CPS1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following CPS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa38271 NM_001192258.1
Latest version!
Bos taurus carbamoyl-phosphate synthase 1 (CPS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa38271 XM_025000627.1
Latest version!
Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa38271 XM_015458817.2
Latest version!
Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa38271 XM_010802252.3
Latest version!
Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa38271 XM_025000619.1
Latest version!
Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa38271 XM_010802252.2 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa38271 XM_015458817.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa38271
    Clone ID Related Accession (Same CDS sequence) XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
    Accession Version NM_001192258.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Jul 17, 2010 this sequence version replaced XM_587645.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.42606.1, SRR5571260.43926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145427, SAMN03145482 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179187.1
    CDS122..4624
    Translation

    Target ORF information:

    RefSeq Version NM_001192258.1
    Organism Bos taurus(cattle)
    Definition Bos taurus carbamoyl-phosphate synthase 1 (CPS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38271
    Clone ID Related Accession (Same CDS sequence) XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
    Accession Version XM_015458817.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314303.1
    CDS35..4537
    Translation

    Target ORF information:

    RefSeq Version XM_015458817.1
    Organism Bos taurus(cattle)
    Definition Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458817.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38271
    Clone ID Related Accession (Same CDS sequence) XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
    Accession Version XM_025000619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856387.1
    CDS286..4788
    Translation

    Target ORF information:

    RefSeq Version XM_025000619.1
    Organism Bos taurus(cattle)
    Definition Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38271
    Clone ID Related Accession (Same CDS sequence) XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
    Accession Version XM_010802252.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800554.1
    CDS397..4899
    Translation

    Target ORF information:

    RefSeq Version XM_010802252.3
    Organism Bos taurus(cattle)
    Definition Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802252.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38271
    Clone ID Related Accession (Same CDS sequence) XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
    Accession Version XM_025000627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856395.1
    CDS52..4554
    Translation

    Target ORF information:

    RefSeq Version XM_025000627.1
    Organism Bos taurus(cattle)
    Definition Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38271
    Clone ID Related Accession (Same CDS sequence) XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
    Accession Version XM_010802252.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800554.1
    CDS105..4607
    Translation

    Target ORF information:

    RefSeq Version XM_010802252.2
    Organism Bos taurus(cattle)
    Definition Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802252.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38271
    Clone ID Related Accession (Same CDS sequence) XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
    Accession Version XM_015458817.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314303.1
    CDS228..4730
    Translation

    Target ORF information:

    RefSeq Version XM_015458817.2
    Organism Bos taurus(cattle)
    Definition Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458817.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
    ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT
    GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC
    CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT
    GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT
    TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC
    CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA
    ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT
    GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG
    ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG
    GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA
    GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA
    ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG
    ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA
    GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA
    CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA
    AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT
    GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG
    GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT
    GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA
    TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC
    CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG
    CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA
    TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT
    ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA
    ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT
    GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT
    CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT
    AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA
    GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG
    AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC
    CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG
    ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC
    TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT
    TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA
    GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA
    AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC
    AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG
    ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA
    GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC
    CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC
    TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC
    CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG
    AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT
    CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT
    CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG
    TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT
    GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG
    GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT
    GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC
    GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT
    GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC
    AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA
    ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT
    GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA
    GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC
    AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC
    TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT
    AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA
    ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT
    CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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