MAST4 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following MAST4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAST4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa228110 XM_024981444.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228111 XM_024981445.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228112 XM_024981446.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228113 XM_024981447.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228114 XM_024981448.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228115 XM_024981449.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228116 XM_024981450.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228117 XM_024981451.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228118 XM_024981452.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228119 XM_024981454.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa94100 XM_015459062.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa95085 XM_015468336.1
Latest version!
Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa228110
    Clone ID Related Accession (Same CDS sequence) XM_024981444.1
    Accession Version XM_024981444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC ACCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GAAGGAGCTG AGTCTTCCCA GAAGAGGAAG TCTGATAGAT
    TCCCAGAAGT GGAATTGCTT GGTCAAACGT TGCCGAACCA GCAACCGGAA AAGCTTGATA
    GGCAATGGAC CATCCCCAGC ATTGCCTCGG CCCCACTCAC CTCTTTCTGC TCATGCAGGA
    AATAGCCCTC AAGATAGTCC AAGAAATTTC TCCCCCAGTG CCTCTGCTCA TTTTTCATTT
    GCACGGAGGA CTGATGGACG CCGCTGGTCC TTGGCTTCTC TCCCCTCCTC TGGCTATGGG
    ACAAACACTC CCAGCTCTAC GGTCTCTTCA TCCTGTTCCT CCCAGGAGAA GTTGCATCAG
    TTACCGTATC AACCGACACC AGATGAATTA CACTTCTTAT CAAAACATTT TTGTACCACT
    GAAAGCATCA CCACAGAGAA CAGATGCAGA AACACACCCA TGCGCCCCCG TTCCCGAAGT
    TTGAGCCCTG GACGTTCTCC CGCCTGCTGT GACCATGAAA TAATTATGAT GAACCATGTC
    TACAAAGAAA GGTTCCCAAA GGCTACAGCT CAGATGGAAG AGCGCCTAAA GGAGATCATC
    ACCAGCTACT CTCCAGACCA CGTTCTCCCC CTTGCAGATG GAGTGCTCAG CTTCACTCAC
    CATCAGATTA TCGAACTGGC TCGCGATTGT TTGGATAAAT CCCACCAGGG TCTCATCACC
    TCACGATACT TCCTTGAATT ACAGCACAAA TTAGATAAGT TACTACAGGA GGCTCACAAT
    CGTTCAGAAA GTGGAGAGCT GGCATTTATT AAACAACTAG TTCGAAAGAT CCTGATTGTT
    ATTGCCCGCC CTGCTCGCTT ATTAGAGTGC CTGGAATTTG ATCCTGAAGA ATTTTACTAC
    CTATTGGAAG CAGCAGAAGG CCATGCCAAA GAAGGACAGG GTATTAAAAC TGACATTCCC
    AGGTACATCA TTAGCCAGCT TGGCCTCAAT AAAGATCCTC TGGAAGAAAT GGCTCAACTG
    GGAAACTATG ATAGTGGGAC AGCAGAAACA CCAGAAACAG ATGAATCAGT GAGTAGCTCT
    AATGCTTCCC TGAAACTTCG AAGGAAGCCT CGGGAAAGTG ATTTTGAAAC GATTAAATTG
    ATCAGCAATG GAGCCTACGG GGCAGTCTAT TTCGTTCGGC ATAAGGAATC CCGGCAGAGG
    TTTGCCATGA AGAAGATTAA CAAACAGAAC CTCATCCTCC GAAACCAGAT CCAGCAGGCC
    TTTGTGGAAC GGGATATCCT GACTTTTGCA GAAAACCCTT TTGTCGTCAG CATGTACTGC
    TCCTTTGAGA CCAGGCGCCA CTTGTGCATG GTCATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACGTTAA TGAAGAACAT GGGTCCTCTC CCCGTAGATA TGGCCAGGAT GTACTTTGCT
    GAGACGGTCC TGGCCTTGGA ATATCTACAT AATTATGGAA TTGTACATAG GGATTTGAAA
    CCAGACAATT TGCTGGTCAC CTCTATGGGG CACATAAAGC TGACCGATTT TGGATTATCC
    AAGGTGGGAC TCATGAGCAT GACTACCAAT CTTTATGAGG GTCATATTGA GAAGGATGCC
    CGAGAGTTCC TGGACAAGCA GGTCTGTGGC ACACCTGAAT ACATTGCACC AGAAGTGATT
    CTGAGGCAGG GCTACGGGAA GCCAGTGGAC TGGTGGGCCA TGGGGATTAT CCTCTATGAA
    TTTCTGGTTG GATGCGTGCC ATTCTTTGGG GACACTCCAG AAGAGCTATT TGGACAAGTC
    ATCAGTGATG AGATCAACTG GCCTGAAAAG GATGAGGCTC CACCCCCTGA TGCCCAGGAT
    CTGATTACCT TGCTCCTCAG GCAGAATCCC CTGGAGAGAC TGGGAACAGG TGGTGCATAT
    GAAGTCAAAC AGCATCGATT CTTCCGTTGC TTAGACTGGA ACAGTTTGCT GAGACAGAAG
    GCAGAATTCA TTCCCCAACT GGAATCTGAA GACGACACAA GTTATTTTGA TACTCGGTCA
    GAGAAGTACC ATCATATGGA GACTGAGGAA GAAGATGACA CGAACGATGA AGACTTTAAT
    GTGGAAATAA GGCAGTTTTC CTCATGTTCA CATAGGTTTT CTAAGGTCTT CAGCAGTATG
    GATCGAGGTA CTCAGAATTC AGGAGAAGAG AAGGAAGACC CTGGGGACAA AACTAAAAGC
    ACAGCTTTGC CGTCCACGGA AACACTAAGC TGGAGTTCAG AGTATTCTGA AATGCAACAG
    TTATCCACAT CCAACTCTTC AGACACTGAA AGCAACAGAC ATAGACGCGG TGCTGGGCTG
    CTTCCCAAGC TGGCTGTTTC AGCAGAGGCA GAACACGACG GGGCCGCTCC CCGCCCCAGA
    GAGCTGCACG AGGAGCCGGA AAAGCCAGCC CTGCCTGCTG CAGAGAATGC TCAGGAGGAG
    CCCGAGGTCA CTACCCCGGC CAGCACCATC AGCAGCTCCA CTCTGTCAGT TGGCAGTTTT
    TCAGAGCACT TGGATCAGAT AAATGGACGG AGCGAGTGTG TGGACAGTAC AGACAATGCC
    TCCAAGCCCT CCAATGAGCC CGCCTCTCAC ATGGCTCGGC AGCGCTTAGA AAGCACAGAG
    AAACAGAAAG CCTCGGGGAA AGTCACAAAG TCCCTTTCTG CCAGTGCCCT GTCCCTCATG
    ATCCCAGGAG ATATGTTTGC TGTCTCTCCG CTGGGAAGTC CGATGTCTCC CCATTCCCTG
    TCCTCGGACC CTTCTTCCTC AAGGGATTCC TCTCCCAGCC GAGATTCTTC AGGAGCTTCT
    GCCAGTCCAC ACCAGCCCAT TGTGATCCAC AGTTTGGGGA AGAACTATGG TTTCACCATT
    CGAGCCATCC GGGTGTACGT GGGAGACAGC GACATTTACA CAGTGCATCA TATCGTTTGG
    AATGTAGAAG AAGGAAGTCC AGCTTGCCAG GCAGGACTGA AAGCTGGGGA TCTGATCACA
    CACATCAATG GAGAACCGGT GCACGGACTT GTCCACACAG AAGTTATAGA GCTCCTGCTA
    AAGAGTGGAA ATAAGGTGTC AATCACGACC ACCCCATTTG AAAACACATC AATCAAAACG
    GGACCGGCCA GGAGAAACAG CTATAAGAGC CGGATGGTGA GGCGGAGCAA GAAATCTAAG
    AAGAAGGAAA GTCTGGAAAG GAGGAGATCC CTTTTCAAAA AACTAGCTAA GCAGCCTTCT
    CCTTTACTCC ACACCAGCCG AAGTTTCTCC TGCTTGAACC GATCCCTGTC GTCAGGGGAG
    AGCCTCCCAG GGTCCCCCAC TCACAGCTTG TCTCCCCGAT CCCCTACACC CAGCTACCGT
    TCCACCCCCG ACTTCCCATC CGGCACTAAC TCCTCCCAGA GCAGCTCCCC AAGCTCCAGC
    GCTCCCAACT CCCCAGCAGG GTCAGGACAC GTCCGGCCCA GCACGCTCCA TGGTCTGGCG
    CCCAAACTCA GCGGGCAGCG CTACCGGTCA GGAAGGCGGA AGTCAGCTGG CAGCATCCCC
    CTGTCTCCGC TGGCCCGGAC ACCCTCTCCG ACCCCACAGC CCACCTCCCC GCAGCGGTCC
    CCATCCCCTC TGCTGGGGCA CTCACTTGGC AATTCCAAGA TGGCTCAAGC CTTTCCCAGC
    AAAATGCACT CCCCACCCAC CATCGTCAGG CACGTCGTGA GGCCCAAGAG TGCAGAACCC
    CCTCGGTCCC CGCTGCTCAA GCGCGTGCAG TCGGAGGAGA AGCTGTCTCC CTCCTATGGA
    GGCGACAAGA AGCACCTGTG CTCCCGCAAG CACAGCCTTG AGGTCACCCA GGAGGAGGTG
    CCGCGGGAGC AGCAACAGCG GGAGGTGACC TTGCAGAGCC TGGAGGAGAA CGTGTGCGAC
    GCGCCTGCCC TCAGCCGGGC CAGGCCCGTG GAGCAGGGCT GCCTGAAACG CCCCGTGTCC
    CGGAAGCTGG GCCGCCAGGA GTCTGTGGAC GAGCTGGACC GAGAGAAGCT AAAGGCCAAG
    GTGGTGGTGA AGAAACCTGA CGGGCTCTCG GAAAAACAGG AATCCCGCCC GAAGCCCCAC
    GGACTCGGCA GTGACTTGGA AAACCTCTGT GCTTTTAGGC TCGAAGAGAG AGACAAGAAA
    ATCTATCCAA AAGCTTCAGA GAGGTCAAAT CATTTTGAAA GCAAAGCAGC CGTGCAGGAG
    GCCCAGCCCC TGTGCAGCCT TTTGAAAGAT GCTCTCCACA AGCAGGCCAA TGTGCGGGCC
    AGCGAGGGTG TCGCCTCTGA GGGGGCAACG GCAGCTGGGG ACCACGGCCA GGGCACCTGT
    GACTTCAAAC GAGCCTCAAC GCACAGCACC CTCCAAGACG GCCTCTGTCA CACCACCCAC
    AGGTCCACCT CCGGGAAGGG GGACACCGCA GAGAAGGCCC CCCAGGCCAA GGAGGGTCTG
    CGGTGTGAGA AGTTAGACGG CAAGTTGGCC AACATCGATT ACCTCCGAAA GAAGATGTCC
    CTTGAAGACA AAGACGAGAG CCTCTGTTCT GTGCTCAAGC CCAAGGTGAC AGCTGGTGCC
    CAGGACTGCC TGCCTGGGAG CCTGGGCCGG CCCGTGGGAG GGCAGCAGGA GGCCCCGCCA
    GCCTCCGAGG CCCGCACACC GTTCATCGGC AGCGCCCACG CGGCTCAGCT CAGCGCCGTC
    TCCTTTGTCC CTCTCAAGGC CCTTTCTGGC CGGGTGGACA GTGGAGCGGA GAAGCCGGGC
    TTGGCTGCCC CAGAGTCGCC TGTCCGGAAA AGCCCCTCCG AGTATAAGCT GGAAGGGCGG
    TCTGTGTCGT GCCTGAAGCC CATTGAAGGC ACCTTGGACA TCGCTCTCCT GTCGGGACCT
    CAGGCCTCCA AGACAGAGCT GCCTTCCCCG GAGCCTGCCC AGAGCCCCAG CCCAGGCAGT
    GATGTGGAGT CCCCCATGCC CCTCGCTCTC CCCAGCAGCA CAGGGAGGAA GGCTGACACT
    GCGGGTCAGA GAGAGTCCTC CCCTGCCGGC TTCAAGGTGA ACAAATCCTA CCTGCTTGAG
    CCCCGGTTCC CACCGGCCAG CCGGGGTCTG CAGAGTTCAC CGGTGGTTGC CCCGCCTGAC
    CCAGAGCTGA AGCTGGACAA GAAGGTCTCC CAGGCAGCCC GTGCCCCAGC GACCATCATG
    GAGAGCCATC CCCAGCGGGG AGAGGGCCAC CCCAGCCAAC ACCAAGACCA CAGCCCCGAC
    GTTAAGCTCC TTCCCTTCCC AGGGCAGAAC CTCCAAGGTG CCAACACGTC TAGATCCCGC
    AGCCCACTCT TGCCTGAAAG TGTCCCCCCT AGAGAGAAGC CGAGTGGGAG AGAGTTGTCC
    GAAAGGGGCC CTTCCATAGT CGGGAGCGAG TGGTCAGCTG TGAGGCCCGA CATCCGCAGA
    GACCCCTCCA AGGAGCTGTG TCCCCCAGAA GCTGCTAAAC CCAGCGACAA CTCCCAAACC
    CCCCCTGTGG TGGGGAGGAC CCGCCTGGAT GTCTCCACCC AGACCCAGGC CTTGGAGAAA
    GCATGCGGGC CTTGCGGGAG AGCAAAGCCC AGAGACGGCC GAGAGGAGGT GAGGCTGCCG
    GCCAGAGAGG ACTCCTCTTT GCGCTCTGCG GGGGCTCCTT GTGAGAGAGA GCTGGGCGCA
    GAGAGAGGCC TGGAATCCAA GATGGAACCT GCTTCTACCA GGTGGTCTCT GGAGCCGCCC
    AGGCCAGAGA GTGAGAAGAG TGGAAGGCCT TCTGGTTTCC AGTCTGTGCA GAAAGACGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAACCT CTGCAAAAGT ACCTCACCAG TTCTCAGCCG
    GGTCCCAAGG AACCCGAGAG GAAAGAACAG CCCCTGCAAA AGCACCTCCC CAGTAGTTCT
    CAGCCGGGTC CCAAGGAACC CGAGAGGAAA GAACAGCCCC TGCAAAAGCA CGTCACCAGT
    AGTTCTCAGC CGGGTCCCAA GGAACCGGAG AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA CCGGAGAGGA AAGAACAGCC CCTGCAAAAG
    CACGTCACCA GTAGTTCTCA GCTGGGTCCC AAGGAACCCG AGAGGAAAGA ACAGCCTCTG
    CAAAAGCACC TCCCCAGTAG TTCTCAGCCG GGTCCCAAGG AACTGGAAAG GAAAGAACAG
    TGTTTGCAGA AGCACCTCAC CAGTAGCTCT CAGCTGGGTC CCAAGGAACC AGAAATGAAA
    GAACAGCCCC CACAAAAGCA CGTCACCAGT AGTTCTCAAC TGTGTCCCAA GGAACTGGAA
    AGGAAAGAAC AACCTCCACA AAAGCACCTC ACTATTAGCT CTCAGCTGAG TCCCAAGGAA
    CCAGAAAGGA AAGAACAGCC TCTGCAAGAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAACCGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACT TCACCAGTAG TTCTCAGCTG
    GGTCCCAAGG AACCAGAAAC GAAAGAACAG CCTCTGCAAA AGCACCTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC GGAAAGGAAA GAACAGCTTC TGCAAGAGCA CCTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAACCGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACCTC
    ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA CCAGAAATGA AAGAACAGCC TCTGCAAAAG
    CACCTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCAG AAAGGAAAGA ACAGCCTCTG
    CAAAAGCACG TCACCAGTAG CTCTCAGCTG AGCCCCAAGG AGCCGGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CAGTAGCTGT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CGTCACCAGT AGCTCTCAGC TGAGCCCCAA GGAGCCAGAA
    AGGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAA
    CCAGAAAGGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCAG AAAGGAAAGA ACAGCTTCTG CAAAAGCACC TCACCATTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CTTCTGCAAA AGCACCTCAC CATTAGCTCT
    CAGCTGAGTC CCAAGGAGCC GGAAAAGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCATTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAGCCGG AAAGGAAAGA ACAGCTTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AGCCGGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CGTCACCAGT AGCTCTCAGC TGAGTCCCAA GGAACCGGAA
    AGGAAAGAAC AGTATTTGCA GAAGCACCTC ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA
    CCAGAAACGA AAGAACAGCC TCTGCAAAAG CACCTCACCA GTAGTTCTCA GGTGGGTCCC
    AAGGATCCGG AAAGGAAAGA ACAGCCCCCA CAAAAGCACC TCACCAGTAA GTCTCAGCCG
    GGTCCCAAGG AACCGGAAAG GAAAGAACAG TGTTTGCAAA AGCACCTCAC CAGTAGTTCT
    CAGCCGGGTC CCAAGGAACT GGAAAGGAAA GAACAACCTC CACTAAAGCA TGTCACCAGT
    AGCTCTCAGC TGGGTCCCAG GGAACCAGAA AGGAAAGAAC AGCCCCCACA AAAGCACCTC
    ACCAGTAGCT GTCAGCCCCC ACCCCCTACC AAAGAGCTCC CTGGCCTGGC TTGTCAGCAC
    TGCATTCTGC CCAGCCACCC TGCTGCCTCT CTGCTGGGGA GCAAGCCCTG TGCTATAGAC
    TCCAGCTTCC AGGATGCTCT CAGGCCCACT GCTGTGCGCA GTGACAGCAG CAGCCACAAG
    CCCAGGTCTG ACCCCGACCT GGGCCCTCCA AAGTCTAAGT TCCCAGACCG GCCCCTCTCC
    TCCCAGAAGC CGAGCGCTGA GGCTGCAGTG AGCAAAGAGC CTGGCGCTCC GACCCTCGGC
    AGAGCTGGGG TGGGGAGTGG TAAGGGTGTG TCAGAGGAGT TCCCTGTCCT TCCAGATCCC
    CAGAACACAG CCCGTGAAGT GATTGGCCAG GGAGCCAGTG GGTACTCAGG CCCCCTGCCC
    ATGGAAGGGG GTCCTCTAGA CACAAAGCTG AGACCCACCA GCGCTCGGTG TCCCCCAGAG
    GCACTGGAGA TGCTTGGACA TCCACCAGGG CGAGGGCTCC TAGGGGCCAG TGAGTCAGTG
    GACCAGAAAC TCCCCACCTT CAGGGAAAGG CAAAACCTGT CTCCAAAGGC CCCCACACCA
    TCCACTGTGA AAGATTGTCC CCTTCCGTGC AAACAGACAG ACAGGAGCCC GAGTCCTCTG
    GCCACCAGCA CCAACGCTGG CAAGTCTGAA GGCAAGAAAT GCACTGAAGC ACTTTACACC
    CCTGCGGATG GTGAGAAGCT GGGGGCCGGC TTGTCCTCGG TGCACAGCGA GGCTGGGCCT
    AAGGGCACAG AGAAGCCGGC AGCAGCCGCT GGGAAGGGCT GGCCAGAGGC CAAGGGGAAA
    GGACTCAGTC CCCAGAAGCC ACTGACCGAG GCCAGCAAGC CCAGCGGCAT GAAGCGGTCA
    CCTTCAGCCA CGGTGCAGAG CTCTCTCCGC TGTGCCACCC TCCCAGAGAA GTCTCTGAGC
    TACTCCTGTG GCTTTCCCGA GGCCAGACCA GCAGTTCCAG AGACTTCTAC CACCTCCAGC
    GACACCTCCT CTGCCAAGGC TGCTGGGCCT GCAGCAGAAT CCCCAGCCTC CAGCAGCAGG
    GACCAGCAGA AACCCCTGCC TGGTGGGGAT GGCAGAACCC AAATGACCAA GAGCGACTCC
    TTGCCATGCT TCCGCAGCTC AGCCATCACT CTGGAGTCCC AGCACCCCAG CCCTGGCCTG
    GTGGTGGTCA CCAGCCACCG GGACAGGGCC CTCTCAGTAA CCGCCACCGT GGGAGAAACC
    AAAGGGAAAG AGCCAGCCCC CACCCAGCCA GCTCAGACCA GGAACCAGAA CGTGAGCAGA
    GAGGCGGCCA AGCCGTCCCC CGCTCCAAGC ACTGAGCGCC CCATCGTTCT TCCTTCTGAG
    AAGGACACTG GGCTGCGACA GAGGCGGGGG AAAGAGAGTT TGCGCGGCAG CCCCCACAAA
    AAGGCCTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837212.1
    CDS263..10294
    Translation

    Target ORF information:

    RefSeq Version XM_024981444.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC ACCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GAAGGAGCTG AGTCTTCCCA GAAGAGGAAG TCTGATAGAT
    TCCCAGAAGT GGAATTGCTT GGTCAAACGT TGCCGAACCA GCAACCGGAA AAGCTTGATA
    GGCAATGGAC CATCCCCAGC ATTGCCTCGG CCCCACTCAC CTCTTTCTGC TCATGCAGGA
    AATAGCCCTC AAGATAGTCC AAGAAATTTC TCCCCCAGTG CCTCTGCTCA TTTTTCATTT
    GCACGGAGGA CTGATGGACG CCGCTGGTCC TTGGCTTCTC TCCCCTCCTC TGGCTATGGG
    ACAAACACTC CCAGCTCTAC GGTCTCTTCA TCCTGTTCCT CCCAGGAGAA GTTGCATCAG
    TTACCGTATC AACCGACACC AGATGAATTA CACTTCTTAT CAAAACATTT TTGTACCACT
    GAAAGCATCA CCACAGAGAA CAGATGCAGA AACACACCCA TGCGCCCCCG TTCCCGAAGT
    TTGAGCCCTG GACGTTCTCC CGCCTGCTGT GACCATGAAA TAATTATGAT GAACCATGTC
    TACAAAGAAA GGTTCCCAAA GGCTACAGCT CAGATGGAAG AGCGCCTAAA GGAGATCATC
    ACCAGCTACT CTCCAGACCA CGTTCTCCCC CTTGCAGATG GAGTGCTCAG CTTCACTCAC
    CATCAGATTA TCGAACTGGC TCGCGATTGT TTGGATAAAT CCCACCAGGG TCTCATCACC
    TCACGATACT TCCTTGAATT ACAGCACAAA TTAGATAAGT TACTACAGGA GGCTCACAAT
    CGTTCAGAAA GTGGAGAGCT GGCATTTATT AAACAACTAG TTCGAAAGAT CCTGATTGTT
    ATTGCCCGCC CTGCTCGCTT ATTAGAGTGC CTGGAATTTG ATCCTGAAGA ATTTTACTAC
    CTATTGGAAG CAGCAGAAGG CCATGCCAAA GAAGGACAGG GTATTAAAAC TGACATTCCC
    AGGTACATCA TTAGCCAGCT TGGCCTCAAT AAAGATCCTC TGGAAGAAAT GGCTCAACTG
    GGAAACTATG ATAGTGGGAC AGCAGAAACA CCAGAAACAG ATGAATCAGT GAGTAGCTCT
    AATGCTTCCC TGAAACTTCG AAGGAAGCCT CGGGAAAGTG ATTTTGAAAC GATTAAATTG
    ATCAGCAATG GAGCCTACGG GGCAGTCTAT TTCGTTCGGC ATAAGGAATC CCGGCAGAGG
    TTTGCCATGA AGAAGATTAA CAAACAGAAC CTCATCCTCC GAAACCAGAT CCAGCAGGCC
    TTTGTGGAAC GGGATATCCT GACTTTTGCA GAAAACCCTT TTGTCGTCAG CATGTACTGC
    TCCTTTGAGA CCAGGCGCCA CTTGTGCATG GTCATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACGTTAA TGAAGAACAT GGGTCCTCTC CCCGTAGATA TGGCCAGGAT GTACTTTGCT
    GAGACGGTCC TGGCCTTGGA ATATCTACAT AATTATGGAA TTGTACATAG GGATTTGAAA
    CCAGACAATT TGCTGGTCAC CTCTATGGGG CACATAAAGC TGACCGATTT TGGATTATCC
    AAGGTGGGAC TCATGAGCAT GACTACCAAT CTTTATGAGG GTCATATTGA GAAGGATGCC
    CGAGAGTTCC TGGACAAGCA GGTCTGTGGC ACACCTGAAT ACATTGCACC AGAAGTGATT
    CTGAGGCAGG GCTACGGGAA GCCAGTGGAC TGGTGGGCCA TGGGGATTAT CCTCTATGAA
    TTTCTGGTTG GATGCGTGCC ATTCTTTGGG GACACTCCAG AAGAGCTATT TGGACAAGTC
    ATCAGTGATG AGATCAACTG GCCTGAAAAG GATGAGGCTC CACCCCCTGA TGCCCAGGAT
    CTGATTACCT TGCTCCTCAG GCAGAATCCC CTGGAGAGAC TGGGAACAGG TGGTGCATAT
    GAAGTCAAAC AGCATCGATT CTTCCGTTGC TTAGACTGGA ACAGTTTGCT GAGACAGAAG
    GCAGAATTCA TTCCCCAACT GGAATCTGAA GACGACACAA GTTATTTTGA TACTCGGTCA
    GAGAAGTACC ATCATATGGA GACTGAGGAA GAAGATGACA CGAACGATGA AGACTTTAAT
    GTGGAAATAA GGCAGTTTTC CTCATGTTCA CATAGGTTTT CTAAGGTCTT CAGCAGTATG
    GATCGAGGTA CTCAGAATTC AGGAGAAGAG AAGGAAGACC CTGGGGACAA AACTAAAAGC
    ACAGCTTTGC CGTCCACGGA AACACTAAGC TGGAGTTCAG AGTATTCTGA AATGCAACAG
    TTATCCACAT CCAACTCTTC AGACACTGAA AGCAACAGAC ATAGACGCGG TGCTGGGCTG
    CTTCCCAAGC TGGCTGTTTC AGCAGAGGCA GAACACGACG GGGCCGCTCC CCGCCCCAGA
    GAGCTGCACG AGGAGCCGGA AAAGCCAGCC CTGCCTGCTG CAGAGAATGC TCAGGAGGAG
    CCCGAGGTCA CTACCCCGGC CAGCACCATC AGCAGCTCCA CTCTGTCAGT TGGCAGTTTT
    TCAGAGCACT TGGATCAGAT AAATGGACGG AGCGAGTGTG TGGACAGTAC AGACAATGCC
    TCCAAGCCCT CCAATGAGCC CGCCTCTCAC ATGGCTCGGC AGCGCTTAGA AAGCACAGAG
    AAACAGAAAG CCTCGGGGAA AGTCACAAAG TCCCTTTCTG CCAGTGCCCT GTCCCTCATG
    ATCCCAGGAG ATATGTTTGC TGTCTCTCCG CTGGGAAGTC CGATGTCTCC CCATTCCCTG
    TCCTCGGACC CTTCTTCCTC AAGGGATTCC TCTCCCAGCC GAGATTCTTC AGGAGCTTCT
    GCCAGTCCAC ACCAGCCCAT TGTGATCCAC AGTTTGGGGA AGAACTATGG TTTCACCATT
    CGAGCCATCC GGGTGTACGT GGGAGACAGC GACATTTACA CAGTGCATCA TATCGTTTGG
    AATGTAGAAG AAGGAAGTCC AGCTTGCCAG GCAGGACTGA AAGCTGGGGA TCTGATCACA
    CACATCAATG GAGAACCGGT GCACGGACTT GTCCACACAG AAGTTATAGA GCTCCTGCTA
    AAGAGTGGAA ATAAGGTGTC AATCACGACC ACCCCATTTG AAAACACATC AATCAAAACG
    GGACCGGCCA GGAGAAACAG CTATAAGAGC CGGATGGTGA GGCGGAGCAA GAAATCTAAG
    AAGAAGGAAA GTCTGGAAAG GAGGAGATCC CTTTTCAAAA AACTAGCTAA GCAGCCTTCT
    CCTTTACTCC ACACCAGCCG AAGTTTCTCC TGCTTGAACC GATCCCTGTC GTCAGGGGAG
    AGCCTCCCAG GGTCCCCCAC TCACAGCTTG TCTCCCCGAT CCCCTACACC CAGCTACCGT
    TCCACCCCCG ACTTCCCATC CGGCACTAAC TCCTCCCAGA GCAGCTCCCC AAGCTCCAGC
    GCTCCCAACT CCCCAGCAGG GTCAGGACAC GTCCGGCCCA GCACGCTCCA TGGTCTGGCG
    CCCAAACTCA GCGGGCAGCG CTACCGGTCA GGAAGGCGGA AGTCAGCTGG CAGCATCCCC
    CTGTCTCCGC TGGCCCGGAC ACCCTCTCCG ACCCCACAGC CCACCTCCCC GCAGCGGTCC
    CCATCCCCTC TGCTGGGGCA CTCACTTGGC AATTCCAAGA TGGCTCAAGC CTTTCCCAGC
    AAAATGCACT CCCCACCCAC CATCGTCAGG CACGTCGTGA GGCCCAAGAG TGCAGAACCC
    CCTCGGTCCC CGCTGCTCAA GCGCGTGCAG TCGGAGGAGA AGCTGTCTCC CTCCTATGGA
    GGCGACAAGA AGCACCTGTG CTCCCGCAAG CACAGCCTTG AGGTCACCCA GGAGGAGGTG
    CCGCGGGAGC AGCAACAGCG GGAGGTGACC TTGCAGAGCC TGGAGGAGAA CGTGTGCGAC
    GCGCCTGCCC TCAGCCGGGC CAGGCCCGTG GAGCAGGGCT GCCTGAAACG CCCCGTGTCC
    CGGAAGCTGG GCCGCCAGGA GTCTGTGGAC GAGCTGGACC GAGAGAAGCT AAAGGCCAAG
    GTGGTGGTGA AGAAACCTGA CGGGCTCTCG GAAAAACAGG AATCCCGCCC GAAGCCCCAC
    GGACTCGGCA GTGACTTGGA AAACCTCTGT GCTTTTAGGC TCGAAGAGAG AGACAAGAAA
    ATCTATCCAA AAGCTTCAGA GAGGTCAAAT CATTTTGAAA GCAAAGCAGC CGTGCAGGAG
    GCCCAGCCCC TGTGCAGCCT TTTGAAAGAT GCTCTCCACA AGCAGGCCAA TGTGCGGGCC
    AGCGAGGGTG TCGCCTCTGA GGGGGCAACG GCAGCTGGGG ACCACGGCCA GGGCACCTGT
    GACTTCAAAC GAGCCTCAAC GCACAGCACC CTCCAAGACG GCCTCTGTCA CACCACCCAC
    AGGTCCACCT CCGGGAAGGG GGACACCGCA GAGAAGGCCC CCCAGGCCAA GGAGGGTCTG
    CGGTGTGAGA AGTTAGACGG CAAGTTGGCC AACATCGATT ACCTCCGAAA GAAGATGTCC
    CTTGAAGACA AAGACGAGAG CCTCTGTTCT GTGCTCAAGC CCAAGGTGAC AGCTGGTGCC
    CAGGACTGCC TGCCTGGGAG CCTGGGCCGG CCCGTGGGAG GGCAGCAGGA GGCCCCGCCA
    GCCTCCGAGG CCCGCACACC GTTCATCGGC AGCGCCCACG CGGCTCAGCT CAGCGCCGTC
    TCCTTTGTCC CTCTCAAGGC CCTTTCTGGC CGGGTGGACA GTGGAGCGGA GAAGCCGGGC
    TTGGCTGCCC CAGAGTCGCC TGTCCGGAAA AGCCCCTCCG AGTATAAGCT GGAAGGGCGG
    TCTGTGTCGT GCCTGAAGCC CATTGAAGGC ACCTTGGACA TCGCTCTCCT GTCGGGACCT
    CAGGCCTCCA AGACAGAGCT GCCTTCCCCG GAGCCTGCCC AGAGCCCCAG CCCAGGCAGT
    GATGTGGAGT CCCCCATGCC CCTCGCTCTC CCCAGCAGCA CAGGGAGGAA GGCTGACACT
    GCGGGTCAGA GAGAGTCCTC CCCTGCCGGC TTCAAGGTGA ACAAATCCTA CCTGCTTGAG
    CCCCGGTTCC CACCGGCCAG CCGGGGTCTG CAGAGTTCAC CGGTGGTTGC CCCGCCTGAC
    CCAGAGCTGA AGCTGGACAA GAAGGTCTCC CAGGCAGCCC GTGCCCCAGC GACCATCATG
    GAGAGCCATC CCCAGCGGGG AGAGGGCCAC CCCAGCCAAC ACCAAGACCA CAGCCCCGAC
    GTTAAGCTCC TTCCCTTCCC AGGGCAGAAC CTCCAAGGTG CCAACACGTC TAGATCCCGC
    AGCCCACTCT TGCCTGAAAG TGTCCCCCCT AGAGAGAAGC CGAGTGGGAG AGAGTTGTCC
    GAAAGGGGCC CTTCCATAGT CGGGAGCGAG TGGTCAGCTG TGAGGCCCGA CATCCGCAGA
    GACCCCTCCA AGGAGCTGTG TCCCCCAGAA GCTGCTAAAC CCAGCGACAA CTCCCAAACC
    CCCCCTGTGG TGGGGAGGAC CCGCCTGGAT GTCTCCACCC AGACCCAGGC CTTGGAGAAA
    GCATGCGGGC CTTGCGGGAG AGCAAAGCCC AGAGACGGCC GAGAGGAGGT GAGGCTGCCG
    GCCAGAGAGG ACTCCTCTTT GCGCTCTGCG GGGGCTCCTT GTGAGAGAGA GCTGGGCGCA
    GAGAGAGGCC TGGAATCCAA GATGGAACCT GCTTCTACCA GGTGGTCTCT GGAGCCGCCC
    AGGCCAGAGA GTGAGAAGAG TGGAAGGCCT TCTGGTTTCC AGTCTGTGCA GAAAGACGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAACCT CTGCAAAAGT ACCTCACCAG TTCTCAGCCG
    GGTCCCAAGG AACCCGAGAG GAAAGAACAG CCCCTGCAAA AGCACCTCCC CAGTAGTTCT
    CAGCCGGGTC CCAAGGAACC CGAGAGGAAA GAACAGCCCC TGCAAAAGCA CGTCACCAGT
    AGTTCTCAGC CGGGTCCCAA GGAACCGGAG AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA CCGGAGAGGA AAGAACAGCC CCTGCAAAAG
    CACGTCACCA GTAGTTCTCA GCTGGGTCCC AAGGAACCCG AGAGGAAAGA ACAGCCTCTG
    CAAAAGCACC TCCCCAGTAG TTCTCAGCCG GGTCCCAAGG AACTGGAAAG GAAAGAACAG
    TGTTTGCAGA AGCACCTCAC CAGTAGCTCT CAGCTGGGTC CCAAGGAACC AGAAATGAAA
    GAACAGCCCC CACAAAAGCA CGTCACCAGT AGTTCTCAAC TGTGTCCCAA GGAACTGGAA
    AGGAAAGAAC AACCTCCACA AAAGCACCTC ACTATTAGCT CTCAGCTGAG TCCCAAGGAA
    CCAGAAAGGA AAGAACAGCC TCTGCAAGAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAACCGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACT TCACCAGTAG TTCTCAGCTG
    GGTCCCAAGG AACCAGAAAC GAAAGAACAG CCTCTGCAAA AGCACCTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC GGAAAGGAAA GAACAGCTTC TGCAAGAGCA CCTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAACCGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACCTC
    ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA CCAGAAATGA AAGAACAGCC TCTGCAAAAG
    CACCTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCAG AAAGGAAAGA ACAGCCTCTG
    CAAAAGCACG TCACCAGTAG CTCTCAGCTG AGCCCCAAGG AGCCGGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CAGTAGCTGT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CGTCACCAGT AGCTCTCAGC TGAGCCCCAA GGAGCCAGAA
    AGGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAA
    CCAGAAAGGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCAG AAAGGAAAGA ACAGCTTCTG CAAAAGCACC TCACCATTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CTTCTGCAAA AGCACCTCAC CATTAGCTCT
    CAGCTGAGTC CCAAGGAGCC GGAAAAGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCATTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAGCCGG AAAGGAAAGA ACAGCTTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AGCCGGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CGTCACCAGT AGCTCTCAGC TGAGTCCCAA GGAACCGGAA
    AGGAAAGAAC AGTATTTGCA GAAGCACCTC ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA
    CCAGAAACGA AAGAACAGCC TCTGCAAAAG CACCTCACCA GTAGTTCTCA GGTGGGTCCC
    AAGGATCCGG AAAGGAAAGA ACAGCCCCCA CAAAAGCACC TCACCAGTAA GTCTCAGCCG
    GGTCCCAAGG AACCGGAAAG GAAAGAACAG TGTTTGCAAA AGCACCTCAC CAGTAGTTCT
    CAGCCGGGTC CCAAGGAACT GGAAAGGAAA GAACAACCTC CACTAAAGCA TGTCACCAGT
    AGCTCTCAGC TGGGTCCCAG GGAACCAGAA AGGAAAGAAC AGCCCCCACA AAAGCACCTC
    ACCAGTAGCT GTCAGCCCCC ACCCCCTACC AAAGAGCTCC CTGGCCTGGC TTGTCAGCAC
    TGCATTCTGC CCAGCCACCC TGCTGCCTCT CTGCTGGGGA GCAAGCCCTG TGCTATAGAC
    TCCAGCTTCC AGGATGCTCT CAGGCCCACT GCTGTGCGCA GTGACAGCAG CAGCCACAAG
    CCCAGGTCTG ACCCCGACCT GGGCCCTCCA AAGTCTAAGT TCCCAGACCG GCCCCTCTCC
    TCCCAGAAGC CGAGCGCTGA GGCTGCAGTG AGCAAAGAGC CTGGCGCTCC GACCCTCGGC
    AGAGCTGGGG TGGGGAGTGG TAAGGGTGTG TCAGAGGAGT TCCCTGTCCT TCCAGATCCC
    CAGAACACAG CCCGTGAAGT GATTGGCCAG GGAGCCAGTG GGTACTCAGG CCCCCTGCCC
    ATGGAAGGGG GTCCTCTAGA CACAAAGCTG AGACCCACCA GCGCTCGGTG TCCCCCAGAG
    GCACTGGAGA TGCTTGGACA TCCACCAGGG CGAGGGCTCC TAGGGGCCAG TGAGTCAGTG
    GACCAGAAAC TCCCCACCTT CAGGGAAAGG CAAAACCTGT CTCCAAAGGC CCCCACACCA
    TCCACTGTGA AAGATTGTCC CCTTCCGTGC AAACAGACAG ACAGGAGCCC GAGTCCTCTG
    GCCACCAGCA CCAACGCTGG CAAGTCTGAA GGCAAGAAAT GCACTGAAGC ACTTTACACC
    CCTGCGGATG GTGAGAAGCT GGGGGCCGGC TTGTCCTCGG TGCACAGCGA GGCTGGGCCT
    AAGGGCACAG AGAAGCCGGC AGCAGCCGCT GGGAAGGGCT GGCCAGAGGC CAAGGGGAAA
    GGACTCAGTC CCCAGAAGCC ACTGACCGAG GCCAGCAAGC CCAGCGGCAT GAAGCGGTCA
    CCTTCAGCCA CGGTGCAGAG CTCTCTCCGC TGTGCCACCC TCCCAGAGAA GTCTCTGAGC
    TACTCCTGTG GCTTTCCCGA GGCCAGACCA GCAGTTCCAG AGACTTCTAC CACCTCCAGC
    GACACCTCCT CTGCCAAGGC TGCTGGGCCT GCAGCAGAAT CCCCAGCCTC CAGCAGCAGG
    GACCAGCAGA AACCCCTGCC TGGTGGGGAT GGCAGAACCC AAATGACCAA GAGCGACTCC
    TTGCCATGCT TCCGCAGCTC AGCCATCACT CTGGAGTCCC AGCACCCCAG CCCTGGCCTG
    GTGGTGGTCA CCAGCCACCG GGACAGGGCC CTCTCAGTAA CCGCCACCGT GGGAGAAACC
    AAAGGGAAAG AGCCAGCCCC CACCCAGCCA GCTCAGACCA GGAACCAGAA CGTGAGCAGA
    GAGGCGGCCA AGCCGTCCCC CGCTCCAAGC ACTGAGCGCC CCATCGTTCT TCCTTCTGAG
    AAGGACACTG GGCTGCGACA GAGGCGGGGG AAAGAGAGTT TGCGCGGCAG CCCCCACAAA
    AAGGCCTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228111
    Clone ID Related Accession (Same CDS sequence) XM_024981445.1
    Accession Version XM_024981445.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC ACCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GAAGGAGCTG AGTCTTCCCA GAAGAGGAAG TCTTTGCCGA
    ACCAGCAACC GGAAAAGCTT GATAGGCAAT GGACCATCCC CAGCATTGCC TCGGCCCCAC
    TCACCTCTTT CTGCTCATGC AGGAAATAGC CCTCAAGATA GTCCAAGAAA TTTCTCCCCC
    AGTGCCTCTG CTCATTTTTC ATTTGCACGG AGGACTGATG GACGCCGCTG GTCCTTGGCT
    TCTCTCCCCT CCTCTGGCTA TGGGACAAAC ACTCCCAGCT CTACGGTCTC TTCATCCTGT
    TCCTCCCAGG AGAAGTTGCA TCAGTTACCG TATCAACCGA CACCAGATGA ATTACACTTC
    TTATCAAAAC ATTTTTGTAC CACTGAAAGC ATCACCACAG AGAACAGATG CAGAAACACA
    CCCATGCGCC CCCGTTCCCG AAGTTTGAGC CCTGGACGTT CTCCCGCCTG CTGTGACCAT
    GAAATAATTA TGATGAACCA TGTCTACAAA GAAAGGTTCC CAAAGGCTAC AGCTCAGATG
    GAAGAGCGCC TAAAGGAGAT CATCACCAGC TACTCTCCAG ACCACGTTCT CCCCCTTGCA
    GATGGAGTGC TCAGCTTCAC TCACCATCAG ATTATCGAAC TGGCTCGCGA TTGTTTGGAT
    AAATCCCACC AGGGTCTCAT CACCTCACGA TACTTCCTTG AATTACAGCA CAAATTAGAT
    AAGTTACTAC AGGAGGCTCA CAATCGTTCA GAAAGTGGAG AGCTGGCATT TATTAAACAA
    CTAGTTCGAA AGATCCTGAT TGTTATTGCC CGCCCTGCTC GCTTATTAGA GTGCCTGGAA
    TTTGATCCTG AAGAATTTTA CTACCTATTG GAAGCAGCAG AAGGCCATGC CAAAGAAGGA
    CAGGGTATTA AAACTGACAT TCCCAGGTAC ATCATTAGCC AGCTTGGCCT CAATAAAGAT
    CCTCTGGAAG AAATGGCTCA ACTGGGAAAC TATGATAGTG GGACAGCAGA AACACCAGAA
    ACAGATGAAT CAGTGAGTAG CTCTAATGCT TCCCTGAAAC TTCGAAGGAA GCCTCGGGAA
    AGTGATTTTG AAACGATTAA ATTGATCAGC AATGGAGCCT ACGGGGCAGT CTATTTCGTT
    CGGCATAAGG AATCCCGGCA GAGGTTTGCC ATGAAGAAGA TTAACAAACA GAACCTCATC
    CTCCGAAACC AGATCCAGCA GGCCTTTGTG GAACGGGATA TCCTGACTTT TGCAGAAAAC
    CCTTTTGTCG TCAGCATGTA CTGCTCCTTT GAGACCAGGC GCCACTTGTG CATGGTCATG
    GAGTATGTGG AAGGGGGTGA CTGTGCCACG TTAATGAAGA ACATGGGTCC TCTCCCCGTA
    GATATGGCCA GGATGTACTT TGCTGAGACG GTCCTGGCCT TGGAATATCT ACATAATTAT
    GGAATTGTAC ATAGGGATTT GAAACCAGAC AATTTGCTGG TCACCTCTAT GGGGCACATA
    AAGCTGACCG ATTTTGGATT ATCCAAGGTG GGACTCATGA GCATGACTAC CAATCTTTAT
    GAGGGTCATA TTGAGAAGGA TGCCCGAGAG TTCCTGGACA AGCAGGTCTG TGGCACACCT
    GAATACATTG CACCAGAAGT GATTCTGAGG CAGGGCTACG GGAAGCCAGT GGACTGGTGG
    GCCATGGGGA TTATCCTCTA TGAATTTCTG GTTGGATGCG TGCCATTCTT TGGGGACACT
    CCAGAAGAGC TATTTGGACA AGTCATCAGT GATGAGATCA ACTGGCCTGA AAAGGATGAG
    GCTCCACCCC CTGATGCCCA GGATCTGATT ACCTTGCTCC TCAGGCAGAA TCCCCTGGAG
    AGACTGGGAA CAGGTGGTGC ATATGAAGTC AAACAGCATC GATTCTTCCG TTGCTTAGAC
    TGGAACAGTT TGCTGAGACA GAAGGCAGAA TTCATTCCCC AACTGGAATC TGAAGACGAC
    ACAAGTTATT TTGATACTCG GTCAGAGAAG TACCATCATA TGGAGACTGA GGAAGAAGAT
    GACACGAACG ATGAAGACTT TAATGTGGAA ATAAGGCAGT TTTCCTCATG TTCACATAGG
    TTTTCTAAGG TCTTCAGCAG TATGGATCGA GGTACTCAGA ATTCAGGAGA AGAGAAGGAA
    GACCCTGGGG ACAAAACTAA AAGCACAGCT TTGCCGTCCA CGGAAACACT AAGCTGGAGT
    TCAGAGTATT CTGAAATGCA ACAGTTATCC ACATCCAACT CTTCAGACAC TGAAAGCAAC
    AGACATAGAC GCGGTGCTGG GCTGCTTCCC AAGCTGGCTG TTTCAGCAGA GGCAGAACAC
    GACGGGGCCG CTCCCCGCCC CAGAGAGCTG CACGAGGAGC CGGAAAAGCC AGCCCTGCCT
    GCTGCAGAGA ATGCTCAGGA GGAGCCCGAG GTCACTACCC CGGCCAGCAC CATCAGCAGC
    TCCACTCTGT CAGTTGGCAG TTTTTCAGAG CACTTGGATC AGATAAATGG ACGGAGCGAG
    TGTGTGGACA GTACAGACAA TGCCTCCAAG CCCTCCAATG AGCCCGCCTC TCACATGGCT
    CGGCAGCGCT TAGAAAGCAC AGAGAAACAG AAAGCCTCGG GGAAAGTCAC AAAGTCCCTT
    TCTGCCAGTG CCCTGTCCCT CATGATCCCA GGAGATATGT TTGCTGTCTC TCCGCTGGGA
    AGTCCGATGT CTCCCCATTC CCTGTCCTCG GACCCTTCTT CCTCAAGGGA TTCCTCTCCC
    AGCCGAGATT CTTCAGGAGC TTCTGCCAGT CCACACCAGC CCATTGTGAT CCACAGTTTG
    GGGAAGAACT ATGGTTTCAC CATTCGAGCC ATCCGGGTGT ACGTGGGAGA CAGCGACATT
    TACACAGTGC ATCATATCGT TTGGAATGTA GAAGAAGGAA GTCCAGCTTG CCAGGCAGGA
    CTGAAAGCTG GGGATCTGAT CACACACATC AATGGAGAAC CGGTGCACGG ACTTGTCCAC
    ACAGAAGTTA TAGAGCTCCT GCTAAAGAGT GGAAATAAGG TGTCAATCAC GACCACCCCA
    TTTGAAAACA CATCAATCAA AACGGGACCG GCCAGGAGAA ACAGCTATAA GAGCCGGATG
    GTGAGGCGGA GCAAGAAATC TAAGAAGAAG GAAAGTCTGG AAAGGAGGAG ATCCCTTTTC
    AAAAAACTAG CTAAGCAGCC TTCTCCTTTA CTCCACACCA GCCGAAGTTT CTCCTGCTTG
    AACCGATCCC TGTCGTCAGG GGAGAGCCTC CCAGGGTCCC CCACTCACAG CTTGTCTCCC
    CGATCCCCTA CACCCAGCTA CCGTTCCACC CCCGACTTCC CATCCGGCAC TAACTCCTCC
    CAGAGCAGCT CCCCAAGCTC CAGCGCTCCC AACTCCCCAG CAGGGTCAGG ACACGTCCGG
    CCCAGCACGC TCCATGGTCT GGCGCCCAAA CTCAGCGGGC AGCGCTACCG GTCAGGAAGG
    CGGAAGTCAG CTGGCAGCAT CCCCCTGTCT CCGCTGGCCC GGACACCCTC TCCGACCCCA
    CAGCCCACCT CCCCGCAGCG GTCCCCATCC CCTCTGCTGG GGCACTCACT TGGCAATTCC
    AAGATGGCTC AAGCCTTTCC CAGCAAAATG CACTCCCCAC CCACCATCGT CAGGCACGTC
    GTGAGGCCCA AGAGTGCAGA ACCCCCTCGG TCCCCGCTGC TCAAGCGCGT GCAGTCGGAG
    GAGAAGCTGT CTCCCTCCTA TGGAGGCGAC AAGAAGCACC TGTGCTCCCG CAAGCACAGC
    CTTGAGGTCA CCCAGGAGGA GGTGCCGCGG GAGCAGCAAC AGCGGGAGGT GACCTTGCAG
    AGCCTGGAGG AGAACGTGTG CGACGCGCCT GCCCTCAGCC GGGCCAGGCC CGTGGAGCAG
    GGCTGCCTGA AACGCCCCGT GTCCCGGAAG CTGGGCCGCC AGGAGTCTGT GGACGAGCTG
    GACCGAGAGA AGCTAAAGGC CAAGGTGGTG GTGAAGAAAC CTGACGGGCT CTCGGAAAAA
    CAGGAATCCC GCCCGAAGCC CCACGGACTC GGCAGTGACT TGGAAAACCT CTGTGCTTTT
    AGGCTCGAAG AGAGAGACAA GAAAATCTAT CCAAAAGCTT CAGAGAGGTC AAATCATTTT
    GAAAGCAAAG CAGCCGTGCA GGAGGCCCAG CCCCTGTGCA GCCTTTTGAA AGATGCTCTC
    CACAAGCAGG CCAATGTGCG GGCCAGCGAG GGTGTCGCCT CTGAGGGGGC AACGGCAGCT
    GGGGACCACG GCCAGGGCAC CTGTGACTTC AAACGAGCCT CAACGCACAG CACCCTCCAA
    GACGGCCTCT GTCACACCAC CCACAGGTCC ACCTCCGGGA AGGGGGACAC CGCAGAGAAG
    GCCCCCCAGG CCAAGGAGGG TCTGCGGTGT GAGAAGTTAG ACGGCAAGTT GGCCAACATC
    GATTACCTCC GAAAGAAGAT GTCCCTTGAA GACAAAGACG AGAGCCTCTG TTCTGTGCTC
    AAGCCCAAGG TGACAGCTGG TGCCCAGGAC TGCCTGCCTG GGAGCCTGGG CCGGCCCGTG
    GGAGGGCAGC AGGAGGCCCC GCCAGCCTCC GAGGCCCGCA CACCGTTCAT CGGCAGCGCC
    CACGCGGCTC AGCTCAGCGC CGTCTCCTTT GTCCCTCTCA AGGCCCTTTC TGGCCGGGTG
    GACAGTGGAG CGGAGAAGCC GGGCTTGGCT GCCCCAGAGT CGCCTGTCCG GAAAAGCCCC
    TCCGAGTATA AGCTGGAAGG GCGGTCTGTG TCGTGCCTGA AGCCCATTGA AGGCACCTTG
    GACATCGCTC TCCTGTCGGG ACCTCAGGCC TCCAAGACAG AGCTGCCTTC CCCGGAGCCT
    GCCCAGAGCC CCAGCCCAGG CAGTGATGTG GAGTCCCCCA TGCCCCTCGC TCTCCCCAGC
    AGCACAGGGA GGAAGGCTGA CACTGCGGGT CAGAGAGAGT CCTCCCCTGC CGGCTTCAAG
    GTGAACAAAT CCTACCTGCT TGAGCCCCGG TTCCCACCGG CCAGCCGGGG TCTGCAGAGT
    TCACCGGTGG TTGCCCCGCC TGACCCAGAG CTGAAGCTGG ACAAGAAGGT CTCCCAGGCA
    GCCCGTGCCC CAGCGACCAT CATGGAGAGC CATCCCCAGC GGGGAGAGGG CCACCCCAGC
    CAACACCAAG ACCACAGCCC CGACGTTAAG CTCCTTCCCT TCCCAGGGCA GAACCTCCAA
    GGTGCCAACA CGTCTAGATC CCGCAGCCCA CTCTTGCCTG AAAGTGTCCC CCCTAGAGAG
    AAGCCGAGTG GGAGAGAGTT GTCCGAAAGG GGCCCTTCCA TAGTCGGGAG CGAGTGGTCA
    GCTGTGAGGC CCGACATCCG CAGAGACCCC TCCAAGGAGC TGTGTCCCCC AGAAGCTGCT
    AAACCCAGCG ACAACTCCCA AACCCCCCCT GTGGTGGGGA GGACCCGCCT GGATGTCTCC
    ACCCAGACCC AGGCCTTGGA GAAAGCATGC GGGCCTTGCG GGAGAGCAAA GCCCAGAGAC
    GGCCGAGAGG AGGTGAGGCT GCCGGCCAGA GAGGACTCCT CTTTGCGCTC TGCGGGGGCT
    CCTTGTGAGA GAGAGCTGGG CGCAGAGAGA GGCCTGGAAT CCAAGATGGA ACCTGCTTCT
    ACCAGGTGGT CTCTGGAGCC GCCCAGGCCA GAGAGTGAGA AGAGTGGAAG GCCTTCTGGT
    TTCCAGTCTG TGCAGAAAGA CGGTCCCAAG GAACCGGAGA GGAAAGAACA ACCTCTGCAA
    AAGTACCTCA CCAGTTCTCA GCCGGGTCCC AAGGAACCCG AGAGGAAAGA ACAGCCCCTG
    CAAAAGCACC TCCCCAGTAG TTCTCAGCCG GGTCCCAAGG AACCCGAGAG GAAAGAACAG
    CCCCTGCAAA AGCACGTCAC CAGTAGTTCT CAGCCGGGTC CCAAGGAACC GGAGAGGAAA
    GAACAGCCTC TGCAAAAGCA CCTCCCCAGT AGTTCTCAGC CGGGTCCCAA GGAACCGGAG
    AGGAAAGAAC AGCCCCTGCA AAAGCACGTC ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA
    CCCGAGAGGA AAGAACAGCC TCTGCAAAAG CACCTCCCCA GTAGTTCTCA GCCGGGTCCC
    AAGGAACTGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACC TCACCAGTAG CTCTCAGCTG
    GGTCCCAAGG AACCAGAAAT GAAAGAACAG CCCCCACAAA AGCACGTCAC CAGTAGTTCT
    CAACTGTGTC CCAAGGAACT GGAAAGGAAA GAACAACCTC CACAAAAGCA CCTCACTATT
    AGCTCTCAGC TGAGTCCCAA GGAACCAGAA AGGAAAGAAC AGCCTCTGCA AGAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA CCGGAAAGGA AAGAACAGTG TTTGCAGAAG
    CACTTCACCA GTAGTTCTCA GCTGGGTCCC AAGGAACCAG AAACGAAAGA ACAGCCTCTG
    CAAAAGCACC TCACCAGTAG CTCTCAGCTG AGTCCCAAGG AACCGGAAAG GAAAGAACAG
    CTTCTGCAAG AGCACCTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAACC GGAAAGGAAA
    GAACAGTGTT TGCAGAAGCA CCTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCAGAA
    ATGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA
    CCAGAAAGGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGCCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACC TCACCAGTAG CTGTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGCC CCAAGGAGCC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CCTCACCATT
    AGCTCTCAGC TGAGTCCCAA GGAACCAGAA AGGAAAGAAC AGCCTCTGCA AAAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG CCAGAAAGGA AAGAACAGCT TCTGCAAAAG
    CACCTCACCA TTAGCTCTCA GCTGAGTCCC AAGGAGCCGG AAAGGAAAGA ACAGCTTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AGCCGGAAAA GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CCTCACCATT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCCTCTGCA AAAGCACGTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAGGA AAGAACAGCT TCTGCAAAAG CACCTCACCA TTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACG TCACCAGTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC GGAAAGGAAA GAACAGTATT TGCAGAAGCA CCTCACCAGT
    AGTTCTCAGC TGGGTCCCAA GGAACCAGAA ACGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCAGTAGTT CTCAGGTGGG TCCCAAGGAT CCGGAAAGGA AAGAACAGCC CCCACAAAAG
    CACCTCACCA GTAAGTCTCA GCCGGGTCCC AAGGAACCGG AAAGGAAAGA ACAGTGTTTG
    CAAAAGCACC TCACCAGTAG TTCTCAGCCG GGTCCCAAGG AACTGGAAAG GAAAGAACAA
    CCTCCACTAA AGCATGTCAC CAGTAGCTCT CAGCTGGGTC CCAGGGAACC AGAAAGGAAA
    GAACAGCCCC CACAAAAGCA CCTCACCAGT AGCTGTCAGC CCCCACCCCC TACCAAAGAG
    CTCCCTGGCC TGGCTTGTCA GCACTGCATT CTGCCCAGCC ACCCTGCTGC CTCTCTGCTG
    GGGAGCAAGC CCTGTGCTAT AGACTCCAGC TTCCAGGATG CTCTCAGGCC CACTGCTGTG
    CGCAGTGACA GCAGCAGCCA CAAGCCCAGG TCTGACCCCG ACCTGGGCCC TCCAAAGTCT
    AAGTTCCCAG ACCGGCCCCT CTCCTCCCAG AAGCCGAGCG CTGAGGCTGC AGTGAGCAAA
    GAGCCTGGCG CTCCGACCCT CGGCAGAGCT GGGGTGGGGA GTGGTAAGGG TGTGTCAGAG
    GAGTTCCCTG TCCTTCCAGA TCCCCAGAAC ACAGCCCGTG AAGTGATTGG CCAGGGAGCC
    AGTGGGTACT CAGGCCCCCT GCCCATGGAA GGGGGTCCTC TAGACACAAA GCTGAGACCC
    ACCAGCGCTC GGTGTCCCCC AGAGGCACTG GAGATGCTTG GACATCCACC AGGGCGAGGG
    CTCCTAGGGG CCAGTGAGTC AGTGGACCAG AAACTCCCCA CCTTCAGGGA AAGGCAAAAC
    CTGTCTCCAA AGGCCCCCAC ACCATCCACT GTGAAAGATT GTCCCCTTCC GTGCAAACAG
    ACAGACAGGA GCCCGAGTCC TCTGGCCACC AGCACCAACG CTGGCAAGTC TGAAGGCAAG
    AAATGCACTG AAGCACTTTA CACCCCTGCG GATGGTGAGA AGCTGGGGGC CGGCTTGTCC
    TCGGTGCACA GCGAGGCTGG GCCTAAGGGC ACAGAGAAGC CGGCAGCAGC CGCTGGGAAG
    GGCTGGCCAG AGGCCAAGGG GAAAGGACTC AGTCCCCAGA AGCCACTGAC CGAGGCCAGC
    AAGCCCAGCG GCATGAAGCG GTCACCTTCA GCCACGGTGC AGAGCTCTCT CCGCTGTGCC
    ACCCTCCCAG AGAAGTCTCT GAGCTACTCC TGTGGCTTTC CCGAGGCCAG ACCAGCAGTT
    CCAGAGACTT CTACCACCTC CAGCGACACC TCCTCTGCCA AGGCTGCTGG GCCTGCAGCA
    GAATCCCCAG CCTCCAGCAG CAGGGACCAG CAGAAACCCC TGCCTGGTGG GGATGGCAGA
    ACCCAAATGA CCAAGAGCGA CTCCTTGCCA TGCTTCCGCA GCTCAGCCAT CACTCTGGAG
    TCCCAGCACC CCAGCCCTGG CCTGGTGGTG GTCACCAGCC ACCGGGACAG GGCCCTCTCA
    GTAACCGCCA CCGTGGGAGA AACCAAAGGG AAAGAGCCAG CCCCCACCCA GCCAGCTCAG
    ACCAGGAACC AGAACGTGAG CAGAGAGGCG GCCAAGCCGT CCCCCGCTCC AAGCACTGAG
    CGCCCCATCG TTCTTCCTTC TGAGAAGGAC ACTGGGCTGC GACAGAGGCG GGGGAAAGAG
    AGTTTGCGCG GCAGCCCCCA CAAAAAGGCC TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837213.1
    CDS263..10258
    Translation

    Target ORF information:

    RefSeq Version XM_024981445.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC ACCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GAAGGAGCTG AGTCTTCCCA GAAGAGGAAG TCTTTGCCGA
    ACCAGCAACC GGAAAAGCTT GATAGGCAAT GGACCATCCC CAGCATTGCC TCGGCCCCAC
    TCACCTCTTT CTGCTCATGC AGGAAATAGC CCTCAAGATA GTCCAAGAAA TTTCTCCCCC
    AGTGCCTCTG CTCATTTTTC ATTTGCACGG AGGACTGATG GACGCCGCTG GTCCTTGGCT
    TCTCTCCCCT CCTCTGGCTA TGGGACAAAC ACTCCCAGCT CTACGGTCTC TTCATCCTGT
    TCCTCCCAGG AGAAGTTGCA TCAGTTACCG TATCAACCGA CACCAGATGA ATTACACTTC
    TTATCAAAAC ATTTTTGTAC CACTGAAAGC ATCACCACAG AGAACAGATG CAGAAACACA
    CCCATGCGCC CCCGTTCCCG AAGTTTGAGC CCTGGACGTT CTCCCGCCTG CTGTGACCAT
    GAAATAATTA TGATGAACCA TGTCTACAAA GAAAGGTTCC CAAAGGCTAC AGCTCAGATG
    GAAGAGCGCC TAAAGGAGAT CATCACCAGC TACTCTCCAG ACCACGTTCT CCCCCTTGCA
    GATGGAGTGC TCAGCTTCAC TCACCATCAG ATTATCGAAC TGGCTCGCGA TTGTTTGGAT
    AAATCCCACC AGGGTCTCAT CACCTCACGA TACTTCCTTG AATTACAGCA CAAATTAGAT
    AAGTTACTAC AGGAGGCTCA CAATCGTTCA GAAAGTGGAG AGCTGGCATT TATTAAACAA
    CTAGTTCGAA AGATCCTGAT TGTTATTGCC CGCCCTGCTC GCTTATTAGA GTGCCTGGAA
    TTTGATCCTG AAGAATTTTA CTACCTATTG GAAGCAGCAG AAGGCCATGC CAAAGAAGGA
    CAGGGTATTA AAACTGACAT TCCCAGGTAC ATCATTAGCC AGCTTGGCCT CAATAAAGAT
    CCTCTGGAAG AAATGGCTCA ACTGGGAAAC TATGATAGTG GGACAGCAGA AACACCAGAA
    ACAGATGAAT CAGTGAGTAG CTCTAATGCT TCCCTGAAAC TTCGAAGGAA GCCTCGGGAA
    AGTGATTTTG AAACGATTAA ATTGATCAGC AATGGAGCCT ACGGGGCAGT CTATTTCGTT
    CGGCATAAGG AATCCCGGCA GAGGTTTGCC ATGAAGAAGA TTAACAAACA GAACCTCATC
    CTCCGAAACC AGATCCAGCA GGCCTTTGTG GAACGGGATA TCCTGACTTT TGCAGAAAAC
    CCTTTTGTCG TCAGCATGTA CTGCTCCTTT GAGACCAGGC GCCACTTGTG CATGGTCATG
    GAGTATGTGG AAGGGGGTGA CTGTGCCACG TTAATGAAGA ACATGGGTCC TCTCCCCGTA
    GATATGGCCA GGATGTACTT TGCTGAGACG GTCCTGGCCT TGGAATATCT ACATAATTAT
    GGAATTGTAC ATAGGGATTT GAAACCAGAC AATTTGCTGG TCACCTCTAT GGGGCACATA
    AAGCTGACCG ATTTTGGATT ATCCAAGGTG GGACTCATGA GCATGACTAC CAATCTTTAT
    GAGGGTCATA TTGAGAAGGA TGCCCGAGAG TTCCTGGACA AGCAGGTCTG TGGCACACCT
    GAATACATTG CACCAGAAGT GATTCTGAGG CAGGGCTACG GGAAGCCAGT GGACTGGTGG
    GCCATGGGGA TTATCCTCTA TGAATTTCTG GTTGGATGCG TGCCATTCTT TGGGGACACT
    CCAGAAGAGC TATTTGGACA AGTCATCAGT GATGAGATCA ACTGGCCTGA AAAGGATGAG
    GCTCCACCCC CTGATGCCCA GGATCTGATT ACCTTGCTCC TCAGGCAGAA TCCCCTGGAG
    AGACTGGGAA CAGGTGGTGC ATATGAAGTC AAACAGCATC GATTCTTCCG TTGCTTAGAC
    TGGAACAGTT TGCTGAGACA GAAGGCAGAA TTCATTCCCC AACTGGAATC TGAAGACGAC
    ACAAGTTATT TTGATACTCG GTCAGAGAAG TACCATCATA TGGAGACTGA GGAAGAAGAT
    GACACGAACG ATGAAGACTT TAATGTGGAA ATAAGGCAGT TTTCCTCATG TTCACATAGG
    TTTTCTAAGG TCTTCAGCAG TATGGATCGA GGTACTCAGA ATTCAGGAGA AGAGAAGGAA
    GACCCTGGGG ACAAAACTAA AAGCACAGCT TTGCCGTCCA CGGAAACACT AAGCTGGAGT
    TCAGAGTATT CTGAAATGCA ACAGTTATCC ACATCCAACT CTTCAGACAC TGAAAGCAAC
    AGACATAGAC GCGGTGCTGG GCTGCTTCCC AAGCTGGCTG TTTCAGCAGA GGCAGAACAC
    GACGGGGCCG CTCCCCGCCC CAGAGAGCTG CACGAGGAGC CGGAAAAGCC AGCCCTGCCT
    GCTGCAGAGA ATGCTCAGGA GGAGCCCGAG GTCACTACCC CGGCCAGCAC CATCAGCAGC
    TCCACTCTGT CAGTTGGCAG TTTTTCAGAG CACTTGGATC AGATAAATGG ACGGAGCGAG
    TGTGTGGACA GTACAGACAA TGCCTCCAAG CCCTCCAATG AGCCCGCCTC TCACATGGCT
    CGGCAGCGCT TAGAAAGCAC AGAGAAACAG AAAGCCTCGG GGAAAGTCAC AAAGTCCCTT
    TCTGCCAGTG CCCTGTCCCT CATGATCCCA GGAGATATGT TTGCTGTCTC TCCGCTGGGA
    AGTCCGATGT CTCCCCATTC CCTGTCCTCG GACCCTTCTT CCTCAAGGGA TTCCTCTCCC
    AGCCGAGATT CTTCAGGAGC TTCTGCCAGT CCACACCAGC CCATTGTGAT CCACAGTTTG
    GGGAAGAACT ATGGTTTCAC CATTCGAGCC ATCCGGGTGT ACGTGGGAGA CAGCGACATT
    TACACAGTGC ATCATATCGT TTGGAATGTA GAAGAAGGAA GTCCAGCTTG CCAGGCAGGA
    CTGAAAGCTG GGGATCTGAT CACACACATC AATGGAGAAC CGGTGCACGG ACTTGTCCAC
    ACAGAAGTTA TAGAGCTCCT GCTAAAGAGT GGAAATAAGG TGTCAATCAC GACCACCCCA
    TTTGAAAACA CATCAATCAA AACGGGACCG GCCAGGAGAA ACAGCTATAA GAGCCGGATG
    GTGAGGCGGA GCAAGAAATC TAAGAAGAAG GAAAGTCTGG AAAGGAGGAG ATCCCTTTTC
    AAAAAACTAG CTAAGCAGCC TTCTCCTTTA CTCCACACCA GCCGAAGTTT CTCCTGCTTG
    AACCGATCCC TGTCGTCAGG GGAGAGCCTC CCAGGGTCCC CCACTCACAG CTTGTCTCCC
    CGATCCCCTA CACCCAGCTA CCGTTCCACC CCCGACTTCC CATCCGGCAC TAACTCCTCC
    CAGAGCAGCT CCCCAAGCTC CAGCGCTCCC AACTCCCCAG CAGGGTCAGG ACACGTCCGG
    CCCAGCACGC TCCATGGTCT GGCGCCCAAA CTCAGCGGGC AGCGCTACCG GTCAGGAAGG
    CGGAAGTCAG CTGGCAGCAT CCCCCTGTCT CCGCTGGCCC GGACACCCTC TCCGACCCCA
    CAGCCCACCT CCCCGCAGCG GTCCCCATCC CCTCTGCTGG GGCACTCACT TGGCAATTCC
    AAGATGGCTC AAGCCTTTCC CAGCAAAATG CACTCCCCAC CCACCATCGT CAGGCACGTC
    GTGAGGCCCA AGAGTGCAGA ACCCCCTCGG TCCCCGCTGC TCAAGCGCGT GCAGTCGGAG
    GAGAAGCTGT CTCCCTCCTA TGGAGGCGAC AAGAAGCACC TGTGCTCCCG CAAGCACAGC
    CTTGAGGTCA CCCAGGAGGA GGTGCCGCGG GAGCAGCAAC AGCGGGAGGT GACCTTGCAG
    AGCCTGGAGG AGAACGTGTG CGACGCGCCT GCCCTCAGCC GGGCCAGGCC CGTGGAGCAG
    GGCTGCCTGA AACGCCCCGT GTCCCGGAAG CTGGGCCGCC AGGAGTCTGT GGACGAGCTG
    GACCGAGAGA AGCTAAAGGC CAAGGTGGTG GTGAAGAAAC CTGACGGGCT CTCGGAAAAA
    CAGGAATCCC GCCCGAAGCC CCACGGACTC GGCAGTGACT TGGAAAACCT CTGTGCTTTT
    AGGCTCGAAG AGAGAGACAA GAAAATCTAT CCAAAAGCTT CAGAGAGGTC AAATCATTTT
    GAAAGCAAAG CAGCCGTGCA GGAGGCCCAG CCCCTGTGCA GCCTTTTGAA AGATGCTCTC
    CACAAGCAGG CCAATGTGCG GGCCAGCGAG GGTGTCGCCT CTGAGGGGGC AACGGCAGCT
    GGGGACCACG GCCAGGGCAC CTGTGACTTC AAACGAGCCT CAACGCACAG CACCCTCCAA
    GACGGCCTCT GTCACACCAC CCACAGGTCC ACCTCCGGGA AGGGGGACAC CGCAGAGAAG
    GCCCCCCAGG CCAAGGAGGG TCTGCGGTGT GAGAAGTTAG ACGGCAAGTT GGCCAACATC
    GATTACCTCC GAAAGAAGAT GTCCCTTGAA GACAAAGACG AGAGCCTCTG TTCTGTGCTC
    AAGCCCAAGG TGACAGCTGG TGCCCAGGAC TGCCTGCCTG GGAGCCTGGG CCGGCCCGTG
    GGAGGGCAGC AGGAGGCCCC GCCAGCCTCC GAGGCCCGCA CACCGTTCAT CGGCAGCGCC
    CACGCGGCTC AGCTCAGCGC CGTCTCCTTT GTCCCTCTCA AGGCCCTTTC TGGCCGGGTG
    GACAGTGGAG CGGAGAAGCC GGGCTTGGCT GCCCCAGAGT CGCCTGTCCG GAAAAGCCCC
    TCCGAGTATA AGCTGGAAGG GCGGTCTGTG TCGTGCCTGA AGCCCATTGA AGGCACCTTG
    GACATCGCTC TCCTGTCGGG ACCTCAGGCC TCCAAGACAG AGCTGCCTTC CCCGGAGCCT
    GCCCAGAGCC CCAGCCCAGG CAGTGATGTG GAGTCCCCCA TGCCCCTCGC TCTCCCCAGC
    AGCACAGGGA GGAAGGCTGA CACTGCGGGT CAGAGAGAGT CCTCCCCTGC CGGCTTCAAG
    GTGAACAAAT CCTACCTGCT TGAGCCCCGG TTCCCACCGG CCAGCCGGGG TCTGCAGAGT
    TCACCGGTGG TTGCCCCGCC TGACCCAGAG CTGAAGCTGG ACAAGAAGGT CTCCCAGGCA
    GCCCGTGCCC CAGCGACCAT CATGGAGAGC CATCCCCAGC GGGGAGAGGG CCACCCCAGC
    CAACACCAAG ACCACAGCCC CGACGTTAAG CTCCTTCCCT TCCCAGGGCA GAACCTCCAA
    GGTGCCAACA CGTCTAGATC CCGCAGCCCA CTCTTGCCTG AAAGTGTCCC CCCTAGAGAG
    AAGCCGAGTG GGAGAGAGTT GTCCGAAAGG GGCCCTTCCA TAGTCGGGAG CGAGTGGTCA
    GCTGTGAGGC CCGACATCCG CAGAGACCCC TCCAAGGAGC TGTGTCCCCC AGAAGCTGCT
    AAACCCAGCG ACAACTCCCA AACCCCCCCT GTGGTGGGGA GGACCCGCCT GGATGTCTCC
    ACCCAGACCC AGGCCTTGGA GAAAGCATGC GGGCCTTGCG GGAGAGCAAA GCCCAGAGAC
    GGCCGAGAGG AGGTGAGGCT GCCGGCCAGA GAGGACTCCT CTTTGCGCTC TGCGGGGGCT
    CCTTGTGAGA GAGAGCTGGG CGCAGAGAGA GGCCTGGAAT CCAAGATGGA ACCTGCTTCT
    ACCAGGTGGT CTCTGGAGCC GCCCAGGCCA GAGAGTGAGA AGAGTGGAAG GCCTTCTGGT
    TTCCAGTCTG TGCAGAAAGA CGGTCCCAAG GAACCGGAGA GGAAAGAACA ACCTCTGCAA
    AAGTACCTCA CCAGTTCTCA GCCGGGTCCC AAGGAACCCG AGAGGAAAGA ACAGCCCCTG
    CAAAAGCACC TCCCCAGTAG TTCTCAGCCG GGTCCCAAGG AACCCGAGAG GAAAGAACAG
    CCCCTGCAAA AGCACGTCAC CAGTAGTTCT CAGCCGGGTC CCAAGGAACC GGAGAGGAAA
    GAACAGCCTC TGCAAAAGCA CCTCCCCAGT AGTTCTCAGC CGGGTCCCAA GGAACCGGAG
    AGGAAAGAAC AGCCCCTGCA AAAGCACGTC ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA
    CCCGAGAGGA AAGAACAGCC TCTGCAAAAG CACCTCCCCA GTAGTTCTCA GCCGGGTCCC
    AAGGAACTGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACC TCACCAGTAG CTCTCAGCTG
    GGTCCCAAGG AACCAGAAAT GAAAGAACAG CCCCCACAAA AGCACGTCAC CAGTAGTTCT
    CAACTGTGTC CCAAGGAACT GGAAAGGAAA GAACAACCTC CACAAAAGCA CCTCACTATT
    AGCTCTCAGC TGAGTCCCAA GGAACCAGAA AGGAAAGAAC AGCCTCTGCA AGAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA CCGGAAAGGA AAGAACAGTG TTTGCAGAAG
    CACTTCACCA GTAGTTCTCA GCTGGGTCCC AAGGAACCAG AAACGAAAGA ACAGCCTCTG
    CAAAAGCACC TCACCAGTAG CTCTCAGCTG AGTCCCAAGG AACCGGAAAG GAAAGAACAG
    CTTCTGCAAG AGCACCTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAACC GGAAAGGAAA
    GAACAGTGTT TGCAGAAGCA CCTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCAGAA
    ATGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA
    CCAGAAAGGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGCCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACC TCACCAGTAG CTGTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGCC CCAAGGAGCC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CCTCACCATT
    AGCTCTCAGC TGAGTCCCAA GGAACCAGAA AGGAAAGAAC AGCCTCTGCA AAAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG CCAGAAAGGA AAGAACAGCT TCTGCAAAAG
    CACCTCACCA TTAGCTCTCA GCTGAGTCCC AAGGAGCCGG AAAGGAAAGA ACAGCTTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AGCCGGAAAA GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CCTCACCATT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCCTCTGCA AAAGCACGTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAGGA AAGAACAGCT TCTGCAAAAG CACCTCACCA TTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACG TCACCAGTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC GGAAAGGAAA GAACAGTATT TGCAGAAGCA CCTCACCAGT
    AGTTCTCAGC TGGGTCCCAA GGAACCAGAA ACGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCAGTAGTT CTCAGGTGGG TCCCAAGGAT CCGGAAAGGA AAGAACAGCC CCCACAAAAG
    CACCTCACCA GTAAGTCTCA GCCGGGTCCC AAGGAACCGG AAAGGAAAGA ACAGTGTTTG
    CAAAAGCACC TCACCAGTAG TTCTCAGCCG GGTCCCAAGG AACTGGAAAG GAAAGAACAA
    CCTCCACTAA AGCATGTCAC CAGTAGCTCT CAGCTGGGTC CCAGGGAACC AGAAAGGAAA
    GAACAGCCCC CACAAAAGCA CCTCACCAGT AGCTGTCAGC CCCCACCCCC TACCAAAGAG
    CTCCCTGGCC TGGCTTGTCA GCACTGCATT CTGCCCAGCC ACCCTGCTGC CTCTCTGCTG
    GGGAGCAAGC CCTGTGCTAT AGACTCCAGC TTCCAGGATG CTCTCAGGCC CACTGCTGTG
    CGCAGTGACA GCAGCAGCCA CAAGCCCAGG TCTGACCCCG ACCTGGGCCC TCCAAAGTCT
    AAGTTCCCAG ACCGGCCCCT CTCCTCCCAG AAGCCGAGCG CTGAGGCTGC AGTGAGCAAA
    GAGCCTGGCG CTCCGACCCT CGGCAGAGCT GGGGTGGGGA GTGGTAAGGG TGTGTCAGAG
    GAGTTCCCTG TCCTTCCAGA TCCCCAGAAC ACAGCCCGTG AAGTGATTGG CCAGGGAGCC
    AGTGGGTACT CAGGCCCCCT GCCCATGGAA GGGGGTCCTC TAGACACAAA GCTGAGACCC
    ACCAGCGCTC GGTGTCCCCC AGAGGCACTG GAGATGCTTG GACATCCACC AGGGCGAGGG
    CTCCTAGGGG CCAGTGAGTC AGTGGACCAG AAACTCCCCA CCTTCAGGGA AAGGCAAAAC
    CTGTCTCCAA AGGCCCCCAC ACCATCCACT GTGAAAGATT GTCCCCTTCC GTGCAAACAG
    ACAGACAGGA GCCCGAGTCC TCTGGCCACC AGCACCAACG CTGGCAAGTC TGAAGGCAAG
    AAATGCACTG AAGCACTTTA CACCCCTGCG GATGGTGAGA AGCTGGGGGC CGGCTTGTCC
    TCGGTGCACA GCGAGGCTGG GCCTAAGGGC ACAGAGAAGC CGGCAGCAGC CGCTGGGAAG
    GGCTGGCCAG AGGCCAAGGG GAAAGGACTC AGTCCCCAGA AGCCACTGAC CGAGGCCAGC
    AAGCCCAGCG GCATGAAGCG GTCACCTTCA GCCACGGTGC AGAGCTCTCT CCGCTGTGCC
    ACCCTCCCAG AGAAGTCTCT GAGCTACTCC TGTGGCTTTC CCGAGGCCAG ACCAGCAGTT
    CCAGAGACTT CTACCACCTC CAGCGACACC TCCTCTGCCA AGGCTGCTGG GCCTGCAGCA
    GAATCCCCAG CCTCCAGCAG CAGGGACCAG CAGAAACCCC TGCCTGGTGG GGATGGCAGA
    ACCCAAATGA CCAAGAGCGA CTCCTTGCCA TGCTTCCGCA GCTCAGCCAT CACTCTGGAG
    TCCCAGCACC CCAGCCCTGG CCTGGTGGTG GTCACCAGCC ACCGGGACAG GGCCCTCTCA
    GTAACCGCCA CCGTGGGAGA AACCAAAGGG AAAGAGCCAG CCCCCACCCA GCCAGCTCAG
    ACCAGGAACC AGAACGTGAG CAGAGAGGCG GCCAAGCCGT CCCCCGCTCC AAGCACTGAG
    CGCCCCATCG TTCTTCCTTC TGAGAAGGAC ACTGGGCTGC GACAGAGGCG GGGGAAAGAG
    AGTTTGCGCG GCAGCCCCCA CAAAAAGGCC TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228112
    Clone ID Related Accession (Same CDS sequence) XM_024981446.1
    Accession Version XM_024981446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
    1381
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    1501
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    1981
    2041
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    2641
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    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC ACCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GAAGGAGCTG AGTCTTCCCA GAAGAGGAAG TCTGATAGAT
    TCCCAGAAGT GGAATTGCTT GGTCAAACGT TGCCGAACCA GCAACCGGAA AAGCTTGATA
    GGCAATGGAC CATCCCCAGC ATTGCCTCGG CCCCACTCAC CTCTTTCTGC TCATGCAGGA
    AATAGCCCTC AAGATAGTCC AAGAAATTTC TCCCCCAGTG CCTCTGCTCA TTTTTCATTT
    GCACGGAGGA CTGATGGACG CCGCTGGTCC TTGGCTTCTC TCCCCTCCTC TGGCTATGGG
    ACAAACACTC CCAGCTCTAC GGTCTCTTCA TCCTGTTCCT CCCAGGAGAA GTTGCATCAG
    TTACCGTATC AACCGACACC AGATGAATTA CACTTCTTAT CAAAACATTT TTGTACCACT
    GAAAGCATCA CCACAGAGAA CAGATGCAGA AACACACCCA TGCGCCCCCG TTCCCGAAGT
    TTGAGCCCTG GACGTTCTCC CGCCTGCTGT GACCATGAAA TAATTATGAT GAACCATGTC
    TACAAAGAAA GGTTCCCAAA GGCTACAGCT CAGATGGAAG AGCGCCTAAA GGAGATCATC
    ACCAGCTACT CTCCAGACCA CGTTCTCCCC CTTGCAGATG GAGTGCTCAG CTTCACTCAC
    CATCAGATTA TCGAACTGGC TCGCGATTGT TTGGATAAAT CCCACCAGGG TCTCATCACC
    TCACGATACT TCCTTGAATT ACAGCACAAA TTAGATAAGT TACTACAGGA GGCTCACAAT
    CGTTCAGAAA GTGGAGAGCT GGCATTTATT AAACAACTAG TTCGAAAGAT CCTGATTGTT
    ATTGCCCGCC CTGCTCGCTT ATTAGAGTGC CTGGAATTTG ATCCTGAAGA ATTTTACTAC
    CTATTGGAAG CAGCAGAAGG CCATGCCAAA GAAGGACAGG GTATTAAAAC TGACATTCCC
    AGGTACATCA TTAGCCAGCT TGGCCTCAAT AAAGATCCTC TGGAAGAAAT GGCTCAACTG
    GGAAACTATG ATAGTGGGAC AGCAGAAACA CCAGAAACAG ATGAATCAGT GAGTAGCTCT
    AATGCTTCCC TGAAACTTCG AAGGAAGCCT CGGGAAAGTG ATTTTGAAAC GATTAAATTG
    ATCAGCAATG GAGCCTACGG GGCAGTCTAT TTCGTTCGGC ATAAGGAATC CCGGCAGAGG
    TTTGCCATGA AGAAGATTAA CAAACAGAAC CTCATCCTCC GAAACCAGAT CCAGCAGGCC
    TTTGTGGAAC GGGATATCCT GACTTTTGCA GAAAACCCTT TTGTCGTCAG CATGTACTGC
    TCCTTTGAGA CCAGGCGCCA CTTGTGCATG GTCATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACGTTAA TGAAGAACAT GGGTCCTCTC CCCGTAGATA TGGCCAGGAT GTACTTTGCT
    GAGACGGTCC TGGCCTTGGA ATATCTACAT AATTATGGAA TTGTACATAG GGATTTGAAA
    CCAGACAATT TGCTGGTCAC CTCTATGGGG CACATAAAGC TGACCGATTT TGGATTATCC
    AAGGTGGGAC TCATGAGCAT GACTACCAAT CTTTATGAGG GTCATATTGA GAAGGATGCC
    CGAGAGTTCC TGGACAAGCA GGTCTGTGGC ACACCTGAAT ACATTGCACC AGAAGTGATT
    CTGAGGCAGG GCTACGGGAA GCCAGTGGAC TGGTGGGCCA TGGGGATTAT CCTCTATGAA
    TTTCTGGTTG GATGCGTGCC ATTCTTTGGG GACACTCCAG AAGAGCTATT TGGACAAGTC
    ATCAGTGATG AGATCAACTG GCCTGAAAAG GATGAGGCTC CACCCCCTGA TGCCCAGGAT
    CTGATTACCT TGCTCCTCAG GCAGAATCCC CTGGAGAGAC TGGGAACAGG TGGTGCATAT
    GAAGTCAAAC AGCATCGATT CTTCCGTTGC TTAGACTGGA ACAGTTTGCT GAGACAGAAG
    GCAGAATTCA TTCCCCAACT GGAATCTGAA GACGACACAA GTTATTTTGA TACTCGGTCA
    GAGAAGTACC ATCATATGGA GACTGAGGAA GAAGATGACA CGAACGATGA AGACTTTAAT
    GTGGAAATAA GGCAGTTTTC CTCATGTTCA CATAGGTTTT CTAAGGTCTT CAGCAGTATG
    GATCGAGGTA CTCAGAATTC AGGAGAAGAG AAGGAAGACC CTGGGGACAA AACTAAAAGC
    ACAGCTTTGC CGTCCACGGA AACACTAAGC TGGAGTTCAG AGTATTCTGA AATGCAACAG
    TTATCCACAT CCAACTCTTC AGACACTGAA AGCAACAGAC ATAGACGCGG TGCTGGGCTG
    CTTCCCAAGC TGGCTGTTTC AGCAGAGGCA GAACACGACG GGGCCGCTCC CCGCCCCAGA
    GAGCTGCACG AGGAGCCGGA AAAGCCAGCC CTGCCTGCTG CAGAGAATGC TCAGGAGGAG
    CCCGAGGTCA CTACCCCGGC CAGCACCATC AGCAGCTCCA CTCTGTCAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCGGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTATTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAACGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGTTCTCAG GTGGGTCCCA AGGATCCGGA AAGGAAAGAA
    CAGCCCCCAC AAAAGCACCT CACCAGTAAG TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG
    AAAGAACAGT GTTTGCAAAA GCACCTCACC AGTAGTTCTC AGCCGGGTCC CAAGGAACTG
    GAAAGGAAAG AACAACCTCC ACTAAAGCAT GTCACCAGTA GCTCTCAGCT GGGTCCCAGG
    GAACCAGAAA GGAAAGAACA GCCCCCACAA AAGCACCTCA CCAGTAGCTG TCAGCCCCCA
    CCCCCTACCA AAGAGCTCCC TGGCCTGGCT TGTCAGCACT GCATTCTGCC CAGCCACCCT
    GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT GCTATAGACT CCAGCTTCCA GGATGCTCTC
    AGGCCCACTG CTGTGCGCAG TGACAGCAGC AGCCACAAGC CCAGGTCTGA CCCCGACCTG
    GGCCCTCCAA AGTCTAAGTT CCCAGACCGG CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG
    GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT
    AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT CCAGATCCCC AGAACACAGC CCGTGAAGTG
    ATTGGCCAGG GAGCCAGTGG GTACTCAGGC CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC
    ACAAAGCTGA GACCCACCAG CGCTCGGTGT CCCCCAGAGG CACTGGAGAT GCTTGGACAT
    CCACCAGGGC GAGGGCTCCT AGGGGCCAGT GAGTCAGTGG ACCAGAAACT CCCCACCTTC
    AGGGAAAGGC AAAACCTGTC TCCAAAGGCC CCCACACCAT CCACTGTGAA AGATTGTCCC
    CTTCCGTGCA AACAGACAGA CAGGAGCCCG AGTCCTCTGG CCACCAGCAC CAACGCTGGC
    AAGTCTGAAG GCAAGAAATG CACTGAAGCA CTTTACACCC CTGCGGATGG TGAGAAGCTG
    GGGGCCGGCT TGTCCTCGGT GCACAGCGAG GCTGGGCCTA AGGGCACAGA GAAGCCGGCA
    GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC AAGGGGAAAG GACTCAGTCC CCAGAAGCCA
    CTGACCGAGG CCAGCAAGCC CAGCGGCATG AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC
    TCTCTCCGCT GTGCCACCCT CCCAGAGAAG TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG
    GCCAGACCAG CAGTTCCAGA GACTTCTACC ACCTCCAGCG ACACCTCCTC TGCCAAGGCT
    GCTGGGCCTG CAGCAGAATC CCCAGCCTCC AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT
    GGTGGGGATG GCAGAACCCA AATGACCAAG AGCGACTCCT TGCCATGCTT CCGCAGCTCA
    GCCATCACTC TGGAGTCCCA GCACCCCAGC CCTGGCCTGG TGGTGGTCAC CAGCCACCGG
    GACAGGGCCC TCTCAGTAAC CGCCACCGTG GGAGAAACCA AAGGGAAAGA GCCAGCCCCC
    ACCCAGCCAG CTCAGACCAG GAACCAGAAC GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC
    GCTCCAAGCA CTGAGCGCCC CATCGTTCTT CCTTCTGAGA AGGACACTGG GCTGCGACAG
    AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837214.1
    CDS262..10092
    Translation

    Target ORF information:

    RefSeq Version XM_024981446.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC ACCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GAAGGAGCTG AGTCTTCCCA GAAGAGGAAG TCTGATAGAT
    TCCCAGAAGT GGAATTGCTT GGTCAAACGT TGCCGAACCA GCAACCGGAA AAGCTTGATA
    GGCAATGGAC CATCCCCAGC ATTGCCTCGG CCCCACTCAC CTCTTTCTGC TCATGCAGGA
    AATAGCCCTC AAGATAGTCC AAGAAATTTC TCCCCCAGTG CCTCTGCTCA TTTTTCATTT
    GCACGGAGGA CTGATGGACG CCGCTGGTCC TTGGCTTCTC TCCCCTCCTC TGGCTATGGG
    ACAAACACTC CCAGCTCTAC GGTCTCTTCA TCCTGTTCCT CCCAGGAGAA GTTGCATCAG
    TTACCGTATC AACCGACACC AGATGAATTA CACTTCTTAT CAAAACATTT TTGTACCACT
    GAAAGCATCA CCACAGAGAA CAGATGCAGA AACACACCCA TGCGCCCCCG TTCCCGAAGT
    TTGAGCCCTG GACGTTCTCC CGCCTGCTGT GACCATGAAA TAATTATGAT GAACCATGTC
    TACAAAGAAA GGTTCCCAAA GGCTACAGCT CAGATGGAAG AGCGCCTAAA GGAGATCATC
    ACCAGCTACT CTCCAGACCA CGTTCTCCCC CTTGCAGATG GAGTGCTCAG CTTCACTCAC
    CATCAGATTA TCGAACTGGC TCGCGATTGT TTGGATAAAT CCCACCAGGG TCTCATCACC
    TCACGATACT TCCTTGAATT ACAGCACAAA TTAGATAAGT TACTACAGGA GGCTCACAAT
    CGTTCAGAAA GTGGAGAGCT GGCATTTATT AAACAACTAG TTCGAAAGAT CCTGATTGTT
    ATTGCCCGCC CTGCTCGCTT ATTAGAGTGC CTGGAATTTG ATCCTGAAGA ATTTTACTAC
    CTATTGGAAG CAGCAGAAGG CCATGCCAAA GAAGGACAGG GTATTAAAAC TGACATTCCC
    AGGTACATCA TTAGCCAGCT TGGCCTCAAT AAAGATCCTC TGGAAGAAAT GGCTCAACTG
    GGAAACTATG ATAGTGGGAC AGCAGAAACA CCAGAAACAG ATGAATCAGT GAGTAGCTCT
    AATGCTTCCC TGAAACTTCG AAGGAAGCCT CGGGAAAGTG ATTTTGAAAC GATTAAATTG
    ATCAGCAATG GAGCCTACGG GGCAGTCTAT TTCGTTCGGC ATAAGGAATC CCGGCAGAGG
    TTTGCCATGA AGAAGATTAA CAAACAGAAC CTCATCCTCC GAAACCAGAT CCAGCAGGCC
    TTTGTGGAAC GGGATATCCT GACTTTTGCA GAAAACCCTT TTGTCGTCAG CATGTACTGC
    TCCTTTGAGA CCAGGCGCCA CTTGTGCATG GTCATGGAGT ATGTGGAAGG GGGTGACTGT
    GCCACGTTAA TGAAGAACAT GGGTCCTCTC CCCGTAGATA TGGCCAGGAT GTACTTTGCT
    GAGACGGTCC TGGCCTTGGA ATATCTACAT AATTATGGAA TTGTACATAG GGATTTGAAA
    CCAGACAATT TGCTGGTCAC CTCTATGGGG CACATAAAGC TGACCGATTT TGGATTATCC
    AAGGTGGGAC TCATGAGCAT GACTACCAAT CTTTATGAGG GTCATATTGA GAAGGATGCC
    CGAGAGTTCC TGGACAAGCA GGTCTGTGGC ACACCTGAAT ACATTGCACC AGAAGTGATT
    CTGAGGCAGG GCTACGGGAA GCCAGTGGAC TGGTGGGCCA TGGGGATTAT CCTCTATGAA
    TTTCTGGTTG GATGCGTGCC ATTCTTTGGG GACACTCCAG AAGAGCTATT TGGACAAGTC
    ATCAGTGATG AGATCAACTG GCCTGAAAAG GATGAGGCTC CACCCCCTGA TGCCCAGGAT
    CTGATTACCT TGCTCCTCAG GCAGAATCCC CTGGAGAGAC TGGGAACAGG TGGTGCATAT
    GAAGTCAAAC AGCATCGATT CTTCCGTTGC TTAGACTGGA ACAGTTTGCT GAGACAGAAG
    GCAGAATTCA TTCCCCAACT GGAATCTGAA GACGACACAA GTTATTTTGA TACTCGGTCA
    GAGAAGTACC ATCATATGGA GACTGAGGAA GAAGATGACA CGAACGATGA AGACTTTAAT
    GTGGAAATAA GGCAGTTTTC CTCATGTTCA CATAGGTTTT CTAAGGTCTT CAGCAGTATG
    GATCGAGGTA CTCAGAATTC AGGAGAAGAG AAGGAAGACC CTGGGGACAA AACTAAAAGC
    ACAGCTTTGC CGTCCACGGA AACACTAAGC TGGAGTTCAG AGTATTCTGA AATGCAACAG
    TTATCCACAT CCAACTCTTC AGACACTGAA AGCAACAGAC ATAGACGCGG TGCTGGGCTG
    CTTCCCAAGC TGGCTGTTTC AGCAGAGGCA GAACACGACG GGGCCGCTCC CCGCCCCAGA
    GAGCTGCACG AGGAGCCGGA AAAGCCAGCC CTGCCTGCTG CAGAGAATGC TCAGGAGGAG
    CCCGAGGTCA CTACCCCGGC CAGCACCATC AGCAGCTCCA CTCTGTCAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCGGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTATTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAACGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGTTCTCAG GTGGGTCCCA AGGATCCGGA AAGGAAAGAA
    CAGCCCCCAC AAAAGCACCT CACCAGTAAG TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG
    AAAGAACAGT GTTTGCAAAA GCACCTCACC AGTAGTTCTC AGCCGGGTCC CAAGGAACTG
    GAAAGGAAAG AACAACCTCC ACTAAAGCAT GTCACCAGTA GCTCTCAGCT GGGTCCCAGG
    GAACCAGAAA GGAAAGAACA GCCCCCACAA AAGCACCTCA CCAGTAGCTG TCAGCCCCCA
    CCCCCTACCA AAGAGCTCCC TGGCCTGGCT TGTCAGCACT GCATTCTGCC CAGCCACCCT
    GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT GCTATAGACT CCAGCTTCCA GGATGCTCTC
    AGGCCCACTG CTGTGCGCAG TGACAGCAGC AGCCACAAGC CCAGGTCTGA CCCCGACCTG
    GGCCCTCCAA AGTCTAAGTT CCCAGACCGG CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG
    GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT
    AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT CCAGATCCCC AGAACACAGC CCGTGAAGTG
    ATTGGCCAGG GAGCCAGTGG GTACTCAGGC CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC
    ACAAAGCTGA GACCCACCAG CGCTCGGTGT CCCCCAGAGG CACTGGAGAT GCTTGGACAT
    CCACCAGGGC GAGGGCTCCT AGGGGCCAGT GAGTCAGTGG ACCAGAAACT CCCCACCTTC
    AGGGAAAGGC AAAACCTGTC TCCAAAGGCC CCCACACCAT CCACTGTGAA AGATTGTCCC
    CTTCCGTGCA AACAGACAGA CAGGAGCCCG AGTCCTCTGG CCACCAGCAC CAACGCTGGC
    AAGTCTGAAG GCAAGAAATG CACTGAAGCA CTTTACACCC CTGCGGATGG TGAGAAGCTG
    GGGGCCGGCT TGTCCTCGGT GCACAGCGAG GCTGGGCCTA AGGGCACAGA GAAGCCGGCA
    GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC AAGGGGAAAG GACTCAGTCC CCAGAAGCCA
    CTGACCGAGG CCAGCAAGCC CAGCGGCATG AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC
    TCTCTCCGCT GTGCCACCCT CCCAGAGAAG TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG
    GCCAGACCAG CAGTTCCAGA GACTTCTACC ACCTCCAGCG ACACCTCCTC TGCCAAGGCT
    GCTGGGCCTG CAGCAGAATC CCCAGCCTCC AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT
    GGTGGGGATG GCAGAACCCA AATGACCAAG AGCGACTCCT TGCCATGCTT CCGCAGCTCA
    GCCATCACTC TGGAGTCCCA GCACCCCAGC CCTGGCCTGG TGGTGGTCAC CAGCCACCGG
    GACAGGGCCC TCTCAGTAAC CGCCACCGTG GGAGAAACCA AAGGGAAAGA GCCAGCCCCC
    ACCCAGCCAG CTCAGACCAG GAACCAGAAC GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC
    GCTCCAAGCA CTGAGCGCCC CATCGTTCTT CCTTCTGAGA AGGACACTGG GCTGCGACAG
    AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228113
    Clone ID Related Accession (Same CDS sequence) XM_024981447.1
    Accession Version XM_024981447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCAAGG 
    AGCCTGAGTC CAACCCCGTC CAGCCCAGGC AGTCCCTGTA GTCCTCTCTT CGCATTTCAC
    TTCTGGAGTT GCCGAACCAG CAACCGGAAA AGCTTGATAG GCAATGGACC ATCCCCAGCA
    TTGCCTCGGC CCCACTCACC TCTTTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA
    AGAAATTTCT CCCCCAGTGC CTCTGCTCAT TTTTCATTTG CACGGAGGAC TGATGGACGC
    CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT GGCTATGGGA CAAACACTCC CAGCTCTACG
    GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCGTATCA ACCGACACCA
    GATGAATTAC ACTTCTTATC AAAACATTTT TGTACCACTG AAAGCATCAC CACAGAGAAC
    AGATGCAGAA ACACACCCAT GCGCCCCCGT TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC
    GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG
    GCTACAGCTC AGATGGAAGA GCGCCTAAAG GAGATCATCA CCAGCTACTC TCCAGACCAC
    GTTCTCCCCC TTGCAGATGG AGTGCTCAGC TTCACTCACC ATCAGATTAT CGAACTGGCT
    CGCGATTGTT TGGATAAATC CCACCAGGGT CTCATCACCT CACGATACTT CCTTGAATTA
    CAGCACAAAT TAGATAAGTT ACTACAGGAG GCTCACAATC GTTCAGAAAG TGGAGAGCTG
    GCATTTATTA AACAACTAGT TCGAAAGATC CTGATTGTTA TTGCCCGCCC TGCTCGCTTA
    TTAGAGTGCC TGGAATTTGA TCCTGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC
    CATGCCAAAG AAGGACAGGG TATTAAAACT GACATTCCCA GGTACATCAT TAGCCAGCTT
    GGCCTCAATA AAGATCCTCT GGAAGAAATG GCTCAACTGG GAAACTATGA TAGTGGGACA
    GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCTTCCCT GAAACTTCGA
    AGGAAGCCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TCAGCAATGG AGCCTACGGG
    GCAGTCTATT TCGTTCGGCA TAAGGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAC
    AAACAGAACC TCATCCTCCG AAACCAGATC CAGCAGGCCT TTGTGGAACG GGATATCCTG
    ACTTTTGCAG AAAACCCTTT TGTCGTCAGC ATGTACTGCT CCTTTGAGAC CAGGCGCCAC
    TTGTGCATGG TCATGGAGTA TGTGGAAGGG GGTGACTGTG CCACGTTAAT GAAGAACATG
    GGTCCTCTCC CCGTAGATAT GGCCAGGATG TACTTTGCTG AGACGGTCCT GGCCTTGGAA
    TATCTACATA ATTATGGAAT TGTACATAGG GATTTGAAAC CAGACAATTT GCTGGTCACC
    TCTATGGGGC ACATAAAGCT GACCGATTTT GGATTATCCA AGGTGGGACT CATGAGCATG
    ACTACCAATC TTTATGAGGG TCATATTGAG AAGGATGCCC GAGAGTTCCT GGACAAGCAG
    GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG CTACGGGAAG
    CCAGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA
    TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG
    CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT GCCCAGGATC TGATTACCTT GCTCCTCAGG
    CAGAATCCCC TGGAGAGACT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC
    TTCCGTTGCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTCAT TCCCCAACTG
    GAATCTGAAG ACGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTACCA TCATATGGAG
    ACTGAGGAAG AAGATGACAC GAACGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCC
    TCATGTTCAC ATAGGTTTTC TAAGGTCTTC AGCAGTATGG ATCGAGGTAC TCAGAATTCA
    GGAGAAGAGA AGGAAGACCC TGGGGACAAA ACTAAAAGCA CAGCTTTGCC GTCCACGGAA
    ACACTAAGCT GGAGTTCAGA GTATTCTGAA ATGCAACAGT TATCCACATC CAACTCTTCA
    GACACTGAAA GCAACAGACA TAGACGCGGT GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA
    GCAGAGGCAG AACACGACGG GGCCGCTCCC CGCCCCAGAG AGCTGCACGA GGAGCCGGAA
    AAGCCAGCCC TGCCTGCTGC AGAGAATGCT CAGGAGGAGC CCGAGGTCAC TACCCCGGCC
    AGCACCATCA GCAGCTCCAC TCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA
    AATGGACGGA GCGAGTGTGT GGACAGTACA GACAATGCCT CCAAGCCCTC CAATGAGCCC
    GCCTCTCACA TGGCTCGGCA GCGCTTAGAA AGCACAGAGA AACAGAAAGC CTCGGGGAAA
    GTCACAAAGT CCCTTTCTGC CAGTGCCCTG TCCCTCATGA TCCCAGGAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCGGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTATTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAACGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGTTCTCAG GTGGGTCCCA AGGATCCGGA AAGGAAAGAA
    CAGCCCCCAC AAAAGCACCT CACCAGTAAG TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG
    AAAGAACAGT GTTTGCAAAA GCACCTCACC AGTAGTTCTC AGCCGGGTCC CAAGGAACTG
    GAAAGGAAAG AACAACCTCC ACTAAAGCAT GTCACCAGTA GCTCTCAGCT GGGTCCCAGG
    GAACCAGAAA GGAAAGAACA GCCCCCACAA AAGCACCTCA CCAGTAGCTG TCAGCCCCCA
    CCCCCTACCA AAGAGCTCCC TGGCCTGGCT TGTCAGCACT GCATTCTGCC CAGCCACCCT
    GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT GCTATAGACT CCAGCTTCCA GGATGCTCTC
    AGGCCCACTG CTGTGCGCAG TGACAGCAGC AGCCACAAGC CCAGGTCTGA CCCCGACCTG
    GGCCCTCCAA AGTCTAAGTT CCCAGACCGG CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG
    GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT
    AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT CCAGATCCCC AGAACACAGC CCGTGAAGTG
    ATTGGCCAGG GAGCCAGTGG GTACTCAGGC CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC
    ACAAAGCTGA GACCCACCAG CGCTCGGTGT CCCCCAGAGG CACTGGAGAT GCTTGGACAT
    CCACCAGGGC GAGGGCTCCT AGGGGCCAGT GAGTCAGTGG ACCAGAAACT CCCCACCTTC
    AGGGAAAGGC AAAACCTGTC TCCAAAGGCC CCCACACCAT CCACTGTGAA AGATTGTCCC
    CTTCCGTGCA AACAGACAGA CAGGAGCCCG AGTCCTCTGG CCACCAGCAC CAACGCTGGC
    AAGTCTGAAG GCAAGAAATG CACTGAAGCA CTTTACACCC CTGCGGATGG TGAGAAGCTG
    GGGGCCGGCT TGTCCTCGGT GCACAGCGAG GCTGGGCCTA AGGGCACAGA GAAGCCGGCA
    GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC AAGGGGAAAG GACTCAGTCC CCAGAAGCCA
    CTGACCGAGG CCAGCAAGCC CAGCGGCATG AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC
    TCTCTCCGCT GTGCCACCCT CCCAGAGAAG TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG
    GCCAGACCAG CAGTTCCAGA GACTTCTACC ACCTCCAGCG ACACCTCCTC TGCCAAGGCT
    GCTGGGCCTG CAGCAGAATC CCCAGCCTCC AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT
    GGTGGGGATG GCAGAACCCA AATGACCAAG AGCGACTCCT TGCCATGCTT CCGCAGCTCA
    GCCATCACTC TGGAGTCCCA GCACCCCAGC CCTGGCCTGG TGGTGGTCAC CAGCCACCGG
    GACAGGGCCC TCTCAGTAAC CGCCACCGTG GGAGAAACCA AAGGGAAAGA GCCAGCCCCC
    ACCCAGCCAG CTCAGACCAG GAACCAGAAC GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC
    GCTCCAAGCA CTGAGCGCCC CATCGTTCTT CCTTCTGAGA AGGACACTGG GCTGCGACAG
    AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837215.1
    CDS205..9675
    Translation

    Target ORF information:

    RefSeq Version XM_024981447.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGAAAGCCC AGCGGGAAAG ACTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCAAGG 
    AGCCTGAGTC CAACCCCGTC CAGCCCAGGC AGTCCCTGTA GTCCTCTCTT CGCATTTCAC
    TTCTGGAGTT GCCGAACCAG CAACCGGAAA AGCTTGATAG GCAATGGACC ATCCCCAGCA
    TTGCCTCGGC CCCACTCACC TCTTTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA
    AGAAATTTCT CCCCCAGTGC CTCTGCTCAT TTTTCATTTG CACGGAGGAC TGATGGACGC
    CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT GGCTATGGGA CAAACACTCC CAGCTCTACG
    GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCGTATCA ACCGACACCA
    GATGAATTAC ACTTCTTATC AAAACATTTT TGTACCACTG AAAGCATCAC CACAGAGAAC
    AGATGCAGAA ACACACCCAT GCGCCCCCGT TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC
    GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG
    GCTACAGCTC AGATGGAAGA GCGCCTAAAG GAGATCATCA CCAGCTACTC TCCAGACCAC
    GTTCTCCCCC TTGCAGATGG AGTGCTCAGC TTCACTCACC ATCAGATTAT CGAACTGGCT
    CGCGATTGTT TGGATAAATC CCACCAGGGT CTCATCACCT CACGATACTT CCTTGAATTA
    CAGCACAAAT TAGATAAGTT ACTACAGGAG GCTCACAATC GTTCAGAAAG TGGAGAGCTG
    GCATTTATTA AACAACTAGT TCGAAAGATC CTGATTGTTA TTGCCCGCCC TGCTCGCTTA
    TTAGAGTGCC TGGAATTTGA TCCTGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC
    CATGCCAAAG AAGGACAGGG TATTAAAACT GACATTCCCA GGTACATCAT TAGCCAGCTT
    GGCCTCAATA AAGATCCTCT GGAAGAAATG GCTCAACTGG GAAACTATGA TAGTGGGACA
    GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCTTCCCT GAAACTTCGA
    AGGAAGCCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TCAGCAATGG AGCCTACGGG
    GCAGTCTATT TCGTTCGGCA TAAGGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAC
    AAACAGAACC TCATCCTCCG AAACCAGATC CAGCAGGCCT TTGTGGAACG GGATATCCTG
    ACTTTTGCAG AAAACCCTTT TGTCGTCAGC ATGTACTGCT CCTTTGAGAC CAGGCGCCAC
    TTGTGCATGG TCATGGAGTA TGTGGAAGGG GGTGACTGTG CCACGTTAAT GAAGAACATG
    GGTCCTCTCC CCGTAGATAT GGCCAGGATG TACTTTGCTG AGACGGTCCT GGCCTTGGAA
    TATCTACATA ATTATGGAAT TGTACATAGG GATTTGAAAC CAGACAATTT GCTGGTCACC
    TCTATGGGGC ACATAAAGCT GACCGATTTT GGATTATCCA AGGTGGGACT CATGAGCATG
    ACTACCAATC TTTATGAGGG TCATATTGAG AAGGATGCCC GAGAGTTCCT GGACAAGCAG
    GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG CTACGGGAAG
    CCAGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA
    TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG
    CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT GCCCAGGATC TGATTACCTT GCTCCTCAGG
    CAGAATCCCC TGGAGAGACT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC
    TTCCGTTGCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTCAT TCCCCAACTG
    GAATCTGAAG ACGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTACCA TCATATGGAG
    ACTGAGGAAG AAGATGACAC GAACGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCC
    TCATGTTCAC ATAGGTTTTC TAAGGTCTTC AGCAGTATGG ATCGAGGTAC TCAGAATTCA
    GGAGAAGAGA AGGAAGACCC TGGGGACAAA ACTAAAAGCA CAGCTTTGCC GTCCACGGAA
    ACACTAAGCT GGAGTTCAGA GTATTCTGAA ATGCAACAGT TATCCACATC CAACTCTTCA
    GACACTGAAA GCAACAGACA TAGACGCGGT GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA
    GCAGAGGCAG AACACGACGG GGCCGCTCCC CGCCCCAGAG AGCTGCACGA GGAGCCGGAA
    AAGCCAGCCC TGCCTGCTGC AGAGAATGCT CAGGAGGAGC CCGAGGTCAC TACCCCGGCC
    AGCACCATCA GCAGCTCCAC TCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA
    AATGGACGGA GCGAGTGTGT GGACAGTACA GACAATGCCT CCAAGCCCTC CAATGAGCCC
    GCCTCTCACA TGGCTCGGCA GCGCTTAGAA AGCACAGAGA AACAGAAAGC CTCGGGGAAA
    GTCACAAAGT CCCTTTCTGC CAGTGCCCTG TCCCTCATGA TCCCAGGAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCGGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTATTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAACGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGTTCTCAG GTGGGTCCCA AGGATCCGGA AAGGAAAGAA
    CAGCCCCCAC AAAAGCACCT CACCAGTAAG TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG
    AAAGAACAGT GTTTGCAAAA GCACCTCACC AGTAGTTCTC AGCCGGGTCC CAAGGAACTG
    GAAAGGAAAG AACAACCTCC ACTAAAGCAT GTCACCAGTA GCTCTCAGCT GGGTCCCAGG
    GAACCAGAAA GGAAAGAACA GCCCCCACAA AAGCACCTCA CCAGTAGCTG TCAGCCCCCA
    CCCCCTACCA AAGAGCTCCC TGGCCTGGCT TGTCAGCACT GCATTCTGCC CAGCCACCCT
    GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT GCTATAGACT CCAGCTTCCA GGATGCTCTC
    AGGCCCACTG CTGTGCGCAG TGACAGCAGC AGCCACAAGC CCAGGTCTGA CCCCGACCTG
    GGCCCTCCAA AGTCTAAGTT CCCAGACCGG CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG
    GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT
    AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT CCAGATCCCC AGAACACAGC CCGTGAAGTG
    ATTGGCCAGG GAGCCAGTGG GTACTCAGGC CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC
    ACAAAGCTGA GACCCACCAG CGCTCGGTGT CCCCCAGAGG CACTGGAGAT GCTTGGACAT
    CCACCAGGGC GAGGGCTCCT AGGGGCCAGT GAGTCAGTGG ACCAGAAACT CCCCACCTTC
    AGGGAAAGGC AAAACCTGTC TCCAAAGGCC CCCACACCAT CCACTGTGAA AGATTGTCCC
    CTTCCGTGCA AACAGACAGA CAGGAGCCCG AGTCCTCTGG CCACCAGCAC CAACGCTGGC
    AAGTCTGAAG GCAAGAAATG CACTGAAGCA CTTTACACCC CTGCGGATGG TGAGAAGCTG
    GGGGCCGGCT TGTCCTCGGT GCACAGCGAG GCTGGGCCTA AGGGCACAGA GAAGCCGGCA
    GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC AAGGGGAAAG GACTCAGTCC CCAGAAGCCA
    CTGACCGAGG CCAGCAAGCC CAGCGGCATG AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC
    TCTCTCCGCT GTGCCACCCT CCCAGAGAAG TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG
    GCCAGACCAG CAGTTCCAGA GACTTCTACC ACCTCCAGCG ACACCTCCTC TGCCAAGGCT
    GCTGGGCCTG CAGCAGAATC CCCAGCCTCC AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT
    GGTGGGGATG GCAGAACCCA AATGACCAAG AGCGACTCCT TGCCATGCTT CCGCAGCTCA
    GCCATCACTC TGGAGTCCCA GCACCCCAGC CCTGGCCTGG TGGTGGTCAC CAGCCACCGG
    GACAGGGCCC TCTCAGTAAC CGCCACCGTG GGAGAAACCA AAGGGAAAGA GCCAGCCCCC
    ACCCAGCCAG CTCAGACCAG GAACCAGAAC GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC
    GCTCCAAGCA CTGAGCGCCC CATCGTTCTT CCTTCTGAGA AGGACACTGG GCTGCGACAG
    AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228114
    Clone ID Related Accession (Same CDS sequence) XM_024981448.1
    Accession Version XM_024981448.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9421
    ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTTCCCAGA 
    AGAGGAAGTC TGATAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACCAGC
    AACCGGAAAA GCTTGATAGG CAATGGACCA TCCCCAGCAT TGCCTCGGCC CCACTCACCT
    CTTTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC
    TCTGCTCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCCTT GGCTTCTCTC
    CCCTCCTCTG GCTATGGGAC AAACACTCCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC
    CAGGAGAAGT TGCATCAGTT ACCGTATCAA CCGACACCAG ATGAATTACA CTTCTTATCA
    AAACATTTTT GTACCACTGA AAGCATCACC ACAGAGAACA GATGCAGAAA CACACCCATG
    CGCCCCCGTT CCCGAAGTTT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA
    ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAG
    CGCCTAAAGG AGATCATCAC CAGCTACTCT CCAGACCACG TTCTCCCCCT TGCAGATGGA
    GTGCTCAGCT TCACTCACCA TCAGATTATC GAACTGGCTC GCGATTGTTT GGATAAATCC
    CACCAGGGTC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTA
    CTACAGGAGG CTCACAATCG TTCAGAAAGT GGAGAGCTGG CATTTATTAA ACAACTAGTT
    CGAAAGATCC TGATTGTTAT TGCCCGCCCT GCTCGCTTAT TAGAGTGCCT GGAATTTGAT
    CCTGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT
    ATTAAAACTG ACATTCCCAG GTACATCATT AGCCAGCTTG GCCTCAATAA AGATCCTCTG
    GAAGAAATGG CTCAACTGGG AAACTATGAT AGTGGGACAG CAGAAACACC AGAAACAGAT
    GAATCAGTGA GTAGCTCTAA TGCTTCCCTG AAACTTCGAA GGAAGCCTCG GGAAAGTGAT
    TTTGAAACGA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTATTT CGTTCGGCAT
    AAGGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAACA AACAGAACCT CATCCTCCGA
    AACCAGATCC AGCAGGCCTT TGTGGAACGG GATATCCTGA CTTTTGCAGA AAACCCTTTT
    GTCGTCAGCA TGTACTGCTC CTTTGAGACC AGGCGCCACT TGTGCATGGT CATGGAGTAT
    GTGGAAGGGG GTGACTGTGC CACGTTAATG AAGAACATGG GTCCTCTCCC CGTAGATATG
    GCCAGGATGT ACTTTGCTGA GACGGTCCTG GCCTTGGAAT ATCTACATAA TTATGGAATT
    GTACATAGGG ATTTGAAACC AGACAATTTG CTGGTCACCT CTATGGGGCA CATAAAGCTG
    ACCGATTTTG GATTATCCAA GGTGGGACTC ATGAGCATGA CTACCAATCT TTATGAGGGT
    CATATTGAGA AGGATGCCCG AGAGTTCCTG GACAAGCAGG TCTGTGGCAC ACCTGAATAC
    ATTGCACCAG AAGTGATTCT GAGGCAGGGC TACGGGAAGC CAGTGGACTG GTGGGCCATG
    GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA CACTCCAGAA
    GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAAAAGGA TGAGGCTCCA
    CCCCCTGATG CCCAGGATCT GATTACCTTG CTCCTCAGGC AGAATCCCCT GGAGAGACTG
    GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTGCTT AGACTGGAAC
    AGTTTGCTGA GACAGAAGGC AGAATTCATT CCCCAACTGG AATCTGAAGA CGACACAAGT
    TATTTTGATA CTCGGTCAGA GAAGTACCAT CATATGGAGA CTGAGGAAGA AGATGACACG
    AACGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCCT CATGTTCACA TAGGTTTTCT
    AAGGTCTTCA GCAGTATGGA TCGAGGTACT CAGAATTCAG GAGAAGAGAA GGAAGACCCT
    GGGGACAAAA CTAAAAGCAC AGCTTTGCCG TCCACGGAAA CACTAAGCTG GAGTTCAGAG
    TATTCTGAAA TGCAACAGTT ATCCACATCC AACTCTTCAG ACACTGAAAG CAACAGACAT
    AGACGCGGTG CTGGGCTGCT TCCCAAGCTG GCTGTTTCAG CAGAGGCAGA ACACGACGGG
    GCCGCTCCCC GCCCCAGAGA GCTGCACGAG GAGCCGGAAA AGCCAGCCCT GCCTGCTGCA
    GAGAATGCTC AGGAGGAGCC CGAGGTCACT ACCCCGGCCA GCACCATCAG CAGCTCCACT
    CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGGAG CGAGTGTGTG
    GACAGTACAG ACAATGCCTC CAAGCCCTCC AATGAGCCCG CCTCTCACAT GGCTCGGCAG
    CGCTTAGAAA GCACAGAGAA ACAGAAAGCC TCGGGGAAAG TCACAAAGTC CCTTTCTGCC
    AGTGCCCTGT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TCTCTCCGCT GGGAAGTCCG
    ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCCTCAA GGGATTCCTC TCCCAGCCGA
    GATTCTTCAG GAGCTTCTGC CAGTCCACAC CAGCCCATTG TGATCCACAG TTTGGGGAAG
    AACTATGGTT TCACCATTCG AGCCATCCGG GTGTACGTGG GAGACAGCGA CATTTACACA
    GTGCATCATA TCGTTTGGAA TGTAGAAGAA GGAAGTCCAG CTTGCCAGGC AGGACTGAAA
    GCTGGGGATC TGATCACACA CATCAATGGA GAACCGGTGC ACGGACTTGT CCACACAGAA
    GTTATAGAGC TCCTGCTAAA GAGTGGAAAT AAGGTGTCAA TCACGACCAC CCCATTTGAA
    AACACATCAA TCAAAACGGG ACCGGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG
    CGGAGCAAGA AATCTAAGAA GAAGGAAAGT CTGGAAAGGA GGAGATCCCT TTTCAAAAAA
    CTAGCTAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACCGA
    TCCCTGTCGT CAGGGGAGAG CCTCCCAGGG TCCCCCACTC ACAGCTTGTC TCCCCGATCC
    CCTACACCCA GCTACCGTTC CACCCCCGAC TTCCCATCCG GCACTAACTC CTCCCAGAGC
    AGCTCCCCAA GCTCCAGCGC TCCCAACTCC CCAGCAGGGT CAGGACACGT CCGGCCCAGC
    ACGCTCCATG GTCTGGCGCC CAAACTCAGC GGGCAGCGCT ACCGGTCAGG AAGGCGGAAG
    TCAGCTGGCA GCATCCCCCT GTCTCCGCTG GCCCGGACAC CCTCTCCGAC CCCACAGCCC
    ACCTCCCCGC AGCGGTCCCC ATCCCCTCTG CTGGGGCACT CACTTGGCAA TTCCAAGATG
    GCTCAAGCCT TTCCCAGCAA AATGCACTCC CCACCCACCA TCGTCAGGCA CGTCGTGAGG
    CCCAAGAGTG CAGAACCCCC TCGGTCCCCG CTGCTCAAGC GCGTGCAGTC GGAGGAGAAG
    CTGTCTCCCT CCTATGGAGG CGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTTGAG
    GTCACCCAGG AGGAGGTGCC GCGGGAGCAG CAACAGCGGG AGGTGACCTT GCAGAGCCTG
    GAGGAGAACG TGTGCGACGC GCCTGCCCTC AGCCGGGCCA GGCCCGTGGA GCAGGGCTGC
    CTGAAACGCC CCGTGTCCCG GAAGCTGGGC CGCCAGGAGT CTGTGGACGA GCTGGACCGA
    GAGAAGCTAA AGGCCAAGGT GGTGGTGAAG AAACCTGACG GGCTCTCGGA AAAACAGGAA
    TCCCGCCCGA AGCCCCACGG ACTCGGCAGT GACTTGGAAA ACCTCTGTGC TTTTAGGCTC
    GAAGAGAGAG ACAAGAAAAT CTATCCAAAA GCTTCAGAGA GGTCAAATCA TTTTGAAAGC
    AAAGCAGCCG TGCAGGAGGC CCAGCCCCTG TGCAGCCTTT TGAAAGATGC TCTCCACAAG
    CAGGCCAATG TGCGGGCCAG CGAGGGTGTC GCCTCTGAGG GGGCAACGGC AGCTGGGGAC
    CACGGCCAGG GCACCTGTGA CTTCAAACGA GCCTCAACGC ACAGCACCCT CCAAGACGGC
    CTCTGTCACA CCACCCACAG GTCCACCTCC GGGAAGGGGG ACACCGCAGA GAAGGCCCCC
    CAGGCCAAGG AGGGTCTGCG GTGTGAGAAG TTAGACGGCA AGTTGGCCAA CATCGATTAC
    CTCCGAAAGA AGATGTCCCT TGAAGACAAA GACGAGAGCC TCTGTTCTGT GCTCAAGCCC
    AAGGTGACAG CTGGTGCCCA GGACTGCCTG CCTGGGAGCC TGGGCCGGCC CGTGGGAGGG
    CAGCAGGAGG CCCCGCCAGC CTCCGAGGCC CGCACACCGT TCATCGGCAG CGCCCACGCG
    GCTCAGCTCA GCGCCGTCTC CTTTGTCCCT CTCAAGGCCC TTTCTGGCCG GGTGGACAGT
    GGAGCGGAGA AGCCGGGCTT GGCTGCCCCA GAGTCGCCTG TCCGGAAAAG CCCCTCCGAG
    TATAAGCTGG AAGGGCGGTC TGTGTCGTGC CTGAAGCCCA TTGAAGGCAC CTTGGACATC
    GCTCTCCTGT CGGGACCTCA GGCCTCCAAG ACAGAGCTGC CTTCCCCGGA GCCTGCCCAG
    AGCCCCAGCC CAGGCAGTGA TGTGGAGTCC CCCATGCCCC TCGCTCTCCC CAGCAGCACA
    GGGAGGAAGG CTGACACTGC GGGTCAGAGA GAGTCCTCCC CTGCCGGCTT CAAGGTGAAC
    AAATCCTACC TGCTTGAGCC CCGGTTCCCA CCGGCCAGCC GGGGTCTGCA GAGTTCACCG
    GTGGTTGCCC CGCCTGACCC AGAGCTGAAG CTGGACAAGA AGGTCTCCCA GGCAGCCCGT
    GCCCCAGCGA CCATCATGGA GAGCCATCCC CAGCGGGGAG AGGGCCACCC CAGCCAACAC
    CAAGACCACA GCCCCGACGT TAAGCTCCTT CCCTTCCCAG GGCAGAACCT CCAAGGTGCC
    AACACGTCTA GATCCCGCAG CCCACTCTTG CCTGAAAGTG TCCCCCCTAG AGAGAAGCCG
    AGTGGGAGAG AGTTGTCCGA AAGGGGCCCT TCCATAGTCG GGAGCGAGTG GTCAGCTGTG
    AGGCCCGACA TCCGCAGAGA CCCCTCCAAG GAGCTGTGTC CCCCAGAAGC TGCTAAACCC
    AGCGACAACT CCCAAACCCC CCCTGTGGTG GGGAGGACCC GCCTGGATGT CTCCACCCAG
    ACCCAGGCCT TGGAGAAAGC ATGCGGGCCT TGCGGGAGAG CAAAGCCCAG AGACGGCCGA
    GAGGAGGTGA GGCTGCCGGC CAGAGAGGAC TCCTCTTTGC GCTCTGCGGG GGCTCCTTGT
    GAGAGAGAGC TGGGCGCAGA GAGAGGCCTG GAATCCAAGA TGGAACCTGC TTCTACCAGG
    TGGTCTCTGG AGCCGCCCAG GCCAGAGAGT GAGAAGAGTG GAAGGCCTTC TGGTTTCCAG
    TCTGTGCAGA AAGACGGTCC CAAGGAACCG GAGAGGAAAG AACAACCTCT GCAAAAGTAC
    CTCACCAGTT CTCAGCCGGG TCCCAAGGAA CCCGAGAGGA AAGAACAGCC CCTGCAAAAG
    CACCTCCCCA GTAGTTCTCA GCCGGGTCCC AAGGAACCCG AGAGGAAAGA ACAGCCCCTG
    CAAAAGCACG TCACCAGTAG TTCTCAGCCG GGTCCCAAGG AACCGGAGAG GAAAGAACAG
    CCTCTGCAAA AGCACCTCCC CAGTAGTTCT CAGCCGGGTC CCAAGGAACC GGAGAGGAAA
    GAACAGCCCC TGCAAAAGCA CGTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCCGAG
    AGGAAAGAAC AGCCTCTGCA AAAGCACCTC CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CTGGAAAGGA AAGAACAGTG TTTGCAGAAG CACCTCACCA GTAGCTCTCA GCTGGGTCCC
    AAGGAACCAG AAATGAAAGA ACAGCCCCCA CAAAAGCACG TCACCAGTAG TTCTCAACTG
    TGTCCCAAGG AACTGGAAAG GAAAGAACAA CCTCCACAAA AGCACCTCAC TATTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAGAGCA CGTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAACCGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACTTC
    ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA CCAGAAACGA AAGAACAGCC TCTGCAAAAG
    CACCTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCGG AAAGGAAAGA ACAGCTTCTG
    CAAGAGCACC TCACCAGTAG CTCTCAGCTG AGTCCCAAGG AACCGGAAAG GAAAGAACAG
    TGTTTGCAGA AGCACCTCAC CAGTAGTTCT CAGCTGGGTC CCAAGGAACC AGAAATGAAA
    GAACAGCCTC TGCAAAAGCA CCTCACCAGT AGCTCTCAGC TGAGTCCCAA GGAACCAGAA
    AGGAAAGAAC AGCCTCTGCA AAAGCACGTC ACCAGTAGCT CTCAGCTGAG CCCCAAGGAG
    CCGGAAAGGA AAGAACAGCC TCTGCAAAAG CACCTCACCA GTAGCTGTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACG TCACCAGTAG CTCTCAGCTG
    AGCCCCAAGG AGCCAGAAAG GAAAGAACAG CCTCTGCAAA AGCACCTCAC CATTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAGCCAGAA AGGAAAGAAC AGCTTCTGCA AAAGCACCTC
    ACCATTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCT TCTGCAAAAG
    CACCTCACCA TTAGCTCTCA GCTGAGTCCC AAGGAGCCGG AAAAGAAAGA ACAGCCTCTG
    CAAAAGCACG TCACCAGTAG CTCTCAGCTG AGTCCCAAGG AGCCGGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CATTAGCTCT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CGTCACCAGT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCTTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAGGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACG TCACCAGTAG CTCTCAGCTG
    AGTCCCAAGG AACCGGAAAG GAAAGAACAG TATTTGCAGA AGCACCTCAC CAGTAGTTCT
    CAGCTGGGTC CCAAGGAACC AGAAACGAAA GAACAGCCTC TGCAAAAGCA CCTCACCAGT
    AGTTCTCAGG TGGGTCCCAA GGATCCGGAA AGGAAAGAAC AGCCCCCACA AAAGCACCTC
    ACCAGTAAGT CTCAGCCGGG TCCCAAGGAA CCGGAAAGGA AAGAACAGTG TTTGCAAAAG
    CACCTCACCA GTAGTTCTCA GCCGGGTCCC AAGGAACTGG AAAGGAAAGA ACAACCTCCA
    CTAAAGCATG TCACCAGTAG CTCTCAGCTG GGTCCCAGGG AACCAGAAAG GAAAGAACAG
    CCCCCACAAA AGCACCTCAC CAGTAGCTGT CAGCCCCCAC CCCCTACCAA AGAGCTCCCT
    GGCCTGGCTT GTCAGCACTG CATTCTGCCC AGCCACCCTG CTGCCTCTCT GCTGGGGAGC
    AAGCCCTGTG CTATAGACTC CAGCTTCCAG GATGCTCTCA GGCCCACTGC TGTGCGCAGT
    GACAGCAGCA GCCACAAGCC CAGGTCTGAC CCCGACCTGG GCCCTCCAAA GTCTAAGTTC
    CCAGACCGGC CCCTCTCCTC CCAGAAGCCG AGCGCTGAGG CTGCAGTGAG CAAAGAGCCT
    GGCGCTCCGA CCCTCGGCAG AGCTGGGGTG GGGAGTGGTA AGGGTGTGTC AGAGGAGTTC
    CCTGTCCTTC CAGATCCCCA GAACACAGCC CGTGAAGTGA TTGGCCAGGG AGCCAGTGGG
    TACTCAGGCC CCCTGCCCAT GGAAGGGGGT CCTCTAGACA CAAAGCTGAG ACCCACCAGC
    GCTCGGTGTC CCCCAGAGGC ACTGGAGATG CTTGGACATC CACCAGGGCG AGGGCTCCTA
    GGGGCCAGTG AGTCAGTGGA CCAGAAACTC CCCACCTTCA GGGAAAGGCA AAACCTGTCT
    CCAAAGGCCC CCACACCATC CACTGTGAAA GATTGTCCCC TTCCGTGCAA ACAGACAGAC
    AGGAGCCCGA GTCCTCTGGC CACCAGCACC AACGCTGGCA AGTCTGAAGG CAAGAAATGC
    ACTGAAGCAC TTTACACCCC TGCGGATGGT GAGAAGCTGG GGGCCGGCTT GTCCTCGGTG
    CACAGCGAGG CTGGGCCTAA GGGCACAGAG AAGCCGGCAG CAGCCGCTGG GAAGGGCTGG
    CCAGAGGCCA AGGGGAAAGG ACTCAGTCCC CAGAAGCCAC TGACCGAGGC CAGCAAGCCC
    AGCGGCATGA AGCGGTCACC TTCAGCCACG GTGCAGAGCT CTCTCCGCTG TGCCACCCTC
    CCAGAGAAGT CTCTGAGCTA CTCCTGTGGC TTTCCCGAGG CCAGACCAGC AGTTCCAGAG
    ACTTCTACCA CCTCCAGCGA CACCTCCTCT GCCAAGGCTG CTGGGCCTGC AGCAGAATCC
    CCAGCCTCCA GCAGCAGGGA CCAGCAGAAA CCCCTGCCTG GTGGGGATGG CAGAACCCAA
    ATGACCAAGA GCGACTCCTT GCCATGCTTC CGCAGCTCAG CCATCACTCT GGAGTCCCAG
    CACCCCAGCC CTGGCCTGGT GGTGGTCACC AGCCACCGGG ACAGGGCCCT CTCAGTAACC
    GCCACCGTGG GAGAAACCAA AGGGAAAGAG CCAGCCCCCA CCCAGCCAGC TCAGACCAGG
    AACCAGAACG TGAGCAGAGA GGCGGCCAAG CCGTCCCCCG CTCCAAGCAC TGAGCGCCCC
    ATCGTTCTTC CTTCTGAGAA GGACACTGGG CTGCGACAGA GGCGGGGGAA AGAGAGTTTG
    CGCGGCAGCC CCCACAAAAA GGCCTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837216.1
    CDS64..9513
    Translation

    Target ORF information:

    RefSeq Version XM_024981448.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTTCCCAGA 
    AGAGGAAGTC TGATAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACCAGC
    AACCGGAAAA GCTTGATAGG CAATGGACCA TCCCCAGCAT TGCCTCGGCC CCACTCACCT
    CTTTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC
    TCTGCTCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCCTT GGCTTCTCTC
    CCCTCCTCTG GCTATGGGAC AAACACTCCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC
    CAGGAGAAGT TGCATCAGTT ACCGTATCAA CCGACACCAG ATGAATTACA CTTCTTATCA
    AAACATTTTT GTACCACTGA AAGCATCACC ACAGAGAACA GATGCAGAAA CACACCCATG
    CGCCCCCGTT CCCGAAGTTT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA
    ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAG
    CGCCTAAAGG AGATCATCAC CAGCTACTCT CCAGACCACG TTCTCCCCCT TGCAGATGGA
    GTGCTCAGCT TCACTCACCA TCAGATTATC GAACTGGCTC GCGATTGTTT GGATAAATCC
    CACCAGGGTC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTA
    CTACAGGAGG CTCACAATCG TTCAGAAAGT GGAGAGCTGG CATTTATTAA ACAACTAGTT
    CGAAAGATCC TGATTGTTAT TGCCCGCCCT GCTCGCTTAT TAGAGTGCCT GGAATTTGAT
    CCTGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT
    ATTAAAACTG ACATTCCCAG GTACATCATT AGCCAGCTTG GCCTCAATAA AGATCCTCTG
    GAAGAAATGG CTCAACTGGG AAACTATGAT AGTGGGACAG CAGAAACACC AGAAACAGAT
    GAATCAGTGA GTAGCTCTAA TGCTTCCCTG AAACTTCGAA GGAAGCCTCG GGAAAGTGAT
    TTTGAAACGA TTAAATTGAT CAGCAATGGA GCCTACGGGG CAGTCTATTT CGTTCGGCAT
    AAGGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAACA AACAGAACCT CATCCTCCGA
    AACCAGATCC AGCAGGCCTT TGTGGAACGG GATATCCTGA CTTTTGCAGA AAACCCTTTT
    GTCGTCAGCA TGTACTGCTC CTTTGAGACC AGGCGCCACT TGTGCATGGT CATGGAGTAT
    GTGGAAGGGG GTGACTGTGC CACGTTAATG AAGAACATGG GTCCTCTCCC CGTAGATATG
    GCCAGGATGT ACTTTGCTGA GACGGTCCTG GCCTTGGAAT ATCTACATAA TTATGGAATT
    GTACATAGGG ATTTGAAACC AGACAATTTG CTGGTCACCT CTATGGGGCA CATAAAGCTG
    ACCGATTTTG GATTATCCAA GGTGGGACTC ATGAGCATGA CTACCAATCT TTATGAGGGT
    CATATTGAGA AGGATGCCCG AGAGTTCCTG GACAAGCAGG TCTGTGGCAC ACCTGAATAC
    ATTGCACCAG AAGTGATTCT GAGGCAGGGC TACGGGAAGC CAGTGGACTG GTGGGCCATG
    GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA CACTCCAGAA
    GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAAAAGGA TGAGGCTCCA
    CCCCCTGATG CCCAGGATCT GATTACCTTG CTCCTCAGGC AGAATCCCCT GGAGAGACTG
    GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTGCTT AGACTGGAAC
    AGTTTGCTGA GACAGAAGGC AGAATTCATT CCCCAACTGG AATCTGAAGA CGACACAAGT
    TATTTTGATA CTCGGTCAGA GAAGTACCAT CATATGGAGA CTGAGGAAGA AGATGACACG
    AACGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCCT CATGTTCACA TAGGTTTTCT
    AAGGTCTTCA GCAGTATGGA TCGAGGTACT CAGAATTCAG GAGAAGAGAA GGAAGACCCT
    GGGGACAAAA CTAAAAGCAC AGCTTTGCCG TCCACGGAAA CACTAAGCTG GAGTTCAGAG
    TATTCTGAAA TGCAACAGTT ATCCACATCC AACTCTTCAG ACACTGAAAG CAACAGACAT
    AGACGCGGTG CTGGGCTGCT TCCCAAGCTG GCTGTTTCAG CAGAGGCAGA ACACGACGGG
    GCCGCTCCCC GCCCCAGAGA GCTGCACGAG GAGCCGGAAA AGCCAGCCCT GCCTGCTGCA
    GAGAATGCTC AGGAGGAGCC CGAGGTCACT ACCCCGGCCA GCACCATCAG CAGCTCCACT
    CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGGAG CGAGTGTGTG
    GACAGTACAG ACAATGCCTC CAAGCCCTCC AATGAGCCCG CCTCTCACAT GGCTCGGCAG
    CGCTTAGAAA GCACAGAGAA ACAGAAAGCC TCGGGGAAAG TCACAAAGTC CCTTTCTGCC
    AGTGCCCTGT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TCTCTCCGCT GGGAAGTCCG
    ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCCTCAA GGGATTCCTC TCCCAGCCGA
    GATTCTTCAG GAGCTTCTGC CAGTCCACAC CAGCCCATTG TGATCCACAG TTTGGGGAAG
    AACTATGGTT TCACCATTCG AGCCATCCGG GTGTACGTGG GAGACAGCGA CATTTACACA
    GTGCATCATA TCGTTTGGAA TGTAGAAGAA GGAAGTCCAG CTTGCCAGGC AGGACTGAAA
    GCTGGGGATC TGATCACACA CATCAATGGA GAACCGGTGC ACGGACTTGT CCACACAGAA
    GTTATAGAGC TCCTGCTAAA GAGTGGAAAT AAGGTGTCAA TCACGACCAC CCCATTTGAA
    AACACATCAA TCAAAACGGG ACCGGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG
    CGGAGCAAGA AATCTAAGAA GAAGGAAAGT CTGGAAAGGA GGAGATCCCT TTTCAAAAAA
    CTAGCTAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACCGA
    TCCCTGTCGT CAGGGGAGAG CCTCCCAGGG TCCCCCACTC ACAGCTTGTC TCCCCGATCC
    CCTACACCCA GCTACCGTTC CACCCCCGAC TTCCCATCCG GCACTAACTC CTCCCAGAGC
    AGCTCCCCAA GCTCCAGCGC TCCCAACTCC CCAGCAGGGT CAGGACACGT CCGGCCCAGC
    ACGCTCCATG GTCTGGCGCC CAAACTCAGC GGGCAGCGCT ACCGGTCAGG AAGGCGGAAG
    TCAGCTGGCA GCATCCCCCT GTCTCCGCTG GCCCGGACAC CCTCTCCGAC CCCACAGCCC
    ACCTCCCCGC AGCGGTCCCC ATCCCCTCTG CTGGGGCACT CACTTGGCAA TTCCAAGATG
    GCTCAAGCCT TTCCCAGCAA AATGCACTCC CCACCCACCA TCGTCAGGCA CGTCGTGAGG
    CCCAAGAGTG CAGAACCCCC TCGGTCCCCG CTGCTCAAGC GCGTGCAGTC GGAGGAGAAG
    CTGTCTCCCT CCTATGGAGG CGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTTGAG
    GTCACCCAGG AGGAGGTGCC GCGGGAGCAG CAACAGCGGG AGGTGACCTT GCAGAGCCTG
    GAGGAGAACG TGTGCGACGC GCCTGCCCTC AGCCGGGCCA GGCCCGTGGA GCAGGGCTGC
    CTGAAACGCC CCGTGTCCCG GAAGCTGGGC CGCCAGGAGT CTGTGGACGA GCTGGACCGA
    GAGAAGCTAA AGGCCAAGGT GGTGGTGAAG AAACCTGACG GGCTCTCGGA AAAACAGGAA
    TCCCGCCCGA AGCCCCACGG ACTCGGCAGT GACTTGGAAA ACCTCTGTGC TTTTAGGCTC
    GAAGAGAGAG ACAAGAAAAT CTATCCAAAA GCTTCAGAGA GGTCAAATCA TTTTGAAAGC
    AAAGCAGCCG TGCAGGAGGC CCAGCCCCTG TGCAGCCTTT TGAAAGATGC TCTCCACAAG
    CAGGCCAATG TGCGGGCCAG CGAGGGTGTC GCCTCTGAGG GGGCAACGGC AGCTGGGGAC
    CACGGCCAGG GCACCTGTGA CTTCAAACGA GCCTCAACGC ACAGCACCCT CCAAGACGGC
    CTCTGTCACA CCACCCACAG GTCCACCTCC GGGAAGGGGG ACACCGCAGA GAAGGCCCCC
    CAGGCCAAGG AGGGTCTGCG GTGTGAGAAG TTAGACGGCA AGTTGGCCAA CATCGATTAC
    CTCCGAAAGA AGATGTCCCT TGAAGACAAA GACGAGAGCC TCTGTTCTGT GCTCAAGCCC
    AAGGTGACAG CTGGTGCCCA GGACTGCCTG CCTGGGAGCC TGGGCCGGCC CGTGGGAGGG
    CAGCAGGAGG CCCCGCCAGC CTCCGAGGCC CGCACACCGT TCATCGGCAG CGCCCACGCG
    GCTCAGCTCA GCGCCGTCTC CTTTGTCCCT CTCAAGGCCC TTTCTGGCCG GGTGGACAGT
    GGAGCGGAGA AGCCGGGCTT GGCTGCCCCA GAGTCGCCTG TCCGGAAAAG CCCCTCCGAG
    TATAAGCTGG AAGGGCGGTC TGTGTCGTGC CTGAAGCCCA TTGAAGGCAC CTTGGACATC
    GCTCTCCTGT CGGGACCTCA GGCCTCCAAG ACAGAGCTGC CTTCCCCGGA GCCTGCCCAG
    AGCCCCAGCC CAGGCAGTGA TGTGGAGTCC CCCATGCCCC TCGCTCTCCC CAGCAGCACA
    GGGAGGAAGG CTGACACTGC GGGTCAGAGA GAGTCCTCCC CTGCCGGCTT CAAGGTGAAC
    AAATCCTACC TGCTTGAGCC CCGGTTCCCA CCGGCCAGCC GGGGTCTGCA GAGTTCACCG
    GTGGTTGCCC CGCCTGACCC AGAGCTGAAG CTGGACAAGA AGGTCTCCCA GGCAGCCCGT
    GCCCCAGCGA CCATCATGGA GAGCCATCCC CAGCGGGGAG AGGGCCACCC CAGCCAACAC
    CAAGACCACA GCCCCGACGT TAAGCTCCTT CCCTTCCCAG GGCAGAACCT CCAAGGTGCC
    AACACGTCTA GATCCCGCAG CCCACTCTTG CCTGAAAGTG TCCCCCCTAG AGAGAAGCCG
    AGTGGGAGAG AGTTGTCCGA AAGGGGCCCT TCCATAGTCG GGAGCGAGTG GTCAGCTGTG
    AGGCCCGACA TCCGCAGAGA CCCCTCCAAG GAGCTGTGTC CCCCAGAAGC TGCTAAACCC
    AGCGACAACT CCCAAACCCC CCCTGTGGTG GGGAGGACCC GCCTGGATGT CTCCACCCAG
    ACCCAGGCCT TGGAGAAAGC ATGCGGGCCT TGCGGGAGAG CAAAGCCCAG AGACGGCCGA
    GAGGAGGTGA GGCTGCCGGC CAGAGAGGAC TCCTCTTTGC GCTCTGCGGG GGCTCCTTGT
    GAGAGAGAGC TGGGCGCAGA GAGAGGCCTG GAATCCAAGA TGGAACCTGC TTCTACCAGG
    TGGTCTCTGG AGCCGCCCAG GCCAGAGAGT GAGAAGAGTG GAAGGCCTTC TGGTTTCCAG
    TCTGTGCAGA AAGACGGTCC CAAGGAACCG GAGAGGAAAG AACAACCTCT GCAAAAGTAC
    CTCACCAGTT CTCAGCCGGG TCCCAAGGAA CCCGAGAGGA AAGAACAGCC CCTGCAAAAG
    CACCTCCCCA GTAGTTCTCA GCCGGGTCCC AAGGAACCCG AGAGGAAAGA ACAGCCCCTG
    CAAAAGCACG TCACCAGTAG TTCTCAGCCG GGTCCCAAGG AACCGGAGAG GAAAGAACAG
    CCTCTGCAAA AGCACCTCCC CAGTAGTTCT CAGCCGGGTC CCAAGGAACC GGAGAGGAAA
    GAACAGCCCC TGCAAAAGCA CGTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCCGAG
    AGGAAAGAAC AGCCTCTGCA AAAGCACCTC CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CTGGAAAGGA AAGAACAGTG TTTGCAGAAG CACCTCACCA GTAGCTCTCA GCTGGGTCCC
    AAGGAACCAG AAATGAAAGA ACAGCCCCCA CAAAAGCACG TCACCAGTAG TTCTCAACTG
    TGTCCCAAGG AACTGGAAAG GAAAGAACAA CCTCCACAAA AGCACCTCAC TATTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAGAGCA CGTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAACCGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACTTC
    ACCAGTAGTT CTCAGCTGGG TCCCAAGGAA CCAGAAACGA AAGAACAGCC TCTGCAAAAG
    CACCTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCGG AAAGGAAAGA ACAGCTTCTG
    CAAGAGCACC TCACCAGTAG CTCTCAGCTG AGTCCCAAGG AACCGGAAAG GAAAGAACAG
    TGTTTGCAGA AGCACCTCAC CAGTAGTTCT CAGCTGGGTC CCAAGGAACC AGAAATGAAA
    GAACAGCCTC TGCAAAAGCA CCTCACCAGT AGCTCTCAGC TGAGTCCCAA GGAACCAGAA
    AGGAAAGAAC AGCCTCTGCA AAAGCACGTC ACCAGTAGCT CTCAGCTGAG CCCCAAGGAG
    CCGGAAAGGA AAGAACAGCC TCTGCAAAAG CACCTCACCA GTAGCTGTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACG TCACCAGTAG CTCTCAGCTG
    AGCCCCAAGG AGCCAGAAAG GAAAGAACAG CCTCTGCAAA AGCACCTCAC CATTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGTCCCAA GGAGCCAGAA AGGAAAGAAC AGCTTCTGCA AAAGCACCTC
    ACCATTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCT TCTGCAAAAG
    CACCTCACCA TTAGCTCTCA GCTGAGTCCC AAGGAGCCGG AAAAGAAAGA ACAGCCTCTG
    CAAAAGCACG TCACCAGTAG CTCTCAGCTG AGTCCCAAGG AGCCGGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CATTAGCTCT CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA
    GAACAGCCTC TGCAAAAGCA CGTCACCAGT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCTTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAGGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACG TCACCAGTAG CTCTCAGCTG
    AGTCCCAAGG AACCGGAAAG GAAAGAACAG TATTTGCAGA AGCACCTCAC CAGTAGTTCT
    CAGCTGGGTC CCAAGGAACC AGAAACGAAA GAACAGCCTC TGCAAAAGCA CCTCACCAGT
    AGTTCTCAGG TGGGTCCCAA GGATCCGGAA AGGAAAGAAC AGCCCCCACA AAAGCACCTC
    ACCAGTAAGT CTCAGCCGGG TCCCAAGGAA CCGGAAAGGA AAGAACAGTG TTTGCAAAAG
    CACCTCACCA GTAGTTCTCA GCCGGGTCCC AAGGAACTGG AAAGGAAAGA ACAACCTCCA
    CTAAAGCATG TCACCAGTAG CTCTCAGCTG GGTCCCAGGG AACCAGAAAG GAAAGAACAG
    CCCCCACAAA AGCACCTCAC CAGTAGCTGT CAGCCCCCAC CCCCTACCAA AGAGCTCCCT
    GGCCTGGCTT GTCAGCACTG CATTCTGCCC AGCCACCCTG CTGCCTCTCT GCTGGGGAGC
    AAGCCCTGTG CTATAGACTC CAGCTTCCAG GATGCTCTCA GGCCCACTGC TGTGCGCAGT
    GACAGCAGCA GCCACAAGCC CAGGTCTGAC CCCGACCTGG GCCCTCCAAA GTCTAAGTTC
    CCAGACCGGC CCCTCTCCTC CCAGAAGCCG AGCGCTGAGG CTGCAGTGAG CAAAGAGCCT
    GGCGCTCCGA CCCTCGGCAG AGCTGGGGTG GGGAGTGGTA AGGGTGTGTC AGAGGAGTTC
    CCTGTCCTTC CAGATCCCCA GAACACAGCC CGTGAAGTGA TTGGCCAGGG AGCCAGTGGG
    TACTCAGGCC CCCTGCCCAT GGAAGGGGGT CCTCTAGACA CAAAGCTGAG ACCCACCAGC
    GCTCGGTGTC CCCCAGAGGC ACTGGAGATG CTTGGACATC CACCAGGGCG AGGGCTCCTA
    GGGGCCAGTG AGTCAGTGGA CCAGAAACTC CCCACCTTCA GGGAAAGGCA AAACCTGTCT
    CCAAAGGCCC CCACACCATC CACTGTGAAA GATTGTCCCC TTCCGTGCAA ACAGACAGAC
    AGGAGCCCGA GTCCTCTGGC CACCAGCACC AACGCTGGCA AGTCTGAAGG CAAGAAATGC
    ACTGAAGCAC TTTACACCCC TGCGGATGGT GAGAAGCTGG GGGCCGGCTT GTCCTCGGTG
    CACAGCGAGG CTGGGCCTAA GGGCACAGAG AAGCCGGCAG CAGCCGCTGG GAAGGGCTGG
    CCAGAGGCCA AGGGGAAAGG ACTCAGTCCC CAGAAGCCAC TGACCGAGGC CAGCAAGCCC
    AGCGGCATGA AGCGGTCACC TTCAGCCACG GTGCAGAGCT CTCTCCGCTG TGCCACCCTC
    CCAGAGAAGT CTCTGAGCTA CTCCTGTGGC TTTCCCGAGG CCAGACCAGC AGTTCCAGAG
    ACTTCTACCA CCTCCAGCGA CACCTCCTCT GCCAAGGCTG CTGGGCCTGC AGCAGAATCC
    CCAGCCTCCA GCAGCAGGGA CCAGCAGAAA CCCCTGCCTG GTGGGGATGG CAGAACCCAA
    ATGACCAAGA GCGACTCCTT GCCATGCTTC CGCAGCTCAG CCATCACTCT GGAGTCCCAG
    CACCCCAGCC CTGGCCTGGT GGTGGTCACC AGCCACCGGG ACAGGGCCCT CTCAGTAACC
    GCCACCGTGG GAGAAACCAA AGGGAAAGAG CCAGCCCCCA CCCAGCCAGC TCAGACCAGG
    AACCAGAACG TGAGCAGAGA GGCGGCCAAG CCGTCCCCCG CTCCAAGCAC TGAGCGCCCC
    ATCGTTCTTC CTTCTGAGAA GGACACTGGG CTGCGACAGA GGCGGGGGAA AGAGAGTTTG
    CGCGGCAGCC CCCACAAAAA GGCCTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228115
    Clone ID Related Accession (Same CDS sequence) XM_024981449.1
    Accession Version XM_024981449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
    AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CCAGCAACCG GAAAAGCTTG
    ATAGGCAATG GACCATCCCC AGCATTGCCT CGGCCCCACT CACCTCTTTC TGCTCATGCA
    GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCTGC TCATTTTTCA
    TTTGCACGGA GGACTGATGG ACGCCGCTGG TCCTTGGCTT CTCTCCCCTC CTCTGGCTAT
    GGGACAAACA CTCCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT
    CAGTTACCGT ATCAACCGAC ACCAGATGAA TTACACTTCT TATCAAAACA TTTTTGTACC
    ACTGAAAGCA TCACCACAGA GAACAGATGC AGAAACACAC CCATGCGCCC CCGTTCCCGA
    AGTTTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT
    GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAGCGCCT AAAGGAGATC
    ATCACCAGCT ACTCTCCAGA CCACGTTCTC CCCCTTGCAG ATGGAGTGCT CAGCTTCACT
    CACCATCAGA TTATCGAACT GGCTCGCGAT TGTTTGGATA AATCCCACCA GGGTCTCATC
    ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTACTACA GGAGGCTCAC
    AATCGTTCAG AAAGTGGAGA GCTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTGATT
    GTTATTGCCC GCCCTGCTCG CTTATTAGAG TGCCTGGAAT TTGATCCTGA AGAATTTTAC
    TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACTGACATT
    CCCAGGTACA TCATTAGCCA GCTTGGCCTC AATAAAGATC CTCTGGAAGA AATGGCTCAA
    CTGGGAAACT ATGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC
    TCTAATGCTT CCCTGAAACT TCGAAGGAAG CCTCGGGAAA GTGATTTTGA AACGATTAAA
    TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TATTTCGTTC GGCATAAGGA ATCCCGGCAG
    AGGTTTGCCA TGAAGAAGAT TAACAAACAG AACCTCATCC TCCGAAACCA GATCCAGCAG
    GCCTTTGTGG AACGGGATAT CCTGACTTTT GCAGAAAACC CTTTTGTCGT CAGCATGTAC
    TGCTCCTTTG AGACCAGGCG CCACTTGTGC ATGGTCATGG AGTATGTGGA AGGGGGTGAC
    TGTGCCACGT TAATGAAGAA CATGGGTCCT CTCCCCGTAG ATATGGCCAG GATGTACTTT
    GCTGAGACGG TCCTGGCCTT GGAATATCTA CATAATTATG GAATTGTACA TAGGGATTTG
    AAACCAGACA ATTTGCTGGT CACCTCTATG GGGCACATAA AGCTGACCGA TTTTGGATTA
    TCCAAGGTGG GACTCATGAG CATGACTACC AATCTTTATG AGGGTCATAT TGAGAAGGAT
    GCCCGAGAGT TCCTGGACAA GCAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG
    ATTCTGAGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT
    GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA
    GTCATCAGTG ATGAGATCAA CTGGCCTGAA AAGGATGAGG CTCCACCCCC TGATGCCCAG
    GATCTGATTA CCTTGCTCCT CAGGCAGAAT CCCCTGGAGA GACTGGGAAC AGGTGGTGCA
    TATGAAGTCA AACAGCATCG ATTCTTCCGT TGCTTAGACT GGAACAGTTT GCTGAGACAG
    AAGGCAGAAT TCATTCCCCA ACTGGAATCT GAAGACGACA CAAGTTATTT TGATACTCGG
    TCAGAGAAGT ACCATCATAT GGAGACTGAG GAAGAAGATG ACACGAACGA TGAAGACTTT
    AATGTGGAAA TAAGGCAGTT TTCCTCATGT TCACATAGGT TTTCTAAGGT CTTCAGCAGT
    ATGGATCGAG GTACTCAGAA TTCAGGAGAA GAGAAGGAAG ACCCTGGGGA CAAAACTAAA
    AGCACAGCTT TGCCGTCCAC GGAAACACTA AGCTGGAGTT CAGAGTATTC TGAAATGCAA
    CAGTTATCCA CATCCAACTC TTCAGACACT GAAAGCAACA GACATAGACG CGGTGCTGGG
    CTGCTTCCCA AGCTGGCTGT TTCAGCAGAG GCAGAACACG ACGGGGCCGC TCCCCGCCCC
    AGAGAGCTGC ACGAGGAGCC GGAAAAGCCA GCCCTGCCTG CTGCAGAGAA TGCTCAGGAG
    GAGCCCGAGG TCACTACCCC GGCCAGCACC ATCAGCAGCT CCACTCTGTC AGTTGGCAGT
    TTTTCAGAGC ACTTGGATCA GATAAATGGA CGGAGCGAGT GTGTGGACAG TACAGACAAT
    GCCTCCAAGC CCTCCAATGA GCCCGCCTCT CACATGGCTC GGCAGCGCTT AGAAAGCACA
    GAGAAACAGA AAGCCTCGGG GAAAGTCACA AAGTCCCTTT CTGCCAGTGC CCTGTCCCTC
    ATGATCCCAG GAGATATGTT TGCTGTCTCT CCGCTGGGAA GTCCGATGTC TCCCCATTCC
    CTGTCCTCGG ACCCTTCTTC CTCAAGGGAT TCCTCTCCCA GCCGAGATTC TTCAGGAGCT
    TCTGCCAGTC CACACCAGCC CATTGTGATC CACAGTTTGG GGAAGAACTA TGGTTTCACC
    ATTCGAGCCA TCCGGGTGTA CGTGGGAGAC AGCGACATTT ACACAGTGCA TCATATCGTT
    TGGAATGTAG AAGAAGGAAG TCCAGCTTGC CAGGCAGGAC TGAAAGCTGG GGATCTGATC
    ACACACATCA ATGGAGAACC GGTGCACGGA CTTGTCCACA CAGAAGTTAT AGAGCTCCTG
    CTAAAGAGTG GAAATAAGGT GTCAATCACG ACCACCCCAT TTGAAAACAC ATCAATCAAA
    ACGGGACCGG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCT
    AAGAAGAAGG AAAGTCTGGA AAGGAGGAGA TCCCTTTTCA AAAAACTAGC TAAGCAGCCT
    TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACCGATCCCT GTCGTCAGGG
    GAGAGCCTCC CAGGGTCCCC CACTCACAGC TTGTCTCCCC GATCCCCTAC ACCCAGCTAC
    CGTTCCACCC CCGACTTCCC ATCCGGCACT AACTCCTCCC AGAGCAGCTC CCCAAGCTCC
    AGCGCTCCCA ACTCCCCAGC AGGGTCAGGA CACGTCCGGC CCAGCACGCT CCATGGTCTG
    GCGCCCAAAC TCAGCGGGCA GCGCTACCGG TCAGGAAGGC GGAAGTCAGC TGGCAGCATC
    CCCCTGTCTC CGCTGGCCCG GACACCCTCT CCGACCCCAC AGCCCACCTC CCCGCAGCGG
    TCCCCATCCC CTCTGCTGGG GCACTCACTT GGCAATTCCA AGATGGCTCA AGCCTTTCCC
    AGCAAAATGC ACTCCCCACC CACCATCGTC AGGCACGTCG TGAGGCCCAA GAGTGCAGAA
    CCCCCTCGGT CCCCGCTGCT CAAGCGCGTG CAGTCGGAGG AGAAGCTGTC TCCCTCCTAT
    GGAGGCGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TTGAGGTCAC CCAGGAGGAG
    GTGCCGCGGG AGCAGCAACA GCGGGAGGTG ACCTTGCAGA GCCTGGAGGA GAACGTGTGC
    GACGCGCCTG CCCTCAGCCG GGCCAGGCCC GTGGAGCAGG GCTGCCTGAA ACGCCCCGTG
    TCCCGGAAGC TGGGCCGCCA GGAGTCTGTG GACGAGCTGG ACCGAGAGAA GCTAAAGGCC
    AAGGTGGTGG TGAAGAAACC TGACGGGCTC TCGGAAAAAC AGGAATCCCG CCCGAAGCCC
    CACGGACTCG GCAGTGACTT GGAAAACCTC TGTGCTTTTA GGCTCGAAGA GAGAGACAAG
    AAAATCTATC CAAAAGCTTC AGAGAGGTCA AATCATTTTG AAAGCAAAGC AGCCGTGCAG
    GAGGCCCAGC CCCTGTGCAG CCTTTTGAAA GATGCTCTCC ACAAGCAGGC CAATGTGCGG
    GCCAGCGAGG GTGTCGCCTC TGAGGGGGCA ACGGCAGCTG GGGACCACGG CCAGGGCACC
    TGTGACTTCA AACGAGCCTC AACGCACAGC ACCCTCCAAG ACGGCCTCTG TCACACCACC
    CACAGGTCCA CCTCCGGGAA GGGGGACACC GCAGAGAAGG CCCCCCAGGC CAAGGAGGGT
    CTGCGGTGTG AGAAGTTAGA CGGCAAGTTG GCCAACATCG ATTACCTCCG AAAGAAGATG
    TCCCTTGAAG ACAAAGACGA GAGCCTCTGT TCTGTGCTCA AGCCCAAGGT GACAGCTGGT
    GCCCAGGACT GCCTGCCTGG GAGCCTGGGC CGGCCCGTGG GAGGGCAGCA GGAGGCCCCG
    CCAGCCTCCG AGGCCCGCAC ACCGTTCATC GGCAGCGCCC ACGCGGCTCA GCTCAGCGCC
    GTCTCCTTTG TCCCTCTCAA GGCCCTTTCT GGCCGGGTGG ACAGTGGAGC GGAGAAGCCG
    GGCTTGGCTG CCCCAGAGTC GCCTGTCCGG AAAAGCCCCT CCGAGTATAA GCTGGAAGGG
    CGGTCTGTGT CGTGCCTGAA GCCCATTGAA GGCACCTTGG ACATCGCTCT CCTGTCGGGA
    CCTCAGGCCT CCAAGACAGA GCTGCCTTCC CCGGAGCCTG CCCAGAGCCC CAGCCCAGGC
    AGTGATGTGG AGTCCCCCAT GCCCCTCGCT CTCCCCAGCA GCACAGGGAG GAAGGCTGAC
    ACTGCGGGTC AGAGAGAGTC CTCCCCTGCC GGCTTCAAGG TGAACAAATC CTACCTGCTT
    GAGCCCCGGT TCCCACCGGC CAGCCGGGGT CTGCAGAGTT CACCGGTGGT TGCCCCGCCT
    GACCCAGAGC TGAAGCTGGA CAAGAAGGTC TCCCAGGCAG CCCGTGCCCC AGCGACCATC
    ATGGAGAGCC ATCCCCAGCG GGGAGAGGGC CACCCCAGCC AACACCAAGA CCACAGCCCC
    GACGTTAAGC TCCTTCCCTT CCCAGGGCAG AACCTCCAAG GTGCCAACAC GTCTAGATCC
    CGCAGCCCAC TCTTGCCTGA AAGTGTCCCC CCTAGAGAGA AGCCGAGTGG GAGAGAGTTG
    TCCGAAAGGG GCCCTTCCAT AGTCGGGAGC GAGTGGTCAG CTGTGAGGCC CGACATCCGC
    AGAGACCCCT CCAAGGAGCT GTGTCCCCCA GAAGCTGCTA AACCCAGCGA CAACTCCCAA
    ACCCCCCCTG TGGTGGGGAG GACCCGCCTG GATGTCTCCA CCCAGACCCA GGCCTTGGAG
    AAAGCATGCG GGCCTTGCGG GAGAGCAAAG CCCAGAGACG GCCGAGAGGA GGTGAGGCTG
    CCGGCCAGAG AGGACTCCTC TTTGCGCTCT GCGGGGGCTC CTTGTGAGAG AGAGCTGGGC
    GCAGAGAGAG GCCTGGAATC CAAGATGGAA CCTGCTTCTA CCAGGTGGTC TCTGGAGCCG
    CCCAGGCCAG AGAGTGAGAA GAGTGGAAGG CCTTCTGGTT TCCAGTCTGT GCAGAAAGAC
    GGTCCCAAGG AACCGGAGAG GAAAGAACAA CCTCTGCAAA AGTACCTCAC CAGTTCTCAG
    CCGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCCCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG AAAGAACAGC CCCTGCAAAA GCACGTCACC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACCG GAGAGGAAAG AACAGCCTCT GCAAAAGCAC
    CTCCCCAGTA GTTCTCAGCC GGGTCCCAAG GAACCGGAGA GGAAAGAACA GCCCCTGCAA
    AAGCACGTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CCGAGAGGAA AGAACAGCCT
    CTGCAAAAGC ACCTCCCCAG TAGTTCTCAG CCGGGTCCCA AGGAACTGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACCT CACCAGTAGC TCTCAGCTGG GTCCCAAGGA ACCAGAAATG
    AAAGAACAGC CCCCACAAAA GCACGTCACC AGTAGTTCTC AACTGTGTCC CAAGGAACTG
    GAAAGGAAAG AACAACCTCC ACAAAAGCAC CTCACTATTA GCTCTCAGCT GAGTCCCAAG
    GAACCAGAAA GGAAAGAACA GCCTCTGCAA GAGCACGTCA CCAGTAGCTC TCAGCTGAGT
    CCCAAGGAAC CGGAAAGGAA AGAACAGTGT TTGCAGAAGC ACTTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCAGA AACGAAAGAA CAGCCTCTGC AAAAGCACCT CACCAGTAGC
    TCTCAGCTGA GTCCCAAGGA ACCGGAAAGG AAAGAACAGC TTCTGCAAGA GCACCTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAACCG GAAAGGAAAG AACAGTGTTT GCAGAAGCAC
    CTCACCAGTA GTTCTCAGCT GGGTCCCAAG GAACCAGAAA TGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGCCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACCT CACCAGTAGC TGTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGCCC CAAGGAGCCA
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC CTCACCATTA GCTCTCAGCT GAGTCCCAAG
    GAACCAGAAA GGAAAGAACA GCCTCTGCAA AAGCACGTCA CCAGTAGCTC TCAGCTGAGT
    CCCAAGGAGC CAGAAAGGAA AGAACAGCTT CTGCAAAAGC ACCTCACCAT TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAAG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    CTCACCATTA GCTCTCAGCT GAGTCCCAAG GAGCCGGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACGTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCTT
    CTGCAAAAGC ACCTCACCAT TAGCTCTCAG CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACGT CACCAGTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGTATTT GCAGAAGCAC CTCACCAGTA GTTCTCAGCT GGGTCCCAAG
    GAACCAGAAA CGAAAGAACA GCCTCTGCAA AAGCACCTCA CCAGTAGTTC TCAGGTGGGT
    CCCAAGGATC CGGAAAGGAA AGAACAGCCC CCACAAAAGC ACCTCACCAG TAAGTCTCAG
    CCGGGTCCCA AGGAACCGGA AAGGAAAGAA CAGTGTTTGC AAAAGCACCT CACCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAAC CTCCACTAAA GCATGTCACC
    AGTAGCTCTC AGCTGGGTCC CAGGGAACCA GAAAGGAAAG AACAGCCCCC ACAAAAGCAC
    CTCACCAGTA GCTGTCAGCC CCCACCCCCT ACCAAAGAGC TCCCTGGCCT GGCTTGTCAG
    CACTGCATTC TGCCCAGCCA CCCTGCTGCC TCTCTGCTGG GGAGCAAGCC CTGTGCTATA
    GACTCCAGCT TCCAGGATGC TCTCAGGCCC ACTGCTGTGC GCAGTGACAG CAGCAGCCAC
    AAGCCCAGGT CTGACCCCGA CCTGGGCCCT CCAAAGTCTA AGTTCCCAGA CCGGCCCCTC
    TCCTCCCAGA AGCCGAGCGC TGAGGCTGCA GTGAGCAAAG AGCCTGGCGC TCCGACCCTC
    GGCAGAGCTG GGGTGGGGAG TGGTAAGGGT GTGTCAGAGG AGTTCCCTGT CCTTCCAGAT
    CCCCAGAACA CAGCCCGTGA AGTGATTGGC CAGGGAGCCA GTGGGTACTC AGGCCCCCTG
    CCCATGGAAG GGGGTCCTCT AGACACAAAG CTGAGACCCA CCAGCGCTCG GTGTCCCCCA
    GAGGCACTGG AGATGCTTGG ACATCCACCA GGGCGAGGGC TCCTAGGGGC CAGTGAGTCA
    GTGGACCAGA AACTCCCCAC CTTCAGGGAA AGGCAAAACC TGTCTCCAAA GGCCCCCACA
    CCATCCACTG TGAAAGATTG TCCCCTTCCG TGCAAACAGA CAGACAGGAG CCCGAGTCCT
    CTGGCCACCA GCACCAACGC TGGCAAGTCT GAAGGCAAGA AATGCACTGA AGCACTTTAC
    ACCCCTGCGG ATGGTGAGAA GCTGGGGGCC GGCTTGTCCT CGGTGCACAG CGAGGCTGGG
    CCTAAGGGCA CAGAGAAGCC GGCAGCAGCC GCTGGGAAGG GCTGGCCAGA GGCCAAGGGG
    AAAGGACTCA GTCCCCAGAA GCCACTGACC GAGGCCAGCA AGCCCAGCGG CATGAAGCGG
    TCACCTTCAG CCACGGTGCA GAGCTCTCTC CGCTGTGCCA CCCTCCCAGA GAAGTCTCTG
    AGCTACTCCT GTGGCTTTCC CGAGGCCAGA CCAGCAGTTC CAGAGACTTC TACCACCTCC
    AGCGACACCT CCTCTGCCAA GGCTGCTGGG CCTGCAGCAG AATCCCCAGC CTCCAGCAGC
    AGGGACCAGC AGAAACCCCT GCCTGGTGGG GATGGCAGAA CCCAAATGAC CAAGAGCGAC
    TCCTTGCCAT GCTTCCGCAG CTCAGCCATC ACTCTGGAGT CCCAGCACCC CAGCCCTGGC
    CTGGTGGTGG TCACCAGCCA CCGGGACAGG GCCCTCTCAG TAACCGCCAC CGTGGGAGAA
    ACCAAAGGGA AAGAGCCAGC CCCCACCCAG CCAGCTCAGA CCAGGAACCA GAACGTGAGC
    AGAGAGGCGG CCAAGCCGTC CCCCGCTCCA AGCACTGAGC GCCCCATCGT TCTTCCTTCT
    GAGAAGGACA CTGGGCTGCG ACAGAGGCGG GGGAAAGAGA GTTTGCGCGG CAGCCCCCAC
    AAAAAGGCCT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837217.1
    CDS344..9778
    Translation

    Target ORF information:

    RefSeq Version XM_024981449.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
    AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CCAGCAACCG GAAAAGCTTG
    ATAGGCAATG GACCATCCCC AGCATTGCCT CGGCCCCACT CACCTCTTTC TGCTCATGCA
    GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCTGC TCATTTTTCA
    TTTGCACGGA GGACTGATGG ACGCCGCTGG TCCTTGGCTT CTCTCCCCTC CTCTGGCTAT
    GGGACAAACA CTCCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT
    CAGTTACCGT ATCAACCGAC ACCAGATGAA TTACACTTCT TATCAAAACA TTTTTGTACC
    ACTGAAAGCA TCACCACAGA GAACAGATGC AGAAACACAC CCATGCGCCC CCGTTCCCGA
    AGTTTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT
    GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAGCGCCT AAAGGAGATC
    ATCACCAGCT ACTCTCCAGA CCACGTTCTC CCCCTTGCAG ATGGAGTGCT CAGCTTCACT
    CACCATCAGA TTATCGAACT GGCTCGCGAT TGTTTGGATA AATCCCACCA GGGTCTCATC
    ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTACTACA GGAGGCTCAC
    AATCGTTCAG AAAGTGGAGA GCTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTGATT
    GTTATTGCCC GCCCTGCTCG CTTATTAGAG TGCCTGGAAT TTGATCCTGA AGAATTTTAC
    TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACTGACATT
    CCCAGGTACA TCATTAGCCA GCTTGGCCTC AATAAAGATC CTCTGGAAGA AATGGCTCAA
    CTGGGAAACT ATGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC
    TCTAATGCTT CCCTGAAACT TCGAAGGAAG CCTCGGGAAA GTGATTTTGA AACGATTAAA
    TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TATTTCGTTC GGCATAAGGA ATCCCGGCAG
    AGGTTTGCCA TGAAGAAGAT TAACAAACAG AACCTCATCC TCCGAAACCA GATCCAGCAG
    GCCTTTGTGG AACGGGATAT CCTGACTTTT GCAGAAAACC CTTTTGTCGT CAGCATGTAC
    TGCTCCTTTG AGACCAGGCG CCACTTGTGC ATGGTCATGG AGTATGTGGA AGGGGGTGAC
    TGTGCCACGT TAATGAAGAA CATGGGTCCT CTCCCCGTAG ATATGGCCAG GATGTACTTT
    GCTGAGACGG TCCTGGCCTT GGAATATCTA CATAATTATG GAATTGTACA TAGGGATTTG
    AAACCAGACA ATTTGCTGGT CACCTCTATG GGGCACATAA AGCTGACCGA TTTTGGATTA
    TCCAAGGTGG GACTCATGAG CATGACTACC AATCTTTATG AGGGTCATAT TGAGAAGGAT
    GCCCGAGAGT TCCTGGACAA GCAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG
    ATTCTGAGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT
    GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA
    GTCATCAGTG ATGAGATCAA CTGGCCTGAA AAGGATGAGG CTCCACCCCC TGATGCCCAG
    GATCTGATTA CCTTGCTCCT CAGGCAGAAT CCCCTGGAGA GACTGGGAAC AGGTGGTGCA
    TATGAAGTCA AACAGCATCG ATTCTTCCGT TGCTTAGACT GGAACAGTTT GCTGAGACAG
    AAGGCAGAAT TCATTCCCCA ACTGGAATCT GAAGACGACA CAAGTTATTT TGATACTCGG
    TCAGAGAAGT ACCATCATAT GGAGACTGAG GAAGAAGATG ACACGAACGA TGAAGACTTT
    AATGTGGAAA TAAGGCAGTT TTCCTCATGT TCACATAGGT TTTCTAAGGT CTTCAGCAGT
    ATGGATCGAG GTACTCAGAA TTCAGGAGAA GAGAAGGAAG ACCCTGGGGA CAAAACTAAA
    AGCACAGCTT TGCCGTCCAC GGAAACACTA AGCTGGAGTT CAGAGTATTC TGAAATGCAA
    CAGTTATCCA CATCCAACTC TTCAGACACT GAAAGCAACA GACATAGACG CGGTGCTGGG
    CTGCTTCCCA AGCTGGCTGT TTCAGCAGAG GCAGAACACG ACGGGGCCGC TCCCCGCCCC
    AGAGAGCTGC ACGAGGAGCC GGAAAAGCCA GCCCTGCCTG CTGCAGAGAA TGCTCAGGAG
    GAGCCCGAGG TCACTACCCC GGCCAGCACC ATCAGCAGCT CCACTCTGTC AGTTGGCAGT
    TTTTCAGAGC ACTTGGATCA GATAAATGGA CGGAGCGAGT GTGTGGACAG TACAGACAAT
    GCCTCCAAGC CCTCCAATGA GCCCGCCTCT CACATGGCTC GGCAGCGCTT AGAAAGCACA
    GAGAAACAGA AAGCCTCGGG GAAAGTCACA AAGTCCCTTT CTGCCAGTGC CCTGTCCCTC
    ATGATCCCAG GAGATATGTT TGCTGTCTCT CCGCTGGGAA GTCCGATGTC TCCCCATTCC
    CTGTCCTCGG ACCCTTCTTC CTCAAGGGAT TCCTCTCCCA GCCGAGATTC TTCAGGAGCT
    TCTGCCAGTC CACACCAGCC CATTGTGATC CACAGTTTGG GGAAGAACTA TGGTTTCACC
    ATTCGAGCCA TCCGGGTGTA CGTGGGAGAC AGCGACATTT ACACAGTGCA TCATATCGTT
    TGGAATGTAG AAGAAGGAAG TCCAGCTTGC CAGGCAGGAC TGAAAGCTGG GGATCTGATC
    ACACACATCA ATGGAGAACC GGTGCACGGA CTTGTCCACA CAGAAGTTAT AGAGCTCCTG
    CTAAAGAGTG GAAATAAGGT GTCAATCACG ACCACCCCAT TTGAAAACAC ATCAATCAAA
    ACGGGACCGG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCT
    AAGAAGAAGG AAAGTCTGGA AAGGAGGAGA TCCCTTTTCA AAAAACTAGC TAAGCAGCCT
    TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACCGATCCCT GTCGTCAGGG
    GAGAGCCTCC CAGGGTCCCC CACTCACAGC TTGTCTCCCC GATCCCCTAC ACCCAGCTAC
    CGTTCCACCC CCGACTTCCC ATCCGGCACT AACTCCTCCC AGAGCAGCTC CCCAAGCTCC
    AGCGCTCCCA ACTCCCCAGC AGGGTCAGGA CACGTCCGGC CCAGCACGCT CCATGGTCTG
    GCGCCCAAAC TCAGCGGGCA GCGCTACCGG TCAGGAAGGC GGAAGTCAGC TGGCAGCATC
    CCCCTGTCTC CGCTGGCCCG GACACCCTCT CCGACCCCAC AGCCCACCTC CCCGCAGCGG
    TCCCCATCCC CTCTGCTGGG GCACTCACTT GGCAATTCCA AGATGGCTCA AGCCTTTCCC
    AGCAAAATGC ACTCCCCACC CACCATCGTC AGGCACGTCG TGAGGCCCAA GAGTGCAGAA
    CCCCCTCGGT CCCCGCTGCT CAAGCGCGTG CAGTCGGAGG AGAAGCTGTC TCCCTCCTAT
    GGAGGCGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TTGAGGTCAC CCAGGAGGAG
    GTGCCGCGGG AGCAGCAACA GCGGGAGGTG ACCTTGCAGA GCCTGGAGGA GAACGTGTGC
    GACGCGCCTG CCCTCAGCCG GGCCAGGCCC GTGGAGCAGG GCTGCCTGAA ACGCCCCGTG
    TCCCGGAAGC TGGGCCGCCA GGAGTCTGTG GACGAGCTGG ACCGAGAGAA GCTAAAGGCC
    AAGGTGGTGG TGAAGAAACC TGACGGGCTC TCGGAAAAAC AGGAATCCCG CCCGAAGCCC
    CACGGACTCG GCAGTGACTT GGAAAACCTC TGTGCTTTTA GGCTCGAAGA GAGAGACAAG
    AAAATCTATC CAAAAGCTTC AGAGAGGTCA AATCATTTTG AAAGCAAAGC AGCCGTGCAG
    GAGGCCCAGC CCCTGTGCAG CCTTTTGAAA GATGCTCTCC ACAAGCAGGC CAATGTGCGG
    GCCAGCGAGG GTGTCGCCTC TGAGGGGGCA ACGGCAGCTG GGGACCACGG CCAGGGCACC
    TGTGACTTCA AACGAGCCTC AACGCACAGC ACCCTCCAAG ACGGCCTCTG TCACACCACC
    CACAGGTCCA CCTCCGGGAA GGGGGACACC GCAGAGAAGG CCCCCCAGGC CAAGGAGGGT
    CTGCGGTGTG AGAAGTTAGA CGGCAAGTTG GCCAACATCG ATTACCTCCG AAAGAAGATG
    TCCCTTGAAG ACAAAGACGA GAGCCTCTGT TCTGTGCTCA AGCCCAAGGT GACAGCTGGT
    GCCCAGGACT GCCTGCCTGG GAGCCTGGGC CGGCCCGTGG GAGGGCAGCA GGAGGCCCCG
    CCAGCCTCCG AGGCCCGCAC ACCGTTCATC GGCAGCGCCC ACGCGGCTCA GCTCAGCGCC
    GTCTCCTTTG TCCCTCTCAA GGCCCTTTCT GGCCGGGTGG ACAGTGGAGC GGAGAAGCCG
    GGCTTGGCTG CCCCAGAGTC GCCTGTCCGG AAAAGCCCCT CCGAGTATAA GCTGGAAGGG
    CGGTCTGTGT CGTGCCTGAA GCCCATTGAA GGCACCTTGG ACATCGCTCT CCTGTCGGGA
    CCTCAGGCCT CCAAGACAGA GCTGCCTTCC CCGGAGCCTG CCCAGAGCCC CAGCCCAGGC
    AGTGATGTGG AGTCCCCCAT GCCCCTCGCT CTCCCCAGCA GCACAGGGAG GAAGGCTGAC
    ACTGCGGGTC AGAGAGAGTC CTCCCCTGCC GGCTTCAAGG TGAACAAATC CTACCTGCTT
    GAGCCCCGGT TCCCACCGGC CAGCCGGGGT CTGCAGAGTT CACCGGTGGT TGCCCCGCCT
    GACCCAGAGC TGAAGCTGGA CAAGAAGGTC TCCCAGGCAG CCCGTGCCCC AGCGACCATC
    ATGGAGAGCC ATCCCCAGCG GGGAGAGGGC CACCCCAGCC AACACCAAGA CCACAGCCCC
    GACGTTAAGC TCCTTCCCTT CCCAGGGCAG AACCTCCAAG GTGCCAACAC GTCTAGATCC
    CGCAGCCCAC TCTTGCCTGA AAGTGTCCCC CCTAGAGAGA AGCCGAGTGG GAGAGAGTTG
    TCCGAAAGGG GCCCTTCCAT AGTCGGGAGC GAGTGGTCAG CTGTGAGGCC CGACATCCGC
    AGAGACCCCT CCAAGGAGCT GTGTCCCCCA GAAGCTGCTA AACCCAGCGA CAACTCCCAA
    ACCCCCCCTG TGGTGGGGAG GACCCGCCTG GATGTCTCCA CCCAGACCCA GGCCTTGGAG
    AAAGCATGCG GGCCTTGCGG GAGAGCAAAG CCCAGAGACG GCCGAGAGGA GGTGAGGCTG
    CCGGCCAGAG AGGACTCCTC TTTGCGCTCT GCGGGGGCTC CTTGTGAGAG AGAGCTGGGC
    GCAGAGAGAG GCCTGGAATC CAAGATGGAA CCTGCTTCTA CCAGGTGGTC TCTGGAGCCG
    CCCAGGCCAG AGAGTGAGAA GAGTGGAAGG CCTTCTGGTT TCCAGTCTGT GCAGAAAGAC
    GGTCCCAAGG AACCGGAGAG GAAAGAACAA CCTCTGCAAA AGTACCTCAC CAGTTCTCAG
    CCGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCCCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG AAAGAACAGC CCCTGCAAAA GCACGTCACC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACCG GAGAGGAAAG AACAGCCTCT GCAAAAGCAC
    CTCCCCAGTA GTTCTCAGCC GGGTCCCAAG GAACCGGAGA GGAAAGAACA GCCCCTGCAA
    AAGCACGTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CCGAGAGGAA AGAACAGCCT
    CTGCAAAAGC ACCTCCCCAG TAGTTCTCAG CCGGGTCCCA AGGAACTGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACCT CACCAGTAGC TCTCAGCTGG GTCCCAAGGA ACCAGAAATG
    AAAGAACAGC CCCCACAAAA GCACGTCACC AGTAGTTCTC AACTGTGTCC CAAGGAACTG
    GAAAGGAAAG AACAACCTCC ACAAAAGCAC CTCACTATTA GCTCTCAGCT GAGTCCCAAG
    GAACCAGAAA GGAAAGAACA GCCTCTGCAA GAGCACGTCA CCAGTAGCTC TCAGCTGAGT
    CCCAAGGAAC CGGAAAGGAA AGAACAGTGT TTGCAGAAGC ACTTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCAGA AACGAAAGAA CAGCCTCTGC AAAAGCACCT CACCAGTAGC
    TCTCAGCTGA GTCCCAAGGA ACCGGAAAGG AAAGAACAGC TTCTGCAAGA GCACCTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAACCG GAAAGGAAAG AACAGTGTTT GCAGAAGCAC
    CTCACCAGTA GTTCTCAGCT GGGTCCCAAG GAACCAGAAA TGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGCCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACCT CACCAGTAGC TGTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGCCC CAAGGAGCCA
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC CTCACCATTA GCTCTCAGCT GAGTCCCAAG
    GAACCAGAAA GGAAAGAACA GCCTCTGCAA AAGCACGTCA CCAGTAGCTC TCAGCTGAGT
    CCCAAGGAGC CAGAAAGGAA AGAACAGCTT CTGCAAAAGC ACCTCACCAT TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAAG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    CTCACCATTA GCTCTCAGCT GAGTCCCAAG GAGCCGGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACGTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCTT
    CTGCAAAAGC ACCTCACCAT TAGCTCTCAG CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACGT CACCAGTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGTATTT GCAGAAGCAC CTCACCAGTA GTTCTCAGCT GGGTCCCAAG
    GAACCAGAAA CGAAAGAACA GCCTCTGCAA AAGCACCTCA CCAGTAGTTC TCAGGTGGGT
    CCCAAGGATC CGGAAAGGAA AGAACAGCCC CCACAAAAGC ACCTCACCAG TAAGTCTCAG
    CCGGGTCCCA AGGAACCGGA AAGGAAAGAA CAGTGTTTGC AAAAGCACCT CACCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAAC CTCCACTAAA GCATGTCACC
    AGTAGCTCTC AGCTGGGTCC CAGGGAACCA GAAAGGAAAG AACAGCCCCC ACAAAAGCAC
    CTCACCAGTA GCTGTCAGCC CCCACCCCCT ACCAAAGAGC TCCCTGGCCT GGCTTGTCAG
    CACTGCATTC TGCCCAGCCA CCCTGCTGCC TCTCTGCTGG GGAGCAAGCC CTGTGCTATA
    GACTCCAGCT TCCAGGATGC TCTCAGGCCC ACTGCTGTGC GCAGTGACAG CAGCAGCCAC
    AAGCCCAGGT CTGACCCCGA CCTGGGCCCT CCAAAGTCTA AGTTCCCAGA CCGGCCCCTC
    TCCTCCCAGA AGCCGAGCGC TGAGGCTGCA GTGAGCAAAG AGCCTGGCGC TCCGACCCTC
    GGCAGAGCTG GGGTGGGGAG TGGTAAGGGT GTGTCAGAGG AGTTCCCTGT CCTTCCAGAT
    CCCCAGAACA CAGCCCGTGA AGTGATTGGC CAGGGAGCCA GTGGGTACTC AGGCCCCCTG
    CCCATGGAAG GGGGTCCTCT AGACACAAAG CTGAGACCCA CCAGCGCTCG GTGTCCCCCA
    GAGGCACTGG AGATGCTTGG ACATCCACCA GGGCGAGGGC TCCTAGGGGC CAGTGAGTCA
    GTGGACCAGA AACTCCCCAC CTTCAGGGAA AGGCAAAACC TGTCTCCAAA GGCCCCCACA
    CCATCCACTG TGAAAGATTG TCCCCTTCCG TGCAAACAGA CAGACAGGAG CCCGAGTCCT
    CTGGCCACCA GCACCAACGC TGGCAAGTCT GAAGGCAAGA AATGCACTGA AGCACTTTAC
    ACCCCTGCGG ATGGTGAGAA GCTGGGGGCC GGCTTGTCCT CGGTGCACAG CGAGGCTGGG
    CCTAAGGGCA CAGAGAAGCC GGCAGCAGCC GCTGGGAAGG GCTGGCCAGA GGCCAAGGGG
    AAAGGACTCA GTCCCCAGAA GCCACTGACC GAGGCCAGCA AGCCCAGCGG CATGAAGCGG
    TCACCTTCAG CCACGGTGCA GAGCTCTCTC CGCTGTGCCA CCCTCCCAGA GAAGTCTCTG
    AGCTACTCCT GTGGCTTTCC CGAGGCCAGA CCAGCAGTTC CAGAGACTTC TACCACCTCC
    AGCGACACCT CCTCTGCCAA GGCTGCTGGG CCTGCAGCAG AATCCCCAGC CTCCAGCAGC
    AGGGACCAGC AGAAACCCCT GCCTGGTGGG GATGGCAGAA CCCAAATGAC CAAGAGCGAC
    TCCTTGCCAT GCTTCCGCAG CTCAGCCATC ACTCTGGAGT CCCAGCACCC CAGCCCTGGC
    CTGGTGGTGG TCACCAGCCA CCGGGACAGG GCCCTCTCAG TAACCGCCAC CGTGGGAGAA
    ACCAAAGGGA AAGAGCCAGC CCCCACCCAG CCAGCTCAGA CCAGGAACCA GAACGTGAGC
    AGAGAGGCGG CCAAGCCGTC CCCCGCTCCA AGCACTGAGC GCCCCATCGT TCTTCCTTCT
    GAGAAGGACA CTGGGCTGCG ACAGAGGCGG GGGAAAGAGA GTTTGCGCGG CAGCCCCCAC
    AAAAAGGCCT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228116
    Clone ID Related Accession (Same CDS sequence) XM_024981450.1
    Accession Version XM_024981450.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTTCCCAGA 
    AGAGGAAGTC TTTGCCGAAC CAGCAACCGG AAAAGCTTGA TAGGCAATGG ACCATCCCCA
    GCATTGCCTC GGCCCCACTC ACCTCTTTCT GCTCATGCAG GAAATAGCCC TCAAGATAGT
    CCAAGAAATT TCTCCCCCAG TGCCTCTGCT CATTTTTCAT TTGCACGGAG GACTGATGGA
    CGCCGCTGGT CCTTGGCTTC TCTCCCCTCC TCTGGCTATG GGACAAACAC TCCCAGCTCT
    ACGGTCTCTT CATCCTGTTC CTCCCAGGAG AAGTTGCATC AGTTACCGTA TCAACCGACA
    CCAGATGAAT TACACTTCTT ATCAAAACAT TTTTGTACCA CTGAAAGCAT CACCACAGAG
    AACAGATGCA GAAACACACC CATGCGCCCC CGTTCCCGAA GTTTGAGCCC TGGACGTTCT
    CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA
    AAGGCTACAG CTCAGATGGA AGAGCGCCTA AAGGAGATCA TCACCAGCTA CTCTCCAGAC
    CACGTTCTCC CCCTTGCAGA TGGAGTGCTC AGCTTCACTC ACCATCAGAT TATCGAACTG
    GCTCGCGATT GTTTGGATAA ATCCCACCAG GGTCTCATCA CCTCACGATA CTTCCTTGAA
    TTACAGCACA AATTAGATAA GTTACTACAG GAGGCTCACA ATCGTTCAGA AAGTGGAGAG
    CTGGCATTTA TTAAACAACT AGTTCGAAAG ATCCTGATTG TTATTGCCCG CCCTGCTCGC
    TTATTAGAGT GCCTGGAATT TGATCCTGAA GAATTTTACT ACCTATTGGA AGCAGCAGAA
    GGCCATGCCA AAGAAGGACA GGGTATTAAA ACTGACATTC CCAGGTACAT CATTAGCCAG
    CTTGGCCTCA ATAAAGATCC TCTGGAAGAA ATGGCTCAAC TGGGAAACTA TGATAGTGGG
    ACAGCAGAAA CACCAGAAAC AGATGAATCA GTGAGTAGCT CTAATGCTTC CCTGAAACTT
    CGAAGGAAGC CTCGGGAAAG TGATTTTGAA ACGATTAAAT TGATCAGCAA TGGAGCCTAC
    GGGGCAGTCT ATTTCGTTCG GCATAAGGAA TCCCGGCAGA GGTTTGCCAT GAAGAAGATT
    AACAAACAGA ACCTCATCCT CCGAAACCAG ATCCAGCAGG CCTTTGTGGA ACGGGATATC
    CTGACTTTTG CAGAAAACCC TTTTGTCGTC AGCATGTACT GCTCCTTTGA GACCAGGCGC
    CACTTGTGCA TGGTCATGGA GTATGTGGAA GGGGGTGACT GTGCCACGTT AATGAAGAAC
    ATGGGTCCTC TCCCCGTAGA TATGGCCAGG ATGTACTTTG CTGAGACGGT CCTGGCCTTG
    GAATATCTAC ATAATTATGG AATTGTACAT AGGGATTTGA AACCAGACAA TTTGCTGGTC
    ACCTCTATGG GGCACATAAA GCTGACCGAT TTTGGATTAT CCAAGGTGGG ACTCATGAGC
    ATGACTACCA ATCTTTATGA GGGTCATATT GAGAAGGATG CCCGAGAGTT CCTGGACAAG
    CAGGTCTGTG GCACACCTGA ATACATTGCA CCAGAAGTGA TTCTGAGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC CATGGGGATT ATCCTCTATG AATTTCTGGT TGGATGCGTG
    CCATTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAGATCAAC
    TGGCCTGAAA AGGATGAGGC TCCACCCCCT GATGCCCAGG ATCTGATTAC CTTGCTCCTC
    AGGCAGAATC CCCTGGAGAG ACTGGGAACA GGTGGTGCAT ATGAAGTCAA ACAGCATCGA
    TTCTTCCGTT GCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCAGAATT CATTCCCCAA
    CTGGAATCTG AAGACGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA CCATCATATG
    GAGACTGAGG AAGAAGATGA CACGAACGAT GAAGACTTTA ATGTGGAAAT AAGGCAGTTT
    TCCTCATGTT CACATAGGTT TTCTAAGGTC TTCAGCAGTA TGGATCGAGG TACTCAGAAT
    TCAGGAGAAG AGAAGGAAGA CCCTGGGGAC AAAACTAAAA GCACAGCTTT GCCGTCCACG
    GAAACACTAA GCTGGAGTTC AGAGTATTCT GAAATGCAAC AGTTATCCAC ATCCAACTCT
    TCAGACACTG AAAGCAACAG ACATAGACGC GGTGCTGGGC TGCTTCCCAA GCTGGCTGTT
    TCAGCAGAGG CAGAACACGA CGGGGCCGCT CCCCGCCCCA GAGAGCTGCA CGAGGAGCCG
    GAAAAGCCAG CCCTGCCTGC TGCAGAGAAT GCTCAGGAGG AGCCCGAGGT CACTACCCCG
    GCCAGCACCA TCAGCAGCTC CACTCTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG
    ATAAATGGAC GGAGCGAGTG TGTGGACAGT ACAGACAATG CCTCCAAGCC CTCCAATGAG
    CCCGCCTCTC ACATGGCTCG GCAGCGCTTA GAAAGCACAG AGAAACAGAA AGCCTCGGGG
    AAAGTCACAA AGTCCCTTTC TGCCAGTGCC CTGTCCCTCA TGATCCCAGG AGATATGTTT
    GCTGTCTCTC CGCTGGGAAG TCCGATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCC
    TCAAGGGATT CCTCTCCCAG CCGAGATTCT TCAGGAGCTT CTGCCAGTCC ACACCAGCCC
    ATTGTGATCC ACAGTTTGGG GAAGAACTAT GGTTTCACCA TTCGAGCCAT CCGGGTGTAC
    GTGGGAGACA GCGACATTTA CACAGTGCAT CATATCGTTT GGAATGTAGA AGAAGGAAGT
    CCAGCTTGCC AGGCAGGACT GAAAGCTGGG GATCTGATCA CACACATCAA TGGAGAACCG
    GTGCACGGAC TTGTCCACAC AGAAGTTATA GAGCTCCTGC TAAAGAGTGG AAATAAGGTG
    TCAATCACGA CCACCCCATT TGAAAACACA TCAATCAAAA CGGGACCGGC CAGGAGAAAC
    AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCTA AGAAGAAGGA AAGTCTGGAA
    AGGAGGAGAT CCCTTTTCAA AAAACTAGCT AAGCAGCCTT CTCCTTTACT CCACACCAGC
    CGAAGTTTCT CCTGCTTGAA CCGATCCCTG TCGTCAGGGG AGAGCCTCCC AGGGTCCCCC
    ACTCACAGCT TGTCTCCCCG ATCCCCTACA CCCAGCTACC GTTCCACCCC CGACTTCCCA
    TCCGGCACTA ACTCCTCCCA GAGCAGCTCC CCAAGCTCCA GCGCTCCCAA CTCCCCAGCA
    GGGTCAGGAC ACGTCCGGCC CAGCACGCTC CATGGTCTGG CGCCCAAACT CAGCGGGCAG
    CGCTACCGGT CAGGAAGGCG GAAGTCAGCT GGCAGCATCC CCCTGTCTCC GCTGGCCCGG
    ACACCCTCTC CGACCCCACA GCCCACCTCC CCGCAGCGGT CCCCATCCCC TCTGCTGGGG
    CACTCACTTG GCAATTCCAA GATGGCTCAA GCCTTTCCCA GCAAAATGCA CTCCCCACCC
    ACCATCGTCA GGCACGTCGT GAGGCCCAAG AGTGCAGAAC CCCCTCGGTC CCCGCTGCTC
    AAGCGCGTGC AGTCGGAGGA GAAGCTGTCT CCCTCCTATG GAGGCGACAA GAAGCACCTG
    TGCTCCCGCA AGCACAGCCT TGAGGTCACC CAGGAGGAGG TGCCGCGGGA GCAGCAACAG
    CGGGAGGTGA CCTTGCAGAG CCTGGAGGAG AACGTGTGCG ACGCGCCTGC CCTCAGCCGG
    GCCAGGCCCG TGGAGCAGGG CTGCCTGAAA CGCCCCGTGT CCCGGAAGCT GGGCCGCCAG
    GAGTCTGTGG ACGAGCTGGA CCGAGAGAAG CTAAAGGCCA AGGTGGTGGT GAAGAAACCT
    GACGGGCTCT CGGAAAAACA GGAATCCCGC CCGAAGCCCC ACGGACTCGG CAGTGACTTG
    GAAAACCTCT GTGCTTTTAG GCTCGAAGAG AGAGACAAGA AAATCTATCC AAAAGCTTCA
    GAGAGGTCAA ATCATTTTGA AAGCAAAGCA GCCGTGCAGG AGGCCCAGCC CCTGTGCAGC
    CTTTTGAAAG ATGCTCTCCA CAAGCAGGCC AATGTGCGGG CCAGCGAGGG TGTCGCCTCT
    GAGGGGGCAA CGGCAGCTGG GGACCACGGC CAGGGCACCT GTGACTTCAA ACGAGCCTCA
    ACGCACAGCA CCCTCCAAGA CGGCCTCTGT CACACCACCC ACAGGTCCAC CTCCGGGAAG
    GGGGACACCG CAGAGAAGGC CCCCCAGGCC AAGGAGGGTC TGCGGTGTGA GAAGTTAGAC
    GGCAAGTTGG CCAACATCGA TTACCTCCGA AAGAAGATGT CCCTTGAAGA CAAAGACGAG
    AGCCTCTGTT CTGTGCTCAA GCCCAAGGTG ACAGCTGGTG CCCAGGACTG CCTGCCTGGG
    AGCCTGGGCC GGCCCGTGGG AGGGCAGCAG GAGGCCCCGC CAGCCTCCGA GGCCCGCACA
    CCGTTCATCG GCAGCGCCCA CGCGGCTCAG CTCAGCGCCG TCTCCTTTGT CCCTCTCAAG
    GCCCTTTCTG GCCGGGTGGA CAGTGGAGCG GAGAAGCCGG GCTTGGCTGC CCCAGAGTCG
    CCTGTCCGGA AAAGCCCCTC CGAGTATAAG CTGGAAGGGC GGTCTGTGTC GTGCCTGAAG
    CCCATTGAAG GCACCTTGGA CATCGCTCTC CTGTCGGGAC CTCAGGCCTC CAAGACAGAG
    CTGCCTTCCC CGGAGCCTGC CCAGAGCCCC AGCCCAGGCA GTGATGTGGA GTCCCCCATG
    CCCCTCGCTC TCCCCAGCAG CACAGGGAGG AAGGCTGACA CTGCGGGTCA GAGAGAGTCC
    TCCCCTGCCG GCTTCAAGGT GAACAAATCC TACCTGCTTG AGCCCCGGTT CCCACCGGCC
    AGCCGGGGTC TGCAGAGTTC ACCGGTGGTT GCCCCGCCTG ACCCAGAGCT GAAGCTGGAC
    AAGAAGGTCT CCCAGGCAGC CCGTGCCCCA GCGACCATCA TGGAGAGCCA TCCCCAGCGG
    GGAGAGGGCC ACCCCAGCCA ACACCAAGAC CACAGCCCCG ACGTTAAGCT CCTTCCCTTC
    CCAGGGCAGA ACCTCCAAGG TGCCAACACG TCTAGATCCC GCAGCCCACT CTTGCCTGAA
    AGTGTCCCCC CTAGAGAGAA GCCGAGTGGG AGAGAGTTGT CCGAAAGGGG CCCTTCCATA
    GTCGGGAGCG AGTGGTCAGC TGTGAGGCCC GACATCCGCA GAGACCCCTC CAAGGAGCTG
    TGTCCCCCAG AAGCTGCTAA ACCCAGCGAC AACTCCCAAA CCCCCCCTGT GGTGGGGAGG
    ACCCGCCTGG ATGTCTCCAC CCAGACCCAG GCCTTGGAGA AAGCATGCGG GCCTTGCGGG
    AGAGCAAAGC CCAGAGACGG CCGAGAGGAG GTGAGGCTGC CGGCCAGAGA GGACTCCTCT
    TTGCGCTCTG CGGGGGCTCC TTGTGAGAGA GAGCTGGGCG CAGAGAGAGG CCTGGAATCC
    AAGATGGAAC CTGCTTCTAC CAGGTGGTCT CTGGAGCCGC CCAGGCCAGA GAGTGAGAAG
    AGTGGAAGGC CTTCTGGTTT CCAGTCTGTG CAGAAAGACG GTCCCAAGGA ACCGGAGAGG
    AAAGAACAAC CTCTGCAAAA GTACCTCACC AGTTCTCAGC CGGGTCCCAA GGAACCCGAG
    AGGAAAGAAC AGCCCCTGCA AAAGCACCTC CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CCCGAGAGGA AAGAACAGCC CCTGCAAAAG CACGTCACCA GTAGTTCTCA GCCGGGTCCC
    AAGGAACCGG AGAGGAAAGA ACAGCCTCTG CAAAAGCACC TCCCCAGTAG TTCTCAGCCG
    GGTCCCAAGG AACCGGAGAG GAAAGAACAG CCCCTGCAAA AGCACGTCAC CAGTAGTTCT
    CAGCTGGGTC CCAAGGAACC CGAGAGGAAA GAACAGCCTC TGCAAAAGCA CCTCCCCAGT
    AGTTCTCAGC CGGGTCCCAA GGAACTGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACCTC
    ACCAGTAGCT CTCAGCTGGG TCCCAAGGAA CCAGAAATGA AAGAACAGCC CCCACAAAAG
    CACGTCACCA GTAGTTCTCA ACTGTGTCCC AAGGAACTGG AAAGGAAAGA ACAACCTCCA
    CAAAAGCACC TCACTATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAG AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAACC GGAAAGGAAA
    GAACAGTGTT TGCAGAAGCA CTTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCAGAA
    ACGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA
    CCGGAAAGGA AAGAACAGCT TCTGCAAGAG CACCTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAACCGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACC TCACCAGTAG TTCTCAGCTG
    GGTCCCAAGG AACCAGAAAT GAAAGAACAG CCTCTGCAAA AGCACCTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGCCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCAGTAGCT GTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGCCCC AAGGAGCCAG AAAGGAAAGA ACAGCCTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC AGAAAGGAAA
    GAACAGCTTC TGCAAAAGCA CCTCACCATT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCTTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAAGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACC TCACCATTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA GAACAGCTTC TGCAAAAGCA CCTCACCATT
    AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCGG AAAGGAAAGA ACAGTATTTG
    CAGAAGCACC TCACCAGTAG TTCTCAGCTG GGTCCCAAGG AACCAGAAAC GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CAGTAGTTCT CAGGTGGGTC CCAAGGATCC GGAAAGGAAA
    GAACAGCCCC CACAAAAGCA CCTCACCAGT AAGTCTCAGC CGGGTCCCAA GGAACCGGAA
    AGGAAAGAAC AGTGTTTGCA AAAGCACCTC ACCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CTGGAAAGGA AAGAACAACC TCCACTAAAG CATGTCACCA GTAGCTCTCA GCTGGGTCCC
    AGGGAACCAG AAAGGAAAGA ACAGCCCCCA CAAAAGCACC TCACCAGTAG CTGTCAGCCC
    CCACCCCCTA CCAAAGAGCT CCCTGGCCTG GCTTGTCAGC ACTGCATTCT GCCCAGCCAC
    CCTGCTGCCT CTCTGCTGGG GAGCAAGCCC TGTGCTATAG ACTCCAGCTT CCAGGATGCT
    CTCAGGCCCA CTGCTGTGCG CAGTGACAGC AGCAGCCACA AGCCCAGGTC TGACCCCGAC
    CTGGGCCCTC CAAAGTCTAA GTTCCCAGAC CGGCCCCTCT CCTCCCAGAA GCCGAGCGCT
    GAGGCTGCAG TGAGCAAAGA GCCTGGCGCT CCGACCCTCG GCAGAGCTGG GGTGGGGAGT
    GGTAAGGGTG TGTCAGAGGA GTTCCCTGTC CTTCCAGATC CCCAGAACAC AGCCCGTGAA
    GTGATTGGCC AGGGAGCCAG TGGGTACTCA GGCCCCCTGC CCATGGAAGG GGGTCCTCTA
    GACACAAAGC TGAGACCCAC CAGCGCTCGG TGTCCCCCAG AGGCACTGGA GATGCTTGGA
    CATCCACCAG GGCGAGGGCT CCTAGGGGCC AGTGAGTCAG TGGACCAGAA ACTCCCCACC
    TTCAGGGAAA GGCAAAACCT GTCTCCAAAG GCCCCCACAC CATCCACTGT GAAAGATTGT
    CCCCTTCCGT GCAAACAGAC AGACAGGAGC CCGAGTCCTC TGGCCACCAG CACCAACGCT
    GGCAAGTCTG AAGGCAAGAA ATGCACTGAA GCACTTTACA CCCCTGCGGA TGGTGAGAAG
    CTGGGGGCCG GCTTGTCCTC GGTGCACAGC GAGGCTGGGC CTAAGGGCAC AGAGAAGCCG
    GCAGCAGCCG CTGGGAAGGG CTGGCCAGAG GCCAAGGGGA AAGGACTCAG TCCCCAGAAG
    CCACTGACCG AGGCCAGCAA GCCCAGCGGC ATGAAGCGGT CACCTTCAGC CACGGTGCAG
    AGCTCTCTCC GCTGTGCCAC CCTCCCAGAG AAGTCTCTGA GCTACTCCTG TGGCTTTCCC
    GAGGCCAGAC CAGCAGTTCC AGAGACTTCT ACCACCTCCA GCGACACCTC CTCTGCCAAG
    GCTGCTGGGC CTGCAGCAGA ATCCCCAGCC TCCAGCAGCA GGGACCAGCA GAAACCCCTG
    CCTGGTGGGG ATGGCAGAAC CCAAATGACC AAGAGCGACT CCTTGCCATG CTTCCGCAGC
    TCAGCCATCA CTCTGGAGTC CCAGCACCCC AGCCCTGGCC TGGTGGTGGT CACCAGCCAC
    CGGGACAGGG CCCTCTCAGT AACCGCCACC GTGGGAGAAA CCAAAGGGAA AGAGCCAGCC
    CCCACCCAGC CAGCTCAGAC CAGGAACCAG AACGTGAGCA GAGAGGCGGC CAAGCCGTCC
    CCCGCTCCAA GCACTGAGCG CCCCATCGTT CTTCCTTCTG AGAAGGACAC TGGGCTGCGA
    CAGAGGCGGG GGAAAGAGAG TTTGCGCGGC AGCCCCCACA AAAAGGCCTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837218.1
    CDS39..9452
    Translation

    Target ORF information:

    RefSeq Version XM_024981450.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTTCCCAGA 
    AGAGGAAGTC TTTGCCGAAC CAGCAACCGG AAAAGCTTGA TAGGCAATGG ACCATCCCCA
    GCATTGCCTC GGCCCCACTC ACCTCTTTCT GCTCATGCAG GAAATAGCCC TCAAGATAGT
    CCAAGAAATT TCTCCCCCAG TGCCTCTGCT CATTTTTCAT TTGCACGGAG GACTGATGGA
    CGCCGCTGGT CCTTGGCTTC TCTCCCCTCC TCTGGCTATG GGACAAACAC TCCCAGCTCT
    ACGGTCTCTT CATCCTGTTC CTCCCAGGAG AAGTTGCATC AGTTACCGTA TCAACCGACA
    CCAGATGAAT TACACTTCTT ATCAAAACAT TTTTGTACCA CTGAAAGCAT CACCACAGAG
    AACAGATGCA GAAACACACC CATGCGCCCC CGTTCCCGAA GTTTGAGCCC TGGACGTTCT
    CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA
    AAGGCTACAG CTCAGATGGA AGAGCGCCTA AAGGAGATCA TCACCAGCTA CTCTCCAGAC
    CACGTTCTCC CCCTTGCAGA TGGAGTGCTC AGCTTCACTC ACCATCAGAT TATCGAACTG
    GCTCGCGATT GTTTGGATAA ATCCCACCAG GGTCTCATCA CCTCACGATA CTTCCTTGAA
    TTACAGCACA AATTAGATAA GTTACTACAG GAGGCTCACA ATCGTTCAGA AAGTGGAGAG
    CTGGCATTTA TTAAACAACT AGTTCGAAAG ATCCTGATTG TTATTGCCCG CCCTGCTCGC
    TTATTAGAGT GCCTGGAATT TGATCCTGAA GAATTTTACT ACCTATTGGA AGCAGCAGAA
    GGCCATGCCA AAGAAGGACA GGGTATTAAA ACTGACATTC CCAGGTACAT CATTAGCCAG
    CTTGGCCTCA ATAAAGATCC TCTGGAAGAA ATGGCTCAAC TGGGAAACTA TGATAGTGGG
    ACAGCAGAAA CACCAGAAAC AGATGAATCA GTGAGTAGCT CTAATGCTTC CCTGAAACTT
    CGAAGGAAGC CTCGGGAAAG TGATTTTGAA ACGATTAAAT TGATCAGCAA TGGAGCCTAC
    GGGGCAGTCT ATTTCGTTCG GCATAAGGAA TCCCGGCAGA GGTTTGCCAT GAAGAAGATT
    AACAAACAGA ACCTCATCCT CCGAAACCAG ATCCAGCAGG CCTTTGTGGA ACGGGATATC
    CTGACTTTTG CAGAAAACCC TTTTGTCGTC AGCATGTACT GCTCCTTTGA GACCAGGCGC
    CACTTGTGCA TGGTCATGGA GTATGTGGAA GGGGGTGACT GTGCCACGTT AATGAAGAAC
    ATGGGTCCTC TCCCCGTAGA TATGGCCAGG ATGTACTTTG CTGAGACGGT CCTGGCCTTG
    GAATATCTAC ATAATTATGG AATTGTACAT AGGGATTTGA AACCAGACAA TTTGCTGGTC
    ACCTCTATGG GGCACATAAA GCTGACCGAT TTTGGATTAT CCAAGGTGGG ACTCATGAGC
    ATGACTACCA ATCTTTATGA GGGTCATATT GAGAAGGATG CCCGAGAGTT CCTGGACAAG
    CAGGTCTGTG GCACACCTGA ATACATTGCA CCAGAAGTGA TTCTGAGGCA GGGCTACGGG
    AAGCCAGTGG ACTGGTGGGC CATGGGGATT ATCCTCTATG AATTTCTGGT TGGATGCGTG
    CCATTCTTTG GGGACACTCC AGAAGAGCTA TTTGGACAAG TCATCAGTGA TGAGATCAAC
    TGGCCTGAAA AGGATGAGGC TCCACCCCCT GATGCCCAGG ATCTGATTAC CTTGCTCCTC
    AGGCAGAATC CCCTGGAGAG ACTGGGAACA GGTGGTGCAT ATGAAGTCAA ACAGCATCGA
    TTCTTCCGTT GCTTAGACTG GAACAGTTTG CTGAGACAGA AGGCAGAATT CATTCCCCAA
    CTGGAATCTG AAGACGACAC AAGTTATTTT GATACTCGGT CAGAGAAGTA CCATCATATG
    GAGACTGAGG AAGAAGATGA CACGAACGAT GAAGACTTTA ATGTGGAAAT AAGGCAGTTT
    TCCTCATGTT CACATAGGTT TTCTAAGGTC TTCAGCAGTA TGGATCGAGG TACTCAGAAT
    TCAGGAGAAG AGAAGGAAGA CCCTGGGGAC AAAACTAAAA GCACAGCTTT GCCGTCCACG
    GAAACACTAA GCTGGAGTTC AGAGTATTCT GAAATGCAAC AGTTATCCAC ATCCAACTCT
    TCAGACACTG AAAGCAACAG ACATAGACGC GGTGCTGGGC TGCTTCCCAA GCTGGCTGTT
    TCAGCAGAGG CAGAACACGA CGGGGCCGCT CCCCGCCCCA GAGAGCTGCA CGAGGAGCCG
    GAAAAGCCAG CCCTGCCTGC TGCAGAGAAT GCTCAGGAGG AGCCCGAGGT CACTACCCCG
    GCCAGCACCA TCAGCAGCTC CACTCTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG
    ATAAATGGAC GGAGCGAGTG TGTGGACAGT ACAGACAATG CCTCCAAGCC CTCCAATGAG
    CCCGCCTCTC ACATGGCTCG GCAGCGCTTA GAAAGCACAG AGAAACAGAA AGCCTCGGGG
    AAAGTCACAA AGTCCCTTTC TGCCAGTGCC CTGTCCCTCA TGATCCCAGG AGATATGTTT
    GCTGTCTCTC CGCTGGGAAG TCCGATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCC
    TCAAGGGATT CCTCTCCCAG CCGAGATTCT TCAGGAGCTT CTGCCAGTCC ACACCAGCCC
    ATTGTGATCC ACAGTTTGGG GAAGAACTAT GGTTTCACCA TTCGAGCCAT CCGGGTGTAC
    GTGGGAGACA GCGACATTTA CACAGTGCAT CATATCGTTT GGAATGTAGA AGAAGGAAGT
    CCAGCTTGCC AGGCAGGACT GAAAGCTGGG GATCTGATCA CACACATCAA TGGAGAACCG
    GTGCACGGAC TTGTCCACAC AGAAGTTATA GAGCTCCTGC TAAAGAGTGG AAATAAGGTG
    TCAATCACGA CCACCCCATT TGAAAACACA TCAATCAAAA CGGGACCGGC CAGGAGAAAC
    AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCTA AGAAGAAGGA AAGTCTGGAA
    AGGAGGAGAT CCCTTTTCAA AAAACTAGCT AAGCAGCCTT CTCCTTTACT CCACACCAGC
    CGAAGTTTCT CCTGCTTGAA CCGATCCCTG TCGTCAGGGG AGAGCCTCCC AGGGTCCCCC
    ACTCACAGCT TGTCTCCCCG ATCCCCTACA CCCAGCTACC GTTCCACCCC CGACTTCCCA
    TCCGGCACTA ACTCCTCCCA GAGCAGCTCC CCAAGCTCCA GCGCTCCCAA CTCCCCAGCA
    GGGTCAGGAC ACGTCCGGCC CAGCACGCTC CATGGTCTGG CGCCCAAACT CAGCGGGCAG
    CGCTACCGGT CAGGAAGGCG GAAGTCAGCT GGCAGCATCC CCCTGTCTCC GCTGGCCCGG
    ACACCCTCTC CGACCCCACA GCCCACCTCC CCGCAGCGGT CCCCATCCCC TCTGCTGGGG
    CACTCACTTG GCAATTCCAA GATGGCTCAA GCCTTTCCCA GCAAAATGCA CTCCCCACCC
    ACCATCGTCA GGCACGTCGT GAGGCCCAAG AGTGCAGAAC CCCCTCGGTC CCCGCTGCTC
    AAGCGCGTGC AGTCGGAGGA GAAGCTGTCT CCCTCCTATG GAGGCGACAA GAAGCACCTG
    TGCTCCCGCA AGCACAGCCT TGAGGTCACC CAGGAGGAGG TGCCGCGGGA GCAGCAACAG
    CGGGAGGTGA CCTTGCAGAG CCTGGAGGAG AACGTGTGCG ACGCGCCTGC CCTCAGCCGG
    GCCAGGCCCG TGGAGCAGGG CTGCCTGAAA CGCCCCGTGT CCCGGAAGCT GGGCCGCCAG
    GAGTCTGTGG ACGAGCTGGA CCGAGAGAAG CTAAAGGCCA AGGTGGTGGT GAAGAAACCT
    GACGGGCTCT CGGAAAAACA GGAATCCCGC CCGAAGCCCC ACGGACTCGG CAGTGACTTG
    GAAAACCTCT GTGCTTTTAG GCTCGAAGAG AGAGACAAGA AAATCTATCC AAAAGCTTCA
    GAGAGGTCAA ATCATTTTGA AAGCAAAGCA GCCGTGCAGG AGGCCCAGCC CCTGTGCAGC
    CTTTTGAAAG ATGCTCTCCA CAAGCAGGCC AATGTGCGGG CCAGCGAGGG TGTCGCCTCT
    GAGGGGGCAA CGGCAGCTGG GGACCACGGC CAGGGCACCT GTGACTTCAA ACGAGCCTCA
    ACGCACAGCA CCCTCCAAGA CGGCCTCTGT CACACCACCC ACAGGTCCAC CTCCGGGAAG
    GGGGACACCG CAGAGAAGGC CCCCCAGGCC AAGGAGGGTC TGCGGTGTGA GAAGTTAGAC
    GGCAAGTTGG CCAACATCGA TTACCTCCGA AAGAAGATGT CCCTTGAAGA CAAAGACGAG
    AGCCTCTGTT CTGTGCTCAA GCCCAAGGTG ACAGCTGGTG CCCAGGACTG CCTGCCTGGG
    AGCCTGGGCC GGCCCGTGGG AGGGCAGCAG GAGGCCCCGC CAGCCTCCGA GGCCCGCACA
    CCGTTCATCG GCAGCGCCCA CGCGGCTCAG CTCAGCGCCG TCTCCTTTGT CCCTCTCAAG
    GCCCTTTCTG GCCGGGTGGA CAGTGGAGCG GAGAAGCCGG GCTTGGCTGC CCCAGAGTCG
    CCTGTCCGGA AAAGCCCCTC CGAGTATAAG CTGGAAGGGC GGTCTGTGTC GTGCCTGAAG
    CCCATTGAAG GCACCTTGGA CATCGCTCTC CTGTCGGGAC CTCAGGCCTC CAAGACAGAG
    CTGCCTTCCC CGGAGCCTGC CCAGAGCCCC AGCCCAGGCA GTGATGTGGA GTCCCCCATG
    CCCCTCGCTC TCCCCAGCAG CACAGGGAGG AAGGCTGACA CTGCGGGTCA GAGAGAGTCC
    TCCCCTGCCG GCTTCAAGGT GAACAAATCC TACCTGCTTG AGCCCCGGTT CCCACCGGCC
    AGCCGGGGTC TGCAGAGTTC ACCGGTGGTT GCCCCGCCTG ACCCAGAGCT GAAGCTGGAC
    AAGAAGGTCT CCCAGGCAGC CCGTGCCCCA GCGACCATCA TGGAGAGCCA TCCCCAGCGG
    GGAGAGGGCC ACCCCAGCCA ACACCAAGAC CACAGCCCCG ACGTTAAGCT CCTTCCCTTC
    CCAGGGCAGA ACCTCCAAGG TGCCAACACG TCTAGATCCC GCAGCCCACT CTTGCCTGAA
    AGTGTCCCCC CTAGAGAGAA GCCGAGTGGG AGAGAGTTGT CCGAAAGGGG CCCTTCCATA
    GTCGGGAGCG AGTGGTCAGC TGTGAGGCCC GACATCCGCA GAGACCCCTC CAAGGAGCTG
    TGTCCCCCAG AAGCTGCTAA ACCCAGCGAC AACTCCCAAA CCCCCCCTGT GGTGGGGAGG
    ACCCGCCTGG ATGTCTCCAC CCAGACCCAG GCCTTGGAGA AAGCATGCGG GCCTTGCGGG
    AGAGCAAAGC CCAGAGACGG CCGAGAGGAG GTGAGGCTGC CGGCCAGAGA GGACTCCTCT
    TTGCGCTCTG CGGGGGCTCC TTGTGAGAGA GAGCTGGGCG CAGAGAGAGG CCTGGAATCC
    AAGATGGAAC CTGCTTCTAC CAGGTGGTCT CTGGAGCCGC CCAGGCCAGA GAGTGAGAAG
    AGTGGAAGGC CTTCTGGTTT CCAGTCTGTG CAGAAAGACG GTCCCAAGGA ACCGGAGAGG
    AAAGAACAAC CTCTGCAAAA GTACCTCACC AGTTCTCAGC CGGGTCCCAA GGAACCCGAG
    AGGAAAGAAC AGCCCCTGCA AAAGCACCTC CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CCCGAGAGGA AAGAACAGCC CCTGCAAAAG CACGTCACCA GTAGTTCTCA GCCGGGTCCC
    AAGGAACCGG AGAGGAAAGA ACAGCCTCTG CAAAAGCACC TCCCCAGTAG TTCTCAGCCG
    GGTCCCAAGG AACCGGAGAG GAAAGAACAG CCCCTGCAAA AGCACGTCAC CAGTAGTTCT
    CAGCTGGGTC CCAAGGAACC CGAGAGGAAA GAACAGCCTC TGCAAAAGCA CCTCCCCAGT
    AGTTCTCAGC CGGGTCCCAA GGAACTGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACCTC
    ACCAGTAGCT CTCAGCTGGG TCCCAAGGAA CCAGAAATGA AAGAACAGCC CCCACAAAAG
    CACGTCACCA GTAGTTCTCA ACTGTGTCCC AAGGAACTGG AAAGGAAAGA ACAACCTCCA
    CAAAAGCACC TCACTATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAG AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAACC GGAAAGGAAA
    GAACAGTGTT TGCAGAAGCA CTTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCAGAA
    ACGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA
    CCGGAAAGGA AAGAACAGCT TCTGCAAGAG CACCTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAACCGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACC TCACCAGTAG TTCTCAGCTG
    GGTCCCAAGG AACCAGAAAT GAAAGAACAG CCTCTGCAAA AGCACCTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGCCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCAGTAGCT GTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGCCCC AAGGAGCCAG AAAGGAAAGA ACAGCCTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC AGAAAGGAAA
    GAACAGCTTC TGCAAAAGCA CCTCACCATT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCTTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAAGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACC TCACCATTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA GAACAGCTTC TGCAAAAGCA CCTCACCATT
    AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCGG AAAGGAAAGA ACAGTATTTG
    CAGAAGCACC TCACCAGTAG TTCTCAGCTG GGTCCCAAGG AACCAGAAAC GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CAGTAGTTCT CAGGTGGGTC CCAAGGATCC GGAAAGGAAA
    GAACAGCCCC CACAAAAGCA CCTCACCAGT AAGTCTCAGC CGGGTCCCAA GGAACCGGAA
    AGGAAAGAAC AGTGTTTGCA AAAGCACCTC ACCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CTGGAAAGGA AAGAACAACC TCCACTAAAG CATGTCACCA GTAGCTCTCA GCTGGGTCCC
    AGGGAACCAG AAAGGAAAGA ACAGCCCCCA CAAAAGCACC TCACCAGTAG CTGTCAGCCC
    CCACCCCCTA CCAAAGAGCT CCCTGGCCTG GCTTGTCAGC ACTGCATTCT GCCCAGCCAC
    CCTGCTGCCT CTCTGCTGGG GAGCAAGCCC TGTGCTATAG ACTCCAGCTT CCAGGATGCT
    CTCAGGCCCA CTGCTGTGCG CAGTGACAGC AGCAGCCACA AGCCCAGGTC TGACCCCGAC
    CTGGGCCCTC CAAAGTCTAA GTTCCCAGAC CGGCCCCTCT CCTCCCAGAA GCCGAGCGCT
    GAGGCTGCAG TGAGCAAAGA GCCTGGCGCT CCGACCCTCG GCAGAGCTGG GGTGGGGAGT
    GGTAAGGGTG TGTCAGAGGA GTTCCCTGTC CTTCCAGATC CCCAGAACAC AGCCCGTGAA
    GTGATTGGCC AGGGAGCCAG TGGGTACTCA GGCCCCCTGC CCATGGAAGG GGGTCCTCTA
    GACACAAAGC TGAGACCCAC CAGCGCTCGG TGTCCCCCAG AGGCACTGGA GATGCTTGGA
    CATCCACCAG GGCGAGGGCT CCTAGGGGCC AGTGAGTCAG TGGACCAGAA ACTCCCCACC
    TTCAGGGAAA GGCAAAACCT GTCTCCAAAG GCCCCCACAC CATCCACTGT GAAAGATTGT
    CCCCTTCCGT GCAAACAGAC AGACAGGAGC CCGAGTCCTC TGGCCACCAG CACCAACGCT
    GGCAAGTCTG AAGGCAAGAA ATGCACTGAA GCACTTTACA CCCCTGCGGA TGGTGAGAAG
    CTGGGGGCCG GCTTGTCCTC GGTGCACAGC GAGGCTGGGC CTAAGGGCAC AGAGAAGCCG
    GCAGCAGCCG CTGGGAAGGG CTGGCCAGAG GCCAAGGGGA AAGGACTCAG TCCCCAGAAG
    CCACTGACCG AGGCCAGCAA GCCCAGCGGC ATGAAGCGGT CACCTTCAGC CACGGTGCAG
    AGCTCTCTCC GCTGTGCCAC CCTCCCAGAG AAGTCTCTGA GCTACTCCTG TGGCTTTCCC
    GAGGCCAGAC CAGCAGTTCC AGAGACTTCT ACCACCTCCA GCGACACCTC CTCTGCCAAG
    GCTGCTGGGC CTGCAGCAGA ATCCCCAGCC TCCAGCAGCA GGGACCAGCA GAAACCCCTG
    CCTGGTGGGG ATGGCAGAAC CCAAATGACC AAGAGCGACT CCTTGCCATG CTTCCGCAGC
    TCAGCCATCA CTCTGGAGTC CCAGCACCCC AGCCCTGGCC TGGTGGTGGT CACCAGCCAC
    CGGGACAGGG CCCTCTCAGT AACCGCCACC GTGGGAGAAA CCAAAGGGAA AGAGCCAGCC
    CCCACCCAGC CAGCTCAGAC CAGGAACCAG AACGTGAGCA GAGAGGCGGC CAAGCCGTCC
    CCCGCTCCAA GCACTGAGCG CCCCATCGTT CTTCCTTCTG AGAAGGACAC TGGGCTGCGA
    CAGAGGCGGG GGAAAGAGAG TTTGCGCGGC AGCCCCCACA AAAAGGCCTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228117
    Clone ID Related Accession (Same CDS sequence) XM_024981451.1
    Accession Version XM_024981451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGGCCCAAC ATTTATGGAT TTTGTTGGGA AGTTCCAGTT GCCGAACCAG CAACCGGAAA 
    AGCTTGATAG GCAATGGACC ATCCCCAGCA TTGCCTCGGC CCCACTCACC TCTTTCTGCT
    CATGCAGGAA ATAGCCCTCA AGATAGTCCA AGAAATTTCT CCCCCAGTGC CTCTGCTCAT
    TTTTCATTTG CACGGAGGAC TGATGGACGC CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT
    GGCTATGGGA CAAACACTCC CAGCTCTACG GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG
    TTGCATCAGT TACCGTATCA ACCGACACCA GATGAATTAC ACTTCTTATC AAAACATTTT
    TGTACCACTG AAAGCATCAC CACAGAGAAC AGATGCAGAA ACACACCCAT GCGCCCCCGT
    TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC GCCTGCTGTG ACCATGAAAT AATTATGATG
    AACCATGTCT ACAAAGAAAG GTTCCCAAAG GCTACAGCTC AGATGGAAGA GCGCCTAAAG
    GAGATCATCA CCAGCTACTC TCCAGACCAC GTTCTCCCCC TTGCAGATGG AGTGCTCAGC
    TTCACTCACC ATCAGATTAT CGAACTGGCT CGCGATTGTT TGGATAAATC CCACCAGGGT
    CTCATCACCT CACGATACTT CCTTGAATTA CAGCACAAAT TAGATAAGTT ACTACAGGAG
    GCTCACAATC GTTCAGAAAG TGGAGAGCTG GCATTTATTA AACAACTAGT TCGAAAGATC
    CTGATTGTTA TTGCCCGCCC TGCTCGCTTA TTAGAGTGCC TGGAATTTGA TCCTGAAGAA
    TTTTACTACC TATTGGAAGC AGCAGAAGGC CATGCCAAAG AAGGACAGGG TATTAAAACT
    GACATTCCCA GGTACATCAT TAGCCAGCTT GGCCTCAATA AAGATCCTCT GGAAGAAATG
    GCTCAACTGG GAAACTATGA TAGTGGGACA GCAGAAACAC CAGAAACAGA TGAATCAGTG
    AGTAGCTCTA ATGCTTCCCT GAAACTTCGA AGGAAGCCTC GGGAAAGTGA TTTTGAAACG
    ATTAAATTGA TCAGCAATGG AGCCTACGGG GCAGTCTATT TCGTTCGGCA TAAGGAATCC
    CGGCAGAGGT TTGCCATGAA GAAGATTAAC AAACAGAACC TCATCCTCCG AAACCAGATC
    CAGCAGGCCT TTGTGGAACG GGATATCCTG ACTTTTGCAG AAAACCCTTT TGTCGTCAGC
    ATGTACTGCT CCTTTGAGAC CAGGCGCCAC TTGTGCATGG TCATGGAGTA TGTGGAAGGG
    GGTGACTGTG CCACGTTAAT GAAGAACATG GGTCCTCTCC CCGTAGATAT GGCCAGGATG
    TACTTTGCTG AGACGGTCCT GGCCTTGGAA TATCTACATA ATTATGGAAT TGTACATAGG
    GATTTGAAAC CAGACAATTT GCTGGTCACC TCTATGGGGC ACATAAAGCT GACCGATTTT
    GGATTATCCA AGGTGGGACT CATGAGCATG ACTACCAATC TTTATGAGGG TCATATTGAG
    AAGGATGCCC GAGAGTTCCT GGACAAGCAG GTCTGTGGCA CACCTGAATA CATTGCACCA
    GAAGTGATTC TGAGGCAGGG CTACGGGAAG CCAGTGGACT GGTGGGCCAT GGGGATTATC
    CTCTATGAAT TTCTGGTTGG ATGCGTGCCA TTCTTTGGGG ACACTCCAGA AGAGCTATTT
    GGACAAGTCA TCAGTGATGA GATCAACTGG CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT
    GCCCAGGATC TGATTACCTT GCTCCTCAGG CAGAATCCCC TGGAGAGACT GGGAACAGGT
    GGTGCATATG AAGTCAAACA GCATCGATTC TTCCGTTGCT TAGACTGGAA CAGTTTGCTG
    AGACAGAAGG CAGAATTCAT TCCCCAACTG GAATCTGAAG ACGACACAAG TTATTTTGAT
    ACTCGGTCAG AGAAGTACCA TCATATGGAG ACTGAGGAAG AAGATGACAC GAACGATGAA
    GACTTTAATG TGGAAATAAG GCAGTTTTCC TCATGTTCAC ATAGGTTTTC TAAGGTCTTC
    AGCAGTATGG ATCGAGGTAC TCAGAATTCA GGAGAAGAGA AGGAAGACCC TGGGGACAAA
    ACTAAAAGCA CAGCTTTGCC GTCCACGGAA ACACTAAGCT GGAGTTCAGA GTATTCTGAA
    ATGCAACAGT TATCCACATC CAACTCTTCA GACACTGAAA GCAACAGACA TAGACGCGGT
    GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA GCAGAGGCAG AACACGACGG GGCCGCTCCC
    CGCCCCAGAG AGCTGCACGA GGAGCCGGAA AAGCCAGCCC TGCCTGCTGC AGAGAATGCT
    CAGGAGGAGC CCGAGGTCAC TACCCCGGCC AGCACCATCA GCAGCTCCAC TCTGTCAGTT
    GGCAGTTTTT CAGAGCACTT GGATCAGATA AATGGACGGA GCGAGTGTGT GGACAGTACA
    GACAATGCCT CCAAGCCCTC CAATGAGCCC GCCTCTCACA TGGCTCGGCA GCGCTTAGAA
    AGCACAGAGA AACAGAAAGC CTCGGGGAAA GTCACAAAGT CCCTTTCTGC CAGTGCCCTG
    TCCCTCATGA TCCCAGGAGA TATGTTTGCT GTCTCTCCGC TGGGAAGTCC GATGTCTCCC
    CATTCCCTGT CCTCGGACCC TTCTTCCTCA AGGGATTCCT CTCCCAGCCG AGATTCTTCA
    GGAGCTTCTG CCAGTCCACA CCAGCCCATT GTGATCCACA GTTTGGGGAA GAACTATGGT
    TTCACCATTC GAGCCATCCG GGTGTACGTG GGAGACAGCG ACATTTACAC AGTGCATCAT
    ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA GCTTGCCAGG CAGGACTGAA AGCTGGGGAT
    CTGATCACAC ACATCAATGG AGAACCGGTG CACGGACTTG TCCACACAGA AGTTATAGAG
    CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA ATCACGACCA CCCCATTTGA AAACACATCA
    ATCAAAACGG GACCGGCCAG GAGAAACAGC TATAAGAGCC GGATGGTGAG GCGGAGCAAG
    AAATCTAAGA AGAAGGAAAG TCTGGAAAGG AGGAGATCCC TTTTCAAAAA ACTAGCTAAG
    CAGCCTTCTC CTTTACTCCA CACCAGCCGA AGTTTCTCCT GCTTGAACCG ATCCCTGTCG
    TCAGGGGAGA GCCTCCCAGG GTCCCCCACT CACAGCTTGT CTCCCCGATC CCCTACACCC
    AGCTACCGTT CCACCCCCGA CTTCCCATCC GGCACTAACT CCTCCCAGAG CAGCTCCCCA
    AGCTCCAGCG CTCCCAACTC CCCAGCAGGG TCAGGACACG TCCGGCCCAG CACGCTCCAT
    GGTCTGGCGC CCAAACTCAG CGGGCAGCGC TACCGGTCAG GAAGGCGGAA GTCAGCTGGC
    AGCATCCCCC TGTCTCCGCT GGCCCGGACA CCCTCTCCGA CCCCACAGCC CACCTCCCCG
    CAGCGGTCCC CATCCCCTCT GCTGGGGCAC TCACTTGGCA ATTCCAAGAT GGCTCAAGCC
    TTTCCCAGCA AAATGCACTC CCCACCCACC ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT
    GCAGAACCCC CTCGGTCCCC GCTGCTCAAG CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC
    TCCTATGGAG GCGACAAGAA GCACCTGTGC TCCCGCAAGC ACAGCCTTGA GGTCACCCAG
    GAGGAGGTGC CGCGGGAGCA GCAACAGCGG GAGGTGACCT TGCAGAGCCT GGAGGAGAAC
    GTGTGCGACG CGCCTGCCCT CAGCCGGGCC AGGCCCGTGG AGCAGGGCTG CCTGAAACGC
    CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG TCTGTGGACG AGCTGGACCG AGAGAAGCTA
    AAGGCCAAGG TGGTGGTGAA GAAACCTGAC GGGCTCTCGG AAAAACAGGA ATCCCGCCCG
    AAGCCCCACG GACTCGGCAG TGACTTGGAA AACCTCTGTG CTTTTAGGCT CGAAGAGAGA
    GACAAGAAAA TCTATCCAAA AGCTTCAGAG AGGTCAAATC ATTTTGAAAG CAAAGCAGCC
    GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT TTGAAAGATG CTCTCCACAA GCAGGCCAAT
    GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG GGGGCAACGG CAGCTGGGGA CCACGGCCAG
    GGCACCTGTG ACTTCAAACG AGCCTCAACG CACAGCACCC TCCAAGACGG CCTCTGTCAC
    ACCACCCACA GGTCCACCTC CGGGAAGGGG GACACCGCAG AGAAGGCCCC CCAGGCCAAG
    GAGGGTCTGC GGTGTGAGAA GTTAGACGGC AAGTTGGCCA ACATCGATTA CCTCCGAAAG
    AAGATGTCCC TTGAAGACAA AGACGAGAGC CTCTGTTCTG TGCTCAAGCC CAAGGTGACA
    GCTGGTGCCC AGGACTGCCT GCCTGGGAGC CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG
    GCCCCGCCAG CCTCCGAGGC CCGCACACCG TTCATCGGCA GCGCCCACGC GGCTCAGCTC
    AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC CTTTCTGGCC GGGTGGACAG TGGAGCGGAG
    AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT GTCCGGAAAA GCCCCTCCGA GTATAAGCTG
    GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC ATTGAAGGCA CCTTGGACAT CGCTCTCCTG
    TCGGGACCTC AGGCCTCCAA GACAGAGCTG CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC
    CCAGGCAGTG ATGTGGAGTC CCCCATGCCC CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG
    GCTGACACTG CGGGTCAGAG AGAGTCCTCC CCTGCCGGCT TCAAGGTGAA CAAATCCTAC
    CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC CGGGGTCTGC AGAGTTCACC GGTGGTTGCC
    CCGCCTGACC CAGAGCTGAA GCTGGACAAG AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG
    ACCATCATGG AGAGCCATCC CCAGCGGGGA GAGGGCCACC CCAGCCAACA CCAAGACCAC
    AGCCCCGACG TTAAGCTCCT TCCCTTCCCA GGGCAGAACC TCCAAGGTGC CAACACGTCT
    AGATCCCGCA GCCCACTCTT GCCTGAAAGT GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA
    GAGTTGTCCG AAAGGGGCCC TTCCATAGTC GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC
    ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT CCCCCAGAAG CTGCTAAACC CAGCGACAAC
    TCCCAAACCC CCCCTGTGGT GGGGAGGACC CGCCTGGATG TCTCCACCCA GACCCAGGCC
    TTGGAGAAAG CATGCGGGCC TTGCGGGAGA GCAAAGCCCA GAGACGGCCG AGAGGAGGTG
    AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG
    CTGGGCGCAG AGAGAGGCCT GGAATCCAAG ATGGAACCTG CTTCTACCAG GTGGTCTCTG
    GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG
    AAAGACGGTC CCAAGGAACC GGAGAGGAAA GAACAACCTC TGCAAAAGTA CCTCACCAGT
    TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG AAAGAACAGC CCCTGCAAAA GCACCTCCCC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACCC GAGAGGAAAG AACAGCCCCT GCAAAAGCAC
    GTCACCAGTA GTTCTCAGCC GGGTCCCAAG GAACCGGAGA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCC CCAGTAGTTC TCAGCCGGGT CCCAAGGAAC CGGAGAGGAA AGAACAGCCC
    CTGCAAAAGC ACGTCACCAG TAGTTCTCAG CTGGGTCCCA AGGAACCCGA GAGGAAAGAA
    CAGCCTCTGC AAAAGCACCT CCCCAGTAGT TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG
    AAAGAACAGT GTTTGCAGAA GCACCTCACC AGTAGCTCTC AGCTGGGTCC CAAGGAACCA
    GAAATGAAAG AACAGCCCCC ACAAAAGCAC GTCACCAGTA GTTCTCAACT GTGTCCCAAG
    GAACTGGAAA GGAAAGAACA ACCTCCACAA AAGCACCTCA CTATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAGAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCGGA AAGGAAAGAA CAGTGTTTGC AGAAGCACTT CACCAGTAGT
    TCTCAGCTGG GTCCCAAGGA ACCAGAAACG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAACCG GAAAGGAAAG AACAGCTTCT GCAAGAGCAC
    CTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAATGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCAGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACGT CACCAGTAGC TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGCCCCAAG
    GAGCCAGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC TTCTGCAAAA GCACCTCACC
    ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAAGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAGCCGGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTATTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAACGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGTTCTCAG
    GTGGGTCCCA AGGATCCGGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAAG
    TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG AAAGAACAGT GTTTGCAAAA GCACCTCACC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACTG GAAAGGAAAG AACAACCTCC ACTAAAGCAT
    GTCACCAGTA GCTCTCAGCT GGGTCCCAGG GAACCAGAAA GGAAAGAACA GCCCCCACAA
    AAGCACCTCA CCAGTAGCTG TCAGCCCCCA CCCCCTACCA AAGAGCTCCC TGGCCTGGCT
    TGTCAGCACT GCATTCTGCC CAGCCACCCT GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT
    GCTATAGACT CCAGCTTCCA GGATGCTCTC AGGCCCACTG CTGTGCGCAG TGACAGCAGC
    AGCCACAAGC CCAGGTCTGA CCCCGACCTG GGCCCTCCAA AGTCTAAGTT CCCAGACCGG
    CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG
    ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT
    CCAGATCCCC AGAACACAGC CCGTGAAGTG ATTGGCCAGG GAGCCAGTGG GTACTCAGGC
    CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC ACAAAGCTGA GACCCACCAG CGCTCGGTGT
    CCCCCAGAGG CACTGGAGAT GCTTGGACAT CCACCAGGGC GAGGGCTCCT AGGGGCCAGT
    GAGTCAGTGG ACCAGAAACT CCCCACCTTC AGGGAAAGGC AAAACCTGTC TCCAAAGGCC
    CCCACACCAT CCACTGTGAA AGATTGTCCC CTTCCGTGCA AACAGACAGA CAGGAGCCCG
    AGTCCTCTGG CCACCAGCAC CAACGCTGGC AAGTCTGAAG GCAAGAAATG CACTGAAGCA
    CTTTACACCC CTGCGGATGG TGAGAAGCTG GGGGCCGGCT TGTCCTCGGT GCACAGCGAG
    GCTGGGCCTA AGGGCACAGA GAAGCCGGCA GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC
    AAGGGGAAAG GACTCAGTCC CCAGAAGCCA CTGACCGAGG CCAGCAAGCC CAGCGGCATG
    AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC TCTCTCCGCT GTGCCACCCT CCCAGAGAAG
    TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG GCCAGACCAG CAGTTCCAGA GACTTCTACC
    ACCTCCAGCG ACACCTCCTC TGCCAAGGCT GCTGGGCCTG CAGCAGAATC CCCAGCCTCC
    AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT GGTGGGGATG GCAGAACCCA AATGACCAAG
    AGCGACTCCT TGCCATGCTT CCGCAGCTCA GCCATCACTC TGGAGTCCCA GCACCCCAGC
    CCTGGCCTGG TGGTGGTCAC CAGCCACCGG GACAGGGCCC TCTCAGTAAC CGCCACCGTG
    GGAGAAACCA AAGGGAAAGA GCCAGCCCCC ACCCAGCCAG CTCAGACCAG GAACCAGAAC
    GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC GCTCCAAGCA CTGAGCGCCC CATCGTTCTT
    CCTTCTGAGA AGGACACTGG GCTGCGACAG AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC
    CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837219.1
    CDS34..9414
    Translation

    Target ORF information:

    RefSeq Version XM_024981451.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGGCCCAAC ATTTATGGAT TTTGTTGGGA AGTTCCAGTT GCCGAACCAG CAACCGGAAA 
    AGCTTGATAG GCAATGGACC ATCCCCAGCA TTGCCTCGGC CCCACTCACC TCTTTCTGCT
    CATGCAGGAA ATAGCCCTCA AGATAGTCCA AGAAATTTCT CCCCCAGTGC CTCTGCTCAT
    TTTTCATTTG CACGGAGGAC TGATGGACGC CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT
    GGCTATGGGA CAAACACTCC CAGCTCTACG GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG
    TTGCATCAGT TACCGTATCA ACCGACACCA GATGAATTAC ACTTCTTATC AAAACATTTT
    TGTACCACTG AAAGCATCAC CACAGAGAAC AGATGCAGAA ACACACCCAT GCGCCCCCGT
    TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC GCCTGCTGTG ACCATGAAAT AATTATGATG
    AACCATGTCT ACAAAGAAAG GTTCCCAAAG GCTACAGCTC AGATGGAAGA GCGCCTAAAG
    GAGATCATCA CCAGCTACTC TCCAGACCAC GTTCTCCCCC TTGCAGATGG AGTGCTCAGC
    TTCACTCACC ATCAGATTAT CGAACTGGCT CGCGATTGTT TGGATAAATC CCACCAGGGT
    CTCATCACCT CACGATACTT CCTTGAATTA CAGCACAAAT TAGATAAGTT ACTACAGGAG
    GCTCACAATC GTTCAGAAAG TGGAGAGCTG GCATTTATTA AACAACTAGT TCGAAAGATC
    CTGATTGTTA TTGCCCGCCC TGCTCGCTTA TTAGAGTGCC TGGAATTTGA TCCTGAAGAA
    TTTTACTACC TATTGGAAGC AGCAGAAGGC CATGCCAAAG AAGGACAGGG TATTAAAACT
    GACATTCCCA GGTACATCAT TAGCCAGCTT GGCCTCAATA AAGATCCTCT GGAAGAAATG
    GCTCAACTGG GAAACTATGA TAGTGGGACA GCAGAAACAC CAGAAACAGA TGAATCAGTG
    AGTAGCTCTA ATGCTTCCCT GAAACTTCGA AGGAAGCCTC GGGAAAGTGA TTTTGAAACG
    ATTAAATTGA TCAGCAATGG AGCCTACGGG GCAGTCTATT TCGTTCGGCA TAAGGAATCC
    CGGCAGAGGT TTGCCATGAA GAAGATTAAC AAACAGAACC TCATCCTCCG AAACCAGATC
    CAGCAGGCCT TTGTGGAACG GGATATCCTG ACTTTTGCAG AAAACCCTTT TGTCGTCAGC
    ATGTACTGCT CCTTTGAGAC CAGGCGCCAC TTGTGCATGG TCATGGAGTA TGTGGAAGGG
    GGTGACTGTG CCACGTTAAT GAAGAACATG GGTCCTCTCC CCGTAGATAT GGCCAGGATG
    TACTTTGCTG AGACGGTCCT GGCCTTGGAA TATCTACATA ATTATGGAAT TGTACATAGG
    GATTTGAAAC CAGACAATTT GCTGGTCACC TCTATGGGGC ACATAAAGCT GACCGATTTT
    GGATTATCCA AGGTGGGACT CATGAGCATG ACTACCAATC TTTATGAGGG TCATATTGAG
    AAGGATGCCC GAGAGTTCCT GGACAAGCAG GTCTGTGGCA CACCTGAATA CATTGCACCA
    GAAGTGATTC TGAGGCAGGG CTACGGGAAG CCAGTGGACT GGTGGGCCAT GGGGATTATC
    CTCTATGAAT TTCTGGTTGG ATGCGTGCCA TTCTTTGGGG ACACTCCAGA AGAGCTATTT
    GGACAAGTCA TCAGTGATGA GATCAACTGG CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT
    GCCCAGGATC TGATTACCTT GCTCCTCAGG CAGAATCCCC TGGAGAGACT GGGAACAGGT
    GGTGCATATG AAGTCAAACA GCATCGATTC TTCCGTTGCT TAGACTGGAA CAGTTTGCTG
    AGACAGAAGG CAGAATTCAT TCCCCAACTG GAATCTGAAG ACGACACAAG TTATTTTGAT
    ACTCGGTCAG AGAAGTACCA TCATATGGAG ACTGAGGAAG AAGATGACAC GAACGATGAA
    GACTTTAATG TGGAAATAAG GCAGTTTTCC TCATGTTCAC ATAGGTTTTC TAAGGTCTTC
    AGCAGTATGG ATCGAGGTAC TCAGAATTCA GGAGAAGAGA AGGAAGACCC TGGGGACAAA
    ACTAAAAGCA CAGCTTTGCC GTCCACGGAA ACACTAAGCT GGAGTTCAGA GTATTCTGAA
    ATGCAACAGT TATCCACATC CAACTCTTCA GACACTGAAA GCAACAGACA TAGACGCGGT
    GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA GCAGAGGCAG AACACGACGG GGCCGCTCCC
    CGCCCCAGAG AGCTGCACGA GGAGCCGGAA AAGCCAGCCC TGCCTGCTGC AGAGAATGCT
    CAGGAGGAGC CCGAGGTCAC TACCCCGGCC AGCACCATCA GCAGCTCCAC TCTGTCAGTT
    GGCAGTTTTT CAGAGCACTT GGATCAGATA AATGGACGGA GCGAGTGTGT GGACAGTACA
    GACAATGCCT CCAAGCCCTC CAATGAGCCC GCCTCTCACA TGGCTCGGCA GCGCTTAGAA
    AGCACAGAGA AACAGAAAGC CTCGGGGAAA GTCACAAAGT CCCTTTCTGC CAGTGCCCTG
    TCCCTCATGA TCCCAGGAGA TATGTTTGCT GTCTCTCCGC TGGGAAGTCC GATGTCTCCC
    CATTCCCTGT CCTCGGACCC TTCTTCCTCA AGGGATTCCT CTCCCAGCCG AGATTCTTCA
    GGAGCTTCTG CCAGTCCACA CCAGCCCATT GTGATCCACA GTTTGGGGAA GAACTATGGT
    TTCACCATTC GAGCCATCCG GGTGTACGTG GGAGACAGCG ACATTTACAC AGTGCATCAT
    ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA GCTTGCCAGG CAGGACTGAA AGCTGGGGAT
    CTGATCACAC ACATCAATGG AGAACCGGTG CACGGACTTG TCCACACAGA AGTTATAGAG
    CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA ATCACGACCA CCCCATTTGA AAACACATCA
    ATCAAAACGG GACCGGCCAG GAGAAACAGC TATAAGAGCC GGATGGTGAG GCGGAGCAAG
    AAATCTAAGA AGAAGGAAAG TCTGGAAAGG AGGAGATCCC TTTTCAAAAA ACTAGCTAAG
    CAGCCTTCTC CTTTACTCCA CACCAGCCGA AGTTTCTCCT GCTTGAACCG ATCCCTGTCG
    TCAGGGGAGA GCCTCCCAGG GTCCCCCACT CACAGCTTGT CTCCCCGATC CCCTACACCC
    AGCTACCGTT CCACCCCCGA CTTCCCATCC GGCACTAACT CCTCCCAGAG CAGCTCCCCA
    AGCTCCAGCG CTCCCAACTC CCCAGCAGGG TCAGGACACG TCCGGCCCAG CACGCTCCAT
    GGTCTGGCGC CCAAACTCAG CGGGCAGCGC TACCGGTCAG GAAGGCGGAA GTCAGCTGGC
    AGCATCCCCC TGTCTCCGCT GGCCCGGACA CCCTCTCCGA CCCCACAGCC CACCTCCCCG
    CAGCGGTCCC CATCCCCTCT GCTGGGGCAC TCACTTGGCA ATTCCAAGAT GGCTCAAGCC
    TTTCCCAGCA AAATGCACTC CCCACCCACC ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT
    GCAGAACCCC CTCGGTCCCC GCTGCTCAAG CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC
    TCCTATGGAG GCGACAAGAA GCACCTGTGC TCCCGCAAGC ACAGCCTTGA GGTCACCCAG
    GAGGAGGTGC CGCGGGAGCA GCAACAGCGG GAGGTGACCT TGCAGAGCCT GGAGGAGAAC
    GTGTGCGACG CGCCTGCCCT CAGCCGGGCC AGGCCCGTGG AGCAGGGCTG CCTGAAACGC
    CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG TCTGTGGACG AGCTGGACCG AGAGAAGCTA
    AAGGCCAAGG TGGTGGTGAA GAAACCTGAC GGGCTCTCGG AAAAACAGGA ATCCCGCCCG
    AAGCCCCACG GACTCGGCAG TGACTTGGAA AACCTCTGTG CTTTTAGGCT CGAAGAGAGA
    GACAAGAAAA TCTATCCAAA AGCTTCAGAG AGGTCAAATC ATTTTGAAAG CAAAGCAGCC
    GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT TTGAAAGATG CTCTCCACAA GCAGGCCAAT
    GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG GGGGCAACGG CAGCTGGGGA CCACGGCCAG
    GGCACCTGTG ACTTCAAACG AGCCTCAACG CACAGCACCC TCCAAGACGG CCTCTGTCAC
    ACCACCCACA GGTCCACCTC CGGGAAGGGG GACACCGCAG AGAAGGCCCC CCAGGCCAAG
    GAGGGTCTGC GGTGTGAGAA GTTAGACGGC AAGTTGGCCA ACATCGATTA CCTCCGAAAG
    AAGATGTCCC TTGAAGACAA AGACGAGAGC CTCTGTTCTG TGCTCAAGCC CAAGGTGACA
    GCTGGTGCCC AGGACTGCCT GCCTGGGAGC CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG
    GCCCCGCCAG CCTCCGAGGC CCGCACACCG TTCATCGGCA GCGCCCACGC GGCTCAGCTC
    AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC CTTTCTGGCC GGGTGGACAG TGGAGCGGAG
    AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT GTCCGGAAAA GCCCCTCCGA GTATAAGCTG
    GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC ATTGAAGGCA CCTTGGACAT CGCTCTCCTG
    TCGGGACCTC AGGCCTCCAA GACAGAGCTG CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC
    CCAGGCAGTG ATGTGGAGTC CCCCATGCCC CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG
    GCTGACACTG CGGGTCAGAG AGAGTCCTCC CCTGCCGGCT TCAAGGTGAA CAAATCCTAC
    CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC CGGGGTCTGC AGAGTTCACC GGTGGTTGCC
    CCGCCTGACC CAGAGCTGAA GCTGGACAAG AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG
    ACCATCATGG AGAGCCATCC CCAGCGGGGA GAGGGCCACC CCAGCCAACA CCAAGACCAC
    AGCCCCGACG TTAAGCTCCT TCCCTTCCCA GGGCAGAACC TCCAAGGTGC CAACACGTCT
    AGATCCCGCA GCCCACTCTT GCCTGAAAGT GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA
    GAGTTGTCCG AAAGGGGCCC TTCCATAGTC GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC
    ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT CCCCCAGAAG CTGCTAAACC CAGCGACAAC
    TCCCAAACCC CCCCTGTGGT GGGGAGGACC CGCCTGGATG TCTCCACCCA GACCCAGGCC
    TTGGAGAAAG CATGCGGGCC TTGCGGGAGA GCAAAGCCCA GAGACGGCCG AGAGGAGGTG
    AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG
    CTGGGCGCAG AGAGAGGCCT GGAATCCAAG ATGGAACCTG CTTCTACCAG GTGGTCTCTG
    GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG
    AAAGACGGTC CCAAGGAACC GGAGAGGAAA GAACAACCTC TGCAAAAGTA CCTCACCAGT
    TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG AAAGAACAGC CCCTGCAAAA GCACCTCCCC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACCC GAGAGGAAAG AACAGCCCCT GCAAAAGCAC
    GTCACCAGTA GTTCTCAGCC GGGTCCCAAG GAACCGGAGA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCC CCAGTAGTTC TCAGCCGGGT CCCAAGGAAC CGGAGAGGAA AGAACAGCCC
    CTGCAAAAGC ACGTCACCAG TAGTTCTCAG CTGGGTCCCA AGGAACCCGA GAGGAAAGAA
    CAGCCTCTGC AAAAGCACCT CCCCAGTAGT TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG
    AAAGAACAGT GTTTGCAGAA GCACCTCACC AGTAGCTCTC AGCTGGGTCC CAAGGAACCA
    GAAATGAAAG AACAGCCCCC ACAAAAGCAC GTCACCAGTA GTTCTCAACT GTGTCCCAAG
    GAACTGGAAA GGAAAGAACA ACCTCCACAA AAGCACCTCA CTATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAGAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCGGA AAGGAAAGAA CAGTGTTTGC AGAAGCACTT CACCAGTAGT
    TCTCAGCTGG GTCCCAAGGA ACCAGAAACG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAACCG GAAAGGAAAG AACAGCTTCT GCAAGAGCAC
    CTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAATGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCAGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACGT CACCAGTAGC TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGCCCCAAG
    GAGCCAGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC TTCTGCAAAA GCACCTCACC
    ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAAGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAGCCGGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTATTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAACGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGTTCTCAG
    GTGGGTCCCA AGGATCCGGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAAG
    TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG AAAGAACAGT GTTTGCAAAA GCACCTCACC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACTG GAAAGGAAAG AACAACCTCC ACTAAAGCAT
    GTCACCAGTA GCTCTCAGCT GGGTCCCAGG GAACCAGAAA GGAAAGAACA GCCCCCACAA
    AAGCACCTCA CCAGTAGCTG TCAGCCCCCA CCCCCTACCA AAGAGCTCCC TGGCCTGGCT
    TGTCAGCACT GCATTCTGCC CAGCCACCCT GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT
    GCTATAGACT CCAGCTTCCA GGATGCTCTC AGGCCCACTG CTGTGCGCAG TGACAGCAGC
    AGCCACAAGC CCAGGTCTGA CCCCGACCTG GGCCCTCCAA AGTCTAAGTT CCCAGACCGG
    CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG
    ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT
    CCAGATCCCC AGAACACAGC CCGTGAAGTG ATTGGCCAGG GAGCCAGTGG GTACTCAGGC
    CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC ACAAAGCTGA GACCCACCAG CGCTCGGTGT
    CCCCCAGAGG CACTGGAGAT GCTTGGACAT CCACCAGGGC GAGGGCTCCT AGGGGCCAGT
    GAGTCAGTGG ACCAGAAACT CCCCACCTTC AGGGAAAGGC AAAACCTGTC TCCAAAGGCC
    CCCACACCAT CCACTGTGAA AGATTGTCCC CTTCCGTGCA AACAGACAGA CAGGAGCCCG
    AGTCCTCTGG CCACCAGCAC CAACGCTGGC AAGTCTGAAG GCAAGAAATG CACTGAAGCA
    CTTTACACCC CTGCGGATGG TGAGAAGCTG GGGGCCGGCT TGTCCTCGGT GCACAGCGAG
    GCTGGGCCTA AGGGCACAGA GAAGCCGGCA GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC
    AAGGGGAAAG GACTCAGTCC CCAGAAGCCA CTGACCGAGG CCAGCAAGCC CAGCGGCATG
    AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC TCTCTCCGCT GTGCCACCCT CCCAGAGAAG
    TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG GCCAGACCAG CAGTTCCAGA GACTTCTACC
    ACCTCCAGCG ACACCTCCTC TGCCAAGGCT GCTGGGCCTG CAGCAGAATC CCCAGCCTCC
    AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT GGTGGGGATG GCAGAACCCA AATGACCAAG
    AGCGACTCCT TGCCATGCTT CCGCAGCTCA GCCATCACTC TGGAGTCCCA GCACCCCAGC
    CCTGGCCTGG TGGTGGTCAC CAGCCACCGG GACAGGGCCC TCTCAGTAAC CGCCACCGTG
    GGAGAAACCA AAGGGAAAGA GCCAGCCCCC ACCCAGCCAG CTCAGACCAG GAACCAGAAC
    GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC GCTCCAAGCA CTGAGCGCCC CATCGTTCTT
    CCTTCTGAGA AGGACACTGG GCTGCGACAG AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC
    CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228118
    Clone ID Related Accession (Same CDS sequence) XM_024981452.1
    Accession Version XM_024981452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9361
    ATGATCCAGT CTGCGGTGAA TCACGGACCA GCCTCCAGTT GCCGAACCAG CAACCGGAAA 
    AGCTTGATAG GCAATGGACC ATCCCCAGCA TTGCCTCGGC CCCACTCACC TCTTTCTGCT
    CATGCAGGAA ATAGCCCTCA AGATAGTCCA AGAAATTTCT CCCCCAGTGC CTCTGCTCAT
    TTTTCATTTG CACGGAGGAC TGATGGACGC CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT
    GGCTATGGGA CAAACACTCC CAGCTCTACG GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG
    TTGCATCAGT TACCGTATCA ACCGACACCA GATGAATTAC ACTTCTTATC AAAACATTTT
    TGTACCACTG AAAGCATCAC CACAGAGAAC AGATGCAGAA ACACACCCAT GCGCCCCCGT
    TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC GCCTGCTGTG ACCATGAAAT AATTATGATG
    AACCATGTCT ACAAAGAAAG GTTCCCAAAG GCTACAGCTC AGATGGAAGA GCGCCTAAAG
    GAGATCATCA CCAGCTACTC TCCAGACCAC GTTCTCCCCC TTGCAGATGG AGTGCTCAGC
    TTCACTCACC ATCAGATTAT CGAACTGGCT CGCGATTGTT TGGATAAATC CCACCAGGGT
    CTCATCACCT CACGATACTT CCTTGAATTA CAGCACAAAT TAGATAAGTT ACTACAGGAG
    GCTCACAATC GTTCAGAAAG TGGAGAGCTG GCATTTATTA AACAACTAGT TCGAAAGATC
    CTGATTGTTA TTGCCCGCCC TGCTCGCTTA TTAGAGTGCC TGGAATTTGA TCCTGAAGAA
    TTTTACTACC TATTGGAAGC AGCAGAAGGC CATGCCAAAG AAGGACAGGG TATTAAAACT
    GACATTCCCA GGTACATCAT TAGCCAGCTT GGCCTCAATA AAGATCCTCT GGAAGAAATG
    GCTCAACTGG GAAACTATGA TAGTGGGACA GCAGAAACAC CAGAAACAGA TGAATCAGTG
    AGTAGCTCTA ATGCTTCCCT GAAACTTCGA AGGAAGCCTC GGGAAAGTGA TTTTGAAACG
    ATTAAATTGA TCAGCAATGG AGCCTACGGG GCAGTCTATT TCGTTCGGCA TAAGGAATCC
    CGGCAGAGGT TTGCCATGAA GAAGATTAAC AAACAGAACC TCATCCTCCG AAACCAGATC
    CAGCAGGCCT TTGTGGAACG GGATATCCTG ACTTTTGCAG AAAACCCTTT TGTCGTCAGC
    ATGTACTGCT CCTTTGAGAC CAGGCGCCAC TTGTGCATGG TCATGGAGTA TGTGGAAGGG
    GGTGACTGTG CCACGTTAAT GAAGAACATG GGTCCTCTCC CCGTAGATAT GGCCAGGATG
    TACTTTGCTG AGACGGTCCT GGCCTTGGAA TATCTACATA ATTATGGAAT TGTACATAGG
    GATTTGAAAC CAGACAATTT GCTGGTCACC TCTATGGGGC ACATAAAGCT GACCGATTTT
    GGATTATCCA AGGTGGGACT CATGAGCATG ACTACCAATC TTTATGAGGG TCATATTGAG
    AAGGATGCCC GAGAGTTCCT GGACAAGCAG GTCTGTGGCA CACCTGAATA CATTGCACCA
    GAAGTGATTC TGAGGCAGGG CTACGGGAAG CCAGTGGACT GGTGGGCCAT GGGGATTATC
    CTCTATGAAT TTCTGGTTGG ATGCGTGCCA TTCTTTGGGG ACACTCCAGA AGAGCTATTT
    GGACAAGTCA TCAGTGATGA GATCAACTGG CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT
    GCCCAGGATC TGATTACCTT GCTCCTCAGG CAGAATCCCC TGGAGAGACT GGGAACAGGT
    GGTGCATATG AAGTCAAACA GCATCGATTC TTCCGTTGCT TAGACTGGAA CAGTTTGCTG
    AGACAGAAGG CAGAATTCAT TCCCCAACTG GAATCTGAAG ACGACACAAG TTATTTTGAT
    ACTCGGTCAG AGAAGTACCA TCATATGGAG ACTGAGGAAG AAGATGACAC GAACGATGAA
    GACTTTAATG TGGAAATAAG GCAGTTTTCC TCATGTTCAC ATAGGTTTTC TAAGGTCTTC
    AGCAGTATGG ATCGAGGTAC TCAGAATTCA GGAGAAGAGA AGGAAGACCC TGGGGACAAA
    ACTAAAAGCA CAGCTTTGCC GTCCACGGAA ACACTAAGCT GGAGTTCAGA GTATTCTGAA
    ATGCAACAGT TATCCACATC CAACTCTTCA GACACTGAAA GCAACAGACA TAGACGCGGT
    GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA GCAGAGGCAG AACACGACGG GGCCGCTCCC
    CGCCCCAGAG AGCTGCACGA GGAGCCGGAA AAGCCAGCCC TGCCTGCTGC AGAGAATGCT
    CAGGAGGAGC CCGAGGTCAC TACCCCGGCC AGCACCATCA GCAGCTCCAC TCTGTCAGTT
    GGCAGTTTTT CAGAGCACTT GGATCAGATA AATGGACGGA GCGAGTGTGT GGACAGTACA
    GACAATGCCT CCAAGCCCTC CAATGAGCCC GCCTCTCACA TGGCTCGGCA GCGCTTAGAA
    AGCACAGAGA AACAGAAAGC CTCGGGGAAA GTCACAAAGT CCCTTTCTGC CAGTGCCCTG
    TCCCTCATGA TCCCAGGAGA TATGTTTGCT GTCTCTCCGC TGGGAAGTCC GATGTCTCCC
    CATTCCCTGT CCTCGGACCC TTCTTCCTCA AGGGATTCCT CTCCCAGCCG AGATTCTTCA
    GGAGCTTCTG CCAGTCCACA CCAGCCCATT GTGATCCACA GTTTGGGGAA GAACTATGGT
    TTCACCATTC GAGCCATCCG GGTGTACGTG GGAGACAGCG ACATTTACAC AGTGCATCAT
    ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA GCTTGCCAGG CAGGACTGAA AGCTGGGGAT
    CTGATCACAC ACATCAATGG AGAACCGGTG CACGGACTTG TCCACACAGA AGTTATAGAG
    CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA ATCACGACCA CCCCATTTGA AAACACATCA
    ATCAAAACGG GACCGGCCAG GAGAAACAGC TATAAGAGCC GGATGGTGAG GCGGAGCAAG
    AAATCTAAGA AGAAGGAAAG TCTGGAAAGG AGGAGATCCC TTTTCAAAAA ACTAGCTAAG
    CAGCCTTCTC CTTTACTCCA CACCAGCCGA AGTTTCTCCT GCTTGAACCG ATCCCTGTCG
    TCAGGGGAGA GCCTCCCAGG GTCCCCCACT CACAGCTTGT CTCCCCGATC CCCTACACCC
    AGCTACCGTT CCACCCCCGA CTTCCCATCC GGCACTAACT CCTCCCAGAG CAGCTCCCCA
    AGCTCCAGCG CTCCCAACTC CCCAGCAGGG TCAGGACACG TCCGGCCCAG CACGCTCCAT
    GGTCTGGCGC CCAAACTCAG CGGGCAGCGC TACCGGTCAG GAAGGCGGAA GTCAGCTGGC
    AGCATCCCCC TGTCTCCGCT GGCCCGGACA CCCTCTCCGA CCCCACAGCC CACCTCCCCG
    CAGCGGTCCC CATCCCCTCT GCTGGGGCAC TCACTTGGCA ATTCCAAGAT GGCTCAAGCC
    TTTCCCAGCA AAATGCACTC CCCACCCACC ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT
    GCAGAACCCC CTCGGTCCCC GCTGCTCAAG CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC
    TCCTATGGAG GCGACAAGAA GCACCTGTGC TCCCGCAAGC ACAGCCTTGA GGTCACCCAG
    GAGGAGGTGC CGCGGGAGCA GCAACAGCGG GAGGTGACCT TGCAGAGCCT GGAGGAGAAC
    GTGTGCGACG CGCCTGCCCT CAGCCGGGCC AGGCCCGTGG AGCAGGGCTG CCTGAAACGC
    CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG TCTGTGGACG AGCTGGACCG AGAGAAGCTA
    AAGGCCAAGG TGGTGGTGAA GAAACCTGAC GGGCTCTCGG AAAAACAGGA ATCCCGCCCG
    AAGCCCCACG GACTCGGCAG TGACTTGGAA AACCTCTGTG CTTTTAGGCT CGAAGAGAGA
    GACAAGAAAA TCTATCCAAA AGCTTCAGAG AGGTCAAATC ATTTTGAAAG CAAAGCAGCC
    GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT TTGAAAGATG CTCTCCACAA GCAGGCCAAT
    GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG GGGGCAACGG CAGCTGGGGA CCACGGCCAG
    GGCACCTGTG ACTTCAAACG AGCCTCAACG CACAGCACCC TCCAAGACGG CCTCTGTCAC
    ACCACCCACA GGTCCACCTC CGGGAAGGGG GACACCGCAG AGAAGGCCCC CCAGGCCAAG
    GAGGGTCTGC GGTGTGAGAA GTTAGACGGC AAGTTGGCCA ACATCGATTA CCTCCGAAAG
    AAGATGTCCC TTGAAGACAA AGACGAGAGC CTCTGTTCTG TGCTCAAGCC CAAGGTGACA
    GCTGGTGCCC AGGACTGCCT GCCTGGGAGC CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG
    GCCCCGCCAG CCTCCGAGGC CCGCACACCG TTCATCGGCA GCGCCCACGC GGCTCAGCTC
    AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC CTTTCTGGCC GGGTGGACAG TGGAGCGGAG
    AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT GTCCGGAAAA GCCCCTCCGA GTATAAGCTG
    GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC ATTGAAGGCA CCTTGGACAT CGCTCTCCTG
    TCGGGACCTC AGGCCTCCAA GACAGAGCTG CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC
    CCAGGCAGTG ATGTGGAGTC CCCCATGCCC CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG
    GCTGACACTG CGGGTCAGAG AGAGTCCTCC CCTGCCGGCT TCAAGGTGAA CAAATCCTAC
    CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC CGGGGTCTGC AGAGTTCACC GGTGGTTGCC
    CCGCCTGACC CAGAGCTGAA GCTGGACAAG AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG
    ACCATCATGG AGAGCCATCC CCAGCGGGGA GAGGGCCACC CCAGCCAACA CCAAGACCAC
    AGCCCCGACG TTAAGCTCCT TCCCTTCCCA GGGCAGAACC TCCAAGGTGC CAACACGTCT
    AGATCCCGCA GCCCACTCTT GCCTGAAAGT GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA
    GAGTTGTCCG AAAGGGGCCC TTCCATAGTC GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC
    ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT CCCCCAGAAG CTGCTAAACC CAGCGACAAC
    TCCCAAACCC CCCCTGTGGT GGGGAGGACC CGCCTGGATG TCTCCACCCA GACCCAGGCC
    TTGGAGAAAG CATGCGGGCC TTGCGGGAGA GCAAAGCCCA GAGACGGCCG AGAGGAGGTG
    AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG
    CTGGGCGCAG AGAGAGGCCT GGAATCCAAG ATGGAACCTG CTTCTACCAG GTGGTCTCTG
    GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG
    AAAGACGGTC CCAAGGAACC GGAGAGGAAA GAACAACCTC TGCAAAAGTA CCTCACCAGT
    TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG AAAGAACAGC CCCTGCAAAA GCACCTCCCC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACCC GAGAGGAAAG AACAGCCCCT GCAAAAGCAC
    GTCACCAGTA GTTCTCAGCC GGGTCCCAAG GAACCGGAGA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCC CCAGTAGTTC TCAGCCGGGT CCCAAGGAAC CGGAGAGGAA AGAACAGCCC
    CTGCAAAAGC ACGTCACCAG TAGTTCTCAG CTGGGTCCCA AGGAACCCGA GAGGAAAGAA
    CAGCCTCTGC AAAAGCACCT CCCCAGTAGT TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG
    AAAGAACAGT GTTTGCAGAA GCACCTCACC AGTAGCTCTC AGCTGGGTCC CAAGGAACCA
    GAAATGAAAG AACAGCCCCC ACAAAAGCAC GTCACCAGTA GTTCTCAACT GTGTCCCAAG
    GAACTGGAAA GGAAAGAACA ACCTCCACAA AAGCACCTCA CTATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAGAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCGGA AAGGAAAGAA CAGTGTTTGC AGAAGCACTT CACCAGTAGT
    TCTCAGCTGG GTCCCAAGGA ACCAGAAACG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAACCG GAAAGGAAAG AACAGCTTCT GCAAGAGCAC
    CTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAATGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCAGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACGT CACCAGTAGC TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGCCCCAAG
    GAGCCAGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC TTCTGCAAAA GCACCTCACC
    ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAAGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAGCCGGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTATTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAACGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGTTCTCAG
    GTGGGTCCCA AGGATCCGGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAAG
    TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG AAAGAACAGT GTTTGCAAAA GCACCTCACC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACTG GAAAGGAAAG AACAACCTCC ACTAAAGCAT
    GTCACCAGTA GCTCTCAGCT GGGTCCCAGG GAACCAGAAA GGAAAGAACA GCCCCCACAA
    AAGCACCTCA CCAGTAGCTG TCAGCCCCCA CCCCCTACCA AAGAGCTCCC TGGCCTGGCT
    TGTCAGCACT GCATTCTGCC CAGCCACCCT GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT
    GCTATAGACT CCAGCTTCCA GGATGCTCTC AGGCCCACTG CTGTGCGCAG TGACAGCAGC
    AGCCACAAGC CCAGGTCTGA CCCCGACCTG GGCCCTCCAA AGTCTAAGTT CCCAGACCGG
    CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG
    ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT
    CCAGATCCCC AGAACACAGC CCGTGAAGTG ATTGGCCAGG GAGCCAGTGG GTACTCAGGC
    CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC ACAAAGCTGA GACCCACCAG CGCTCGGTGT
    CCCCCAGAGG CACTGGAGAT GCTTGGACAT CCACCAGGGC GAGGGCTCCT AGGGGCCAGT
    GAGTCAGTGG ACCAGAAACT CCCCACCTTC AGGGAAAGGC AAAACCTGTC TCCAAAGGCC
    CCCACACCAT CCACTGTGAA AGATTGTCCC CTTCCGTGCA AACAGACAGA CAGGAGCCCG
    AGTCCTCTGG CCACCAGCAC CAACGCTGGC AAGTCTGAAG GCAAGAAATG CACTGAAGCA
    CTTTACACCC CTGCGGATGG TGAGAAGCTG GGGGCCGGCT TGTCCTCGGT GCACAGCGAG
    GCTGGGCCTA AGGGCACAGA GAAGCCGGCA GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC
    AAGGGGAAAG GACTCAGTCC CCAGAAGCCA CTGACCGAGG CCAGCAAGCC CAGCGGCATG
    AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC TCTCTCCGCT GTGCCACCCT CCCAGAGAAG
    TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG GCCAGACCAG CAGTTCCAGA GACTTCTACC
    ACCTCCAGCG ACACCTCCTC TGCCAAGGCT GCTGGGCCTG CAGCAGAATC CCCAGCCTCC
    AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT GGTGGGGATG GCAGAACCCA AATGACCAAG
    AGCGACTCCT TGCCATGCTT CCGCAGCTCA GCCATCACTC TGGAGTCCCA GCACCCCAGC
    CCTGGCCTGG TGGTGGTCAC CAGCCACCGG GACAGGGCCC TCTCAGTAAC CGCCACCGTG
    GGAGAAACCA AAGGGAAAGA GCCAGCCCCC ACCCAGCCAG CTCAGACCAG GAACCAGAAC
    GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC GCTCCAAGCA CTGAGCGCCC CATCGTTCTT
    CCTTCTGAGA AGGACACTGG GCTGCGACAG AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC
    CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837220.1
    CDS17..9397
    Translation

    Target ORF information:

    RefSeq Version XM_024981452.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGATCCAGT CTGCGGTGAA TCACGGACCA GCCTCCAGTT GCCGAACCAG CAACCGGAAA 
    AGCTTGATAG GCAATGGACC ATCCCCAGCA TTGCCTCGGC CCCACTCACC TCTTTCTGCT
    CATGCAGGAA ATAGCCCTCA AGATAGTCCA AGAAATTTCT CCCCCAGTGC CTCTGCTCAT
    TTTTCATTTG CACGGAGGAC TGATGGACGC CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT
    GGCTATGGGA CAAACACTCC CAGCTCTACG GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG
    TTGCATCAGT TACCGTATCA ACCGACACCA GATGAATTAC ACTTCTTATC AAAACATTTT
    TGTACCACTG AAAGCATCAC CACAGAGAAC AGATGCAGAA ACACACCCAT GCGCCCCCGT
    TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC GCCTGCTGTG ACCATGAAAT AATTATGATG
    AACCATGTCT ACAAAGAAAG GTTCCCAAAG GCTACAGCTC AGATGGAAGA GCGCCTAAAG
    GAGATCATCA CCAGCTACTC TCCAGACCAC GTTCTCCCCC TTGCAGATGG AGTGCTCAGC
    TTCACTCACC ATCAGATTAT CGAACTGGCT CGCGATTGTT TGGATAAATC CCACCAGGGT
    CTCATCACCT CACGATACTT CCTTGAATTA CAGCACAAAT TAGATAAGTT ACTACAGGAG
    GCTCACAATC GTTCAGAAAG TGGAGAGCTG GCATTTATTA AACAACTAGT TCGAAAGATC
    CTGATTGTTA TTGCCCGCCC TGCTCGCTTA TTAGAGTGCC TGGAATTTGA TCCTGAAGAA
    TTTTACTACC TATTGGAAGC AGCAGAAGGC CATGCCAAAG AAGGACAGGG TATTAAAACT
    GACATTCCCA GGTACATCAT TAGCCAGCTT GGCCTCAATA AAGATCCTCT GGAAGAAATG
    GCTCAACTGG GAAACTATGA TAGTGGGACA GCAGAAACAC CAGAAACAGA TGAATCAGTG
    AGTAGCTCTA ATGCTTCCCT GAAACTTCGA AGGAAGCCTC GGGAAAGTGA TTTTGAAACG
    ATTAAATTGA TCAGCAATGG AGCCTACGGG GCAGTCTATT TCGTTCGGCA TAAGGAATCC
    CGGCAGAGGT TTGCCATGAA GAAGATTAAC AAACAGAACC TCATCCTCCG AAACCAGATC
    CAGCAGGCCT TTGTGGAACG GGATATCCTG ACTTTTGCAG AAAACCCTTT TGTCGTCAGC
    ATGTACTGCT CCTTTGAGAC CAGGCGCCAC TTGTGCATGG TCATGGAGTA TGTGGAAGGG
    GGTGACTGTG CCACGTTAAT GAAGAACATG GGTCCTCTCC CCGTAGATAT GGCCAGGATG
    TACTTTGCTG AGACGGTCCT GGCCTTGGAA TATCTACATA ATTATGGAAT TGTACATAGG
    GATTTGAAAC CAGACAATTT GCTGGTCACC TCTATGGGGC ACATAAAGCT GACCGATTTT
    GGATTATCCA AGGTGGGACT CATGAGCATG ACTACCAATC TTTATGAGGG TCATATTGAG
    AAGGATGCCC GAGAGTTCCT GGACAAGCAG GTCTGTGGCA CACCTGAATA CATTGCACCA
    GAAGTGATTC TGAGGCAGGG CTACGGGAAG CCAGTGGACT GGTGGGCCAT GGGGATTATC
    CTCTATGAAT TTCTGGTTGG ATGCGTGCCA TTCTTTGGGG ACACTCCAGA AGAGCTATTT
    GGACAAGTCA TCAGTGATGA GATCAACTGG CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT
    GCCCAGGATC TGATTACCTT GCTCCTCAGG CAGAATCCCC TGGAGAGACT GGGAACAGGT
    GGTGCATATG AAGTCAAACA GCATCGATTC TTCCGTTGCT TAGACTGGAA CAGTTTGCTG
    AGACAGAAGG CAGAATTCAT TCCCCAACTG GAATCTGAAG ACGACACAAG TTATTTTGAT
    ACTCGGTCAG AGAAGTACCA TCATATGGAG ACTGAGGAAG AAGATGACAC GAACGATGAA
    GACTTTAATG TGGAAATAAG GCAGTTTTCC TCATGTTCAC ATAGGTTTTC TAAGGTCTTC
    AGCAGTATGG ATCGAGGTAC TCAGAATTCA GGAGAAGAGA AGGAAGACCC TGGGGACAAA
    ACTAAAAGCA CAGCTTTGCC GTCCACGGAA ACACTAAGCT GGAGTTCAGA GTATTCTGAA
    ATGCAACAGT TATCCACATC CAACTCTTCA GACACTGAAA GCAACAGACA TAGACGCGGT
    GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA GCAGAGGCAG AACACGACGG GGCCGCTCCC
    CGCCCCAGAG AGCTGCACGA GGAGCCGGAA AAGCCAGCCC TGCCTGCTGC AGAGAATGCT
    CAGGAGGAGC CCGAGGTCAC TACCCCGGCC AGCACCATCA GCAGCTCCAC TCTGTCAGTT
    GGCAGTTTTT CAGAGCACTT GGATCAGATA AATGGACGGA GCGAGTGTGT GGACAGTACA
    GACAATGCCT CCAAGCCCTC CAATGAGCCC GCCTCTCACA TGGCTCGGCA GCGCTTAGAA
    AGCACAGAGA AACAGAAAGC CTCGGGGAAA GTCACAAAGT CCCTTTCTGC CAGTGCCCTG
    TCCCTCATGA TCCCAGGAGA TATGTTTGCT GTCTCTCCGC TGGGAAGTCC GATGTCTCCC
    CATTCCCTGT CCTCGGACCC TTCTTCCTCA AGGGATTCCT CTCCCAGCCG AGATTCTTCA
    GGAGCTTCTG CCAGTCCACA CCAGCCCATT GTGATCCACA GTTTGGGGAA GAACTATGGT
    TTCACCATTC GAGCCATCCG GGTGTACGTG GGAGACAGCG ACATTTACAC AGTGCATCAT
    ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA GCTTGCCAGG CAGGACTGAA AGCTGGGGAT
    CTGATCACAC ACATCAATGG AGAACCGGTG CACGGACTTG TCCACACAGA AGTTATAGAG
    CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA ATCACGACCA CCCCATTTGA AAACACATCA
    ATCAAAACGG GACCGGCCAG GAGAAACAGC TATAAGAGCC GGATGGTGAG GCGGAGCAAG
    AAATCTAAGA AGAAGGAAAG TCTGGAAAGG AGGAGATCCC TTTTCAAAAA ACTAGCTAAG
    CAGCCTTCTC CTTTACTCCA CACCAGCCGA AGTTTCTCCT GCTTGAACCG ATCCCTGTCG
    TCAGGGGAGA GCCTCCCAGG GTCCCCCACT CACAGCTTGT CTCCCCGATC CCCTACACCC
    AGCTACCGTT CCACCCCCGA CTTCCCATCC GGCACTAACT CCTCCCAGAG CAGCTCCCCA
    AGCTCCAGCG CTCCCAACTC CCCAGCAGGG TCAGGACACG TCCGGCCCAG CACGCTCCAT
    GGTCTGGCGC CCAAACTCAG CGGGCAGCGC TACCGGTCAG GAAGGCGGAA GTCAGCTGGC
    AGCATCCCCC TGTCTCCGCT GGCCCGGACA CCCTCTCCGA CCCCACAGCC CACCTCCCCG
    CAGCGGTCCC CATCCCCTCT GCTGGGGCAC TCACTTGGCA ATTCCAAGAT GGCTCAAGCC
    TTTCCCAGCA AAATGCACTC CCCACCCACC ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT
    GCAGAACCCC CTCGGTCCCC GCTGCTCAAG CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC
    TCCTATGGAG GCGACAAGAA GCACCTGTGC TCCCGCAAGC ACAGCCTTGA GGTCACCCAG
    GAGGAGGTGC CGCGGGAGCA GCAACAGCGG GAGGTGACCT TGCAGAGCCT GGAGGAGAAC
    GTGTGCGACG CGCCTGCCCT CAGCCGGGCC AGGCCCGTGG AGCAGGGCTG CCTGAAACGC
    CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG TCTGTGGACG AGCTGGACCG AGAGAAGCTA
    AAGGCCAAGG TGGTGGTGAA GAAACCTGAC GGGCTCTCGG AAAAACAGGA ATCCCGCCCG
    AAGCCCCACG GACTCGGCAG TGACTTGGAA AACCTCTGTG CTTTTAGGCT CGAAGAGAGA
    GACAAGAAAA TCTATCCAAA AGCTTCAGAG AGGTCAAATC ATTTTGAAAG CAAAGCAGCC
    GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT TTGAAAGATG CTCTCCACAA GCAGGCCAAT
    GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG GGGGCAACGG CAGCTGGGGA CCACGGCCAG
    GGCACCTGTG ACTTCAAACG AGCCTCAACG CACAGCACCC TCCAAGACGG CCTCTGTCAC
    ACCACCCACA GGTCCACCTC CGGGAAGGGG GACACCGCAG AGAAGGCCCC CCAGGCCAAG
    GAGGGTCTGC GGTGTGAGAA GTTAGACGGC AAGTTGGCCA ACATCGATTA CCTCCGAAAG
    AAGATGTCCC TTGAAGACAA AGACGAGAGC CTCTGTTCTG TGCTCAAGCC CAAGGTGACA
    GCTGGTGCCC AGGACTGCCT GCCTGGGAGC CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG
    GCCCCGCCAG CCTCCGAGGC CCGCACACCG TTCATCGGCA GCGCCCACGC GGCTCAGCTC
    AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC CTTTCTGGCC GGGTGGACAG TGGAGCGGAG
    AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT GTCCGGAAAA GCCCCTCCGA GTATAAGCTG
    GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC ATTGAAGGCA CCTTGGACAT CGCTCTCCTG
    TCGGGACCTC AGGCCTCCAA GACAGAGCTG CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC
    CCAGGCAGTG ATGTGGAGTC CCCCATGCCC CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG
    GCTGACACTG CGGGTCAGAG AGAGTCCTCC CCTGCCGGCT TCAAGGTGAA CAAATCCTAC
    CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC CGGGGTCTGC AGAGTTCACC GGTGGTTGCC
    CCGCCTGACC CAGAGCTGAA GCTGGACAAG AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG
    ACCATCATGG AGAGCCATCC CCAGCGGGGA GAGGGCCACC CCAGCCAACA CCAAGACCAC
    AGCCCCGACG TTAAGCTCCT TCCCTTCCCA GGGCAGAACC TCCAAGGTGC CAACACGTCT
    AGATCCCGCA GCCCACTCTT GCCTGAAAGT GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA
    GAGTTGTCCG AAAGGGGCCC TTCCATAGTC GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC
    ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT CCCCCAGAAG CTGCTAAACC CAGCGACAAC
    TCCCAAACCC CCCCTGTGGT GGGGAGGACC CGCCTGGATG TCTCCACCCA GACCCAGGCC
    TTGGAGAAAG CATGCGGGCC TTGCGGGAGA GCAAAGCCCA GAGACGGCCG AGAGGAGGTG
    AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG
    CTGGGCGCAG AGAGAGGCCT GGAATCCAAG ATGGAACCTG CTTCTACCAG GTGGTCTCTG
    GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG
    AAAGACGGTC CCAAGGAACC GGAGAGGAAA GAACAACCTC TGCAAAAGTA CCTCACCAGT
    TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG AAAGAACAGC CCCTGCAAAA GCACCTCCCC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACCC GAGAGGAAAG AACAGCCCCT GCAAAAGCAC
    GTCACCAGTA GTTCTCAGCC GGGTCCCAAG GAACCGGAGA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCC CCAGTAGTTC TCAGCCGGGT CCCAAGGAAC CGGAGAGGAA AGAACAGCCC
    CTGCAAAAGC ACGTCACCAG TAGTTCTCAG CTGGGTCCCA AGGAACCCGA GAGGAAAGAA
    CAGCCTCTGC AAAAGCACCT CCCCAGTAGT TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG
    AAAGAACAGT GTTTGCAGAA GCACCTCACC AGTAGCTCTC AGCTGGGTCC CAAGGAACCA
    GAAATGAAAG AACAGCCCCC ACAAAAGCAC GTCACCAGTA GTTCTCAACT GTGTCCCAAG
    GAACTGGAAA GGAAAGAACA ACCTCCACAA AAGCACCTCA CTATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAGAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCGGA AAGGAAAGAA CAGTGTTTGC AGAAGCACTT CACCAGTAGT
    TCTCAGCTGG GTCCCAAGGA ACCAGAAACG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAACCG GAAAGGAAAG AACAGCTTCT GCAAGAGCAC
    CTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTTTGCAG
    AAGCACCTCA CCAGTAGTTC TCAGCTGGGT CCCAAGGAAC CAGAAATGAA AGAACAGCCT
    CTGCAAAAGC ACCTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCAGA AAGGAAAGAA
    CAGCCTCTGC AAAAGCACGT CACCAGTAGC TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGCCCCAAG
    GAGCCAGAAA GGAAAGAACA GCCTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAAC CAGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC TTCTGCAAAA GCACCTCACC
    ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAAGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGTCCCAAG GAGCCGGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC CTCTGCAAAA GCACGTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAGGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTATTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAACGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGTTCTCAG
    GTGGGTCCCA AGGATCCGGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAAG
    TCTCAGCCGG GTCCCAAGGA ACCGGAAAGG AAAGAACAGT GTTTGCAAAA GCACCTCACC
    AGTAGTTCTC AGCCGGGTCC CAAGGAACTG GAAAGGAAAG AACAACCTCC ACTAAAGCAT
    GTCACCAGTA GCTCTCAGCT GGGTCCCAGG GAACCAGAAA GGAAAGAACA GCCCCCACAA
    AAGCACCTCA CCAGTAGCTG TCAGCCCCCA CCCCCTACCA AAGAGCTCCC TGGCCTGGCT
    TGTCAGCACT GCATTCTGCC CAGCCACCCT GCTGCCTCTC TGCTGGGGAG CAAGCCCTGT
    GCTATAGACT CCAGCTTCCA GGATGCTCTC AGGCCCACTG CTGTGCGCAG TGACAGCAGC
    AGCCACAAGC CCAGGTCTGA CCCCGACCTG GGCCCTCCAA AGTCTAAGTT CCCAGACCGG
    CCCCTCTCCT CCCAGAAGCC GAGCGCTGAG GCTGCAGTGA GCAAAGAGCC TGGCGCTCCG
    ACCCTCGGCA GAGCTGGGGT GGGGAGTGGT AAGGGTGTGT CAGAGGAGTT CCCTGTCCTT
    CCAGATCCCC AGAACACAGC CCGTGAAGTG ATTGGCCAGG GAGCCAGTGG GTACTCAGGC
    CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC ACAAAGCTGA GACCCACCAG CGCTCGGTGT
    CCCCCAGAGG CACTGGAGAT GCTTGGACAT CCACCAGGGC GAGGGCTCCT AGGGGCCAGT
    GAGTCAGTGG ACCAGAAACT CCCCACCTTC AGGGAAAGGC AAAACCTGTC TCCAAAGGCC
    CCCACACCAT CCACTGTGAA AGATTGTCCC CTTCCGTGCA AACAGACAGA CAGGAGCCCG
    AGTCCTCTGG CCACCAGCAC CAACGCTGGC AAGTCTGAAG GCAAGAAATG CACTGAAGCA
    CTTTACACCC CTGCGGATGG TGAGAAGCTG GGGGCCGGCT TGTCCTCGGT GCACAGCGAG
    GCTGGGCCTA AGGGCACAGA GAAGCCGGCA GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC
    AAGGGGAAAG GACTCAGTCC CCAGAAGCCA CTGACCGAGG CCAGCAAGCC CAGCGGCATG
    AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC TCTCTCCGCT GTGCCACCCT CCCAGAGAAG
    TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG GCCAGACCAG CAGTTCCAGA GACTTCTACC
    ACCTCCAGCG ACACCTCCTC TGCCAAGGCT GCTGGGCCTG CAGCAGAATC CCCAGCCTCC
    AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT GGTGGGGATG GCAGAACCCA AATGACCAAG
    AGCGACTCCT TGCCATGCTT CCGCAGCTCA GCCATCACTC TGGAGTCCCA GCACCCCAGC
    CCTGGCCTGG TGGTGGTCAC CAGCCACCGG GACAGGGCCC TCTCAGTAAC CGCCACCGTG
    GGAGAAACCA AAGGGAAAGA GCCAGCCCCC ACCCAGCCAG CTCAGACCAG GAACCAGAAC
    GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC GCTCCAAGCA CTGAGCGCCC CATCGTTCTT
    CCTTCTGAGA AGGACACTGG GCTGCGACAG AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC
    CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228119
    Clone ID Related Accession (Same CDS sequence) XM_024981454.1
    Accession Version XM_024981454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
    AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CCAGCAACCG GAAAAGCTTG
    ATAGGCAATG GACCATCCCC AGCATTGCCT CGGCCCCACT CACCTCTTTC TGCTCATGCA
    GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCTGC TCATTTTTCA
    TTTGCACGGA GGACTGATGG ACGCCGCTGG TCCTTGGCTT CTCTCCCCTC CTCTGGCTAT
    GGGACAAACA CTCCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT
    CAGTTACCGT ATCAACCGAC ACCAGATGAA TTACACTTCT TATCAAAACA TTTTTGTACC
    ACTGAAAGCA TCACCACAGA GAACAGATGC AGAAACACAC CCATGCGCCC CCGTTCCCGA
    AGTTTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT
    GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAGCGCCT AAAGGAGATC
    ATCACCAGCT ACTCTCCAGA CCACGTTCTC CCCCTTGCAG ATGGAGTGCT CAGCTTCACT
    CACCATCAGA TTATCGAACT GGCTCGCGAT TGTTTGGATA AATCCCACCA GGGTCTCATC
    ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTACTACA GGAGGCTCAC
    AATCGTTCAG AAAGTGGAGA GCTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTGATT
    GTTATTGCCC GCCCTGCTCG CTTATTAGAG TGCCTGGAAT TTGATCCTGA AGAATTTTAC
    TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACTGACATT
    CCCAGGTACA TCATTAGCCA GCTTGGCCTC AATAAAGATC CTCTGGAAGA AATGGCTCAA
    CTGGGAAACT ATGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC
    TCTAATGCTT CCCTGAAACT TCGAAGGAAG CCTCGGGAAA GTGATTTTGA AACGATTAAA
    TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TATTTCGTTC GGCATAAGGA ATCCCGGCAG
    AGGTTTGCCA TGAAGAAGAT TAACAAACAG AACCTCATCC TCCGAAACCA GATCCAGCAG
    GCCTTTGTGG AACGGGATAT CCTGACTTTT GCAGAAAACC CTTTTGTCGT CAGCATGTAC
    TGCTCCTTTG AGACCAGGCG CCACTTGTGC ATGGTCATGG AGTATGTGGA AGGGGGTGAC
    TGTGCCACGT TAATGAAGAA CATGGGTCCT CTCCCCGTAG ATATGGCCAG GATGTACTTT
    GCTGAGACGG TCCTGGCCTT GGAATATCTA CATAATTATG GAATTGTACA TAGGGATTTG
    AAACCAGACA ATTTGCTGGT CACCTCTATG GGGCACATAA AGCTGACCGA TTTTGGATTA
    TCCAAGGTGG GACTCATGAG CATGACTACC AATCTTTATG AGGGTCATAT TGAGAAGGAT
    GCCCGAGAGT TCCTGGACAA GCAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG
    ATTCTGAGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT
    GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA
    GTCATCAGTG ATGAGATCAA CTGGCCTGAA AAGGATGAGG CTCCACCCCC TGATGCCCAG
    GATCTGATTA CCTTGCTCCT CAGGCAGAAT CCCCTGGAGA GACTGGGAAC AGGTGGTGCA
    TATGAAGTCA AACAGCATCG ATTCTTCCGT TGCTTAGACT GGAACAGTTT GCTGAGACAG
    AAGGCAGAAT TCATTCCCCA ACTGGAATCT GAAGACGACA CAAGTTATTT TGATACTCGG
    TCAGAGAAGT ACCATCATAT GGAGACTGAG GAAGAAGATG ACACGAACGA TGAAGACTTT
    AATGTGGAAA TAAGGCAGTT TTCCTCATGT TCACATAGGT TTTCTAAGGT CTTCAGCAGT
    ATGGATCGAG GTACTCAGAA TTCAGGAGAA GAGAAGGAAG ACCCTGGGGA CAAAACTAAA
    AGCACAGCTT TGCCGTCCAC GGAAACACTA AGCTGGAGTT CAGAGTATTC TGAAATGCAA
    CAGTTATCCA CATCCAACTC TTCAGACACT GAAAGCAACA GACATAGACG CGGTGCTGGG
    CTGCTTCCCA AGCTGGCTGT TTCAGCAGAG GCAGAACACG ACGGGGCCGC TCCCCGCCCC
    AGAGAGCTGC ACGAGGAGCC GGAAAAGCCA GCCCTGCCTG CTGCAGAGAA TGCTCAGGAG
    GAGCCCGAGG TCACTACCCC GGCCAGCACC ATCAGCAGCT CCACTCTGTC AGATATGTTT
    GCTGTCTCTC CGCTGGGAAG TCCGATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCC
    TCAAGGGATT CCTCTCCCAG CCGAGATTCT TCAGGAGCTT CTGCCAGTCC ACACCAGCCC
    ATTGTGATCC ACAGTTTGGG GAAGAACTAT GGTTTCACCA TTCGAGCCAT CCGGGTGTAC
    GTGGGAGACA GCGACATTTA CACAGTGCAT CATATCGTTT GGAATGTAGA AGAAGGAAGT
    CCAGCTTGCC AGGCAGGACT GAAAGCTGGG GATCTGATCA CACACATCAA TGGAGAACCG
    GTGCACGGAC TTGTCCACAC AGAAGTTATA GAGCTCCTGC TAAAGAGTGG AAATAAGGTG
    TCAATCACGA CCACCCCATT TGAAAACACA TCAATCAAAA CGGGACCGGC CAGGAGAAAC
    AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCTA AGAAGAAGGA AAGTCTGGAA
    AGGAGGAGAT CCCTTTTCAA AAAACTAGCT AAGCAGCCTT CTCCTTTACT CCACACCAGC
    CGAAGTTTCT CCTGCTTGAA CCGATCCCTG TCGTCAGGGG AGAGCCTCCC AGGGTCCCCC
    ACTCACAGCT TGTCTCCCCG ATCCCCTACA CCCAGCTACC GTTCCACCCC CGACTTCCCA
    TCCGGCACTA ACTCCTCCCA GAGCAGCTCC CCAAGCTCCA GCGCTCCCAA CTCCCCAGCA
    GGGTCAGGAC ACGTCCGGCC CAGCACGCTC CATGGTCTGG CGCCCAAACT CAGCGGGCAG
    CGCTACCGGT CAGGAAGGCG GAAGTCAGCT GGCAGCATCC CCCTGTCTCC GCTGGCCCGG
    ACACCCTCTC CGACCCCACA GCCCACCTCC CCGCAGCGGT CCCCATCCCC TCTGCTGGGG
    CACTCACTTG GCAATTCCAA GATGGCTCAA GCCTTTCCCA GCAAAATGCA CTCCCCACCC
    ACCATCGTCA GGCACGTCGT GAGGCCCAAG AGTGCAGAAC CCCCTCGGTC CCCGCTGCTC
    AAGCGCGTGC AGTCGGAGGA GAAGCTGTCT CCCTCCTATG GAGGCGACAA GAAGCACCTG
    TGCTCCCGCA AGCACAGCCT TGAGGTCACC CAGGAGGAGG TGCCGCGGGA GCAGCAACAG
    CGGGAGGTGA CCTTGCAGAG CCTGGAGGAG AACGTGTGCG ACGCGCCTGC CCTCAGCCGG
    GCCAGGCCCG TGGAGCAGGG CTGCCTGAAA CGCCCCGTGT CCCGGAAGCT GGGCCGCCAG
    GAGTCTGTGG ACGAGCTGGA CCGAGAGAAG CTAAAGGCCA AGGTGGTGGT GAAGAAACCT
    GACGGGCTCT CGGAAAAACA GGAATCCCGC CCGAAGCCCC ACGGACTCGG CAGTGACTTG
    GAAAACCTCT GTGCTTTTAG GCTCGAAGAG AGAGACAAGA AAATCTATCC AAAAGCTTCA
    GAGAGGTCAA ATCATTTTGA AAGCAAAGCA GCCGTGCAGG AGGCCCAGCC CCTGTGCAGC
    CTTTTGAAAG ATGCTCTCCA CAAGCAGGCC AATGTGCGGG CCAGCGAGGG TGTCGCCTCT
    GAGGGGGCAA CGGCAGCTGG GGACCACGGC CAGGGCACCT GTGACTTCAA ACGAGCCTCA
    ACGCACAGCA CCCTCCAAGA CGGCCTCTGT CACACCACCC ACAGGTCCAC CTCCGGGAAG
    GGGGACACCG CAGAGAAGGC CCCCCAGGCC AAGGAGGGTC TGCGGTGTGA GAAGTTAGAC
    GGCAAGTTGG CCAACATCGA TTACCTCCGA AAGAAGATGT CCCTTGAAGA CAAAGACGAG
    AGCCTCTGTT CTGTGCTCAA GCCCAAGGTG ACAGCTGGTG CCCAGGACTG CCTGCCTGGG
    AGCCTGGGCC GGCCCGTGGG AGGGCAGCAG GAGGCCCCGC CAGCCTCCGA GGCCCGCACA
    CCGTTCATCG GCAGCGCCCA CGCGGCTCAG CTCAGCGCCG TCTCCTTTGT CCCTCTCAAG
    GCCCTTTCTG GCCGGGTGGA CAGTGGAGCG GAGAAGCCGG GCTTGGCTGC CCCAGAGTCG
    CCTGTCCGGA AAAGCCCCTC CGAGTATAAG CTGGAAGGGC GGTCTGTGTC GTGCCTGAAG
    CCCATTGAAG GCACCTTGGA CATCGCTCTC CTGTCGGGAC CTCAGGCCTC CAAGACAGAG
    CTGCCTTCCC CGGAGCCTGC CCAGAGCCCC AGCCCAGGCA GTGATGTGGA GTCCCCCATG
    CCCCTCGCTC TCCCCAGCAG CACAGGGAGG AAGGCTGACA CTGCGGGTCA GAGAGAGTCC
    TCCCCTGCCG GCTTCAAGGT GAACAAATCC TACCTGCTTG AGCCCCGGTT CCCACCGGCC
    AGCCGGGGTC TGCAGAGTTC ACCGGTGGTT GCCCCGCCTG ACCCAGAGCT GAAGCTGGAC
    AAGAAGGTCT CCCAGGCAGC CCGTGCCCCA GCGACCATCA TGGAGAGCCA TCCCCAGCGG
    GGAGAGGGCC ACCCCAGCCA ACACCAAGAC CACAGCCCCG ACGTTAAGCT CCTTCCCTTC
    CCAGGGCAGA ACCTCCAAGG TGCCAACACG TCTAGATCCC GCAGCCCACT CTTGCCTGAA
    AGTGTCCCCC CTAGAGAGAA GCCGAGTGGG AGAGAGTTGT CCGAAAGGGG CCCTTCCATA
    GTCGGGAGCG AGTGGTCAGC TGTGAGGCCC GACATCCGCA GAGACCCCTC CAAGGAGCTG
    TGTCCCCCAG AAGCTGCTAA ACCCAGCGAC AACTCCCAAA CCCCCCCTGT GGTGGGGAGG
    ACCCGCCTGG ATGTCTCCAC CCAGACCCAG GCCTTGGAGA AAGCATGCGG GCCTTGCGGG
    AGAGCAAAGC CCAGAGACGG CCGAGAGGAG GTGAGGCTGC CGGCCAGAGA GGACTCCTCT
    TTGCGCTCTG CGGGGGCTCC TTGTGAGAGA GAGCTGGGCG CAGAGAGAGG CCTGGAATCC
    AAGATGGAAC CTGCTTCTAC CAGGTGGTCT CTGGAGCCGC CCAGGCCAGA GAGTGAGAAG
    AGTGGAAGGC CTTCTGGTTT CCAGTCTGTG CAGAAAGACG GTCCCAAGGA ACCGGAGAGG
    AAAGAACAAC CTCTGCAAAA GTACCTCACC AGTTCTCAGC CGGGTCCCAA GGAACCCGAG
    AGGAAAGAAC AGCCCCTGCA AAAGCACCTC CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CCCGAGAGGA AAGAACAGCC CCTGCAAAAG CACGTCACCA GTAGTTCTCA GCCGGGTCCC
    AAGGAACCGG AGAGGAAAGA ACAGCCTCTG CAAAAGCACC TCCCCAGTAG TTCTCAGCCG
    GGTCCCAAGG AACCGGAGAG GAAAGAACAG CCCCTGCAAA AGCACGTCAC CAGTAGTTCT
    CAGCTGGGTC CCAAGGAACC CGAGAGGAAA GAACAGCCTC TGCAAAAGCA CCTCCCCAGT
    AGTTCTCAGC CGGGTCCCAA GGAACTGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACCTC
    ACCAGTAGCT CTCAGCTGGG TCCCAAGGAA CCAGAAATGA AAGAACAGCC CCCACAAAAG
    CACGTCACCA GTAGTTCTCA ACTGTGTCCC AAGGAACTGG AAAGGAAAGA ACAACCTCCA
    CAAAAGCACC TCACTATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAG AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAACC GGAAAGGAAA
    GAACAGTGTT TGCAGAAGCA CTTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCAGAA
    ACGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA
    CCGGAAAGGA AAGAACAGCT TCTGCAAGAG CACCTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAACCGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACC TCACCAGTAG TTCTCAGCTG
    GGTCCCAAGG AACCAGAAAT GAAAGAACAG CCTCTGCAAA AGCACCTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGCCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCAGTAGCT GTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGCCCC AAGGAGCCAG AAAGGAAAGA ACAGCCTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC AGAAAGGAAA
    GAACAGCTTC TGCAAAAGCA CCTCACCATT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCTTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAAGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACC TCACCATTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA GAACAGCTTC TGCAAAAGCA CCTCACCATT
    AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCGG AAAGGAAAGA ACAGTATTTG
    CAGAAGCACC TCACCAGTAG TTCTCAGCTG GGTCCCAAGG AACCAGAAAC GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CAGTAGTTCT CAGGTGGGTC CCAAGGATCC GGAAAGGAAA
    GAACAGCCCC CACAAAAGCA CCTCACCAGT AAGTCTCAGC CGGGTCCCAA GGAACCGGAA
    AGGAAAGAAC AGTGTTTGCA AAAGCACCTC ACCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CTGGAAAGGA AAGAACAACC TCCACTAAAG CATGTCACCA GTAGCTCTCA GCTGGGTCCC
    AGGGAACCAG AAAGGAAAGA ACAGCCCCCA CAAAAGCACC TCACCAGTAG CTGTCAGCCC
    CCACCCCCTA CCAAAGAGCT CCCTGGCCTG GCTTGTCAGC ACTGCATTCT GCCCAGCCAC
    CCTGCTGCCT CTCTGCTGGG GAGCAAGCCC TGTGCTATAG ACTCCAGCTT CCAGGATGCT
    CTCAGGCCCA CTGCTGTGCG CAGTGACAGC AGCAGCCACA AGCCCAGGTC TGACCCCGAC
    CTGGGCCCTC CAAAGTCTAA GTTCCCAGAC CGGCCCCTCT CCTCCCAGAA GCCGAGCGCT
    GAGGCTGCAG TGAGCAAAGA GCCTGGCGCT CCGACCCTCG GCAGAGCTGG GGTGGGGAGT
    GGTAAGGGTG TGTCAGAGGA GTTCCCTGTC CTTCCAGATC CCCAGAACAC AGCCCGTGAA
    GTGATTGGCC AGGGAGCCAG TGGGTACTCA GGCCCCCTGC CCATGGAAGG GGGTCCTCTA
    GACACAAAGC TGAGACCCAC CAGCGCTCGG TGTCCCCCAG AGGCACTGGA GATGCTTGGA
    CATCCACCAG GGCGAGGGCT CCTAGGGGCC AGTGAGTCAG TGGACCAGAA ACTCCCCACC
    TTCAGGGAAA GGCAAAACCT GTCTCCAAAG GCCCCCACAC CATCCACTGT GAAAGATTGT
    CCCCTTCCGT GCAAACAGAC AGACAGGAGC CCGAGTCCTC TGGCCACCAG CACCAACGCT
    GGCAAGTCTG AAGGCAAGAA ATGCACTGAA GCACTTTACA CCCCTGCGGA TGGTGAGAAG
    CTGGGGGCCG GCTTGTCCTC GGTGCACAGC GAGGCTGGGC CTAAGGGCAC AGAGAAGCCG
    GCAGCAGCCG CTGGGAAGGG CTGGCCAGAG GCCAAGGGGA AAGGACTCAG TCCCCAGAAG
    CCACTGACCG AGGCCAGCAA GCCCAGCGGC ATGAAGCGGT CACCTTCAGC CACGGTGCAG
    AGCTCTCTCC GCTGTGCCAC CCTCCCAGAG AAGTCTCTGA GCTACTCCTG TGGCTTTCCC
    GAGGCCAGAC CAGCAGTTCC AGAGACTTCT ACCACCTCCA GCGACACCTC CTCTGCCAAG
    GCTGCTGGGC CTGCAGCAGA ATCCCCAGCC TCCAGCAGCA GGGACCAGCA GAAACCCCTG
    CCTGGTGGGG ATGGCAGAAC CCAAATGACC AAGAGCGACT CCTTGCCATG CTTCCGCAGC
    TCAGCCATCA CTCTGGAGTC CCAGCACCCC AGCCCTGGCC TGGTGGTGGT CACCAGCCAC
    CGGGACAGGG CCCTCTCAGT AACCGCCACC GTGGGAGAAA CCAAAGGGAA AGAGCCAGCC
    CCCACCCAGC CAGCTCAGAC CAGGAACCAG AACGTGAGCA GAGAGGCGGC CAAGCCGTCC
    CCCGCTCCAA GCACTGAGCG CCCCATCGTT CTTCCTTCTG AGAAGGACAC TGGGCTGCGA
    CAGAGGCGGG GGAAAGAGAG TTTGCGCGGC AGCCCCCACA AAAAGGCCTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837222.1
    CDS343..9576
    Translation

    Target ORF information:

    RefSeq Version XM_024981454.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
    AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CCAGCAACCG GAAAAGCTTG
    ATAGGCAATG GACCATCCCC AGCATTGCCT CGGCCCCACT CACCTCTTTC TGCTCATGCA
    GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCTGC TCATTTTTCA
    TTTGCACGGA GGACTGATGG ACGCCGCTGG TCCTTGGCTT CTCTCCCCTC CTCTGGCTAT
    GGGACAAACA CTCCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT
    CAGTTACCGT ATCAACCGAC ACCAGATGAA TTACACTTCT TATCAAAACA TTTTTGTACC
    ACTGAAAGCA TCACCACAGA GAACAGATGC AGAAACACAC CCATGCGCCC CCGTTCCCGA
    AGTTTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT
    GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAGCGCCT AAAGGAGATC
    ATCACCAGCT ACTCTCCAGA CCACGTTCTC CCCCTTGCAG ATGGAGTGCT CAGCTTCACT
    CACCATCAGA TTATCGAACT GGCTCGCGAT TGTTTGGATA AATCCCACCA GGGTCTCATC
    ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTACTACA GGAGGCTCAC
    AATCGTTCAG AAAGTGGAGA GCTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTGATT
    GTTATTGCCC GCCCTGCTCG CTTATTAGAG TGCCTGGAAT TTGATCCTGA AGAATTTTAC
    TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACTGACATT
    CCCAGGTACA TCATTAGCCA GCTTGGCCTC AATAAAGATC CTCTGGAAGA AATGGCTCAA
    CTGGGAAACT ATGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC
    TCTAATGCTT CCCTGAAACT TCGAAGGAAG CCTCGGGAAA GTGATTTTGA AACGATTAAA
    TTGATCAGCA ATGGAGCCTA CGGGGCAGTC TATTTCGTTC GGCATAAGGA ATCCCGGCAG
    AGGTTTGCCA TGAAGAAGAT TAACAAACAG AACCTCATCC TCCGAAACCA GATCCAGCAG
    GCCTTTGTGG AACGGGATAT CCTGACTTTT GCAGAAAACC CTTTTGTCGT CAGCATGTAC
    TGCTCCTTTG AGACCAGGCG CCACTTGTGC ATGGTCATGG AGTATGTGGA AGGGGGTGAC
    TGTGCCACGT TAATGAAGAA CATGGGTCCT CTCCCCGTAG ATATGGCCAG GATGTACTTT
    GCTGAGACGG TCCTGGCCTT GGAATATCTA CATAATTATG GAATTGTACA TAGGGATTTG
    AAACCAGACA ATTTGCTGGT CACCTCTATG GGGCACATAA AGCTGACCGA TTTTGGATTA
    TCCAAGGTGG GACTCATGAG CATGACTACC AATCTTTATG AGGGTCATAT TGAGAAGGAT
    GCCCGAGAGT TCCTGGACAA GCAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG
    ATTCTGAGGC AGGGCTACGG GAAGCCAGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT
    GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGACACTC CAGAAGAGCT ATTTGGACAA
    GTCATCAGTG ATGAGATCAA CTGGCCTGAA AAGGATGAGG CTCCACCCCC TGATGCCCAG
    GATCTGATTA CCTTGCTCCT CAGGCAGAAT CCCCTGGAGA GACTGGGAAC AGGTGGTGCA
    TATGAAGTCA AACAGCATCG ATTCTTCCGT TGCTTAGACT GGAACAGTTT GCTGAGACAG
    AAGGCAGAAT TCATTCCCCA ACTGGAATCT GAAGACGACA CAAGTTATTT TGATACTCGG
    TCAGAGAAGT ACCATCATAT GGAGACTGAG GAAGAAGATG ACACGAACGA TGAAGACTTT
    AATGTGGAAA TAAGGCAGTT TTCCTCATGT TCACATAGGT TTTCTAAGGT CTTCAGCAGT
    ATGGATCGAG GTACTCAGAA TTCAGGAGAA GAGAAGGAAG ACCCTGGGGA CAAAACTAAA
    AGCACAGCTT TGCCGTCCAC GGAAACACTA AGCTGGAGTT CAGAGTATTC TGAAATGCAA
    CAGTTATCCA CATCCAACTC TTCAGACACT GAAAGCAACA GACATAGACG CGGTGCTGGG
    CTGCTTCCCA AGCTGGCTGT TTCAGCAGAG GCAGAACACG ACGGGGCCGC TCCCCGCCCC
    AGAGAGCTGC ACGAGGAGCC GGAAAAGCCA GCCCTGCCTG CTGCAGAGAA TGCTCAGGAG
    GAGCCCGAGG TCACTACCCC GGCCAGCACC ATCAGCAGCT CCACTCTGTC AGATATGTTT
    GCTGTCTCTC CGCTGGGAAG TCCGATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCC
    TCAAGGGATT CCTCTCCCAG CCGAGATTCT TCAGGAGCTT CTGCCAGTCC ACACCAGCCC
    ATTGTGATCC ACAGTTTGGG GAAGAACTAT GGTTTCACCA TTCGAGCCAT CCGGGTGTAC
    GTGGGAGACA GCGACATTTA CACAGTGCAT CATATCGTTT GGAATGTAGA AGAAGGAAGT
    CCAGCTTGCC AGGCAGGACT GAAAGCTGGG GATCTGATCA CACACATCAA TGGAGAACCG
    GTGCACGGAC TTGTCCACAC AGAAGTTATA GAGCTCCTGC TAAAGAGTGG AAATAAGGTG
    TCAATCACGA CCACCCCATT TGAAAACACA TCAATCAAAA CGGGACCGGC CAGGAGAAAC
    AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCTA AGAAGAAGGA AAGTCTGGAA
    AGGAGGAGAT CCCTTTTCAA AAAACTAGCT AAGCAGCCTT CTCCTTTACT CCACACCAGC
    CGAAGTTTCT CCTGCTTGAA CCGATCCCTG TCGTCAGGGG AGAGCCTCCC AGGGTCCCCC
    ACTCACAGCT TGTCTCCCCG ATCCCCTACA CCCAGCTACC GTTCCACCCC CGACTTCCCA
    TCCGGCACTA ACTCCTCCCA GAGCAGCTCC CCAAGCTCCA GCGCTCCCAA CTCCCCAGCA
    GGGTCAGGAC ACGTCCGGCC CAGCACGCTC CATGGTCTGG CGCCCAAACT CAGCGGGCAG
    CGCTACCGGT CAGGAAGGCG GAAGTCAGCT GGCAGCATCC CCCTGTCTCC GCTGGCCCGG
    ACACCCTCTC CGACCCCACA GCCCACCTCC CCGCAGCGGT CCCCATCCCC TCTGCTGGGG
    CACTCACTTG GCAATTCCAA GATGGCTCAA GCCTTTCCCA GCAAAATGCA CTCCCCACCC
    ACCATCGTCA GGCACGTCGT GAGGCCCAAG AGTGCAGAAC CCCCTCGGTC CCCGCTGCTC
    AAGCGCGTGC AGTCGGAGGA GAAGCTGTCT CCCTCCTATG GAGGCGACAA GAAGCACCTG
    TGCTCCCGCA AGCACAGCCT TGAGGTCACC CAGGAGGAGG TGCCGCGGGA GCAGCAACAG
    CGGGAGGTGA CCTTGCAGAG CCTGGAGGAG AACGTGTGCG ACGCGCCTGC CCTCAGCCGG
    GCCAGGCCCG TGGAGCAGGG CTGCCTGAAA CGCCCCGTGT CCCGGAAGCT GGGCCGCCAG
    GAGTCTGTGG ACGAGCTGGA CCGAGAGAAG CTAAAGGCCA AGGTGGTGGT GAAGAAACCT
    GACGGGCTCT CGGAAAAACA GGAATCCCGC CCGAAGCCCC ACGGACTCGG CAGTGACTTG
    GAAAACCTCT GTGCTTTTAG GCTCGAAGAG AGAGACAAGA AAATCTATCC AAAAGCTTCA
    GAGAGGTCAA ATCATTTTGA AAGCAAAGCA GCCGTGCAGG AGGCCCAGCC CCTGTGCAGC
    CTTTTGAAAG ATGCTCTCCA CAAGCAGGCC AATGTGCGGG CCAGCGAGGG TGTCGCCTCT
    GAGGGGGCAA CGGCAGCTGG GGACCACGGC CAGGGCACCT GTGACTTCAA ACGAGCCTCA
    ACGCACAGCA CCCTCCAAGA CGGCCTCTGT CACACCACCC ACAGGTCCAC CTCCGGGAAG
    GGGGACACCG CAGAGAAGGC CCCCCAGGCC AAGGAGGGTC TGCGGTGTGA GAAGTTAGAC
    GGCAAGTTGG CCAACATCGA TTACCTCCGA AAGAAGATGT CCCTTGAAGA CAAAGACGAG
    AGCCTCTGTT CTGTGCTCAA GCCCAAGGTG ACAGCTGGTG CCCAGGACTG CCTGCCTGGG
    AGCCTGGGCC GGCCCGTGGG AGGGCAGCAG GAGGCCCCGC CAGCCTCCGA GGCCCGCACA
    CCGTTCATCG GCAGCGCCCA CGCGGCTCAG CTCAGCGCCG TCTCCTTTGT CCCTCTCAAG
    GCCCTTTCTG GCCGGGTGGA CAGTGGAGCG GAGAAGCCGG GCTTGGCTGC CCCAGAGTCG
    CCTGTCCGGA AAAGCCCCTC CGAGTATAAG CTGGAAGGGC GGTCTGTGTC GTGCCTGAAG
    CCCATTGAAG GCACCTTGGA CATCGCTCTC CTGTCGGGAC CTCAGGCCTC CAAGACAGAG
    CTGCCTTCCC CGGAGCCTGC CCAGAGCCCC AGCCCAGGCA GTGATGTGGA GTCCCCCATG
    CCCCTCGCTC TCCCCAGCAG CACAGGGAGG AAGGCTGACA CTGCGGGTCA GAGAGAGTCC
    TCCCCTGCCG GCTTCAAGGT GAACAAATCC TACCTGCTTG AGCCCCGGTT CCCACCGGCC
    AGCCGGGGTC TGCAGAGTTC ACCGGTGGTT GCCCCGCCTG ACCCAGAGCT GAAGCTGGAC
    AAGAAGGTCT CCCAGGCAGC CCGTGCCCCA GCGACCATCA TGGAGAGCCA TCCCCAGCGG
    GGAGAGGGCC ACCCCAGCCA ACACCAAGAC CACAGCCCCG ACGTTAAGCT CCTTCCCTTC
    CCAGGGCAGA ACCTCCAAGG TGCCAACACG TCTAGATCCC GCAGCCCACT CTTGCCTGAA
    AGTGTCCCCC CTAGAGAGAA GCCGAGTGGG AGAGAGTTGT CCGAAAGGGG CCCTTCCATA
    GTCGGGAGCG AGTGGTCAGC TGTGAGGCCC GACATCCGCA GAGACCCCTC CAAGGAGCTG
    TGTCCCCCAG AAGCTGCTAA ACCCAGCGAC AACTCCCAAA CCCCCCCTGT GGTGGGGAGG
    ACCCGCCTGG ATGTCTCCAC CCAGACCCAG GCCTTGGAGA AAGCATGCGG GCCTTGCGGG
    AGAGCAAAGC CCAGAGACGG CCGAGAGGAG GTGAGGCTGC CGGCCAGAGA GGACTCCTCT
    TTGCGCTCTG CGGGGGCTCC TTGTGAGAGA GAGCTGGGCG CAGAGAGAGG CCTGGAATCC
    AAGATGGAAC CTGCTTCTAC CAGGTGGTCT CTGGAGCCGC CCAGGCCAGA GAGTGAGAAG
    AGTGGAAGGC CTTCTGGTTT CCAGTCTGTG CAGAAAGACG GTCCCAAGGA ACCGGAGAGG
    AAAGAACAAC CTCTGCAAAA GTACCTCACC AGTTCTCAGC CGGGTCCCAA GGAACCCGAG
    AGGAAAGAAC AGCCCCTGCA AAAGCACCTC CCCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CCCGAGAGGA AAGAACAGCC CCTGCAAAAG CACGTCACCA GTAGTTCTCA GCCGGGTCCC
    AAGGAACCGG AGAGGAAAGA ACAGCCTCTG CAAAAGCACC TCCCCAGTAG TTCTCAGCCG
    GGTCCCAAGG AACCGGAGAG GAAAGAACAG CCCCTGCAAA AGCACGTCAC CAGTAGTTCT
    CAGCTGGGTC CCAAGGAACC CGAGAGGAAA GAACAGCCTC TGCAAAAGCA CCTCCCCAGT
    AGTTCTCAGC CGGGTCCCAA GGAACTGGAA AGGAAAGAAC AGTGTTTGCA GAAGCACCTC
    ACCAGTAGCT CTCAGCTGGG TCCCAAGGAA CCAGAAATGA AAGAACAGCC CCCACAAAAG
    CACGTCACCA GTAGTTCTCA ACTGTGTCCC AAGGAACTGG AAAGGAAAGA ACAACCTCCA
    CAAAAGCACC TCACTATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAG AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAACC GGAAAGGAAA
    GAACAGTGTT TGCAGAAGCA CTTCACCAGT AGTTCTCAGC TGGGTCCCAA GGAACCAGAA
    ACGAAAGAAC AGCCTCTGCA AAAGCACCTC ACCAGTAGCT CTCAGCTGAG TCCCAAGGAA
    CCGGAAAGGA AAGAACAGCT TCTGCAAGAG CACCTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAACCGG AAAGGAAAGA ACAGTGTTTG CAGAAGCACC TCACCAGTAG TTCTCAGCTG
    GGTCCCAAGG AACCAGAAAT GAAAGAACAG CCTCTGCAAA AGCACCTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAACC AGAAAGGAAA GAACAGCCTC TGCAAAAGCA CGTCACCAGT
    AGCTCTCAGC TGAGCCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACCTC
    ACCAGTAGCT GTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGCCCC AAGGAGCCAG AAAGGAAAGA ACAGCCTCTG
    CAAAAGCACC TCACCATTAG CTCTCAGCTG AGTCCCAAGG AACCAGAAAG GAAAGAACAG
    CCTCTGCAAA AGCACGTCAC CAGTAGCTCT CAGCTGAGTC CCAAGGAGCC AGAAAGGAAA
    GAACAGCTTC TGCAAAAGCA CCTCACCATT AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA
    AGGAAAGAAC AGCTTCTGCA AAAGCACCTC ACCATTAGCT CTCAGCTGAG TCCCAAGGAG
    CCGGAAAAGA AAGAACAGCC TCTGCAAAAG CACGTCACCA GTAGCTCTCA GCTGAGTCCC
    AAGGAGCCGG AAAGGAAAGA ACAGCCTCTG CAAAAGCACC TCACCATTAG CTCTCAGCTG
    AGTCCCAAGG AGCCGGAAAG GAAAGAACAG CCTCTGCAAA AGCACGTCAC CAGTAGCTCT
    CAGCTGAGTC CCAAGGAGCC GGAAAGGAAA GAACAGCTTC TGCAAAAGCA CCTCACCATT
    AGCTCTCAGC TGAGTCCCAA GGAGCCGGAA AGGAAAGAAC AGCCTCTGCA AAAGCACGTC
    ACCAGTAGCT CTCAGCTGAG TCCCAAGGAG CCGGAAAGGA AAGAACAGCC TCTGCAAAAG
    CACGTCACCA GTAGCTCTCA GCTGAGTCCC AAGGAACCGG AAAGGAAAGA ACAGTATTTG
    CAGAAGCACC TCACCAGTAG TTCTCAGCTG GGTCCCAAGG AACCAGAAAC GAAAGAACAG
    CCTCTGCAAA AGCACCTCAC CAGTAGTTCT CAGGTGGGTC CCAAGGATCC GGAAAGGAAA
    GAACAGCCCC CACAAAAGCA CCTCACCAGT AAGTCTCAGC CGGGTCCCAA GGAACCGGAA
    AGGAAAGAAC AGTGTTTGCA AAAGCACCTC ACCAGTAGTT CTCAGCCGGG TCCCAAGGAA
    CTGGAAAGGA AAGAACAACC TCCACTAAAG CATGTCACCA GTAGCTCTCA GCTGGGTCCC
    AGGGAACCAG AAAGGAAAGA ACAGCCCCCA CAAAAGCACC TCACCAGTAG CTGTCAGCCC
    CCACCCCCTA CCAAAGAGCT CCCTGGCCTG GCTTGTCAGC ACTGCATTCT GCCCAGCCAC
    CCTGCTGCCT CTCTGCTGGG GAGCAAGCCC TGTGCTATAG ACTCCAGCTT CCAGGATGCT
    CTCAGGCCCA CTGCTGTGCG CAGTGACAGC AGCAGCCACA AGCCCAGGTC TGACCCCGAC
    CTGGGCCCTC CAAAGTCTAA GTTCCCAGAC CGGCCCCTCT CCTCCCAGAA GCCGAGCGCT
    GAGGCTGCAG TGAGCAAAGA GCCTGGCGCT CCGACCCTCG GCAGAGCTGG GGTGGGGAGT
    GGTAAGGGTG TGTCAGAGGA GTTCCCTGTC CTTCCAGATC CCCAGAACAC AGCCCGTGAA
    GTGATTGGCC AGGGAGCCAG TGGGTACTCA GGCCCCCTGC CCATGGAAGG GGGTCCTCTA
    GACACAAAGC TGAGACCCAC CAGCGCTCGG TGTCCCCCAG AGGCACTGGA GATGCTTGGA
    CATCCACCAG GGCGAGGGCT CCTAGGGGCC AGTGAGTCAG TGGACCAGAA ACTCCCCACC
    TTCAGGGAAA GGCAAAACCT GTCTCCAAAG GCCCCCACAC CATCCACTGT GAAAGATTGT
    CCCCTTCCGT GCAAACAGAC AGACAGGAGC CCGAGTCCTC TGGCCACCAG CACCAACGCT
    GGCAAGTCTG AAGGCAAGAA ATGCACTGAA GCACTTTACA CCCCTGCGGA TGGTGAGAAG
    CTGGGGGCCG GCTTGTCCTC GGTGCACAGC GAGGCTGGGC CTAAGGGCAC AGAGAAGCCG
    GCAGCAGCCG CTGGGAAGGG CTGGCCAGAG GCCAAGGGGA AAGGACTCAG TCCCCAGAAG
    CCACTGACCG AGGCCAGCAA GCCCAGCGGC ATGAAGCGGT CACCTTCAGC CACGGTGCAG
    AGCTCTCTCC GCTGTGCCAC CCTCCCAGAG AAGTCTCTGA GCTACTCCTG TGGCTTTCCC
    GAGGCCAGAC CAGCAGTTCC AGAGACTTCT ACCACCTCCA GCGACACCTC CTCTGCCAAG
    GCTGCTGGGC CTGCAGCAGA ATCCCCAGCC TCCAGCAGCA GGGACCAGCA GAAACCCCTG
    CCTGGTGGGG ATGGCAGAAC CCAAATGACC AAGAGCGACT CCTTGCCATG CTTCCGCAGC
    TCAGCCATCA CTCTGGAGTC CCAGCACCCC AGCCCTGGCC TGGTGGTGGT CACCAGCCAC
    CGGGACAGGG CCCTCTCAGT AACCGCCACC GTGGGAGAAA CCAAAGGGAA AGAGCCAGCC
    CCCACCCAGC CAGCTCAGAC CAGGAACCAG AACGTGAGCA GAGAGGCGGC CAAGCCGTCC
    CCCGCTCCAA GCACTGAGCG CCCCATCGTT CTTCCTTCTG AGAAGGACAC TGGGCTGCGA
    CAGAGGCGGG GGAAAGAGAG TTTGCGCGGC AGCCCCCACA AAAAGGCCTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94100
    Clone ID Related Accession (Same CDS sequence) XM_015459062.1
    Accession Version XM_015459062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104507.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC AGCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GGAAGGGCAG CCTCCGAAGA GTCATTTCAA CTCAGCCCGA
    CATCGCCAGA GACTAGTGGA CCCCGCTGCT TCAAAAAAGG AGCTGAGTCT TCCCAGAAGA
    GGAAGTCTTT GCCGAACCAG CAACCGGAAA AGCTTGATAG GCAATGGACC ATCCCCAGCA
    TTGCCTCGGC CCCACTCACC TCTTTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA
    AGAAATTTCT CCCCCAGTGC CTCTGCTCAT TTTTCATTTG CACGGAGGAC TGATGGACGC
    CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT GGCTATGGGA CAAACACTCC CAGCTCTACG
    GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCGTATCA ACCGACACCA
    GATGAATTAC ACTTCTTATC AAAACATTTT TGTACCACTG AAAGCATCAC CACAGAGAAC
    AGATGCAGAA ACACACCCAT GCGCCCCCGT TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC
    GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG
    GCTACAGCTC AGATGGAAGA GCGCCTAAAG GAGATCATCA CCAGCTACTC TCCAGACCAC
    GTTCTCCCCC TTGCAGATGG AGTGCTCAGC TTCACTCACC ATCAGATTAT TGAACTGGCT
    CGCGATTGTT TGGATAAATC CCACCAGGGT CTCATCACCT CACGATACTT CCTTGAATTA
    CAGCACAAAT TAGATAAGTT ACTACAGGAG GCTCACAATC GTTCAGAAAG TGGAGAGCTG
    GCATTTATTA AACAACTAGT TCGAAAGATC CTGATTGTTA TTGCCCGCCC TGCTCGCTTA
    TTAGAGTGCC TGGAATTTGA TCCTGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC
    CATGCCAAAG AAGGACAGGG TATTAAAACT GACATTCCCA GGTACATCAT TAGCCAGCTT
    GGCCTCAATA AAGATCCTCT GGAAGAAATG GCTCAACTGG GAAACTATGA TAGTGGGACA
    GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCTTCCCT GAAACTTCGA
    AGGAAGCCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TCAGCAATGG AGCCTACGGG
    GCAGTCTATT TCGTTCGGCA TAAGGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAC
    AAACAGAACC TCATCCTCCG AAACCAGATC CAGCAGGCCT TTGTGGAACG GGATATCCTG
    ACTTTTGCAG AAAACCCTTT TGTCGTCAGC ATGTACTGCT CCTTTGAGAC CAGGCGCCAC
    TTGTGCATGG TCATGGAGTA TGTGGAAGGG GGTGACTGTG CCACGTTAAT GAAGAACATG
    GGTCCTCTCC CCGTAGATAT GGCCAGGATG TACTTTGCTG AGACGGTCCT GGCCTTGGAA
    TATCTACATA ATTATGGAAT TGTACATAGG GATTTGAAAC CAGACAATTT GCTGGTCACC
    TCTATGGGGC ACATAAAGCT GACCGATTTT GGATTATCCA AGGTGGGACT CATGAGCATG
    ACTACCAATC TTTATGAGGG TCATATTGAG AAGGATGCCC GAGAGTTCCT GGACAAGCAG
    GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG CTACGGGAAG
    CCAGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA
    TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG
    CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT GCCCAGGATC TGATTACCTT GCTCCTCAGG
    CAGAATCCCC TGGAGAGACT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC
    TTCCGTTGCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTCAT TCCCCAACTG
    GAATCTGAAG ACGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTACCA TCATATGGAG
    ACTGAGGAAG AAGATGACAC GAACGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCC
    TCATGTTCAC ATAGGTTTTC TAAGGTCTTC AGCAGTATGG ATCGAGGTAC TCAGAATTCA
    GGAGAAGAGA AGGAAGACCC TGGGGACAAA ACTAAAAGCA CAGCTTTGCC GTCCACGGAA
    ACACTAAGCT GGAGTTCAGA GTATTCTGAA ATGCAACAGT TATCCACATC CAACTCTTCA
    GACACTGAAA GCAACAGACA TAGACGCGGT GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA
    GCAGAGGCAG AACACGACGG GGCCGCTCCC CGCCCCAGAG AGCTGCACGA GGAGCCGGAA
    AAGCCAGCCC TGCCTGCTGC AGAGAATGCT CAGGAGGAGC CCGAGGTCAC TACCCCGGCC
    AGCACCATCA GCAGCTCCAC TCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA
    AATGGACGGA GCGAGTGTGT GGACAGTACA GACAATGCCT CCAAGCCCTC CAATGAGCCC
    GCCTCTCACA TGGCTCGGCA GCGCTTAGAA AGCACAGAGA AACAGAAAGC CTCGGGGAAA
    GTCACAAAGT CCCTTTCTGC CAGTGCCCTG TCCCTCATGA TCCCAGGAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCAGAAA GGAAAGAACA GCTTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCTTCT GCAAAAGCAC
    CTCACCATTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTCTGCAA
    AAGCACGTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTATTTGC AGAAGCACCT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGTTCTC AGGTGGGTCC CAAGGATCCG
    GAAAGGAAAG AACAGCCCCC ACAAAAGCAC CTCACCAGTA AGTCTCAGCC GGGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAA AAGCACCTCA CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC TGGAAAGGAA AGAACAACCT CCACTAAAGC ATGTCACCAG TAGCTCTCAG
    CTGGGTCCCA GGGAACCAGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAGC
    TGTCAGCCCC CACCCCCTAC CAAAGAGCTC CCTGGCCTGG CTTGTCAGCA CTGCATTCTG
    CCCAGCCACC CTGCTGCCTC TCTGCTGGGG AGCAAGCCCT GTGCTATAGA CTCCAGCTTC
    CAGGATGCTC TCAGGCCCAC TGCTGTGCGC AGTGACAGCA GCAGCCACAA GCCCAGGTCT
    GACCCCGACC TGGGCCCTCC AAAGTCTAAG TTCCCAGACC GGCCCCTCTC CTCCCAGAAG
    CCGAGCGCTG AGGCTGCAGT GAGCAAAGAG CCTGGCGCTC CGACCCTCGG CAGATCAGGC
    CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC ACAAAGCTGA GACCCACCAG CGCTCGGTGT
    CCCCCAGAGG CACTGGAGAT GCTTGGACAT CCACCAGGGC GAGGGCTCCT AGGGGCCAGT
    GAGTCAGTGG ACCAGAAACT CCCCACCTTC AGGGAAAGGC AAAACCTGTC TCCAAAGGCC
    CCCACACCAT CCACTGTGAA AGATTGTCCC CTTCCGTGCA AACAGACAGA CAGGAGCCCG
    AGTCCTCTGG CCACCAGCAC CAACGCTGGC AAGTCTGAAG GCAAGAAATG CACTGAAGCA
    CTTTACACCC CTGCGGATGG TGAGAAGCTG GGGGCCGGCT TGTCCTCGGT GCACAGCGAG
    GCTGGGCCTA AGGGCACAGA GAAGCCGGCA GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC
    AAGGGGAAAG GACTCAGTCC CCAGAAGCCA CTGACCGAGG CCAGCAAGCC CAGCGGCATG
    AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC TCTCTCCGCT GTGCCACCCT CCCAGAGAAG
    TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG GCCAGACCAG CAGTTCCAGA GACTTCTACC
    ACCTCCAGCG ACACCTCCTC TGCCAAGGCT GCTGGGCCTG CAGCAGAATC CCCAGCCTCC
    AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT GGTGGGGATG GCAGAACCCA AATGACCAAG
    AGCGACTCCT TGCCATGCTT CCGCAGCTCA GCCATCACTC TGGAGTCCCA GCACCCCAGC
    CCTGGCCTGG TGGTGGTCAC CAGCCACCGG GACAGGGCCC TCTCAGTAAC CGCCACCGTG
    GGAGAAACCA AAGGGAAAGA GCCAGCCCCC ACCCAGCCAG CTCAGACCAG GAACCAGAAC
    GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC GCTCCAAGCA CTGAGCGCCC CATCGTTCTT
    CCTTCTGAGA AGGACACTGG GCTGCGACAG AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC
    CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314548.1
    CDS274..10374
    Translation

    Target ORF information:

    RefSeq Version XM_015459062.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC AGCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GGAAGGGCAG CCTCCGAAGA GTCATTTCAA CTCAGCCCGA
    CATCGCCAGA GACTAGTGGA CCCCGCTGCT TCAAAAAAGG AGCTGAGTCT TCCCAGAAGA
    GGAAGTCTTT GCCGAACCAG CAACCGGAAA AGCTTGATAG GCAATGGACC ATCCCCAGCA
    TTGCCTCGGC CCCACTCACC TCTTTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA
    AGAAATTTCT CCCCCAGTGC CTCTGCTCAT TTTTCATTTG CACGGAGGAC TGATGGACGC
    CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT GGCTATGGGA CAAACACTCC CAGCTCTACG
    GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCGTATCA ACCGACACCA
    GATGAATTAC ACTTCTTATC AAAACATTTT TGTACCACTG AAAGCATCAC CACAGAGAAC
    AGATGCAGAA ACACACCCAT GCGCCCCCGT TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC
    GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG
    GCTACAGCTC AGATGGAAGA GCGCCTAAAG GAGATCATCA CCAGCTACTC TCCAGACCAC
    GTTCTCCCCC TTGCAGATGG AGTGCTCAGC TTCACTCACC ATCAGATTAT TGAACTGGCT
    CGCGATTGTT TGGATAAATC CCACCAGGGT CTCATCACCT CACGATACTT CCTTGAATTA
    CAGCACAAAT TAGATAAGTT ACTACAGGAG GCTCACAATC GTTCAGAAAG TGGAGAGCTG
    GCATTTATTA AACAACTAGT TCGAAAGATC CTGATTGTTA TTGCCCGCCC TGCTCGCTTA
    TTAGAGTGCC TGGAATTTGA TCCTGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC
    CATGCCAAAG AAGGACAGGG TATTAAAACT GACATTCCCA GGTACATCAT TAGCCAGCTT
    GGCCTCAATA AAGATCCTCT GGAAGAAATG GCTCAACTGG GAAACTATGA TAGTGGGACA
    GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCTTCCCT GAAACTTCGA
    AGGAAGCCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TCAGCAATGG AGCCTACGGG
    GCAGTCTATT TCGTTCGGCA TAAGGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAC
    AAACAGAACC TCATCCTCCG AAACCAGATC CAGCAGGCCT TTGTGGAACG GGATATCCTG
    ACTTTTGCAG AAAACCCTTT TGTCGTCAGC ATGTACTGCT CCTTTGAGAC CAGGCGCCAC
    TTGTGCATGG TCATGGAGTA TGTGGAAGGG GGTGACTGTG CCACGTTAAT GAAGAACATG
    GGTCCTCTCC CCGTAGATAT GGCCAGGATG TACTTTGCTG AGACGGTCCT GGCCTTGGAA
    TATCTACATA ATTATGGAAT TGTACATAGG GATTTGAAAC CAGACAATTT GCTGGTCACC
    TCTATGGGGC ACATAAAGCT GACCGATTTT GGATTATCCA AGGTGGGACT CATGAGCATG
    ACTACCAATC TTTATGAGGG TCATATTGAG AAGGATGCCC GAGAGTTCCT GGACAAGCAG
    GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG CTACGGGAAG
    CCAGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA
    TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG
    CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT GCCCAGGATC TGATTACCTT GCTCCTCAGG
    CAGAATCCCC TGGAGAGACT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC
    TTCCGTTGCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTCAT TCCCCAACTG
    GAATCTGAAG ACGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTACCA TCATATGGAG
    ACTGAGGAAG AAGATGACAC GAACGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCC
    TCATGTTCAC ATAGGTTTTC TAAGGTCTTC AGCAGTATGG ATCGAGGTAC TCAGAATTCA
    GGAGAAGAGA AGGAAGACCC TGGGGACAAA ACTAAAAGCA CAGCTTTGCC GTCCACGGAA
    ACACTAAGCT GGAGTTCAGA GTATTCTGAA ATGCAACAGT TATCCACATC CAACTCTTCA
    GACACTGAAA GCAACAGACA TAGACGCGGT GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA
    GCAGAGGCAG AACACGACGG GGCCGCTCCC CGCCCCAGAG AGCTGCACGA GGAGCCGGAA
    AAGCCAGCCC TGCCTGCTGC AGAGAATGCT CAGGAGGAGC CCGAGGTCAC TACCCCGGCC
    AGCACCATCA GCAGCTCCAC TCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA
    AATGGACGGA GCGAGTGTGT GGACAGTACA GACAATGCCT CCAAGCCCTC CAATGAGCCC
    GCCTCTCACA TGGCTCGGCA GCGCTTAGAA AGCACAGAGA AACAGAAAGC CTCGGGGAAA
    GTCACAAAGT CCCTTTCTGC CAGTGCCCTG TCCCTCATGA TCCCAGGAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCAGAAA GGAAAGAACA GCTTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCTTCT GCAAAAGCAC
    CTCACCATTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTCTGCAA
    AAGCACGTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTATTTGC AGAAGCACCT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGTTCTC AGGTGGGTCC CAAGGATCCG
    GAAAGGAAAG AACAGCCCCC ACAAAAGCAC CTCACCAGTA AGTCTCAGCC GGGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAA AAGCACCTCA CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC TGGAAAGGAA AGAACAACCT CCACTAAAGC ATGTCACCAG TAGCTCTCAG
    CTGGGTCCCA GGGAACCAGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAGC
    TGTCAGCCCC CACCCCCTAC CAAAGAGCTC CCTGGCCTGG CTTGTCAGCA CTGCATTCTG
    CCCAGCCACC CTGCTGCCTC TCTGCTGGGG AGCAAGCCCT GTGCTATAGA CTCCAGCTTC
    CAGGATGCTC TCAGGCCCAC TGCTGTGCGC AGTGACAGCA GCAGCCACAA GCCCAGGTCT
    GACCCCGACC TGGGCCCTCC AAAGTCTAAG TTCCCAGACC GGCCCCTCTC CTCCCAGAAG
    CCGAGCGCTG AGGCTGCAGT GAGCAAAGAG CCTGGCGCTC CGACCCTCGG CAGATCAGGC
    CCCCTGCCCA TGGAAGGGGG TCCTCTAGAC ACAAAGCTGA GACCCACCAG CGCTCGGTGT
    CCCCCAGAGG CACTGGAGAT GCTTGGACAT CCACCAGGGC GAGGGCTCCT AGGGGCCAGT
    GAGTCAGTGG ACCAGAAACT CCCCACCTTC AGGGAAAGGC AAAACCTGTC TCCAAAGGCC
    CCCACACCAT CCACTGTGAA AGATTGTCCC CTTCCGTGCA AACAGACAGA CAGGAGCCCG
    AGTCCTCTGG CCACCAGCAC CAACGCTGGC AAGTCTGAAG GCAAGAAATG CACTGAAGCA
    CTTTACACCC CTGCGGATGG TGAGAAGCTG GGGGCCGGCT TGTCCTCGGT GCACAGCGAG
    GCTGGGCCTA AGGGCACAGA GAAGCCGGCA GCAGCCGCTG GGAAGGGCTG GCCAGAGGCC
    AAGGGGAAAG GACTCAGTCC CCAGAAGCCA CTGACCGAGG CCAGCAAGCC CAGCGGCATG
    AAGCGGTCAC CTTCAGCCAC GGTGCAGAGC TCTCTCCGCT GTGCCACCCT CCCAGAGAAG
    TCTCTGAGCT ACTCCTGTGG CTTTCCCGAG GCCAGACCAG CAGTTCCAGA GACTTCTACC
    ACCTCCAGCG ACACCTCCTC TGCCAAGGCT GCTGGGCCTG CAGCAGAATC CCCAGCCTCC
    AGCAGCAGGG ACCAGCAGAA ACCCCTGCCT GGTGGGGATG GCAGAACCCA AATGACCAAG
    AGCGACTCCT TGCCATGCTT CCGCAGCTCA GCCATCACTC TGGAGTCCCA GCACCCCAGC
    CCTGGCCTGG TGGTGGTCAC CAGCCACCGG GACAGGGCCC TCTCAGTAAC CGCCACCGTG
    GGAGAAACCA AAGGGAAAGA GCCAGCCCCC ACCCAGCCAG CTCAGACCAG GAACCAGAAC
    GTGAGCAGAG AGGCGGCCAA GCCGTCCCCC GCTCCAAGCA CTGAGCGCCC CATCGTTCTT
    CCTTCTGAGA AGGACACTGG GCTGCGACAG AGGCGGGGGA AAGAGAGTTT GCGCGGCAGC
    CCCCACAAAA AGGCCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa95085
    Clone ID Related Accession (Same CDS sequence) XM_015468336.1
    Accession Version XM_015468336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product microtubule-associated serine/threonine-protein kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646483.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC AGCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GGAAGGGCAG CCTCCGAAGA GTCATTTCAA CTCAGCCCGA
    CATCGCCAGA GACTAGTGGA CCCCGCTGCT TCAAAAAAGG AGCTGAGTCT TCCCAGAAGA
    GGAAGTCTTT GCCGAACCAG CAACCGGAAA AGCTTGATAG GCAATGGACC ATCCCCAGCA
    TTGCCTCGGC CCCACTCACC TCTTTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA
    AGAAATTTCT CCCCCAGTGC CTCTGCTCAT TTTTCATTTG CACGGAGGAC TGATGGACGC
    CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT GGCTATGGGA CAAACACTCC CAGCTCTACG
    GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCGTATCA ACCGACACCA
    GATGAATTAC ACTTCTTATC AAAACATTTT TGTACCACTG AAAGCATCAC CACAGAGAAC
    AGATGCAGAA ACACACCCAT GCGCCCCCGT TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC
    GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG
    GCTACAGCTC AGATGGAAGA GCGCCTAAAG GAGATCATCA CCAGCTACTC TCCAGACCAC
    GTTCTCCCCC TTGCAGATGG AGTGCTCAGC TTCACTCACC ATCAGATTAT TGAACTGGCT
    CGCGATTGTT TGGATAAATC CCACCAGGGT CTCATCACCT CACGATACTT CCTTGAATTA
    CAGCACAAAT TAGATAAGTT ACTACAGGAG GCTCACAATC GTTCAGAAAG TGGAGAGCTG
    GCATTTATTA AACAACTAGT TCGAAAGATC CTGATTGTTA TTGCCCGCCC TGCTCGCTTA
    TTAGAGTGCC TGGAATTTGA TCCTGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC
    CATGCCAAAG AAGGACAGGG TATTAAAACT GACATTCCCA GGTACATCAT TAGCCAGCTT
    GGCCTCAATA AAGATCCTCT GGAAGAAATG GCTCAACTGG GAAACTATGA TAGTGGGACA
    GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCTTCCCT GAAACTTCGA
    AGGAAGCCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TCAGCAATGG AGCCTACGGG
    GCAGTCTATT TCGTTCGGCA TAAGGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAC
    AAACAGAACC TCATCCTCCG AAACCAGATC CAGCAGGCCT TTGTGGAACG GGATATCCTG
    ACTTTTGCAG AAAACCCTTT TGTCGTCAGC ATGTACTGCT CCTTTGAGAC CAGGCGCCAC
    TTGTGCATGG TCATGGAGTA TGTGGAAGGG GGTGACTGTG CCACGTTAAT GAAGAACATG
    GGTCCTCTCC CCGTAGATAT GGCCAGGATG TACTTTGCTG AGACGGTCCT GGCCTTGGAA
    TATCTACATA ATTATGGAAT TGTACATAGG GATTTGAAAC CAGACAATTT GCTGGTCACC
    TCTATGGGGC ACATAAAGCT GACCGATTTT GGATTATCCA AGGTGGGACT CATGAGCATG
    ACTACCAATC TTTATGAGGG TCATATTGAG AAGGATGCCC GAGAGTTCCT GGACAAGCAG
    GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG CTACGGGAAG
    CCAGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA
    TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG
    CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT GCCCAGGATC TGATTACCTT GCTCCTCAGG
    CAGAATCCCC TGGAGAGACT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC
    TTCCGTTGCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTCAT TCCCCAACTG
    GAATCTGAAG ACGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTACCA TCATATGGAG
    ACTGAGGAAG AAGATGACAC GAACGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCC
    TCATGTTCAC ATAGGTTTTC TAAGGTCTTC AGCAGTATGG ATCGAGGTAC TCAGAATTCA
    GGAGAAGAGA AGGAAGACCC TGGGGACAAA ACTAAAAGCA CAGCTTTGCC GTCCACGGAA
    ACACTAAGCT GGAGTTCAGA GTATTCTGAA ATGCAACAGT TATCCACATC CAACTCTTCA
    GACACTGAAA GCAACAGACA TAGACGCGGT GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA
    GCAGAGGCAG AACACGACGG GGCCGCTCCC CGCCCCAGAG AGCTGCACGA GGAGCCGGAA
    AAGCCAGCCC TGCCTGCTGC AGAGAATGCT CAGGAGGAGC CCGAGGTCAC TACCCCGGCC
    AGCACCATCA GCAGCTCCAC TCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA
    AATGGACGGA GCGAGTGTGT GGACAGTACA GACAATGCCT CCAAGCCCTC CAATGAGCCC
    GCCTCTCACA TGGCTCGGCA GCGCTTAGAA AGCACAGAGA AACAGAAAGC CTCGGGGAAA
    GTCACAAAGT CCCTTTCTGC CAGTGCCCTG TCCCTCATGA TCCCAGGAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCAGAAA GGAAAGAACA GCTTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCTTCT GCAAAAGCAC
    CTCACCATTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTCTGCAA
    AAGCACGTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTATTTGC AGAAGCACCT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGTTCTC AGGTGGGTCC CAAGGATCCG
    GAAAGGAAAG AACAGCCCCC ACAAAAGCAC CTCACCAGTA AGTCTCAGCC GGGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAA AAGCACCTCA CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC TGGAAAGGAA AGAACAACCT CCACTAAAGC ATGTCACCAG TAGCTCTCAG
    CTGGGTCCCA GGGAACCAGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAGC
    TGTCAGCCCC CACCCCCTAC CAAAGAGCTC CCTGGCCTGG CTTGTCAGCA CTGCATTCTG
    CCCAGCCACC CTGCTGCCTC TCTGCTGGGG AGCAAGCCCT GTGCTATAGA CTCCAGCTTC
    CAGGATGCTC TCAGGCCCAC TGCTGTGCGC AGTGACAGCA GCAGCCACAA GCCCAGGTCT
    GACCCCGACC TGGGCCCTCC AAAGTCTAAG TTCCCAGACC GGCCCCTCTC CTCCCAGAAG
    CCGAGCGCTG AGGCTGCAGT GAGCAAAGAG CCTGGCGCTC CGACCCTCGG CAGAGCTGGG
    GTGGGGAGTG GTAAGGGTGT GTCAGAGGAG TTCCTGTCCT TCCAGATCCC CAGAACACAG
    CCCGTGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323822.1
    CDS274..9405
    Translation

    Target ORF information:

    RefSeq Version XM_015468336.1
    Organism Bos taurus(cattle)
    Definition Bos taurus microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGGGGAGA AAGTTTCGGA GGCGCCTGAG CCAGTGCCCC GCGGCTGCAG CAGTCACGGT 
    GCTCCGACTC CCGCCCCTGC GGTGGCTGCC GAGTCCTCGC CCGGCGCTTC CTCGGCGGAG
    TCCTCCTCGG GCTCGGAAAC TCTGTCGGAG GAAGGGGAGC CTGGCTTCTC CCGCGAGCAG
    CCGCCGCCGC CGCCGCCGGG TGGAGCTCGG CCGGCCGCCG CGTGGGCTCC GGCGCTCTCG
    GTGCCGGAGC GTGGAGTCTC AGCGCCGCCG CCGCGCCCGG GAGGAGCCGC GCCACCCGCG
    CCCCGGGGCA GCAGCGCGTC CCAGGAGGAG CAGGACGAGG AGCTTGATCA CATATTATCC
    CCTCCACCTA TGCCATTTCG GAAATGCAGC AACCCAGATG TGGCTTCTGG CCCTGGAAAG
    TCGCTGAAGT TTAAAAGACA GCTGAGTGAG GATGGAAGAC AGCTGCGACG GGGGAGCCTG
    GGAGGTGCTC TGACTGGCAG GTATCTCCTT CCAAACCCGG CTGCGGGGCA AGCCTGGGCC
    ACCTCTGCGG AGACGTCCAA CCTCGTGCGC ATGCGCAGCC AGGCCCTGGG CCAGTCGGCG
    CCCTCGCTCA CCGCCAGCCT GGAAGGGCAG CCTCCGAAGA GTCATTTCAA CTCAGCCCGA
    CATCGCCAGA GACTAGTGGA CCCCGCTGCT TCAAAAAAGG AGCTGAGTCT TCCCAGAAGA
    GGAAGTCTTT GCCGAACCAG CAACCGGAAA AGCTTGATAG GCAATGGACC ATCCCCAGCA
    TTGCCTCGGC CCCACTCACC TCTTTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA
    AGAAATTTCT CCCCCAGTGC CTCTGCTCAT TTTTCATTTG CACGGAGGAC TGATGGACGC
    CGCTGGTCCT TGGCTTCTCT CCCCTCCTCT GGCTATGGGA CAAACACTCC CAGCTCTACG
    GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCGTATCA ACCGACACCA
    GATGAATTAC ACTTCTTATC AAAACATTTT TGTACCACTG AAAGCATCAC CACAGAGAAC
    AGATGCAGAA ACACACCCAT GCGCCCCCGT TCCCGAAGTT TGAGCCCTGG ACGTTCTCCC
    GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG
    GCTACAGCTC AGATGGAAGA GCGCCTAAAG GAGATCATCA CCAGCTACTC TCCAGACCAC
    GTTCTCCCCC TTGCAGATGG AGTGCTCAGC TTCACTCACC ATCAGATTAT TGAACTGGCT
    CGCGATTGTT TGGATAAATC CCACCAGGGT CTCATCACCT CACGATACTT CCTTGAATTA
    CAGCACAAAT TAGATAAGTT ACTACAGGAG GCTCACAATC GTTCAGAAAG TGGAGAGCTG
    GCATTTATTA AACAACTAGT TCGAAAGATC CTGATTGTTA TTGCCCGCCC TGCTCGCTTA
    TTAGAGTGCC TGGAATTTGA TCCTGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC
    CATGCCAAAG AAGGACAGGG TATTAAAACT GACATTCCCA GGTACATCAT TAGCCAGCTT
    GGCCTCAATA AAGATCCTCT GGAAGAAATG GCTCAACTGG GAAACTATGA TAGTGGGACA
    GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCTTCCCT GAAACTTCGA
    AGGAAGCCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TCAGCAATGG AGCCTACGGG
    GCAGTCTATT TCGTTCGGCA TAAGGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAC
    AAACAGAACC TCATCCTCCG AAACCAGATC CAGCAGGCCT TTGTGGAACG GGATATCCTG
    ACTTTTGCAG AAAACCCTTT TGTCGTCAGC ATGTACTGCT CCTTTGAGAC CAGGCGCCAC
    TTGTGCATGG TCATGGAGTA TGTGGAAGGG GGTGACTGTG CCACGTTAAT GAAGAACATG
    GGTCCTCTCC CCGTAGATAT GGCCAGGATG TACTTTGCTG AGACGGTCCT GGCCTTGGAA
    TATCTACATA ATTATGGAAT TGTACATAGG GATTTGAAAC CAGACAATTT GCTGGTCACC
    TCTATGGGGC ACATAAAGCT GACCGATTTT GGATTATCCA AGGTGGGACT CATGAGCATG
    ACTACCAATC TTTATGAGGG TCATATTGAG AAGGATGCCC GAGAGTTCCT GGACAAGCAG
    GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG CTACGGGAAG
    CCAGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA
    TTCTTTGGGG ACACTCCAGA AGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG
    CCTGAAAAGG ATGAGGCTCC ACCCCCTGAT GCCCAGGATC TGATTACCTT GCTCCTCAGG
    CAGAATCCCC TGGAGAGACT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC
    TTCCGTTGCT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTCAT TCCCCAACTG
    GAATCTGAAG ACGACACAAG TTATTTTGAT ACTCGGTCAG AGAAGTACCA TCATATGGAG
    ACTGAGGAAG AAGATGACAC GAACGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCC
    TCATGTTCAC ATAGGTTTTC TAAGGTCTTC AGCAGTATGG ATCGAGGTAC TCAGAATTCA
    GGAGAAGAGA AGGAAGACCC TGGGGACAAA ACTAAAAGCA CAGCTTTGCC GTCCACGGAA
    ACACTAAGCT GGAGTTCAGA GTATTCTGAA ATGCAACAGT TATCCACATC CAACTCTTCA
    GACACTGAAA GCAACAGACA TAGACGCGGT GCTGGGCTGC TTCCCAAGCT GGCTGTTTCA
    GCAGAGGCAG AACACGACGG GGCCGCTCCC CGCCCCAGAG AGCTGCACGA GGAGCCGGAA
    AAGCCAGCCC TGCCTGCTGC AGAGAATGCT CAGGAGGAGC CCGAGGTCAC TACCCCGGCC
    AGCACCATCA GCAGCTCCAC TCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA
    AATGGACGGA GCGAGTGTGT GGACAGTACA GACAATGCCT CCAAGCCCTC CAATGAGCCC
    GCCTCTCACA TGGCTCGGCA GCGCTTAGAA AGCACAGAGA AACAGAAAGC CTCGGGGAAA
    GTCACAAAGT CCCTTTCTGC CAGTGCCCTG TCCCTCATGA TCCCAGGAGA TATGTTTGCT
    GTCTCTCCGC TGGGAAGTCC GATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCCTCA
    AGGGATTCCT CTCCCAGCCG AGATTCTTCA GGAGCTTCTG CCAGTCCACA CCAGCCCATT
    GTGATCCACA GTTTGGGGAA GAACTATGGT TTCACCATTC GAGCCATCCG GGTGTACGTG
    GGAGACAGCG ACATTTACAC AGTGCATCAT ATCGTTTGGA ATGTAGAAGA AGGAAGTCCA
    GCTTGCCAGG CAGGACTGAA AGCTGGGGAT CTGATCACAC ACATCAATGG AGAACCGGTG
    CACGGACTTG TCCACACAGA AGTTATAGAG CTCCTGCTAA AGAGTGGAAA TAAGGTGTCA
    ATCACGACCA CCCCATTTGA AAACACATCA ATCAAAACGG GACCGGCCAG GAGAAACAGC
    TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCTAAGA AGAAGGAAAG TCTGGAAAGG
    AGGAGATCCC TTTTCAAAAA ACTAGCTAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA
    AGTTTCTCCT GCTTGAACCG ATCCCTGTCG TCAGGGGAGA GCCTCCCAGG GTCCCCCACT
    CACAGCTTGT CTCCCCGATC CCCTACACCC AGCTACCGTT CCACCCCCGA CTTCCCATCC
    GGCACTAACT CCTCCCAGAG CAGCTCCCCA AGCTCCAGCG CTCCCAACTC CCCAGCAGGG
    TCAGGACACG TCCGGCCCAG CACGCTCCAT GGTCTGGCGC CCAAACTCAG CGGGCAGCGC
    TACCGGTCAG GAAGGCGGAA GTCAGCTGGC AGCATCCCCC TGTCTCCGCT GGCCCGGACA
    CCCTCTCCGA CCCCACAGCC CACCTCCCCG CAGCGGTCCC CATCCCCTCT GCTGGGGCAC
    TCACTTGGCA ATTCCAAGAT GGCTCAAGCC TTTCCCAGCA AAATGCACTC CCCACCCACC
    ATCGTCAGGC ACGTCGTGAG GCCCAAGAGT GCAGAACCCC CTCGGTCCCC GCTGCTCAAG
    CGCGTGCAGT CGGAGGAGAA GCTGTCTCCC TCCTATGGAG GCGACAAGAA GCACCTGTGC
    TCCCGCAAGC ACAGCCTTGA GGTCACCCAG GAGGAGGTGC CGCGGGAGCA GCAACAGCGG
    GAGGTGACCT TGCAGAGCCT GGAGGAGAAC GTGTGCGACG CGCCTGCCCT CAGCCGGGCC
    AGGCCCGTGG AGCAGGGCTG CCTGAAACGC CCCGTGTCCC GGAAGCTGGG CCGCCAGGAG
    TCTGTGGACG AGCTGGACCG AGAGAAGCTA AAGGCCAAGG TGGTGGTGAA GAAACCTGAC
    GGGCTCTCGG AAAAACAGGA ATCCCGCCCG AAGCCCCACG GACTCGGCAG TGACTTGGAA
    AACCTCTGTG CTTTTAGGCT CGAAGAGAGA GACAAGAAAA TCTATCCAAA AGCTTCAGAG
    AGGTCAAATC ATTTTGAAAG CAAAGCAGCC GTGCAGGAGG CCCAGCCCCT GTGCAGCCTT
    TTGAAAGATG CTCTCCACAA GCAGGCCAAT GTGCGGGCCA GCGAGGGTGT CGCCTCTGAG
    GGGGCAACGG CAGCTGGGGA CCACGGCCAG GGCACCTGTG ACTTCAAACG AGCCTCAACG
    CACAGCACCC TCCAAGACGG CCTCTGTCAC ACCACCCACA GGTCCACCTC CGGGAAGGGG
    GACACCGCAG AGAAGGCCCC CCAGGCCAAG GAGGGTCTGC GGTGTGAGAA GTTAGACGGC
    AAGTTGGCCA ACATCGATTA CCTCCGAAAG AAGATGTCCC TTGAAGACAA AGACGAGAGC
    CTCTGTTCTG TGCTCAAGCC CAAGGTGACA GCTGGTGCCC AGGACTGCCT GCCTGGGAGC
    CTGGGCCGGC CCGTGGGAGG GCAGCAGGAG GCCCCGCCAG CCTCCGAGGC CCGCACACCG
    TTCATCGGCA GCGCCCACGC GGCTCAGCTC AGCGCCGTCT CCTTTGTCCC TCTCAAGGCC
    CTTTCTGGCC GGGTGGACAG TGGAGCGGAG AAGCCGGGCT TGGCTGCCCC AGAGTCGCCT
    GTCCGGAAAA GCCCCTCCGA GTATAAGCTG GAAGGGCGGT CTGTGTCGTG CCTGAAGCCC
    ATTGAAGGCA CCTTGGACAT CGCTCTCCTG TCGGGACCTC AGGCCTCCAA GACAGAGCTG
    CCTTCCCCGG AGCCTGCCCA GAGCCCCAGC CCAGGCAGTG ATGTGGAGTC CCCCATGCCC
    CTCGCTCTCC CCAGCAGCAC AGGGAGGAAG GCTGACACTG CGGGTCAGAG AGAGTCCTCC
    CCTGCCGGCT TCAAGGTGAA CAAATCCTAC CTGCTTGAGC CCCGGTTCCC ACCGGCCAGC
    CGGGGTCTGC AGAGTTCACC GGTGGTTGCC CCGCCTGACC CAGAGCTGAA GCTGGACAAG
    AAGGTCTCCC AGGCAGCCCG TGCCCCAGCG ACCATCATGG AGAGCCATCC CCAGCGGGGA
    GAGGGCCACC CCAGCCAACA CCAAGACCAC AGCCCCGACG TTAAGCTCCT TCCCTTCCCA
    GGGCAGAACC TCCAAGGTGC CAACACGTCT AGATCCCGCA GCCCACTCTT GCCTGAAAGT
    GTCCCCCCTA GAGAGAAGCC GAGTGGGAGA GAGTTGTCCG AAAGGGGCCC TTCCATAGTC
    GGGAGCGAGT GGTCAGCTGT GAGGCCCGAC ATCCGCAGAG ACCCCTCCAA GGAGCTGTGT
    CCCCCAGAAG CTGCTAAACC CAGCGACAAC TCCCAAACCC CCCCTGTGGT GGGGAGGACC
    CGCCTGGATG TCTCCACCCA GACCCAGGCC TTGGAGAAAG CATGCGGGCC TTGCGGGAGA
    GCAAAGCCCA GAGACGGCCG AGAGGAGGTG AGGCTGCCGG CCAGAGAGGA CTCCTCTTTG
    CGCTCTGCGG GGGCTCCTTG TGAGAGAGAG CTGGGCGCAG AGAGAGGCCT GGAATCCAAG
    ATGGAACCTG CTTCTACCAG GTGGTCTCTG GAGCCGCCCA GGCCAGAGAG TGAGAAGAGT
    GGAAGGCCTT CTGGTTTCCA GTCTGTGCAG AAAGACGGTC CCAAGGAACC GGAGAGGAAA
    GAACAACCTC TGCAAAAGTA CCTCACCAGT TCTCAGCCGG GTCCCAAGGA ACCCGAGAGG
    AAAGAACAGC CCCTGCAAAA GCACCTCCCC AGTAGTTCTC AGCCGGGTCC CAAGGAACCC
    GAGAGGAAAG AACAGCCCCT GCAAAAGCAC GTCACCAGTA GTTCTCAGCC GGGTCCCAAG
    GAACCGGAGA GGAAAGAACA GCCTCTGCAA AAGCACCTCC CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC CGGAGAGGAA AGAACAGCCC CTGCAAAAGC ACGTCACCAG TAGTTCTCAG
    CTGGGTCCCA AGGAACCCGA GAGGAAAGAA CAGCCTCTGC AAAAGCACCT CCCCAGTAGT
    TCTCAGCCGG GTCCCAAGGA ACTGGAAAGG AAAGAACAGT GTTTGCAGAA GCACCTCACC
    AGTAGCTCTC AGCTGGGTCC CAAGGAACCA GAAATGAAAG AACAGCCCCC ACAAAAGCAC
    GTCACCAGTA GTTCTCAACT GTGTCCCAAG GAACTGGAAA GGAAAGAACA ACCTCCACAA
    AAGCACCTCA CTATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAGAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTGTTTGC AGAAGCACTT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGCTCTC AGCTGAGTCC CAAGGAACCG
    GAAAGGAAAG AACAGCTTCT GCAAGAGCAC CTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAG AAGCACCTCA CCAGTAGTTC TCAGCTGGGT
    CCCAAGGAAC CAGAAATGAA AGAACAGCCT CTGCAAAAGC ACCTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAACCAGA AAGGAAAGAA CAGCCTCTGC AAAAGCACGT CACCAGTAGC
    TCTCAGCTGA GCCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACCTCACC
    AGTAGCTGTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCCTCT GCAAAAGCAC
    GTCACCAGTA GCTCTCAGCT GAGCCCCAAG GAGCCAGAAA GGAAAGAACA GCCTCTGCAA
    AAGCACCTCA CCATTAGCTC TCAGCTGAGT CCCAAGGAAC CAGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAGCCAGA AAGGAAAGAA
    CAGCTTCTGC AAAAGCACCT CACCATTAGC TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG
    AAAGAACAGC TTCTGCAAAA GCACCTCACC ATTAGCTCTC AGCTGAGTCC CAAGGAGCCG
    GAAAAGAAAG AACAGCCTCT GCAAAAGCAC GTCACCAGTA GCTCTCAGCT GAGTCCCAAG
    GAGCCAGAAA GGAAAGAACA GCTTCTGCAA AAGCACCTCA CCATTAGCTC TCAGCTGAGT
    CCCAAGGAGC CGGAAAGGAA AGAACAGCCT CTGCAAAAGC ACGTCACCAG TAGCTCTCAG
    CTGAGTCCCA AGGAGCCGGA AAGGAAAGAA CAGCTTCTGC AAAAGCACCT CACCATTAGC
    TCTCAGCTGA GTCCCAAGGA GCCGGAAAGG AAAGAACAGC CTCTGCAAAA GCACGTCACC
    AGTAGCTCTC AGCTGAGTCC CAAGGAGCCG GAAAGGAAAG AACAGCTTCT GCAAAAGCAC
    CTCACCATTA GCTCTCAGCT GAGTCCCAAG GAACCGGAAA GGAAAGAACA GTGTCTGCAA
    AAGCACGTCA CCAGTAGCTC TCAGCTGAGT CCCAAGGAGC CGGAAAGGAA AGAACAGCCT
    CTGCAAAAGC ACGTCACCAG TAGCTCTCAG CTGAGTCCCA AGGAACCGGA AAGGAAAGAA
    CAGTATTTGC AGAAGCACCT CACCAGTAGT TCTCAGCTGG GTCCCAAGGA ACCAGAAACG
    AAAGAACAGC CTCTGCAAAA GCACCTCACC AGTAGTTCTC AGGTGGGTCC CAAGGATCCG
    GAAAGGAAAG AACAGCCCCC ACAAAAGCAC CTCACCAGTA AGTCTCAGCC GGGTCCCAAG
    GAACCGGAAA GGAAAGAACA GTGTTTGCAA AAGCACCTCA CCAGTAGTTC TCAGCCGGGT
    CCCAAGGAAC TGGAAAGGAA AGAACAACCT CCACTAAAGC ATGTCACCAG TAGCTCTCAG
    CTGGGTCCCA GGGAACCAGA AAGGAAAGAA CAGCCCCCAC AAAAGCACCT CACCAGTAGC
    TGTCAGCCCC CACCCCCTAC CAAAGAGCTC CCTGGCCTGG CTTGTCAGCA CTGCATTCTG
    CCCAGCCACC CTGCTGCCTC TCTGCTGGGG AGCAAGCCCT GTGCTATAGA CTCCAGCTTC
    CAGGATGCTC TCAGGCCCAC TGCTGTGCGC AGTGACAGCA GCAGCCACAA GCCCAGGTCT
    GACCCCGACC TGGGCCCTCC AAAGTCTAAG TTCCCAGACC GGCCCCTCTC CTCCCAGAAG
    CCGAGCGCTG AGGCTGCAGT GAGCAAAGAG CCTGGCGCTC CGACCCTCGG CAGAGCTGGG
    GTGGGGAGTG GTAAGGGTGT GTCAGAGGAG TTCCTGTCCT TCCAGATCCC CAGAACACAG
    CCCGTGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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