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PRUNE1 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol PRUNE1
Entrez Gene ID 524973
Full Name prune exopolyphosphatase 1
Synonyms PRUNE
General protein information
Preferred Names

prune exopolyphosphatase 1

Names

exopolyphosphatase PRUNE1
protein prune homolog
prune homolog
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_010803029.3 XP_010801331.1 exopolyphosphatase PRUNE1 isoform X1
XM_015462708.2 XP_015318194.1 exopolyphosphatase PRUNE1 isoform X3
XM_015462709.2 XP_015318195.1 exopolyphosphatase PRUNE1 isoform X4
XM_010803028.2 XP_010801330.1 protein prune homolog isoform X1
XM_010803029.2 XP_010801331.1 protein prune homolog isoform X2
XM_015462708.1 XP_015318194.1 protein prune homolog isoform X3
XM_024989371.1 XP_024845139.1 exopolyphosphatase PRUNE1 isoform X1
XM_015462709.1 XP_015318195.1 protein prune homolog isoform X4
XM_024989372.1 XP_024845140.1 exopolyphosphatase PRUNE1 isoform X2
NM_001035352.1 NP_001030429.1 exopolyphosphatase PRUNE1

Pathways from BioSystems


Homologies


Homo sapiens (human) PRUNE NP_067045.1
Canis lupus familiaris (dog) PRUNE XP_540307.3
Mus musculus (house mouse) Prune NP_775482.1
Gallus gallus (chicken) PRUNE XP_004948377.1
Kluyveromyces lactis KLLA0B03806g XP_451703.1
Eremothecium gossypii AGOS_ABL083W NP_982864.1
Magnaporthe oryzae (rice blast fungus) MGG_08801 XP_003719170.1
Neurospora crassa NCU06310 XP_962572.1
LOC708898 XP_002808286.1
Rattus norvegicus (Norway rat) Prune NP_001007698.1
Danio rerio (zebrafish) LOC563343 NP_001153766.1
Saccharomyces cerevisiae (baker's yeast) PPX1 NP_012071.1
Bos taurus (cattle) PRUNE NP_001030429.1
Gallus gallus (chicken) LOC100857290 XP_004948402.1
Drosophila melanogaster (fruit fly) pn NP_476684.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004314 XP_313586.5
Schizosaccharomyces pombe (fission yeast) SPAC2F3.11 NP_594390.1
Xenopus tropicalis (tropical clawed frog) prune XP_002937310.1

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRUNE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRUNE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa17428 NM_001035352.1
Latest version!		 Bos taurus prune exopolyphosphatase 1 (PRUNE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa50935 XM_010803028.2
Latest version!		 Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa50936 XM_010803029.3
Latest version!		 Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa50936 XM_024989371.1
Latest version!		 Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa17429 XM_024989372.1
Latest version!		 Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa90396 XM_015462708.2
Latest version!		 Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa90397 XM_015462709.2
Latest version!		 Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
View Old Accession Versions >>
OBa50936 XM_010803029.2 Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa90396 XM_015462708.1 Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa90397 XM_015462709.1 Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa17428
Clone ID Related Accession (Same CDS sequence) NM_001035352.1
Accession Version NM_001035352.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Bos taurus(cattle)
Product exopolyphosphatase PRUNE1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT020783.1. On Nov 1, 2005 this sequence version replaced XM_603314.2. ##Evidence-Data-START## Transcript exon combination :: BT020783.1, BC142289.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGAACT ACCTGCAGGG TTGTCGAGCT GCTCTGCAGG AGTCCCGACC CATACATGTT 
GTGCTGGGAA ATGAAGCCTG TGACTTGGAC TCCATGGTGT CAGCCCTTGC CCTGGCTTTT 
TACCTGGCAA AGACAACTGA GGCGGAGGAA GTCTTTGTGC CAGTGTTAAA TATAAAACGT 
TCAGAGCTAC CTCTACGAGG TGACAATGTC TTCTTCCTTC AGAAGATTCA CATTCCAGAG 
TCCGTCTTGA TTTTCCGGGA TGAGATTGAC CTCCATGCAC TGCACCAGGC TGGCCAGCTC 
ACCCTCATCC TTGTTGACCA TCATGTCTTA CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA 
GTGGCAGAGG TGCTAGACCA TCGGCCCATC GATCAGAGAC ACTGCCCTCC CTGCCATGTT 
TCAGTTGAAC TGGTGGGGTC CTGCGCTACC CTGGTGGCCG AGAGAATCCT GCAGGGGGCA 
CCAGAGATCT TGGACAGGCA AACTGCAGCC CTTCTTCATG GAACAATCCT CCTGGACTGT 
GTGAACATGG ACCTTAAGAT TGGAAAGGCA ACCCTCAAGG ACAGCCACTA TGTAGAGAAG 
CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC AGAAATGACA TCTTTGATTC TCTGCAAAAG 
GCCAAGTTTG ATGTATCAGG ACTGACCACA GAGCAGATGC TGAGAAAGGA CCAGAAGACC 
ATCTCCAGAC AAGGCACTAA GGTGGCCATT AGTGCAATAT ATATGGATAT GGAGGCTTTT 
CTGCAGAGGT CTGGCCTCCT TGCAGATCTC CGTGCCTTCT GCCAGGCTCA TAGCTACGAT 
GCCCTGGTTG CCATGACTAT CTTTTTCAAC ACTTACAATG AGCCAGTGCG GCAGTTGGCT 
GTTTTCTGTC CCCACGCAGC ACTTCGAATG ACGATCTGTG GAATCCTGGA ACATTCCCAC 
TCTCCACCCC TGAAGCTGAC CCCTGTCCCA AGCAGCCACC CTGACCTCCA AGCCTATCTT 
CAAGGCAACA CCCAGATCTC TCGAAAGAAA GTTCTGCCTC TGCTCCAGGA AGCCCTGTCA 
GCGTATTTTG ACTCCACGAA CATCCCTTTA GGACAGCCTG AGACAGAGGG TGTGTCAAGG 
GAACAGGTGG ACAAAGAATT GGACAGGGCA GGCAACTCCC TGCTTTCTGG ACTGAGTCAG 
GATGAGGAGG AGCCTCCACT GCCCCCCACG CCCATGAACA GCCTGGTGGA CGAGTGCCCC 
CTGGATCAGG GGCTGCCGAA GTTCTCAGCC GAAGTCATCT TCGAGAAATG TAGTCAGATC 
TCGCTGTCAG AACCTACCAC TGCCTCCCTG TCTAAGAAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001030429.1
CDS4..1365
Translation

Target ORF information:

RefSeq Version NM_001035352.1
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase 1 (PRUNE1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001035352.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGAACT ACCTGCAGGG TTGTCGAGCT GCTCTGCAGG AGTCCCGACC CATACATGTT 
GTGCTGGGAA ATGAAGCCTG TGACTTGGAC TCCATGGTGT CAGCCCTTGC CCTGGCTTTT 
TACCTGGCAA AGACAACTGA GGCGGAGGAA GTCTTTGTGC CAGTGTTAAA TATAAAACGT 
TCAGAGCTAC CTCTACGAGG TGACAATGTC TTCTTCCTTC AGAAGATTCA CATTCCAGAG 
TCCGTCTTGA TTTTCCGGGA TGAGATTGAC CTCCATGCAC TGCACCAGGC TGGCCAGCTC 
ACCCTCATCC TTGTTGACCA TCATGTCTTA CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA 
GTGGCAGAGG TGCTAGACCA TCGGCCCATC GATCAGAGAC ACTGCCCTCC CTGCCATGTT 
TCAGTTGAAC TGGTGGGGTC CTGCGCTACC CTGGTGGCCG AGAGAATCCT GCAGGGGGCA 
CCAGAGATCT TGGACAGGCA AACTGCAGCC CTTCTTCATG GAACAATCCT CCTGGACTGT 
GTGAACATGG ACCTTAAGAT TGGAAAGGCA ACCCTCAAGG ACAGCCACTA TGTAGAGAAG 
CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC AGAAATGACA TCTTTGATTC TCTGCAAAAG 
GCCAAGTTTG ATGTATCAGG ACTGACCACA GAGCAGATGC TGAGAAAGGA CCAGAAGACC 
ATCTCCAGAC AAGGCACTAA GGTGGCCATT AGTGCAATAT ATATGGATAT GGAGGCTTTT 
CTGCAGAGGT CTGGCCTCCT TGCAGATCTC CGTGCCTTCT GCCAGGCTCA TAGCTACGAT 
GCCCTGGTTG CCATGACTAT CTTTTTCAAC ACTTACAATG AGCCAGTGCG GCAGTTGGCT 
GTTTTCTGTC CCCACGCAGC ACTTCGAATG ACGATCTGTG GAATCCTGGA ACATTCCCAC 
TCTCCACCCC TGAAGCTGAC CCCTGTCCCA AGCAGCCACC CTGACCTCCA AGCCTATCTT 
CAAGGCAACA CCCAGATCTC TCGAAAGAAA GTTCTGCCTC TGCTCCAGGA AGCCCTGTCA 
GCGTATTTTG ACTCCACGAA CATCCCTTTA GGACAGCCTG AGACAGAGGG TGTGTCAAGG 
GAACAGGTGG ACAAAGAATT GGACAGGGCA GGCAACTCCC TGCTTTCTGG ACTGAGTCAG 
GATGAGGAGG AGCCTCCACT GCCCCCCACG CCCATGAACA GCCTGGTGGA CGAGTGCCCC 
CTGGATCAGG GGCTGCCGAA GTTCTCAGCC GAAGTCATCT TCGAGAAATG TAGTCAGATC 
TCGCTGTCAG AACCTACCAC TGCCTCCCTG TCTAAGAAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa50935
Clone ID Related Accession (Same CDS sequence) XM_010803028.2
Accession Version XM_010803028.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1332bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product protein prune homolog isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCAGAAGG AGTCCCGACC CATACATGTT GTGCTGGGAA ATGAAGCCTG TGACTTGGAC 
TCCATGGTGT CAGCCCTTGC CCTGGCTTTT TACCTGGCAA AGACAACTGA GGCGGAGGAA 
GTCTTTGTGC CAGTGTTAAA TATAAAACGT TCAGAGCTAC CTCTACGAGG TGACAATGTC 
TTCTTCCTTC AGAAGATTCA CATTCCAGAG TCCGTCTTGA TTTTCCGGGA TGAGATTGAC 
CTCCATGCAC TGCACCAGGC TGGCCAGCTC ACCCTCATCC TTGTTGACCA TCATGTCTTA 
CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA GTGGCAGAGG TGCTAGACCA TCGGCCCATC 
GATCAGAGAC ACTGCCCTCC CTGCCATGTT TCAGTTGAAC TGGTGGGGTC CTGCGCTACC 
CTGGTGGCCG AGAGAATCCT GCAGGGGGCA CCAGAGATCT TGGACAGGCA AACTGCAGCC 
CTTCTTCATG GAACAATCCT CCTGGACTGT GTGAACATGG ACCTTAAGAT TGGAAAGGCA 
ACCCTCAAGG ACAGCCACTA TGTAGAGAAG CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC 
AGAAATGACA TCTTTGATTC TCTGCAAAAG GCCAAGTTTG ATGTATCAGG ACTGACCACA 
GAGCAGATGC TGAGAAAGGA CCAGAAGACC ATCTCCAGAC AAGGCACTAA GGTGGCCATT 
AGTGCAATAT ATATGGATAT GGAGGCTTTT CTGCAGAGGT CTGGCCTCCT TGCAGATCTC 
CGTGCCTTCT GCCAGGCTCA TAGCTACGAT GCCCTGGTTG CCATGACTAT CTTTTTCAAC 
ACTTACAATG AGCCAGTGCG GCAGTTGGCT GTTTTCTGTC CCCACGCAGC ACTTCGAATG 
ACGATCTGTG GAATCCTGGA ACATTCCCAC TCTCCACCCC TGAAGCTGAC CCCTGTCCCA 
AGCAGCCACC CTGACCTCCA AGCCTATCTT CAAGGCAACA CCCAGATCTC TCGAAAGAAA 
GTTCTGCCTC TGCTCCAGGA AGCCCTGTCA GCGTATTTTG ACTCCACGAA CATCCCTTTA 
GGACAGCCTG AGACAGAGGG TGTGTCAAGG GAACAGGTGG ACAAAGAATT GGACAGGGCA 
GGCAACTCCC TGCTTTCTGG ACTGAGTCAG GATGAGGAGG AGCCTCCACT GCCCCCCACG 
CCCATGAACA GCCTGGTGGA CGAGTGCCCC CTGGATCAGG GGCTGCCGAA GTTCTCAGCC 
GAAGTCATCT TCGAGAAATG TAGTCAGATC TCGCTGTCAG AACCTACCAC TGCCTCCCTG 
TCTAAGAAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010801330.1
CDS132..1463
Translation

Target ORF information:

RefSeq Version XM_010803028.2
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010803028.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCAGAAGG AGTCCCGACC CATACATGTT GTGCTGGGAA ATGAAGCCTG TGACTTGGAC 
TCCATGGTGT CAGCCCTTGC CCTGGCTTTT TACCTGGCAA AGACAACTGA GGCGGAGGAA 
GTCTTTGTGC CAGTGTTAAA TATAAAACGT TCAGAGCTAC CTCTACGAGG TGACAATGTC 
TTCTTCCTTC AGAAGATTCA CATTCCAGAG TCCGTCTTGA TTTTCCGGGA TGAGATTGAC 
CTCCATGCAC TGCACCAGGC TGGCCAGCTC ACCCTCATCC TTGTTGACCA TCATGTCTTA 
CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA GTGGCAGAGG TGCTAGACCA TCGGCCCATC 
GATCAGAGAC ACTGCCCTCC CTGCCATGTT TCAGTTGAAC TGGTGGGGTC CTGCGCTACC 
CTGGTGGCCG AGAGAATCCT GCAGGGGGCA CCAGAGATCT TGGACAGGCA AACTGCAGCC 
CTTCTTCATG GAACAATCCT CCTGGACTGT GTGAACATGG ACCTTAAGAT TGGAAAGGCA 
ACCCTCAAGG ACAGCCACTA TGTAGAGAAG CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC 
AGAAATGACA TCTTTGATTC TCTGCAAAAG GCCAAGTTTG ATGTATCAGG ACTGACCACA 
GAGCAGATGC TGAGAAAGGA CCAGAAGACC ATCTCCAGAC AAGGCACTAA GGTGGCCATT 
AGTGCAATAT ATATGGATAT GGAGGCTTTT CTGCAGAGGT CTGGCCTCCT TGCAGATCTC 
CGTGCCTTCT GCCAGGCTCA TAGCTACGAT GCCCTGGTTG CCATGACTAT CTTTTTCAAC 
ACTTACAATG AGCCAGTGCG GCAGTTGGCT GTTTTCTGTC CCCACGCAGC ACTTCGAATG 
ACGATCTGTG GAATCCTGGA ACATTCCCAC TCTCCACCCC TGAAGCTGAC CCCTGTCCCA 
AGCAGCCACC CTGACCTCCA AGCCTATCTT CAAGGCAACA CCCAGATCTC TCGAAAGAAA 
GTTCTGCCTC TGCTCCAGGA AGCCCTGTCA GCGTATTTTG ACTCCACGAA CATCCCTTTA 
GGACAGCCTG AGACAGAGGG TGTGTCAAGG GAACAGGTGG ACAAAGAATT GGACAGGGCA 
GGCAACTCCC TGCTTTCTGG ACTGAGTCAG GATGAGGAGG AGCCTCCACT GCCCCCCACG 
CCCATGAACA GCCTGGTGGA CGAGTGCCCC CTGGATCAGG GGCTGCCGAA GTTCTCAGCC 
GAAGTCATCT TCGAGAAATG TAGTCAGATC TCGCTGTCAG AACCTACCAC TGCCTCCCTG 
TCTAAGAAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa50936
Clone ID Related Accession (Same CDS sequence) XM_010803029.3 , XM_010803029.2 , XM_024989371.1
Accession Version XM_010803029.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product exopolyphosphatase PRUNE1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTGTCAG CCCTTGCCCT GGCTTTTTAC CTGGCAAAGA CAACTGAGGC GGAGGAAGTC 
TTTGTGCCAG TGTTAAATAT AAAACGTTCA GAGCTACCTC TACGAGGTGA CAATGTCTTC 
TTCCTTCAGA AGATTCACAT TCCAGAGTCC GTCTTGATTT TCCGGGATGA GATTGACCTC 
CATGCACTGC ACCAGGCTGG CCAGCTCACC CTCATCCTTG TTGACCATCA TGTCTTACCC 
AAAAGTGACG CAGCCCTAGA GGAGGCAGTG GCAGAGGTGC TAGACCATCG GCCCATCGAT 
CAGAGACACT GCCCTCCCTG CCATGTTTCA GTTGAACTGG TGGGGTCCTG CGCTACCCTG 
GTGGCCGAGA GAATCCTGCA GGGGGCACCA GAGATCTTGG ACAGGCAAAC TGCAGCCCTT 
CTTCATGGAA CAATCCTCCT GGACTGTGTG AACATGGACC TTAAGATTGG AAAGGCAACC 
CTCAAGGACA GCCACTATGT AGAGAAGCTG GAGGCCCTCT TCCCAGATCT GCCCAGCAGA 
AATGACATCT TTGATTCTCT GCAAAAGGCC AAGTTTGATG TATCAGGACT GACCACAGAG 
CAGATGCTGA GAAAGGACCA GAAGACCATC TCCAGACAAG GCACTAAGGT GGCCATTAGT 
GCAATATATA TGGATATGGA GGCTTTTCTG CAGAGGTCTG GCCTCCTTGC AGATCTCCGT 
GCCTTCTGCC AGGCTCATAG CTACGATGCC CTGGTTGCCA TGACTATCTT TTTCAACACT 
TACAATGAGC CAGTGCGGCA GTTGGCTGTT TTCTGTCCCC ACGCAGCACT TCGAATGACG 
ATCTGTGGAA TCCTGGAACA TTCCCACTCT CCACCCCTGA AGCTGACCCC TGTCCCAAGC 
AGCCACCCTG ACCTCCAAGC CTATCTTCAA GGCAACACCC AGATCTCTCG AAAGAAAGTT 
CTGCCTCTGC TCCAGGAAGC CCTGTCAGCG TATTTTGACT CCACGAACAT CCCTTTAGGA 
CAGCCTGAGA CAGAGGGTGT GTCAAGGGAA CAGGTGGACA AAGAATTGGA CAGGGCAGGC 
AACTCCCTGC TTTCTGGACT GAGTCAGGAT GAGGAGGAGC CTCCACTGCC CCCCACGCCC 
ATGAACAGCC TGGTGGACGA GTGCCCCCTG GATCAGGGGC TGCCGAAGTT CTCAGCCGAA 
GTCATCTTCG AGAAATGTAG TCAGATCTCG CTGTCAGAAC CTACCACTGC CTCCCTGTCT 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010801331.1
CDS191..1459
Translation

Target ORF information:

RefSeq Version XM_010803029.3
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010803029.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTGTCAG CCCTTGCCCT GGCTTTTTAC CTGGCAAAGA CAACTGAGGC GGAGGAAGTC 
TTTGTGCCAG TGTTAAATAT AAAACGTTCA GAGCTACCTC TACGAGGTGA CAATGTCTTC 
TTCCTTCAGA AGATTCACAT TCCAGAGTCC GTCTTGATTT TCCGGGATGA GATTGACCTC 
CATGCACTGC ACCAGGCTGG CCAGCTCACC CTCATCCTTG TTGACCATCA TGTCTTACCC 
AAAAGTGACG CAGCCCTAGA GGAGGCAGTG GCAGAGGTGC TAGACCATCG GCCCATCGAT 
CAGAGACACT GCCCTCCCTG CCATGTTTCA GTTGAACTGG TGGGGTCCTG CGCTACCCTG 
GTGGCCGAGA GAATCCTGCA GGGGGCACCA GAGATCTTGG ACAGGCAAAC TGCAGCCCTT 
CTTCATGGAA CAATCCTCCT GGACTGTGTG AACATGGACC TTAAGATTGG AAAGGCAACC 
CTCAAGGACA GCCACTATGT AGAGAAGCTG GAGGCCCTCT TCCCAGATCT GCCCAGCAGA 
AATGACATCT TTGATTCTCT GCAAAAGGCC AAGTTTGATG TATCAGGACT GACCACAGAG 
CAGATGCTGA GAAAGGACCA GAAGACCATC TCCAGACAAG GCACTAAGGT GGCCATTAGT 
GCAATATATA TGGATATGGA GGCTTTTCTG CAGAGGTCTG GCCTCCTTGC AGATCTCCGT 
GCCTTCTGCC AGGCTCATAG CTACGATGCC CTGGTTGCCA TGACTATCTT TTTCAACACT 
TACAATGAGC CAGTGCGGCA GTTGGCTGTT TTCTGTCCCC ACGCAGCACT TCGAATGACG 
ATCTGTGGAA TCCTGGAACA TTCCCACTCT CCACCCCTGA AGCTGACCCC TGTCCCAAGC 
AGCCACCCTG ACCTCCAAGC CTATCTTCAA GGCAACACCC AGATCTCTCG AAAGAAAGTT 
CTGCCTCTGC TCCAGGAAGC CCTGTCAGCG TATTTTGACT CCACGAACAT CCCTTTAGGA 
CAGCCTGAGA CAGAGGGTGT GTCAAGGGAA CAGGTGGACA AAGAATTGGA CAGGGCAGGC 
AACTCCCTGC TTTCTGGACT GAGTCAGGAT GAGGAGGAGC CTCCACTGCC CCCCACGCCC 
ATGAACAGCC TGGTGGACGA GTGCCCCCTG GATCAGGGGC TGCCGAAGTT CTCAGCCGAA 
GTCATCTTCG AGAAATGTAG TCAGATCTCG CTGTCAGAAC CTACCACTGC CTCCCTGTCT 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa50936
Clone ID Related Accession (Same CDS sequence) XM_010803029.3 , XM_010803029.2 , XM_024989371.1
Accession Version XM_010803029.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product protein prune homolog isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTGTCAG CCCTTGCCCT GGCTTTTTAC CTGGCAAAGA CAACTGAGGC GGAGGAAGTC 
TTTGTGCCAG TGTTAAATAT AAAACGTTCA GAGCTACCTC TACGAGGTGA CAATGTCTTC 
TTCCTTCAGA AGATTCACAT TCCAGAGTCC GTCTTGATTT TCCGGGATGA GATTGACCTC 
CATGCACTGC ACCAGGCTGG CCAGCTCACC CTCATCCTTG TTGACCATCA TGTCTTACCC 
AAAAGTGACG CAGCCCTAGA GGAGGCAGTG GCAGAGGTGC TAGACCATCG GCCCATCGAT 
CAGAGACACT GCCCTCCCTG CCATGTTTCA GTTGAACTGG TGGGGTCCTG CGCTACCCTG 
GTGGCCGAGA GAATCCTGCA GGGGGCACCA GAGATCTTGG ACAGGCAAAC TGCAGCCCTT 
CTTCATGGAA CAATCCTCCT GGACTGTGTG AACATGGACC TTAAGATTGG AAAGGCAACC 
CTCAAGGACA GCCACTATGT AGAGAAGCTG GAGGCCCTCT TCCCAGATCT GCCCAGCAGA 
AATGACATCT TTGATTCTCT GCAAAAGGCC AAGTTTGATG TATCAGGACT GACCACAGAG 
CAGATGCTGA GAAAGGACCA GAAGACCATC TCCAGACAAG GCACTAAGGT GGCCATTAGT 
GCAATATATA TGGATATGGA GGCTTTTCTG CAGAGGTCTG GCCTCCTTGC AGATCTCCGT 
GCCTTCTGCC AGGCTCATAG CTACGATGCC CTGGTTGCCA TGACTATCTT TTTCAACACT 
TACAATGAGC CAGTGCGGCA GTTGGCTGTT TTCTGTCCCC ACGCAGCACT TCGAATGACG 
ATCTGTGGAA TCCTGGAACA TTCCCACTCT CCACCCCTGA AGCTGACCCC TGTCCCAAGC 
AGCCACCCTG ACCTCCAAGC CTATCTTCAA GGCAACACCC AGATCTCTCG AAAGAAAGTT 
CTGCCTCTGC TCCAGGAAGC CCTGTCAGCG TATTTTGACT CCACGAACAT CCCTTTAGGA 
CAGCCTGAGA CAGAGGGTGT GTCAAGGGAA CAGGTGGACA AAGAATTGGA CAGGGCAGGC 
AACTCCCTGC TTTCTGGACT GAGTCAGGAT GAGGAGGAGC CTCCACTGCC CCCCACGCCC 
ATGAACAGCC TGGTGGACGA GTGCCCCCTG GATCAGGGGC TGCCGAAGTT CTCAGCCGAA 
GTCATCTTCG AGAAATGTAG TCAGATCTCG CTGTCAGAAC CTACCACTGC CTCCCTGTCT 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010801331.1
CDS161..1429
Translation

Target ORF information:

RefSeq Version XM_010803029.2
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010803029.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTGTCAG CCCTTGCCCT GGCTTTTTAC CTGGCAAAGA CAACTGAGGC GGAGGAAGTC 
TTTGTGCCAG TGTTAAATAT AAAACGTTCA GAGCTACCTC TACGAGGTGA CAATGTCTTC 
TTCCTTCAGA AGATTCACAT TCCAGAGTCC GTCTTGATTT TCCGGGATGA GATTGACCTC 
CATGCACTGC ACCAGGCTGG CCAGCTCACC CTCATCCTTG TTGACCATCA TGTCTTACCC 
AAAAGTGACG CAGCCCTAGA GGAGGCAGTG GCAGAGGTGC TAGACCATCG GCCCATCGAT 
CAGAGACACT GCCCTCCCTG CCATGTTTCA GTTGAACTGG TGGGGTCCTG CGCTACCCTG 
GTGGCCGAGA GAATCCTGCA GGGGGCACCA GAGATCTTGG ACAGGCAAAC TGCAGCCCTT 
CTTCATGGAA CAATCCTCCT GGACTGTGTG AACATGGACC TTAAGATTGG AAAGGCAACC 
CTCAAGGACA GCCACTATGT AGAGAAGCTG GAGGCCCTCT TCCCAGATCT GCCCAGCAGA 
AATGACATCT TTGATTCTCT GCAAAAGGCC AAGTTTGATG TATCAGGACT GACCACAGAG 
CAGATGCTGA GAAAGGACCA GAAGACCATC TCCAGACAAG GCACTAAGGT GGCCATTAGT 
GCAATATATA TGGATATGGA GGCTTTTCTG CAGAGGTCTG GCCTCCTTGC AGATCTCCGT 
GCCTTCTGCC AGGCTCATAG CTACGATGCC CTGGTTGCCA TGACTATCTT TTTCAACACT 
TACAATGAGC CAGTGCGGCA GTTGGCTGTT TTCTGTCCCC ACGCAGCACT TCGAATGACG 
ATCTGTGGAA TCCTGGAACA TTCCCACTCT CCACCCCTGA AGCTGACCCC TGTCCCAAGC 
AGCCACCCTG ACCTCCAAGC CTATCTTCAA GGCAACACCC AGATCTCTCG AAAGAAAGTT 
CTGCCTCTGC TCCAGGAAGC CCTGTCAGCG TATTTTGACT CCACGAACAT CCCTTTAGGA 
CAGCCTGAGA CAGAGGGTGT GTCAAGGGAA CAGGTGGACA AAGAATTGGA CAGGGCAGGC 
AACTCCCTGC TTTCTGGACT GAGTCAGGAT GAGGAGGAGC CTCCACTGCC CCCCACGCCC 
ATGAACAGCC TGGTGGACGA GTGCCCCCTG GATCAGGGGC TGCCGAAGTT CTCAGCCGAA 
GTCATCTTCG AGAAATGTAG TCAGATCTCG CTGTCAGAAC CTACCACTGC CTCCCTGTCT 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa50936
Clone ID Related Accession (Same CDS sequence) XM_010803029.3 , XM_010803029.2 , XM_024989371.1
Accession Version XM_024989371.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product exopolyphosphatase PRUNE1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTGTCAG CCCTTGCCCT GGCTTTTTAC CTGGCAAAGA CAACTGAGGC GGAGGAAGTC 
TTTGTGCCAG TGTTAAATAT AAAACGTTCA GAGCTACCTC TACGAGGTGA CAATGTCTTC 
TTCCTTCAGA AGATTCACAT TCCAGAGTCC GTCTTGATTT TCCGGGATGA GATTGACCTC 
CATGCACTGC ACCAGGCTGG CCAGCTCACC CTCATCCTTG TTGACCATCA TGTCTTACCC 
AAAAGTGACG CAGCCCTAGA GGAGGCAGTG GCAGAGGTGC TAGACCATCG GCCCATCGAT 
CAGAGACACT GCCCTCCCTG CCATGTTTCA GTTGAACTGG TGGGGTCCTG CGCTACCCTG 
GTGGCCGAGA GAATCCTGCA GGGGGCACCA GAGATCTTGG ACAGGCAAAC TGCAGCCCTT 
CTTCATGGAA CAATCCTCCT GGACTGTGTG AACATGGACC TTAAGATTGG AAAGGCAACC 
CTCAAGGACA GCCACTATGT AGAGAAGCTG GAGGCCCTCT TCCCAGATCT GCCCAGCAGA 
AATGACATCT TTGATTCTCT GCAAAAGGCC AAGTTTGATG TATCAGGACT GACCACAGAG 
CAGATGCTGA GAAAGGACCA GAAGACCATC TCCAGACAAG GCACTAAGGT GGCCATTAGT 
GCAATATATA TGGATATGGA GGCTTTTCTG CAGAGGTCTG GCCTCCTTGC AGATCTCCGT 
GCCTTCTGCC AGGCTCATAG CTACGATGCC CTGGTTGCCA TGACTATCTT TTTCAACACT 
TACAATGAGC CAGTGCGGCA GTTGGCTGTT TTCTGTCCCC ACGCAGCACT TCGAATGACG 
ATCTGTGGAA TCCTGGAACA TTCCCACTCT CCACCCCTGA AGCTGACCCC TGTCCCAAGC 
AGCCACCCTG ACCTCCAAGC CTATCTTCAA GGCAACACCC AGATCTCTCG AAAGAAAGTT 
CTGCCTCTGC TCCAGGAAGC CCTGTCAGCG TATTTTGACT CCACGAACAT CCCTTTAGGA 
CAGCCTGAGA CAGAGGGTGT GTCAAGGGAA CAGGTGGACA AAGAATTGGA CAGGGCAGGC 
AACTCCCTGC TTTCTGGACT GAGTCAGGAT GAGGAGGAGC CTCCACTGCC CCCCACGCCC 
ATGAACAGCC TGGTGGACGA GTGCCCCCTG GATCAGGGGC TGCCGAAGTT CTCAGCCGAA 
GTCATCTTCG AGAAATGTAG TCAGATCTCG CTGTCAGAAC CTACCACTGC CTCCCTGTCT 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024845139.1
CDS104..1372
Translation

Target ORF information:

RefSeq Version XM_024989371.1
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024989371.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTGTCAG CCCTTGCCCT GGCTTTTTAC CTGGCAAAGA CAACTGAGGC GGAGGAAGTC 
TTTGTGCCAG TGTTAAATAT AAAACGTTCA GAGCTACCTC TACGAGGTGA CAATGTCTTC 
TTCCTTCAGA AGATTCACAT TCCAGAGTCC GTCTTGATTT TCCGGGATGA GATTGACCTC 
CATGCACTGC ACCAGGCTGG CCAGCTCACC CTCATCCTTG TTGACCATCA TGTCTTACCC 
AAAAGTGACG CAGCCCTAGA GGAGGCAGTG GCAGAGGTGC TAGACCATCG GCCCATCGAT 
CAGAGACACT GCCCTCCCTG CCATGTTTCA GTTGAACTGG TGGGGTCCTG CGCTACCCTG 
GTGGCCGAGA GAATCCTGCA GGGGGCACCA GAGATCTTGG ACAGGCAAAC TGCAGCCCTT 
CTTCATGGAA CAATCCTCCT GGACTGTGTG AACATGGACC TTAAGATTGG AAAGGCAACC 
CTCAAGGACA GCCACTATGT AGAGAAGCTG GAGGCCCTCT TCCCAGATCT GCCCAGCAGA 
AATGACATCT TTGATTCTCT GCAAAAGGCC AAGTTTGATG TATCAGGACT GACCACAGAG 
CAGATGCTGA GAAAGGACCA GAAGACCATC TCCAGACAAG GCACTAAGGT GGCCATTAGT 
GCAATATATA TGGATATGGA GGCTTTTCTG CAGAGGTCTG GCCTCCTTGC AGATCTCCGT 
GCCTTCTGCC AGGCTCATAG CTACGATGCC CTGGTTGCCA TGACTATCTT TTTCAACACT 
TACAATGAGC CAGTGCGGCA GTTGGCTGTT TTCTGTCCCC ACGCAGCACT TCGAATGACG 
ATCTGTGGAA TCCTGGAACA TTCCCACTCT CCACCCCTGA AGCTGACCCC TGTCCCAAGC 
AGCCACCCTG ACCTCCAAGC CTATCTTCAA GGCAACACCC AGATCTCTCG AAAGAAAGTT 
CTGCCTCTGC TCCAGGAAGC CCTGTCAGCG TATTTTGACT CCACGAACAT CCCTTTAGGA 
CAGCCTGAGA CAGAGGGTGT GTCAAGGGAA CAGGTGGACA AAGAATTGGA CAGGGCAGGC 
AACTCCCTGC TTTCTGGACT GAGTCAGGAT GAGGAGGAGC CTCCACTGCC CCCCACGCCC 
ATGAACAGCC TGGTGGACGA GTGCCCCCTG GATCAGGGGC TGCCGAAGTT CTCAGCCGAA 
GTCATCTTCG AGAAATGTAG TCAGATCTCG CTGTCAGAAC CTACCACTGC CTCCCTGTCT 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa17429
Clone ID Related Accession (Same CDS sequence) XM_024989372.1
Accession Version XM_024989372.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1218bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product exopolyphosphatase PRUNE1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGATCCTG GGGTCCAGGA AAGACGAAGA TGTGGGATCT GGGGAGCACC GAAGGAGTCC 
CGACCCATAC ATGTTGTGCT GGGAAATGAA GCCTGTGACT TGGACTCCAT GGTGTCAGCC 
CTTGCCCTGG CTTTTTACCT GGCAAAGACA ACTGAGGCGG AGGAAGTCTT TGTGCCAGTG 
TTAAATATAA AACGTTCAGA GCTACCTCTA CGAGGTGACA ATGTCTTCTT CCTTCAGAAG 
ATTCACATTC CAGAGTCCGT CTTGATTTTC CGGGATGAGA TTGACCTCCA TGCACTGCAC 
CAGGCTGGCC AGCTCACCCT CATCCTTGTT GACCATCATG TCTTACCCAA AAGTGACGCA 
GCCCTAGAGG AGGCAGTGGC AGAGGTGCTA GACCATCGGC CCATCGATCA GAGACACTGC 
CCTCCCTGCC ATGTTTCAGT TGAACTGGTG GGGTCCTGCG CTACCCTGGT GGCCGAGAGA 
ATCCTGCAGG GGGCACCAGA GATCTTGGAC AGGCAAACTG CAGCCCTTCT TCATGGAACA 
ATCCTCCTGG ACTGTGTGAA CATGGACCTT AAGATTGGAA AGGCAACCCT CAAGGACAGC 
CACTATGTAG AGAAGCTGGA GGCCCTCTTC CCAGATCTGC CCAGCAGAAA TGACATCTTT 
GATTCTCTGC AAAAGGCCAA GTTTGATGTA TCAGGACTGA CCACAGAGCA GATGCTGAGA 
AAGGACCAGA AGACCATCTC CAGACAAGGC ACTAAGGTGG CCATTAGTGC AATATATATG 
GATATGGAGA TCTGTGGAAT CCTGGAACAT TCCCACTCTC CACCCCTGAA GCTGACCCCT 
GTCCCAAGCA GCCACCCTGA CCTCCAAGCC TATCTTCAAG GCAACACCCA GATCTCTCGA 
AAGAAAGTTC TGCCTCTGCT CCAGGAAGCC CTGTCAGCGT ATTTTGACTC CACGAACATC 
CCTTTAGGAC AGCCTGAGAC AGAGGGTGTG TCAAGGGAAC AGGTGGACAA AGAATTGGAC 
AGGGCAGGCA ACTCCCTGCT TTCTGGACTG AGTCAGGATG AGGAGGAGCC TCCACTGCCC 
CCCACGCCCA TGAACAGCCT GGTGGACGAG TGCCCCCTGG ATCAGGGGCT GCCGAAGTTC 
TCAGCCGAAG TCATCTTCGA GAAATGTAGT CAGATCTCGC TGTCAGAACC TACCACTGCC 
TCCCTGTCTA AGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024845140.1
CDS57..1274
Translation

Target ORF information:

RefSeq Version XM_024989372.1
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024989372.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGATCCTG GGGTCCAGGA AAGACGAAGA TGTGGGATCT GGGGAGCACC GAAGGAGTCC 
CGACCCATAC ATGTTGTGCT GGGAAATGAA GCCTGTGACT TGGACTCCAT GGTGTCAGCC 
CTTGCCCTGG CTTTTTACCT GGCAAAGACA ACTGAGGCGG AGGAAGTCTT TGTGCCAGTG 
TTAAATATAA AACGTTCAGA GCTACCTCTA CGAGGTGACA ATGTCTTCTT CCTTCAGAAG 
ATTCACATTC CAGAGTCCGT CTTGATTTTC CGGGATGAGA TTGACCTCCA TGCACTGCAC 
CAGGCTGGCC AGCTCACCCT CATCCTTGTT GACCATCATG TCTTACCCAA AAGTGACGCA 
GCCCTAGAGG AGGCAGTGGC AGAGGTGCTA GACCATCGGC CCATCGATCA GAGACACTGC 
CCTCCCTGCC ATGTTTCAGT TGAACTGGTG GGGTCCTGCG CTACCCTGGT GGCCGAGAGA 
ATCCTGCAGG GGGCACCAGA GATCTTGGAC AGGCAAACTG CAGCCCTTCT TCATGGAACA 
ATCCTCCTGG ACTGTGTGAA CATGGACCTT AAGATTGGAA AGGCAACCCT CAAGGACAGC 
CACTATGTAG AGAAGCTGGA GGCCCTCTTC CCAGATCTGC CCAGCAGAAA TGACATCTTT 
GATTCTCTGC AAAAGGCCAA GTTTGATGTA TCAGGACTGA CCACAGAGCA GATGCTGAGA 
AAGGACCAGA AGACCATCTC CAGACAAGGC ACTAAGGTGG CCATTAGTGC AATATATATG 
GATATGGAGA TCTGTGGAAT CCTGGAACAT TCCCACTCTC CACCCCTGAA GCTGACCCCT 
GTCCCAAGCA GCCACCCTGA CCTCCAAGCC TATCTTCAAG GCAACACCCA GATCTCTCGA 
AAGAAAGTTC TGCCTCTGCT CCAGGAAGCC CTGTCAGCGT ATTTTGACTC CACGAACATC 
CCTTTAGGAC AGCCTGAGAC AGAGGGTGTG TCAAGGGAAC AGGTGGACAA AGAATTGGAC 
AGGGCAGGCA ACTCCCTGCT TTCTGGACTG AGTCAGGATG AGGAGGAGCC TCCACTGCCC 
CCCACGCCCA TGAACAGCCT GGTGGACGAG TGCCCCCTGG ATCAGGGGCT GCCGAAGTTC 
TCAGCCGAAG TCATCTTCGA GAAATGTAGT CAGATCTCGC TGTCAGAACC TACCACTGCC 
TCCCTGTCTA AGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa90396
Clone ID Related Accession (Same CDS sequence) XM_015462708.2 , XM_015462708.1
Accession Version XM_015462708.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product exopolyphosphatase PRUNE1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015462708.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAGAACT ACCTGCAGGG TTGTCGAGCT GCTCTGCAGG AGTCCCGACC CATACATGTT 
GTGCTGGGAA ATGAAGCCTG TGACTTGGAC TCCATGGTGT CAGCCCTTGC CCTGGCTTTT 
TACCTGGCAA AGACAACTGA GGCGGAGGAA GTCTTTGTGC CAGTGTTAAA TATAAAACGT 
TCAGAGCTAC CTCTACGAGG TGACAATGTC TTCTTCCTTC AGAAGATTCA CATTCCAGAG 
TCCGTCTTGA TTTTCCGGGA TGAGATTGAC CTCCATGCAC TGCACCAGGC TGGCCAGCTC 
ACCCTCATCC TTGTTGACCA TCATGTCTTA CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA 
GTGGCAGAGG TGCTAGACCA TCGGCCCATC GATCAGAGAC ACTGCCCTCC CTGCCATGTT 
TCAGTTGAAC TGGTGGGGTC CTGCGCTACC CTGGTGGCCG AGAGAATCCT GCAGGGGGCA 
CCAGAGATCT TGGACAGGCA AACTGCAGCC CTTCTTCATG GAACAATCCT CCTGGACTGT 
GTGAACATGG ACCTTAAGAT TGGAAAGGCA ACCCTCAAGG ACAGCCACTA TGTAGAGAAG 
CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC AGAAATGACA TCTTTGATTC TCTGCAAAAG 
GCCAAGTTTG ATGTATCAGG ACTGACCACA GAGCAGATGC TGAGAAAGGA CCAGAAGACC 
ATCTCCAGAC AAGGCACTAA GGTGGCCATT AGTGCAATAT ATATGGATAT GGAGATCTGT 
GGAATCCTGG AACATTCCCA CTCTCCACCC CTGAAGCTGA CCCCTGTCCC AAGCAGCCAC 
CCTGACCTCC AAGCCTATCT TCAAGGCAAC ACCCAGATCT CTCGAAAGAA AGTTCTGCCT 
CTGCTCCAGG AAGCCCTGTC AGCGTATTTT GACTCCACGA ACATCCCTTT AGGACAGCCT 
GAGACAGAGG GTGTGTCAAG GGAACAGGTG GACAAAGAAT TGGACAGGGC AGGCAACTCC 
CTGCTTTCTG GACTGAGTCA GGATGAGGAG GAGCCTCCAC TGCCCCCCAC GCCCATGAAC 
AGCCTGGTGG ACGAGTGCCC CCTGGATCAG GGGCTGCCGA AGTTCTCAGC CGAAGTCATC 
TTCGAGAAAT GTAGTCAGAT CTCGCTGTCA GAACCTACCA CTGCCTCCCT GTCTAAGAAA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015318194.1
CDS311..1513
Translation

Target ORF information:

RefSeq Version XM_015462708.2
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015462708.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAGAACT ACCTGCAGGG TTGTCGAGCT GCTCTGCAGG AGTCCCGACC CATACATGTT 
GTGCTGGGAA ATGAAGCCTG TGACTTGGAC TCCATGGTGT CAGCCCTTGC CCTGGCTTTT 
TACCTGGCAA AGACAACTGA GGCGGAGGAA GTCTTTGTGC CAGTGTTAAA TATAAAACGT 
TCAGAGCTAC CTCTACGAGG TGACAATGTC TTCTTCCTTC AGAAGATTCA CATTCCAGAG 
TCCGTCTTGA TTTTCCGGGA TGAGATTGAC CTCCATGCAC TGCACCAGGC TGGCCAGCTC 
ACCCTCATCC TTGTTGACCA TCATGTCTTA CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA 
GTGGCAGAGG TGCTAGACCA TCGGCCCATC GATCAGAGAC ACTGCCCTCC CTGCCATGTT 
TCAGTTGAAC TGGTGGGGTC CTGCGCTACC CTGGTGGCCG AGAGAATCCT GCAGGGGGCA 
CCAGAGATCT TGGACAGGCA AACTGCAGCC CTTCTTCATG GAACAATCCT CCTGGACTGT 
GTGAACATGG ACCTTAAGAT TGGAAAGGCA ACCCTCAAGG ACAGCCACTA TGTAGAGAAG 
CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC AGAAATGACA TCTTTGATTC TCTGCAAAAG 
GCCAAGTTTG ATGTATCAGG ACTGACCACA GAGCAGATGC TGAGAAAGGA CCAGAAGACC 
ATCTCCAGAC AAGGCACTAA GGTGGCCATT AGTGCAATAT ATATGGATAT GGAGATCTGT 
GGAATCCTGG AACATTCCCA CTCTCCACCC CTGAAGCTGA CCCCTGTCCC AAGCAGCCAC 
CCTGACCTCC AAGCCTATCT TCAAGGCAAC ACCCAGATCT CTCGAAAGAA AGTTCTGCCT 
CTGCTCCAGG AAGCCCTGTC AGCGTATTTT GACTCCACGA ACATCCCTTT AGGACAGCCT 
GAGACAGAGG GTGTGTCAAG GGAACAGGTG GACAAAGAAT TGGACAGGGC AGGCAACTCC 
CTGCTTTCTG GACTGAGTCA GGATGAGGAG GAGCCTCCAC TGCCCCCCAC GCCCATGAAC 
AGCCTGGTGG ACGAGTGCCC CCTGGATCAG GGGCTGCCGA AGTTCTCAGC CGAAGTCATC 
TTCGAGAAAT GTAGTCAGAT CTCGCTGTCA GAACCTACCA CTGCCTCCCT GTCTAAGAAA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa90396
Clone ID Related Accession (Same CDS sequence) XM_015462708.2 , XM_015462708.1
Accession Version XM_015462708.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product protein prune homolog isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAGAACT ACCTGCAGGG TTGTCGAGCT GCTCTGCAGG AGTCCCGACC CATACATGTT 
GTGCTGGGAA ATGAAGCCTG TGACTTGGAC TCCATGGTGT CAGCCCTTGC CCTGGCTTTT 
TACCTGGCAA AGACAACTGA GGCGGAGGAA GTCTTTGTGC CAGTGTTAAA TATAAAACGT 
TCAGAGCTAC CTCTACGAGG TGACAATGTC TTCTTCCTTC AGAAGATTCA CATTCCAGAG 
TCCGTCTTGA TTTTCCGGGA TGAGATTGAC CTCCATGCAC TGCACCAGGC TGGCCAGCTC 
ACCCTCATCC TTGTTGACCA TCATGTCTTA CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA 
GTGGCAGAGG TGCTAGACCA TCGGCCCATC GATCAGAGAC ACTGCCCTCC CTGCCATGTT 
TCAGTTGAAC TGGTGGGGTC CTGCGCTACC CTGGTGGCCG AGAGAATCCT GCAGGGGGCA 
CCAGAGATCT TGGACAGGCA AACTGCAGCC CTTCTTCATG GAACAATCCT CCTGGACTGT 
GTGAACATGG ACCTTAAGAT TGGAAAGGCA ACCCTCAAGG ACAGCCACTA TGTAGAGAAG 
CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC AGAAATGACA TCTTTGATTC TCTGCAAAAG 
GCCAAGTTTG ATGTATCAGG ACTGACCACA GAGCAGATGC TGAGAAAGGA CCAGAAGACC 
ATCTCCAGAC AAGGCACTAA GGTGGCCATT AGTGCAATAT ATATGGATAT GGAGATCTGT 
GGAATCCTGG AACATTCCCA CTCTCCACCC CTGAAGCTGA CCCCTGTCCC AAGCAGCCAC 
CCTGACCTCC AAGCCTATCT TCAAGGCAAC ACCCAGATCT CTCGAAAGAA AGTTCTGCCT 
CTGCTCCAGG AAGCCCTGTC AGCGTATTTT GACTCCACGA ACATCCCTTT AGGACAGCCT 
GAGACAGAGG GTGTGTCAAG GGAACAGGTG GACAAAGAAT TGGACAGGGC AGGCAACTCC 
CTGCTTTCTG GACTGAGTCA GGATGAGGAG GAGCCTCCAC TGCCCCCCAC GCCCATGAAC 
AGCCTGGTGG ACGAGTGCCC CCTGGATCAG GGGCTGCCGA AGTTCTCAGC CGAAGTCATC 
TTCGAGAAAT GTAGTCAGAT CTCGCTGTCA GAACCTACCA CTGCCTCCCT GTCTAAGAAA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015318194.1
CDS1042..2244
Translation

Target ORF information:

RefSeq Version XM_015462708.1
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015462708.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAGAACT ACCTGCAGGG TTGTCGAGCT GCTCTGCAGG AGTCCCGACC CATACATGTT 
GTGCTGGGAA ATGAAGCCTG TGACTTGGAC TCCATGGTGT CAGCCCTTGC CCTGGCTTTT 
TACCTGGCAA AGACAACTGA GGCGGAGGAA GTCTTTGTGC CAGTGTTAAA TATAAAACGT 
TCAGAGCTAC CTCTACGAGG TGACAATGTC TTCTTCCTTC AGAAGATTCA CATTCCAGAG 
TCCGTCTTGA TTTTCCGGGA TGAGATTGAC CTCCATGCAC TGCACCAGGC TGGCCAGCTC 
ACCCTCATCC TTGTTGACCA TCATGTCTTA CCCAAAAGTG ACGCAGCCCT AGAGGAGGCA 
GTGGCAGAGG TGCTAGACCA TCGGCCCATC GATCAGAGAC ACTGCCCTCC CTGCCATGTT 
TCAGTTGAAC TGGTGGGGTC CTGCGCTACC CTGGTGGCCG AGAGAATCCT GCAGGGGGCA 
CCAGAGATCT TGGACAGGCA AACTGCAGCC CTTCTTCATG GAACAATCCT CCTGGACTGT 
GTGAACATGG ACCTTAAGAT TGGAAAGGCA ACCCTCAAGG ACAGCCACTA TGTAGAGAAG 
CTGGAGGCCC TCTTCCCAGA TCTGCCCAGC AGAAATGACA TCTTTGATTC TCTGCAAAAG 
GCCAAGTTTG ATGTATCAGG ACTGACCACA GAGCAGATGC TGAGAAAGGA CCAGAAGACC 
ATCTCCAGAC AAGGCACTAA GGTGGCCATT AGTGCAATAT ATATGGATAT GGAGATCTGT 
GGAATCCTGG AACATTCCCA CTCTCCACCC CTGAAGCTGA CCCCTGTCCC AAGCAGCCAC 
CCTGACCTCC AAGCCTATCT TCAAGGCAAC ACCCAGATCT CTCGAAAGAA AGTTCTGCCT 
CTGCTCCAGG AAGCCCTGTC AGCGTATTTT GACTCCACGA ACATCCCTTT AGGACAGCCT 
GAGACAGAGG GTGTGTCAAG GGAACAGGTG GACAAAGAAT TGGACAGGGC AGGCAACTCC 
CTGCTTTCTG GACTGAGTCA GGATGAGGAG GAGCCTCCAC TGCCCCCCAC GCCCATGAAC 
AGCCTGGTGG ACGAGTGCCC CCTGGATCAG GGGCTGCCGA AGTTCTCAGC CGAAGTCATC 
TTCGAGAAAT GTAGTCAGAT CTCGCTGTCA GAACCTACCA CTGCCTCCCT GTCTAAGAAA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa90397
Clone ID Related Accession (Same CDS sequence) XM_015462709.2 , XM_015462709.1
Accession Version XM_015462709.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 918bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product exopolyphosphatase PRUNE1 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015462709.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGATTGA CCTCCATGCA CTGCACCAGG CTGGCCAGCT CACCCTCATC CTTGTTGACC 
ATCATGTCTT ACCCAAGAAC AATCCTCCTG GACTGTGTGA ACATGGACCT TAAGATTGGA 
AAGGCAACCC TCAAGGACAG CCACTATGTA GAGAAGCTGG AGGCCCTCTT CCCAGATCTG 
CCCAGCAGAA ATGACATCTT TGATTCTCTG CAAAAGGCCA AGTTTGATGT ATCAGGACTG 
ACCACAGAGC AGATGCTGAG AAAGGACCAG AAGACCATCT CCAGACAAGG CACTAAGGTG 
GCCATTAGTG CAATATATAT GGATATGGAG GCTTTTCTGC AGAGGTCTGG CCTCCTTGCA 
GATCTCCGTG CCTTCTGCCA GGCTCATAGC TACGATGCCC TGGTTGCCAT GACTATCTTT 
TTCAACACTT ACAATGAGCC AGTGCGGCAG TTGGCTGTTT TCTGTCCCCA CGCAGCACTT 
CGAATGACGA TCTGTGGAAT CCTGGAACAT TCCCACTCTC CACCCCTGAA GCTGACCCCT 
GTCCCAAGCA GCCACCCTGA CCTCCAAGCC TATCTTCAAG GCAACACCCA GATCTCTCGA 
AAGAAAGTTC TGCCTCTGCT CCAGGAAGCC CTGTCAGCGT ATTTTGACTC CACGAACATC 
CCTTTAGGAC AGCCTGAGAC AGAGGGTGTG TCAAGGGAAC AGGTGGACAA AGAATTGGAC 
AGGGCAGGCA ACTCCCTGCT TTCTGGACTG AGTCAGGATG AGGAGGAGCC TCCACTGCCC 
CCCACGCCCA TGAACAGCCT GGTGGACGAG TGCCCCCTGG ATCAGGGGCT GCCGAAGTTC 
TCAGCCGAAG TCATCTTCGA GAAATGTAGT CAGATCTCGC TGTCAGAACC TACCACTGCC 
TCCCTGTCTA AGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015318195.1
CDS566..1483
Translation

Target ORF information:

RefSeq Version XM_015462709.2
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase 1 (PRUNE1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015462709.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGATTGA CCTCCATGCA CTGCACCAGG CTGGCCAGCT CACCCTCATC CTTGTTGACC 
ATCATGTCTT ACCCAAGAAC AATCCTCCTG GACTGTGTGA ACATGGACCT TAAGATTGGA 
AAGGCAACCC TCAAGGACAG CCACTATGTA GAGAAGCTGG AGGCCCTCTT CCCAGATCTG 
CCCAGCAGAA ATGACATCTT TGATTCTCTG CAAAAGGCCA AGTTTGATGT ATCAGGACTG 
ACCACAGAGC AGATGCTGAG AAAGGACCAG AAGACCATCT CCAGACAAGG CACTAAGGTG 
GCCATTAGTG CAATATATAT GGATATGGAG GCTTTTCTGC AGAGGTCTGG CCTCCTTGCA 
GATCTCCGTG CCTTCTGCCA GGCTCATAGC TACGATGCCC TGGTTGCCAT GACTATCTTT 
TTCAACACTT ACAATGAGCC AGTGCGGCAG TTGGCTGTTT TCTGTCCCCA CGCAGCACTT 
CGAATGACGA TCTGTGGAAT CCTGGAACAT TCCCACTCTC CACCCCTGAA GCTGACCCCT 
GTCCCAAGCA GCCACCCTGA CCTCCAAGCC TATCTTCAAG GCAACACCCA GATCTCTCGA 
AAGAAAGTTC TGCCTCTGCT CCAGGAAGCC CTGTCAGCGT ATTTTGACTC CACGAACATC 
CCTTTAGGAC AGCCTGAGAC AGAGGGTGTG TCAAGGGAAC AGGTGGACAA AGAATTGGAC 
AGGGCAGGCA ACTCCCTGCT TTCTGGACTG AGTCAGGATG AGGAGGAGCC TCCACTGCCC 
CCCACGCCCA TGAACAGCCT GGTGGACGAG TGCCCCCTGG ATCAGGGGCT GCCGAAGTTC 
TCAGCCGAAG TCATCTTCGA GAAATGTAGT CAGATCTCGC TGTCAGAACC TACCACTGCC 
TCCCTGTCTA AGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa90397
Clone ID Related Accession (Same CDS sequence) XM_015462709.2 , XM_015462709.1
Accession Version XM_015462709.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 918bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product protein prune homolog isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGATTGA CCTCCATGCA CTGCACCAGG CTGGCCAGCT CACCCTCATC CTTGTTGACC 
ATCATGTCTT ACCCAAGAAC AATCCTCCTG GACTGTGTGA ACATGGACCT TAAGATTGGA 
AAGGCAACCC TCAAGGACAG CCACTATGTA GAGAAGCTGG AGGCCCTCTT CCCAGATCTG 
CCCAGCAGAA ATGACATCTT TGATTCTCTG CAAAAGGCCA AGTTTGATGT ATCAGGACTG 
ACCACAGAGC AGATGCTGAG AAAGGACCAG AAGACCATCT CCAGACAAGG CACTAAGGTG 
GCCATTAGTG CAATATATAT GGATATGGAG GCTTTTCTGC AGAGGTCTGG CCTCCTTGCA 
GATCTCCGTG CCTTCTGCCA GGCTCATAGC TACGATGCCC TGGTTGCCAT GACTATCTTT 
TTCAACACTT ACAATGAGCC AGTGCGGCAG TTGGCTGTTT TCTGTCCCCA CGCAGCACTT 
CGAATGACGA TCTGTGGAAT CCTGGAACAT TCCCACTCTC CACCCCTGAA GCTGACCCCT 
GTCCCAAGCA GCCACCCTGA CCTCCAAGCC TATCTTCAAG GCAACACCCA GATCTCTCGA 
AAGAAAGTTC TGCCTCTGCT CCAGGAAGCC CTGTCAGCGT ATTTTGACTC CACGAACATC 
CCTTTAGGAC AGCCTGAGAC AGAGGGTGTG TCAAGGGAAC AGGTGGACAA AGAATTGGAC 
AGGGCAGGCA ACTCCCTGCT TTCTGGACTG AGTCAGGATG AGGAGGAGCC TCCACTGCCC 
CCCACGCCCA TGAACAGCCT GGTGGACGAG TGCCCCCTGG ATCAGGGGCT GCCGAAGTTC 
TCAGCCGAAG TCATCTTCGA GAAATGTAGT CAGATCTCGC TGTCAGAACC TACCACTGCC 
TCCCTGTCTA AGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015318195.1
CDS1294..2211
Translation

Target ORF information:

RefSeq Version XM_015462709.1
Organism Bos taurus(cattle)
Definition Bos taurus prune exopolyphosphatase (PRUNE), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015462709.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGATTGA CCTCCATGCA CTGCACCAGG CTGGCCAGCT CACCCTCATC CTTGTTGACC 
ATCATGTCTT ACCCAAGAAC AATCCTCCTG GACTGTGTGA ACATGGACCT TAAGATTGGA 
AAGGCAACCC TCAAGGACAG CCACTATGTA GAGAAGCTGG AGGCCCTCTT CCCAGATCTG 
CCCAGCAGAA ATGACATCTT TGATTCTCTG CAAAAGGCCA AGTTTGATGT ATCAGGACTG 
ACCACAGAGC AGATGCTGAG AAAGGACCAG AAGACCATCT CCAGACAAGG CACTAAGGTG 
GCCATTAGTG CAATATATAT GGATATGGAG GCTTTTCTGC AGAGGTCTGG CCTCCTTGCA 
GATCTCCGTG CCTTCTGCCA GGCTCATAGC TACGATGCCC TGGTTGCCAT GACTATCTTT 
TTCAACACTT ACAATGAGCC AGTGCGGCAG TTGGCTGTTT TCTGTCCCCA CGCAGCACTT 
CGAATGACGA TCTGTGGAAT CCTGGAACAT TCCCACTCTC CACCCCTGAA GCTGACCCCT 
GTCCCAAGCA GCCACCCTGA CCTCCAAGCC TATCTTCAAG GCAACACCCA GATCTCTCGA 
AAGAAAGTTC TGCCTCTGCT CCAGGAAGCC CTGTCAGCGT ATTTTGACTC CACGAACATC 
CCTTTAGGAC AGCCTGAGAC AGAGGGTGTG TCAAGGGAAC AGGTGGACAA AGAATTGGAC 
AGGGCAGGCA ACTCCCTGCT TTCTGGACTG AGTCAGGATG AGGAGGAGCC TCCACTGCCC 
CCCACGCCCA TGAACAGCCT GGTGGACGAG TGCCCCCTGG ATCAGGGGCT GCCGAAGTTC 
TCAGCCGAAG TCATCTTCGA GAAATGTAGT CAGATCTCGC TGTCAGAACC TACCACTGCC 
TCCCTGTCTA AGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL


book

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP


book

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW