COL4A5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL4A5
Entrez Gene ID	511602
Full Name	collagen type IV alpha 5 chain
General protein information	Preferred Names collagen type IV alpha 5 chain Names collagen alpha-5(IV) chain collagen, type IV, alpha 5
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024988380.1	XP_024844148.1	collagen alpha-5(IV) chain isoform X2
XM_002699860.4	XP_002699906.1	collagen alpha-5(IV) chain isoform X1
XM_001790133.6	XP_001790185.2	collagen alpha-5(IV) chain isoform X1
XM_002699862.4	XP_002699908.1	collagen alpha-5(IV) chain isoform X2
XM_002699862.5	XP_002699908.1	collagen alpha-5(IV) chain isoform X3
XM_002699860.3	XP_002699906.1	collagen alpha-5(IV) chain isoform X1
XM_001790134.6	XP_001790186.2	collagen alpha-5(IV) chain isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL4A5	XP_002699906.1
Canis lupus familiaris (dog)	COL4A5	NP_001002979.1
	COL4A5	XP_001098546.1

Gene Ontology

The following COL4A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238554	XM_024988380.1 Latest version!	Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa35387	XM_002699860.4 Latest version!	Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa35387	XM_001790133.6 Latest version!	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa35388	XM_001790134.6 Latest version!	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa35388	XM_002699862.5 Latest version!	Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa35388	XM_002699862.4	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa35387	XM_002699860.3	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL4A5 ( XM_024988380.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844148.1 Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

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CloneID	OBa238554
Clone ID Related Accession (Same CDS sequence)	XM_024988380.1
Accession Version	XM_024988380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGCTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATTGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GTCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844148.1
CDS	335..5401
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLSGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGVQGGAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVSGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEIGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGLAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGAKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPRGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGFQGPPGPEGPRGLPGSGAIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_024988380.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988380.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGCTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATTGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GTCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_002699860.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699906.1 Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

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CloneID	OBa35387
Clone ID Related Accession (Same CDS sequence)	XM_002699860.4 , XM_001790133.6 , XM_002699860.3
Accession Version	XM_002699860.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699860.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699906.1
CDS	373..5448
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLSGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGVQGGAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVSGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEIGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGLAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGAKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPRGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGFQGPPGPEGPRGLPGSGAIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_002699860.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699860.4

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_001790133.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001790185.2 Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35387
Clone ID Related Accession (Same CDS sequence)	XM_002699860.4 , XM_001790133.6 , XM_002699860.3
Accession Version	XM_001790133.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001790133.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001790185.2
CDS	31..5106
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLSGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGVQGGAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVSGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEIGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGLAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGAKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPRGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGFQGPPGPEGPRGLPGSGAIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_001790133.6
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001790133.6

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_002699860.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699906.1 Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.

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CloneID	OBa35387
Clone ID Related Accession (Same CDS sequence)	XM_002699860.4 , XM_001790133.6 , XM_002699860.3
Accession Version	XM_002699860.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 5, 2013 this sequence version replaced XM_002699860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699906.1
CDS	31..5106
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLSGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGVQGGAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVSGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEIGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGLAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGAKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPRGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGFQGPPGPEGPRGLPGSGAIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_002699860.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699860.3

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_002699862.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699908.1 Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

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CloneID	OBa35388
Clone ID Related Accession (Same CDS sequence)	XM_002699862.4 , XM_002699862.5 , XM_001790134.6
Accession Version	XM_002699862.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002699862.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699908.1
CDS	31..5088
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLSGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGVQGGAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVSGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEIGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGLAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGAKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPRGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPPGPEGPRGLPGSGAIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_002699862.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699862.4

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_002699862.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699908.1 Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

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CloneID	OBa35388
Clone ID Related Accession (Same CDS sequence)	XM_002699862.4 , XM_002699862.5 , XM_001790134.6
Accession Version	XM_002699862.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699862.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699908.1
CDS	335..5392
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLSGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGVQGGAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVSGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEIGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGLAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGAKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPRGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPPGPEGPRGLPGSGAIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_002699862.5
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699862.5

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_001790134.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001790186.2 Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

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CloneID	OBa35388
Clone ID Related Accession (Same CDS sequence)	XM_002699862.4 , XM_002699862.5 , XM_001790134.6
Accession Version	XM_001790134.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001790134.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001790186.2
CDS	31..5088
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLSGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGVQGGAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVSGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEIGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGLAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGAKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPRGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPPGPEGPRGLPGSGAIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_001790134.6
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001790134.6

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL4A5 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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