGAAAGG GGAAGCCAAG CGTATGAAGA CTTTGTTGCT
    GGACTTGGAT GGGAGGTGGA CCTGTCAACC CACTGTGGGT TCATGGGCGG GCTTCAGCGC
    AATGGCAGCA CGGGCCAGAC CGCCCCCTAC TACGCTACCT CGACCGTGGA AGTCATCTTC
    CACGTCTCAA CTCGGATGCC GTCAGACTCA GACGACTCGC TCACCAAAAA GCTCCGTCAC
    TTGGGGAATG ACGAAGTCCA CATCGTCTGG TCCGAGCACT CCAGGGACTA CCGCAGCGGG
    ATCATCCCCA CCGCCTTCGG AGACGTTTCA ATCATCATCT ACCCCATGAA GAATCACATG
    TTCTTTATCG CGATAACCAA GAAACCCGAG GTTCCATTTT TTGGGCCTCT GTTTGATGGA
    GCAATCGTGA GTGGGAAGCT ACTGCCAAGC CTTATCTGTG CCACCTGCAT CAACGCCAGC
    CGGGCCGTCA AGTGCCTCAT CCCCCTCTAC CAGAGCTTCT ACGAGGAGAG AGCTCTCTAT
    CTGGAAGCAA TAATTCAGAA CCACCGTGAA GTCATGACAT TTGAGGATTT CACAGCCCAG
    GTCTTCTGTC CATCTCCCAG CTACTCGGTC AGCGGAACTG TGTCAGCTCT GGAAGAGGGA
    AGGGATGGAT GTGATGAACA TCTGCTCCAC CTGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856811.1
    CDS116..5794
    Translation

    Target ORF information:

    RefSeq Version XM_025001043.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
    AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC
    AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA
    AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG
    GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT
    TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA
    AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT
    CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT
    GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC
    ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG
    GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT
    ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT
    CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA
    ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC
    ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT
    GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA
    CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC
    ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA
    GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC
    ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC
    GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC
    ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT
    GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA
    TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC
    AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG
    GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT
    GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT
    GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT
    AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG
    AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT
    AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC
    TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA
    TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG
    ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA
    GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG
    CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC
    GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA
    GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG
    ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA
    GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG
    AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC
    AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG
    AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG
    CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT
    ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC
    CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC
    ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA
    GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA
    CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT
    TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG
    GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC
    TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC
    CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG
    CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC
    TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA
    GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA
    CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC
    CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT
    GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT
    CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG
    GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG
    TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG
    TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG
    CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC
    ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG
    GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG
    ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG
    AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA
    GAGGCAGAGA GGAGGAGAAG GAGCCTGGGG CTGATCCCGC TGACAGCGCG CATGGTGATG
    GCGCACCTGG TCAACCACCT GGGCCACTTC CCGCTCTGCG GGGGCCCCGC CGTGCTGCAC
    AGCCTGGTGG GCGAGCACCA CGACAACGTG CACGCCGACG CCGCCGAGCT CTCCCCAGAC
    GTGTTCAGGA GCCCCAGCCT GCAGCTGTTT GTGTTCAACG ACAGCACCCT CATCTCCTAC
    CTGCAGACGC CTGCGACCGG TCCTGCCAGT GGGTCGCCAG CGGGCGCCCC CTCGGACGTG
    AGAGTGATCG TGAGGGACAT CTCAGGGAAG TACTCGTGGG ACGGGAAGGT GCTCTACGGA
    CCCCTGGAAG GCCACTTAGC CCCCAAGGGC AGAAGGCCCT CGTTCCTCAT CTCGAGCTGT
    CAACCCCCAA CCCCTGGACC TCAGAGGGCT CCTTCTCAGA CTGAGGAGGG AGAGGATGTC
    CTCGACAAAT TACTTGAAAA CCTTGGCCAC ACGAGTCCTG AATGCCTTTT GCCATCCCAG
    CTCCGTCTGA ATGAGCCTTC CCCACCCCCT TGTGGCATGA ATTGGGACCA GGAGAAGGAG
    ATCACCAAGG TCCTGCTGCG CCAGAGCACG CAAGAAGACG AGTATGTCCA GAGGTGCAAC
    TCGGATGTGG CCATGAGGGT CGCCAGCCAG GAGCCGCCGT CCCCAGTGGA GCCCCGGGGT
    GCCTTCTACT TCTGTAGGCT GCTGCTCGAT GACTTGGGAA TGAATTCTTG GGACAGAAGG
    AAGAACTTCC ATCTGTTGAA GAAAAATTCA AAATTATTGA GAGAGCTAAA AAATCTGGAC
    TCCCGGCAGT GCCGTGAAAC GCACAAAATC GCAGTGTTTT ACATTGCCGA AGGTCAAGAA
    GACAAGTGTT CGATCCTTTC CAATGAAAGG GGAAGCCAAG CGTATGAAGA CTTTGTTGCT
    GGACTTGGAT GGGAGGTGGA CCTGTCAACC CACTGTGGGT TCATGGGCGG GCTTCAGCGC
    AATGGCAGCA CGGGCCAGAC CGCCCCCTAC TACGCTACCT CGACCGTGGA AGTCATCTTC
    CACGTCTCAA CTCGGATGCC GTCAGACTCA GACGACTCGC TCACCAAAAA GCTCCGTCAC
    TTGGGGAATG ACGAAGTCCA CATCGTCTGG TCCGAGCACT CCAGGGACTA CCGCAGCGGG
    ATCATCCCCA CCGCCTTCGG AGACGTTTCA ATCATCATCT ACCCCATGAA GAATCACATG
    TTCTTTATCG CGATAACCAA GAAACCCGAG GTTCCATTTT TTGGGCCTCT GTTTGATGGA
    GCAATCGTGA GTGGGAAGCT ACTGCCAAGC CTTATCTGTG CCACCTGCAT CAACGCCAGC
    CGGGCCGTCA AGTGCCTCAT CCCCCTCTAC CAGAGCTTCT ACGAGGAGAG AGCTCTCTAT
    CTGGAAGCAA TAATTCAGAA CCACCGTGAA GTCATGACAT TTGAGGATTT CACAGCCCAG
    GTCTTCTGTC CATCTCCCAG CTACTCGGTC AGCGGAACTG TGTCAGCTCT GGAAGAGGGA
    AGGGATGGAT GTGATGAACA TCTGCTCCAC CTGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221913
    Clone ID Related Accession (Same CDS sequence) XM_025001044.1
    Accession Version XM_025001044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
    AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC
    AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA
    AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG
    GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT
    TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA
    AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT
    CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT
    GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC
    ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG
    GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT
    ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT
    CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA
    ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC
    ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT
    GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA
    CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC
    ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA
    GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC
    ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC
    GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC
    ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT
    GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA
    TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC
    AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG
    GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT
    GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT
    GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT
    AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG
    AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT
    AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC
    TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA
    TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG
    ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA
    GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG
    CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC
    GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA
    GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG
    ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA
    GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG
    AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC
    AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG
    AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG
    CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT
    ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC
    CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC
    ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA
    GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA
    CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT
    TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG
    GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC
    TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC
    CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG
    CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC
    TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA
    GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA
    CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC
    CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT
    GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT
    CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG
    GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG
    TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG
    TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG
    CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC
    ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG
    GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG
    ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG
    AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA
    GAGGAGAGGA GGAGAAGGAG CCTGGGGCTG ATCCCGCTGA CAGCGCGCAT GGTGATGGCG
    CACCTGGTCA ACCACCTGGG CCACTTCCCG CTCTGCGGGG GCCCCGCCGT GCTGCACAGC
    CTGGTGGGCG AGCACCACGA CAACGTGCAC GCCGACGCCG CCGAGCTCTC CCCAGACGTG
    TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG TTCAACGACA GCACCCTCAT CTCCTACCTG
    CAGACGCCTG CGACCGGTCC TGCCAGTGGG TCGCCAGCGG GCGCCCCCTC GGACGTGAGA
    GTGATCGTGA GGGACATCTC AGGGAAGTAC TCGTGGGACG GGAAGGTGCT CTACGGACCC
    CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA AGGCCCTCGT TCCTCATCTC GAGCTGTCAA
    CCCCCAACCC CTGGACCTCA GAGGGCTCCT TCTCAGACTG AGGAGGGAGA GGATGTCCTC
    GACAAATTAC TTGAAAACCT TGGCCACACG AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC
    CGTCTGAATG AGCCTTCCCC ACCCCCTTGT GGCATGAATT GGGACCAGGA GAAGGAGATC
    ACCAAGGTCC TGCTGCGCCA GAGCACGCAA GAAGACGAGT ATGTCCAGAG GTGCAACTCG
    GATGTGGCCA TGAGGGTCGC CAGCCAGGAG CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC
    TTCTACTTCT GTAGGCTGCT GCTCGATGAC TTGGGAATGA ATTCTTGGGA CAGAAGGAAG
    AACTTCCATC TGTTGAAGAA AAATTCAAAA TTATTGAGAG AGCTAAAAAA TCTGGACTCC
    CGGCAGTGCC GTGAAACGCA CAAAATCGCA GTGTTTTACA TTGCCGAAGG TCAAGAAGAC
    AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA AGCCAAGCGT ATGAAGACTT TGTTGCTGGA
    CTTGGATGGG AGGTGGACCT GTCAACCCAC TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT
    GGCAGCACGG GCCAGACCGC CCCCTACTAC GCTACCTCGA CCGTGGAAGT CATCTTCCAC
    GTCTCAACTC GGATGCCGTC AGACTCAGAC GACTCGCTCA CCAAAAAGCT CCGTCACTTG
    GGGAATGACG AAGTCCACAT CGTCTGGTCC GAGCACTCCA GGGACTACCG CAGCGGGATC
    ATCCCCACCG CCTTCGGAGA CGTTTCAATC ATCATCTACC CCATGAAGAA TCACATGTTC
    TTTATCGCGA TAACCAAGAA ACCCGAGGTT CCATTTTTTG GGCCTCTGTT TGATGGAGCA
    ATCGTGAGTG GGAAGCTACT GCCAAGCCTT ATCTGTGCCA CCTGCATCAA CGCCAGCCGG
    GCCGTCAAGT GCCTCATCCC CCTCTACCAG AGCTTCTACG AGGAGAGAGC TCTCTATCTG
    GAAGCAATAA TTCAGAACCA CCGTGAAGTC ATGACATTTG AGGATTTCAC AGCCCAGGTC
    TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC GGAACTGTGT CAGCTCTGGA AGAGGGAAGG
    GATGGATGTG ATGAACATCT GCTCCACCTG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856812.1
    CDS1..5676
    Translation

    Target ORF information:

    RefSeq Version XM_025001044.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
    AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC
    AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA
    AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG
    GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT
    TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA
    AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT
    CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT
    GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC
    ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG
    GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT
    ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT
    CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA
    ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC
    ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT
    GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA
    CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC
    ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA
    GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC
    ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC
    GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC
    ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT
    GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA
    TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC
    AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG
    GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT
    GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT
    GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT
    AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG
    AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT
    AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC
    TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA
    TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG
    ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA
    GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG
    CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC
    GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA
    GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG
    ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA
    GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG
    AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC
    AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG
    AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG
    CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT
    ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC
    CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC
    ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA
    GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA
    CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT
    TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG
    GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC
    TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC
    CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG
    CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC
    TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA
    GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA
    CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC
    CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT
    GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT
    CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG
    GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG
    TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG
    TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG
    CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC
    ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG
    GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG
    ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG
    AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA
    GAGGAGAGGA GGAGAAGGAG CCTGGGGCTG ATCCCGCTGA CAGCGCGCAT GGTGATGGCG
    CACCTGGTCA ACCACCTGGG CCACTTCCCG CTCTGCGGGG GCCCCGCCGT GCTGCACAGC
    CTGGTGGGCG AGCACCACGA CAACGTGCAC GCCGACGCCG CCGAGCTCTC CCCAGACGTG
    TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG TTCAACGACA GCACCCTCAT CTCCTACCTG
    CAGACGCCTG CGACCGGTCC TGCCAGTGGG TCGCCAGCGG GCGCCCCCTC GGACGTGAGA
    GTGATCGTGA GGGACATCTC AGGGAAGTAC TCGTGGGACG GGAAGGTGCT CTACGGACCC
    CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA AGGCCCTCGT TCCTCATCTC GAGCTGTCAA
    CCCCCAACCC CTGGACCTCA GAGGGCTCCT TCTCAGACTG AGGAGGGAGA GGATGTCCTC
    GACAAATTAC TTGAAAACCT TGGCCACACG AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC
    CGTCTGAATG AGCCTTCCCC ACCCCCTTGT GGCATGAATT GGGACCAGGA GAAGGAGATC
    ACCAAGGTCC TGCTGCGCCA GAGCACGCAA GAAGACGAGT ATGTCCAGAG GTGCAACTCG
    GATGTGGCCA TGAGGGTCGC CAGCCAGGAG CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC
    TTCTACTTCT GTAGGCTGCT GCTCGATGAC TTGGGAATGA ATTCTTGGGA CAGAAGGAAG
    AACTTCCATC TGTTGAAGAA AAATTCAAAA TTATTGAGAG AGCTAAAAAA TCTGGACTCC
    CGGCAGTGCC GTGAAACGCA CAAAATCGCA GTGTTTTACA TTGCCGAAGG TCAAGAAGAC
    AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA AGCCAAGCGT ATGAAGACTT TGTTGCTGGA
    CTTGGATGGG AGGTGGACCT GTCAACCCAC TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT
    GGCAGCACGG GCCAGACCGC CCCCTACTAC GCTACCTCGA CCGTGGAAGT CATCTTCCAC
    GTCTCAACTC GGATGCCGTC AGACTCAGAC GACTCGCTCA CCAAAAAGCT CCGTCACTTG
    GGGAATGACG AAGTCCACAT CGTCTGGTCC GAGCACTCCA GGGACTACCG CAGCGGGATC
    ATCCCCACCG CCTTCGGAGA CGTTTCAATC ATCATCTACC CCATGAAGAA TCACATGTTC
    TTTATCGCGA TAACCAAGAA ACCCGAGGTT CCATTTTTTG GGCCTCTGTT TGATGGAGCA
    ATCGTGAGTG GGAAGCTACT GCCAAGCCTT ATCTGTGCCA CCTGCATCAA CGCCAGCCGG
    GCCGTCAAGT GCCTCATCCC CCTCTACCAG AGCTTCTACG AGGAGAGAGC TCTCTATCTG
    GAAGCAATAA TTCAGAACCA CCGTGAAGTC ATGACATTTG AGGATTTCAC AGCCCAGGTC
    TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC GGAACTGTGT CAGCTCTGGA AGAGGGAAGG
    GATGGATGTG ATGAACATCT GCTCCACCTG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221914
    Clone ID Related Accession (Same CDS sequence) XM_025001045.1
    Accession Version XM_025001045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
    GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA
    TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA
    TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT
    AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG
    TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG
    AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT
    CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC
    CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC
    ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG
    CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG
    CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG
    GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT
    CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA
    CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC
    CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA
    AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT
    CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG
    GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC
    GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC
    ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT
    TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG
    ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA
    GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC
    AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA
    GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG
    GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG
    CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA
    ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA
    ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC
    TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG
    TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG
    GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG
    ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA
    AAGAAACAGC GAGGCAAAGA AGTGGAAGAA TACCTGCAGC CAGAAAACAC ACCTGCCACC
    GAATCTGGGC CCCTGGGGCA GCAGCCGATG GTTCGGAGCG GCAGCAGCCC TGAGGTCACC
    GAGCCGCTGT GTCCTGAGCC CCCCCCAGGC CAAAAGCTAG AAAATGCACA GAACGTGAGT
    TCTTCAGAGC CCAGACTTGC TCAGGAGAGC AAAGGACATG AGAAGAAAGA GCATGAAGGA
    ATAACAATCT TAGTTCGAAG AAGCAGCAGC CCCATTGAAC TGGATTTGAA AGATGACTCA
    CAGCAGATGC AAGGAAAACG TAGGGAGAGA CAGAAGAGTG ACAGTGTGAG CAGTGACCCC
    ACTCTGGGCT GCAGCACTGA GGCAGAGCTG TCCCTGAGCC ACTGGCAGAC CTGCGAGGAA
    GACCCAGATC TGAGCCCGCC TGCAGATACT GTGACCGACG CTGACGCCCG CCACTGGTTA
    CAGCTCCGGC CCACGGATGC TTCAAACCTA ACAGACAGCA GCGAATGCCT TGCTGATGAC
    TGCAGTATAA TTGCTGGGGG GACCCTCACT GGTTGGCACC CAGACTCAGC CGCTGTGTTG
    TGGCGAAGAA TCTTGGGAAT CCTTGGAGAT GTGAATAATA TCCAGTCACC CAAAATCCAT
    GCCAGGGTGT TTGGCTATCT CTATGAACTC TGGTACAAGC TAGCAAAGAT ACGGGATAAC
    CTGGCAATAA GCCTGGATAA CCAGTCTTCT CCATCTCCTC CTGCCTTGAT CCCACCGCTC
    AGAATGTTTG CATCATGGCT ATTTAAGGCG ACGACGATGC CTGACGATTA TAAGGAAGGC
    AAACTGCAAG CCTATAAGCT GATCTGCTCC ATGATGACCC GGCGCCAGGA CGTCCTGCCC
    AACTCAGACT TCCTGGTGCA TTTTTACCTC GTCATGCACC TGGGACTGAC CAGCGAGGAC
    CAGGATATCC TGAATACCAT CATAAGGCAC TGCCCGCCCC GCTTCTTCTC CCTGGGCTTG
    CCTGGCTTCT CCATGCTGGT GGGGGACTTC ATCATGGCTG CTGGCAGGGT CCTCAGTGCA
    GACACGCTGG CGGCACCTCG TTCGGAAGCC CTCACCATTC TCGGGTCTCT GGTCTGCTTT
    CCGAACACCT ACCGCGAGAT TCCCCTACGG CAGCCGGTAC CTGAAGTTGC TGAAGTCGCC
    ATGGGAGCAG AAGATGTCAA GCACTGCCTC ATAAGTATCT TACTGAAGAA TGCCACAGAG
    GAACCGAGTG AGTGTGCAAG GTGCATTGCC ATCTGCTCCC TCGGGATCTG GATCTGTGAG
    GAGCTGGCAC AAGGCACTGG CCACCCTCAG CTGCAAGAGG CCGTCAACGT CATTGGTGTC
    ACGTTGAAGT TTCCTAACAA AATTGTGGCC CAGGTAGCCT GTGACGTGCT TCAGCTGCTA
    GTCTCCTACT GGGAAAAGCT TCAGATGTTT GAAACCTCCT TGCCTCGGAA AATTGCAGAA
    ATCCTTGTGG CCACAATTGC TTTTCTGTTA CCAAATGCAG AGTACTCCTC GGTGGAGACA
    GACAAGAAGT TCCTCGTGTC CTTGTTGCTC TGCCTCCTGG ACTGGTGCAT GGCGCTGCCG
    GCAGGAACCC TCCTCCACCC CGTGTCCACG GCGGCCCTGG ACGAACAGGC CCCGGCCCGG
    GCCCCCTTAC TGGATTACAT CTACAGGGTT CTGCACTGCT GCATCTGTGG CTCAAGCACG
    TACAGCCAGC AGAGCCGCTA CACCTTGACC CTGGCGGACC TGTCGTCCTC GGATTACGAC
    CCCTTCCTGC CGCTGGCGAA CGTGAAGAAT TCTGAGCCGG TCCAATTTCA TTCATCAGCA
    GAACTGGGCA ACCTGCTCAC TGTTGAAGAG GCAGAGAGGA GGAGAAGGAG CCTGGGGCTG
    ATCCCGCTGA CAGCGCGCAT GGTGATGGCG CACCTGGTCA ACCACCTGGG CCACTTCCCG
    CTCTGCGGGG GCCCCGCCGT GCTGCACAGC CTGGTGGGCG AGCACCACGA CAACGTGCAC
    GCCGACGCCG CCGAGCTCTC CCCAGACGTG TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG
    TTCAACGACA GCACCCTCAT CTCCTACCTG CAGACGCCTG CGACCGGTCC TGCCAGTGGG
    TCGCCAGCGG GCGCCCCCTC GGACGTGAGA GTGATCGTGA GGGACATCTC AGGGAAGTAC
    TCGTGGGACG GGAAGGTGCT CTACGGACCC CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA
    AGGCCCTCGT TCCTCATCTC GAGCTGTCAA CCCCCAACCC CTGGACCTCA GAGGGCTCCT
    TCTCAGACTG AGGAGGGAGA GGATGTCCTC GACAAATTAC TTGAAAACCT TGGCCACACG
    AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC CGTCTGAATG AGCCTTCCCC ACCCCCTTGT
    GGCATGAATT GGGACCAGGA GAAGGAGATC ACCAAGGTCC TGCTGCGCCA GAGCACGCAA
    GAAGACGAGT ATGTCCAGAG GTGCAACTCG GATGTGGCCA TGAGGGTCGC CAGCCAGGAG
    CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC TTCTACTTCT GTAGGCTGCT GCTCGATGAC
    TTGGGAATGA ATTCTTGGGA CAGAAGGAAG AACTTCCATC TGTTGAAGAA AAATTCAAAA
    TTATTGAGAG AGCTAAAAAA TCTGGACTCC CGGCAGTGCC GTGAAACGCA CAAAATCGCA
    GTGTTTTACA TTGCCGAAGG TCAAGAAGAC AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA
    AGCCAAGCGT ATGAAGACTT TGTTGCTGGA CTTGGATGGG AGGTGGACCT GTCAACCCAC
    TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT GGCAGCACGG GCCAGACCGC CCCCTACTAC
    GCTACCTCGA CCGTGGAAGT CATCTTCCAC GTCTCAACTC GGATGCCGTC AGACTCAGAC
    GACTCGCTCA CCAAAAAGCT CCGTCACTTG GGGAATGACG AAGTCCACAT CGTCTGGTCC
    GAGCACTCCA GGGACTACCG CAGCGGGATC ATCCCCACCG CCTTCGGAGA CGTTTCAATC
    ATCATCTACC CCATGAAGAA TCACATGTTC TTTATCGCGA TAACCAAGAA ACCCGAGGTT
    CCATTTTTTG GGCCTCTGTT TGATGGAGCA ATCGTGAGTG GGAAGCTACT GCCAAGCCTT
    ATCTGTGCCA CCTGCATCAA CGCCAGCCGG GCCGTCAAGT GCCTCATCCC CCTCTACCAG
    AGCTTCTACG AGGAGAGAGC TCTCTATCTG GAAGCAATAA TTCAGAACCA CCGTGAAGTC
    ATGACATTTG AGGATTTCAC AGCCCAGGTC TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC
    GGAACTGTGT CAGCTCTGGA AGAGGGAAGG GATGGATGTG ATGAACATCT GCTCCACCTG
    ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856813.1
    CDS43..5568
    Translation

    Target ORF information:

    RefSeq Version XM_025001045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
    GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA
    TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA
    TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT
    AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG
    TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG
    AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT
    CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC
    CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC
    ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG
    CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG
    CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG
    GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT
    CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA
    CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC
    CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA
    AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT
    CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG
    GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC
    GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC
    ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT
    TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG
    ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA
    GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC
    AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA
    GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG
    GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG
    CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA
    ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA
    ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC
    TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG
    TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG
    GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG
    ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA
    AAGAAACAGC GAGGCAAAGA AGTGGAAGAA TACCTGCAGC CAGAAAACAC ACCTGCCACC
    GAATCTGGGC CCCTGGGGCA GCAGCCGATG GTTCGGAGCG GCAGCAGCCC TGAGGTCACC
    GAGCCGCTGT GTCCTGAGCC CCCCCCAGGC CAAAAGCTAG AAAATGCACA GAACGTGAGT
    TCTTCAGAGC CCAGACTTGC TCAGGAGAGC AAAGGACATG AGAAGAAAGA GCATGAAGGA
    ATAACAATCT TAGTTCGAAG AAGCAGCAGC CCCATTGAAC TGGATTTGAA AGATGACTCA
    CAGCAGATGC AAGGAAAACG TAGGGAGAGA CAGAAGAGTG ACAGTGTGAG CAGTGACCCC
    ACTCTGGGCT GCAGCACTGA GGCAGAGCTG TCCCTGAGCC ACTGGCAGAC CTGCGAGGAA
    GACCCAGATC TGAGCCCGCC TGCAGATACT GTGACCGACG CTGACGCCCG CCACTGGTTA
    CAGCTCCGGC CCACGGATGC TTCAAACCTA ACAGACAGCA GCGAATGCCT TGCTGATGAC
    TGCAGTATAA TTGCTGGGGG GACCCTCACT GGTTGGCACC CAGACTCAGC CGCTGTGTTG
    TGGCGAAGAA TCTTGGGAAT CCTTGGAGAT GTGAATAATA TCCAGTCACC CAAAATCCAT
    GCCAGGGTGT TTGGCTATCT CTATGAACTC TGGTACAAGC TAGCAAAGAT ACGGGATAAC
    CTGGCAATAA GCCTGGATAA CCAGTCTTCT CCATCTCCTC CTGCCTTGAT CCCACCGCTC
    AGAATGTTTG CATCATGGCT ATTTAAGGCG ACGACGATGC CTGACGATTA TAAGGAAGGC
    AAACTGCAAG CCTATAAGCT GATCTGCTCC ATGATGACCC GGCGCCAGGA CGTCCTGCCC
    AACTCAGACT TCCTGGTGCA TTTTTACCTC GTCATGCACC TGGGACTGAC CAGCGAGGAC
    CAGGATATCC TGAATACCAT CATAAGGCAC TGCCCGCCCC GCTTCTTCTC CCTGGGCTTG
    CCTGGCTTCT CCATGCTGGT GGGGGACTTC ATCATGGCTG CTGGCAGGGT CCTCAGTGCA
    GACACGCTGG CGGCACCTCG TTCGGAAGCC CTCACCATTC TCGGGTCTCT GGTCTGCTTT
    CCGAACACCT ACCGCGAGAT TCCCCTACGG CAGCCGGTAC CTGAAGTTGC TGAAGTCGCC
    ATGGGAGCAG AAGATGTCAA GCACTGCCTC ATAAGTATCT TACTGAAGAA TGCCACAGAG
    GAACCGAGTG AGTGTGCAAG GTGCATTGCC ATCTGCTCCC TCGGGATCTG GATCTGTGAG
    GAGCTGGCAC AAGGCACTGG CCACCCTCAG CTGCAAGAGG CCGTCAACGT CATTGGTGTC
    ACGTTGAAGT TTCCTAACAA AATTGTGGCC CAGGTAGCCT GTGACGTGCT TCAGCTGCTA
    GTCTCCTACT GGGAAAAGCT TCAGATGTTT GAAACCTCCT TGCCTCGGAA AATTGCAGAA
    ATCCTTGTGG CCACAATTGC TTTTCTGTTA CCAAATGCAG AGTACTCCTC GGTGGAGACA
    GACAAGAAGT TCCTCGTGTC CTTGTTGCTC TGCCTCCTGG ACTGGTGCAT GGCGCTGCCG
    GCAGGAACCC TCCTCCACCC CGTGTCCACG GCGGCCCTGG ACGAACAGGC CCCGGCCCGG
    GCCCCCTTAC TGGATTACAT CTACAGGGTT CTGCACTGCT GCATCTGTGG CTCAAGCACG
    TACAGCCAGC AGAGCCGCTA CACCTTGACC CTGGCGGACC TGTCGTCCTC GGATTACGAC
    CCCTTCCTGC CGCTGGCGAA CGTGAAGAAT TCTGAGCCGG TCCAATTTCA TTCATCAGCA
    GAACTGGGCA ACCTGCTCAC TGTTGAAGAG GCAGAGAGGA GGAGAAGGAG CCTGGGGCTG
    ATCCCGCTGA CAGCGCGCAT GGTGATGGCG CACCTGGTCA ACCACCTGGG CCACTTCCCG
    CTCTGCGGGG GCCCCGCCGT GCTGCACAGC CTGGTGGGCG AGCACCACGA CAACGTGCAC
    GCCGACGCCG CCGAGCTCTC CCCAGACGTG TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG
    TTCAACGACA GCACCCTCAT CTCCTACCTG CAGACGCCTG CGACCGGTCC TGCCAGTGGG
    TCGCCAGCGG GCGCCCCCTC GGACGTGAGA GTGATCGTGA GGGACATCTC AGGGAAGTAC
    TCGTGGGACG GGAAGGTGCT CTACGGACCC CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA
    AGGCCCTCGT TCCTCATCTC GAGCTGTCAA CCCCCAACCC CTGGACCTCA GAGGGCTCCT
    TCTCAGACTG AGGAGGGAGA GGATGTCCTC GACAAATTAC TTGAAAACCT TGGCCACACG
    AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC CGTCTGAATG AGCCTTCCCC ACCCCCTTGT
    GGCATGAATT GGGACCAGGA GAAGGAGATC ACCAAGGTCC TGCTGCGCCA GAGCACGCAA
    GAAGACGAGT ATGTCCAGAG GTGCAACTCG GATGTGGCCA TGAGGGTCGC CAGCCAGGAG
    CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC TTCTACTTCT GTAGGCTGCT GCTCGATGAC
    TTGGGAATGA ATTCTTGGGA CAGAAGGAAG AACTTCCATC TGTTGAAGAA AAATTCAAAA
    TTATTGAGAG AGCTAAAAAA TCTGGACTCC CGGCAGTGCC GTGAAACGCA CAAAATCGCA
    GTGTTTTACA TTGCCGAAGG TCAAGAAGAC AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA
    AGCCAAGCGT ATGAAGACTT TGTTGCTGGA CTTGGATGGG AGGTGGACCT GTCAACCCAC
    TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT GGCAGCACGG GCCAGACCGC CCCCTACTAC
    GCTACCTCGA CCGTGGAAGT CATCTTCCAC GTCTCAACTC GGATGCCGTC AGACTCAGAC
    GACTCGCTCA CCAAAAAGCT CCGTCACTTG GGGAATGACG AAGTCCACAT CGTCTGGTCC
    GAGCACTCCA GGGACTACCG CAGCGGGATC ATCCCCACCG CCTTCGGAGA CGTTTCAATC
    ATCATCTACC CCATGAAGAA TCACATGTTC TTTATCGCGA TAACCAAGAA ACCCGAGGTT
    CCATTTTTTG GGCCTCTGTT TGATGGAGCA ATCGTGAGTG GGAAGCTACT GCCAAGCCTT
    ATCTGTGCCA CCTGCATCAA CGCCAGCCGG GCCGTCAAGT GCCTCATCCC CCTCTACCAG
    AGCTTCTACG AGGAGAGAGC TCTCTATCTG GAAGCAATAA TTCAGAACCA CCGTGAAGTC
    ATGACATTTG AGGATTTCAC AGCCCAGGTC TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC
    GGAACTGTGT CAGCTCTGGA AGAGGGAAGG GATGGATGTG ATGAACATCT GCTCCACCTG
    ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92268
    Clone ID Related Accession (Same CDS sequence) XM_015474043.1
    Accession Version XM_015474043.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGTGTC AGCTCTGGAA
    GAGGGAAGAG ATGGATGTGA TGAACATCTG CTCCACCTGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329529.1
    CDS16..5760
    Translation

    Target ORF information:

    RefSeq Version XM_015474043.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGTGTC AGCTCTGGAA
    GAGGGAAGAG ATGGATGTGA TGAACATCTG CTCCACCTGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92269
    Clone ID Related Accession (Same CDS sequence) XM_015474044.1
    Accession Version XM_015474044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG AGAGGAGGAG AAGGAGCCTG GGGCTGATCC CGCTGACAGC GCGCATGGTG
    ATGGCGCACC TGGTCAACCA CCTGGGCCAC TTCCCGCTCT GCGGGGGCCC CGCCGTGCTG
    CACAGCCTGG TGGGCGAGCA CCACGACAAC GTGCACGCCG ACGCCACCGA GCTCTCCCCA
    GACGTGTTCA GGAGCCCCAG CCTGCAGCTG TTTGTGTTCA ACGACAGCAC CCTCATCTCC
    TACCTGCAGA CGCCTGCGAC CGGTCCTGCC AGTGGGTCGC CAGCGGGCGC CCCCTCGGAC
    GTGAGAGTGA TCGTGAGGGA CATCTCAGGG AAGTACTCGT GGGACGGGAA GGTGCTCTAC
    GGACCCCTGG AAGGCCACTT AGCCCCCAAG GGCAGAAGCC CCTCGTTCCT CATCTCGAGC
    TGTCAACCCC CAACCCCTGG ACCTCAGAGG GCTCCTTCTC AGACTGAGGA GGGAGAGGAT
    GTCCTCGACA AATTACTTGA AAACCTTGGC CACACGAGTC CTGAATGCCT TTTGCCATCC
    CAGCTCCGTC TGAATGAGCC TTCCCCACCC CCTTGTGGCA TGAATTGGGA CCAGGAGAAG
    GAGATCACCA AGGTCCTGCT GCGCCAGAGC ACGCAAGAAG ACGAGTATGT CCAGAGGTGC
    AACTCGGATG TGGCCATGAG GGTCGCCAGC CAGGAGCCGC CGTCCCCAGT GGAGCCCCGG
    GGCGCCTTCT ACTTCTGTAG GCTGCTGCTC GATGACTTGG GAATGAATTC TTGGGACAGA
    AGGAAGAACT TCCATCTGTT GAAGAAAAAT TCAAAATTAT TGAGAGAGCT AAAAAATCTG
    GACTCCCGGC AGTGCCGTGA AACGCACAAA ATCGCAGTGT TTTACATTGC CGAAGGTCAA
    GAAGACAAGT GTTCGATCCT TTCCAATGAA AGGGGAAGCC AAGCGTATGA AGACTTTGTT
    GCTGGACTTG GATGGGAGGT GGACCTGTCA ACCCACTGTG GGTTCATGGG CGGGCTTCAG
    CGCAATGGCA GCACGGGCCA GACCGCCCCC TACTACGCTA CCTCGACCGT GGAAGTCATC
    TTCCACGTCT CAACTCGGAT GCCGTCAGAC TCAGACGACT CGCTCACCAA AAAGCTCCGT
    CACTTGGGGA ATGACGAAGT CCACATCGTC TGGTCCGAGC ACTCCAGGGA CTACCGCAGC
    GGGATCATCC CCACCGCCTT CGGAGACGTT TCAATCATCA TCTACCCCAT GAAGAATCAC
    ATGTTCTTTA TCGCGATAAC CAAGAAACCC GAGGTTCCAT TTTTTGGGCC TCTGTTTGAT
    GGAGCAATCG TGAGTGGGAA GCTACTGCCA AGCCTTATCT GTGCCACCTG CATCAACGCC
    AGCCGGGCCG TCAAGTGCCT CATCCCCCTC TACCAGAGCT TCTACGAGGA GAGAGCTCTC
    TATCTGGAAG CAATAATTCA GAACCACCGT GAAGTCATGA CATTTGAGGA TTTCACAGCC
    CAGGTCTTCT GTCCATCTCC CAGCTACTCG GTCAGCGGAA CTGTGTCAGC TCTGGAAGAG
    GGAAGAGATG GATGTGATGA ACATCTGCTC CACCTGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329530.1
    CDS16..5757
    Translation

    Target ORF information:

    RefSeq Version XM_015474044.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG AGAGGAGGAG AAGGAGCCTG GGGCTGATCC CGCTGACAGC GCGCATGGTG
    ATGGCGCACC TGGTCAACCA CCTGGGCCAC TTCCCGCTCT GCGGGGGCCC CGCCGTGCTG
    CACAGCCTGG TGGGCGAGCA CCACGACAAC GTGCACGCCG ACGCCACCGA GCTCTCCCCA
    GACGTGTTCA GGAGCCCCAG CCTGCAGCTG TTTGTGTTCA ACGACAGCAC CCTCATCTCC
    TACCTGCAGA CGCCTGCGAC CGGTCCTGCC AGTGGGTCGC CAGCGGGCGC CCCCTCGGAC
    GTGAGAGTGA TCGTGAGGGA CATCTCAGGG AAGTACTCGT GGGACGGGAA GGTGCTCTAC
    GGACCCCTGG AAGGCCACTT AGCCCCCAAG GGCAGAAGCC CCTCGTTCCT CATCTCGAGC
    TGTCAACCCC CAACCCCTGG ACCTCAGAGG GCTCCTTCTC AGACTGAGGA GGGAGAGGAT
    GTCCTCGACA AATTACTTGA AAACCTTGGC CACACGAGTC CTGAATGCCT TTTGCCATCC
    CAGCTCCGTC TGAATGAGCC TTCCCCACCC CCTTGTGGCA TGAATTGGGA CCAGGAGAAG
    GAGATCACCA AGGTCCTGCT GCGCCAGAGC ACGCAAGAAG ACGAGTATGT CCAGAGGTGC
    AACTCGGATG TGGCCATGAG GGTCGCCAGC CAGGAGCCGC CGTCCCCAGT GGAGCCCCGG
    GGCGCCTTCT ACTTCTGTAG GCTGCTGCTC GATGACTTGG GAATGAATTC TTGGGACAGA
    AGGAAGAACT TCCATCTGTT GAAGAAAAAT TCAAAATTAT TGAGAGAGCT AAAAAATCTG
    GACTCCCGGC AGTGCCGTGA AACGCACAAA ATCGCAGTGT TTTACATTGC CGAAGGTCAA
    GAAGACAAGT GTTCGATCCT TTCCAATGAA AGGGGAAGCC AAGCGTATGA AGACTTTGTT
    GCTGGACTTG GATGGGAGGT GGACCTGTCA ACCCACTGTG GGTTCATGGG CGGGCTTCAG
    CGCAATGGCA GCACGGGCCA GACCGCCCCC TACTACGCTA CCTCGACCGT GGAAGTCATC
    TTCCACGTCT CAACTCGGAT GCCGTCAGAC TCAGACGACT CGCTCACCAA AAAGCTCCGT
    CACTTGGGGA ATGACGAAGT CCACATCGTC TGGTCCGAGC ACTCCAGGGA CTACCGCAGC
    GGGATCATCC CCACCGCCTT CGGAGACGTT TCAATCATCA TCTACCCCAT GAAGAATCAC
    ATGTTCTTTA TCGCGATAAC CAAGAAACCC GAGGTTCCAT TTTTTGGGCC TCTGTTTGAT
    GGAGCAATCG TGAGTGGGAA GCTACTGCCA AGCCTTATCT GTGCCACCTG CATCAACGCC
    AGCCGGGCCG TCAAGTGCCT CATCCCCCTC TACCAGAGCT TCTACGAGGA GAGAGCTCTC
    TATCTGGAAG CAATAATTCA GAACCACCGT GAAGTCATGA CATTTGAGGA TTTCACAGCC
    CAGGTCTTCT GTCCATCTCC CAGCTACTCG GTCAGCGGAA CTGTGTCAGC TCTGGAAGAG
    GGAAGAGATG GATGTGATGA ACATCTGCTC CACCTGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329536.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329532.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474046.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329533.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474047.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474052.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329538.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474052.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329534.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474048.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329537.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474051.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474053.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329539.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474053.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329531.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92270
    Clone ID Related Accession (Same CDS sequence) XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
    Accession Version XM_015474049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329535.1
    CDS16..5745
    Translation

    Target ORF information:

    RefSeq Version XM_015474049.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA
    AACCCAGACG AGCCAGGCAG CTTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92271
    Clone ID Related Accession (Same CDS sequence) XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
    Accession Version XM_015474056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329542.1
    CDS16..5712
    Translation

    Target ORF information:

    RefSeq Version XM_015474056.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92271
    Clone ID Related Accession (Same CDS sequence) XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
    Accession Version XM_015474055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329541.1
    CDS16..5712
    Translation

    Target ORF information:

    RefSeq Version XM_015474055.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92271
    Clone ID Related Accession (Same CDS sequence) XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
    Accession Version XM_015474054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329540.1
    CDS16..5712
    Translation

    Target ORF information:

    RefSeq Version XM_015474054.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92271
    Clone ID Related Accession (Same CDS sequence) XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
    Accession Version XM_015474057.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329543.1
    CDS16..5712
    Translation

    Target ORF information:

    RefSeq Version XM_015474057.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92272
    Clone ID Related Accession (Same CDS sequence) XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
    Accession Version XM_015474060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329546.1
    CDS16..5706
    Translation

    Target ORF information:

    RefSeq Version XM_015474060.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92272
    Clone ID Related Accession (Same CDS sequence) XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
    Accession Version XM_015474061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329547.1
    CDS16..5706
    Translation

    Target ORF information:

    RefSeq Version XM_015474061.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92272
    Clone ID Related Accession (Same CDS sequence) XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
    Accession Version XM_015474058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329544.1
    CDS16..5706
    Translation

    Target ORF information:

    RefSeq Version XM_015474058.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92272
    Clone ID Related Accession (Same CDS sequence) XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
    Accession Version XM_015474059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329545.1
    CDS16..5706
    Translation

    Target ORF information:

    RefSeq Version XM_015474059.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC
    AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC
    ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA
    GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG
    CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC
    CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT
    CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA
    AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT
    AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG
    GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT
    GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT
    TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG
    ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC
    CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC
    TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC
    CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA
    TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG
    ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT
    TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC
    ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG
    GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT
    TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT
    CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG
    CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG
    TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC
    CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA
    ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT
    CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT
    TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC
    TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC
    GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC
    TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC
    ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC
    GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT
    GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG
    GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG
    CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC
    CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC
    TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG
    GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC
    TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG
    AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG
    GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA
    TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG
    AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG
    TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC
    CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC
    AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT
    CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT
    CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT
    GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT
    CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC
    ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC
    CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC
    AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT
    CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT
    GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC
    GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT
    CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA
    GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92273
    Clone ID Related Accession (Same CDS sequence) XM_015474062.1
    Accession Version XM_015474062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA
    GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC
    AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA
    AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG
    AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG
    GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC
    AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC
    TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT
    GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC
    GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA
    GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC
    CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA
    GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT
    GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT
    GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG
    CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG
    GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC
    TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT
    GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC
    GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT
    GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA
    CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC
    GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC
    GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT
    GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG
    CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG
    TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC
    TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC
    GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC
    ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG
    TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC
    CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGC AGAGAGGAGG
    AGAAGGAGCC TGGGGCTGAT CCCGCTGACA GCGCGCATGG TGATGGCGCA CCTGGTCAAC
    CACCTGGGCC ACTTCCCGCT CTGCGGGGGC CCCGCCGTGC TGCACAGCCT GGTGGGCGAG
    CACCACGACA ACGTGCACGC CGACGCCACC GAGCTCTCCC CAGACGTGTT CAGGAGCCCC
    AGCCTGCAGC TGTTTGTGTT CAACGACAGC ACCCTCATCT CCTACCTGCA GACGCCTGCG
    ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC GCCCCCTCGG ACGTGAGAGT GATCGTGAGG
    GACATCTCAG GGAAGTACTC GTGGGACGGG AAGGTGCTCT ACGGACCCCT GGAAGGCCAC
    TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC CTCATCTCGA GCTGTCAACC CCCAACCCCT
    GGACCTCAGA GGGCTCCTTC TCAGACTGAG GAGGGAGAGG ATGTCCTCGA CAAATTACTT
    GAAAACCTTG GCCACACGAG TCCTGAATGC CTTTTGCCAT CCCAGCTCCG TCTGAATGAG
    CCTTCCCCAC CCCCTTGTGG CATGAATTGG GACCAGGAGA AGGAGATCAC CAAGGTCCTG
    CTGCGCCAGA GCACGCAAGA AGACGAGTAT GTCCAGAGGT GCAACTCGGA TGTGGCCATG
    AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA GTGGAGCCCC GGGGCGCCTT CTACTTCTGT
    AGGCTGCTGC TCGATGACTT GGGAATGAAT TCTTGGGACA GAAGGAAGAA CTTCCATCTG
    TTGAAGAAAA ATTCAAAATT ATTGAGAGAG CTAAAAAATC TGGACTCCCG GCAGTGCCGT
    GAAACGCACA AAATCGCAGT GTTTTACATT GCCGAAGGTC AAGAAGACAA GTGTTCGATC
    CTTTCCAATG AAAGGGGAAG CCAAGCGTAT GAAGACTTTG TTGCTGGACT TGGATGGGAG
    GTGGACCTGT CAACCCACTG TGGGTTCATG GGCGGGCTTC AGCGCAATGG CAGCACGGGC
    CAGACCGCCC CCTACTACGC TACCTCGACC GTGGAAGTCA TCTTCCACGT CTCAACTCGG
    ATGCCGTCAG ACTCAGACGA CTCGCTCACC AAAAAGCTCC GTCACTTGGG GAATGACGAA
    GTCCACATCG TCTGGTCCGA GCACTCCAGG GACTACCGCA GCGGGATCAT CCCCACCGCC
    TTCGGAGACG TTTCAATCAT CATCTACCCC ATGAAGAATC ACATGTTCTT TATCGCGATA
    ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG
    AAGCTACTGC CAAGCCTTAT CTGTGCCACC TGCATCAACG CCAGCCGGGC CGTCAAGTGC
    CTCATCCCCC TCTACCAGAG CTTCTACGAG GAGAGAGCTC TCTATCTGGA AGCAATAATT
    CAGAACCACC GTGAAGTCAT GACATTTGAG GATTTCACAG CCCAGGTCTT CTGTCCATCT
    CCCAGCTACT CGGTCAGCGG AACTGTGTCA GCTCTGGAAG AGGGAAGAGA TGGATGTGAT
    GAACATCTGC TCCACCTGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329548.1
    CDS16..5619
    Translation

    Target ORF information:

    RefSeq Version XM_015474062.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA
    GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC
    AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA
    AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG
    AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG
    GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC
    AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC
    TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT
    GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC
    GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA
    GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC
    CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA
    GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT
    GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT
    GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG
    CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG
    GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC
    TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT
    GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC
    GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT
    GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA
    CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC
    GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC
    GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT
    GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG
    CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG
    TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC
    TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC
    GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC
    ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG
    TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC
    CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGC AGAGAGGAGG
    AGAAGGAGCC TGGGGCTGAT CCCGCTGACA GCGCGCATGG TGATGGCGCA CCTGGTCAAC
    CACCTGGGCC ACTTCCCGCT CTGCGGGGGC CCCGCCGTGC TGCACAGCCT GGTGGGCGAG
    CACCACGACA ACGTGCACGC CGACGCCACC GAGCTCTCCC CAGACGTGTT CAGGAGCCCC
    AGCCTGCAGC TGTTTGTGTT CAACGACAGC ACCCTCATCT CCTACCTGCA GACGCCTGCG
    ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC GCCCCCTCGG ACGTGAGAGT GATCGTGAGG
    GACATCTCAG GGAAGTACTC GTGGGACGGG AAGGTGCTCT ACGGACCCCT GGAAGGCCAC
    TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC CTCATCTCGA GCTGTCAACC CCCAACCCCT
    GGACCTCAGA GGGCTCCTTC TCAGACTGAG GAGGGAGAGG ATGTCCTCGA CAAATTACTT
    GAAAACCTTG GCCACACGAG TCCTGAATGC CTTTTGCCAT CCCAGCTCCG TCTGAATGAG
    CCTTCCCCAC CCCCTTGTGG CATGAATTGG GACCAGGAGA AGGAGATCAC CAAGGTCCTG
    CTGCGCCAGA GCACGCAAGA AGACGAGTAT GTCCAGAGGT GCAACTCGGA TGTGGCCATG
    AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA GTGGAGCCCC GGGGCGCCTT CTACTTCTGT
    AGGCTGCTGC TCGATGACTT GGGAATGAAT TCTTGGGACA GAAGGAAGAA CTTCCATCTG
    TTGAAGAAAA ATTCAAAATT ATTGAGAGAG CTAAAAAATC TGGACTCCCG GCAGTGCCGT
    GAAACGCACA AAATCGCAGT GTTTTACATT GCCGAAGGTC AAGAAGACAA GTGTTCGATC
    CTTTCCAATG AAAGGGGAAG CCAAGCGTAT GAAGACTTTG TTGCTGGACT TGGATGGGAG
    GTGGACCTGT CAACCCACTG TGGGTTCATG GGCGGGCTTC AGCGCAATGG CAGCACGGGC
    CAGACCGCCC CCTACTACGC TACCTCGACC GTGGAAGTCA TCTTCCACGT CTCAACTCGG
    ATGCCGTCAG ACTCAGACGA CTCGCTCACC AAAAAGCTCC GTCACTTGGG GAATGACGAA
    GTCCACATCG TCTGGTCCGA GCACTCCAGG GACTACCGCA GCGGGATCAT CCCCACCGCC
    TTCGGAGACG TTTCAATCAT CATCTACCCC ATGAAGAATC ACATGTTCTT TATCGCGATA
    ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG
    AAGCTACTGC CAAGCCTTAT CTGTGCCACC TGCATCAACG CCAGCCGGGC CGTCAAGTGC
    CTCATCCCCC TCTACCAGAG CTTCTACGAG GAGAGAGCTC TCTATCTGGA AGCAATAATT
    CAGAACCACC GTGAAGTCAT GACATTTGAG GATTTCACAG CCCAGGTCTT CTGTCCATCT
    CCCAGCTACT CGGTCAGCGG AACTGTGTCA GCTCTGGAAG AGGGAAGAGA TGGATGTGAT
    GAACATCTGC TCCACCTGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92274
    Clone ID Related Accession (Same CDS sequence) XM_015474063.1
    Accession Version XM_015474063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA
    GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC
    AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA
    AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG
    AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG
    GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC
    AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC
    TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT
    GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC
    GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA
    GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC
    CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA
    GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT
    GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT
    GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG
    CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG
    GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC
    TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT
    GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC
    GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT
    GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA
    CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC
    GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC
    GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT
    GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG
    CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG
    TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC
    TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC
    GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC
    ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG
    TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC
    CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGA GAGGAGGAGA
    AGGAGCCTGG GGCTGATCCC GCTGACAGCG CGCATGGTGA TGGCGCACCT GGTCAACCAC
    CTGGGCCACT TCCCGCTCTG CGGGGGCCCC GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC
    CACGACAACG TGCACGCCGA CGCCACCGAG CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC
    CTGCAGCTGT TTGTGTTCAA CGACAGCACC CTCATCTCCT ACCTGCAGAC GCCTGCGACC
    GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC CCCTCGGACG TGAGAGTGAT CGTGAGGGAC
    ATCTCAGGGA AGTACTCGTG GGACGGGAAG GTGCTCTACG GACCCCTGGA AGGCCACTTA
    GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC ATCTCGAGCT GTCAACCCCC AACCCCTGGA
    CCTCAGAGGG CTCCTTCTCA GACTGAGGAG GGAGAGGATG TCCTCGACAA ATTACTTGAA
    AACCTTGGCC ACACGAGTCC TGAATGCCTT TTGCCATCCC AGCTCCGTCT GAATGAGCCT
    TCCCCACCCC CTTGTGGCAT GAATTGGGAC CAGGAGAAGG AGATCACCAA GGTCCTGCTG
    CGCCAGAGCA CGCAAGAAGA CGAGTATGTC CAGAGGTGCA ACTCGGATGT GGCCATGAGG
    GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG
    CTGCTGCTCG ATGACTTGGG AATGAATTCT TGGGACAGAA GGAAGAACTT CCATCTGTTG
    AAGAAAAATT CAAAATTATT GAGAGAGCTA AAAAATCTGG ACTCCCGGCA GTGCCGTGAA
    ACGCACAAAA TCGCAGTGTT TTACATTGCC GAAGGTCAAG AAGACAAGTG TTCGATCCTT
    TCCAATGAAA GGGGAAGCCA AGCGTATGAA GACTTTGTTG CTGGACTTGG ATGGGAGGTG
    GACCTGTCAA CCCACTGTGG GTTCATGGGC GGGCTTCAGC GCAATGGCAG CACGGGCCAG
    ACCGCCCCCT ACTACGCTAC CTCGACCGTG GAAGTCATCT TCCACGTCTC AACTCGGATG
    CCGTCAGACT CAGACGACTC GCTCACCAAA AAGCTCCGTC ACTTGGGGAA TGACGAAGTC
    CACATCGTCT GGTCCGAGCA CTCCAGGGAC TACCGCAGCG GGATCATCCC CACCGCCTTC
    GGAGACGTTT CAATCATCAT CTACCCCATG AAGAATCACA TGTTCTTTAT CGCGATAACC
    AAGAAACCCG AGGTTCCATT TTTTGGGCCT CTGTTTGATG GAGCAATCGT GAGTGGGAAG
    CTACTGCCAA GCCTTATCTG TGCCACCTGC ATCAACGCCA GCCGGGCCGT CAAGTGCCTC
    ATCCCCCTCT ACCAGAGCTT CTACGAGGAG AGAGCTCTCT ATCTGGAAGC AATAATTCAG
    AACCACCGTG AAGTCATGAC ATTTGAGGAT TTCACAGCCC AGGTCTTCTG TCCATCTCCC
    AGCTACTCGG TCAGCGGAAC TGTGTCAGCT CTGGAAGAGG GAAGAGATGG ATGTGATGAA
    CATCTGCTCC ACCTGATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329549.1
    CDS16..5616
    Translation

    Target ORF information:

    RefSeq Version XM_015474063.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT
    TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC
    GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC
    GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA
    GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC
    AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA
    AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG
    AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG
    GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC
    AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC
    TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT
    GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC
    GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA
    GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC
    CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA
    GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT
    GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT
    GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG
    CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG
    GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC
    TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT
    GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC
    GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT
    GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA
    CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC
    GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC
    GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT
    GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG
    CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG
    TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC
    TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC
    GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC
    ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG
    TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC
    CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGA GAGGAGGAGA
    AGGAGCCTGG GGCTGATCCC GCTGACAGCG CGCATGGTGA TGGCGCACCT GGTCAACCAC
    CTGGGCCACT TCCCGCTCTG CGGGGGCCCC GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC
    CACGACAACG TGCACGCCGA CGCCACCGAG CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC
    CTGCAGCTGT TTGTGTTCAA CGACAGCACC CTCATCTCCT ACCTGCAGAC GCCTGCGACC
    GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC CCCTCGGACG TGAGAGTGAT CGTGAGGGAC
    ATCTCAGGGA AGTACTCGTG GGACGGGAAG GTGCTCTACG GACCCCTGGA AGGCCACTTA
    GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC ATCTCGAGCT GTCAACCCCC AACCCCTGGA
    CCTCAGAGGG CTCCTTCTCA GACTGAGGAG GGAGAGGATG TCCTCGACAA ATTACTTGAA
    AACCTTGGCC ACACGAGTCC TGAATGCCTT TTGCCATCCC AGCTCCGTCT GAATGAGCCT
    TCCCCACCCC CTTGTGGCAT GAATTGGGAC CAGGAGAAGG AGATCACCAA GGTCCTGCTG
    CGCCAGAGCA CGCAAGAAGA CGAGTATGTC CAGAGGTGCA ACTCGGATGT GGCCATGAGG
    GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG
    CTGCTGCTCG ATGACTTGGG AATGAATTCT TGGGACAGAA GGAAGAACTT CCATCTGTTG
    AAGAAAAATT CAAAATTATT GAGAGAGCTA AAAAATCTGG ACTCCCGGCA GTGCCGTGAA
    ACGCACAAAA TCGCAGTGTT TTACATTGCC GAAGGTCAAG AAGACAAGTG TTCGATCCTT
    TCCAATGAAA GGGGAAGCCA AGCGTATGAA GACTTTGTTG CTGGACTTGG ATGGGAGGTG
    GACCTGTCAA CCCACTGTGG GTTCATGGGC GGGCTTCAGC GCAATGGCAG CACGGGCCAG
    ACCGCCCCCT ACTACGCTAC CTCGACCGTG GAAGTCATCT TCCACGTCTC AACTCGGATG
    CCGTCAGACT CAGACGACTC GCTCACCAAA AAGCTCCGTC ACTTGGGGAA TGACGAAGTC
    CACATCGTCT GGTCCGAGCA CTCCAGGGAC TACCGCAGCG GGATCATCCC CACCGCCTTC
    GGAGACGTTT CAATCATCAT CTACCCCATG AAGAATCACA TGTTCTTTAT CGCGATAACC
    AAGAAACCCG AGGTTCCATT TTTTGGGCCT CTGTTTGATG GAGCAATCGT GAGTGGGAAG
    CTACTGCCAA GCCTTATCTG TGCCACCTGC ATCAACGCCA GCCGGGCCGT CAAGTGCCTC
    ATCCCCCTCT ACCAGAGCTT CTACGAGGAG AGAGCTCTCT ATCTGGAAGC AATAATTCAG
    AACCACCGTG AAGTCATGAC ATTTGAGGAT TTCACAGCCC AGGTCTTCTG TCCATCTCCC
    AGCTACTCGG TCAGCGGAAC TGTGTCAGCT CTGGAAGAGG GAAGAGATGG ATGTGATGAA
    CATCTGCTCC ACCTGATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92275
    Clone ID Related Accession (Same CDS sequence) XM_015474064.1
    Accession Version XM_015474064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGAAGT GGAAGAATAC
    CTGCAGCCAG AAAACACACC TGCCACCGAA TCTGGGCCCC TGGGGCAGCA GCCGATGGTT
    CGGAGCGGCA GCAGCCCTGA GGTCACCGAG CCGCTGTGTC CTGAGCCCCC CCCAGGCCAA
    AAGCTAGAAA ATGCACAGAA CGTGAGTTCT TCAGAGCCCA GACTTGCTCA GGAGAGCAAA
    GGACATGAGA AGAAAGAGCA TGAAGGAATA ACAATCTTAG TTCGAAGAAG CAGCAGCCCC
    ATTGAACTGG ATTTGAAAGA TGACTCACAG CAGATGCAAG GAAAACGTAG GGAGAGACAG
    AAGAGTGACA GTGTGAGCAG TGACCCCACT CTGGGCTGCA GCACTGAGGC AGAGCTGTCC
    CTGAGCCACT GGCAGACCTG CGAGGAAGAC CCAGATCTGA GCCCGCCTGC AGATACTGTG
    ACCGACGCTG ACGCCCGCCA CTGGTTACAG CTCCGGCCCA CGGATGCTTC AAACCTAACA
    GACAGCAGCG AATGCCTTGC TGATGACTGC AGTATAATTG CTGGGGGGAC CCTCACTGGT
    TGGCACCCAG ACTCAGCCGC TGTGTTGTGG CGAAGAATCT TGGGAATCCT TGGAGATGTG
    AATAATATCC AGTCACCCAA AATCCATGCC AGGGTGTTTG GCTATCTCTA TGAACTCTGG
    TACAAGCTAG CAAAGATACG GGATAACCTG GCAATAAGCC TGGATAACCA GTCTTCTCCA
    TCTCCTCCTG CCTTGATCCC ACCGCTCAGA ATGTTTGCAT CATGGCTATT TAAGGCGACG
    ACGATGCCTG ACGATTATAA GGAAGGCAAA CTGCAAGCCT ATAAGCTGAT CTGCTCCATG
    ATGACCCGGC GCCAGGACGT CCTGCCCAAC TCAGACTTCC TGGTGCATTT TTACCTCGTC
    ATGCACCTGG GACTGACCAG CGAGGACCAG GATATCCTGA ATACCATCAT AAGGCACTGC
    CCGCCCCGCT TCTTCTCCCT GGGCTTGCCT GGCTTCTCCA TGCTGGTGGG GGACTTCATC
    GTGGCTGCTG GCAGGGTCCT CAGTGCAGAC ACGCTGGCGG CACCTCGTTC GGAAGCCCTC
    ACCATTCTCG GGTCTCTGGT CTGCTTTCCG AACACCTACC GCGAGATTCC CCTACGGCAG
    CCGGTACCTG AAGTTGCTGA AGTCGCCATG GGAGCAGAAG ATGTCAAGCA CTGCCTCATA
    AGTATCTTAC TGAAGAATGC CACAGAGGAA CCGAGTGAGT GTGCAAGGTG CATTGCCATC
    TGCTCCCTCG GGATCTGGAT CTGTGAGGAG CTGGCACAAG GCACTGGCCA CCCTCAGCTG
    CAAGAGGCCG TCAACGTCAT TGGTGTCACG TTGAAGTTTC CTAACAAAAT TGTGGCCCAG
    GTAGCCTGTG ACGTGCTTCA GCTGCTAGTC TCCTACTGGG AAAAGCTTCA GATGTTTGAA
    ACCTCCTTGC CTCGGAAAAT TGCAGAAATC CTTGTGGCCA CAATTGCTTT TCTGTTACCA
    AATGCAGAGT ACTCCTCGGT GGAGACAGAC AAGAAGTTCC TCGTGTCCTT GTTGCTCTGC
    CTCCTGGACT GGTGCATGGC GCTGCCGGCA GGAACCCTCC TCCACCCCGT GTCCACGGCG
    GCCCTGGACG AACAGGCCCC GGCCCGGGCC CCCTTACTGG ATTACATCTA CAGGGTTCTG
    CACTGCTGCA TCTGTGGCTC AAGCACGTAC AGCCAGCAGA GCCGCTACAC CTTGACCCTG
    GCGGACCTGT CGTCCTCGGA TTACGACCCC TTCCTGCCGC TGGCGAACGT GAAGAATTCT
    GAGCCGGTCC AATTTCATTC ATCAGCAGAA CTGGGCAACC TGCTCACTGT TGAAGAGGCA
    GAGAGGAGGA GAAGGAGCCT GGGGCTGATC CCGCTGACAG CGCGCATGGT GATGGCGCAC
    CTGGTCAACC ACCTGGGCCA CTTCCCGCTC TGCGGGGGCC CCGCCGTGCT GCACAGCCTG
    GTGGGCGAGC ACCACGACAA CGTGCACGCC GACGCCACCG AGCTCTCCCC AGACGTGTTC
    AGGAGCCCCA GCCTGCAGCT GTTTGTGTTC AACGACAGCA CCCTCATCTC CTACCTGCAG
    ACGCCTGCGA CCGGTCCTGC CAGTGGGTCG CCAGCGGGCG CCCCCTCGGA CGTGAGAGTG
    ATCGTGAGGG ACATCTCAGG GAAGTACTCG TGGGACGGGA AGGTGCTCTA CGGACCCCTG
    GAAGGCCACT TAGCCCCCAA GGGCAGAAGC CCCTCGTTCC TCATCTCGAG CTGTCAACCC
    CCAACCCCTG GACCTCAGAG GGCTCCTTCT CAGACTGAGG AGGGAGAGGA TGTCCTCGAC
    AAATTACTTG AAAACCTTGG CCACACGAGT CCTGAATGCC TTTTGCCATC CCAGCTCCGT
    CTGAATGAGC CTTCCCCACC CCCTTGTGGC ATGAATTGGG ACCAGGAGAA GGAGATCACC
    AAGGTCCTGC TGCGCCAGAG CACGCAAGAA GACGAGTATG TCCAGAGGTG CAACTCGGAT
    GTGGCCATGA GGGTCGCCAG CCAGGAGCCG CCGTCCCCAG TGGAGCCCCG GGGCGCCTTC
    TACTTCTGTA GGCTGCTGCT CGATGACTTG GGAATGAATT CTTGGGACAG AAGGAAGAAC
    TTCCATCTGT TGAAGAAAAA TTCAAAATTA TTGAGAGAGC TAAAAAATCT GGACTCCCGG
    CAGTGCCGTG AAACGCACAA AATCGCAGTG TTTTACATTG CCGAAGGTCA AGAAGACAAG
    TGTTCGATCC TTTCCAATGA AAGGGGAAGC CAAGCGTATG AAGACTTTGT TGCTGGACTT
    GGATGGGAGG TGGACCTGTC AACCCACTGT GGGTTCATGG GCGGGCTTCA GCGCAATGGC
    AGCACGGGCC AGACCGCCCC CTACTACGCT ACCTCGACCG TGGAAGTCAT CTTCCACGTC
    TCAACTCGGA TGCCGTCAGA CTCAGACGAC TCGCTCACCA AAAAGCTCCG TCACTTGGGG
    AATGACGAAG TCCACATCGT CTGGTCCGAG CACTCCAGGG ACTACCGCAG CGGGATCATC
    CCCACCGCCT TCGGAGACGT TTCAATCATC ATCTACCCCA TGAAGAATCA CATGTTCTTT
    ATCGCGATAA CCAAGAAACC CGAGGTTCCA TTTTTTGGGC CTCTGTTTGA TGGAGCAATC
    GTGAGTGGGA AGCTACTGCC AAGCCTTATC TGTGCCACCT GCATCAACGC CAGCCGGGCC
    GTCAAGTGCC TCATCCCCCT CTACCAGAGC TTCTACGAGG AGAGAGCTCT CTATCTGGAA
    GCAATAATTC AGAACCACCG TGAAGTCATG ACATTTGAGG ATTTCACAGC CCAGGTCTTC
    TGTCCATCTC CCAGCTACTC GGTCAGCGGA ACTGTGTCAG CTCTGGAAGA GGGAAGAGAT
    GGATGTGATG AACATCTGCT CCACCTGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329550.1
    CDS16..5448
    Translation

    Target ORF information:

    RefSeq Version XM_015474064.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
    TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG
    GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT
    CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT
    TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA
    AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA
    GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC
    AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG
    ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG
    GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC
    TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT
    CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG
    CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC
    AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG
    TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT
    GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG
    CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG
    AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC
    CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC
    GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA
    AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA
    CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT
    AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT
    TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG
    AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG
    CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT
    GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA
    CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA
    CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG
    GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC
    GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG
    GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT
    CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGAAGT GGAAGAATAC
    CTGCAGCCAG AAAACACACC TGCCACCGAA TCTGGGCCCC TGGGGCAGCA GCCGATGGTT
    CGGAGCGGCA GCAGCCCTGA GGTCACCGAG CCGCTGTGTC CTGAGCCCCC CCCAGGCCAA
    AAGCTAGAAA ATGCACAGAA CGTGAGTTCT TCAGAGCCCA GACTTGCTCA GGAGAGCAAA
    GGACATGAGA AGAAAGAGCA TGAAGGAATA ACAATCTTAG TTCGAAGAAG CAGCAGCCCC
    ATTGAACTGG ATTTGAAAGA TGACTCACAG CAGATGCAAG GAAAACGTAG GGAGAGACAG
    AAGAGTGACA GTGTGAGCAG TGACCCCACT CTGGGCTGCA GCACTGAGGC AGAGCTGTCC
    CTGAGCCACT GGCAGACCTG CGAGGAAGAC CCAGATCTGA GCCCGCCTGC AGATACTGTG
    ACCGACGCTG ACGCCCGCCA CTGGTTACAG CTCCGGCCCA CGGATGCTTC AAACCTAACA
    GACAGCAGCG AATGCCTTGC TGATGACTGC AGTATAATTG CTGGGGGGAC CCTCACTGGT
    TGGCACCCAG ACTCAGCCGC TGTGTTGTGG CGAAGAATCT TGGGAATCCT TGGAGATGTG
    AATAATATCC AGTCACCCAA AATCCATGCC AGGGTGTTTG GCTATCTCTA TGAACTCTGG
    TACAAGCTAG CAAAGATACG GGATAACCTG GCAATAAGCC TGGATAACCA GTCTTCTCCA
    TCTCCTCCTG CCTTGATCCC ACCGCTCAGA ATGTTTGCAT CATGGCTATT TAAGGCGACG
    ACGATGCCTG ACGATTATAA GGAAGGCAAA CTGCAAGCCT ATAAGCTGAT CTGCTCCATG
    ATGACCCGGC GCCAGGACGT CCTGCCCAAC TCAGACTTCC TGGTGCATTT TTACCTCGTC
    ATGCACCTGG GACTGACCAG CGAGGACCAG GATATCCTGA ATACCATCAT AAGGCACTGC
    CCGCCCCGCT TCTTCTCCCT GGGCTTGCCT GGCTTCTCCA TGCTGGTGGG GGACTTCATC
    GTGGCTGCTG GCAGGGTCCT CAGTGCAGAC ACGCTGGCGG CACCTCGTTC GGAAGCCCTC
    ACCATTCTCG GGTCTCTGGT CTGCTTTCCG AACACCTACC GCGAGATTCC CCTACGGCAG
    CCGGTACCTG AAGTTGCTGA AGTCGCCATG GGAGCAGAAG ATGTCAAGCA CTGCCTCATA
    AGTATCTTAC TGAAGAATGC CACAGAGGAA CCGAGTGAGT GTGCAAGGTG CATTGCCATC
    TGCTCCCTCG GGATCTGGAT CTGTGAGGAG CTGGCACAAG GCACTGGCCA CCCTCAGCTG
    CAAGAGGCCG TCAACGTCAT TGGTGTCACG TTGAAGTTTC CTAACAAAAT TGTGGCCCAG
    GTAGCCTGTG ACGTGCTTCA GCTGCTAGTC TCCTACTGGG AAAAGCTTCA GATGTTTGAA
    ACCTCCTTGC CTCGGAAAAT TGCAGAAATC CTTGTGGCCA CAATTGCTTT TCTGTTACCA
    AATGCAGAGT ACTCCTCGGT GGAGACAGAC AAGAAGTTCC TCGTGTCCTT GTTGCTCTGC
    CTCCTGGACT GGTGCATGGC GCTGCCGGCA GGAACCCTCC TCCACCCCGT GTCCACGGCG
    GCCCTGGACG AACAGGCCCC GGCCCGGGCC CCCTTACTGG ATTACATCTA CAGGGTTCTG
    CACTGCTGCA TCTGTGGCTC AAGCACGTAC AGCCAGCAGA GCCGCTACAC CTTGACCCTG
    GCGGACCTGT CGTCCTCGGA TTACGACCCC TTCCTGCCGC TGGCGAACGT GAAGAATTCT
    GAGCCGGTCC AATTTCATTC ATCAGCAGAA CTGGGCAACC TGCTCACTGT TGAAGAGGCA
    GAGAGGAGGA GAAGGAGCCT GGGGCTGATC CCGCTGACAG CGCGCATGGT GATGGCGCAC
    CTGGTCAACC ACCTGGGCCA CTTCCCGCTC TGCGGGGGCC CCGCCGTGCT GCACAGCCTG
    GTGGGCGAGC ACCACGACAA CGTGCACGCC GACGCCACCG AGCTCTCCCC AGACGTGTTC
    AGGAGCCCCA GCCTGCAGCT GTTTGTGTTC AACGACAGCA CCCTCATCTC CTACCTGCAG
    ACGCCTGCGA CCGGTCCTGC CAGTGGGTCG CCAGCGGGCG CCCCCTCGGA CGTGAGAGTG
    ATCGTGAGGG ACATCTCAGG GAAGTACTCG TGGGACGGGA AGGTGCTCTA CGGACCCCTG
    GAAGGCCACT TAGCCCCCAA GGGCAGAAGC CCCTCGTTCC TCATCTCGAG CTGTCAACCC
    CCAACCCCTG GACCTCAGAG GGCTCCTTCT CAGACTGAGG AGGGAGAGGA TGTCCTCGAC
    AAATTACTTG AAAACCTTGG CCACACGAGT CCTGAATGCC TTTTGCCATC CCAGCTCCGT
    CTGAATGAGC CTTCCCCACC CCCTTGTGGC ATGAATTGGG ACCAGGAGAA GGAGATCACC
    AAGGTCCTGC TGCGCCAGAG CACGCAAGAA GACGAGTATG TCCAGAGGTG CAACTCGGAT
    GTGGCCATGA GGGTCGCCAG CCAGGAGCCG CCGTCCCCAG TGGAGCCCCG GGGCGCCTTC
    TACTTCTGTA GGCTGCTGCT CGATGACTTG GGAATGAATT CTTGGGACAG AAGGAAGAAC
    TTCCATCTGT TGAAGAAAAA TTCAAAATTA TTGAGAGAGC TAAAAAATCT GGACTCCCGG
    CAGTGCCGTG AAACGCACAA AATCGCAGTG TTTTACATTG CCGAAGGTCA AGAAGACAAG
    TGTTCGATCC TTTCCAATGA AAGGGGAAGC CAAGCGTATG AAGACTTTGT TGCTGGACTT
    GGATGGGAGG TGGACCTGTC AACCCACTGT GGGTTCATGG GCGGGCTTCA GCGCAATGGC
    AGCACGGGCC AGACCGCCCC CTACTACGCT ACCTCGACCG TGGAAGTCAT CTTCCACGTC
    TCAACTCGGA TGCCGTCAGA CTCAGACGAC TCGCTCACCA AAAAGCTCCG TCACTTGGGG
    AATGACGAAG TCCACATCGT CTGGTCCGAG CACTCCAGGG ACTACCGCAG CGGGATCATC
    CCCACCGCCT TCGGAGACGT TTCAATCATC ATCTACCCCA TGAAGAATCA CATGTTCTTT
    ATCGCGATAA CCAAGAAACC CGAGGTTCCA TTTTTTGGGC CTCTGTTTGA TGGAGCAATC
    GTGAGTGGGA AGCTACTGCC AAGCCTTATC TGTGCCACCT GCATCAACGC CAGCCGGGCC
    GTCAAGTGCC TCATCCCCCT CTACCAGAGC TTCTACGAGG AGAGAGCTCT CTATCTGGAA
    GCAATAATTC AGAACCACCG TGAAGTCATG ACATTTGAGG ATTTCACAGC CCAGGTCTTC
    TGTCCATCTC CCAGCTACTC GGTCAGCGGA ACTGTGTCAG CTCTGGAAGA GGGAAGAGAT
    GGATGTGATG AACATCTGCT CCACCTGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94787
    Clone ID Related Accession (Same CDS sequence) XM_015465968.1
    Accession Version XM_015465968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGTGTCA
    GCTCTGGAAG AGGGAAGAGA TGGATGTGAT GAACATCTGC TCCACCTGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321454.1
    CDS375..5708
    Translation

    Target ORF information:

    RefSeq Version XM_015465968.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGTGTCA
    GCTCTGGAAG AGGGAAGAGA TGGATGTGAT GAACATCTGC TCCACCTGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94788
    Clone ID Related Accession (Same CDS sequence) XM_015465969.1
    Accession Version XM_015465969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGA GAGGAGGAGA AGGAGCCTGG GGCTGATCCC GCTGACAGCG
    CGCATGGTGA TGGCGCACCT GGTCAACCAC CTGGGCCACT TCCCGCTCTG CGGGGGCCCC
    GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC CACGACAACG TGCACGCCGA CGCCACCGAG
    CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC CTGCAGCTGT TTGTGTTCAA CGACAGCACC
    CTCATCTCCT ACCTGCAGAC GCCTGCGACC GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC
    CCCTCGGACG TGAGAGTGAT CGTGAGGGAC ATCTCAGGGA AGTACTCGTG GGACGGGAAG
    GTGCTCTACG GACCCCTGGA AGGCCACTTA GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC
    ATCTCGAGCT GTCAACCCCC AACCCCTGGA CCTCAGAGGG CTCCTTCTCA GACTGAGGAG
    GGAGAGGATG TCCTCGACAA ATTACTTGAA AACCTTGGCC ACACGAGTCC TGAATGCCTT
    TTGCCATCCC AGCTCCGTCT GAATGAGCCT TCCCCACCCC CTTGTGGCAT GAATTGGGAC
    CAGGAGAAGG AGATCACCAA GGTCCTGCTG CGCCAGAGCA CGCAAGAAGA CGAGTATGTC
    CAGAGGTGCA ACTCGGATGT GGCCATGAGG GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG
    GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG CTGCTGCTCG ATGACTTGGG AATGAATTCT
    TGGGACAGAA GGAAGAACTT CCATCTGTTG AAGAAAAATT CAAAATTATT GAGAGAGCTA
    AAAAATCTGG ACTCCCGGCA GTGCCGTGAA ACGCACAAAA TCGCAGTGTT TTACATTGCC
    GAAGGTCAAG AAGACAAGTG TTCGATCCTT TCCAATGAAA GGGGAAGCCA AGCGTATGAA
    GACTTTGTTG CTGGACTTGG ATGGGAGGTG GACCTGTCAA CCCACTGTGG GTTCATGGGC
    GGGCTTCAGC GCAATGGCAG CACGGGCCAG ACCGCCCCCT ACTACGCTAC CTCGACCGTG
    GAAGTCATCT TCCACGTCTC AACTCGGATG CCGTCAGACT CAGACGACTC GCTCACCAAA
    AAGCTCCGTC ACTTGGGGAA TGACGAAGTC CACATCGTCT GGTCCGAGCA CTCCAGGGAC
    TACCGCAGCG GGATCATCCC CACCGCCTTC GGAGACGTTT CAATCATCAT CTACCCCATG
    AAGAATCACA TGTTCTTTAT CGCGATAACC AAGAAACCCG AGGTTCCATT TTTTGGGCCT
    CTGTTTGATG GAGCAATCGT GAGTGGGAAG CTACTGCCAA GCCTTATCTG TGCCACCTGC
    ATCAACGCCA GCCGGGCCGT CAAGTGCCTC ATCCCCCTCT ACCAGAGCTT CTACGAGGAG
    AGAGCTCTCT ATCTGGAAGC AATAATTCAG AACCACCGTG AAGTCATGAC ATTTGAGGAT
    TTCACAGCCC AGGTCTTCTG TCCATCTCCC AGCTACTCGG TCAGCGGAAC TGTGTCAGCT
    CTGGAAGAGG GAAGAGATGG ATGTGATGAA CATCTGCTCC ACCTGATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321455.1
    CDS375..5705
    Translation

    Target ORF information:

    RefSeq Version XM_015465969.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGA GAGGAGGAGA AGGAGCCTGG GGCTGATCCC GCTGACAGCG
    CGCATGGTGA TGGCGCACCT GGTCAACCAC CTGGGCCACT TCCCGCTCTG CGGGGGCCCC
    GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC CACGACAACG TGCACGCCGA CGCCACCGAG
    CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC CTGCAGCTGT TTGTGTTCAA CGACAGCACC
    CTCATCTCCT ACCTGCAGAC GCCTGCGACC GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC
    CCCTCGGACG TGAGAGTGAT CGTGAGGGAC ATCTCAGGGA AGTACTCGTG GGACGGGAAG
    GTGCTCTACG GACCCCTGGA AGGCCACTTA GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC
    ATCTCGAGCT GTCAACCCCC AACCCCTGGA CCTCAGAGGG CTCCTTCTCA GACTGAGGAG
    GGAGAGGATG TCCTCGACAA ATTACTTGAA AACCTTGGCC ACACGAGTCC TGAATGCCTT
    TTGCCATCCC AGCTCCGTCT GAATGAGCCT TCCCCACCCC CTTGTGGCAT GAATTGGGAC
    CAGGAGAAGG AGATCACCAA GGTCCTGCTG CGCCAGAGCA CGCAAGAAGA CGAGTATGTC
    CAGAGGTGCA ACTCGGATGT GGCCATGAGG GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG
    GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG CTGCTGCTCG ATGACTTGGG AATGAATTCT
    TGGGACAGAA GGAAGAACTT CCATCTGTTG AAGAAAAATT CAAAATTATT GAGAGAGCTA
    AAAAATCTGG ACTCCCGGCA GTGCCGTGAA ACGCACAAAA TCGCAGTGTT TTACATTGCC
    GAAGGTCAAG AAGACAAGTG TTCGATCCTT TCCAATGAAA GGGGAAGCCA AGCGTATGAA
    GACTTTGTTG CTGGACTTGG ATGGGAGGTG GACCTGTCAA CCCACTGTGG GTTCATGGGC
    GGGCTTCAGC GCAATGGCAG CACGGGCCAG ACCGCCCCCT ACTACGCTAC CTCGACCGTG
    GAAGTCATCT TCCACGTCTC AACTCGGATG CCGTCAGACT CAGACGACTC GCTCACCAAA
    AAGCTCCGTC ACTTGGGGAA TGACGAAGTC CACATCGTCT GGTCCGAGCA CTCCAGGGAC
    TACCGCAGCG GGATCATCCC CACCGCCTTC GGAGACGTTT CAATCATCAT CTACCCCATG
    AAGAATCACA TGTTCTTTAT CGCGATAACC AAGAAACCCG AGGTTCCATT TTTTGGGCCT
    CTGTTTGATG GAGCAATCGT GAGTGGGAAG CTACTGCCAA GCCTTATCTG TGCCACCTGC
    ATCAACGCCA GCCGGGCCGT CAAGTGCCTC ATCCCCCTCT ACCAGAGCTT CTACGAGGAG
    AGAGCTCTCT ATCTGGAAGC AATAATTCAG AACCACCGTG AAGTCATGAC ATTTGAGGAT
    TTCACAGCCC AGGTCTTCTG TCCATCTCCC AGCTACTCGG TCAGCGGAAC TGTGTCAGCT
    CTGGAAGAGG GAAGAGATGG ATGTGATGAA CATCTGCTCC ACCTGATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94789
    Clone ID Related Accession (Same CDS sequence) XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
    Accession Version XM_015465971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA
    GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321457.1
    CDS375..5693
    Translation

    Target ORF information:

    RefSeq Version XM_015465971.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA
    GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94789
    Clone ID Related Accession (Same CDS sequence) XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
    Accession Version XM_015465974.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA
    GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321460.1
    CDS375..5693
    Translation

    Target ORF information:

    RefSeq Version XM_015465974.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465974.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA
    GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94789
    Clone ID Related Accession (Same CDS sequence) XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
    Accession Version XM_015465970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit alpha-2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
    GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG
    ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG
    GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT
    ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC
    ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC
    ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC
    TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC
    ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA
    GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC
    ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG
    GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT
    GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC
    GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC
    ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA
    GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG
    GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT
    AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC
    GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC
    TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG
    ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA
    AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG
    CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC
    GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC
    AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC
    AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT
    GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC
    CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG
    CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG
    TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC
    CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG
    CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC
    CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT
    CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC
    AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA
    GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA
    GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC
    AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG
    AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC
    ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT
    GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC
    TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT
    GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC
    CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA
    TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC
    TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC
    CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG
    AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC
    ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG
    GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC
    CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA
    GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG
    TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA
    GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT
    CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG
    GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC
    ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC
    CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC
    CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG
    GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG
    AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG
    CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC
    CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA
    GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC
    CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC
    GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC
    ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC
    GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG
    AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC
    CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG
    GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC
    CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG
    GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT
    GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA
    GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT
    TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG
    CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT
    GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT
    GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG
    GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC
    GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC
    AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG
    GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC
    ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG
    CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC
    TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG
    GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG
    GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA
    GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.