AFAP1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	AFAP1
Entrez Gene ID	534032
Full Name	actin filament associated protein 1
General protein information	Preferred Names actin filament associated protein 1 Names actin filament-associated protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024993298.1	XP_024849066.1	actin filament-associated protein 1 isoform X1
XM_024993299.1	XP_024849067.1	actin filament-associated protein 1 isoform X2
NM_001206044.1	NP_001192973.1	actin filament-associated protein 1
XM_024993297.1	XP_024849065.1	actin filament-associated protein 1 isoform X1

Pathways from BioSystems

Homologies

	LOC709419	XP_002804046.1
Xenopus tropicalis (tropical clawed frog)	afap1	XP_004911301.1
Mus musculus (house mouse)	Afap1	NP_081649.1
Gallus gallus (chicken)	AFAP1	NP_989536.1
Bos taurus (cattle)	AFAP1	NP_001192973.1
Rattus norvegicus (Norway rat)	Afap1	NP_543176.1
Danio rerio (zebrafish)	afap1	XP_005156284.1
Homo sapiens (human)	AFAP1	NP_001128119.1
Pan troglodytes (chimpanzee)	AFAP1	XP_003310294.1
Canis lupus familiaris (dog)	AFAP1	XP_005618516.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AFAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01983	NM_001206044.1 Latest version!	Bos taurus actin filament associated protein 1 (AFAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01983	XM_024993297.1 Latest version!	Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01983	XM_024993298.1 Latest version!	Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa235376	XM_024993299.1 Latest version!	Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFAP1 ( NM_001206044.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192973.1 Bos taurus actin filament associated protein 1 (AFAP1), mRNA.

Price & Availability↑

CloneID	OBa01983
Clone ID Related Accession (Same CDS sequence)	XM_024993298.1 , NM_001206044.1 , XM_024993297.1
Accession Version	NM_001206044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2012-08-31
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH126934.1, DT855151.1, DT811432.1, DT844180.1, AAFC02071217.1 and AAFC03087398.1. On Apr 21, 2011 this sequence version replaced XM_613646.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript alignments.

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TCGAAGGGTA AGAAGCCCCC CGTGGCCTCC 
AATGGCGTCA TGGGCAAAGG CAAGGCTCTG AGCGGGCAGC AGAAAAAGGC TGACTCGGCG 
GCTGGCGTGA AGCGGACGCC TTCGAATGCC GACCAGTACA AATATGGCAA GAACCGGGTG 
GAAGCAGATG CCAAGCGGCT GCAGGCCAAA GAAGAGGAGC TGCTGAGGAG GCGGGAGGGC 
CTGCGAGACA GGCTGGCCCA GCTGCGCAAG GAGAGGAAGG GGCTGAGGGC GGCCATCGAA 
GTGAACACCG GCAGGAAGAC CCAGGTGGTC CTGGAAGAGA AGCTGAAGAA GCTGGAGGAG 
GAGTGTAAGC AGAAGGAGAC AGAGCGCGTC AACCTGGAGC TGGAACTGAC GGAGGTCAAG 
GAGAGCCTCA AGAAGGCCCT GGCTGGCGGC ATCACCCTGG GGCTGGCCAT CGAGCCCCGC 
TCAGGGACGT CCAGCCCACA GTCTCCGGTT TTCAGGCATC GGACTCTGGA GAACTCGCCC 
ATGTCCAGCT GTGAGACCAG CGACGCAGAG GGCCCGCTGC CCGTGAACAG TGCAGCCGTC 
CTGAAGAAGA GCCGGCCGGC CCCTGGCAAC TCGCCCTGCC GGGGCCACGT GCTGCGGAAG 
GCCAAGGAGT GGGAGCTGAA GAACGGGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192973.1
CDS	221..2413
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVITDILLRIQSSRGFEVKDHATKPETPSSLPAPPQMPLPEIPQPWLPPDNGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVVREIYSGCSGPAELECPPPPSSPVHKVELEKKLSSERPSSDGEGVAENGIAMCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLILHKDRADLKTHIASIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEVSASVIQTAKQTFCFMNRRVLSTNPYVGSTSNGYAHPSGTALHYDDVPCISGSSKGKKPPVASNGVMGKGKALSGQQKKADSAAGVKRTPSNADQYKYGKNRVEADAKRLQAKEEELLRRREGLRDRLAQLRKERKGLRAAIEVNTGRKTQVVLEEKLKKLEEECKQKETERVNLELELTEVKESLKKALAGGITLGLAIEPRSGTSSPQSPVFRHRTLENSPMSSCETSDAEGPLPVNSAAVLKKSRPAPGNSPCRGHVLRKAKEWELKNGT

Target ORF information:

RefSeq Version	NM_001206044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 (AFAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206044.1

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TCGAAGGGTA AGAAGCCCCC CGTGGCCTCC 
AATGGCGTCA TGGGCAAAGG CAAGGCTCTG AGCGGGCAGC AGAAAAAGGC TGACTCGGCG 
GCTGGCGTGA AGCGGACGCC TTCGAATGCC GACCAGTACA AATATGGCAA GAACCGGGTG 
GAAGCAGATG CCAAGCGGCT GCAGGCCAAA GAAGAGGAGC TGCTGAGGAG GCGGGAGGGC 
CTGCGAGACA GGCTGGCCCA GCTGCGCAAG GAGAGGAAGG GGCTGAGGGC GGCCATCGAA 
GTGAACACCG GCAGGAAGAC CCAGGTGGTC CTGGAAGAGA AGCTGAAGAA GCTGGAGGAG 
GAGTGTAAGC AGAAGGAGAC AGAGCGCGTC AACCTGGAGC TGGAACTGAC GGAGGTCAAG 
GAGAGCCTCA AGAAGGCCCT GGCTGGCGGC ATCACCCTGG GGCTGGCCAT CGAGCCCCGC 
TCAGGGACGT CCAGCCCACA GTCTCCGGTT TTCAGGCATC GGACTCTGGA GAACTCGCCC 
ATGTCCAGCT GTGAGACCAG CGACGCAGAG GGCCCGCTGC CCGTGAACAG TGCAGCCGTC 
CTGAAGAAGA GCCGGCCGGC CCCTGGCAAC TCGCCCTGCC GGGGCCACGT GCTGCGGAAG 
GCCAAGGAGT GGGAGCTGAA GAACGGGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1 ( XM_024993298.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849066.1 Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa01983
Clone ID Related Accession (Same CDS sequence)	XM_024993298.1 , NM_001206044.1 , XM_024993297.1
Accession Version	XM_024993298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TCGAAGGGTA AGAAGCCCCC CGTGGCCTCC 
AATGGCGTCA TGGGCAAAGG CAAGGCTCTG AGCGGGCAGC AGAAAAAGGC TGACTCGGCG 
GCTGGCGTGA AGCGGACGCC TTCGAATGCC GACCAGTACA AATATGGCAA GAACCGGGTG 
GAAGCAGATG CCAAGCGGCT GCAGGCCAAA GAAGAGGAGC TGCTGAGGAG GCGGGAGGGC 
CTGCGAGACA GGCTGGCCCA GCTGCGCAAG GAGAGGAAGG GGCTGAGGGC GGCCATCGAA 
GTGAACACCG GCAGGAAGAC CCAGGTGGTC CTGGAAGAGA AGCTGAAGAA GCTGGAGGAG 
GAGTGTAAGC AGAAGGAGAC AGAGCGCGTC AACCTGGAGC TGGAACTGAC GGAGGTCAAG 
GAGAGCCTCA AGAAGGCCCT GGCTGGCGGC ATCACCCTGG GGCTGGCCAT CGAGCCCCGC 
TCAGGGACGT CCAGCCCACA GTCTCCGGTT TTCAGGCATC GGACTCTGGA GAACTCGCCC 
ATGTCCAGCT GTGAGACCAG CGACGCAGAG GGCCCGCTGC CCGTGAACAG TGCAGCCGTC 
CTGAAGAAGA GCCGGCCGGC CCCTGGCAAC TCGCCCTGCC GGGGCCACGT GCTGCGGAAG 
GCCAAGGAGT GGGAGCTGAA GAACGGGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849066.1
CDS	93..2285
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVITDILLRIQSSRGFEVKDHATKPETPSSLPAPPQMPLPEIPQPWLPPDNGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVVREIYSGCSGPAELECPPPPSSPVHKVELEKKLSSERPSSDGEGVAENGIAMCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLILHKDRADLKTHIASIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEVSASVIQTAKQTFCFMNRRVLSTNPYVGSTSNGYAHPSGTALHYDDVPCISGSSKGKKPPVASNGVMGKGKALSGQQKKADSAAGVKRTPSNADQYKYGKNRVEADAKRLQAKEEELLRRREGLRDRLAQLRKERKGLRAAIEVNTGRKTQVVLEEKLKKLEEECKQKETERVNLELELTEVKESLKKALAGGITLGLAIEPRSGTSSPQSPVFRHRTLENSPMSSCETSDAEGPLPVNSAAVLKKSRPAPGNSPCRGHVLRKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_024993298.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993298.1

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TCGAAGGGTA AGAAGCCCCC CGTGGCCTCC 
AATGGCGTCA TGGGCAAAGG CAAGGCTCTG AGCGGGCAGC AGAAAAAGGC TGACTCGGCG 
GCTGGCGTGA AGCGGACGCC TTCGAATGCC GACCAGTACA AATATGGCAA GAACCGGGTG 
GAAGCAGATG CCAAGCGGCT GCAGGCCAAA GAAGAGGAGC TGCTGAGGAG GCGGGAGGGC 
CTGCGAGACA GGCTGGCCCA GCTGCGCAAG GAGAGGAAGG GGCTGAGGGC GGCCATCGAA 
GTGAACACCG GCAGGAAGAC CCAGGTGGTC CTGGAAGAGA AGCTGAAGAA GCTGGAGGAG 
GAGTGTAAGC AGAAGGAGAC AGAGCGCGTC AACCTGGAGC TGGAACTGAC GGAGGTCAAG 
GAGAGCCTCA AGAAGGCCCT GGCTGGCGGC ATCACCCTGG GGCTGGCCAT CGAGCCCCGC 
TCAGGGACGT CCAGCCCACA GTCTCCGGTT TTCAGGCATC GGACTCTGGA GAACTCGCCC 
ATGTCCAGCT GTGAGACCAG CGACGCAGAG GGCCCGCTGC CCGTGAACAG TGCAGCCGTC 
CTGAAGAAGA GCCGGCCGGC CCCTGGCAAC TCGCCCTGCC GGGGCCACGT GCTGCGGAAG 
GCCAAGGAGT GGGAGCTGAA GAACGGGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1 ( XM_024993297.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849065.1 Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa01983
Clone ID Related Accession (Same CDS sequence)	XM_024993298.1 , NM_001206044.1 , XM_024993297.1
Accession Version	XM_024993297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TCGAAGGGTA AGAAGCCCCC CGTGGCCTCC 
AATGGCGTCA TGGGCAAAGG CAAGGCTCTG AGCGGGCAGC AGAAAAAGGC TGACTCGGCG 
GCTGGCGTGA AGCGGACGCC TTCGAATGCC GACCAGTACA AATATGGCAA GAACCGGGTG 
GAAGCAGATG CCAAGCGGCT GCAGGCCAAA GAAGAGGAGC TGCTGAGGAG GCGGGAGGGC 
CTGCGAGACA GGCTGGCCCA GCTGCGCAAG GAGAGGAAGG GGCTGAGGGC GGCCATCGAA 
GTGAACACCG GCAGGAAGAC CCAGGTGGTC CTGGAAGAGA AGCTGAAGAA GCTGGAGGAG 
GAGTGTAAGC AGAAGGAGAC AGAGCGCGTC AACCTGGAGC TGGAACTGAC GGAGGTCAAG 
GAGAGCCTCA AGAAGGCCCT GGCTGGCGGC ATCACCCTGG GGCTGGCCAT CGAGCCCCGC 
TCAGGGACGT CCAGCCCACA GTCTCCGGTT TTCAGGCATC GGACTCTGGA GAACTCGCCC 
ATGTCCAGCT GTGAGACCAG CGACGCAGAG GGCCCGCTGC CCGTGAACAG TGCAGCCGTC 
CTGAAGAAGA GCCGGCCGGC CCCTGGCAAC TCGCCCTGCC GGGGCCACGT GCTGCGGAAG 
GCCAAGGAGT GGGAGCTGAA GAACGGGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849065.1
CDS	230..2422
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVITDILLRIQSSRGFEVKDHATKPETPSSLPAPPQMPLPEIPQPWLPPDNGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVVREIYSGCSGPAELECPPPPSSPVHKVELEKKLSSERPSSDGEGVAENGIAMCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLILHKDRADLKTHIASIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEVSASVIQTAKQTFCFMNRRVLSTNPYVGSTSNGYAHPSGTALHYDDVPCISGSSKGKKPPVASNGVMGKGKALSGQQKKADSAAGVKRTPSNADQYKYGKNRVEADAKRLQAKEEELLRRREGLRDRLAQLRKERKGLRAAIEVNTGRKTQVVLEEKLKKLEEECKQKETERVNLELELTEVKESLKKALAGGITLGLAIEPRSGTSSPQSPVFRHRTLENSPMSSCETSDAEGPLPVNSAAVLKKSRPAPGNSPCRGHVLRKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_024993297.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993297.1

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TCGAAGGGTA AGAAGCCCCC CGTGGCCTCC 
AATGGCGTCA TGGGCAAAGG CAAGGCTCTG AGCGGGCAGC AGAAAAAGGC TGACTCGGCG 
GCTGGCGTGA AGCGGACGCC TTCGAATGCC GACCAGTACA AATATGGCAA GAACCGGGTG 
GAAGCAGATG CCAAGCGGCT GCAGGCCAAA GAAGAGGAGC TGCTGAGGAG GCGGGAGGGC 
CTGCGAGACA GGCTGGCCCA GCTGCGCAAG GAGAGGAAGG GGCTGAGGGC GGCCATCGAA 
GTGAACACCG GCAGGAAGAC CCAGGTGGTC CTGGAAGAGA AGCTGAAGAA GCTGGAGGAG 
GAGTGTAAGC AGAAGGAGAC AGAGCGCGTC AACCTGGAGC TGGAACTGAC GGAGGTCAAG 
GAGAGCCTCA AGAAGGCCCT GGCTGGCGGC ATCACCCTGG GGCTGGCCAT CGAGCCCCGC 
TCAGGGACGT CCAGCCCACA GTCTCCGGTT TTCAGGCATC GGACTCTGGA GAACTCGCCC 
ATGTCCAGCT GTGAGACCAG CGACGCAGAG GGCCCGCTGC CCGTGAACAG TGCAGCCGTC 
CTGAAGAAGA GCCGGCCGGC CCCTGGCAAC TCGCCCTGCC GGGGCCACGT GCTGCGGAAG 
GCCAAGGAGT GGGAGCTGAA GAACGGGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1 ( XM_024993299.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849067.1 Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA.

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CloneID	OBa235376
Clone ID Related Accession (Same CDS sequence)	XM_024993299.1
Accession Version	XM_024993299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	actin filament-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TGGGGCCCCG ACGACGACGT TCCCGCGCCC 
CGCAACCGAG GCCTGGGAGA AGAGGCGCTC TATGATAACG CCGTCCTGTA CGATAACTTG 
CCGTCTCCGA AAATCTTTGC TCGCTACCCT CCCGCTGACA GGAAGGCTGC TAGGCCGTCT 
CCTGACAGGC TGTCCTGTAA CCACTACAAA CACCCCGCCT CCTCCGGCCT GCCCTCCTCC 
CCGTCTGTCA CTAACACCTC TTCCGTGGGG AGGGTGTCTC TCGGGCTCAG CTCACAGTCG 
AAGGGTAAGA AGCCCCCCGT GGCCTCCAAT GGCGTCATGG GCAAAGGCAA GGCTCTGAGC 
GGGCAGCAGA AAAAGGCTGA CTCGGCGGCT GGCGTGAAGC GGACGCCTTC GAATGCCGAC 
CAGTACAAAT ATGGCAAGAA CCGGGTGGAA GCAGATGCCA AGCGGCTGCA GGCCAAAGAA 
GAGGAGCTGC TGAGGAGGCG GGAGGGCCTG CGAGACAGGC TGGCCCAGCT GCGCAAGGAG 
AGGAAGGGGC TGAGGGCGGC CATCGAAGTG AACACCGGCA GGAAGACCCA GGTGGTCCTG 
GAAGAGAAGC TGAAGAAGCT GGAGGAGGAG TGTAAGCAGA AGGAGACAGA GCGCGTCAAC 
CTGGAGCTGG AACTGACGGA GGTCAAGGAG AGCCTCAAGA AGGCCCTGGC TGGCGGCATC 
ACCCTGGGGC TGGCCATCGA GCCCCGCTCA GGGACGTCCA GCCCACAGTC TCCGGTTTTC 
AGGCATCGGA CTCTGGAGAA CTCGCCCATG TCCAGCTGTG AGACCAGCGA CGCAGAGGGC 
CCGCTGCCCG TGAACAGTGC AGCCGTCCTG AAGAAGAGCC GGCCGGCCCC TGGCAACTCG 
CCCTGCCGGG GCCACGTGCT GCGGAAGGCC AAGGAGTGGG AGCTGAAGAA CGGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849067.1
CDS	232..2691
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVITDILLRIQSSRGFEVKDHATKPETPSSLPAPPQMPLPEIPQPWLPPDNGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVVREIYSGCSGPAELECPPPPSSPVHKVELEKKLSSERPSSDGEGVAENGIAMCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLILHKDRADLKTHIASIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEVSASVIQTAKQTFCFMNRRVLSTNPYVGSTSNGYAHPSGTALHYDDVPCISGSWGPDDDVPAPRNRGLGEEALYDNAVLYDNLPSPKIFARYPPADRKAARPSPDRLSCNHYKHPASSGLPSSPSVTNTSSVGRVSLGLSSQSKGKKPPVASNGVMGKGKALSGQQKKADSAAGVKRTPSNADQYKYGKNRVEADAKRLQAKEEELLRRREGLRDRLAQLRKERKGLRAAIEVNTGRKTQVVLEEKLKKLEEECKQKETERVNLELELTEVKESLKKALAGGITLGLAIEPRSGTSSPQSPVFRHRTLENSPMSSCETSDAEGPLPVNSAAVLKKSRPAPGNSPCRGHVLRKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_024993299.1
Organism	Bos taurus(cattle)
Definition	Bos taurus actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993299.1

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA CGAGTATCTC 
ACCTCAACTG TCAGGGAGAA AAAGGCAGTC ATCACAGACA TCCTGCTGAG GATACAGTCG 
TCCAGAGGTT TTGAAGTGAA GGATCATGCC ACGAAGCCAG AGACCCCTAG CAGCCTGCCG 
GCCCCTCCCC AGATGCCTTT GCCCGAGATC CCACAGCCGT GGCTGCCACC CGACAATGGG 
CCGCCACCGC TCCCAACGTC CTCTCTCCCG GAAGGCTACT ACGAGGAGGC CGTGCCCCTG 
AGTCCCGGGA AAGCTCCGGA GTACATCACC TCAAATTATG ACTCGGATGC GATGAGTAGC 
TCTTACGAAT CCTACGATGA GGAGGAGGAG GATGGGAAGG GGAAGAAGAC GCGGCACCAG 
TGGCCTTCGG AGGAAGCCTC CATGGACCTG GTGAAGGACG CCAAGATCTG TGCCTTCCTG 
CTGCGGAAGA AGCGCTTCGG GCAGTGGACC AAGCTACTCT GCGTCATCAA AGACACCAAG 
CTACTGTGCT ACAAAAGCTC GAAGGACCAG CAACCCCAGA TGGAGCTGCC GCTGCAAGGC 
TGCAACATCA CCTACATCCC GAAGGACAGC AAGAAGAAGA AGCACGAGCT GAAAATCACC 
CAGCAGGGCA CAGACCCGCT CGTCCTCGCC GTCCAGAGCA AGGAGCAGGC CGAGCAGTGG 
CTGAAGGTGG TCAGAGAGAT CTACAGCGGC TGCAGCGGGC CCGCCGAGCT GGAGTGCCCT 
CCCCCGCCGA GCTCGCCGGT GCACAAGGTG GAGCTGGAGA AGAAACTGTC TTCAGAGAGA 
CCAAGTTCAG ATGGTGAGGG TGTTGCAGAA AATGGAATTG CTATGTGTAA TGGAAAAGAA 
CAAGTTAAGA GGAAAAAAAG TTCCAAATCA GAGGCCAAGG GAACTGTGTC TAAAGTCACT 
GGGAAAAAAA TCACCAAGAT CATCGGTTTG GGAAAGAAAA AGCCGTCGAC GGACGAGCAG 
ACCTCCTCGG CAGAGGAAGA CGTGCCGACA TGTGGCTACC TGAACGTGCT TTCCAACAGC 
CGCTGGCGGG AGCGCTGGTG CCGAGTCAAG GACAACAAGC TCATCCTGCA CAAGGACAGG 
GCCGACCTGA AGACGCACAT CGCCTCCATC CCACTGCGCG GCTGCGAGGT CATCCCGGGC 
CTGGACTCCA AGCACCCCCT GACCTTCCGG CTGCTTCGCA ACGGCCAGGA GGTGGCCGTG 
TTGGAGGCCT CCTCTTCTGA AGACATGGGC AGATGGATCG GGATTCTGCT GGCTGAGACA 
GGGTCCTCGA CGGACCCCGG GGCCCTGCAC TACGACTACA TAGACGTGGA GGTGTCCGCG 
AGCGTCATTC AGACGGCCAA GCAGACCTTC TGCTTTATGA ACCGGCGTGT TCTGTCCACT 
AACCCCTATG TGGGGAGCAC CTCCAATGGC TACGCGCACC CCAGCGGGAC GGCGCTTCAC 
TATGATGACG TCCCCTGCAT CAGCGGTTCG TGGGGCCCCG ACGACGACGT TCCCGCGCCC 
CGCAACCGAG GCCTGGGAGA AGAGGCGCTC TATGATAACG CCGTCCTGTA CGATAACTTG 
CCGTCTCCGA AAATCTTTGC TCGCTACCCT CCCGCTGACA GGAAGGCTGC TAGGCCGTCT 
CCTGACAGGC TGTCCTGTAA CCACTACAAA CACCCCGCCT CCTCCGGCCT GCCCTCCTCC 
CCGTCTGTCA CTAACACCTC TTCCGTGGGG AGGGTGTCTC TCGGGCTCAG CTCACAGTCG 
AAGGGTAAGA AGCCCCCCGT GGCCTCCAAT GGCGTCATGG GCAAAGGCAA GGCTCTGAGC 
GGGCAGCAGA AAAAGGCTGA CTCGGCGGCT GGCGTGAAGC GGACGCCTTC GAATGCCGAC 
CAGTACAAAT ATGGCAAGAA CCGGGTGGAA GCAGATGCCA AGCGGCTGCA GGCCAAAGAA 
GAGGAGCTGC TGAGGAGGCG GGAGGGCCTG CGAGACAGGC TGGCCCAGCT GCGCAAGGAG 
AGGAAGGGGC TGAGGGCGGC CATCGAAGTG AACACCGGCA GGAAGACCCA GGTGGTCCTG 
GAAGAGAAGC TGAAGAAGCT GGAGGAGGAG TGTAAGCAGA AGGAGACAGA GCGCGTCAAC 
CTGGAGCTGG AACTGACGGA GGTCAAGGAG AGCCTCAAGA AGGCCCTGGC TGGCGGCATC 
ACCCTGGGGC TGGCCATCGA GCCCCGCTCA GGGACGTCCA GCCCACAGTC TCCGGTTTTC 
AGGCATCGGA CTCTGGAGAA CTCGCCCATG TCCAGCTGTG AGACCAGCGA CGCAGAGGGC 
CCGCTGCCCG TGAACAGTGC AGCCGTCCTG AAGAAGAGCC GGCCGGCCCC TGGCAACTCG 
CCCTGCCGGG GCCACGTGCT GCGGAAGGCC AAGGAGTGGG AGCTGAAGAA CGGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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AFAP1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

REQUEST A QUOTE

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