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PUS3 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol PUS3
Entrez Gene ID 515824
Full Name pseudouridylate synthase 3
General protein information
Preferred Names

pseudouridylate synthase 3

Names

tRNA pseudouridine(38/39) synthase
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_010820931.2 XP_010819233.1 tRNA pseudouridine(38/39) synthase isoform X1
XM_010820931.3 XP_010819233.1 tRNA pseudouridine(38/39) synthase isoform X1
NM_001034512.1 NP_001029684.1 tRNA pseudouridine(38/39) synthase
NM_001034512.2 NP_001029684.1 tRNA pseudouridine(38/39) synthase

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) PUS3 XP_536533.1
Caenorhabditis elegans (roundworm) tag-124 NP_496062.3
Schizosaccharomyces pombe (fission yeast) SPAC25B8.05 NP_594465.1
Xenopus tropicalis (tropical clawed frog) pus3 NP_988969.1
Pan troglodytes (chimpanzee) PUS3 XP_001148378.1
PUS3 XP_001111887.1
Bos taurus (cattle) PUS3 NP_001029684.1
Gallus gallus (chicken) PUS3 XP_004948004.1
Danio rerio (zebrafish) pus3 NP_956361.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP010807 XP_318500.4
Mus musculus (house mouse) Pus3 NP_075781.3
Kluyveromyces lactis KLLA0E14345g XP_454596.1
Homo sapiens (human) PUS3 NP_112597.3
Rattus norvegicus (Norway rat) Pus3 NP_001101604.1
Drosophila melanogaster (fruit fly) CG3045 NP_611646.1
Saccharomyces cerevisiae (baker's yeast) DEG1 NP_116655.1
Eremothecium gossypii AGOS_AGL003W NP_986663.2

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PUS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa42808 NM_001034512.2
Latest version!		 Bos taurus pseudouridine synthase 3 (PUS3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
OBa34577 XM_010820931.3
Latest version!		 Bos taurus pseudouridylate synthase 3 (PUS3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
View Old Accession Versions >>
OBa34577 XM_010820931.2 Bos taurus pseudouridylate synthase 3 (PUS3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
OBa42808 NM_001034512.1 Bos taurus pseudouridylate synthase 3 (PUS3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa42808
Clone ID Related Accession (Same CDS sequence) NM_001034512.1 , NM_001034512.2
Accession Version NM_001034512.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-21
Organism Bos taurus(cattle)
Product tRNA pseudouridine(38/39) synthase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104569.1. On or before Oct 17, 2005 this sequence version replaced XM_876200.1, XM_593911.2, XM_876328.1. ##Evidence-Data-START## Transcript exon combination :: BC104569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00120237, SAMN00120238 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AATGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001029684.1
CDS51..1496
Translation

Target ORF information:

RefSeq Version NM_001034512.1
Organism Bos taurus(cattle)
Definition Bos taurus pseudouridylate synthase 3 (PUS3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001034512.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AATGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa42808
Clone ID Related Accession (Same CDS sequence) NM_001034512.1 , NM_001034512.2
Accession Version NM_001034512.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Bos taurus(cattle)
Product tRNA pseudouridine(38/39) synthase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104569.1. On Sep 15, 2018 this sequence version replaced NM_001034512.1. ##Evidence-Data-START## Transcript exon combination :: BC104569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145444, SAMN03145467 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AATGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001029684.1
CDS46..1491
Translation

Target ORF information:

RefSeq Version NM_001034512.2
Organism Bos taurus(cattle)
Definition Bos taurus pseudouridine synthase 3 (PUS3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001034512.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AATGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa34577
Clone ID Related Accession (Same CDS sequence) XM_010820931.2 , XM_010820931.3
Accession Version XM_010820931.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product tRNA pseudouridine(38/39) synthase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104641.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AACGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010819233.1
CDS351..1796
Translation

Target ORF information:

RefSeq Version XM_010820931.2
Organism Bos taurus(cattle)
Definition Bos taurus pseudouridylate synthase 3 (PUS3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010820931.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AACGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa34577
Clone ID Related Accession (Same CDS sequence) XM_010820931.2 , XM_010820931.3
Accession Version XM_010820931.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product tRNA pseudouridine(38/39) synthase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AACGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010819233.1
CDS185..1630
Translation

Target ORF information:

RefSeq Version XM_010820931.3
Organism Bos taurus(cattle)
Definition Bos taurus pseudouridylate synthase 3 (PUS3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010820931.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCTGAAA ATGACGTAGA CAGAATCCAG ACTGAGAAAC TCCTAAAAAG AGTACAGGAA 
CTGGAGCAGG AGGTAAAACG ACTTAAAAAA GAACAGGCCA ACAATAAGGA CTCAAACATT 
AGAGAGAATT CTTCAGGAGC TGGGGGAAAA CCTAAGCGTG CCTTTGATTT CAGTGCTCAC 
GGTCAAAGAC ATGTAGCTCT AAAGATCGCC TACCTGGGCT GGGGATATCA GGGCTTTGCC 
AGTCAGGAAA ACACAAGCAA TACAATTGAA GAGAAACTGT TTGAGGCTCT AACCAAGACT 
CGACTGGTAG AAAACAGGCA GACATCCAAC TATCACCGGT GTGGGCGAAC AGACAAAGGA 
GTCAGTGCCT TTGGACAGGT GATTTCTCTT GACCTACGTT CTCACATTCC AAAGGGCAGG 
GATTCTGAGC ATTTTAATTT AAAAAACGAA GTCAATGATG TGGCTACAGA GATCCGTTAT 
ACCCACATTC TCAATCGGGT ACTCCCTCCA GACATCCGTG TGCTGGCCTG GGCTCCCGTA 
GAAACTAGCT TCAGTGCTAG GTTCAGCTGT CTTGAGCGGA CCTACCGCTA TTTTTTCCCT 
CGTGCTAACT TAGACATTGT AACCATGAAC TATGCAGCTC AGAAGTATGT TGGCACACAT 
GATTTTAGGA ACTTATGTAA AATGGATGTA GCCAATGGGG TGATTAATTT TCAGAGGACT 
ATCTTGTCTG CTCAAGTACA GCGAGTGGGC CAGAACCTGG GAGAGGAGGG ATGGCAAGAA 
CCCTTTCAGT TATGCCAGTT TGAAGTGACT GGCCAGGCGT TCCTTTATCA TCAAGTCCGC 
TGTATGATGG CTGTTCTTTT TCTGATTGGC CAAGGAATGG AGAAGCCAGA GGTTATTGAT 
GAACTGTTGA ACATAGAGAA AAATCCCCAG AAGCCTCAAT ATAGTATGGC TGTGGAATTT 
CCTCTAGTCT TATATGACTG TAAGTTTGAA AATATTAAGT GGATCTATGA CCGGGAAGTT 
CAGGAATTCA ATGTTACCCA CCTTCAGCAA CTATGGGCTA ATCATGCTGT GAAAACTCAG 
ATGTTGTATA GTATGCTACA AGGCCTGGAC TCTGTTGCAC TACCCTGTGG AACAGGACCA 
AAGATGGATG GAATGATAGA ATGGAGAAAT GTTAAGCCCT CTGTCACAAA GCAGACCAGT 
GCCTTTGTAG AAGGAGTGAA AATGCGCACT TATAAGCCCC TAATGGATCG TCCTAAATGC 
CAAGGATTAG AATCCCGGAT CCAGCATTTT GTACGTAGGG GACGCATTGA ACACCCACAT 
TTATTCCATG AGGAAGAAAC AAAAGCCAAA AGGGACTGTA GTGACACACT AGAGGAAGAA 
AATACTGTTT TTGAGAAACC AACGAAGAGA ATTTGTGTTG ATACAGAACT TAAAAGCATC 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL