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PTPN22 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol PTPN22
Entrez Gene ID 518992
Full Name protein tyrosine phosphatase, non-receptor type 22
General protein information
Preferred Names

protein tyrosine phosphatase, non-receptor type 22

Names

tyrosine-protein phosphatase non-receptor type 22
protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_024988885.1 XP_024844653.1 tyrosine-protein phosphatase non-receptor type 22 isoform X2
NM_001192503.2 NP_001179432.2 tyrosine-protein phosphatase non-receptor type 22
XM_024988884.1 XP_024844652.1 tyrosine-protein phosphatase non-receptor type 22 isoform X1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) PTPN22 XP_513663.2
Bos taurus (cattle) PTPN22 NP_001179432.2
Mus musculus (house mouse) Ptpn22 NP_033005.1
Gallus gallus (chicken) PTPN22 XP_001235234.1
PTPN22 XP_001110241.1
Rattus norvegicus (Norway rat) Ptpn22 NP_001099930.1
Xenopus tropicalis (tropical clawed frog) ptpn22 XP_002932177.2
Homo sapiens (human) PTPN22 NP_057051.3
Canis lupus familiaris (dog) PTPN22 XP_540240.3

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTPN22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTPN22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01656 NM_001192503.2
Latest version!		 Bos taurus protein tyrosine phosphatase non-receptor type 22 (PTPN22), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OBa232757 XM_024988884.1
Latest version!		 Bos taurus protein tyrosine phosphatase, non-receptor type 22 (PTPN22), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OBa232758 XM_024988885.1
Latest version!		 Bos taurus protein tyrosine phosphatase, non-receptor type 22 (PTPN22), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa01656
Clone ID Related Accession (Same CDS sequence) NM_001192503.2
Accession Version NM_001192503.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-12
Organism Bos taurus(cattle)
Product tyrosine-protein phosphatase non-receptor type 22
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000003.1 and DT841911.1. On Apr 19, 2011 this sequence version replaced NM_001192503.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145426, SAMN03145442 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGACCAAA GAGAAATTCT GCAGAAGTTC TTGGATGAGG CACAACACAG GAAGAGTAAC 
AAAGAGGAGT TTGCTAATGA ATTTCTGACG CTGAAAAGAT TATCCTCCAA GTATAAGGCA 
GATAAAACCT ATCCTACAAC TGTGGCTGAG AGGCCCAAGA ATATCAAGAA AAACAGATAT 
AAGGATATTT TGCCCTATGA CCATAGCCTA GTAGAACTGT GCATGATAAC GTCTGATGAG 
GATTCCAGCT ACATCAATGC CAACTTCATT AAGGGAGTTT ATGGACCCAA GACTTATATT 
GCCACCCAGG GACCTTTGCC TACAACTGTC CTGGACTTCT GGAGGATGAT TTGGGAATAC 
AGCATCCTGA TCATTGTTAT GGCGTGCATG GAGTTTGAAA TGGGAAAGAA AAAATGTGAG 
CGCTACTGGG CTGAACCAGG AGACACGCAA CTGCAACTTG GTCCTTTCTC CATATCCTGT 
GAAGCTGAAA ATAGAAAATC TGATTATACA ATCAGGACTC TAAAAGCCAA GTTCAATAGT 
GAAACTCGAA CCATCTATCA GTTCCATTAT AAGAACTGGC CAGACCATGA TGTGCCTTCA 
TCTATAGACC CTATTCTTGA GCTCATTTGG GATGTGCGTT GTTACCAAGA CGATAACAGT 
GTTCCCATAT GCATTCACTG CAGTGCTGGT TGTGGAAGGA CTGGTGTTCT CTGTGCTATT 
GATTACACGT GGATGTTGCT AAAAGATGGG ATAATTCCTG AGAACTTCAG TGTTTTTAGT 
TTGATCCAGG AAATGCGAAC ACAGAGGCCT TCATTAGTTC AAACTCAGCA ACAGTATGAA 
CTGGTCTACA ACGCTGTATT AGAACTATTT ACGAGACAGA TGGATATTAT CGGGGATAAA 
CACTCTGGAA CAGAGATTCA AGCAAAGGAT TCAGTTTTTG AGCAAAATCC CAACATAGAA 
GCAAAATCTT ATTCTCCTAG TTTACCAAAA AGTAACATAA AAGAAGCAAC AGTGATGAAC 
CAACAGAGCA AAGGAAGGAT AAGGGTGAAA AGTGCAGAAG CTTCTTCCTT TGACCTCAGG 
ACTTCCGAAA TAAATGAAAA GGAAGGGTTA GTCATGCACT CTGCTAAACA GAGCAGTTCT 
TTAGAGTTTT TGGAACTGAA CTGTGGTTGT AACAAAAATG CTGACACAAC CATGAAATGG 
GAGACAAAGG TATTCCCAAT AGCTGGGGAG CCTCTTCAGA AGCATCAAAG TTTGGACTTG 
AGTTCCATTT TGTTTGGAGC ATATCCTAAA TCTAAACCTG TAAATGCAGC AGGAAGATAT 
TCTAATTTAA AGAAGCCAAT ACTGCGGACC AAATCAACTC CCTTTGAATT GATGCAAGAG 
AGAGACAGCA AAGAGTTGGA CATAAAGGGA AATTTTTATT TGGAATCTCA ACCACACAAT 
TCTTGTGTTG ATCAGGCTCA AAAAGTGATG CATGTTTCTA CAGCAGAACT GAATTTTTCA 
CCATTACATG ACTCTAAGCA TCAAACATGC AATGCTGCTA ATGCAAAGCA GCATGACTCT 
GGTGCTTTAA ATGAGCATTC TTATATGTCT TTAGTAGAAG ATCCTTATTT TTCATCATTG 
TCTCCAAGTA GTGCTCATTC TAAGACGTCT TTTAATTTAC CTCAGAAGCA AGATGGAAGT 
GTTTTACCTT GTTCTTCACT GTCAAAATCC TCTACAACCC TCTTTCCTTA TTACAATTCA 
CATGATTCTT TAACACTGAG TACTCTGACC AACTTTCCCC CACCACTGAA CCAAGAGACA 
GCAGCAGTAG CAACTTCTCC AAGGATAGAT GATGAAATCC CTCCTCCACT TCCTGAACGG 
ACACCTGAAT CTTTTATTGT AGTAGAGGAA GCCGGAGAAC TCCCACAGGG TGTTCCCACA 
TCTTCATCTT CACCTGTGAA GGTAAAAATT GGAACATCAC TAGAATGCAG TGGAACATCT 
GAATCAAAGA AATCTGATGA TTTGGTAAGA CTTAGACCAA GTAATAGTGT AAAACCCCGG 
AGTCCAAAAT CAGATCTACA ACAAGATCGT TCTTCTTCCC CACCACCTCC ACTGCCAGAG 
AGAACCTTAG AGTCCTTCCT TCTTGCTGAT GAAGATTGTA CACAGGCTCA ACCTGTAGAG 
ACTTGTTCTA CGAGCTGTCC TAACACCATG GAAAATTCAA CATCTTCAAA ACAGACACTG 
AAACCTCCTG GAAAGAATTT TACAAGGAGT AAGAGTTTGA AAATTTTACG AAACGTGAAA 
AAGAGTATCT GTAATTCTTC CCCACCAAAC AAGCCTGCAG AGACTGCTCG GTCAAAAAAC 
TCCAGCTCCT TTTTGAATTT TGGTTTTGCA AATCGTTTTT CAAAACCCAA AGGACCAAGG 
AACCCACCAT CAACTTGGAA AATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001179432.2
CDS1..2427
Translation

Target ORF information:

RefSeq Version NM_001192503.2
Organism Bos taurus(cattle)
Definition Bos taurus protein tyrosine phosphatase non-receptor type 22 (PTPN22), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001192503.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGACCAAA GAGAAATTCT GCAGAAGTTC TTGGATGAGG CACAACACAG GAAGAGTAAC 
AAAGAGGAGT TTGCTAATGA ATTTCTGACG CTGAAAAGAT TATCCTCCAA GTATAAGGCA 
GATAAAACCT ATCCTACAAC TGTGGCTGAG AGGCCCAAGA ATATCAAGAA AAACAGATAT 
AAGGATATTT TGCCCTATGA CCATAGCCTA GTAGAACTGT GCATGATAAC GTCTGATGAG 
GATTCCAGCT ACATCAATGC CAACTTCATT AAGGGAGTTT ATGGACCCAA GACTTATATT 
GCCACCCAGG GACCTTTGCC TACAACTGTC CTGGACTTCT GGAGGATGAT TTGGGAATAC 
AGCATCCTGA TCATTGTTAT GGCGTGCATG GAGTTTGAAA TGGGAAAGAA AAAATGTGAG 
CGCTACTGGG CTGAACCAGG AGACACGCAA CTGCAACTTG GTCCTTTCTC CATATCCTGT 
GAAGCTGAAA ATAGAAAATC TGATTATACA ATCAGGACTC TAAAAGCCAA GTTCAATAGT 
GAAACTCGAA CCATCTATCA GTTCCATTAT AAGAACTGGC CAGACCATGA TGTGCCTTCA 
TCTATAGACC CTATTCTTGA GCTCATTTGG GATGTGCGTT GTTACCAAGA CGATAACAGT 
GTTCCCATAT GCATTCACTG CAGTGCTGGT TGTGGAAGGA CTGGTGTTCT CTGTGCTATT 
GATTACACGT GGATGTTGCT AAAAGATGGG ATAATTCCTG AGAACTTCAG TGTTTTTAGT 
TTGATCCAGG AAATGCGAAC ACAGAGGCCT TCATTAGTTC AAACTCAGCA ACAGTATGAA 
CTGGTCTACA ACGCTGTATT AGAACTATTT ACGAGACAGA TGGATATTAT CGGGGATAAA 
CACTCTGGAA CAGAGATTCA AGCAAAGGAT TCAGTTTTTG AGCAAAATCC CAACATAGAA 
GCAAAATCTT ATTCTCCTAG TTTACCAAAA AGTAACATAA AAGAAGCAAC AGTGATGAAC 
CAACAGAGCA AAGGAAGGAT AAGGGTGAAA AGTGCAGAAG CTTCTTCCTT TGACCTCAGG 
ACTTCCGAAA TAAATGAAAA GGAAGGGTTA GTCATGCACT CTGCTAAACA GAGCAGTTCT 
TTAGAGTTTT TGGAACTGAA CTGTGGTTGT AACAAAAATG CTGACACAAC CATGAAATGG 
GAGACAAAGG TATTCCCAAT AGCTGGGGAG CCTCTTCAGA AGCATCAAAG TTTGGACTTG 
AGTTCCATTT TGTTTGGAGC ATATCCTAAA TCTAAACCTG TAAATGCAGC AGGAAGATAT 
TCTAATTTAA AGAAGCCAAT ACTGCGGACC AAATCAACTC CCTTTGAATT GATGCAAGAG 
AGAGACAGCA AAGAGTTGGA CATAAAGGGA AATTTTTATT TGGAATCTCA ACCACACAAT 
TCTTGTGTTG ATCAGGCTCA AAAAGTGATG CATGTTTCTA CAGCAGAACT GAATTTTTCA 
CCATTACATG ACTCTAAGCA TCAAACATGC AATGCTGCTA ATGCAAAGCA GCATGACTCT 
GGTGCTTTAA ATGAGCATTC TTATATGTCT TTAGTAGAAG ATCCTTATTT TTCATCATTG 
TCTCCAAGTA GTGCTCATTC TAAGACGTCT TTTAATTTAC CTCAGAAGCA AGATGGAAGT 
GTTTTACCTT GTTCTTCACT GTCAAAATCC TCTACAACCC TCTTTCCTTA TTACAATTCA 
CATGATTCTT TAACACTGAG TACTCTGACC AACTTTCCCC CACCACTGAA CCAAGAGACA 
GCAGCAGTAG CAACTTCTCC AAGGATAGAT GATGAAATCC CTCCTCCACT TCCTGAACGG 
ACACCTGAAT CTTTTATTGT AGTAGAGGAA GCCGGAGAAC TCCCACAGGG TGTTCCCACA 
TCTTCATCTT CACCTGTGAA GGTAAAAATT GGAACATCAC TAGAATGCAG TGGAACATCT 
GAATCAAAGA AATCTGATGA TTTGGTAAGA CTTAGACCAA GTAATAGTGT AAAACCCCGG 
AGTCCAAAAT CAGATCTACA ACAAGATCGT TCTTCTTCCC CACCACCTCC ACTGCCAGAG 
AGAACCTTAG AGTCCTTCCT TCTTGCTGAT GAAGATTGTA CACAGGCTCA ACCTGTAGAG 
ACTTGTTCTA CGAGCTGTCC TAACACCATG GAAAATTCAA CATCTTCAAA ACAGACACTG 
AAACCTCCTG GAAAGAATTT TACAAGGAGT AAGAGTTTGA AAATTTTACG AAACGTGAAA 
AAGAGTATCT GTAATTCTTC CCCACCAAAC AAGCCTGCAG AGACTGCTCG GTCAAAAAAC 
TCCAGCTCCT TTTTGAATTT TGGTTTTGCA AATCGTTTTT CAAAACCCAA AGGACCAAGG 
AACCCACCAT CAACTTGGAA AATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa232757
Clone ID Related Accession (Same CDS sequence) XM_024988884.1
Accession Version XM_024988884.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2406bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product tyrosine-protein phosphatase non-receptor type 22 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGACCAAA GAGAAATTCT GCAGAAGTTC TTGGATGAGG CACAACACAG GAAGAGTAAC 
AAAGAGGAGT TTGCTAATGA ATTTCTGACG CTGAAAAGAT TATCCTCCAA GTATAAGGCA 
GATAAAACCT ATCCTACAAC TGTGGCTGAG AGGCCCAAGA ATATCAAGAA AAACAGATAT 
AAGGATATTT TGCCCTATGA CCATAGCCTA GTAGAACTGT GCATGATAAC GTCTGATGAG 
GATTCCAGCT ACATCAATGC CAACTTCATT AAGGGAGTTT ATGGACCCAA GACTTATATT 
GCCACCCAGG GACCTTTGCC TACAACTGTC CTGGACTTCT GGAGGATGAT TTGGGAATAC 
AGCATCCTGA TCATTGTTAT GGCGTGCATG GAGTTTGAAA TGGGAAAGAA AAAATGTGAG 
CGCTACTGGG CTGAACCAGG AGACACGCAA CTGCAACTTG GTCCTTTCTC CATATCCTGT 
GAAGCTGAAA ATAGAAAATC TGATTATACA ATCAGGACTC TAAAAGCCAA GTTCAATAGT 
GAAACTCGAA CCATCTATCA GTTCCATTAT AAGAACTGGC CAGACCATGA TGTGCCTTCA 
TCTATAGACC CTATTCTTGA GCTCATTTGG GATGTGCGTT GTTACCAAGA CGATAACAGT 
GTTCCCATAT GCATTCACTG CAGTGCTGGT TGTGGAAGGA CTGGTGTTCT CTGTGCTATT 
GATTACACGT GGATGTTGCT AAAAGATGGG ATAATTCCTG AGAACTTCAG TGTTTTTAGT 
TTGATCCAGG AAATGCGAAC ACAGAGGCCT TCATTAGTTC AAACTCAGCA ACAGTATGAA 
CTGGTCTACA ACGCTGTATT AGAACTATTT ACGAGACAGA TGGATATTAT CGGGGATAAA 
CACTCTGGAA CAGAGATTCA AGCAAAGGAT TCAGTTTTTG AGCAAAATCC CAACATAGAA 
GCAAAATCTT ATTCTCCTAG TTTACCAAAA AGTAACATAA AAGAAGCAAC AGTGATGAAC 
CAACAGAGCA AAGGAAGGAT AAGGGTGAAA AGTGCAGAAG CTTCTTCCTT TGACCTCAGG 
ACTTCCGAAA TAAATGAAAA GGAAGGGTTA GTCATGCACT CTGCTAAACA GAGCAGTTCT 
TTAGAGTTTT TGGAACTGAA CTGTGGTTGT AACAAAAATG CTGACACAAC CATGAAATGG 
GAGACAAAGG TATTCCCAAT AGCTGGGGAG CCTCTTCAGA AGCATCAAAG TTTGGACTTG 
AGTTCCATTT TGTTTGGAGC ATATCCTAAA TCTAAACCTG TAAATGCAGC AGGAAGATAT 
TCTAATTTAA AGAAGCCAAT ACTGCGGACC AAATCAACTC CCTTTGAATT GATGCAAGAG 
AGAGACAGCA AAGAGTTGGA CATAAAGGGA AATTTTTATT TGGAATCTCA ACCACACAAT 
TCTTGTGTTG ATCAGGCTCA AAAAGTGATG CATGTTTCTA CAGCAGAACT GAATTTTTCA 
CCATTACATG ACTCTAAGCA TCAAACATGC AATGCTGCTA ATGCAAAGCA GCATGACTCT 
GGTGCTTTAA ATGAGCATTC TTATATGTCT TTAGTAGAAG ATCCTTATTT TTCATCATTG 
TCTCCAAGTA GTGCTCATTC TAAGACGTCT TTTAATTTAC CTCAGAAGCA AGATGGAAGT 
GTTTTACCTT GTTCTTCACT GTCAAAATCC TCTACAACCC TCTTTCCTTA TTACAATTCA 
CATGATTCTT TAACACTGAG TACTCTGACC AACTTTCCCC CACCACTGAA CCAAGAGACA 
GCAGCAGTAG CAACTTCTCC AAGGATAGAT GATGAAATCC CTCCTCCACT TCCTGAACGG 
ACACCTGAAT CTTTTATTGT AGTAGAGGAA GCCGGAGAAC TCCCACAGGG TGTTCCCACA 
TCTTCATCTT CACCTGTGAA GGTAAAAATT GGAACATCAC TAGAATGCAG TGGAACATCT 
GAATCAAAGA AATCTGATGA TTTGAGTGTA AAACCCCGGA GTCCAAAATC AGATCTACAA 
CAAGATCGTT CTTCTTCCCC ACCACCTCCA CTGCCAGAGA GAACCTTAGA GTCCTTCCTT 
CTTGCTGATG AAGATTGTAC ACAGGCTCAA CCTGTAGAGA CTTGTTCTAC GAGCTGTCCT 
AACACCATGG AAAATTCAAC ATCTTCAAAA CAGACACTGA AACCTCCTGG AAAGAATTTT 
ACAAGGAGTA AGAGTTTGAA AATTTTACGA AACGTGAAAA AGAGTATCTG TAATTCTTCC 
CCACCAAACA AGCCTGCAGA GACTGCTCGG TCAAAAAACT CCAGCTCCTT TTTGAATTTT 
GGTTTTGCAA ATCGTTTTTC AAAACCCAAA GGACCAAGGA ACCCACCATC AACTTGGAAA 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024844652.1
CDS140..2545
Translation

Target ORF information:

RefSeq Version XM_024988884.1
Organism Bos taurus(cattle)
Definition Bos taurus protein tyrosine phosphatase, non-receptor type 22 (PTPN22), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024988884.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGACCAAA GAGAAATTCT GCAGAAGTTC TTGGATGAGG CACAACACAG GAAGAGTAAC 
AAAGAGGAGT TTGCTAATGA ATTTCTGACG CTGAAAAGAT TATCCTCCAA GTATAAGGCA 
GATAAAACCT ATCCTACAAC TGTGGCTGAG AGGCCCAAGA ATATCAAGAA AAACAGATAT 
AAGGATATTT TGCCCTATGA CCATAGCCTA GTAGAACTGT GCATGATAAC GTCTGATGAG 
GATTCCAGCT ACATCAATGC CAACTTCATT AAGGGAGTTT ATGGACCCAA GACTTATATT 
GCCACCCAGG GACCTTTGCC TACAACTGTC CTGGACTTCT GGAGGATGAT TTGGGAATAC 
AGCATCCTGA TCATTGTTAT GGCGTGCATG GAGTTTGAAA TGGGAAAGAA AAAATGTGAG 
CGCTACTGGG CTGAACCAGG AGACACGCAA CTGCAACTTG GTCCTTTCTC CATATCCTGT 
GAAGCTGAAA ATAGAAAATC TGATTATACA ATCAGGACTC TAAAAGCCAA GTTCAATAGT 
GAAACTCGAA CCATCTATCA GTTCCATTAT AAGAACTGGC CAGACCATGA TGTGCCTTCA 
TCTATAGACC CTATTCTTGA GCTCATTTGG GATGTGCGTT GTTACCAAGA CGATAACAGT 
GTTCCCATAT GCATTCACTG CAGTGCTGGT TGTGGAAGGA CTGGTGTTCT CTGTGCTATT 
GATTACACGT GGATGTTGCT AAAAGATGGG ATAATTCCTG AGAACTTCAG TGTTTTTAGT 
TTGATCCAGG AAATGCGAAC ACAGAGGCCT TCATTAGTTC AAACTCAGCA ACAGTATGAA 
CTGGTCTACA ACGCTGTATT AGAACTATTT ACGAGACAGA TGGATATTAT CGGGGATAAA 
CACTCTGGAA CAGAGATTCA AGCAAAGGAT TCAGTTTTTG AGCAAAATCC CAACATAGAA 
GCAAAATCTT ATTCTCCTAG TTTACCAAAA AGTAACATAA AAGAAGCAAC AGTGATGAAC 
CAACAGAGCA AAGGAAGGAT AAGGGTGAAA AGTGCAGAAG CTTCTTCCTT TGACCTCAGG 
ACTTCCGAAA TAAATGAAAA GGAAGGGTTA GTCATGCACT CTGCTAAACA GAGCAGTTCT 
TTAGAGTTTT TGGAACTGAA CTGTGGTTGT AACAAAAATG CTGACACAAC CATGAAATGG 
GAGACAAAGG TATTCCCAAT AGCTGGGGAG CCTCTTCAGA AGCATCAAAG TTTGGACTTG 
AGTTCCATTT TGTTTGGAGC ATATCCTAAA TCTAAACCTG TAAATGCAGC AGGAAGATAT 
TCTAATTTAA AGAAGCCAAT ACTGCGGACC AAATCAACTC CCTTTGAATT GATGCAAGAG 
AGAGACAGCA AAGAGTTGGA CATAAAGGGA AATTTTTATT TGGAATCTCA ACCACACAAT 
TCTTGTGTTG ATCAGGCTCA AAAAGTGATG CATGTTTCTA CAGCAGAACT GAATTTTTCA 
CCATTACATG ACTCTAAGCA TCAAACATGC AATGCTGCTA ATGCAAAGCA GCATGACTCT 
GGTGCTTTAA ATGAGCATTC TTATATGTCT TTAGTAGAAG ATCCTTATTT TTCATCATTG 
TCTCCAAGTA GTGCTCATTC TAAGACGTCT TTTAATTTAC CTCAGAAGCA AGATGGAAGT 
GTTTTACCTT GTTCTTCACT GTCAAAATCC TCTACAACCC TCTTTCCTTA TTACAATTCA 
CATGATTCTT TAACACTGAG TACTCTGACC AACTTTCCCC CACCACTGAA CCAAGAGACA 
GCAGCAGTAG CAACTTCTCC AAGGATAGAT GATGAAATCC CTCCTCCACT TCCTGAACGG 
ACACCTGAAT CTTTTATTGT AGTAGAGGAA GCCGGAGAAC TCCCACAGGG TGTTCCCACA 
TCTTCATCTT CACCTGTGAA GGTAAAAATT GGAACATCAC TAGAATGCAG TGGAACATCT 
GAATCAAAGA AATCTGATGA TTTGAGTGTA AAACCCCGGA GTCCAAAATC AGATCTACAA 
CAAGATCGTT CTTCTTCCCC ACCACCTCCA CTGCCAGAGA GAACCTTAGA GTCCTTCCTT 
CTTGCTGATG AAGATTGTAC ACAGGCTCAA CCTGTAGAGA CTTGTTCTAC GAGCTGTCCT 
AACACCATGG AAAATTCAAC ATCTTCAAAA CAGACACTGA AACCTCCTGG AAAGAATTTT 
ACAAGGAGTA AGAGTTTGAA AATTTTACGA AACGTGAAAA AGAGTATCTG TAATTCTTCC 
CCACCAAACA AGCCTGCAGA GACTGCTCGG TCAAAAAACT CCAGCTCCTT TTTGAATTTT 
GGTTTTGCAA ATCGTTTTTC AAAACCCAAA GGACCAAGGA ACCCACCATC AACTTGGAAA 
ATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa232758
Clone ID Related Accession (Same CDS sequence) XM_024988885.1
Accession Version XM_024988885.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product tyrosine-protein phosphatase non-receptor type 22 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGACCAAA GAGAAATTCT GCAGAAGTTC TTGGATGAGG CACAACACAG GAAGAGTAAC 
AAAGAGGAGT TTGCTAATGA ATTTCTGACG CTGAAAAGAT TATCCTCCAA GTATAAGGCA 
GATAAAACCT ATCCTACAAC TGTGGCTGAG AGGCCCAAGA ATATCAAGAA AAACAGATAT 
AAGGATATTT TGCCCTATGA CCATAGCCTA GTAGAACTGT GCATGATAAC GTCTGATGAG 
GATTCCAGCT ACATCAATGC CAACTTCATT AAGGGAGTTT ATGGACCCAA GACTTATATT 
GCCACCCAGG GACCTTTGCC TACAACTGTC CTGGACTTCT GGAGGATGAT TTGGGAATAC 
AGCATCCTGA TCATTGTTAT GGCGTGCATG GAGTTTGAAA TGGGAAAGAA AAAATGTGAG 
CGCTACTGGG CTGAACCAGG AGACACGCAA CTGCAACTTG GTCCTTTCTC CATATCCTGT 
GAAGCTGAAA ATAGAAAATC TGATTATACA ATCAGGACTC TAAAAGCCAA GTTCAATAGT 
GAAACTCGAA CCATCTATCA GTTCCATTAT AAGAACTGGC CAGACCATGA TGTGCCTTCA 
TCTATAGACC CTATTCTTGA GCTCATTTGG GATGTGCGTT GTTACCAAGA CGATAACAGT 
GTTCCCATAT GCATTCACTG CAGTGCTGGT TGTGGAAGGA CTGGTGTTCT CTGTGCTATT 
GATTACACGT GGATGTTGCT AAAAGATGGG ATAATTCCTG AGAACTTCAG TGTTTTTAGT 
TTGATCCAGG AAATGCGAAC ACAGAGGCCT TCATTAGTTC AAACTCAGTA TGAACTGGTC 
TACAACGCTG TATTAGAACT ATTTACGAGA CAGATGGATA TTATCGGGGA TAAACACTCT 
GGAACAGAGA TTCAAGCAAA GGATTCAGTT TTTGAGCAAA ATCCCAACAT AGAAGCAAAA 
TCTTATTCTC CTAGTTTACC AAAAAGTAAC ATAAAAGAAG CAACAGTGAT GAACCAACAG 
AGCAAAGGAA GGATAAGGGT GAAAAGTGCA GAAGCTTCTT CCTTTGACCT CAGGACTTCC 
GAAATAAATG AAAAGGAAGG GTTAGTCATG CACTCTGCTA AACAGAGCAG TTCTTTAGAG 
TTTTTGGAAC TGAACTGTGG TTGTAACAAA AATGCTGACA CAACCATGAA ATGGGAGACA 
AAGGTATTCC CAATAGCTGG GGAGCCTCTT CAGAAGCATC AAAGTTTGGA CTTGAGTTCC 
ATTTTGTTTG GAGCATATCC TAAATCTAAA CCTGTAAATG CAGCAGGAAG ATATTCTAAT 
TTAAAGAAGC CAATACTGCG GACCAAATCA ACTCCCTTTG AATTGATGCA AGAGAGAGAC 
AGCAAAGAGT TGGACATAAA GGGAAATTTT TATTTGGAAT CTCAACCACA CAATTCTTGT 
GTTGATCAGG CTCAAAAAGT GATGCATGTT TCTACAGCAG AACTGAATTT TTCACCATTA 
CATGACTCTA AGCATCAAAC ATGCAATGCT GCTAATGCAA AGCAGCATGA CTCTGGTGCT 
TTAAATGAGC ATTCTTATAT GTCTTTAGTA GAAGATCCTT ATTTTTCATC ATTGTCTCCA 
AGTAGTGCTC ATTCTAAGAC GTCTTTTAAT TTACCTCAGA AGCAAGATGG AAGTGTTTTA 
CCTTGTTCTT CACTGTCAAA ATCCTCTACA ACCCTCTTTC CTTATTACAA TTCACATGAT 
TCTTTAACAC TGAGTACTCT GACCAACTTT CCCCCACCAC TGAACCAAGA GACAGCAGCA 
GTAGCAACTT CTCCAAGGAT AGATGATGAA ATCCCTCCTC CACTTCCTGA ACGGACACCT 
GAATCTTTTA TTGTAGTAGA GGAAGCCGGA GAACTCCCAC AGGGTGTTCC CACATCTTCA 
TCTTCACCTG TGAAGGTAAA AATTGGAACA TCACTAGAAT GCAGTGGAAC ATCTGAATCA 
AAGAAATCTG ATGATTTGAG TGTAAAACCC CGGAGTCCAA AATCAGATCT ACAACAAGAT 
CGTTCTTCTT CCCCACCACC TCCACTGCCA GAGAGAACCT TAGAGTCCTT CCTTCTTGCT 
GATGAAGATT GTACACAGGC TCAACCTGTA GAGACTTGTT CTACGAGCTG TCCTAACACC 
ATGGAAAATT CAACATCTTC AAAACAGACA CTGAAACCTC CTGGAAAGAA TTTTACAAGG 
AGTAAGAGTT TGAAAATTTT ACGAAACGTG AAAAAGAGTA TCTGTAATTC TTCCCCACCA 
AACAAGCCTG CAGAGACTGC TCGGTCAAAA AACTCCAGCT CCTTTTTGAA TTTTGGTTTT 
GCAAATCGTT TTTCAAAACC CAAAGGACCA AGGAACCCAC CATCAACTTG GAAAATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024844653.1
CDS141..2540
Translation

Target ORF information:

RefSeq Version XM_024988885.1
Organism Bos taurus(cattle)
Definition Bos taurus protein tyrosine phosphatase, non-receptor type 22 (PTPN22), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024988885.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGACCAAA GAGAAATTCT GCAGAAGTTC TTGGATGAGG CACAACACAG GAAGAGTAAC 
AAAGAGGAGT TTGCTAATGA ATTTCTGACG CTGAAAAGAT TATCCTCCAA GTATAAGGCA 
GATAAAACCT ATCCTACAAC TGTGGCTGAG AGGCCCAAGA ATATCAAGAA AAACAGATAT 
AAGGATATTT TGCCCTATGA CCATAGCCTA GTAGAACTGT GCATGATAAC GTCTGATGAG 
GATTCCAGCT ACATCAATGC CAACTTCATT AAGGGAGTTT ATGGACCCAA GACTTATATT 
GCCACCCAGG GACCTTTGCC TACAACTGTC CTGGACTTCT GGAGGATGAT TTGGGAATAC 
AGCATCCTGA TCATTGTTAT GGCGTGCATG GAGTTTGAAA TGGGAAAGAA AAAATGTGAG 
CGCTACTGGG CTGAACCAGG AGACACGCAA CTGCAACTTG GTCCTTTCTC CATATCCTGT 
GAAGCTGAAA ATAGAAAATC TGATTATACA ATCAGGACTC TAAAAGCCAA GTTCAATAGT 
GAAACTCGAA CCATCTATCA GTTCCATTAT AAGAACTGGC CAGACCATGA TGTGCCTTCA 
TCTATAGACC CTATTCTTGA GCTCATTTGG GATGTGCGTT GTTACCAAGA CGATAACAGT 
GTTCCCATAT GCATTCACTG CAGTGCTGGT TGTGGAAGGA CTGGTGTTCT CTGTGCTATT 
GATTACACGT GGATGTTGCT AAAAGATGGG ATAATTCCTG AGAACTTCAG TGTTTTTAGT 
TTGATCCAGG AAATGCGAAC ACAGAGGCCT TCATTAGTTC AAACTCAGTA TGAACTGGTC 
TACAACGCTG TATTAGAACT ATTTACGAGA CAGATGGATA TTATCGGGGA TAAACACTCT 
GGAACAGAGA TTCAAGCAAA GGATTCAGTT TTTGAGCAAA ATCCCAACAT AGAAGCAAAA 
TCTTATTCTC CTAGTTTACC AAAAAGTAAC ATAAAAGAAG CAACAGTGAT GAACCAACAG 
AGCAAAGGAA GGATAAGGGT GAAAAGTGCA GAAGCTTCTT CCTTTGACCT CAGGACTTCC 
GAAATAAATG AAAAGGAAGG GTTAGTCATG CACTCTGCTA AACAGAGCAG TTCTTTAGAG 
TTTTTGGAAC TGAACTGTGG TTGTAACAAA AATGCTGACA CAACCATGAA ATGGGAGACA 
AAGGTATTCC CAATAGCTGG GGAGCCTCTT CAGAAGCATC AAAGTTTGGA CTTGAGTTCC 
ATTTTGTTTG GAGCATATCC TAAATCTAAA CCTGTAAATG CAGCAGGAAG ATATTCTAAT 
TTAAAGAAGC CAATACTGCG GACCAAATCA ACTCCCTTTG AATTGATGCA AGAGAGAGAC 
AGCAAAGAGT TGGACATAAA GGGAAATTTT TATTTGGAAT CTCAACCACA CAATTCTTGT 
GTTGATCAGG CTCAAAAAGT GATGCATGTT TCTACAGCAG AACTGAATTT TTCACCATTA 
CATGACTCTA AGCATCAAAC ATGCAATGCT GCTAATGCAA AGCAGCATGA CTCTGGTGCT 
TTAAATGAGC ATTCTTATAT GTCTTTAGTA GAAGATCCTT ATTTTTCATC ATTGTCTCCA 
AGTAGTGCTC ATTCTAAGAC GTCTTTTAAT TTACCTCAGA AGCAAGATGG AAGTGTTTTA 
CCTTGTTCTT CACTGTCAAA ATCCTCTACA ACCCTCTTTC CTTATTACAA TTCACATGAT 
TCTTTAACAC TGAGTACTCT GACCAACTTT CCCCCACCAC TGAACCAAGA GACAGCAGCA 
GTAGCAACTT CTCCAAGGAT AGATGATGAA ATCCCTCCTC CACTTCCTGA ACGGACACCT 
GAATCTTTTA TTGTAGTAGA GGAAGCCGGA GAACTCCCAC AGGGTGTTCC CACATCTTCA 
TCTTCACCTG TGAAGGTAAA AATTGGAACA TCACTAGAAT GCAGTGGAAC ATCTGAATCA 
AAGAAATCTG ATGATTTGAG TGTAAAACCC CGGAGTCCAA AATCAGATCT ACAACAAGAT 
CGTTCTTCTT CCCCACCACC TCCACTGCCA GAGAGAACCT TAGAGTCCTT CCTTCTTGCT 
GATGAAGATT GTACACAGGC TCAACCTGTA GAGACTTGTT CTACGAGCTG TCCTAACACC 
ATGGAAAATT CAACATCTTC AAAACAGACA CTGAAACCTC CTGGAAAGAA TTTTACAAGG 
AGTAAGAGTT TGAAAATTTT ACGAAACGTG AAAAAGAGTA TCTGTAATTC TTCCCCACCA 
AACAAGCCTG CAGAGACTGC TCGGTCAAAA AACTCCAGCT CCTTTTTGAA TTTTGGTTTT 
GCAAATCGTT TTTCAAAACC CAAAGGACCA AGGAACCCAC CATCAACTTG GAAAATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL