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CARS cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol CARS
Entrez Gene ID 515715
Full Name cysteinyl-tRNA synthetase
General protein information
Preferred Names

cysteinyl-tRNA synthetase

Names

cysteine--tRNA ligase, cytoplasmic
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001103249.1 NP_001096719.1 cysteine--tRNA ligase, cytoplasmic
XM_015461344.1 XP_015316830.1 cysteine--tRNA ligase, cytoplasmic isoform X1
XM_024987144.1 XP_024842912.1 cysteine--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems


Homologies


CARS XP_001096302.1
Oryza sativa (rice) Os09g0556500 NP_001063899.1
Homo sapiens (human) CARS NP_001014437.1
Rattus norvegicus (Norway rat) Cars NP_001099789.1
Drosophila melanogaster (fruit fly) Aats-cys NP_611087.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012283 XP_320253.2
Arabidopsis thaliana (thale cress) SYCO ARATH NP_565717.2
Mus musculus (house mouse) Cars NP_038770.3
Gallus gallus (chicken) CARS NP_001012601.1
Caenorhabditis elegans (roundworm) cars-1 NP_491014.1
Saccharomyces cerevisiae (baker's yeast) YNL247W NP_014152.1
Kluyveromyces lactis KLLA0C03025g XP_452332.1
Pan troglodytes (chimpanzee) CARS XP_003312912.1
Canis lupus familiaris (dog) CARS XP_851224.1
Bos taurus (cattle) CARS NP_001096719.1
Xenopus tropicalis (tropical clawed frog) cars NP_001011365.1
Danio rerio (zebrafish) cars NP_001112372.1
Schizosaccharomyces pombe (fission yeast) SPAC29E6.06c NP_594564.1

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231971 XM_024987144.1
Latest version!		 Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OBa39791 NM_001103249.1
Latest version!		 Bos taurus cysteinyl-tRNA synthetase 1 (CARS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $426.30
$609.00
OBa89150 XM_015461344.1
Latest version!		 Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $426.30
$609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa231971
Clone ID Related Accession (Same CDS sequence) XM_024987144.1
Accession Version XM_024987144.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product cysteine--tRNA ligase, cytoplasmic isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGGGT CTACCACGGA GCACGCAGGC GGAGGGCGGC GTGCACAGCC CACATGGTCT 
CCTCCAGCTG GGTCTGAGCC ATGCCAGCTG CGCCTGTACA ACAGCCTGAC ACGGAGGAAG 
GACGTGTTTG TGCCGCAAGA CGGAAGGAGG GTGACATGGT ACTGCTGCGG GCCCACCGTC 
TACGACGCCT CGCACATGGG GCACGCCAGG TCCTACATCT CCTTCGATAT CCTGAGAAGA 
GTGCTGAGGG ATTACTTCAA ATTTGACGTC TTCTACTGCA TGAACATCAC GGACATTGAC 
GATAAGATCA TCAAGAGGGC CCGGCAGAAC TTCCTGTTGG AGCGGTACCG GGAGACGCAG 
CCCCAGGCAG CGCAGCTGCT GGAGGACGTG CGAGCCGCCC TGCAGCCGTT CTCCGCGAAG 
CTCCAGGAGA CTGTGGACCC GGACAAGAAG CAGATGCTGG AACGCCTGCA GCACGCGGTG 
GCGCAGGCCG CGGAGCCGCT GGAGGGCGCG CTGCGGGCCG GGCAGGCCGG GCAGGAGCTG 
GACGGCCGCG TGCAGGCGTT GCTGGAAGAA GCAAAGGATG TGCTTTCTGA CTGGCTGGAT 
TCCATGCACG GCAGTGAGGT GACGGACAAT TCTATTTTCT CTGAACTGCC CAAATTCTGG 
GAGGCGGAGT TCCACAAAGA CATGGAAGCC CTGAACGTCC TCCCTCCGGA CGTCCTGACC 
CGGGTTAGTG AGTATGTGCC AGAGATCGTG GACTTTGTCC AGAAGATCGT GGACAACGGT 
TACGGGTATG CTTCAAACGG GTCTGTGTAC TTCGACACAG TCAAGTTTGC CAACACTGAG 
GGGCACAGCT ACGGAAAGCT GGTGCCCGAG GCCGTCGGGG ACCAGAAAGC CCTGCACGAG 
GGGGAAGGCG ACCTGAGCGT CTCTGCAGAG CGCCTGAGTG AGAAGCGGTC CCCCAATGAC 
TTTGCGCTCT GGAAGGCCTC CAAGCCCGGG GAGCCGTCCT GGCCCTGCCC CTGGGGCAAG 
GGGCGCCCCG GATGGCACAT CGAGTGCTCG GCCATGGCGG GCACCCTCCT GGGAGCCTCG 
ATGGACATCC ACGGAGGGGG CTTCGACCTC CGCTTCCCTC ACCACGACAA CGAGCTGGCG 
CAGTCAGAGG CCTACTTTGA GAACGACTGC TGGGTCAGGT ACTTCCTGCA CACAGGCCAC 
CTGACCATCG CGGGCTGCAA GATGTCCAAG TCGCTCAAGA ACTTCATCAC CATTAAAGAC 
GCCCTGAAGA AGCACTCAGC CCGCCAGCTG CGCCTGGCCT TCCTCATGCA CTCGTGGAAG 
GACACGCTGG ACTACTCGGC CAACACCATG GAGTCGGCGC TGCAGTACGA GAGGCTCCTC 
AATGAGTTTT TCTTAAACGT GAAAGACCTC CTCCGAGCCC CCGTGGAGGT GGCCGCTCAG 
TTTGAGAAGT GGGAGGACGA GGAAACGGAG CTGAACAAGA GCTTTTACAA GAAGAAGGCC 
GCGGTTCACC AGGCGCTCTG TGACAACATC GACACGCGCA CAGTCCTGGA GGAGATGCGG 
GCTCTGGTCG GCCAGTGCAA CCTCTACATG GCGGCCCGCA AGGCCGCCCG GAGGAGGCCC 
CACCGCGTCC TGCTGGAGAG CATCGCCCAC TACCTCACAC ACATGCTGAA GATCTTCGGG 
GCCATAGAAG AGGAAAGCTC CTTGGGTTTC CCCATCGGAG GAGCTGGAAC CAGCCTCAAT 
CTGGAGTCCA CGGTCATGCC CTACCTGCAG GTGCTGTCGG AGTTCCGGGA AGGGGTGCGG 
CGGATCGCCC GTGAGCACAG AGTGCCTGAG GTCCTCCAGC TGAGCGACGC CCTGCGGGAC 
AACGTCCTGC CCGAGCTGGG GGTGCGGCTG GAAGACCACG AAGGTCTGCC CACCGTGGTC 
AAGCTGGTGG ACAGAGACGC CTTACTGAGA GAGAGAGAAG ACAAGAAAAG GGCGGAAGAG 
GAGAAAAGGA AGAAGAAAGA GGAGGCTACC AGGAAGAGAC AGGAGCAGGA GGCAGCAAAG 
CTGGCCAAGA TGAAGATCCC ACCCAGTGAG ATGTTCTTAT CGGAAAGCGA CAAATACTCC 
AAGTTCGACG AAAATGGTCT GCCCACGCAC GACACCGAAG GCAAAGAGCT GAGCAAGGGG 
CAGGCCAAGA AGCTGCGGAA GCTGTTCGAG GCGCAGGAGA AGCTGCACTT AGAGTACCTG 
CAACTGGTTC AGAACGGGGG CCCCCCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024842912.1
CDS55..2304
Translation

Target ORF information:

RefSeq Version XM_024987144.1
Organism Bos taurus(cattle)
Definition Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024987144.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGGGT CTACCACGGA GCACGCAGGC GGAGGGCGGC GTGCACAGCC CACATGGTCT 
CCTCCAGCTG GGTCTGAGCC ATGCCAGCTG CGCCTGTACA ACAGCCTGAC ACGGAGGAAG 
GACGTGTTTG TGCCGCAAGA CGGAAGGAGG GTGACATGGT ACTGCTGCGG GCCCACCGTC 
TACGACGCCT CGCACATGGG GCACGCCAGG TCCTACATCT CCTTCGATAT CCTGAGAAGA 
GTGCTGAGGG ATTACTTCAA ATTTGACGTC TTCTACTGCA TGAACATCAC GGACATTGAC 
GATAAGATCA TCAAGAGGGC CCGGCAGAAC TTCCTGTTGG AGCGGTACCG GGAGACGCAG 
CCCCAGGCAG CGCAGCTGCT GGAGGACGTG CGAGCCGCCC TGCAGCCGTT CTCCGCGAAG 
CTCCAGGAGA CTGTGGACCC GGACAAGAAG CAGATGCTGG AACGCCTGCA GCACGCGGTG 
GCGCAGGCCG CGGAGCCGCT GGAGGGCGCG CTGCGGGCCG GGCAGGCCGG GCAGGAGCTG 
GACGGCCGCG TGCAGGCGTT GCTGGAAGAA GCAAAGGATG TGCTTTCTGA CTGGCTGGAT 
TCCATGCACG GCAGTGAGGT GACGGACAAT TCTATTTTCT CTGAACTGCC CAAATTCTGG 
GAGGCGGAGT TCCACAAAGA CATGGAAGCC CTGAACGTCC TCCCTCCGGA CGTCCTGACC 
CGGGTTAGTG AGTATGTGCC AGAGATCGTG GACTTTGTCC AGAAGATCGT GGACAACGGT 
TACGGGTATG CTTCAAACGG GTCTGTGTAC TTCGACACAG TCAAGTTTGC CAACACTGAG 
GGGCACAGCT ACGGAAAGCT GGTGCCCGAG GCCGTCGGGG ACCAGAAAGC CCTGCACGAG 
GGGGAAGGCG ACCTGAGCGT CTCTGCAGAG CGCCTGAGTG AGAAGCGGTC CCCCAATGAC 
TTTGCGCTCT GGAAGGCCTC CAAGCCCGGG GAGCCGTCCT GGCCCTGCCC CTGGGGCAAG 
GGGCGCCCCG GATGGCACAT CGAGTGCTCG GCCATGGCGG GCACCCTCCT GGGAGCCTCG 
ATGGACATCC ACGGAGGGGG CTTCGACCTC CGCTTCCCTC ACCACGACAA CGAGCTGGCG 
CAGTCAGAGG CCTACTTTGA GAACGACTGC TGGGTCAGGT ACTTCCTGCA CACAGGCCAC 
CTGACCATCG CGGGCTGCAA GATGTCCAAG TCGCTCAAGA ACTTCATCAC CATTAAAGAC 
GCCCTGAAGA AGCACTCAGC CCGCCAGCTG CGCCTGGCCT TCCTCATGCA CTCGTGGAAG 
GACACGCTGG ACTACTCGGC CAACACCATG GAGTCGGCGC TGCAGTACGA GAGGCTCCTC 
AATGAGTTTT TCTTAAACGT GAAAGACCTC CTCCGAGCCC CCGTGGAGGT GGCCGCTCAG 
TTTGAGAAGT GGGAGGACGA GGAAACGGAG CTGAACAAGA GCTTTTACAA GAAGAAGGCC 
GCGGTTCACC AGGCGCTCTG TGACAACATC GACACGCGCA CAGTCCTGGA GGAGATGCGG 
GCTCTGGTCG GCCAGTGCAA CCTCTACATG GCGGCCCGCA AGGCCGCCCG GAGGAGGCCC 
CACCGCGTCC TGCTGGAGAG CATCGCCCAC TACCTCACAC ACATGCTGAA GATCTTCGGG 
GCCATAGAAG AGGAAAGCTC CTTGGGTTTC CCCATCGGAG GAGCTGGAAC CAGCCTCAAT 
CTGGAGTCCA CGGTCATGCC CTACCTGCAG GTGCTGTCGG AGTTCCGGGA AGGGGTGCGG 
CGGATCGCCC GTGAGCACAG AGTGCCTGAG GTCCTCCAGC TGAGCGACGC CCTGCGGGAC 
AACGTCCTGC CCGAGCTGGG GGTGCGGCTG GAAGACCACG AAGGTCTGCC CACCGTGGTC 
AAGCTGGTGG ACAGAGACGC CTTACTGAGA GAGAGAGAAG ACAAGAAAAG GGCGGAAGAG 
GAGAAAAGGA AGAAGAAAGA GGAGGCTACC AGGAAGAGAC AGGAGCAGGA GGCAGCAAAG 
CTGGCCAAGA TGAAGATCCC ACCCAGTGAG ATGTTCTTAT CGGAAAGCGA CAAATACTCC 
AAGTTCGACG AAAATGGTCT GCCCACGCAC GACACCGAAG GCAAAGAGCT GAGCAAGGGG 
CAGGCCAAGA AGCTGCGGAA GCTGTTCGAG GCGCAGGAGA AGCTGCACTT AGAGTACCTG 
CAACTGGTTC AGAACGGGGG CCCCCCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa39791
Clone ID Related Accession (Same CDS sequence) NM_001103249.1
Accession Version NM_001103249.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-30
Organism Bos taurus(cattle)
Product cysteine--tRNA ligase, cytoplasmic
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114705.1. On Sep 13, 2007 this sequence version replaced XR_028643.1. ##Evidence-Data-START## Transcript exon combination :: BC114705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC 
TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC 
CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC 
CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAACAGGCG GAGGGCGGCG TGCACAGCCC 
ACATGGTCTC CTCCAGCTGG GTCTGAGCCA TGCCAGCTGC GCCTGTACAA CAGCCTGACA 
CGGAGGAAGG ACGTGTTTGT GCCGCAAGAC GGAAGGAGGG TGACATGGTA CTGCTGCGGG 
CCCACCGTCT ACGACGCCTC GCACATGGGG CACGCCAGGT CCTACATCTC CTTCGATATC 
TTGAGAAGAG TGCTGAGGGA TTACTTCAAA TTTGACGTCT TCTACTGCAT GAACATCACG 
GACATTGACG ATAAGATCAT CAAGAGGGCC CGGCAGAACT TCCTGTTGGA GCGGTACCGG 
GAGACGCAGC CCCAGGCAGC GCAGCTGCTG GAGGACGTGC GAGCCGCCCT GCAGCCGTTC 
TCCGCGAAGC TCCAGGAGAC TGTGGACCCG GACAAGAAGC AGATGCTGGA ACGCCTGCAG 
CACGCGGTGG CGCAGGCCGC GGAGCCGCTG GAGGGCGCGC TGCGGGCCGG GCAGGCCGGG 
CAGGAGCTGG ACGGCCGCGT GCAGGCGTTG CTGGAAGAAG CAAAGGATGT GCTTTCTGAC 
TGGCTGGATT CCATGCACGG CAGTGAGGTG ACGGACAATT CTATTTTCTC TGAACTGCCC 
AAATTCTGGG AGGCGGAGTT CCACAAAGAC ATGGAAGCCC TGAACGTCCT CCCTCCGGAC 
GTCCTGACCC GGGTTAGTGA GTATGTGCCA GAGATCGTGG ACTTTGTCCA GAAGATCGTG 
GACAACGGTT ACGGGTATGC TTCAAACGGG TCTGTGTACT TCGACACAGT CAAGTTTGCC 
AACACTGAGG GGCACAGCTA CGGAAAGCTG GTGCCCGAGG CCGTCGGGGA CCAGAAAGCC 
CTGCACGAGG GGGAAGGCGA CCTGAGCGTC TCTGCAGAGC GCCTGAGTGA GAAGCGGTCC 
CCCAATGACT TTGCGCTCTG GAAGGCCTCC AAGCCCGGGG AGCCGTCCTG GCCCTGCCCC 
TGGGGCAAGG GGCGCCCCGG ATGGCACATC GAGTGCTCGG CCATGGCGGG CACCCTCCTG 
GGAGCCTCGA TGGACATCCA CGGAGGGGGC TTCGACCTCC GCTTCCCTCA CCACGACAAC 
GAGCTGGCGC AGTCAGAGGC CTACTTTGAG AACGACTGCT GGGTCAGGTA CTTCCTGCAC 
ACAGGCCACC TGACCATCGC GGGCTGCAAG ATGTCCAAGT CGCTCAAGAA CTTCATCACC 
ATTAAAGACG CCCTGAAGAA GCACTCAGCC CGCCAGCTGC GCCTGGCCTT CCTCATGCAC 
TCGTGGAAGG ACACGCTGGA CTACTCGGCC AACACCATGG AGTCGGCGCT GCAGTACGAG 
AGGCTCCTCA ATGAGTTTTT CTTAAACGTG AAAGACCTCC TCCGAGCCCC CGTGGAGGTG 
GCCGCTCAGT TTGAGAAGTG GGAGGACGAG GAAACGGAGC TGAACAAGAG CTTTTACAAG 
AAGAAGGCCG CGGTTCACCA GGCGCTCTGT GACAACATCG ACACGCGCAC AGTCCTGGAG 
GAGATGCGGG CTCTGGTCGG CCAGTGCAAC CTCTACATGG CGGCCCGCAA GGCCGCCCGG 
AGGAGGCCCC ACCGCGTCCT GCTGGAGAGC ATCGCCCACT ACCTCACACA CATGCTGAAG 
ATCTTCGGGG CCATAGAAGA GGAAAGCTCC TTGGGTTTCC CCATCGGAGG AGCTGGAACC 
AGCCTCAATC TGGAGTCCAC GGTCATGCCC TACCTGCAGG TGCTGTCGGA GTTCCGGGAA 
GGGGTGCGGC GGATCGCCCG TGAGCACAGA GTGCCTGAGG TCCTCCAGCT GAGCGACGCC 
CTGCGGGACA ACGTCCTGCC CGAGCTGGGG GTGCGGCTGG AAGACCACGA AGGTCTGCCC 
ACCGTGGTCA AGCTGGTGGA CAGAGACGCC TTACTGAGAG AGAGAGAAGA CAAGAAAAGG 
GCGGAAGAGG AGAAAAGGAA GAAGAAAGAG GAGGCTACCA GGAAGAGACA GGAGCAGGAG 
GCAGCAAAGC TGGCCAAGAT GAAGATCCCA CCCAGTGAGA TGTTCTTATC GGAAAGCGAC 
AAATACTCCA AGTTCGACGA AAATGGTCTG CCCACGCACG ACACCGAAGG CAAAGAGCTG 
AGCAAGGGGC AGGCCAAGAA GCTGCGGAAG CTGTTCGAGG CGCAGGAGAA GCTGCACTTA 
GAGTACCTGC AACTGGTTCA GAACGGGGGC CCCCCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001096719.1
CDS5..2503
Translation

Target ORF information:

RefSeq Version NM_001103249.1
Organism Bos taurus(cattle)
Definition Bos taurus cysteinyl-tRNA synthetase 1 (CARS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001103249.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC 
TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC 
CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC 
CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAACAGGCG GAGGGCGGCG TGCACAGCCC 
ACATGGTCTC CTCCAGCTGG GTCTGAGCCA TGCCAGCTGC GCCTGTACAA CAGCCTGACA 
CGGAGGAAGG ACGTGTTTGT GCCGCAAGAC GGAAGGAGGG TGACATGGTA CTGCTGCGGG 
CCCACCGTCT ACGACGCCTC GCACATGGGG CACGCCAGGT CCTACATCTC CTTCGATATC 
TTGAGAAGAG TGCTGAGGGA TTACTTCAAA TTTGACGTCT TCTACTGCAT GAACATCACG 
GACATTGACG ATAAGATCAT CAAGAGGGCC CGGCAGAACT TCCTGTTGGA GCGGTACCGG 
GAGACGCAGC CCCAGGCAGC GCAGCTGCTG GAGGACGTGC GAGCCGCCCT GCAGCCGTTC 
TCCGCGAAGC TCCAGGAGAC TGTGGACCCG GACAAGAAGC AGATGCTGGA ACGCCTGCAG 
CACGCGGTGG CGCAGGCCGC GGAGCCGCTG GAGGGCGCGC TGCGGGCCGG GCAGGCCGGG 
CAGGAGCTGG ACGGCCGCGT GCAGGCGTTG CTGGAAGAAG CAAAGGATGT GCTTTCTGAC 
TGGCTGGATT CCATGCACGG CAGTGAGGTG ACGGACAATT CTATTTTCTC TGAACTGCCC 
AAATTCTGGG AGGCGGAGTT CCACAAAGAC ATGGAAGCCC TGAACGTCCT CCCTCCGGAC 
GTCCTGACCC GGGTTAGTGA GTATGTGCCA GAGATCGTGG ACTTTGTCCA GAAGATCGTG 
GACAACGGTT ACGGGTATGC TTCAAACGGG TCTGTGTACT TCGACACAGT CAAGTTTGCC 
AACACTGAGG GGCACAGCTA CGGAAAGCTG GTGCCCGAGG CCGTCGGGGA CCAGAAAGCC 
CTGCACGAGG GGGAAGGCGA CCTGAGCGTC TCTGCAGAGC GCCTGAGTGA GAAGCGGTCC 
CCCAATGACT TTGCGCTCTG GAAGGCCTCC AAGCCCGGGG AGCCGTCCTG GCCCTGCCCC 
TGGGGCAAGG GGCGCCCCGG ATGGCACATC GAGTGCTCGG CCATGGCGGG CACCCTCCTG 
GGAGCCTCGA TGGACATCCA CGGAGGGGGC TTCGACCTCC GCTTCCCTCA CCACGACAAC 
GAGCTGGCGC AGTCAGAGGC CTACTTTGAG AACGACTGCT GGGTCAGGTA CTTCCTGCAC 
ACAGGCCACC TGACCATCGC GGGCTGCAAG ATGTCCAAGT CGCTCAAGAA CTTCATCACC 
ATTAAAGACG CCCTGAAGAA GCACTCAGCC CGCCAGCTGC GCCTGGCCTT CCTCATGCAC 
TCGTGGAAGG ACACGCTGGA CTACTCGGCC AACACCATGG AGTCGGCGCT GCAGTACGAG 
AGGCTCCTCA ATGAGTTTTT CTTAAACGTG AAAGACCTCC TCCGAGCCCC CGTGGAGGTG 
GCCGCTCAGT TTGAGAAGTG GGAGGACGAG GAAACGGAGC TGAACAAGAG CTTTTACAAG 
AAGAAGGCCG CGGTTCACCA GGCGCTCTGT GACAACATCG ACACGCGCAC AGTCCTGGAG 
GAGATGCGGG CTCTGGTCGG CCAGTGCAAC CTCTACATGG CGGCCCGCAA GGCCGCCCGG 
AGGAGGCCCC ACCGCGTCCT GCTGGAGAGC ATCGCCCACT ACCTCACACA CATGCTGAAG 
ATCTTCGGGG CCATAGAAGA GGAAAGCTCC TTGGGTTTCC CCATCGGAGG AGCTGGAACC 
AGCCTCAATC TGGAGTCCAC GGTCATGCCC TACCTGCAGG TGCTGTCGGA GTTCCGGGAA 
GGGGTGCGGC GGATCGCCCG TGAGCACAGA GTGCCTGAGG TCCTCCAGCT GAGCGACGCC 
CTGCGGGACA ACGTCCTGCC CGAGCTGGGG GTGCGGCTGG AAGACCACGA AGGTCTGCCC 
ACCGTGGTCA AGCTGGTGGA CAGAGACGCC TTACTGAGAG AGAGAGAAGA CAAGAAAAGG 
GCGGAAGAGG AGAAAAGGAA GAAGAAAGAG GAGGCTACCA GGAAGAGACA GGAGCAGGAG 
GCAGCAAAGC TGGCCAAGAT GAAGATCCCA CCCAGTGAGA TGTTCTTATC GGAAAGCGAC 
AAATACTCCA AGTTCGACGA AAATGGTCTG CCCACGCACG ACACCGAAGG CAAAGAGCTG 
AGCAAGGGGC AGGCCAAGAA GCTGCGGAAG CTGTTCGAGG CGCAGGAGAA GCTGCACTTA 
GAGTACCTGC AACTGGTTCA GAACGGGGGC CCCCCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa89150
Clone ID Related Accession (Same CDS sequence) XM_015461344.1
Accession Version XM_015461344.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product cysteine--tRNA ligase, cytoplasmic isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC 
TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC 
CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC 
CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAAGCGGAG GGCGGCGTGC ACAGCCCACA 
TGGTCTCCTC CAGCTGGGTC TGAGCCATGC CAGCTGCGCC TGTACAACAG CCTGACACGG 
AGGAAGGACG TGTTTGTGCC GCAAGACGGA AGGAGGGTGA CATGGTACTG CTGCGGGCCC 
ACCGTCTACG ACGCCTCGCA CATGGGGCAC GCCAGGTCCT ACATCTCCTT CGATATCTTG 
AGAAGAGTGC TGAGGGATTA CTTCAAATTT GACGTCTTCT ACTGCATGAA CATCACGGAC 
ATTGACGATA AGATCATCAA GAGGGCCCGG CAGAACTTCC TGTTGGAGCG GTACCGGGAG 
ACGCAGCCCC AGGCAGCGCA GCTGCTGGAG GACGTGCGAG CCGCCCTGCA GCCGTTCTCC 
GCGAAGCTCC AGGAGACTGT GGACCCGGAC AAGAAGCAGA TGCTGGAACG CCTGCAGCAC 
GCGGTGGCGC AGGCCGCGGA GCCGCTGGAG GGCGCGCTGC GGGCCGGGCA GGCCGGGCAG 
GAGCTGGACG GCCGCGTGCA GGCGTTGCTG GAAGAAGCAA AGGATGTGCT TTCTGACTGG 
CTGGATTCCA TGCACGGCAG TGAGGTGACG GACAATTCTA TTTTCTCTGA ACTGCCCAAA 
TTCTGGGAGG CGGAGTTCCA CAAAGACATG GAAGCCCTGA ACGTCCTCCC TCCGGACGTC 
CTGACCCGGG TTAGTGAGTA TGTGCCAGAG ATCGTGGACT TTGTCCAGAA GATCGTGGAC 
AACGGTTACG GGTATGCTTC AAACGGGTCT GTGTACTTCG ACACAGTCAA GTTTGCCAAC 
ACTGAGGGGC ACAGCTACGG AAAGCTGGTG CCCGAGGCCG TCGGGGACCA GAAAGCCCTG 
CACGAGGGGG AAGGCGACCT GAGCGTCTCT GCAGAGCGCC TGAGTGAGAA GCGGTCCCCC 
AATGACTTTG CGCTCTGGAA GGCCTCCAAG CCCGGGGAGC CGTCCTGGCC CTGCCCCTGG 
GGCAAGGGGC GCCCCGGATG GCACATCGAG TGCTCGGCCA TGGCGGGCAC CCTCCTGGGA 
GCCTCGATGG ACATCCACGG AGGGGGCTTC GACCTCCGCT TCCCTCACCA CGACAACGAG 
CTGGCGCAGT CAGAGGCCTA CTTTGAGAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATCGCGGG CTGCAAGATG TCCAAGTCGC TCAAGAACTT CATCACCATT 
AAAGACGCCC TGAAGAAGCA CTCAGCCCGC CAGCTGCGCC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CGCTGGACTA CTCGGCCAAC ACCATGGAGT CGGCGCTGCA GTACGAGAGG 
CTCCTCAATG AGTTTTTCTT AAACGTGAAA GACCTCCTCC GAGCCCCCGT GGAGGTGGCC 
GCTCAGTTTG AGAAGTGGGA GGACGAGGAA ACGGAGCTGA ACAAGAGCTT TTACAAGAAG 
AAGGCCGCGG TTCACCAGGC GCTCTGTGAC AACATCGACA CGCGCACAGT CCTGGAGGAG 
ATGCGGGCTC TGGTCGGCCA GTGCAACCTC TACATGGCGG CCCGCAAGGC CGCCCGGAGG 
AGGCCCCACC GCGTCCTGCT GGAGAGCATC GCCCACTACC TCACACACAT GCTGAAGATC 
TTCGGGGCCA TAGAAGAGGA AAGCTCCTTG GGTTTCCCCA TCGGAGGAGC TGGAACCAGC 
CTCAATCTGG AGTCCACGGT CATGCCCTAC CTGCAGGTGC TGTCGGAGTT CCGGGAAGGG 
GTGCGGCGGA TCGCCCGTGA GCACAGAGTG CCTGAGGTCC TCCAGCTGAG CGACGCCCTG 
CGGGACAACG TCCTGCCCGA GCTGGGGGTG CGGCTGGAAG ACCACGAAGG TCTGCCCACC 
GTGGTCAAGC TGGTGGACAG AGACGCCTTA CTGAGAGAGA GAGAAGACAA GAAAAGGGCG 
GAAGAGGAGA AAAGGAAGAA GAAAGAGGAG GCTACCAGGA AGAGACAGGA GCAGGAGGCA 
GCAAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTATCGGA AAGCGACAAA 
TACTCCAAGT TCGACGAAAA TGGTCTGCCC ACGCACGACA CCGAAGGCAA AGAGCTGAGC 
AAGGGGCAGG CCAAGAAGCT GCGGAAGCTG TTCGAGGCGC AGGAGAAGCT GCACTTAGAG 
TACCTGCAAC TGGTTCAGAA CGGGGGCCCC CCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015316830.1
CDS83..2578
Translation

Target ORF information:

RefSeq Version XM_015461344.1
Organism Bos taurus(cattle)
Definition Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015461344.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC 
TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC 
CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC 
CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAAGCGGAG GGCGGCGTGC ACAGCCCACA 
TGGTCTCCTC CAGCTGGGTC TGAGCCATGC CAGCTGCGCC TGTACAACAG CCTGACACGG 
AGGAAGGACG TGTTTGTGCC GCAAGACGGA AGGAGGGTGA CATGGTACTG CTGCGGGCCC 
ACCGTCTACG ACGCCTCGCA CATGGGGCAC GCCAGGTCCT ACATCTCCTT CGATATCTTG 
AGAAGAGTGC TGAGGGATTA CTTCAAATTT GACGTCTTCT ACTGCATGAA CATCACGGAC 
ATTGACGATA AGATCATCAA GAGGGCCCGG CAGAACTTCC TGTTGGAGCG GTACCGGGAG 
ACGCAGCCCC AGGCAGCGCA GCTGCTGGAG GACGTGCGAG CCGCCCTGCA GCCGTTCTCC 
GCGAAGCTCC AGGAGACTGT GGACCCGGAC AAGAAGCAGA TGCTGGAACG CCTGCAGCAC 
GCGGTGGCGC AGGCCGCGGA GCCGCTGGAG GGCGCGCTGC GGGCCGGGCA GGCCGGGCAG 
GAGCTGGACG GCCGCGTGCA GGCGTTGCTG GAAGAAGCAA AGGATGTGCT TTCTGACTGG 
CTGGATTCCA TGCACGGCAG TGAGGTGACG GACAATTCTA TTTTCTCTGA ACTGCCCAAA 
TTCTGGGAGG CGGAGTTCCA CAAAGACATG GAAGCCCTGA ACGTCCTCCC TCCGGACGTC 
CTGACCCGGG TTAGTGAGTA TGTGCCAGAG ATCGTGGACT TTGTCCAGAA GATCGTGGAC 
AACGGTTACG GGTATGCTTC AAACGGGTCT GTGTACTTCG ACACAGTCAA GTTTGCCAAC 
ACTGAGGGGC ACAGCTACGG AAAGCTGGTG CCCGAGGCCG TCGGGGACCA GAAAGCCCTG 
CACGAGGGGG AAGGCGACCT GAGCGTCTCT GCAGAGCGCC TGAGTGAGAA GCGGTCCCCC 
AATGACTTTG CGCTCTGGAA GGCCTCCAAG CCCGGGGAGC CGTCCTGGCC CTGCCCCTGG 
GGCAAGGGGC GCCCCGGATG GCACATCGAG TGCTCGGCCA TGGCGGGCAC CCTCCTGGGA 
GCCTCGATGG ACATCCACGG AGGGGGCTTC GACCTCCGCT TCCCTCACCA CGACAACGAG 
CTGGCGCAGT CAGAGGCCTA CTTTGAGAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATCGCGGG CTGCAAGATG TCCAAGTCGC TCAAGAACTT CATCACCATT 
AAAGACGCCC TGAAGAAGCA CTCAGCCCGC CAGCTGCGCC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CGCTGGACTA CTCGGCCAAC ACCATGGAGT CGGCGCTGCA GTACGAGAGG 
CTCCTCAATG AGTTTTTCTT AAACGTGAAA GACCTCCTCC GAGCCCCCGT GGAGGTGGCC 
GCTCAGTTTG AGAAGTGGGA GGACGAGGAA ACGGAGCTGA ACAAGAGCTT TTACAAGAAG 
AAGGCCGCGG TTCACCAGGC GCTCTGTGAC AACATCGACA CGCGCACAGT CCTGGAGGAG 
ATGCGGGCTC TGGTCGGCCA GTGCAACCTC TACATGGCGG CCCGCAAGGC CGCCCGGAGG 
AGGCCCCACC GCGTCCTGCT GGAGAGCATC GCCCACTACC TCACACACAT GCTGAAGATC 
TTCGGGGCCA TAGAAGAGGA AAGCTCCTTG GGTTTCCCCA TCGGAGGAGC TGGAACCAGC 
CTCAATCTGG AGTCCACGGT CATGCCCTAC CTGCAGGTGC TGTCGGAGTT CCGGGAAGGG 
GTGCGGCGGA TCGCCCGTGA GCACAGAGTG CCTGAGGTCC TCCAGCTGAG CGACGCCCTG 
CGGGACAACG TCCTGCCCGA GCTGGGGGTG CGGCTGGAAG ACCACGAAGG TCTGCCCACC 
GTGGTCAAGC TGGTGGACAG AGACGCCTTA CTGAGAGAGA GAGAAGACAA GAAAAGGGCG 
GAAGAGGAGA AAAGGAAGAA GAAAGAGGAG GCTACCAGGA AGAGACAGGA GCAGGAGGCA 
GCAAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTATCGGA AAGCGACAAA 
TACTCCAAGT TCGACGAAAA TGGTCTGCCC ACGCACGACA CCGAAGGCAA AGAGCTGAGC 
AAGGGGCAGG CCAAGAAGCT GCGGAAGCTG TTCGAGGCGC AGGAGAAGCT GCACTTAGAG 
TACCTGCAAC TGGTTCAGAA CGGGGGCCCC CCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL