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NELFB cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol NELFB
Entrez Gene ID 512799
Full Name negative elongation factor complex member B
Synonyms COBRA1
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_024999770.1 XP_024855538.1 negative elongation factor B
XM_002691696.4 XP_002691742.4 negative elongation factor B
XM_590381.8 XP_590381.8 negative elongation factor B

Pathways from BioSystems


Homologies


COBRA1 XP_001087434.1
Rattus norvegicus (Norway rat) Nelfb NP_001101287.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012109 XP_320419.3
Homo sapiens (human) NELFB NP_056271.2
Bos taurus (cattle) NELFB XP_002691742.3
Xenopus tropicalis (tropical clawed frog) nelfb NP_001011448.1
Mus musculus (house mouse) Nelfb NP_067368.2
Gallus gallus (chicken) COBRA1 NP_001006202.1
Pan troglodytes (chimpanzee) COBRA1 XP_001136264.3
Canis lupus familiaris (dog) NELFB XP_548351.2
Danio rerio (zebrafish) nelfb NP_956462.1
Drosophila melanogaster (fruit fly) NELF-B NP_572402.1

Gene Ontology

The following NELFB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221084 XM_024999770.1
Latest version!		 Bos taurus negative elongation factor complex member B (NELFB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OBa48434 XM_002691696.4
Latest version!		 Bos taurus negative elongation factor complex member B (NELFB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $510.30
$729.00
OBa48434 XM_590381.8
Latest version!		 Bos taurus negative elongation factor complex member B (NELFB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa221084
Clone ID Related Accession (Same CDS sequence) XM_024999770.1
Accession Version XM_024999770.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product negative elongation factor B
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 11% of CDS bases ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCGGATGC CCCCACCTAG CTGTGCCAGC CAGTGGAGGG GTCCAGCTGC TGGACTCCCT 
AAGGGTTGGG CCGCTTGCCG AGCTGAGGAG CCAACTGTTG GGCTGCCCAA AGACCCTGTG 
AGCTCAGCTA GGGTCGTGCA GGCCAGGCTG CAGCCAACAG ATGCCCTGGG CTTCACCGGG 
AGGCCCTGCC CCACCCACAG CAGCGGTCTG GCGGGGCCGC GCAGGAGGGC GCGGCGGGCC 
CGGTGGGCGG AGCTTCGCCG GCGACCCGTG CGTCATCCGG GCACCGCCTT CGCGCTGTTT 
CTGCCGGGCG CGGGACGCGG CGCTCCTCCC GGGAACCCCT GCGCGACGGC GGCGGCGTTT 
CCGGGGAGCC CGTTGGCGGG CGGACCTGGC TGCGCGGAGC GGAAGTGGGC GGCTGCGGAG 
CGAGCGGAGC GGTGCTGCGG GTGGGACCTG GCCGAGCTGG AGGGCGCCGG GGAGCGGAGC 
GCGGGCGGCC CCCGAGGCCC GGGCGAGAGG ACCGCTGCAG GGCCCGTGGC GAGCGCGGCG 
GCGCCGGGGG AGCGCGGCGG GGATGGGGCG CCGGGCCGGG TGGGGGCCGG GGCCTCGGCC 
CTATTTGCGG GGCTGCAGGA CCTGGGTGTG GCCAACGGCG AGGACCTAAA GGAGACGCTG 
ACCAACTGCA CGGAGCCGCT CAAGGCCATC GAGCAGTTCC AGACAGAGAA CGGCGTGCTG 
CTGCCCTCCC TGCAGTCGGC CTTGCCGTTC TTGGACCTGC ACGGGACTCC GCGGCTGGAG 
TTCCACCAGT CGGTTTTTGA TGAGCTTCGG GACAAGCTGC TGGAGCGTGT GTCAGCCATC 
GCTTCCGAGG GGAAGGCCGA GGAAAGGTAT AAGAAACTGG AGGACCTCCT GGAGAAGAGC 
TTTTCTCTGG TGAAGATGCC ATCTCTGCAG CCGGTGGTGA TGTGTGTCAT GAAACACCTG 
CCCAAGGTTC CTGAGAAGAA GCTGAAGCTG GTGATGGCTG ACAAGGAGCT GTACCGGGCC 
TGCGCAGTGG AGGTGAAGCG GCAGATTTGG CAGGACAACC AGGCGCTCTT TGGCGATGAG 
GTCTCCCCGC TGCTGAAGCA GTACATCGTG GAGAAGGAGA GCGCGCTCTT CAGCACGGAG 
CTCTCAGTCC TGCACAACTT TTTCAGCCCT TCCCCCAAGA CCAGGCGCCA GGGTGAGGTG 
GTGCAGAGGC TGACGAGGAT GGTGGGGCGC AACGTGAAGC TGTACGACAT GGTCCTGCAG 
TTCCTGCGCA CGCTCTTCCT GCGCACGCGC AATGTGCACT ACTGCACACT GCGCGCCGAG 
CTGCTCATGT CCCTGCACGA CCTGGACGTC GGCGACATCT GCTCCGTGGA CCCCTGCCAC 
AAGTTCACCT GGTGCCTGGA CGCTTGCATC CGAGAGCGGT TTGTGGACAG CAAGAGGGCC 
CGCGAGCTGC AGGGCTTCCT TGACGGAGTG AAGAAGGGGC AGGAGCAAGT TCTGGGGGAC 
CTATCCATGA TCCTGTGTGA CCCCTTCGCC ATCAACACCC TGTCTCTGAG CACCGTCAGG 
CACCTGCAGG AGCTGGTGGG CCAGGAGGTG CTACCCAGGG ACAGCCCGGA CCTCCTCCTG 
CTGCTCCGGC TGCTGGCGCT GGGCCAGGGT GCGTGGGACA TGATCGACAG CCAGGTCTTC 
AAGGAGCCCA AGATGGAGGT GGAGCTGGTC ACCAGGTTCC TGCCCATGCT CATGTCCTTC 
GTGGTGGACG ACCACACCTT CAACGTGGAT CAGAAGCTCC CAGCCGAGGA GAAGGCCCCG 
GTCACGTACC CAAACACGCT GCCGGAGAGC TTCACCAAGT TCCTGCAGGA GCAGCGCATG 
GCCTGCGAAG TGGGGCTGTA CTACGTGCTG CACATCACCA AGCAGAGGAA TAAGAACGCA 
CTGCTGCGCC TGCTGCCGGG GCTCGTGGAG ACCTTCGGCG ACCTGGCGTT CGGGGACATC 
TTCCTGCACC TGCTCACGGG CAGCCTGGCC CTGCTGGCCG ACGAGTTCGC CCTGGAGGAC 
TTCTGCAGCA GCCTCTTCGA CGGCTTCCTC CTCACCGCCT CCCCAAGGAA GGAGAGCGTG 
CAGCGGCACG TCCTGCGGCT CCTGCTGCAC CTGCACCACC GGGTGGCCCC GTCCAAGCTG 
GAGGCGCTGC AGAAGGCACT GGAGCCCACG GGCCAGAGCG GAGAGGCGGT GAAGGAGCTG 
TATTCCCAGC TTGGAGAGAA GCTCGAGCAG CTGGACCACC GGAAGCCTAG CCCAGCCCAG 
GCCCCAGAGA CACCAGCCCT GGAGCTGCCT CTGCCGGCTG TGCCCGCCCC GGCTGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024855538.1
CDS1..2343
Translation

Target ORF information:

RefSeq Version XM_024999770.1
Organism Bos taurus(cattle)
Definition Bos taurus negative elongation factor complex member B (NELFB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024999770.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCGGATGC CCCCACCTAG CTGTGCCAGC CAGTGGAGGG GTCCAGCTGC TGGACTCCCT 
AAGGGTTGGG CCGCTTGCCG AGCTGAGGAG CCAACTGTTG GGCTGCCCAA AGACCCTGTG 
AGCTCAGCTA GGGTCGTGCA GGCCAGGCTG CAGCCAACAG ATGCCCTGGG CTTCACCGGG 
AGGCCCTGCC CCACCCACAG CAGCGGTCTG GCGGGGCCGC GCAGGAGGGC GCGGCGGGCC 
CGGTGGGCGG AGCTTCGCCG GCGACCCGTG CGTCATCCGG GCACCGCCTT CGCGCTGTTT 
CTGCCGGGCG CGGGACGCGG CGCTCCTCCC GGGAACCCCT GCGCGACGGC GGCGGCGTTT 
CCGGGGAGCC CGTTGGCGGG CGGACCTGGC TGCGCGGAGC GGAAGTGGGC GGCTGCGGAG 
CGAGCGGAGC GGTGCTGCGG GTGGGACCTG GCCGAGCTGG AGGGCGCCGG GGAGCGGAGC 
GCGGGCGGCC CCCGAGGCCC GGGCGAGAGG ACCGCTGCAG GGCCCGTGGC GAGCGCGGCG 
GCGCCGGGGG AGCGCGGCGG GGATGGGGCG CCGGGCCGGG TGGGGGCCGG GGCCTCGGCC 
CTATTTGCGG GGCTGCAGGA CCTGGGTGTG GCCAACGGCG AGGACCTAAA GGAGACGCTG 
ACCAACTGCA CGGAGCCGCT CAAGGCCATC GAGCAGTTCC AGACAGAGAA CGGCGTGCTG 
CTGCCCTCCC TGCAGTCGGC CTTGCCGTTC TTGGACCTGC ACGGGACTCC GCGGCTGGAG 
TTCCACCAGT CGGTTTTTGA TGAGCTTCGG GACAAGCTGC TGGAGCGTGT GTCAGCCATC 
GCTTCCGAGG GGAAGGCCGA GGAAAGGTAT AAGAAACTGG AGGACCTCCT GGAGAAGAGC 
TTTTCTCTGG TGAAGATGCC ATCTCTGCAG CCGGTGGTGA TGTGTGTCAT GAAACACCTG 
CCCAAGGTTC CTGAGAAGAA GCTGAAGCTG GTGATGGCTG ACAAGGAGCT GTACCGGGCC 
TGCGCAGTGG AGGTGAAGCG GCAGATTTGG CAGGACAACC AGGCGCTCTT TGGCGATGAG 
GTCTCCCCGC TGCTGAAGCA GTACATCGTG GAGAAGGAGA GCGCGCTCTT CAGCACGGAG 
CTCTCAGTCC TGCACAACTT TTTCAGCCCT TCCCCCAAGA CCAGGCGCCA GGGTGAGGTG 
GTGCAGAGGC TGACGAGGAT GGTGGGGCGC AACGTGAAGC TGTACGACAT GGTCCTGCAG 
TTCCTGCGCA CGCTCTTCCT GCGCACGCGC AATGTGCACT ACTGCACACT GCGCGCCGAG 
CTGCTCATGT CCCTGCACGA CCTGGACGTC GGCGACATCT GCTCCGTGGA CCCCTGCCAC 
AAGTTCACCT GGTGCCTGGA CGCTTGCATC CGAGAGCGGT TTGTGGACAG CAAGAGGGCC 
CGCGAGCTGC AGGGCTTCCT TGACGGAGTG AAGAAGGGGC AGGAGCAAGT TCTGGGGGAC 
CTATCCATGA TCCTGTGTGA CCCCTTCGCC ATCAACACCC TGTCTCTGAG CACCGTCAGG 
CACCTGCAGG AGCTGGTGGG CCAGGAGGTG CTACCCAGGG ACAGCCCGGA CCTCCTCCTG 
CTGCTCCGGC TGCTGGCGCT GGGCCAGGGT GCGTGGGACA TGATCGACAG CCAGGTCTTC 
AAGGAGCCCA AGATGGAGGT GGAGCTGGTC ACCAGGTTCC TGCCCATGCT CATGTCCTTC 
GTGGTGGACG ACCACACCTT CAACGTGGAT CAGAAGCTCC CAGCCGAGGA GAAGGCCCCG 
GTCACGTACC CAAACACGCT GCCGGAGAGC TTCACCAAGT TCCTGCAGGA GCAGCGCATG 
GCCTGCGAAG TGGGGCTGTA CTACGTGCTG CACATCACCA AGCAGAGGAA TAAGAACGCA 
CTGCTGCGCC TGCTGCCGGG GCTCGTGGAG ACCTTCGGCG ACCTGGCGTT CGGGGACATC 
TTCCTGCACC TGCTCACGGG CAGCCTGGCC CTGCTGGCCG ACGAGTTCGC CCTGGAGGAC 
TTCTGCAGCA GCCTCTTCGA CGGCTTCCTC CTCACCGCCT CCCCAAGGAA GGAGAGCGTG 
CAGCGGCACG TCCTGCGGCT CCTGCTGCAC CTGCACCACC GGGTGGCCCC GTCCAAGCTG 
GAGGCGCTGC AGAAGGCACT GGAGCCCACG GGCCAGAGCG GAGAGGCGGT GAAGGAGCTG 
TATTCCCAGC TTGGAGAGAA GCTCGAGCAG CTGGACCACC GGAAGCCTAG CCCAGCCCAG 
GCCCCAGAGA CACCAGCCCT GGAGCTGCCT CTGCCGGCTG TGCCCGCCCC GGCTGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa48434
Clone ID Related Accession (Same CDS sequence) XM_002691696.4 , XM_590381.8
Accession Version XM_002691696.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product negative elongation factor B
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104304.1) and transcript sequence (BC114764.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002691696.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases assembly gap :: added 273 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCGGATGC CCCCACCTAG CTGTGCCAGC CAGTGGAGGG GTCCAGCTGC TGGACTCCCT 
AAGGGTTGGG CCGCTTGCCG AGCTGAGGAG CCAACTGTTG GGCTGCCCAA AGACCCTGTG 
AGCTCAGCTA GGGTCGTGCA GGCCAGGCTG CAGCCAACAG ATGCCCTGGG CTTCACCGGG 
AGGCCCTGCC CCACCCACAG CAGCGGTCTG CTCAACCCTT TCCACAGCAT CAGTTCTGAA 
TCTCCTGAAG CAGGCCAACC CGCCGGTGGC AACGGCCCCC GGTGTTCCAG CCCAGCTCCG 
GTGCCTGCTT CCCAGGGGCC CGCTCGCCAA CCGCGTTCTG GTCGGCGACA GGAAGCTGGC 
CCCGGGAGGC TAGGCGGGGC CGCGCAGAGG GCGCGGCGGG CCCGGTGGGC GGAGCTTCGC 
CGGCGACCCG TGCGTCATCC GGGCACCGCC TTCGCGCTGT TTCTGCCGGG CGCGGGACGC 
GGCGCTCCTC CCGGGAACCC CTGCGCGACG GCGGCGGCGT TTCCGGGGAG CCCGTTGGCG 
GGCGGACCTG GCTGCGCGGA GCGGAAGTGG GCGGCTGCGG AGCGAGCGGA GCGGTGCTGC 
GGGTGGGACC TGGCCGAGCT GGAGGGCGCC GGGGAGCGGA GCGCGGGCGG CCCCCGAGGC 
CCGGGCGAGA GGACCGCTGC AGGGCCCGTG GCGAGCGCGG CGGCGCCGGG GGAGCGCGGC 
GGGGATGGGG CGCCGGGCCG GGTGGGGGCC GGGGCCTCGG CCCTATTTGC GGGGCTGCAG 
GACCTGGGTG TGGCCAACGG CGAGGACCTA AAGGAGACGC TGACCAACTG CACGGAGCCG 
CTCAAGGCCA TCGAGCAGTT CCAGACAGAG AACGGCGTGC TGCTGCCCTC CCTGCAGTCG 
GCCTTGCCGT TCTTGGACCT GCACGGGACT CCGCGGCTGG AGTTCCACCA GTCGGTTTTT 
GATGAGCTTC GGGACAAGCT GCTGGAGCGT GTGTCAGCCA TCGCTTCCGA GGGGAAGGCC 
GAGGAAAGGT ATAAGAAACT GGAGGACCTC CTGGAGAAGA GCTTTTCTCT GGTGAAGATG 
CCATCTCTGC AGCCGGTGGT GATGTGTGTC ATGAAACACC TGCCCAAGGT TCCTGAGAAG 
AAGCTGAAGC TGGTGATGGC TGACAAGGAG CTGTACCGGG CCTGCGCAGT GGAGGTGAAG 
CGGCAGATTT GGCAGGACAA CCAGGCGCTC TTTGGCGATG AGGTCTCCCC GCTGCTGAAG 
CAGTACATCG TGGAGAAGGA GAGCGCGCTC TTCAGCACGG AGCTCTCAGT CCTGCACAAC 
TTTTTCAGCC CTTCCCCCAA GACCAGGCGC CAGGGTGAGG TGGTGCAGAG GCTGACGAGG 
ATGGTGGGGC GCAACGTGAA GCTGTACGAC ATGGTCCTGC AGTTCCTGCG CACGCTCTTC 
CTGCGCACGC GCAATGTGCA CTACTGCACA CTGCGCGCCG AGCTGCTCAT GTCCCTGCAC 
GACCTGGACG TCGGCGACAT CTGCTCCGTG GACCCCTGCC ACAAGTTCAC CTGGTGCCTG 
GACGCTTGCA TCCGAGAGCG GTTTGTGGAC AGCAAGAGGG CCCGCGAGCT GCAGGGCTTC 
CTTGACGGAG TGAAGAAGGG GCAGGAGCAA GTTCTGGGGG ACCTATCCAT GATCCTGTGT 
GACCCCTTCG CCATCAACAC CCTGTCTCTG AGCACCGTCA GGCACCTGCA GGAGCTGGTG 
GGCCAGGAGG TGCTACCCAG GGACAGCCCG GACCTCCTCC TGCTGCTCCG GCTGCTGGCG 
CTGGGCCAGG GTGCGTGGGA CATGATCGAC AGCCAGGTCT TCAAGGAGCC CAAGATGGAG 
GTGGAGCTGG TCACCAGGTT CCTGCCCATG CTCATGTCCT TCGTGGTGGA CGACCACACC 
TTCAACGTGG ATCAGAAGCT CCCAGCCGAG GAGAAGGCCC CGGTCACGTA CCCAAACACG 
CTGCCGGAGA GCTTCACCAA GTTCCTGCAG GAGCAGCGCA TGGCCTGCGA AGTGGGGCTG 
TACTACGTGC TGCACATCAC CAAGCAGAGG AATAAGAACG CACTGCTGCG CCTGCTGCCG 
GGGCTCGTGG AGACCTTCGG CGACCTGGCG TTCGGGGACA TCTTCCTGCA CCTGCTCACG 
GGCAGCCTGG CCCTGCTGGC CGACGAGTTC GCCCTGGAGG ACTTCTGCAG CAGCCTCTTC 
GACGGCTTCC TCCTCACCGC CTCCCCAAGG AAGGAGAGCG TGCAGCGGCA CGTCCTGCGG 
CTCCTGCTGC ACCTGCACCA CCGGGTGGCC CCGTCCAAGC TGGAGGCGCT GCAGAAGGCA 
CTGGAGCCCA CGGGCCAGAG CGGAGAGGCG GTGAAGGAGC TGTATTCCCA GCTTGGAGAG 
AAGCTCGAGC AGCTGGACCA CCGGAAGCCT AGCCCAGCCC AGGCCCCAGA GACACCAGCC 
CTGGAGCTGC CTCTGCCGGC TGTGCCCGCC CCGGCTGTGC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002691742.4
CDS1..2505
Translation

Target ORF information:

RefSeq Version XM_002691696.4
Organism Bos taurus(cattle)
Definition Bos taurus negative elongation factor complex member B (NELFB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002691696.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCGGATGC CCCCACCTAG CTGTGCCAGC CAGTGGAGGG GTCCAGCTGC TGGACTCCCT 
AAGGGTTGGG CCGCTTGCCG AGCTGAGGAG CCAACTGTTG GGCTGCCCAA AGACCCTGTG 
AGCTCAGCTA GGGTCGTGCA GGCCAGGCTG CAGCCAACAG ATGCCCTGGG CTTCACCGGG 
AGGCCCTGCC CCACCCACAG CAGCGGTCTG CTCAACCCTT TCCACAGCAT CAGTTCTGAA 
TCTCCTGAAG CAGGCCAACC CGCCGGTGGC AACGGCCCCC GGTGTTCCAG CCCAGCTCCG 
GTGCCTGCTT CCCAGGGGCC CGCTCGCCAA CCGCGTTCTG GTCGGCGACA GGAAGCTGGC 
CCCGGGAGGC TAGGCGGGGC CGCGCAGAGG GCGCGGCGGG CCCGGTGGGC GGAGCTTCGC 
CGGCGACCCG TGCGTCATCC GGGCACCGCC TTCGCGCTGT TTCTGCCGGG CGCGGGACGC 
GGCGCTCCTC CCGGGAACCC CTGCGCGACG GCGGCGGCGT TTCCGGGGAG CCCGTTGGCG 
GGCGGACCTG GCTGCGCGGA GCGGAAGTGG GCGGCTGCGG AGCGAGCGGA GCGGTGCTGC 
GGGTGGGACC TGGCCGAGCT GGAGGGCGCC GGGGAGCGGA GCGCGGGCGG CCCCCGAGGC 
CCGGGCGAGA GGACCGCTGC AGGGCCCGTG GCGAGCGCGG CGGCGCCGGG GGAGCGCGGC 
GGGGATGGGG CGCCGGGCCG GGTGGGGGCC GGGGCCTCGG CCCTATTTGC GGGGCTGCAG 
GACCTGGGTG TGGCCAACGG CGAGGACCTA AAGGAGACGC TGACCAACTG CACGGAGCCG 
CTCAAGGCCA TCGAGCAGTT CCAGACAGAG AACGGCGTGC TGCTGCCCTC CCTGCAGTCG 
GCCTTGCCGT TCTTGGACCT GCACGGGACT CCGCGGCTGG AGTTCCACCA GTCGGTTTTT 
GATGAGCTTC GGGACAAGCT GCTGGAGCGT GTGTCAGCCA TCGCTTCCGA GGGGAAGGCC 
GAGGAAAGGT ATAAGAAACT GGAGGACCTC CTGGAGAAGA GCTTTTCTCT GGTGAAGATG 
CCATCTCTGC AGCCGGTGGT GATGTGTGTC ATGAAACACC TGCCCAAGGT TCCTGAGAAG 
AAGCTGAAGC TGGTGATGGC TGACAAGGAG CTGTACCGGG CCTGCGCAGT GGAGGTGAAG 
CGGCAGATTT GGCAGGACAA CCAGGCGCTC TTTGGCGATG AGGTCTCCCC GCTGCTGAAG 
CAGTACATCG TGGAGAAGGA GAGCGCGCTC TTCAGCACGG AGCTCTCAGT CCTGCACAAC 
TTTTTCAGCC CTTCCCCCAA GACCAGGCGC CAGGGTGAGG TGGTGCAGAG GCTGACGAGG 
ATGGTGGGGC GCAACGTGAA GCTGTACGAC ATGGTCCTGC AGTTCCTGCG CACGCTCTTC 
CTGCGCACGC GCAATGTGCA CTACTGCACA CTGCGCGCCG AGCTGCTCAT GTCCCTGCAC 
GACCTGGACG TCGGCGACAT CTGCTCCGTG GACCCCTGCC ACAAGTTCAC CTGGTGCCTG 
GACGCTTGCA TCCGAGAGCG GTTTGTGGAC AGCAAGAGGG CCCGCGAGCT GCAGGGCTTC 
CTTGACGGAG TGAAGAAGGG GCAGGAGCAA GTTCTGGGGG ACCTATCCAT GATCCTGTGT 
GACCCCTTCG CCATCAACAC CCTGTCTCTG AGCACCGTCA GGCACCTGCA GGAGCTGGTG 
GGCCAGGAGG TGCTACCCAG GGACAGCCCG GACCTCCTCC TGCTGCTCCG GCTGCTGGCG 
CTGGGCCAGG GTGCGTGGGA CATGATCGAC AGCCAGGTCT TCAAGGAGCC CAAGATGGAG 
GTGGAGCTGG TCACCAGGTT CCTGCCCATG CTCATGTCCT TCGTGGTGGA CGACCACACC 
TTCAACGTGG ATCAGAAGCT CCCAGCCGAG GAGAAGGCCC CGGTCACGTA CCCAAACACG 
CTGCCGGAGA GCTTCACCAA GTTCCTGCAG GAGCAGCGCA TGGCCTGCGA AGTGGGGCTG 
TACTACGTGC TGCACATCAC CAAGCAGAGG AATAAGAACG CACTGCTGCG CCTGCTGCCG 
GGGCTCGTGG AGACCTTCGG CGACCTGGCG TTCGGGGACA TCTTCCTGCA CCTGCTCACG 
GGCAGCCTGG CCCTGCTGGC CGACGAGTTC GCCCTGGAGG ACTTCTGCAG CAGCCTCTTC 
GACGGCTTCC TCCTCACCGC CTCCCCAAGG AAGGAGAGCG TGCAGCGGCA CGTCCTGCGG 
CTCCTGCTGC ACCTGCACCA CCGGGTGGCC CCGTCCAAGC TGGAGGCGCT GCAGAAGGCA 
CTGGAGCCCA CGGGCCAGAG CGGAGAGGCG GTGAAGGAGC TGTATTCCCA GCTTGGAGAG 
AAGCTCGAGC AGCTGGACCA CCGGAAGCCT AGCCCAGCCC AGGCCCCAGA GACACCAGCC 
CTGGAGCTGC CTCTGCCGGC TGTGCCCGCC CCGGCTGTGC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa48434
Clone ID Related Accession (Same CDS sequence) XM_002691696.4 , XM_590381.8
Accession Version XM_590381.8 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product negative elongation factor B
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645930.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_590381.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCGGATGC CCCCACCTAG CTGTGCCAGC CAGTGGAGGG GTCCAGCTGC TGGACTCCCT 
AAGGGTTGGG CCGCTTGCCG AGCTGAGGAG CCAACTGTTG GGCTGCCCAA AGACCCTGTG 
AGCTCAGCTA GGGTCGTGCA GGCCAGGCTG CAGCCAACAG ATGCCCTGGG CTTCACCGGG 
AGGCCCTGCC CCACCCACAG CAGCGGTCTG CTCAACCCTT TCCACAGCAT CAGTTCTGAA 
TCTCCTGAAG CAGGCCAACC CGCCGGTGGC AACGGCCCCC GGTGTTCCAG CCCAGCTCCG 
GTGCCTGCTT CCCAGGGGCC CGCTCGCCAA CCGCGTTCTG GTCGGCGACA GGAAGCTGGC 
CCCGGGAGGC TAGGCGGGGC CGCGCAGAGG GCGCGGCGGG CCCGGTGGGC GGAGCTTCGC 
CGGCGACCCG TGCGTCATCC GGGCACCGCC TTCGCGCTGT TTCTGCCGGG CGCGGGACGC 
GGCGCTCCTC CCGGGAACCC CTGCGCGACG GCGGCGGCGT TTCCGGGGAG CCCGTTGGCG 
GGCGGACCTG GCTGCGCGGA GCGGAAGTGG GCGGCTGCGG AGCGAGCGGA GCGGTGCTGC 
GGGTGGGACC TGGCCGAGCT GGAGGGCGCC GGGGAGCGGA GCGCGGGCGG CCCCCGAGGC 
CCGGGCGAGA GGACCGCTGC AGGGCCCGTG GCGAGCGCGG CGGCGCCGGG GGAGCGCGGC 
GGGGATGGGG CGCCGGGCCG GGTGGGGGCC GGGGCCTCGG CCCTATTTGC GGGGCTGCAG 
GACCTGGGTG TGGCCAACGG CGAGGACCTA AAGGAGACGC TGACCAACTG CACGGAGCCG 
CTCAAGGCCA TCGAGCAGTT CCAGACAGAG AACGGCGTGC TGCTGCCCTC CCTGCAGTCG 
GCCTTGCCGT TCTTGGACCT GCACGGGACT CCGCGGCTGG AGTTCCACCA GTCGGTTTTT 
GATGAGCTTC GGGACAAGCT GCTGGAGCGT GTGTCAGCCA TCGCTTCCGA GGGGAAGGCC 
GAGGAAAGGT ATAAGAAACT GGAGGACCTC CTGGAGAAGA GCTTTTCTCT GGTGAAGATG 
CCATCTCTGC AGCCGGTGGT GATGTGTGTC ATGAAACACC TGCCCAAGGT TCCTGAGAAG 
AAGCTGAAGC TGGTGATGGC TGACAAGGAG CTGTACCGGG CCTGCGCAGT GGAGGTGAAG 
CGGCAGATTT GGCAGGACAA CCAGGCGCTC TTTGGCGATG AGGTCTCCCC GCTGCTGAAG 
CAGTACATCG TGGAGAAGGA GAGCGCGCTC TTCAGCACGG AGCTCTCAGT CCTGCACAAC 
TTTTTCAGCC CTTCCCCCAA GACCAGGCGC CAGGGTGAGG TGGTGCAGAG GCTGACGAGG 
ATGGTGGGGC GCAACGTGAA GCTGTACGAC ATGGTCCTGC AGTTCCTGCG CACGCTCTTC 
CTGCGCACGC GCAATGTGCA CTACTGCACA CTGCGCGCCG AGCTGCTCAT GTCCCTGCAC 
GACCTGGACG TCGGCGACAT CTGCTCCGTG GACCCCTGCC ACAAGTTCAC CTGGTGCCTG 
GACGCTTGCA TCCGAGAGCG GTTTGTGGAC AGCAAGAGGG CCCGCGAGCT GCAGGGCTTC 
CTTGACGGAG TGAAGAAGGG GCAGGAGCAA GTTCTGGGGG ACCTATCCAT GATCCTGTGT 
GACCCCTTCG CCATCAACAC CCTGTCTCTG AGCACCGTCA GGCACCTGCA GGAGCTGGTG 
GGCCAGGAGG TGCTACCCAG GGACAGCCCG GACCTCCTCC TGCTGCTCCG GCTGCTGGCG 
CTGGGCCAGG GTGCGTGGGA CATGATCGAC AGCCAGGTCT TCAAGGAGCC CAAGATGGAG 
GTGGAGCTGG TCACCAGGTT CCTGCCCATG CTCATGTCCT TCGTGGTGGA CGACCACACC 
TTCAACGTGG ATCAGAAGCT CCCAGCCGAG GAGAAGGCCC CGGTCACGTA CCCAAACACG 
CTGCCGGAGA GCTTCACCAA GTTCCTGCAG GAGCAGCGCA TGGCCTGCGA AGTGGGGCTG 
TACTACGTGC TGCACATCAC CAAGCAGAGG AATAAGAACG CACTGCTGCG CCTGCTGCCG 
GGGCTCGTGG AGACCTTCGG CGACCTGGCG TTCGGGGACA TCTTCCTGCA CCTGCTCACG 
GGCAGCCTGG CCCTGCTGGC CGACGAGTTC GCCCTGGAGG ACTTCTGCAG CAGCCTCTTC 
GACGGCTTCC TCCTCACCGC CTCCCCAAGG AAGGAGAGCG TGCAGCGGCA CGTCCTGCGG 
CTCCTGCTGC ACCTGCACCA CCGGGTGGCC CCGTCCAAGC TGGAGGCGCT GCAGAAGGCA 
CTGGAGCCCA CGGGCCAGAG CGGAGAGGCG GTGAAGGAGC TGTATTCCCA GCTTGGAGAG 
AAGCTCGAGC AGCTGGACCA CCGGAAGCCT AGCCCAGCCC AGGCCCCAGA GACACCAGCC 
CTGGAGCTGC CTCTGCCGGC TGTGCCCGCC CCGGCTGTGC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_590381.8
CDS1..2505
Translation

Target ORF information:

RefSeq Version XM_590381.8
Organism Bos taurus(cattle)
Definition Bos taurus negative elongation factor complex member B (NELFB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_590381.8

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCGGATGC CCCCACCTAG CTGTGCCAGC CAGTGGAGGG GTCCAGCTGC TGGACTCCCT 
AAGGGTTGGG CCGCTTGCCG AGCTGAGGAG CCAACTGTTG GGCTGCCCAA AGACCCTGTG 
AGCTCAGCTA GGGTCGTGCA GGCCAGGCTG CAGCCAACAG ATGCCCTGGG CTTCACCGGG 
AGGCCCTGCC CCACCCACAG CAGCGGTCTG CTCAACCCTT TCCACAGCAT CAGTTCTGAA 
TCTCCTGAAG CAGGCCAACC CGCCGGTGGC AACGGCCCCC GGTGTTCCAG CCCAGCTCCG 
GTGCCTGCTT CCCAGGGGCC CGCTCGCCAA CCGCGTTCTG GTCGGCGACA GGAAGCTGGC 
CCCGGGAGGC TAGGCGGGGC CGCGCAGAGG GCGCGGCGGG CCCGGTGGGC GGAGCTTCGC 
CGGCGACCCG TGCGTCATCC GGGCACCGCC TTCGCGCTGT TTCTGCCGGG CGCGGGACGC 
GGCGCTCCTC CCGGGAACCC CTGCGCGACG GCGGCGGCGT TTCCGGGGAG CCCGTTGGCG 
GGCGGACCTG GCTGCGCGGA GCGGAAGTGG GCGGCTGCGG AGCGAGCGGA GCGGTGCTGC 
GGGTGGGACC TGGCCGAGCT GGAGGGCGCC GGGGAGCGGA GCGCGGGCGG CCCCCGAGGC 
CCGGGCGAGA GGACCGCTGC AGGGCCCGTG GCGAGCGCGG CGGCGCCGGG GGAGCGCGGC 
GGGGATGGGG CGCCGGGCCG GGTGGGGGCC GGGGCCTCGG CCCTATTTGC GGGGCTGCAG 
GACCTGGGTG TGGCCAACGG CGAGGACCTA AAGGAGACGC TGACCAACTG CACGGAGCCG 
CTCAAGGCCA TCGAGCAGTT CCAGACAGAG AACGGCGTGC TGCTGCCCTC CCTGCAGTCG 
GCCTTGCCGT TCTTGGACCT GCACGGGACT CCGCGGCTGG AGTTCCACCA GTCGGTTTTT 
GATGAGCTTC GGGACAAGCT GCTGGAGCGT GTGTCAGCCA TCGCTTCCGA GGGGAAGGCC 
GAGGAAAGGT ATAAGAAACT GGAGGACCTC CTGGAGAAGA GCTTTTCTCT GGTGAAGATG 
CCATCTCTGC AGCCGGTGGT GATGTGTGTC ATGAAACACC TGCCCAAGGT TCCTGAGAAG 
AAGCTGAAGC TGGTGATGGC TGACAAGGAG CTGTACCGGG CCTGCGCAGT GGAGGTGAAG 
CGGCAGATTT GGCAGGACAA CCAGGCGCTC TTTGGCGATG AGGTCTCCCC GCTGCTGAAG 
CAGTACATCG TGGAGAAGGA GAGCGCGCTC TTCAGCACGG AGCTCTCAGT CCTGCACAAC 
TTTTTCAGCC CTTCCCCCAA GACCAGGCGC CAGGGTGAGG TGGTGCAGAG GCTGACGAGG 
ATGGTGGGGC GCAACGTGAA GCTGTACGAC ATGGTCCTGC AGTTCCTGCG CACGCTCTTC 
CTGCGCACGC GCAATGTGCA CTACTGCACA CTGCGCGCCG AGCTGCTCAT GTCCCTGCAC 
GACCTGGACG TCGGCGACAT CTGCTCCGTG GACCCCTGCC ACAAGTTCAC CTGGTGCCTG 
GACGCTTGCA TCCGAGAGCG GTTTGTGGAC AGCAAGAGGG CCCGCGAGCT GCAGGGCTTC 
CTTGACGGAG TGAAGAAGGG GCAGGAGCAA GTTCTGGGGG ACCTATCCAT GATCCTGTGT 
GACCCCTTCG CCATCAACAC CCTGTCTCTG AGCACCGTCA GGCACCTGCA GGAGCTGGTG 
GGCCAGGAGG TGCTACCCAG GGACAGCCCG GACCTCCTCC TGCTGCTCCG GCTGCTGGCG 
CTGGGCCAGG GTGCGTGGGA CATGATCGAC AGCCAGGTCT TCAAGGAGCC CAAGATGGAG 
GTGGAGCTGG TCACCAGGTT CCTGCCCATG CTCATGTCCT TCGTGGTGGA CGACCACACC 
TTCAACGTGG ATCAGAAGCT CCCAGCCGAG GAGAAGGCCC CGGTCACGTA CCCAAACACG 
CTGCCGGAGA GCTTCACCAA GTTCCTGCAG GAGCAGCGCA TGGCCTGCGA AGTGGGGCTG 
TACTACGTGC TGCACATCAC CAAGCAGAGG AATAAGAACG CACTGCTGCG CCTGCTGCCG 
GGGCTCGTGG AGACCTTCGG CGACCTGGCG TTCGGGGACA TCTTCCTGCA CCTGCTCACG 
GGCAGCCTGG CCCTGCTGGC CGACGAGTTC GCCCTGGAGG ACTTCTGCAG CAGCCTCTTC 
GACGGCTTCC TCCTCACCGC CTCCCCAAGG AAGGAGAGCG TGCAGCGGCA CGTCCTGCGG 
CTCCTGCTGC ACCTGCACCA CCGGGTGGCC CCGTCCAAGC TGGAGGCGCT GCAGAAGGCA 
CTGGAGCCCA CGGGCCAGAG CGGAGAGGCG GTGAAGGAGC TGTATTCCCA GCTTGGAGAG 
AAGCTCGAGC AGCTGGACCA CCGGAAGCCT AGCCCAGCCC AGGCCCCAGA GACACCAGCC 
CTGGAGCTGC CTCTGCCGGC TGTGCCCGCC CCGGCTGTGC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed