HACE1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following HACE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HACE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa00714 NM_001192645.2
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22599 XM_005210841.3
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22600 XM_005210842.3
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22601 XM_005210843.4
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa51409 XM_010808467.3
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa237783 XM_024996667.1
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa237784 XM_024996668.1
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa237785 XM_024996669.1
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa237786 XM_024996670.1
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa237787 XM_024996671.1
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa237788 XM_024996672.1
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa237789 XM_024996673.1
Latest version!
Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OBa22601 XM_005210843.3 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa51409 XM_010808467.2 Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa00714
    Clone ID Related Accession (Same CDS sequence) NM_001192645.2
    Accession Version NM_001192645.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-20
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH179641.1 and NKLS02000009.1. On Apr 22, 2011 this sequence version replaced NM_001192645.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571263.4530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG
    GTCCCACATG GTGGATTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTCACAATT
    GCTGCTGTGC CATATACTCC AAATCTTCTA CCTACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG CAAAGAAATA CTCAAGGACA GGCTTCTTGT GGCACTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179574.2
    CDS180..2909
    Translation

    Target ORF information:

    RefSeq Version NM_001192645.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192645.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG
    GTCCCACATG GTGGATTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTCACAATT
    GCTGCTGTGC CATATACTCC AAATCTTCTA CCTACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG CAAAGAAATA CTCAAGGACA GGCTTCTTGT GGCACTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22599
    Clone ID Related Accession (Same CDS sequence) XM_005210841.3
    Accession Version XM_005210841.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210841.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT
    CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA
    AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA
    GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT
    GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG
    GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT
    AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA
    ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG
    TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA
    AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG
    ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT
    AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA
    ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG
    CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT
    ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT
    CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG
    CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC
    CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA
    ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT
    GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG
    TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT
    GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG
    TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA
    GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT
    CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC
    TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA
    GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT
    CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC
    TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT
    GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT
    CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA
    AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA
    TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA
    ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT
    CTCTTGCAGT TTGTTACTGG CAGTTCCAGG GTCCCACATG GTGGATTTGC TAATATCATG
    GGTGGAAGTG GATTGCAAAA CTTCACAATT GCTGCTGTGC CATATACTCC AAATCTTCTA
    CCTACTTCAA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG CAAAGAAATA
    CTCAAGGACA GGCTTCTTGT GGCACTACAT TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210898.1
    CDS240..2879
    Translation

    Target ORF information:

    RefSeq Version XM_005210841.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210841.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT
    CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA
    AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA
    GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT
    GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG
    GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT
    AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA
    ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG
    TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA
    AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG
    ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT
    AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA
    ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG
    CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT
    ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT
    CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG
    CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC
    CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA
    ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT
    GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG
    TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT
    GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG
    TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA
    GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT
    CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC
    TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA
    GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT
    CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC
    TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT
    GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT
    CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA
    AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA
    TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA
    ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT
    CTCTTGCAGT TTGTTACTGG CAGTTCCAGG GTCCCACATG GTGGATTTGC TAATATCATG
    GGTGGAAGTG GATTGCAAAA CTTCACAATT GCTGCTGTGC CATATACTCC AAATCTTCTA
    CCTACTTCAA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG CAAAGAAATA
    CTCAAGGACA GGCTTCTTGT GGCACTACAT TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22600
    Clone ID Related Accession (Same CDS sequence) XM_005210842.3
    Accession Version XM_005210842.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG
    AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA
    ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC
    AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT
    GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT
    GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG
    ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG
    CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT
    CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC
    CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA
    GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA
    CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA
    CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT
    CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA
    TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT
    CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT
    AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT
    GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT
    TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA
    AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA
    CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT
    GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TCACAATTGC TGCTGTGCCA TATACTCCAA ATCTTCTACC TACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGCA AAGAAATACT CAAGGACAGG
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210899.1
    CDS149..2776
    Translation

    Target ORF information:

    RefSeq Version XM_005210842.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210842.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG
    AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA
    ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC
    AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT
    GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT
    GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG
    ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG
    CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT
    CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC
    CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA
    GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA
    CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA
    CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT
    CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA
    TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT
    CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT
    AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT
    GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT
    TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA
    AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA
    CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT
    GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TCACAATTGC TGCTGTGCCA TATACTCCAA ATCTTCTACC TACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGCA AAGAAATACT CAAGGACAGG
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22601
    Clone ID Related Accession (Same CDS sequence) XM_005210843.3 , XM_005210843.4
    Accession Version XM_005210843.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG
    GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG
    TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT
    AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG
    GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC
    CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA
    TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA
    GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC
    ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210900.1
    CDS240..2837
    Translation

    Target ORF information:

    RefSeq Version XM_005210843.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210843.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG
    GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG
    TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT
    AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG
    GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC
    CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA
    TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA
    GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC
    ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22601
    Clone ID Related Accession (Same CDS sequence) XM_005210843.3 , XM_005210843.4
    Accession Version XM_005210843.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210843.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG
    GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG
    TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT
    AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG
    GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC
    CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA
    TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA
    GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC
    ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210900.1
    CDS239..2836
    Translation

    Target ORF information:

    RefSeq Version XM_005210843.4
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210843.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG
    GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG
    TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT
    AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG
    GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC
    CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA
    TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA
    GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC
    ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TCACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTTCTACC TACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGCA AAGAAATACT CAAGGACAGG CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51409
    Clone ID Related Accession (Same CDS sequence) XM_010808467.2 , XM_010808467.3
    Accession Version XM_010808467.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806769.1
    CDS242..2857
    Translation

    Target ORF information:

    RefSeq Version XM_010808467.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808467.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51409
    Clone ID Related Accession (Same CDS sequence) XM_010808467.2 , XM_010808467.3
    Accession Version XM_010808467.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808467.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806769.1
    CDS239..2854
    Translation

    Target ORF information:

    RefSeq Version XM_010808467.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808467.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237783
    Clone ID Related Accession (Same CDS sequence) XM_024996667.1
    Accession Version XM_024996667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCCGTGAA
    GAGATACCTC ACGTCCAAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT ATCATGGGTG
    GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTCTACCTA
    CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA
    AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG GCATAATGAA
    GTCTGGAAAA TTCTTCTGAC TGCTGATGAG CAATTCAGAG TGGCAGAAGT AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852435.1
    CDS251..3067
    Translation

    Target ORF information:

    RefSeq Version XM_024996667.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAATACACTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTACAACACG TCAGTGATGT CGATGTTGAG
    GATGCGATGG GGCAGACAGC GCTGCATGTG GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAATGCTTGC TGGACAGTGG TGCTGATATT AACAGACCAA ATGTATCAGG AGCCACTCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AGATTCTTCT ATTACGAGGT
    GCCAAGTATC TGTCAGATAA AAACGGAGTA ACCCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CCTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTATTTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGTC ATAAATTGCT TAGCATATCT AGTAATTACG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTGCTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAG GGGAATCTAA ACCAGATGTG CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACTTCGC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAACAGGA ATCCCAAGAT TATATTTGAC
    CACTTTCACT TCCTTCTCGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAG
    GCACAGCCAT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCATAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT CCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAACC CAATAGCAAC TCCTCTGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTCTGAACC ATAGGCAACT GGTGAACATT
    TACTTCACGA GATCATTCTA TAAGCACATT CTTGGTATTC CTGTGAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAC TTGCAATGGA TTTTAGATAA TGATATTAGT
    GATTTGGGTC TAGAATTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCCTTGA AACCTGGGGG TGGAAGTATT CTTGTAACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTTG TCACTGAACT GCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCA TCCCTCATAC AACTTTTTGA TGAATACGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGATATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGCCGTGAA
    GAGATACCTC ACGTCCAAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT ATCATGGGTG
    GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTCTACCTA
    CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA GAAATACTCA
    AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG GCATAATGAA
    GTCTGGAAAA TTCTTCTGAC TGCTGATGAG CAATTCAGAG TGGCAGAAGT AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237784
    Clone ID Related Accession (Same CDS sequence) XM_024996668.1
    Accession Version XM_024996668.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT
    CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA
    AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA
    GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT
    GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG
    GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT
    AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA
    ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG
    TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA
    AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG
    ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT
    AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA
    ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG
    CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT
    ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT
    CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG
    CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC
    CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA
    ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT
    GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG
    TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT
    GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG
    TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA
    GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT
    CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC
    TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA
    GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT
    CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC
    TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT
    GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT
    CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA
    AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA
    TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA
    ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT
    CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC
    CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG
    CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT
    TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG
    GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG
    CAATTCAGAG TGGCAGAAGT AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852436.1
    CDS251..2977
    Translation

    Target ORF information:

    RefSeq Version XM_024996668.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996668.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GTTGTGGATC AGTGGAATGC TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT
    CCTAACTATC AAGATATTTC GGGCTGTACA CCTCTTCATT TGGCAGCTAG AAATGGGCAA
    AAGAAATGCA TGAGTAAATT ATTAGAATAC AGTGCTGATG TCAACATTTG TAATAATGAA
    GGTCTTACTG CAATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT
    GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG
    GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT
    AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA
    ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG
    TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA
    AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG
    ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT
    AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA
    ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG
    CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT
    ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT
    CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG
    CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC
    CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA
    ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT
    GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG
    TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT
    GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG
    TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA
    GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT
    CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC
    TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA
    GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT
    CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC
    TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT
    GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT
    CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA
    AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA
    TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA
    ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT
    CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC
    CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG
    CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT
    TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG
    GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG
    CAATTCAGAG TGGCAGAAGT AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237785
    Clone ID Related Accession (Same CDS sequence) XM_024996669.1
    Accession Version XM_024996669.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG
    AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA
    ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC
    AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT
    GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT
    GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG
    ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG
    CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT
    CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC
    CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA
    GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA
    CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA
    CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT
    CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA
    TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT
    CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT
    AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT
    GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT
    TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA
    AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA
    CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT
    GTTACTGGCA GCCGTGAAGA GATACCTCAC GTCCAAGTTC CAGGGTCCCA CATGGTGGAT
    TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTCAC AATTGCTGCT GTGCCATATA
    CTCCAAATCT TCTACCTACT TCAAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC
    CAAGCAAAGA AATACTCAAG GACAGGCTTC TTGTGGCACT ACATTGTGGC AGCTATGGTT
    ACACAATGGC ATAATGAAGT CTGGAAAATT CTTCTGACTG CTGATGAGCA ATTCAGAGTG
    GCAGAAGTAA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852437.1
    CDS499..3213
    Translation

    Target ORF information:

    RefSeq Version XM_024996669.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996669.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGGAACC CTTCAGCACC AGTTTATAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCAG TGGAATGCTT GGTTCTGCTT TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCGG GCTGTACACC TCTTCATTTG GCAGCTAGAA ATGGGCAAAA GAAATGCATG
    AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG TCTTACTGCA
    ATACACTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT ACAACACGTC
    AGTGATGTCG ATGTTGAGGA TGCGATGGGG CAGACAGCGC TGCATGTGGC CTGCCAGAAT
    GGTCACAAGA CGACAGTGCA ATGCTTGCTG GACAGTGGTG CTGATATTAA CAGACCAAAT
    GTATCAGGAG CCACTCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAG
    ATTCTTCTAT TACGAGGTGC CAAGTATCTG TCAGATAAAA ACGGAGTAAC CCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACC TGTGAAGTAT TAATTCAGTA TCACCCTAGG
    CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ATTTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCCACAAC AAATGGTCAT AAATTGCTTA GCATATCTAG TAATTACGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT
    CCATCCTCTC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGGAGC
    CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA
    GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTGCTGAAA
    CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAGGG GAATCTAAAC CAGATGTGCT TGGAGGGAAA
    CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACTTCGCCC
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAACAGGAAT
    CCCAAGATTA TATTTGACCA CTTTCACTTC CTTCTCGAAT GTCCTGAATT GATGTCAAGA
    TTCATGCATA TCATAAAGGC ACAGCCATTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGACATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ATAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTCC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAACCCA ATAGCAACTC CTCTGTAAAT
    CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TCTGAACCAT
    AGGCAACTGG TGAACATTTA CTTCACGAGA TCATTCTATA AGCACATTCT TGGTATTCCT
    GTGAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAACTT GCAATGGATT
    TTAGATAATG ATATTAGTGA TTTGGGTCTA GAATTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA CCTGGGGGTG GAAGTATTCT TGTAACACAA
    AATAACAAAG CGGAGTACGT CCAGCTTGTC ACTGAACTGC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCATC CCTCATACAA
    CTTTTTGATG AATACGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTGGA AGATATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT
    GTTACTGGCA GCCGTGAAGA GATACCTCAC GTCCAAGTTC CAGGGTCCCA CATGGTGGAT
    TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTCAC AATTGCTGCT GTGCCATATA
    CTCCAAATCT TCTACCTACT TCAAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC
    CAAGCAAAGA AATACTCAAG GACAGGCTTC TTGTGGCACT ACATTGTGGC AGCTATGGTT
    ACACAATGGC ATAATGAAGT CTGGAAAATT CTTCTGACTG CTGATGAGCA ATTCAGAGTG
    GCAGAAGTAA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237786
    Clone ID Related Accession (Same CDS sequence) XM_024996670.1
    Accession Version XM_024996670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG
    GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG
    TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT
    AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG
    GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC
    CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA
    TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA
    GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC
    ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GCCGTGAAGA GATACCTCAC
    GTCCAAGTTC CAGGGTCCCA CATGGTGGAT TTGCTAATAT CATGGGTGGA AGTGGATTGC
    AAAACTTCAC AATTGCTGCT GTGCCATATA CTCCAAATCT TCTACCTACT TCAAGCACAT
    GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGCAAAGA AATACTCAAG GACAGGCTTC
    TTGTGGCACT ACATTGTGGC AGCTATGGTT ACACAATGGC ATAATGAAGT CTGGAAAATT
    CTTCTGACTG CTGATGAGCA ATTCAGAGTG GCAGAAGTAA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852438.1
    CDS239..2923
    Translation

    Target ORF information:

    RefSeq Version XM_024996670.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGAGAG CGATGGAGCA GCTCAATCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGATGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTTCTGC TTTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC GGGCTGTACA
    CCTCTTCATT TGGCAGCTAG AAATGGGCAA AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGTCTTACTG CAACAGTGCA ATGCTTGCTG
    GACAGTGGTG CTGATATTAA CAGACCAAAT GTATCAGGAG CCACTCCACT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAG ATTCTTCTAT TACGAGGTGC CAAGTATCTG
    TCAGATAAAA ACGGAGTAAC CCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACC
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ATTTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGTCAT
    AAATTGCTTA GCATATCTAG TAATTACGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGGAGC CAGGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTGCTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAGGG
    GAATCTAAAC CAGATGTGCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACTTCGCCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAACAGGAAT CCCAAGATTA TATTTGACCA CTTTCACTTC
    CTTCTCGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA TCATAAAGGC ACAGCCATTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ATAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTCC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAACCCA ATAGCAACTC CTCTGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TCTGAACCAT AGGCAACTGG TGAACATTTA CTTCACGAGA
    TCATTCTATA AGCACATTCT TGGTATTCCT GTGAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAACTT GCAATGGATT TTAGATAATG ATATTAGTGA TTTGGGTCTA
    GAATTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCCTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTAACACAA AATAACAAAG CGGAGTACGT CCAGCTTGTC
    ACTGAACTGC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCATC CCTCATACAA CTTTTTGATG AATACGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGATATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GCCGTGAAGA GATACCTCAC
    GTCCAAGTTC CAGGGTCCCA CATGGTGGAT TTGCTAATAT CATGGGTGGA AGTGGATTGC
    AAAACTTCAC AATTGCTGCT GTGCCATATA CTCCAAATCT TCTACCTACT TCAAGCACAT
    GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGCAAAGA AATACTCAAG GACAGGCTTC
    TTGTGGCACT ACATTGTGGC AGCTATGGTT ACACAATGGC ATAATGAAGT CTGGAAAATT
    CTTCTGACTG CTGATGAGCA ATTCAGAGTG GCAGAAGTAA TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237787
    Clone ID Related Accession (Same CDS sequence) XM_024996671.1
    Accession Version XM_024996671.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTAC AGATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
    GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG
    GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT
    AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA
    ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG
    TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA
    AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG
    ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT
    AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA
    ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG
    CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT
    ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT
    CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG
    CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC
    CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA
    ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT
    GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG
    TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT
    GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG
    TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA
    GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT
    CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC
    TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA
    GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT
    CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC
    TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT
    GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT
    CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA
    AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA
    TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA
    ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT
    CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC
    CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG
    CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT
    TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG
    GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG
    CAATTCAGAG TGGCAGAAGT AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852439.1
    CDS85..2511
    Translation

    Target ORF information:

    RefSeq Version XM_024996671.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996671.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCTGTAC AGATACACTG GCTGGCTGTG AATGGGCGGA CAGAACTACT CCATGACCTT 
    GTACAACACG TCAGTGATGT CGATGTTGAG GATGCGATGG GGCAGACAGC GCTGCATGTG
    GCCTGCCAGA ATGGTCACAA GACGACAGTG CAATGCTTGC TGGACAGTGG TGCTGATATT
    AACAGACCAA ATGTATCAGG AGCCACTCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GACACAGCAC AGATTCTTCT ATTACGAGGT GCCAAGTATC TGTCAGATAA AAACGGAGTA
    ACCCCTCTGG ATTTATGTGT ACAGGGTGGA TATGGAGAGA CCTGTGAAGT ATTAATTCAG
    TATCACCCTA GGCTTTTCCA GACAATTATT CAAATGACAC AGAATGAAGA TCTCCGAGAA
    AACATGTTAC GGCAAGTTCT GGAGCATTTG TCTCAGCAAA GTGAAAGCCA GTATTTAAAG
    ATTCTAACAA GCCTTGCTGA AGTTGCCACA ACAAATGGTC ATAAATTGCT TAGCATATCT
    AGTAATTACG ATGCTCAAAT GAAGAGCCTT TTAAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCATCCTC TCCCAGTAAT GGAATTGATA TGGGCTACAA TGGGAATAAA
    ACTCCAAGGA GCCAGGTGTT CAAGCCTCTT GAATTGCTTT GGCACTCATT AGATGAATGG
    CTAGTTTTAA TAGCTACAGA ATTGATGAAA AACAAAAAGG ACTCAACGGA TATCACTTCT
    ATTTTGCTGA AACAAAAAGG CCAAGATCAA GATGGTACTT CCATTCCTCC ATTTGAACCT
    CCAGGACCTG GGAGCTATGA AAATCTGTCC ACTGGCACAG GGGAATCTAA ACCAGATGTG
    CTTGGAGGGA AACAGGAAGC CAGTGCAGAC TGTCAGGATG TTATTTCTAT GACAGCTAAC
    CGGCTAAGTG CTGTCATTCA AGCTTTTTAC ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA
    ATGACTTCGC CCCGTTTCAT TGAATTTGTC TGCAAACATG ATGAAGTTTT AAAATGCTTT
    GTTAACAGGA ATCCCAAGAT TATATTTGAC CACTTTCACT TCCTTCTCGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAG GCACAGCCAT TTAAAGATCG CTGTGAATGG
    TTCTATGAAC ATTTGCATTC AGGACAGCCA GATTCAGACA TGGTGCACAG GCCAGTGAAT
    GAAAATGATA TCCTGCTGGT TCATAGAGAT TCTATTTTTA GGAGTAGCTG TGAAGTTGTG
    TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA GGAATTGCTG TACGGTTCCA TGGAGAAGAA
    GGCATGGGTC AAGGTGTTGT CCGTGAGTGG TTTGATATTC TGTCTAATGA GATAGTCAAT
    CCTGATTATG CATTGTTTAC CCAGTCAGCT GATGGAACAA CTTTTCAACC CAATAGCAAC
    TCCTCTGTAA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGATTA
    GCTCTGAACC ATAGGCAACT GGTGAACATT TACTTCACGA GATCATTCTA TAAGCACATT
    CTTGGTATTC CTGTGAATTA CCAAGATGTG GCATCCATTG ATCCAGAATA TGCCAAAAAC
    TTGCAATGGA TTTTAGATAA TGATATTAGT GATTTGGGTC TAGAATTAAC TTTTTCTGTT
    GAGACTGATG TGTTTGGAGC AATGGAAGAG GTGCCCTTGA AACCTGGGGG TGGAAGTATT
    CTTGTAACAC AAAATAACAA AGCGGAGTAC GTCCAGCTTG TCACTGAACT GCGAATGACA
    AGAGCCATTC AGCCTCAGAT CAATGCTTTT TTACAGGGCT TTCATATGTT CATTCCACCA
    TCCCTCATAC AACTTTTTGA TGAATACGAA TTGGAGCTAC TGCTTTCTGG CATGCCAGAA
    ATTGATGTGA GTGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGATATTA CTCCAGAGGA GAGAGTTCTT
    CTCTTGCAGT TTGTTACTGG CAGCCGTGAA GAGATACCTC ACGTCCAAGT TCCAGGGTCC
    CACATGGTGG ATTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG
    CTGTGCCATA TACTCCAAAT CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT
    TACCTGAATA CCCAAGCAAA GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG
    GCAGCTATGG TTACACAATG GCATAATGAA GTCTGGAAAA TTCTTCTGAC TGCTGATGAG
    CAATTCAGAG TGGCAGAAGT AATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237788
    Clone ID Related Accession (Same CDS sequence) XM_024996672.1
    Accession Version XM_024996672.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
    ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT
    CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC
    GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA
    ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT
    TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC
    ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT
    GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC
    ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA
    GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT
    AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG
    CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC
    AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA
    AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA
    GTTCTTCTCT TGCAGTTTGT TACTGGCAGC CGTGAAGAGA TACCTCACGT CCAAGTTCCA
    GGGTCCCACA TGGTGGATTT GCTAATATCA TGGGTGGAAG TGGATTGCAA AACTTCACAA
    TTGCTGCTGT GCCATATACT CCAAATCTTC TACCTACTTC AAGCACATGC ATCAACATGC
    TCAAGTTACC TGAATACCCA AGCAAAGAAA TACTCAAGGA CAGGCTTCTT GTGGCACTAC
    ATTGTGGCAG CTATGGTTAC ACAATGGCAT AATGAAGTCT GGAAAATTCT TCTGACTGCT
    GATGAGCAAT TCAGAGTGGC AGAAGTAATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852440.1
    CDS671..2983
    Translation

    Target ORF information:

    RefSeq Version XM_024996672.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996672.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
    ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT
    CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC
    GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA
    ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT
    TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC
    ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT
    GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC
    ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA
    GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT
    AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG
    CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC
    AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA
    AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA
    GTTCTTCTCT TGCAGTTTGT TACTGGCAGC CGTGAAGAGA TACCTCACGT CCAAGTTCCA
    GGGTCCCACA TGGTGGATTT GCTAATATCA TGGGTGGAAG TGGATTGCAA AACTTCACAA
    TTGCTGCTGT GCCATATACT CCAAATCTTC TACCTACTTC AAGCACATGC ATCAACATGC
    TCAAGTTACC TGAATACCCA AGCAAAGAAA TACTCAAGGA CAGGCTTCTT GTGGCACTAC
    ATTGTGGCAG CTATGGTTAC ACAATGGCAT AATGAAGTCT GGAAAATTCT TCTGACTGCT
    GATGAGCAAT TCAGAGTGGC AGAAGTAATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237789
    Clone ID Related Accession (Same CDS sequence) XM_024996673.1
    Accession Version XM_024996673.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HACE1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
    ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT
    CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC
    GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA
    ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT
    TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC
    ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT
    GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC
    ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA
    GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT
    AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG
    CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC
    AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA
    AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA
    GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA
    GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852441.1
    CDS671..2896
    Translation

    Target ORF information:

    RefSeq Version XM_024996673.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996673.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TACAACACGT CAGTGATGTC GATGTTGAGG 
    ATGCGATGGG GCAGACAGCG CTGCATGTGG CCTGCCAGAA TGGTCACAAG ACGTCATGGT
    CAGAGAGACA CAGCACAGAT TCTTCTATTA CGAGGTGCCA AGTATCTGTC AGATAAAAAC
    GGAGTAACCC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACCTG TGAAGTATTA
    ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAT
    TTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGTCATAA ATTGCTTAGC
    ATATCTAGTA ATTACGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGGAGCCA GGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT
    GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC
    ACTTCTATTT TGCTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAGGGGA ATCTAAACCA
    GATGTGCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCAGGAATGA CTTCGCCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ACAGGAATCC CAAGATTATA TTTGACCACT TTCACTTCCT TCTCGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAGGCAC AGCCATTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGACATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAT AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTCCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAACCCAAT
    AGCAACTCCT CTGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTC TGAACCATAG GCAACTGGTG AACATTTACT TCACGAGATC ATTCTATAAG
    CACATTCTTG GTATTCCTGT GAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC
    AAAAACTTGC AATGGATTTT AGATAATGAT ATTAGTGATT TGGGTCTAGA ATTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CCTTGAAACC TGGGGGTGGA
    AGTATTCTTG TAACACAAAA TAACAAAGCG GAGTACGTCC AGCTTGTCAC TGAACTGCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCATCCC TCATACAACT TTTTGATGAA TACGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ATATTACTCC AGAGGAGAGA
    GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTC ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTCTACCTA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGCAAA
    GAAATACTCA AGGACAGGCT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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