NCAN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCAN
Entrez Gene ID	523701
Full Name	neurocan
General protein information	Preferred Names neurocan Names neurocan core protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005208460.3	XP_005208517.1	neurocan core protein isoform X2
XM_015471872.2	XP_015327358.1	neurocan core protein isoform X1
XM_005208460.4	XP_005208517.1	neurocan core protein isoform X2
XM_015471872.1	XP_015327358.1	neurocan core protein isoform X1
NM_001193082.1	NP_001180011.1	neurocan core protein precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCAN	NP_004377.2
Mus musculus (house mouse)	Ncan	NP_031815.2
Canis lupus familiaris (dog)	NCAN	XP_541924.3
Bos taurus (cattle)	NCAN	NP_001180011.1
Rattus norvegicus (Norway rat)	Ncan	NP_113841.2
Pan troglodytes (chimpanzee)	NCAN	NP_001029342.1
	NCAN	XP_002808228.1
Rattus norvegicus (Norway rat)	LOC102550316	XP_006253042.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCAN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCAN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40865	NM_001193082.1 Latest version!	Bos taurus neurocan (NCAN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20801	XM_015471872.2 Latest version!	Bos taurus neurocan (NCAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20802	XM_005208460.4 Latest version!	Bos taurus neurocan (NCAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa20802	XM_005208460.3	Bos taurus neurocan (NCAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20801	XM_015471872.1	Bos taurus neurocan (NCAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCAN ( NM_001193082.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180011.1 Bos taurus neurocan (NCAN), mRNA.

Price & Availability↑

CloneID	OBa40865
Clone ID Related Accession (Same CDS sequence)	NM_001193082.1
Accession Version	NM_001193082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	neurocan core protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Jul 17, 2010 this sequence version replaced XM_867414.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145419, SAMN03145426 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGCCT TGTCTGTCTG GGTCTCAGGC CTCCTGACTC TCCAGATGCT TCTCTTTGTG 
GCTGGGGAGC AGGGCCCACA GCACACCACC GCAGCTGCAG CCACTGCTGA GAAGGGGCTC 
CACATGCAGA AAGTGGGGTC TGGGTCAGTC CGGGCTGCCC TGGCAGAGTT GGTGGCCCTG 
CCCTGTCTCT TTAGCCTGCG GCCACGGCCA GGTGCAGCTC GAGAAGCCCC ACGGATCAAA 
TGGACCAAGG TGCGGACTGC ATCAGGCCAG CGGCAGGACT TGCCCATCCT AGTAGCCAAG 
GACAACGTGG TCCGAGTCGC CAAGGGCTGG CAGGGACGTG TGTCACTTCC CGCCTACCCC 
CGGCACCGGG TCAACGCCAC GTTGCTGCTG GGACCACTGA GGGCCAGCGA CTCCGGGCTC 
TACCGCTGCC AGGTGGTGAG GGGCATCGAG GATGAGCAGG ACCTGGTGCC CCTGGAGGTG 
ACGGGTGTTG TGTTCCACTA CCGAGCAGCC CTGGATCGCT ACTCTCTAAC TTTTGCTGAG 
GCTCAGGAGG CCTGTCTTCT CAACTCAGCC ACCATTGCAG CCCCGCGGCA CCTGCAGGCT 
GCCTTTGAGG ACGGCTTTGA CAACTGCGAT GCCGGGTGGC TTTCTGACCG CACTGTTCGG 
TATCCTATCA CCCAGTCCCG TCCTGGTTGC TATGGTGACC GTAGCAGCCT TCCGGGGGTG 
AGGAACTATG GGAGGCGAGA CCCACGGGAA CTCTACGATG TGTATTGCTT TGCCCGTGAG 
CTGGGGGGCG AGGTCTTCTA CGTAGGCCTG GCCCGCCGCC TGACACTGGC CGGCGCACGT 
GCCCAGTGCC GACGCCAGGG CGCCGTGCTG GCCTCGGTAG GACAGCTGCA CCTGGCCTGG 
CATGAGGGCC TGGACCAGTG CGACCCGGGC TGGCTGGCCG ACGGCAGCGT GCGCTACCCG 
ATCCAGACGC CGCGTCGGCG CTGCGGTGGC CCAGCCCCTG GCGTGCGCAC CGTATACCGC 
TTTGCCAACC GCACCGGCTT CCCGGCACCT GGAGAGCGCT TCGATGCCTA CTGCTTCAGA 
GCTCATCACC CCACATCCCA ACATGGAGAC CCAGAGACCC CCTCATCTGG AGATGAGGGG 
GAGATTCTGT CGGCAGAAGG GCCCCCCGCT GGAGAATTGG AGCCCAGTCT GCAGGAGGAG 
GAGGAGGTAG TCACCCCGGA CATCCAGGAG CCTCTGGTGT CTAGTGGGGA AGAAGAGTCT 
CTGATCTTGG CAGAGAAGCA GGAATCTCAA GCGACCCCCA GCCGTGCCCC TGGGGTCCCC 
ACATTGGCCT CAAGTCCTGC CCTGGAGGCT GAGGAGGTGT GGCTGAGCAC AGTGGCCCCC 
AGCCACAGCA GCGTGGAGGC AGGCACTGTG GTGGGCACGC ACACAGACAC AGCCCCAGCA 
AGCACCACGC CCAGGAAGAG GGAGCGCTTT AAAGGGCTGA ACGGGCGCCA CTTCCAGCAA 
CAGGAGCCCC AGCAAGGGCT GAAGGGGGCA CTCGAGGCCA GCATCCAGCC CCCCACACCA 
GAGGCTGCTG AGAATTATGT GGAGCCTCCC ATGACCACTG GAACCACAGA CGCCTTGGGG 
AGCGGCCAGA GCCAAAGCCC CTGGGCTGTG CTGACAAATG AGGTGGAAAT GCCCAGAGCT 
GGTTCCCTTG GTGGCAGGAG CCCCCAAGAG CCCTGGCTGT GGCTCCCAAC CGTGGTCCCA 
CCCATCATCC CAGACCCCAG CAGAGCCCTT GGCCTGGAAC TAGGAGAAGC CAAGCGCTTT 
AGTATGAGAC CAGTCACTTC CGACCTGCCC TGGTCCCCCT CAGAGACCAC TCCCAACCCT 
TCCAATGGCC CCAGCCCTAC CCCCTGGAAG GCTTCTCCTG TGGCCATTTC TCCAGACCTC 
CCTGTCATGG CCATGCTTCG CGCCCCCAAA CTGTGGCTCC TATCACAGGA GCCTCCTACA 
TCCCTCCCCA CTGAGGCCAG TGGAGTCAAG GGGCATAGTG AGGCCATGGC CACTGCCCTG 
CCCTCCCCTG CCTCAGAAAT CGAAGCCAGC CCACAGGATC CCATCCATCT GGAAGTGTAC 
TCCCCGCCTT CCTCCTCGGG CCTGGCAGGA CAAGATGGAG AGTCCACATC CCTGTCACTC 
AGCACCAGTC CCAACTCCCC TGGAGCAGAC TTTGGAAACA CTAGAGGGGC CAGCCCTACT 
GAGCTCAGCA AAGCTGAGCA CCCCGGATCC AGCCCACAGG CCTCTGTGGA CTGGAATGTG 
GTGGCAGATA TCACTATTTC TGAGCCTGCC ACTGAGCCCA TGGGAGCCAG AGGCGTCTCG 
GAGTCTGAGT CTGGGGTCTT CAACACAGCA GAGAGTCCTA CTTCCAGCTT GCAGGCCTCC 
ATGGACAAGA CGCAGGAAAT ATGGGCCTCG CTGCACAGGG AAGGGCTGGA CCCCCACTCC 
CCATCTGCAC CCTTGGGGGT CCCTGAAGTC CCCTTGATGC CCAAGTTCAC CCCAGGTTTG 
GGGCCTTGGG CTGCTACAGA TGGAGGAGTC ACAGTGGATC CTATGGCTTC CACAGTCACA 
CTGGACACCA GCGATGCTGG TGGGCATTGG GAACCTGGAT CCCACATGGT TGAGGGAGCA 
GAAAGCCCCA CCTTGAGCCC TCAAATGGCT GTGGACACAA GTGTGGTGAC ATCCGTCACG 
TCCTTGGACC TGGGGGACAA GGTCAGGGTC CTGGCCGTGT TCACAGTGGC CTCCTCAAGC 
TCCCAGCCCC ACCCAGAGCC AGAGAGCCAG CTGGTGACCC AGGGAACCCT GGGAGCCTTG 
GAGCCTCCAC AGGAGGGCAG CCACTCAGGG GACCCCATTC TTCCTCCTTG GACGCCGACA 
GCAGCCAACA AGGATGAGCC CATTTCAGTT TCCTCGGGGG AGCCTACAGT GCCTTGGGAC 
TCCCCCAGCA CCCTGCTGCC TGCCTCTCTG GGCCCAGAGG AATTTGAGCT GGAGGTCCTG 
ATGGGGAGCC CCAGTGTGGA GAGCTTCTGG GAGGAGGCAG CAAGTGGAGA GGAGCCTGCC 
CTGCCAGGGA CTCCTGCAAA TGGGAGTGCA GAGGAGGGCC CCTTGGATCC CTGTGAGAAC 
AACCCTTGCC TGCACGGAGG CACCTGTAGA GCCAATGGCA CCATGTACGG CTGCAGCTGT 
GACCAGGGGT TCGCTGGAGA GAACTGTGAG ATCGATATTG ACGATTGCAT CTCCAGCCCC 
TGTGAGAACG GTGGCACCTG CATCGACGAG GTCAACGCCT TCATCTGCCT TTGCCTCCCC 
AGCTATGGGG GAAGCCTCTG CGAGAAAGAT ACAGAGGGCT GTGACCACGG CTGGCACAAG 
TTTCAGGGCC ACTGCTACCG TTACTTTGCC CACCGGCGGG CATGGGAGGA CGCCGAGAGG 
GACTGCCGCC GCCGAGCCGG CCACCTAACC AGCATCCACT CATCAGAAGA ACACAACTTC 
ATTAACAGTT TTGGGCGTGA AAACACATGG ATCGGCTTGA ACGACAGGAT CGTGGAAAGG 
GATTTCCAGT GGACGGACAA CACAGGGCTG CAATTTGAGA ACTGGCGGGA AAATCAGCCG 
GACAACTTCT TCGCGGGCGG GGAGGACTGT GTGGTGATGG TTGCACATGA GAGCGGGCGC 
TGGAACGACG TTCCCTGCAA CTACAACCTC CCCTACGTCT GTAAGAAGGG CACAGTGCTG 
TGTGGTCCCC CTCCAGCGGT GGAGAATGCC TCGCCCATTG GTGCCCGCAA GGCCAAGTAT 
AATGTCCATG CCACAGTACG CTACCAGTGT GATGAAGGAT TTGCCCAGCA CCATGTGGCC 
ATCATCCGGT GCCGGAGCAA TGGCAAATGG GACCGGCCCC AGATCATCTG CACCAAACCC 
AGAAGGTCCC ATAGGATGCG GCGACACCAC CATCACCACC AGCATCACCA CCAGCATCGC 
CACCACAAAT CTCGCAAGGA GCGCAGAAAA CACAAGAAGC ACCCAGCAGA GGACTGGGAA 
AAGGAAGAAG GGAATTTCTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180011.1
CDS	99..4142
Translation	MGALSVWVSGLLTLQMLLFVAGEQGPQHTTAAAATAEKGLHMQKVGSGSVRAALAELVALPCLFSLRPRPGAAREAPRIKWTKVRTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRVNATLLLGPLRASDSGLYRCQVVRGIEDEQDLVPLEVTGVVFHYRAALDRYSLTFAEAQEACLLNSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRNYGRRDPRELYDVYCFARELGGEVFYVGLARRLTLAGARAQCRRQGAVLASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGERFDAYCFRAHHPTSQHGDPETPSSGDEGEILSAEGPPAGELEPSLQEEEEVVTPDIQEPLVSSGEEESLILAEKQESQATPSRAPGVPTLASSPALEAEEVWLSTVAPSHSSVEAGTVVGTHTDTAPASTTPRKRERFKGLNGRHFQQQEPQQGLKGALEASIQPPTPEAAENYVEPPMTTGTTDALGSGQSQSPWAVLTNEVEMPRAGSLGGRSPQEPWLWLPTVVPPIIPDPSRALGLELGEAKRFSMRPVTSDLPWSPSETTPNPSNGPSPTPWKASPVAISPDLPVMAMLRAPKLWLLSQEPPTSLPTEASGVKGHSEAMATALPSPASEIEASPQDPIHLEVYSPPSSSGLAGQDGESTSLSLSTSPNSPGADFGNTRGASPTELSKAEHPGSSPQASVDWNVVADITISEPATEPMGARGVSESESGVFNTAESPTSSLQASMDKTQEIWASLHREGLDPHSPSAPLGVPEVPLMPKFTPGLGPWAATDGGVTVDPMASTVTLDTSDAGGHWEPGSHMVEGAESPTLSPQMAVDTSVVTSVTSLDLGDKVRVLAVFTVASSSSQPHPEPESQLVTQGTLGALEPPQEGSHSGDPILPPWTPTAANKDEPISVSSGEPTVPWDSPSTLLPASLGPEEFELEVLMGSPSVESFWEEAASGEEPALPGTPANGSAEEGPLDPCENNPCLHGGTCRANGTMYGCSCDQGFAGENCEIDIDDCISSPCENGGTCIDEVNAFICLCLPSYGGSLCEKDTEGCDHGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSIHSSEEHNFINSFGRENTWIGLNDRIVERDFQWTDNTGLQFENWRENQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASPIGARKAKYNVHATVRYQCDEGFAQHHVAIIRCRSNGKWDRPQIICTKPRRSHRMRRHHHHHQHHHQHRHHKSRKERRKHKKHPAEDWEKEEGNFC

Target ORF information:

RefSeq Version	NM_001193082.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurocan (NCAN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193082.1

ORF Insert Sequence:

ATGGGGGCCT TGTCTGTCTG GGTCTCAGGC CTCCTGACTC TCCAGATGCT TCTCTTTGTG 
GCTGGGGAGC AGGGCCCACA GCACACCACC GCAGCTGCAG CCACTGCTGA GAAGGGGCTC 
CACATGCAGA AAGTGGGGTC TGGGTCAGTC CGGGCTGCCC TGGCAGAGTT GGTGGCCCTG 
CCCTGTCTCT TTAGCCTGCG GCCACGGCCA GGTGCAGCTC GAGAAGCCCC ACGGATCAAA 
TGGACCAAGG TGCGGACTGC ATCAGGCCAG CGGCAGGACT TGCCCATCCT AGTAGCCAAG 
GACAACGTGG TCCGAGTCGC CAAGGGCTGG CAGGGACGTG TGTCACTTCC CGCCTACCCC 
CGGCACCGGG TCAACGCCAC GTTGCTGCTG GGACCACTGA GGGCCAGCGA CTCCGGGCTC 
TACCGCTGCC AGGTGGTGAG GGGCATCGAG GATGAGCAGG ACCTGGTGCC CCTGGAGGTG 
ACGGGTGTTG TGTTCCACTA CCGAGCAGCC CTGGATCGCT ACTCTCTAAC TTTTGCTGAG 
GCTCAGGAGG CCTGTCTTCT CAACTCAGCC ACCATTGCAG CCCCGCGGCA CCTGCAGGCT 
GCCTTTGAGG ACGGCTTTGA CAACTGCGAT GCCGGGTGGC TTTCTGACCG CACTGTTCGG 
TATCCTATCA CCCAGTCCCG TCCTGGTTGC TATGGTGACC GTAGCAGCCT TCCGGGGGTG 
AGGAACTATG GGAGGCGAGA CCCACGGGAA CTCTACGATG TGTATTGCTT TGCCCGTGAG 
CTGGGGGGCG AGGTCTTCTA CGTAGGCCTG GCCCGCCGCC TGACACTGGC CGGCGCACGT 
GCCCAGTGCC GACGCCAGGG CGCCGTGCTG GCCTCGGTAG GACAGCTGCA CCTGGCCTGG 
CATGAGGGCC TGGACCAGTG CGACCCGGGC TGGCTGGCCG ACGGCAGCGT GCGCTACCCG 
ATCCAGACGC CGCGTCGGCG CTGCGGTGGC CCAGCCCCTG GCGTGCGCAC CGTATACCGC 
TTTGCCAACC GCACCGGCTT CCCGGCACCT GGAGAGCGCT TCGATGCCTA CTGCTTCAGA 
GCTCATCACC CCACATCCCA ACATGGAGAC CCAGAGACCC CCTCATCTGG AGATGAGGGG 
GAGATTCTGT CGGCAGAAGG GCCCCCCGCT GGAGAATTGG AGCCCAGTCT GCAGGAGGAG 
GAGGAGGTAG TCACCCCGGA CATCCAGGAG CCTCTGGTGT CTAGTGGGGA AGAAGAGTCT 
CTGATCTTGG CAGAGAAGCA GGAATCTCAA GCGACCCCCA GCCGTGCCCC TGGGGTCCCC 
ACATTGGCCT CAAGTCCTGC CCTGGAGGCT GAGGAGGTGT GGCTGAGCAC AGTGGCCCCC 
AGCCACAGCA GCGTGGAGGC AGGCACTGTG GTGGGCACGC ACACAGACAC AGCCCCAGCA 
AGCACCACGC CCAGGAAGAG GGAGCGCTTT AAAGGGCTGA ACGGGCGCCA CTTCCAGCAA 
CAGGAGCCCC AGCAAGGGCT GAAGGGGGCA CTCGAGGCCA GCATCCAGCC CCCCACACCA 
GAGGCTGCTG AGAATTATGT GGAGCCTCCC ATGACCACTG GAACCACAGA CGCCTTGGGG 
AGCGGCCAGA GCCAAAGCCC CTGGGCTGTG CTGACAAATG AGGTGGAAAT GCCCAGAGCT 
GGTTCCCTTG GTGGCAGGAG CCCCCAAGAG CCCTGGCTGT GGCTCCCAAC CGTGGTCCCA 
CCCATCATCC CAGACCCCAG CAGAGCCCTT GGCCTGGAAC TAGGAGAAGC CAAGCGCTTT 
AGTATGAGAC CAGTCACTTC CGACCTGCCC TGGTCCCCCT CAGAGACCAC TCCCAACCCT 
TCCAATGGCC CCAGCCCTAC CCCCTGGAAG GCTTCTCCTG TGGCCATTTC TCCAGACCTC 
CCTGTCATGG CCATGCTTCG CGCCCCCAAA CTGTGGCTCC TATCACAGGA GCCTCCTACA 
TCCCTCCCCA CTGAGGCCAG TGGAGTCAAG GGGCATAGTG AGGCCATGGC CACTGCCCTG 
CCCTCCCCTG CCTCAGAAAT CGAAGCCAGC CCACAGGATC CCATCCATCT GGAAGTGTAC 
TCCCCGCCTT CCTCCTCGGG CCTGGCAGGA CAAGATGGAG AGTCCACATC CCTGTCACTC 
AGCACCAGTC CCAACTCCCC TGGAGCAGAC TTTGGAAACA CTAGAGGGGC CAGCCCTACT 
GAGCTCAGCA AAGCTGAGCA CCCCGGATCC AGCCCACAGG CCTCTGTGGA CTGGAATGTG 
GTGGCAGATA TCACTATTTC TGAGCCTGCC ACTGAGCCCA TGGGAGCCAG AGGCGTCTCG 
GAGTCTGAGT CTGGGGTCTT CAACACAGCA GAGAGTCCTA CTTCCAGCTT GCAGGCCTCC 
ATGGACAAGA CGCAGGAAAT ATGGGCCTCG CTGCACAGGG AAGGGCTGGA CCCCCACTCC 
CCATCTGCAC CCTTGGGGGT CCCTGAAGTC CCCTTGATGC CCAAGTTCAC CCCAGGTTTG 
GGGCCTTGGG CTGCTACAGA TGGAGGAGTC ACAGTGGATC CTATGGCTTC CACAGTCACA 
CTGGACACCA GCGATGCTGG TGGGCATTGG GAACCTGGAT CCCACATGGT TGAGGGAGCA 
GAAAGCCCCA CCTTGAGCCC TCAAATGGCT GTGGACACAA GTGTGGTGAC ATCCGTCACG 
TCCTTGGACC TGGGGGACAA GGTCAGGGTC CTGGCCGTGT TCACAGTGGC CTCCTCAAGC 
TCCCAGCCCC ACCCAGAGCC AGAGAGCCAG CTGGTGACCC AGGGAACCCT GGGAGCCTTG 
GAGCCTCCAC AGGAGGGCAG CCACTCAGGG GACCCCATTC TTCCTCCTTG GACGCCGACA 
GCAGCCAACA AGGATGAGCC CATTTCAGTT TCCTCGGGGG AGCCTACAGT GCCTTGGGAC 
TCCCCCAGCA CCCTGCTGCC TGCCTCTCTG GGCCCAGAGG AATTTGAGCT GGAGGTCCTG 
ATGGGGAGCC CCAGTGTGGA GAGCTTCTGG GAGGAGGCAG CAAGTGGAGA GGAGCCTGCC 
CTGCCAGGGA CTCCTGCAAA TGGGAGTGCA GAGGAGGGCC CCTTGGATCC CTGTGAGAAC 
AACCCTTGCC TGCACGGAGG CACCTGTAGA GCCAATGGCA CCATGTACGG CTGCAGCTGT 
GACCAGGGGT TCGCTGGAGA GAACTGTGAG ATCGATATTG ACGATTGCAT CTCCAGCCCC 
TGTGAGAACG GTGGCACCTG CATCGACGAG GTCAACGCCT TCATCTGCCT TTGCCTCCCC 
AGCTATGGGG GAAGCCTCTG CGAGAAAGAT ACAGAGGGCT GTGACCACGG CTGGCACAAG 
TTTCAGGGCC ACTGCTACCG TTACTTTGCC CACCGGCGGG CATGGGAGGA CGCCGAGAGG 
GACTGCCGCC GCCGAGCCGG CCACCTAACC AGCATCCACT CATCAGAAGA ACACAACTTC 
ATTAACAGTT TTGGGCGTGA AAACACATGG ATCGGCTTGA ACGACAGGAT CGTGGAAAGG 
GATTTCCAGT GGACGGACAA CACAGGGCTG CAATTTGAGA ACTGGCGGGA AAATCAGCCG 
GACAACTTCT TCGCGGGCGG GGAGGACTGT GTGGTGATGG TTGCACATGA GAGCGGGCGC 
TGGAACGACG TTCCCTGCAA CTACAACCTC CCCTACGTCT GTAAGAAGGG CACAGTGCTG 
TGTGGTCCCC CTCCAGCGGT GGAGAATGCC TCGCCCATTG GTGCCCGCAA GGCCAAGTAT 
AATGTCCATG CCACAGTACG CTACCAGTGT GATGAAGGAT TTGCCCAGCA CCATGTGGCC 
ATCATCCGGT GCCGGAGCAA TGGCAAATGG GACCGGCCCC AGATCATCTG CACCAAACCC 
AGAAGGTCCC ATAGGATGCG GCGACACCAC CATCACCACC AGCATCACCA CCAGCATCGC 
CACCACAAAT CTCGCAAGGA GCGCAGAAAA CACAAGAAGC ACCCAGCAGA GGACTGGGAA 
AAGGAAGAAG GGAATTTCTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCAN ( XM_015471872.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327358.1 Bos taurus neurocan (NCAN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20801
Clone ID Related Accession (Same CDS sequence)	XM_015471872.2 , XM_015471872.1
Accession Version	XM_015471872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurocan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCAT GCTCTGACCA TCAGACTTCA CTTGCTGGGG TTCTGAAGTC CAGGATGGGG 
GCCTTGTCTG TCTGGGTCTC AGGCCTCCTG ACTCTCCAGA TGCTTCTCTT TGTGGCTGGG 
GAGCAGGGCC CACAGCACAC CACCGCAGCT GCAGCCACTG CTGAGAAGGG GCTCCACATG 
CAGAAAGTGG GGTCTGGGTC AGTCCGGGCT GCCCTGGCAG AGTTGGTGGC CCTGCCCTGT 
CTCTTTAGCC TGCGGCCACG GCCAGGTGCA GCTCGAGAAG CCCCACGGAT CAAATGGACC 
AAGGTGCGGA CTGCATCAGG CCAGCGGCAG GACTTGCCCA TCCTAGTAGC CAAGGACAAC 
GTGGTCCGAG TCGCCAAGGG CTGGCAGGGA CGTGTGTCAC TTCCCGCCTA CCCCCGGCAC 
CGGGTCAACG CCACGTTGCT GCTGGGACCA CTGAGGGCCA GCGACTCCGG GCTCTACCGC 
TGCCAGGTGG TGAGGGGCAT CGAGGATGAG CAGGACCTGG TGCCCCTGGA GGTGACGGGT 
GTTGTGTTCC ACTACCGAGC AGCCCTGGAT CGCTACTCTC TAACTTTTGC TGAGGCTCAG 
GAGGCCTGTC TTCTCAACTC AGCCACCATT GCAGCCCCGC GGCACCTGCA GGCTGCCTTT 
GAGGACGGCT TTGACAACTG CGATGCCGGG TGGCTTTCTG ACCGCACTGT TCGGTATCCT 
ATCACCCAGT CCCGTCCTGG TTGCTATGGT GACCGTAGCA GCCTTCCGGG GGTGAGGAAC 
TATGGGAGGC GAGACCCACG GGAACTCTAC GATGTGTATT GCTTTGCCCG TGAGCTGGGG 
GGCGAGGTCT TCTACGTAGG CCTGGCCCGC CGCCTGACAC TGGCCGGCGC ACGTGCCCAG 
TGCCGACGCC AGGGCGCCGT GCTGGCCTCG GTAGGACAGC TGCACCTGGC CTGGCATGAG 
GGCCTGGACC AGTGCGACCC GGGCTGGCTG GCCGACGGCA GCGTGCGCTA CCCGATCCAG 
ACGCCGCGTC GGCGCTGCGG TGGCCCAGCC CCTGGCGTGC GCACCGTATA CCGCTTTGCC 
AACCGCACCG GCTTCCCGGC ACCTGGAGAG CGCTTCGATG CCTACTGCTT CAGAGCTCAT 
CACCCCACAT CCCAACATGG AGACCCAGAG ACCCCCTCAT CTGGAGATGA GGGGGAGATT 
CTGTCGGCAG AAGGGCCCCC CGCTGGAGAA TTGGAGCCCA GTCTGCAGGA GGAGGAGGAG 
GTAGTCACCC CGGACATCCA GGAGCCTCTG GTGTCTAGTG GGGAAGAAGA GTCTCTGATC 
TTGGCAGAGA AGCAGGAATC TCAAGCGACC CCCAGCCGTG CCCCTGGGGT CCCCACATTG 
GCCTCAAGTC CTGCCCTGGA GGCTGAGGAG GTGTGGCTGA GCACAGTGGC CCCCAGCCAC 
AGCAGCGTGG AGGCAGGCAC TGTGGTGGGC ACGCACACAG ACACAGCCCC AGCAAGCACC 
ACGCCCAGGA AGAGGGAGCG CTTTAAAGGG CTGAACGGGC GCCACTTCCA GCAACAGGAG 
CCCCAGCAAG GGCTGAAGGG GGCACTCGAG GCCAGCATCC AGCCCCCCAC ACCAGAGGCT 
GCTGAGAATT ATGTGGAGCC TCCCATGACC ACTGGAACCA CAGACGCCTT GGGGAGCGGC 
CAGAGCCAAA GCCCCTGGGC TGTGCTGACA AATGAGGTGG AAATGCCCAG AGCTGGTTCC 
CTTGGTGGCA GGAGCCCCCA AGAGCCCTGG CTGTGGCTCC CAACCGTGGT CCCACCCATC 
ATCCCAGACC CCAGCAGAGC CCTTGGCCTG GAACTAGGAG AAGCCAAGCG CTTTAGTATG 
AGACCAGTCA CTTCCGACCT GCCCTGGTCC CCCTCAGAGA CCACTCCCAA CCCTTCCAAT 
GGCCCCAGCC CTACCCCCTG GAAGGCTTCT CCTGTGGCCA TTTCTCCAGA CCTCCCTGTC 
ATGGCCATGC TTCGCGCCCC CAAACTGTGG CTCCTATCAC AGGAGCCTCC TACATCCCTC 
CCCACTGAGG CCAGTGGAGT CAAGGGGCAT AGTGAGGCCA TGGCCACTGC CCTGCCCTCC 
CCTGCCTCAG AAATCGAAGC CAGCCCACAG GATCCCATCC ATCTGGAAGT GTACTCCCCG 
CCTTCCTCCT CGGGCCTGGC AGGACAAGAT GGAGAGTCCA CATCCCTGTC ACTCAGCAGC 
CACAGAGGAG GAAGTCCTCC GGCCCCTTCA CAGGCAGCCA CAGACACACA AGCTGGAGCC 
AGTCCCAACT CCCCTGGAGC AGACTTTGGA AACACTAGAG GGGCCAGCCC TACTGAGCTC 
AGCAAAGCTG AGCACCCCGG ATCCAGCCCA CAGGCCTCTG TGGACTGGAA TGTGGTGGCA 
GATATCACTA TTTCTGAGCC TGCCACTGAG CCCATGGGAG CCAGAGGCGT CTCGGAGTCT 
GAGTCTGGGG TCTTCAACAC AGCAGAGAGT CCTACTTCCA GCTTGCAGGC CTCCATGGAC 
AAGACGCAGG AAATATGGGC CTCGCTGCAC AGGGAAGGGC TGGACCCCCA CTCCCCATCT 
GCACCCTTGG GGGTCCCTGA AGTCCCCTTG ATGCCCAAGT TCACCCCAGG TTTGGGGCCT 
TGGGCTGCTA CAGATGGAGG AGTCACAGTG GATCCTATGG CTTCCACAGT CACACTGGAC 
ACCAGCGATG CTGGTGGGCA TTGGGAACCT GGATCCCACA TGGTTGAGGG AGCAGAAAGC 
CCCACCTTGA GCCCTCAAAT GGCTGTGGAC ACAAGTGTGG TGACATCCGT CACGTCCTTG 
GACCTGGGGG ACAAGGTCAG GGTCCTGGCC GTGTTCACAG TGGCCTCCTC AAGCTCCCAG 
CCCCACCCAG AGCCAGAGAG CCAGCTGGTG ACCCAGGGAA CCCTGGGAGC CTTGGAGCCT 
CCACAGGAGG GCAGCCACTC AGGGGACCCC ATTCTTCCTC CTTGGACGCC GACAGCAGCC 
AACAAGGATG AGCCCATTTC AGTTTCCTCG GGGGAGCCTA CAGTGCCTTG GGACTCCCCC 
AGCACCCTGC TGCCTGCCTC TCTGGGCCCA GAGGAATTTG AGCTGGAGGT CCTGATGGGG 
AGCCCCAGTG TGGAGAGCTT CTGGGAGGAG GCAGCAAGTG GAGAGGAGCC TGCCCTGCCA 
GGGACTCCTG CAAATGGGAG TGCAGAGGAG GGCCCCTTGG ATCCCTGTGA GAACAACCCT 
TGCCTGCACG GAGGCACCTG TAGAGCCAAT GGCACCATGT ACGGCTGCAG CTGTGACCAG 
GGGTTCGCTG GAGAGAACTG TGAGATCGAT ATTGACGATT GCATCTCCAG CCCCTGTGAG 
AACGGTGGCA CCTGCATCGA CGAGGTCAAC GCCTTCATCT GCCTTTGCCT CCCCAGCTAT 
GGGGGAAGCC TCTGCGAGAA AGATACAGAG GGCTGTGACC ACGGCTGGCA CAAGTTTCAG 
GGCCACTGCT ACCGTTACTT TGCCCACCGG CGGGCATGGG AGGACGCCGA GAGGGACTGC 
CGCCGCCGAG CCGGCCACCT AACCAGCATC CACTCATCAG AAGAACACAA CTTCATTAAC 
AGTTTTGGGC GTGAAAACAC ATGGATCGGC TTGAACGACA GGATCGTGGA AAGGGATTTC 
CAGTGGACGG ACAACACAGG GCTGCAATTT GAGAACTGGC GGGAAAATCA GCCGGACAAC 
TTCTTCGCGG GCGGGGAGGA CTGTGTGGTG ATGGTTGCAC ATGAGAGCGG GCGCTGGAAC 
GACGTTCCCT GCAACTACAA CCTCCCCTAC GTCTGTAAGA AGGGCACAGT GCTGTGTGGT 
CCCCCTCCAG CGGTGGAGAA TGCCTCGCCC ATTGGTGCCC GCAAGGCCAA GTATAATGTC 
CATGCCACAG TACGCTACCA GTGTGATGAA GGATTTGCCC AGCACCATGT GGCCATCATC 
CGGTGCCGGA GCAATGGCAA ATGGGACCGG CCCCAGATCA TCTGCACCAA ACCCAGAAGG 
TCCCATAGGA TGCGGCGACA CCACCATCAC CACCAGCATC ACCACCAGCA TCGCCACCAC 
AAATCTCGCA AGGAGCGCAG AAAACACAAG AAGCACCCAG CAGAGGACTG GGAAAAGGAA 
GAAGGGAATT TCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327358.1
CDS	74..4231
Translation	MHSCSDHQTSLAGVLKSRMGALSVWVSGLLTLQMLLFVAGEQGPQHTTAAAATAEKGLHMQKVGSGSVRAALAELVALPCLFSLRPRPGAAREAPRIKWTKVRTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRVNATLLLGPLRASDSGLYRCQVVRGIEDEQDLVPLEVTGVVFHYRAALDRYSLTFAEAQEACLLNSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRNYGRRDPRELYDVYCFARELGGEVFYVGLARRLTLAGARAQCRRQGAVLASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGERFDAYCFRAHHPTSQHGDPETPSSGDEGEILSAEGPPAGELEPSLQEEEEVVTPDIQEPLVSSGEEESLILAEKQESQATPSRAPGVPTLASSPALEAEEVWLSTVAPSHSSVEAGTVVGTHTDTAPASTTPRKRERFKGLNGRHFQQQEPQQGLKGALEASIQPPTPEAAENYVEPPMTTGTTDALGSGQSQSPWAVLTNEVEMPRAGSLGGRSPQEPWLWLPTVVPPIIPDPSRALGLELGEAKRFSMRPVTSDLPWSPSETTPNPSNGPSPTPWKASPVAISPDLPVMAMLRAPKLWLLSQEPPTSLPTEASGVKGHSEAMATALPSPASEIEASPQDPIHLEVYSPPSSSGLAGQDGESTSLSLSSHRGGSPPAPSQAATDTQAGASPNSPGADFGNTRGASPTELSKAEHPGSSPQASVDWNVVADITISEPATEPMGARGVSESESGVFNTAESPTSSLQASMDKTQEIWASLHREGLDPHSPSAPLGVPEVPLMPKFTPGLGPWAATDGGVTVDPMASTVTLDTSDAGGHWEPGSHMVEGAESPTLSPQMAVDTSVVTSVTSLDLGDKVRVLAVFTVASSSSQPHPEPESQLVTQGTLGALEPPQEGSHSGDPILPPWTPTAANKDEPISVSSGEPTVPWDSPSTLLPASLGPEEFELEVLMGSPSVESFWEEAASGEEPALPGTPANGSAEEGPLDPCENNPCLHGGTCRANGTMYGCSCDQGFAGENCEIDIDDCISSPCENGGTCIDEVNAFICLCLPSYGGSLCEKDTEGCDHGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSIHSSEEHNFINSFGRENTWIGLNDRIVERDFQWTDNTGLQFENWRENQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASPIGARKAKYNVHATVRYQCDEGFAQHHVAIIRCRSNGKWDRPQIICTKPRRSHRMRRHHHHHQHHHQHRHHKSRKERRKHKKHPAEDWEKEEGNFC

Target ORF information:

RefSeq Version	XM_015471872.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurocan (NCAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471872.2

ORF Insert Sequence:

ATGCACTCAT GCTCTGACCA TCAGACTTCA CTTGCTGGGG TTCTGAAGTC CAGGATGGGG 
GCCTTGTCTG TCTGGGTCTC AGGCCTCCTG ACTCTCCAGA TGCTTCTCTT TGTGGCTGGG 
GAGCAGGGCC CACAGCACAC CACCGCAGCT GCAGCCACTG CTGAGAAGGG GCTCCACATG 
CAGAAAGTGG GGTCTGGGTC AGTCCGGGCT GCCCTGGCAG AGTTGGTGGC CCTGCCCTGT 
CTCTTTAGCC TGCGGCCACG GCCAGGTGCA GCTCGAGAAG CCCCACGGAT CAAATGGACC 
AAGGTGCGGA CTGCATCAGG CCAGCGGCAG GACTTGCCCA TCCTAGTAGC CAAGGACAAC 
GTGGTCCGAG TCGCCAAGGG CTGGCAGGGA CGTGTGTCAC TTCCCGCCTA CCCCCGGCAC 
CGGGTCAACG CCACGTTGCT GCTGGGACCA CTGAGGGCCA GCGACTCCGG GCTCTACCGC 
TGCCAGGTGG TGAGGGGCAT CGAGGATGAG CAGGACCTGG TGCCCCTGGA GGTGACGGGT 
GTTGTGTTCC ACTACCGAGC AGCCCTGGAT CGCTACTCTC TAACTTTTGC TGAGGCTCAG 
GAGGCCTGTC TTCTCAACTC AGCCACCATT GCAGCCCCGC GGCACCTGCA GGCTGCCTTT 
GAGGACGGCT TTGACAACTG CGATGCCGGG TGGCTTTCTG ACCGCACTGT TCGGTATCCT 
ATCACCCAGT CCCGTCCTGG TTGCTATGGT GACCGTAGCA GCCTTCCGGG GGTGAGGAAC 
TATGGGAGGC GAGACCCACG GGAACTCTAC GATGTGTATT GCTTTGCCCG TGAGCTGGGG 
GGCGAGGTCT TCTACGTAGG CCTGGCCCGC CGCCTGACAC TGGCCGGCGC ACGTGCCCAG 
TGCCGACGCC AGGGCGCCGT GCTGGCCTCG GTAGGACAGC TGCACCTGGC CTGGCATGAG 
GGCCTGGACC AGTGCGACCC GGGCTGGCTG GCCGACGGCA GCGTGCGCTA CCCGATCCAG 
ACGCCGCGTC GGCGCTGCGG TGGCCCAGCC CCTGGCGTGC GCACCGTATA CCGCTTTGCC 
AACCGCACCG GCTTCCCGGC ACCTGGAGAG CGCTTCGATG CCTACTGCTT CAGAGCTCAT 
CACCCCACAT CCCAACATGG AGACCCAGAG ACCCCCTCAT CTGGAGATGA GGGGGAGATT 
CTGTCGGCAG AAGGGCCCCC CGCTGGAGAA TTGGAGCCCA GTCTGCAGGA GGAGGAGGAG 
GTAGTCACCC CGGACATCCA GGAGCCTCTG GTGTCTAGTG GGGAAGAAGA GTCTCTGATC 
TTGGCAGAGA AGCAGGAATC TCAAGCGACC CCCAGCCGTG CCCCTGGGGT CCCCACATTG 
GCCTCAAGTC CTGCCCTGGA GGCTGAGGAG GTGTGGCTGA GCACAGTGGC CCCCAGCCAC 
AGCAGCGTGG AGGCAGGCAC TGTGGTGGGC ACGCACACAG ACACAGCCCC AGCAAGCACC 
ACGCCCAGGA AGAGGGAGCG CTTTAAAGGG CTGAACGGGC GCCACTTCCA GCAACAGGAG 
CCCCAGCAAG GGCTGAAGGG GGCACTCGAG GCCAGCATCC AGCCCCCCAC ACCAGAGGCT 
GCTGAGAATT ATGTGGAGCC TCCCATGACC ACTGGAACCA CAGACGCCTT GGGGAGCGGC 
CAGAGCCAAA GCCCCTGGGC TGTGCTGACA AATGAGGTGG AAATGCCCAG AGCTGGTTCC 
CTTGGTGGCA GGAGCCCCCA AGAGCCCTGG CTGTGGCTCC CAACCGTGGT CCCACCCATC 
ATCCCAGACC CCAGCAGAGC CCTTGGCCTG GAACTAGGAG AAGCCAAGCG CTTTAGTATG 
AGACCAGTCA CTTCCGACCT GCCCTGGTCC CCCTCAGAGA CCACTCCCAA CCCTTCCAAT 
GGCCCCAGCC CTACCCCCTG GAAGGCTTCT CCTGTGGCCA TTTCTCCAGA CCTCCCTGTC 
ATGGCCATGC TTCGCGCCCC CAAACTGTGG CTCCTATCAC AGGAGCCTCC TACATCCCTC 
CCCACTGAGG CCAGTGGAGT CAAGGGGCAT AGTGAGGCCA TGGCCACTGC CCTGCCCTCC 
CCTGCCTCAG AAATCGAAGC CAGCCCACAG GATCCCATCC ATCTGGAAGT GTACTCCCCG 
CCTTCCTCCT CGGGCCTGGC AGGACAAGAT GGAGAGTCCA CATCCCTGTC ACTCAGCAGC 
CACAGAGGAG GAAGTCCTCC GGCCCCTTCA CAGGCAGCCA CAGACACACA AGCTGGAGCC 
AGTCCCAACT CCCCTGGAGC AGACTTTGGA AACACTAGAG GGGCCAGCCC TACTGAGCTC 
AGCAAAGCTG AGCACCCCGG ATCCAGCCCA CAGGCCTCTG TGGACTGGAA TGTGGTGGCA 
GATATCACTA TTTCTGAGCC TGCCACTGAG CCCATGGGAG CCAGAGGCGT CTCGGAGTCT 
GAGTCTGGGG TCTTCAACAC AGCAGAGAGT CCTACTTCCA GCTTGCAGGC CTCCATGGAC 
AAGACGCAGG AAATATGGGC CTCGCTGCAC AGGGAAGGGC TGGACCCCCA CTCCCCATCT 
GCACCCTTGG GGGTCCCTGA AGTCCCCTTG ATGCCCAAGT TCACCCCAGG TTTGGGGCCT 
TGGGCTGCTA CAGATGGAGG AGTCACAGTG GATCCTATGG CTTCCACAGT CACACTGGAC 
ACCAGCGATG CTGGTGGGCA TTGGGAACCT GGATCCCACA TGGTTGAGGG AGCAGAAAGC 
CCCACCTTGA GCCCTCAAAT GGCTGTGGAC ACAAGTGTGG TGACATCCGT CACGTCCTTG 
GACCTGGGGG ACAAGGTCAG GGTCCTGGCC GTGTTCACAG TGGCCTCCTC AAGCTCCCAG 
CCCCACCCAG AGCCAGAGAG CCAGCTGGTG ACCCAGGGAA CCCTGGGAGC CTTGGAGCCT 
CCACAGGAGG GCAGCCACTC AGGGGACCCC ATTCTTCCTC CTTGGACGCC GACAGCAGCC 
AACAAGGATG AGCCCATTTC AGTTTCCTCG GGGGAGCCTA CAGTGCCTTG GGACTCCCCC 
AGCACCCTGC TGCCTGCCTC TCTGGGCCCA GAGGAATTTG AGCTGGAGGT CCTGATGGGG 
AGCCCCAGTG TGGAGAGCTT CTGGGAGGAG GCAGCAAGTG GAGAGGAGCC TGCCCTGCCA 
GGGACTCCTG CAAATGGGAG TGCAGAGGAG GGCCCCTTGG ATCCCTGTGA GAACAACCCT 
TGCCTGCACG GAGGCACCTG TAGAGCCAAT GGCACCATGT ACGGCTGCAG CTGTGACCAG 
GGGTTCGCTG GAGAGAACTG TGAGATCGAT ATTGACGATT GCATCTCCAG CCCCTGTGAG 
AACGGTGGCA CCTGCATCGA CGAGGTCAAC GCCTTCATCT GCCTTTGCCT CCCCAGCTAT 
GGGGGAAGCC TCTGCGAGAA AGATACAGAG GGCTGTGACC ACGGCTGGCA CAAGTTTCAG 
GGCCACTGCT ACCGTTACTT TGCCCACCGG CGGGCATGGG AGGACGCCGA GAGGGACTGC 
CGCCGCCGAG CCGGCCACCT AACCAGCATC CACTCATCAG AAGAACACAA CTTCATTAAC 
AGTTTTGGGC GTGAAAACAC ATGGATCGGC TTGAACGACA GGATCGTGGA AAGGGATTTC 
CAGTGGACGG ACAACACAGG GCTGCAATTT GAGAACTGGC GGGAAAATCA GCCGGACAAC 
TTCTTCGCGG GCGGGGAGGA CTGTGTGGTG ATGGTTGCAC ATGAGAGCGG GCGCTGGAAC 
GACGTTCCCT GCAACTACAA CCTCCCCTAC GTCTGTAAGA AGGGCACAGT GCTGTGTGGT 
CCCCCTCCAG CGGTGGAGAA TGCCTCGCCC ATTGGTGCCC GCAAGGCCAA GTATAATGTC 
CATGCCACAG TACGCTACCA GTGTGATGAA GGATTTGCCC AGCACCATGT GGCCATCATC 
CGGTGCCGGA GCAATGGCAA ATGGGACCGG CCCCAGATCA TCTGCACCAA ACCCAGAAGG 
TCCCATAGGA TGCGGCGACA CCACCATCAC CACCAGCATC ACCACCAGCA TCGCCACCAC 
AAATCTCGCA AGGAGCGCAG AAAACACAAG AAGCACCCAG CAGAGGACTG GGAAAAGGAA 
GAAGGGAATT TCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCAN ( XM_015471872.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327358.1 Bos taurus neurocan (NCAN), transcript variant X1, mRNA.

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CloneID	OBa20801
Clone ID Related Accession (Same CDS sequence)	XM_015471872.2 , XM_015471872.1
Accession Version	XM_015471872.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurocan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104001.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCAT GCTCTGACCA TCAGACTTCA CTTGCTGGGG TTCTGAAGTC CAGGATGGGG 
GCCTTGTCTG TCTGGGTCTC AGGCCTCCTG ACTCTCCAGA TGCTTCTCTT TGTGGCTGGG 
GAGCAGGGCC CACAGCACAC CACCGCAGCT GCAGCCACTG CTGAGAAGGG GCTCCACATG 
CAGAAAGTGG GGTCTGGGTC AGTCCGGGCT GCCCTGGCAG AGTTGGTGGC CCTGCCCTGT 
CTCTTTAGCC TGCGGCCACG GCCAGGTGCA GCTCGAGAAG CCCCACGGAT CAAATGGACC 
AAGGTGCGGA CTGCATCAGG CCAGCGGCAG GACTTGCCCA TCCTAGTAGC CAAGGACAAC 
GTGGTCCGAG TCGCCAAGGG CTGGCAGGGA CGTGTGTCAC TTCCCGCCTA CCCCCGGCAC 
CGGGTCAACG CCACGTTGCT GCTGGGACCA CTGAGGGCCA GCGACTCCGG GCTCTACCGC 
TGCCAGGTGG TGAGGGGCAT CGAGGATGAG CAGGACCTGG TGCCCCTGGA GGTGACGGGT 
GTTGTGTTCC ACTACCGAGC AGCCCTGGAT CGCTACTCTC TAACTTTTGC TGAGGCTCAG 
GAGGCCTGTC TTCTCAACTC AGCCACCATT GCAGCCCCGC GGCACCTGCA GGCTGCCTTT 
GAGGACGGCT TTGACAACTG CGATGCCGGG TGGCTTTCTG ACCGCACTGT TCGGTATCCT 
ATCACCCAGT CCCGTCCTGG TTGCTATGGT GACCGTAGCA GCCTTCCGGG GGTGAGGAAC 
TATGGGAGGC GAGACCCACG GGAACTCTAC GATGTGTATT GCTTTGCCCG TGAGCTGGGG 
GGCGAGGTCT TCTACGTAGG CCTGGCCCGC CGCCTGACAC TGGCCGGCGC ACGTGCCCAG 
TGCCGACGCC AGGGCGCCGT GCTGGCCTCG GTAGGACAGC TGCACCTGGC CTGGCATGAG 
GGCCTGGACC AGTGCGACCC GGGCTGGCTG GCCGACGGCA GCGTGCGCTA CCCGATCCAG 
ACGCCGCGTC GGCGCTGCGG TGGCCCAGCC CCTGGCGTGC GCACCGTATA CCGCTTTGCC 
AACCGCACCG GCTTCCCGGC ACCTGGAGAG CGCTTCGATG CCTACTGCTT CAGAGCTCAT 
CACCCCACAT CCCAACATGG AGACCCAGAG ACCCCCTCAT CTGGAGATGA GGGGGAGATT 
CTGTCGGCAG AAGGGCCCCC CGCTGGAGAA TTGGAGCCCA GTCTGCAGGA GGAGGAGGAG 
GTAGTCACCC CGGACATCCA GGAGCCTCTG GTGTCTAGTG GGGAAGAAGA GTCTCTGATC 
TTGGCAGAGA AGCAGGAATC TCAAGCGACC CCCAGCCGTG CCCCTGGGGT CCCCACATTG 
GCCTCAAGTC CTGCCCTGGA GGCTGAGGAG GTGTGGCTGA GCACAGTGGC CCCCAGCCAC 
AGCAGCGTGG AGGCAGGCAC TGTGGTGGGC ACGCACACAG ACACAGCCCC AGCAAGCACC 
ACGCCCAGGA AGAGGGAGCG CTTTAAAGGG CTGAACGGGC GCCACTTCCA GCAACAGGAG 
CCCCAGCAAG GGCTGAAGGG GGCACTCGAG GCCAGCATCC AGCCCCCCAC ACCAGAGGCT 
GCTGAGAATT ATGTGGAGCC TCCCATGACC ACTGGAACCA CAGACGCCTT GGGGAGCGGC 
CAGAGCCAAA GCCCCTGGGC TGTGCTGACA AATGAGGTGG AAATGCCCAG AGCTGGTTCC 
CTTGGTGGCA GGAGCCCCCA AGAGCCCTGG CTGTGGCTCC CAACCGTGGT CCCACCCATC 
ATCCCAGACC CCAGCAGAGC CCTTGGCCTG GAACTAGGAG AAGCCAAGCG CTTTAGTATG 
AGACCAGTCA CTTCCGACCT GCCCTGGTCC CCCTCAGAGA CCACTCCCAA CCCTTCCAAT 
GGCCCCAGCC CTACCCCCTG GAAGGCTTCT CCTGTGGCCA TTTCTCCAGA CCTCCCTGTC 
ATGGCCATGC TTCGCGCCCC CAAACTGTGG CTCCTATCAC AGGAGCCTCC TACATCCCTC 
CCCACTGAGG CCAGTGGAGT CAAGGGGCAT AGTGAGGCCA TGGCCACTGC CCTGCCCTCC 
CCTGCCTCAG AAATCGAAGC CAGCCCACAG GATCCCATCC ATCTGGAAGT GTACTCCCCG 
CCTTCCTCCT CGGGCCTGGC AGGACAAGAT GGAGAGTCCA CATCCCTGTC ACTCAGCAGC 
CACAGAGGAG GAAGTCCTCC GGCCCCTTCA CAGGCAGCCA CAGACACACA AGCTGGAGCC 
AGTCCCAACT CCCCTGGAGC AGACTTTGGA AACACTAGAG GGGCCAGCCC TACTGAGCTC 
AGCAAAGCTG AGCACCCCGG ATCCAGCCCA CAGGCCTCTG TGGACTGGAA TGTGGTGGCA 
GATATCACTA TTTCTGAGCC TGCCACTGAG CCCATGGGAG CCAGAGGCGT CTCGGAGTCT 
GAGTCTGGGG TCTTCAACAC AGCAGAGAGT CCTACTTCCA GCTTGCAGGC CTCCATGGAC 
AAGACGCAGG AAATATGGGC CTCGCTGCAC AGGGAAGGGC TGGACCCCCA CTCCCCATCT 
GCACCCTTGG GGGTCCCTGA AGTCCCCTTG ATGCCCAAGT TCACCCCAGG TTTGGGGCCT 
TGGGCTGCTA CAGATGGAGG AGTCACAGTG GATCCTATGG CTTCCACAGT CACACTGGAC 
ACCAGCGATG CTGGTGGGCA TTGGGAACCT GGATCCCACA TGGTTGAGGG AGCAGAAAGC 
CCCACCTTGA GCCCTCAAAT GGCTGTGGAC ACAAGTGTGG TGACATCCGT CACGTCCTTG 
GACCTGGGGG ACAAGGTCAG GGTCCTGGCC GTGTTCACAG TGGCCTCCTC AAGCTCCCAG 
CCCCACCCAG AGCCAGAGAG CCAGCTGGTG ACCCAGGGAA CCCTGGGAGC CTTGGAGCCT 
CCACAGGAGG GCAGCCACTC AGGGGACCCC ATTCTTCCTC CTTGGACGCC GACAGCAGCC 
AACAAGGATG AGCCCATTTC AGTTTCCTCG GGGGAGCCTA CAGTGCCTTG GGACTCCCCC 
AGCACCCTGC TGCCTGCCTC TCTGGGCCCA GAGGAATTTG AGCTGGAGGT CCTGATGGGG 
AGCCCCAGTG TGGAGAGCTT CTGGGAGGAG GCAGCAAGTG GAGAGGAGCC TGCCCTGCCA 
GGGACTCCTG CAAATGGGAG TGCAGAGGAG GGCCCCTTGG ATCCCTGTGA GAACAACCCT 
TGCCTGCACG GAGGCACCTG TAGAGCCAAT GGCACCATGT ACGGCTGCAG CTGTGACCAG 
GGGTTCGCTG GAGAGAACTG TGAGATCGAT ATTGACGATT GCATCTCCAG CCCCTGTGAG 
AACGGTGGCA CCTGCATCGA CGAGGTCAAC GCCTTCATCT GCCTTTGCCT CCCCAGCTAT 
GGGGGAAGCC TCTGCGAGAA AGATACAGAG GGCTGTGACC ACGGCTGGCA CAAGTTTCAG 
GGCCACTGCT ACCGTTACTT TGCCCACCGG CGGGCATGGG AGGACGCCGA GAGGGACTGC 
CGCCGCCGAG CCGGCCACCT AACCAGCATC CACTCATCAG AAGAACACAA CTTCATTAAC 
AGTTTTGGGC GTGAAAACAC ATGGATCGGC TTGAACGACA GGATCGTGGA AAGGGATTTC 
CAGTGGACGG ACAACACAGG GCTGCAATTT GAGAACTGGC GGGAAAATCA GCCGGACAAC 
TTCTTCGCGG GCGGGGAGGA CTGTGTGGTG ATGGTTGCAC ATGAGAGCGG GCGCTGGAAC 
GACGTTCCCT GCAACTACAA CCTCCCCTAC GTCTGTAAGA AGGGCACAGT GCTGTGTGGT 
CCCCCTCCAG CGGTGGAGAA TGCCTCGCCC ATTGGTGCCC GCAAGGCCAA GTATAATGTC 
CATGCCACAG TACGCTACCA GTGTGATGAA GGATTTGCCC AGCACCATGT GGCCATCATC 
CGGTGCCGGA GCAATGGCAA ATGGGACCGG CCCCAGATCA TCTGCACCAA ACCCAGAAGG 
TCCCATAGGA TGCGGCGACA CCACCATCAC CACCAGCATC ACCACCAGCA TCGCCACCAC 
AAATCTCGCA AGGAGCGCAG AAAACACAAG AAGCACCCAG CAGAGGACTG GGAAAAGGAA 
GAAGGGAATT TCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327358.1
CDS	662..4819
Translation	MHSCSDHQTSLAGVLKSRMGALSVWVSGLLTLQMLLFVAGEQGPQHTTAAAATAEKGLHMQKVGSGSVRAALAELVALPCLFSLRPRPGAAREAPRIKWTKVRTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRVNATLLLGPLRASDSGLYRCQVVRGIEDEQDLVPLEVTGVVFHYRAALDRYSLTFAEAQEACLLNSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRNYGRRDPRELYDVYCFARELGGEVFYVGLARRLTLAGARAQCRRQGAVLASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGERFDAYCFRAHHPTSQHGDPETPSSGDEGEILSAEGPPAGELEPSLQEEEEVVTPDIQEPLVSSGEEESLILAEKQESQATPSRAPGVPTLASSPALEAEEVWLSTVAPSHSSVEAGTVVGTHTDTAPASTTPRKRERFKGLNGRHFQQQEPQQGLKGALEASIQPPTPEAAENYVEPPMTTGTTDALGSGQSQSPWAVLTNEVEMPRAGSLGGRSPQEPWLWLPTVVPPIIPDPSRALGLELGEAKRFSMRPVTSDLPWSPSETTPNPSNGPSPTPWKASPVAISPDLPVMAMLRAPKLWLLSQEPPTSLPTEASGVKGHSEAMATALPSPASEIEASPQDPIHLEVYSPPSSSGLAGQDGESTSLSLSSHRGGSPPAPSQAATDTQAGASPNSPGADFGNTRGASPTELSKAEHPGSSPQASVDWNVVADITISEPATEPMGARGVSESESGVFNTAESPTSSLQASMDKTQEIWASLHREGLDPHSPSAPLGVPEVPLMPKFTPGLGPWAATDGGVTVDPMASTVTLDTSDAGGHWEPGSHMVEGAESPTLSPQMAVDTSVVTSVTSLDLGDKVRVLAVFTVASSSSQPHPEPESQLVTQGTLGALEPPQEGSHSGDPILPPWTPTAANKDEPISVSSGEPTVPWDSPSTLLPASLGPEEFELEVLMGSPSVESFWEEAASGEEPALPGTPANGSAEEGPLDPCENNPCLHGGTCRANGTMYGCSCDQGFAGENCEIDIDDCISSPCENGGTCIDEVNAFICLCLPSYGGSLCEKDTEGCDHGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSIHSSEEHNFINSFGRENTWIGLNDRIVERDFQWTDNTGLQFENWRENQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASPIGARKAKYNVHATVRYQCDEGFAQHHVAIIRCRSNGKWDRPQIICTKPRRSHRMRRHHHHHQHHHQHRHHKSRKERRKHKKHPAEDWEKEEGNFC

Target ORF information:

RefSeq Version	XM_015471872.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurocan (NCAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471872.1

ORF Insert Sequence:

ATGCACTCAT GCTCTGACCA TCAGACTTCA CTTGCTGGGG TTCTGAAGTC CAGGATGGGG 
GCCTTGTCTG TCTGGGTCTC AGGCCTCCTG ACTCTCCAGA TGCTTCTCTT TGTGGCTGGG 
GAGCAGGGCC CACAGCACAC CACCGCAGCT GCAGCCACTG CTGAGAAGGG GCTCCACATG 
CAGAAAGTGG GGTCTGGGTC AGTCCGGGCT GCCCTGGCAG AGTTGGTGGC CCTGCCCTGT 
CTCTTTAGCC TGCGGCCACG GCCAGGTGCA GCTCGAGAAG CCCCACGGAT CAAATGGACC 
AAGGTGCGGA CTGCATCAGG CCAGCGGCAG GACTTGCCCA TCCTAGTAGC CAAGGACAAC 
GTGGTCCGAG TCGCCAAGGG CTGGCAGGGA CGTGTGTCAC TTCCCGCCTA CCCCCGGCAC 
CGGGTCAACG CCACGTTGCT GCTGGGACCA CTGAGGGCCA GCGACTCCGG GCTCTACCGC 
TGCCAGGTGG TGAGGGGCAT CGAGGATGAG CAGGACCTGG TGCCCCTGGA GGTGACGGGT 
GTTGTGTTCC ACTACCGAGC AGCCCTGGAT CGCTACTCTC TAACTTTTGC TGAGGCTCAG 
GAGGCCTGTC TTCTCAACTC AGCCACCATT GCAGCCCCGC GGCACCTGCA GGCTGCCTTT 
GAGGACGGCT TTGACAACTG CGATGCCGGG TGGCTTTCTG ACCGCACTGT TCGGTATCCT 
ATCACCCAGT CCCGTCCTGG TTGCTATGGT GACCGTAGCA GCCTTCCGGG GGTGAGGAAC 
TATGGGAGGC GAGACCCACG GGAACTCTAC GATGTGTATT GCTTTGCCCG TGAGCTGGGG 
GGCGAGGTCT TCTACGTAGG CCTGGCCCGC CGCCTGACAC TGGCCGGCGC ACGTGCCCAG 
TGCCGACGCC AGGGCGCCGT GCTGGCCTCG GTAGGACAGC TGCACCTGGC CTGGCATGAG 
GGCCTGGACC AGTGCGACCC GGGCTGGCTG GCCGACGGCA GCGTGCGCTA CCCGATCCAG 
ACGCCGCGTC GGCGCTGCGG TGGCCCAGCC CCTGGCGTGC GCACCGTATA CCGCTTTGCC 
AACCGCACCG GCTTCCCGGC ACCTGGAGAG CGCTTCGATG CCTACTGCTT CAGAGCTCAT 
CACCCCACAT CCCAACATGG AGACCCAGAG ACCCCCTCAT CTGGAGATGA GGGGGAGATT 
CTGTCGGCAG AAGGGCCCCC CGCTGGAGAA TTGGAGCCCA GTCTGCAGGA GGAGGAGGAG 
GTAGTCACCC CGGACATCCA GGAGCCTCTG GTGTCTAGTG GGGAAGAAGA GTCTCTGATC 
TTGGCAGAGA AGCAGGAATC TCAAGCGACC CCCAGCCGTG CCCCTGGGGT CCCCACATTG 
GCCTCAAGTC CTGCCCTGGA GGCTGAGGAG GTGTGGCTGA GCACAGTGGC CCCCAGCCAC 
AGCAGCGTGG AGGCAGGCAC TGTGGTGGGC ACGCACACAG ACACAGCCCC AGCAAGCACC 
ACGCCCAGGA AGAGGGAGCG CTTTAAAGGG CTGAACGGGC GCCACTTCCA GCAACAGGAG 
CCCCAGCAAG GGCTGAAGGG GGCACTCGAG GCCAGCATCC AGCCCCCCAC ACCAGAGGCT 
GCTGAGAATT ATGTGGAGCC TCCCATGACC ACTGGAACCA CAGACGCCTT GGGGAGCGGC 
CAGAGCCAAA GCCCCTGGGC TGTGCTGACA AATGAGGTGG AAATGCCCAG AGCTGGTTCC 
CTTGGTGGCA GGAGCCCCCA AGAGCCCTGG CTGTGGCTCC CAACCGTGGT CCCACCCATC 
ATCCCAGACC CCAGCAGAGC CCTTGGCCTG GAACTAGGAG AAGCCAAGCG CTTTAGTATG 
AGACCAGTCA CTTCCGACCT GCCCTGGTCC CCCTCAGAGA CCACTCCCAA CCCTTCCAAT 
GGCCCCAGCC CTACCCCCTG GAAGGCTTCT CCTGTGGCCA TTTCTCCAGA CCTCCCTGTC 
ATGGCCATGC TTCGCGCCCC CAAACTGTGG CTCCTATCAC AGGAGCCTCC TACATCCCTC 
CCCACTGAGG CCAGTGGAGT CAAGGGGCAT AGTGAGGCCA TGGCCACTGC CCTGCCCTCC 
CCTGCCTCAG AAATCGAAGC CAGCCCACAG GATCCCATCC ATCTGGAAGT GTACTCCCCG 
CCTTCCTCCT CGGGCCTGGC AGGACAAGAT GGAGAGTCCA CATCCCTGTC ACTCAGCAGC 
CACAGAGGAG GAAGTCCTCC GGCCCCTTCA CAGGCAGCCA CAGACACACA AGCTGGAGCC 
AGTCCCAACT CCCCTGGAGC AGACTTTGGA AACACTAGAG GGGCCAGCCC TACTGAGCTC 
AGCAAAGCTG AGCACCCCGG ATCCAGCCCA CAGGCCTCTG TGGACTGGAA TGTGGTGGCA 
GATATCACTA TTTCTGAGCC TGCCACTGAG CCCATGGGAG CCAGAGGCGT CTCGGAGTCT 
GAGTCTGGGG TCTTCAACAC AGCAGAGAGT CCTACTTCCA GCTTGCAGGC CTCCATGGAC 
AAGACGCAGG AAATATGGGC CTCGCTGCAC AGGGAAGGGC TGGACCCCCA CTCCCCATCT 
GCACCCTTGG GGGTCCCTGA AGTCCCCTTG ATGCCCAAGT TCACCCCAGG TTTGGGGCCT 
TGGGCTGCTA CAGATGGAGG AGTCACAGTG GATCCTATGG CTTCCACAGT CACACTGGAC 
ACCAGCGATG CTGGTGGGCA TTGGGAACCT GGATCCCACA TGGTTGAGGG AGCAGAAAGC 
CCCACCTTGA GCCCTCAAAT GGCTGTGGAC ACAAGTGTGG TGACATCCGT CACGTCCTTG 
GACCTGGGGG ACAAGGTCAG GGTCCTGGCC GTGTTCACAG TGGCCTCCTC AAGCTCCCAG 
CCCCACCCAG AGCCAGAGAG CCAGCTGGTG ACCCAGGGAA CCCTGGGAGC CTTGGAGCCT 
CCACAGGAGG GCAGCCACTC AGGGGACCCC ATTCTTCCTC CTTGGACGCC GACAGCAGCC 
AACAAGGATG AGCCCATTTC AGTTTCCTCG GGGGAGCCTA CAGTGCCTTG GGACTCCCCC 
AGCACCCTGC TGCCTGCCTC TCTGGGCCCA GAGGAATTTG AGCTGGAGGT CCTGATGGGG 
AGCCCCAGTG TGGAGAGCTT CTGGGAGGAG GCAGCAAGTG GAGAGGAGCC TGCCCTGCCA 
GGGACTCCTG CAAATGGGAG TGCAGAGGAG GGCCCCTTGG ATCCCTGTGA GAACAACCCT 
TGCCTGCACG GAGGCACCTG TAGAGCCAAT GGCACCATGT ACGGCTGCAG CTGTGACCAG 
GGGTTCGCTG GAGAGAACTG TGAGATCGAT ATTGACGATT GCATCTCCAG CCCCTGTGAG 
AACGGTGGCA CCTGCATCGA CGAGGTCAAC GCCTTCATCT GCCTTTGCCT CCCCAGCTAT 
GGGGGAAGCC TCTGCGAGAA AGATACAGAG GGCTGTGACC ACGGCTGGCA CAAGTTTCAG 
GGCCACTGCT ACCGTTACTT TGCCCACCGG CGGGCATGGG AGGACGCCGA GAGGGACTGC 
CGCCGCCGAG CCGGCCACCT AACCAGCATC CACTCATCAG AAGAACACAA CTTCATTAAC 
AGTTTTGGGC GTGAAAACAC ATGGATCGGC TTGAACGACA GGATCGTGGA AAGGGATTTC 
CAGTGGACGG ACAACACAGG GCTGCAATTT GAGAACTGGC GGGAAAATCA GCCGGACAAC 
TTCTTCGCGG GCGGGGAGGA CTGTGTGGTG ATGGTTGCAC ATGAGAGCGG GCGCTGGAAC 
GACGTTCCCT GCAACTACAA CCTCCCCTAC GTCTGTAAGA AGGGCACAGT GCTGTGTGGT 
CCCCCTCCAG CGGTGGAGAA TGCCTCGCCC ATTGGTGCCC GCAAGGCCAA GTATAATGTC 
CATGCCACAG TACGCTACCA GTGTGATGAA GGATTTGCCC AGCACCATGT GGCCATCATC 
CGGTGCCGGA GCAATGGCAA ATGGGACCGG CCCCAGATCA TCTGCACCAA ACCCAGAAGG 
TCCCATAGGA TGCGGCGACA CCACCATCAC CACCAGCATC ACCACCAGCA TCGCCACCAC 
AAATCTCGCA AGGAGCGCAG AAAACACAAG AAGCACCCAG CAGAGGACTG GGAAAAGGAA 
GAAGGGAATT TCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCAN ( XM_005208460.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208517.1 Bos taurus neurocan (NCAN), transcript variant X2, mRNA.

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CloneID	OBa20802
Clone ID Related Accession (Same CDS sequence)	XM_005208460.3 , XM_005208460.4
Accession Version	XM_005208460.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurocan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104001.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCT TGTCTGTCTG GGTCTCAGGC CTCCTGACTC TCCAGATGCT TCTCTTTGTG 
GCTGGGGAGC AGGGCCCACA GCACACCACC GCAGCTGCAG CCACTGCTGA GAAGGGGCTC 
CACATGCAGA AAGTGGGGTC TGGGTCAGTC CGGGCTGCCC TGGCAGAGTT GGTGGCCCTG 
CCCTGTCTCT TTAGCCTGCG GCCACGGCCA GGTGCAGCTC GAGAAGCCCC ACGGATCAAA 
TGGACCAAGG TGCGGACTGC ATCAGGCCAG CGGCAGGACT TGCCCATCCT AGTAGCCAAG 
GACAACGTGG TCCGAGTCGC CAAGGGCTGG CAGGGACGTG TGTCACTTCC CGCCTACCCC 
CGGCACCGGG TCAACGCCAC GTTGCTGCTG GGACCACTGA GGGCCAGCGA CTCCGGGCTC 
TACCGCTGCC AGGTGGTGAG GGGCATCGAG GATGAGCAGG ACCTGGTGCC CCTGGAGGTG 
ACGGGTGTTG TGTTCCACTA CCGAGCAGCC CTGGATCGCT ACTCTCTAAC TTTTGCTGAG 
GCTCAGGAGG CCTGTCTTCT CAACTCAGCC ACCATTGCAG CCCCGCGGCA CCTGCAGGCT 
GCCTTTGAGG ACGGCTTTGA CAACTGCGAT GCCGGGTGGC TTTCTGACCG CACTGTTCGG 
TATCCTATCA CCCAGTCCCG TCCTGGTTGC TATGGTGACC GTAGCAGCCT TCCGGGGGTG 
AGGAACTATG GGAGGCGAGA CCCACGGGAA CTCTACGATG TGTATTGCTT TGCCCGTGAG 
CTGGGGGGCG AGGTCTTCTA CGTAGGCCTG GCCCGCCGCC TGACACTGGC CGGCGCACGT 
GCCCAGTGCC GACGCCAGGG CGCCGTGCTG GCCTCGGTAG GACAGCTGCA CCTGGCCTGG 
CATGAGGGCC TGGACCAGTG CGACCCGGGC TGGCTGGCCG ACGGCAGCGT GCGCTACCCG 
ATCCAGACGC CGCGTCGGCG CTGCGGTGGC CCAGCCCCTG GCGTGCGCAC CGTATACCGC 
TTTGCCAACC GCACCGGCTT CCCGGCACCT GGAGAGCGCT TCGATGCCTA CTGCTTCAGA 
GCTCATCACC CCACATCCCA ACATGGAGAC CCAGAGACCC CCTCATCTGG AGATGAGGGG 
GAGATTCTGT CGGCAGAAGG GCCCCCCGCT GGAGAATTGG AGCCCAGTCT GCAGGAGGAG 
GAGGAGGTAG TCACCCCGGA CATCCAGGAG CCTCTGGTGT CTAGTGGGGA AGAAGAGTCT 
CTGATCTTGG CAGAGAAGCA GGAATCTCAA GCGACCCCCA GCCGTGCCCC TGGGGTCCCC 
ACATTGGCCT CAAGTCCTGC CCTGGAGGCT GAGGAGGTGT GGCTGAGCAC AGTGGCCCCC 
AGCCACAGCA GCGTGGAGGC AGGCACTGTG GTGGGCACGC ACACAGACAC AGCCCCAGCA 
AGCACCACGC CCAGGAAGAG GGAGCGCTTT AAAGGGCTGA ACGGGCGCCA CTTCCAGCAA 
CAGGAGCCCC AGCAAGGGCT GAAGGGGGCA CTCGAGGCCA GCATCCAGCC CCCCACACCA 
GAGGCTGCTG AGAATTATGT GGAGCCTCCC ATGACCACTG GAACCACAGA CGCCTTGGGG 
AGCGGCCAGA GCCAAAGCCC CTGGGCTGTG CTGACAAATG AGGTGGAAAT GCCCAGAGCT 
GGTTCCCTTG GTGGCAGGAG CCCCCAAGAG CCCTGGCTGT GGCTCCCAAC CGTGGTCCCA 
CCCATCATCC CAGACCCCAG CAGAGCCCTT GGCCTGGAAC TAGGAGAAGC CAAGCGCTTT 
AGTATGAGAC CAGTCACTTC CGACCTGCCC TGGTCCCCCT CAGAGACCAC TCCCAACCCT 
TCCAATGGCC CCAGCCCTAC CCCCTGGAAG GCTTCTCCTG TGGCCATTTC TCCAGACCTC 
CCTGTCATGG CCATGCTTCG CGCCCCCAAA CTGTGGCTCC TATCACAGGA GCCTCCTACA 
TCCCTCCCCA CTGAGGCCAG TGGAGTCAAG GGGCATAGTG AGGCCATGGC CACTGCCCTG 
CCCTCCCCTG CCTCAGAAAT CGAAGCCAGC CCACAGGATC CCATCCATCT GGAAGTGTAC 
TCCCCGCCTT CCTCCTCGGG CCTGGCAGGA CAAGATGGAG AGTCCACATC CCTGTCACTC 
AGCAGCCACA GAGGAGGAAG TCCTCCGGCC CCTTCACAGG CAGCCACAGA CACACAAGCT 
GGAGCCAGTC CCAACTCCCC TGGAGCAGAC TTTGGAAACA CTAGAGGGGC CAGCCCTACT 
GAGCTCAGCA AAGCTGAGCA CCCCGGATCC AGCCCACAGG CCTCTGTGGA CTGGAATGTG 
GTGGCAGATA TCACTATTTC TGAGCCTGCC ACTGAGCCCA TGGGAGCCAG AGGCGTCTCG 
GAGTCTGAGT CTGGGGTCTT CAACACAGCA GAGAGTCCTA CTTCCAGCTT GCAGGCCTCC 
ATGGACAAGA CGCAGGAAAT ATGGGCCTCG CTGCACAGGG AAGGGCTGGA CCCCCACTCC 
CCATCTGCAC CCTTGGGGGT CCCTGAAGTC CCCTTGATGC CCAAGTTCAC CCCAGGTTTG 
GGGCCTTGGG CTGCTACAGA TGGAGGAGTC ACAGTGGATC CTATGGCTTC CACAGTCACA 
CTGGACACCA GCGATGCTGG TGGGCATTGG GAACCTGGAT CCCACATGGT TGAGGGAGCA 
GAAAGCCCCA CCTTGAGCCC TCAAATGGCT GTGGACACAA GTGTGGTGAC ATCCGTCACG 
TCCTTGGACC TGGGGGACAA GGTCAGGGTC CTGGCCGTGT TCACAGTGGC CTCCTCAAGC 
TCCCAGCCCC ACCCAGAGCC AGAGAGCCAG CTGGTGACCC AGGGAACCCT GGGAGCCTTG 
GAGCCTCCAC AGGAGGGCAG CCACTCAGGG GACCCCATTC TTCCTCCTTG GACGCCGACA 
GCAGCCAACA AGGATGAGCC CATTTCAGTT TCCTCGGGGG AGCCTACAGT GCCTTGGGAC 
TCCCCCAGCA CCCTGCTGCC TGCCTCTCTG GGCCCAGAGG AATTTGAGCT GGAGGTCCTG 
ATGGGGAGCC CCAGTGTGGA GAGCTTCTGG GAGGAGGCAG CAAGTGGAGA GGAGCCTGCC 
CTGCCAGGGA CTCCTGCAAA TGGGAGTGCA GAGGAGGGCC CCTTGGATCC CTGTGAGAAC 
AACCCTTGCC TGCACGGAGG CACCTGTAGA GCCAATGGCA CCATGTACGG CTGCAGCTGT 
GACCAGGGGT TCGCTGGAGA GAACTGTGAG ATCGATATTG ACGATTGCAT CTCCAGCCCC 
TGTGAGAACG GTGGCACCTG CATCGACGAG GTCAACGCCT TCATCTGCCT TTGCCTCCCC 
AGCTATGGGG GAAGCCTCTG CGAGAAAGAT ACAGAGGGCT GTGACCACGG CTGGCACAAG 
TTTCAGGGCC ACTGCTACCG TTACTTTGCC CACCGGCGGG CATGGGAGGA CGCCGAGAGG 
GACTGCCGCC GCCGAGCCGG CCACCTAACC AGCATCCACT CATCAGAAGA ACACAACTTC 
ATTAACAGTT TTGGGCGTGA AAACACATGG ATCGGCTTGA ACGACAGGAT CGTGGAAAGG 
GATTTCCAGT GGACGGACAA CACAGGGCTG CAATTTGAGA ACTGGCGGGA AAATCAGCCG 
GACAACTTCT TCGCGGGCGG GGAGGACTGT GTGGTGATGG TTGCACATGA GAGCGGGCGC 
TGGAACGACG TTCCCTGCAA CTACAACCTC CCCTACGTCT GTAAGAAGGG CACAGTGCTG 
TGTGGTCCCC CTCCAGCGGT GGAGAATGCC TCGCCCATTG GTGCCCGCAA GGCCAAGTAT 
AATGTCCATG CCACAGTACG CTACCAGTGT GATGAAGGAT TTGCCCAGCA CCATGTGGCC 
ATCATCCGGT GCCGGAGCAA TGGCAAATGG GACCGGCCCC AGATCATCTG CACCAAACCC 
AGAAGGTCCC ATAGGATGCG GCGACACCAC CATCACCACC AGCATCACCA CCAGCATCGC 
CACCACAAAT CTCGCAAGGA GCGCAGAAAA CACAAGAAGC ACCCAGCAGA GGACTGGGAA 
AAGGAAGAAG GGAATTTCTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208517.1
CDS	108..4211
Translation	MGALSVWVSGLLTLQMLLFVAGEQGPQHTTAAAATAEKGLHMQKVGSGSVRAALAELVALPCLFSLRPRPGAAREAPRIKWTKVRTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRVNATLLLGPLRASDSGLYRCQVVRGIEDEQDLVPLEVTGVVFHYRAALDRYSLTFAEAQEACLLNSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRNYGRRDPRELYDVYCFARELGGEVFYVGLARRLTLAGARAQCRRQGAVLASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGERFDAYCFRAHHPTSQHGDPETPSSGDEGEILSAEGPPAGELEPSLQEEEEVVTPDIQEPLVSSGEEESLILAEKQESQATPSRAPGVPTLASSPALEAEEVWLSTVAPSHSSVEAGTVVGTHTDTAPASTTPRKRERFKGLNGRHFQQQEPQQGLKGALEASIQPPTPEAAENYVEPPMTTGTTDALGSGQSQSPWAVLTNEVEMPRAGSLGGRSPQEPWLWLPTVVPPIIPDPSRALGLELGEAKRFSMRPVTSDLPWSPSETTPNPSNGPSPTPWKASPVAISPDLPVMAMLRAPKLWLLSQEPPTSLPTEASGVKGHSEAMATALPSPASEIEASPQDPIHLEVYSPPSSSGLAGQDGESTSLSLSSHRGGSPPAPSQAATDTQAGASPNSPGADFGNTRGASPTELSKAEHPGSSPQASVDWNVVADITISEPATEPMGARGVSESESGVFNTAESPTSSLQASMDKTQEIWASLHREGLDPHSPSAPLGVPEVPLMPKFTPGLGPWAATDGGVTVDPMASTVTLDTSDAGGHWEPGSHMVEGAESPTLSPQMAVDTSVVTSVTSLDLGDKVRVLAVFTVASSSSQPHPEPESQLVTQGTLGALEPPQEGSHSGDPILPPWTPTAANKDEPISVSSGEPTVPWDSPSTLLPASLGPEEFELEVLMGSPSVESFWEEAASGEEPALPGTPANGSAEEGPLDPCENNPCLHGGTCRANGTMYGCSCDQGFAGENCEIDIDDCISSPCENGGTCIDEVNAFICLCLPSYGGSLCEKDTEGCDHGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSIHSSEEHNFINSFGRENTWIGLNDRIVERDFQWTDNTGLQFENWRENQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASPIGARKAKYNVHATVRYQCDEGFAQHHVAIIRCRSNGKWDRPQIICTKPRRSHRMRRHHHHHQHHHQHRHHKSRKERRKHKKHPAEDWEKEEGNFC

Target ORF information:

RefSeq Version	XM_005208460.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurocan (NCAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208460.3

ORF Insert Sequence:

ATGGGGGCCT TGTCTGTCTG GGTCTCAGGC CTCCTGACTC TCCAGATGCT TCTCTTTGTG 
GCTGGGGAGC AGGGCCCACA GCACACCACC GCAGCTGCAG CCACTGCTGA GAAGGGGCTC 
CACATGCAGA AAGTGGGGTC TGGGTCAGTC CGGGCTGCCC TGGCAGAGTT GGTGGCCCTG 
CCCTGTCTCT TTAGCCTGCG GCCACGGCCA GGTGCAGCTC GAGAAGCCCC ACGGATCAAA 
TGGACCAAGG TGCGGACTGC ATCAGGCCAG CGGCAGGACT TGCCCATCCT AGTAGCCAAG 
GACAACGTGG TCCGAGTCGC CAAGGGCTGG CAGGGACGTG TGTCACTTCC CGCCTACCCC 
CGGCACCGGG TCAACGCCAC GTTGCTGCTG GGACCACTGA GGGCCAGCGA CTCCGGGCTC 
TACCGCTGCC AGGTGGTGAG GGGCATCGAG GATGAGCAGG ACCTGGTGCC CCTGGAGGTG 
ACGGGTGTTG TGTTCCACTA CCGAGCAGCC CTGGATCGCT ACTCTCTAAC TTTTGCTGAG 
GCTCAGGAGG CCTGTCTTCT CAACTCAGCC ACCATTGCAG CCCCGCGGCA CCTGCAGGCT 
GCCTTTGAGG ACGGCTTTGA CAACTGCGAT GCCGGGTGGC TTTCTGACCG CACTGTTCGG 
TATCCTATCA CCCAGTCCCG TCCTGGTTGC TATGGTGACC GTAGCAGCCT TCCGGGGGTG 
AGGAACTATG GGAGGCGAGA CCCACGGGAA CTCTACGATG TGTATTGCTT TGCCCGTGAG 
CTGGGGGGCG AGGTCTTCTA CGTAGGCCTG GCCCGCCGCC TGACACTGGC CGGCGCACGT 
GCCCAGTGCC GACGCCAGGG CGCCGTGCTG GCCTCGGTAG GACAGCTGCA CCTGGCCTGG 
CATGAGGGCC TGGACCAGTG CGACCCGGGC TGGCTGGCCG ACGGCAGCGT GCGCTACCCG 
ATCCAGACGC CGCGTCGGCG CTGCGGTGGC CCAGCCCCTG GCGTGCGCAC CGTATACCGC 
TTTGCCAACC GCACCGGCTT CCCGGCACCT GGAGAGCGCT TCGATGCCTA CTGCTTCAGA 
GCTCATCACC CCACATCCCA ACATGGAGAC CCAGAGACCC CCTCATCTGG AGATGAGGGG 
GAGATTCTGT CGGCAGAAGG GCCCCCCGCT GGAGAATTGG AGCCCAGTCT GCAGGAGGAG 
GAGGAGGTAG TCACCCCGGA CATCCAGGAG CCTCTGGTGT CTAGTGGGGA AGAAGAGTCT 
CTGATCTTGG CAGAGAAGCA GGAATCTCAA GCGACCCCCA GCCGTGCCCC TGGGGTCCCC 
ACATTGGCCT CAAGTCCTGC CCTGGAGGCT GAGGAGGTGT GGCTGAGCAC AGTGGCCCCC 
AGCCACAGCA GCGTGGAGGC AGGCACTGTG GTGGGCACGC ACACAGACAC AGCCCCAGCA 
AGCACCACGC CCAGGAAGAG GGAGCGCTTT AAAGGGCTGA ACGGGCGCCA CTTCCAGCAA 
CAGGAGCCCC AGCAAGGGCT GAAGGGGGCA CTCGAGGCCA GCATCCAGCC CCCCACACCA 
GAGGCTGCTG AGAATTATGT GGAGCCTCCC ATGACCACTG GAACCACAGA CGCCTTGGGG 
AGCGGCCAGA GCCAAAGCCC CTGGGCTGTG CTGACAAATG AGGTGGAAAT GCCCAGAGCT 
GGTTCCCTTG GTGGCAGGAG CCCCCAAGAG CCCTGGCTGT GGCTCCCAAC CGTGGTCCCA 
CCCATCATCC CAGACCCCAG CAGAGCCCTT GGCCTGGAAC TAGGAGAAGC CAAGCGCTTT 
AGTATGAGAC CAGTCACTTC CGACCTGCCC TGGTCCCCCT CAGAGACCAC TCCCAACCCT 
TCCAATGGCC CCAGCCCTAC CCCCTGGAAG GCTTCTCCTG TGGCCATTTC TCCAGACCTC 
CCTGTCATGG CCATGCTTCG CGCCCCCAAA CTGTGGCTCC TATCACAGGA GCCTCCTACA 
TCCCTCCCCA CTGAGGCCAG TGGAGTCAAG GGGCATAGTG AGGCCATGGC CACTGCCCTG 
CCCTCCCCTG CCTCAGAAAT CGAAGCCAGC CCACAGGATC CCATCCATCT GGAAGTGTAC 
TCCCCGCCTT CCTCCTCGGG CCTGGCAGGA CAAGATGGAG AGTCCACATC CCTGTCACTC 
AGCAGCCACA GAGGAGGAAG TCCTCCGGCC CCTTCACAGG CAGCCACAGA CACACAAGCT 
GGAGCCAGTC CCAACTCCCC TGGAGCAGAC TTTGGAAACA CTAGAGGGGC CAGCCCTACT 
GAGCTCAGCA AAGCTGAGCA CCCCGGATCC AGCCCACAGG CCTCTGTGGA CTGGAATGTG 
GTGGCAGATA TCACTATTTC TGAGCCTGCC ACTGAGCCCA TGGGAGCCAG AGGCGTCTCG 
GAGTCTGAGT CTGGGGTCTT CAACACAGCA GAGAGTCCTA CTTCCAGCTT GCAGGCCTCC 
ATGGACAAGA CGCAGGAAAT ATGGGCCTCG CTGCACAGGG AAGGGCTGGA CCCCCACTCC 
CCATCTGCAC CCTTGGGGGT CCCTGAAGTC CCCTTGATGC CCAAGTTCAC CCCAGGTTTG 
GGGCCTTGGG CTGCTACAGA TGGAGGAGTC ACAGTGGATC CTATGGCTTC CACAGTCACA 
CTGGACACCA GCGATGCTGG TGGGCATTGG GAACCTGGAT CCCACATGGT TGAGGGAGCA 
GAAAGCCCCA CCTTGAGCCC TCAAATGGCT GTGGACACAA GTGTGGTGAC ATCCGTCACG 
TCCTTGGACC TGGGGGACAA GGTCAGGGTC CTGGCCGTGT TCACAGTGGC CTCCTCAAGC 
TCCCAGCCCC ACCCAGAGCC AGAGAGCCAG CTGGTGACCC AGGGAACCCT GGGAGCCTTG 
GAGCCTCCAC AGGAGGGCAG CCACTCAGGG GACCCCATTC TTCCTCCTTG GACGCCGACA 
GCAGCCAACA AGGATGAGCC CATTTCAGTT TCCTCGGGGG AGCCTACAGT GCCTTGGGAC 
TCCCCCAGCA CCCTGCTGCC TGCCTCTCTG GGCCCAGAGG AATTTGAGCT GGAGGTCCTG 
ATGGGGAGCC CCAGTGTGGA GAGCTTCTGG GAGGAGGCAG CAAGTGGAGA GGAGCCTGCC 
CTGCCAGGGA CTCCTGCAAA TGGGAGTGCA GAGGAGGGCC CCTTGGATCC CTGTGAGAAC 
AACCCTTGCC TGCACGGAGG CACCTGTAGA GCCAATGGCA CCATGTACGG CTGCAGCTGT 
GACCAGGGGT TCGCTGGAGA GAACTGTGAG ATCGATATTG ACGATTGCAT CTCCAGCCCC 
TGTGAGAACG GTGGCACCTG CATCGACGAG GTCAACGCCT TCATCTGCCT TTGCCTCCCC 
AGCTATGGGG GAAGCCTCTG CGAGAAAGAT ACAGAGGGCT GTGACCACGG CTGGCACAAG 
TTTCAGGGCC ACTGCTACCG TTACTTTGCC CACCGGCGGG CATGGGAGGA CGCCGAGAGG 
GACTGCCGCC GCCGAGCCGG CCACCTAACC AGCATCCACT CATCAGAAGA ACACAACTTC 
ATTAACAGTT TTGGGCGTGA AAACACATGG ATCGGCTTGA ACGACAGGAT CGTGGAAAGG 
GATTTCCAGT GGACGGACAA CACAGGGCTG CAATTTGAGA ACTGGCGGGA AAATCAGCCG 
GACAACTTCT TCGCGGGCGG GGAGGACTGT GTGGTGATGG TTGCACATGA GAGCGGGCGC 
TGGAACGACG TTCCCTGCAA CTACAACCTC CCCTACGTCT GTAAGAAGGG CACAGTGCTG 
TGTGGTCCCC CTCCAGCGGT GGAGAATGCC TCGCCCATTG GTGCCCGCAA GGCCAAGTAT 
AATGTCCATG CCACAGTACG CTACCAGTGT GATGAAGGAT TTGCCCAGCA CCATGTGGCC 
ATCATCCGGT GCCGGAGCAA TGGCAAATGG GACCGGCCCC AGATCATCTG CACCAAACCC 
AGAAGGTCCC ATAGGATGCG GCGACACCAC CATCACCACC AGCATCACCA CCAGCATCGC 
CACCACAAAT CTCGCAAGGA GCGCAGAAAA CACAAGAAGC ACCCAGCAGA GGACTGGGAA 
AAGGAAGAAG GGAATTTCTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCAN ( XM_005208460.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208517.1 Bos taurus neurocan (NCAN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20802
Clone ID Related Accession (Same CDS sequence)	XM_005208460.3 , XM_005208460.4
Accession Version	XM_005208460.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurocan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208460.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCT TGTCTGTCTG GGTCTCAGGC CTCCTGACTC TCCAGATGCT TCTCTTTGTG 
GCTGGGGAGC AGGGCCCACA GCACACCACC GCAGCTGCAG CCACTGCTGA GAAGGGGCTC 
CACATGCAGA AAGTGGGGTC TGGGTCAGTC CGGGCTGCCC TGGCAGAGTT GGTGGCCCTG 
CCCTGTCTCT TTAGCCTGCG GCCACGGCCA GGTGCAGCTC GAGAAGCCCC ACGGATCAAA 
TGGACCAAGG TGCGGACTGC ATCAGGCCAG CGGCAGGACT TGCCCATCCT AGTAGCCAAG 
GACAACGTGG TCCGAGTCGC CAAGGGCTGG CAGGGACGTG TGTCACTTCC CGCCTACCCC 
CGGCACCGGG TCAACGCCAC GTTGCTGCTG GGACCACTGA GGGCCAGCGA CTCCGGGCTC 
TACCGCTGCC AGGTGGTGAG GGGCATCGAG GATGAGCAGG ACCTGGTGCC CCTGGAGGTG 
ACGGGTGTTG TGTTCCACTA CCGAGCAGCC CTGGATCGCT ACTCTCTAAC TTTTGCTGAG 
GCTCAGGAGG CCTGTCTTCT CAACTCAGCC ACCATTGCAG CCCCGCGGCA CCTGCAGGCT 
GCCTTTGAGG ACGGCTTTGA CAACTGCGAT GCCGGGTGGC TTTCTGACCG CACTGTTCGG 
TATCCTATCA CCCAGTCCCG TCCTGGTTGC TATGGTGACC GTAGCAGCCT TCCGGGGGTG 
AGGAACTATG GGAGGCGAGA CCCACGGGAA CTCTACGATG TGTATTGCTT TGCCCGTGAG 
CTGGGGGGCG AGGTCTTCTA CGTAGGCCTG GCCCGCCGCC TGACACTGGC CGGCGCACGT 
GCCCAGTGCC GACGCCAGGG CGCCGTGCTG GCCTCGGTAG GACAGCTGCA CCTGGCCTGG 
CATGAGGGCC TGGACCAGTG CGACCCGGGC TGGCTGGCCG ACGGCAGCGT GCGCTACCCG 
ATCCAGACGC CGCGTCGGCG CTGCGGTGGC CCAGCCCCTG GCGTGCGCAC CGTATACCGC 
TTTGCCAACC GCACCGGCTT CCCGGCACCT GGAGAGCGCT TCGATGCCTA CTGCTTCAGA 
GCTCATCACC CCACATCCCA ACATGGAGAC CCAGAGACCC CCTCATCTGG AGATGAGGGG 
GAGATTCTGT CGGCAGAAGG GCCCCCCGCT GGAGAATTGG AGCCCAGTCT GCAGGAGGAG 
GAGGAGGTAG TCACCCCGGA CATCCAGGAG CCTCTGGTGT CTAGTGGGGA AGAAGAGTCT 
CTGATCTTGG CAGAGAAGCA GGAATCTCAA GCGACCCCCA GCCGTGCCCC TGGGGTCCCC 
ACATTGGCCT CAAGTCCTGC CCTGGAGGCT GAGGAGGTGT GGCTGAGCAC AGTGGCCCCC 
AGCCACAGCA GCGTGGAGGC AGGCACTGTG GTGGGCACGC ACACAGACAC AGCCCCAGCA 
AGCACCACGC CCAGGAAGAG GGAGCGCTTT AAAGGGCTGA ACGGGCGCCA CTTCCAGCAA 
CAGGAGCCCC AGCAAGGGCT GAAGGGGGCA CTCGAGGCCA GCATCCAGCC CCCCACACCA 
GAGGCTGCTG AGAATTATGT GGAGCCTCCC ATGACCACTG GAACCACAGA CGCCTTGGGG 
AGCGGCCAGA GCCAAAGCCC CTGGGCTGTG CTGACAAATG AGGTGGAAAT GCCCAGAGCT 
GGTTCCCTTG GTGGCAGGAG CCCCCAAGAG CCCTGGCTGT GGCTCCCAAC CGTGGTCCCA 
CCCATCATCC CAGACCCCAG CAGAGCCCTT GGCCTGGAAC TAGGAGAAGC CAAGCGCTTT 
AGTATGAGAC CAGTCACTTC CGACCTGCCC TGGTCCCCCT CAGAGACCAC TCCCAACCCT 
TCCAATGGCC CCAGCCCTAC CCCCTGGAAG GCTTCTCCTG TGGCCATTTC TCCAGACCTC 
CCTGTCATGG CCATGCTTCG CGCCCCCAAA CTGTGGCTCC TATCACAGGA GCCTCCTACA 
TCCCTCCCCA CTGAGGCCAG TGGAGTCAAG GGGCATAGTG AGGCCATGGC CACTGCCCTG 
CCCTCCCCTG CCTCAGAAAT CGAAGCCAGC CCACAGGATC CCATCCATCT GGAAGTGTAC 
TCCCCGCCTT CCTCCTCGGG CCTGGCAGGA CAAGATGGAG AGTCCACATC CCTGTCACTC 
AGCAGCCACA GAGGAGGAAG TCCTCCGGCC CCTTCACAGG CAGCCACAGA CACACAAGCT 
GGAGCCAGTC CCAACTCCCC TGGAGCAGAC TTTGGAAACA CTAGAGGGGC CAGCCCTACT 
GAGCTCAGCA AAGCTGAGCA CCCCGGATCC AGCCCACAGG CCTCTGTGGA CTGGAATGTG 
GTGGCAGATA TCACTATTTC TGAGCCTGCC ACTGAGCCCA TGGGAGCCAG AGGCGTCTCG 
GAGTCTGAGT CTGGGGTCTT CAACACAGCA GAGAGTCCTA CTTCCAGCTT GCAGGCCTCC 
ATGGACAAGA CGCAGGAAAT ATGGGCCTCG CTGCACAGGG AAGGGCTGGA CCCCCACTCC 
CCATCTGCAC CCTTGGGGGT CCCTGAAGTC CCCTTGATGC CCAAGTTCAC CCCAGGTTTG 
GGGCCTTGGG CTGCTACAGA TGGAGGAGTC ACAGTGGATC CTATGGCTTC CACAGTCACA 
CTGGACACCA GCGATGCTGG TGGGCATTGG GAACCTGGAT CCCACATGGT TGAGGGAGCA 
GAAAGCCCCA CCTTGAGCCC TCAAATGGCT GTGGACACAA GTGTGGTGAC ATCCGTCACG 
TCCTTGGACC TGGGGGACAA GGTCAGGGTC CTGGCCGTGT TCACAGTGGC CTCCTCAAGC 
TCCCAGCCCC ACCCAGAGCC AGAGAGCCAG CTGGTGACCC AGGGAACCCT GGGAGCCTTG 
GAGCCTCCAC AGGAGGGCAG CCACTCAGGG GACCCCATTC TTCCTCCTTG GACGCCGACA 
GCAGCCAACA AGGATGAGCC CATTTCAGTT TCCTCGGGGG AGCCTACAGT GCCTTGGGAC 
TCCCCCAGCA CCCTGCTGCC TGCCTCTCTG GGCCCAGAGG AATTTGAGCT GGAGGTCCTG 
ATGGGGAGCC CCAGTGTGGA GAGCTTCTGG GAGGAGGCAG CAAGTGGAGA GGAGCCTGCC 
CTGCCAGGGA CTCCTGCAAA TGGGAGTGCA GAGGAGGGCC CCTTGGATCC CTGTGAGAAC 
AACCCTTGCC TGCACGGAGG CACCTGTAGA GCCAATGGCA CCATGTACGG CTGCAGCTGT 
GACCAGGGGT TCGCTGGAGA GAACTGTGAG ATCGATATTG ACGATTGCAT CTCCAGCCCC 
TGTGAGAACG GTGGCACCTG CATCGACGAG GTCAACGCCT TCATCTGCCT TTGCCTCCCC 
AGCTATGGGG GAAGCCTCTG CGAGAAAGAT ACAGAGGGCT GTGACCACGG CTGGCACAAG 
TTTCAGGGCC ACTGCTACCG TTACTTTGCC CACCGGCGGG CATGGGAGGA CGCCGAGAGG 
GACTGCCGCC GCCGAGCCGG CCACCTAACC AGCATCCACT CATCAGAAGA ACACAACTTC 
ATTAACAGTT TTGGGCGTGA AAACACATGG ATCGGCTTGA ACGACAGGAT CGTGGAAAGG 
GATTTCCAGT GGACGGACAA CACAGGGCTG CAATTTGAGA ACTGGCGGGA AAATCAGCCG 
GACAACTTCT TCGCGGGCGG GGAGGACTGT GTGGTGATGG TTGCACATGA GAGCGGGCGC 
TGGAACGACG TTCCCTGCAA CTACAACCTC CCCTACGTCT GTAAGAAGGG CACAGTGCTG 
TGTGGTCCCC CTCCAGCGGT GGAGAATGCC TCGCCCATTG GTGCCCGCAA GGCCAAGTAT 
AATGTCCATG CCACAGTACG CTACCAGTGT GATGAAGGAT TTGCCCAGCA CCATGTGGCC 
ATCATCCGGT GCCGGAGCAA TGGCAAATGG GACCGGCCCC AGATCATCTG CACCAAACCC 
AGAAGGTCCC ATAGGATGCG GCGACACCAC CATCACCACC AGCATCACCA CCAGCATCGC 
CACCACAAAT CTCGCAAGGA GCGCAGAAAA CACAAGAAGC ACCCAGCAGA GGACTGGGAA 
AAGGAAGAAG GGAATTTCTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208517.1
CDS	120..4223
Translation	MGALSVWVSGLLTLQMLLFVAGEQGPQHTTAAAATAEKGLHMQKVGSGSVRAALAELVALPCLFSLRPRPGAAREAPRIKWTKVRTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRVNATLLLGPLRASDSGLYRCQVVRGIEDEQDLVPLEVTGVVFHYRAALDRYSLTFAEAQEACLLNSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRNYGRRDPRELYDVYCFARELGGEVFYVGLARRLTLAGARAQCRRQGAVLASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGERFDAYCFRAHHPTSQHGDPETPSSGDEGEILSAEGPPAGELEPSLQEEEEVVTPDIQEPLVSSGEEESLILAEKQESQATPSRAPGVPTLASSPALEAEEVWLSTVAPSHSSVEAGTVVGTHTDTAPASTTPRKRERFKGLNGRHFQQQEPQQGLKGALEASIQPPTPEAAENYVEPPMTTGTTDALGSGQSQSPWAVLTNEVEMPRAGSLGGRSPQEPWLWLPTVVPPIIPDPSRALGLELGEAKRFSMRPVTSDLPWSPSETTPNPSNGPSPTPWKASPVAISPDLPVMAMLRAPKLWLLSQEPPTSLPTEASGVKGHSEAMATALPSPASEIEASPQDPIHLEVYSPPSSSGLAGQDGESTSLSLSSHRGGSPPAPSQAATDTQAGASPNSPGADFGNTRGASPTELSKAEHPGSSPQASVDWNVVADITISEPATEPMGARGVSESESGVFNTAESPTSSLQASMDKTQEIWASLHREGLDPHSPSAPLGVPEVPLMPKFTPGLGPWAATDGGVTVDPMASTVTLDTSDAGGHWEPGSHMVEGAESPTLSPQMAVDTSVVTSVTSLDLGDKVRVLAVFTVASSSSQPHPEPESQLVTQGTLGALEPPQEGSHSGDPILPPWTPTAANKDEPISVSSGEPTVPWDSPSTLLPASLGPEEFELEVLMGSPSVESFWEEAASGEEPALPGTPANGSAEEGPLDPCENNPCLHGGTCRANGTMYGCSCDQGFAGENCEIDIDDCISSPCENGGTCIDEVNAFICLCLPSYGGSLCEKDTEGCDHGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSIHSSEEHNFINSFGRENTWIGLNDRIVERDFQWTDNTGLQFENWRENQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASPIGARKAKYNVHATVRYQCDEGFAQHHVAIIRCRSNGKWDRPQIICTKPRRSHRMRRHHHHHQHHHQHRHHKSRKERRKHKKHPAEDWEKEEGNFC

Target ORF information:

RefSeq Version	XM_005208460.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neurocan (NCAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208460.4

ORF Insert Sequence:

ATGGGGGCCT TGTCTGTCTG GGTCTCAGGC CTCCTGACTC TCCAGATGCT TCTCTTTGTG 
GCTGGGGAGC AGGGCCCACA GCACACCACC GCAGCTGCAG CCACTGCTGA GAAGGGGCTC 
CACATGCAGA AAGTGGGGTC TGGGTCAGTC CGGGCTGCCC TGGCAGAGTT GGTGGCCCTG 
CCCTGTCTCT TTAGCCTGCG GCCACGGCCA GGTGCAGCTC GAGAAGCCCC ACGGATCAAA 
TGGACCAAGG TGCGGACTGC ATCAGGCCAG CGGCAGGACT TGCCCATCCT AGTAGCCAAG 
GACAACGTGG TCCGAGTCGC CAAGGGCTGG CAGGGACGTG TGTCACTTCC CGCCTACCCC 
CGGCACCGGG TCAACGCCAC GTTGCTGCTG GGACCACTGA GGGCCAGCGA CTCCGGGCTC 
TACCGCTGCC AGGTGGTGAG GGGCATCGAG GATGAGCAGG ACCTGGTGCC CCTGGAGGTG 
ACGGGTGTTG TGTTCCACTA CCGAGCAGCC CTGGATCGCT ACTCTCTAAC TTTTGCTGAG 
GCTCAGGAGG CCTGTCTTCT CAACTCAGCC ACCATTGCAG CCCCGCGGCA CCTGCAGGCT 
GCCTTTGAGG ACGGCTTTGA CAACTGCGAT GCCGGGTGGC TTTCTGACCG CACTGTTCGG 
TATCCTATCA CCCAGTCCCG TCCTGGTTGC TATGGTGACC GTAGCAGCCT TCCGGGGGTG 
AGGAACTATG GGAGGCGAGA CCCACGGGAA CTCTACGATG TGTATTGCTT TGCCCGTGAG 
CTGGGGGGCG AGGTCTTCTA CGTAGGCCTG GCCCGCCGCC TGACACTGGC CGGCGCACGT 
GCCCAGTGCC GACGCCAGGG CGCCGTGCTG GCCTCGGTAG GACAGCTGCA CCTGGCCTGG 
CATGAGGGCC TGGACCAGTG CGACCCGGGC TGGCTGGCCG ACGGCAGCGT GCGCTACCCG 
ATCCAGACGC CGCGTCGGCG CTGCGGTGGC CCAGCCCCTG GCGTGCGCAC CGTATACCGC 
TTTGCCAACC GCACCGGCTT CCCGGCACCT GGAGAGCGCT TCGATGCCTA CTGCTTCAGA 
GCTCATCACC CCACATCCCA ACATGGAGAC CCAGAGACCC CCTCATCTGG AGATGAGGGG 
GAGATTCTGT CGGCAGAAGG GCCCCCCGCT GGAGAATTGG AGCCCAGTCT GCAGGAGGAG 
GAGGAGGTAG TCACCCCGGA CATCCAGGAG CCTCTGGTGT CTAGTGGGGA AGAAGAGTCT 
CTGATCTTGG CAGAGAAGCA GGAATCTCAA GCGACCCCCA GCCGTGCCCC TGGGGTCCCC 
ACATTGGCCT CAAGTCCTGC CCTGGAGGCT GAGGAGGTGT GGCTGAGCAC AGTGGCCCCC 
AGCCACAGCA GCGTGGAGGC AGGCACTGTG GTGGGCACGC ACACAGACAC AGCCCCAGCA 
AGCACCACGC CCAGGAAGAG GGAGCGCTTT AAAGGGCTGA ACGGGCGCCA CTTCCAGCAA 
CAGGAGCCCC AGCAAGGGCT GAAGGGGGCA CTCGAGGCCA GCATCCAGCC CCCCACACCA 
GAGGCTGCTG AGAATTATGT GGAGCCTCCC ATGACCACTG GAACCACAGA CGCCTTGGGG 
AGCGGCCAGA GCCAAAGCCC CTGGGCTGTG CTGACAAATG AGGTGGAAAT GCCCAGAGCT 
GGTTCCCTTG GTGGCAGGAG CCCCCAAGAG CCCTGGCTGT GGCTCCCAAC CGTGGTCCCA 
CCCATCATCC CAGACCCCAG CAGAGCCCTT GGCCTGGAAC TAGGAGAAGC CAAGCGCTTT 
AGTATGAGAC CAGTCACTTC CGACCTGCCC TGGTCCCCCT CAGAGACCAC TCCCAACCCT 
TCCAATGGCC CCAGCCCTAC CCCCTGGAAG GCTTCTCCTG TGGCCATTTC TCCAGACCTC 
CCTGTCATGG CCATGCTTCG CGCCCCCAAA CTGTGGCTCC TATCACAGGA GCCTCCTACA 
TCCCTCCCCA CTGAGGCCAG TGGAGTCAAG GGGCATAGTG AGGCCATGGC CACTGCCCTG 
CCCTCCCCTG CCTCAGAAAT CGAAGCCAGC CCACAGGATC CCATCCATCT GGAAGTGTAC 
TCCCCGCCTT CCTCCTCGGG CCTGGCAGGA CAAGATGGAG AGTCCACATC CCTGTCACTC 
AGCAGCCACA GAGGAGGAAG TCCTCCGGCC CCTTCACAGG CAGCCACAGA CACACAAGCT 
GGAGCCAGTC CCAACTCCCC TGGAGCAGAC TTTGGAAACA CTAGAGGGGC CAGCCCTACT 
GAGCTCAGCA AAGCTGAGCA CCCCGGATCC AGCCCACAGG CCTCTGTGGA CTGGAATGTG 
GTGGCAGATA TCACTATTTC TGAGCCTGCC ACTGAGCCCA TGGGAGCCAG AGGCGTCTCG 
GAGTCTGAGT CTGGGGTCTT CAACACAGCA GAGAGTCCTA CTTCCAGCTT GCAGGCCTCC 
ATGGACAAGA CGCAGGAAAT ATGGGCCTCG CTGCACAGGG AAGGGCTGGA CCCCCACTCC 
CCATCTGCAC CCTTGGGGGT CCCTGAAGTC CCCTTGATGC CCAAGTTCAC CCCAGGTTTG 
GGGCCTTGGG CTGCTACAGA TGGAGGAGTC ACAGTGGATC CTATGGCTTC CACAGTCACA 
CTGGACACCA GCGATGCTGG TGGGCATTGG GAACCTGGAT CCCACATGGT TGAGGGAGCA 
GAAAGCCCCA CCTTGAGCCC TCAAATGGCT GTGGACACAA GTGTGGTGAC ATCCGTCACG 
TCCTTGGACC TGGGGGACAA GGTCAGGGTC CTGGCCGTGT TCACAGTGGC CTCCTCAAGC 
TCCCAGCCCC ACCCAGAGCC AGAGAGCCAG CTGGTGACCC AGGGAACCCT GGGAGCCTTG 
GAGCCTCCAC AGGAGGGCAG CCACTCAGGG GACCCCATTC TTCCTCCTTG GACGCCGACA 
GCAGCCAACA AGGATGAGCC CATTTCAGTT TCCTCGGGGG AGCCTACAGT GCCTTGGGAC 
TCCCCCAGCA CCCTGCTGCC TGCCTCTCTG GGCCCAGAGG AATTTGAGCT GGAGGTCCTG 
ATGGGGAGCC CCAGTGTGGA GAGCTTCTGG GAGGAGGCAG CAAGTGGAGA GGAGCCTGCC 
CTGCCAGGGA CTCCTGCAAA TGGGAGTGCA GAGGAGGGCC CCTTGGATCC CTGTGAGAAC 
AACCCTTGCC TGCACGGAGG CACCTGTAGA GCCAATGGCA CCATGTACGG CTGCAGCTGT 
GACCAGGGGT TCGCTGGAGA GAACTGTGAG ATCGATATTG ACGATTGCAT CTCCAGCCCC 
TGTGAGAACG GTGGCACCTG CATCGACGAG GTCAACGCCT TCATCTGCCT TTGCCTCCCC 
AGCTATGGGG GAAGCCTCTG CGAGAAAGAT ACAGAGGGCT GTGACCACGG CTGGCACAAG 
TTTCAGGGCC ACTGCTACCG TTACTTTGCC CACCGGCGGG CATGGGAGGA CGCCGAGAGG 
GACTGCCGCC GCCGAGCCGG CCACCTAACC AGCATCCACT CATCAGAAGA ACACAACTTC 
ATTAACAGTT TTGGGCGTGA AAACACATGG ATCGGCTTGA ACGACAGGAT CGTGGAAAGG 
GATTTCCAGT GGACGGACAA CACAGGGCTG CAATTTGAGA ACTGGCGGGA AAATCAGCCG 
GACAACTTCT TCGCGGGCGG GGAGGACTGT GTGGTGATGG TTGCACATGA GAGCGGGCGC 
TGGAACGACG TTCCCTGCAA CTACAACCTC CCCTACGTCT GTAAGAAGGG CACAGTGCTG 
TGTGGTCCCC CTCCAGCGGT GGAGAATGCC TCGCCCATTG GTGCCCGCAA GGCCAAGTAT 
AATGTCCATG CCACAGTACG CTACCAGTGT GATGAAGGAT TTGCCCAGCA CCATGTGGCC 
ATCATCCGGT GCCGGAGCAA TGGCAAATGG GACCGGCCCC AGATCATCTG CACCAAACCC 
AGAAGGTCCC ATAGGATGCG GCGACACCAC CATCACCACC AGCATCACCA CCAGCATCGC 
CACCACAAAT CTCGCAAGGA GCGCAGAAAA CACAAGAAGC ACCCAGCAGA GGACTGGGAA 
AAGGAAGAAG GGAATTTCTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NCAN cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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