ROBO2 cDNA ORF clone, Bos taurus(cattle)

The following ROBO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219056 XM_024993907.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219057 XM_024993910.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219058 XM_024993915.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219059 XM_024993918.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219060 XM_024993922.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87636 XM_015468542.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa87636 XM_015461935.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa219054 XM_024993901.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa219055 XM_024993905.1
Latest version!
Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219056
    Clone ID Related Accession (Same CDS sequence) XM_024993907.1
    Accession Version XM_024993907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGATCT TCCACCACCA
    CCAGATCCCC CTCCAGGTCA GGGTTTAAGG CAGCAGATAG GCTCGAGCCA GCATGCTGGT
    AACGTGGAAA ATTCAACAGA GAGAAAAGGA AGCTCTCTGG AGAGACAGCA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC TTTGGATTGT CCAGCTAAAA CATCCCTAGA GTGGCAGCGA
    CAAACCCAGG AATGGATAAA CTCCACTGAA CGCCCAGAAG ACATACGGAA AGCCCTCCAC
    AAACAAGGTG TCGGACCAGA GGAGACATTG GTGCCCTACA GCAAGCCCAA TTTCCCATCT
    CCAGGGGGTC ACAGCTCGTC AGGAACGGCT TCTTCTAAGG GATCCACTGG ACCTAGGAAA
    GCCGATGCTT TGAGGGGAAG CCACCAACGC AATGCCAGCG ACCTTCTTGA CATAGGATAT
    ATGGGCTCAA GTAGTCAAGG ACAGTTTACA GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849675.1
    CDS699..5120
    Translation

    Target ORF information:

    RefSeq Version XM_024993907.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGATCT TCCACCACCA
    CCAGATCCCC CTCCAGGTCA GGGTTTAAGG CAGCAGATAG GCTCGAGCCA GCATGCTGGT
    AACGTGGAAA ATTCAACAGA GAGAAAAGGA AGCTCTCTGG AGAGACAGCA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC TTTGGATTGT CCAGCTAAAA CATCCCTAGA GTGGCAGCGA
    CAAACCCAGG AATGGATAAA CTCCACTGAA CGCCCAGAAG ACATACGGAA AGCCCTCCAC
    AAACAAGGTG TCGGACCAGA GGAGACATTG GTGCCCTACA GCAAGCCCAA TTTCCCATCT
    CCAGGGGGTC ACAGCTCGTC AGGAACGGCT TCTTCTAAGG GATCCACTGG ACCTAGGAAA
    GCCGATGCTT TGAGGGGAAG CCACCAACGC AATGCCAGCG ACCTTCTTGA CATAGGATAT
    ATGGGCTCAA GTAGTCAAGG ACAGTTTACA GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219057
    Clone ID Related Accession (Same CDS sequence) XM_024993910.1
    Accession Version XM_024993910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAAGT TTTCTAGTCA TAGATCTAGC
    GTAGGTAGCT CCTCAGATGG TTCCATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGTAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGCGGGCGA
    GTGGACCCGC AACCAGCATT GCCTCACCGA AGGGAAGGAA TGACAGATGA GGAGACATTG
    GTGCCCTACA GCAAGCCCAA TTTCCCATCT CCAGGGGGTC ACAGCTCGTC AGGAACGGCT
    TCTTCTAAGG GATCCACTGG ACCTAGGAAA GCCGATGCTT TGAGGGGAAG CCACCAACGC
    AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCAA GTAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849678.1
    CDS768..5099
    Translation

    Target ORF information:

    RefSeq Version XM_024993910.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAAGT TTTCTAGTCA TAGATCTAGC
    GTAGGTAGCT CCTCAGATGG TTCCATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGTAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGCGGGCGA
    GTGGACCCGC AACCAGCATT GCCTCACCGA AGGGAAGGAA TGACAGATGA GGAGACATTG
    GTGCCCTACA GCAAGCCCAA TTTCCCATCT CCAGGGGGTC ACAGCTCGTC AGGAACGGCT
    TCTTCTAAGG GATCCACTGG ACCTAGGAAA GCCGATGCTT TGAGGGGAAG CCACCAACGC
    AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCAA GTAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219058
    Clone ID Related Accession (Same CDS sequence) XM_024993915.1
    Accession Version XM_024993915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGAGGA GACATTGGTG
    CCCTACAGCA AGCCCAATTT CCCATCTCCA GGGGGTCACA GCTCGTCAGG AACGGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAGCC GATGCTTTGA GGGGAAGCCA CCAACGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCAAGTA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849683.1
    CDS699..4847
    Translation

    Target ORF information:

    RefSeq Version XM_024993915.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGAGGA GACATTGGTG
    CCCTACAGCA AGCCCAATTT CCCATCTCCA GGGGGTCACA GCTCGTCAGG AACGGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAGCC GATGCTTTGA GGGGAAGCCA CCAACGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCAAGTA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219059
    Clone ID Related Accession (Same CDS sequence) XM_024993918.1
    Accession Version XM_024993918.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGAGGA GACATTGGTG
    CCCTACAGCA AGCCCAATTT CCCATCTCCA GGGGGTCACA GCTCGTCAGG AACGGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAGCC GATGCTTTGA GGGGAAGCCA CCAACGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCAAGTA GTCAAGGACA GTTTACAGAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849686.1
    CDS699..4841
    Translation

    Target ORF information:

    RefSeq Version XM_024993918.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGAGGA GACATTGGTG
    CCCTACAGCA AGCCCAATTT CCCATCTCCA GGGGGTCACA GCTCGTCAGG AACGGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAGCC GATGCTTTGA GGGGAAGCCA CCAACGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCAAGTA GTCAAGGACA GTTTACAGAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219060
    Clone ID Related Accession (Same CDS sequence) XM_024993922.1
    Accession Version XM_024993922.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGCTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849690.1
    CDS698..4477
    Translation

    Target ORF information:

    RefSeq Version XM_024993922.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993922.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGCTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87636
    Clone ID Related Accession (Same CDS sequence) XM_015468542.1 , XM_015461935.1
    Accession Version XM_015468542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGCTG ATAGCAGCCA TTTAGTGCAG CTGAGAGAGA CAGTGCACTT CCTGGGAGGT 
    AGAGACAAGG ACCTGAGCTT CAGGCTGGCT GGGATTACAT CGCCCATCGT GAGAGTTTTC
    TTTTACTGCA ATTATTTTTC TTCAGTGTTA CGAGATGACT TCCGGCAAAA TCCCACAGAT
    GTTGTAGTGG CAGCCGGAGA GCCTGCAATC CTGGAGTGCC AGCCTCCCCG GGGACACCCA
    GAACCTACTA TCTACTGGAA AAAAGACAAA ATTCGAATTG ATGACAAGGA AGAAAGAATA
    AGTATCCGTG GTGGAAAACT AATGATCTCC AATACCAGAA AGAGTGATGC CGGGATGTAT
    ACGTGTGTGG GCACCAACAT GGTGGGGGAG AGAGACAGTG ATCCAGCAGA GCTGACTGTC
    TTTGAACGAC CCACATTTCT CAGGAGGCCA ATTAACCAAG TGGTACTGGA GGAAGAAGCT
    GTAGAGTTCC GTTGTCAGGT CCAGGGAGAT CCTCAGCCAA CTGTGCGGTG GAAAAAGGAT
    GATGCAGACT TGCCGAGAGG AAGGTATGAC ATCAAAGATG ATTACACACT GAGAATTAAA
    AAGGCAATGA GTACAGATGA AGGCACCTAT ATGTGTATTG CGGAGAATCG GGTTGGAAAA
    GTAGAAGCTT CTGCTACCCT CACAGTCCGA GCTCGCCCTG TGGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC AGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CCTGCTCTTC
    CCAAACCAAC CCCAACAGCC CAACAGTAGG TGTTCTGTGT CACCAACTGG GGACCTTACC
    ATTACCAACA TTCAGCGTTC GGACGCGGGT TACTACATCT GTCAGGCCCT CACCGTGGCA
    GGAAGTATTT TAGCAAAAGC TCAGCTGGAA GTGACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAG ACGTTAGCAG TAGATGGCAC AGCATTACTG
    AAATGTAAAG CTACTGGTGA CCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATCCGAG AGCAACTATA CAAGAACAAG GAACCTTACA GATTAAGAAC
    TTAAGGATTT CTGATACTGG CACTTACACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCCTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAGTTATGAT
    TTAAATGACC TACCTGGACC ACCTTCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAATCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA CATAAAGACC
    ACCCTCTATA CAGTGAGAGG ACTACGGCCC AATACAATCT ATTTATTCAT GGTCAGAGCA
    ATCAACCCCC AAGGTCTCAG TGACCCGAGT GCTATGTCAG ATCCTGTGCG CACCCAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTCC AGAAAGAACT CGGAGACGTC
    CTTGTCCGTC TTCATACTCC AGTCGTACTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATTCAAGGC TACCGAGTGA TGTACCGTCA GACTTCAGGC
    CTGCAGGCTA CAACAGCGTG GCAGAATTTA GATGCCAAAG TCCCAACTGA GAGAAGTGCT
    GTCTTAGTCA ATCTGAAAAA GGGGGTGACT TACGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACT GTCGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TTATCCAGGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAC ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTAATCA TTGGTGGATT ATTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCGGCTAGT
    ACCAGTGCAG GAGTTGGAGT AAAAAGTGAG CCACAGCCAA TAATAATTGG GGGACGAAAT
    GAAGTTGTCA TTACCGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCTT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATCCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAATTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGCC CAGGTCTTCT AAATGCTGGT
    GATCCCAGTT ATCCATGGCT TGCTGACTCA TGGCCAGCCA CGAGCTTGCC AGTAAACAAT
    AGCAGTAGTG GCCCAAATGA GATTGCAAAC TTTGGGCGTG GAGATGTGCT GCCACCGGTT
    CCAGGCCAAG GGGATAAAAC GGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAATAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATATTGCA TTCCAGTAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAGTGGA AAAGCTCAAT TCAGCAAAAA ACAGACCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACCTGGGCCA ATGTTCCACT ACCTCCTCCC
    CCAGTCCAGC CTCTTCCGGG TACAGAGCTG GAACACTATG CAGTGGAACA GCAAGAAAAT
    GGCTACGACA GTGATAGCTG GTGCCCACCA TTACCAGTTC AAACTTACTT ACACCAGGGT
    ATGGAGGATG AACTGGAAGA AGATGACGAT CGTGTTCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCCC CTGCTATTTC CTTTGGCCAG CAGTCCACGG CAACTCTGAC TCCCTCCCCA
    CGGGAAGAGA TGCAGCCCAT GCTGCAAGCT CACCTGGACG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAGCAAAC ATGGCATGTT CAAAGCAATA ATCAACCTCC GCAGCCCCCA
    GTTCCACCGT TAGGTTACAT GTCTGGAGCC TTGATTTCCG ATTTAGAAAC AGATGTTCCA
    GACGACGATG CTGATGATGA AGAGGAAGCT TTAGAAATCC CCAGACCCCT GCGAGCGCTA
    GAGCAGACAC CTGGATCCAG TACGGACAAT CTAGAGAGCT CTGTGACAGG TTCAATGGTA
    AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAAGTTTT CTAGTCATAG ATCTAGCGTA
    GGTAGCTCCT CAGATGGTTC CATCTTTGCC AGCGGCAGTT TTGCACAAGC ACTGGTGGCA
    GCAGCAGATA AAGCTGGTTT TAGGCTGGAT GGAACCAGCC TTACAAGAAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGATCT TCCACCACCA
    CCAGATCCCC CTCCAGGTCA GGGTTTAAGG CAGCAGATAG GCTCGAGCCA GCATGCTGGT
    AACGTGGAAA ATTCAACAGA GAGAAAAGGA AGCTCTCTGG AGAGACAGCA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC TTTGGATTGT CCAGCTAAAA CATCCCTAGA GTGGCAGCGA
    CAAACCCAGG AATGGATAAA CTCCACTGAA CGCCCAGAAG ACATACGGAA AGCCCTCCAC
    AAACAAGGTG TCGGACCAGA GGAGACATTG GTGCCCTACA GCAAGCCCAA TTTCCCATCT
    CCAGGGGGTC ACAGCTCGTC AGGAACGGCT TCTTCTAAGG GATCCACTGG ACCTAGGAAA
    GCCGATGCTT TGAGGGGAAG CCACCAACGC AATGCCAGCG ACCTTCTTGA CATAGGATAT
    ATGGGCTCAA GTAGTCAAGG ACAGTTTACA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324028.1
    CDS1..4356
    Translation

    Target ORF information:

    RefSeq Version XM_015468542.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGCTG ATAGCAGCCA TTTAGTGCAG CTGAGAGAGA CAGTGCACTT CCTGGGAGGT 
    AGAGACAAGG ACCTGAGCTT CAGGCTGGCT GGGATTACAT CGCCCATCGT GAGAGTTTTC
    TTTTACTGCA ATTATTTTTC TTCAGTGTTA CGAGATGACT TCCGGCAAAA TCCCACAGAT
    GTTGTAGTGG CAGCCGGAGA GCCTGCAATC CTGGAGTGCC AGCCTCCCCG GGGACACCCA
    GAACCTACTA TCTACTGGAA AAAAGACAAA ATTCGAATTG ATGACAAGGA AGAAAGAATA
    AGTATCCGTG GTGGAAAACT AATGATCTCC AATACCAGAA AGAGTGATGC CGGGATGTAT
    ACGTGTGTGG GCACCAACAT GGTGGGGGAG AGAGACAGTG ATCCAGCAGA GCTGACTGTC
    TTTGAACGAC CCACATTTCT CAGGAGGCCA ATTAACCAAG TGGTACTGGA GGAAGAAGCT
    GTAGAGTTCC GTTGTCAGGT CCAGGGAGAT CCTCAGCCAA CTGTGCGGTG GAAAAAGGAT
    GATGCAGACT TGCCGAGAGG AAGGTATGAC ATCAAAGATG ATTACACACT GAGAATTAAA
    AAGGCAATGA GTACAGATGA AGGCACCTAT ATGTGTATTG CGGAGAATCG GGTTGGAAAA
    GTAGAAGCTT CTGCTACCCT CACAGTCCGA GCTCGCCCTG TGGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC AGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CCTGCTCTTC
    CCAAACCAAC CCCAACAGCC CAACAGTAGG TGTTCTGTGT CACCAACTGG GGACCTTACC
    ATTACCAACA TTCAGCGTTC GGACGCGGGT TACTACATCT GTCAGGCCCT CACCGTGGCA
    GGAAGTATTT TAGCAAAAGC TCAGCTGGAA GTGACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAG ACGTTAGCAG TAGATGGCAC AGCATTACTG
    AAATGTAAAG CTACTGGTGA CCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATCCGAG AGCAACTATA CAAGAACAAG GAACCTTACA GATTAAGAAC
    TTAAGGATTT CTGATACTGG CACTTACACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCCTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAGTTATGAT
    TTAAATGACC TACCTGGACC ACCTTCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAATCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA CATAAAGACC
    ACCCTCTATA CAGTGAGAGG ACTACGGCCC AATACAATCT ATTTATTCAT GGTCAGAGCA
    ATCAACCCCC AAGGTCTCAG TGACCCGAGT GCTATGTCAG ATCCTGTGCG CACCCAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTCC AGAAAGAACT CGGAGACGTC
    CTTGTCCGTC TTCATACTCC AGTCGTACTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATTCAAGGC TACCGAGTGA TGTACCGTCA GACTTCAGGC
    CTGCAGGCTA CAACAGCGTG GCAGAATTTA GATGCCAAAG TCCCAACTGA GAGAAGTGCT
    GTCTTAGTCA ATCTGAAAAA GGGGGTGACT TACGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACT GTCGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TTATCCAGGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAC ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTAATCA TTGGTGGATT ATTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCGGCTAGT
    ACCAGTGCAG GAGTTGGAGT AAAAAGTGAG CCACAGCCAA TAATAATTGG GGGACGAAAT
    GAAGTTGTCA TTACCGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCTT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATCCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAATTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGCC CAGGTCTTCT AAATGCTGGT
    GATCCCAGTT ATCCATGGCT TGCTGACTCA TGGCCAGCCA CGAGCTTGCC AGTAAACAAT
    AGCAGTAGTG GCCCAAATGA GATTGCAAAC TTTGGGCGTG GAGATGTGCT GCCACCGGTT
    CCAGGCCAAG GGGATAAAAC GGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAATAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATATTGCA TTCCAGTAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAGTGGA AAAGCTCAAT TCAGCAAAAA ACAGACCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACCTGGGCCA ATGTTCCACT ACCTCCTCCC
    CCAGTCCAGC CTCTTCCGGG TACAGAGCTG GAACACTATG CAGTGGAACA GCAAGAAAAT
    GGCTACGACA GTGATAGCTG GTGCCCACCA TTACCAGTTC AAACTTACTT ACACCAGGGT
    ATGGAGGATG AACTGGAAGA AGATGACGAT CGTGTTCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCCC CTGCTATTTC CTTTGGCCAG CAGTCCACGG CAACTCTGAC TCCCTCCCCA
    CGGGAAGAGA TGCAGCCCAT GCTGCAAGCT CACCTGGACG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAGCAAAC ATGGCATGTT CAAAGCAATA ATCAACCTCC GCAGCCCCCA
    GTTCCACCGT TAGGTTACAT GTCTGGAGCC TTGATTTCCG ATTTAGAAAC AGATGTTCCA
    GACGACGATG CTGATGATGA AGAGGAAGCT TTAGAAATCC CCAGACCCCT GCGAGCGCTA
    GAGCAGACAC CTGGATCCAG TACGGACAAT CTAGAGAGCT CTGTGACAGG TTCAATGGTA
    AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAAGTTTT CTAGTCATAG ATCTAGCGTA
    GGTAGCTCCT CAGATGGTTC CATCTTTGCC AGCGGCAGTT TTGCACAAGC ACTGGTGGCA
    GCAGCAGATA AAGCTGGTTT TAGGCTGGAT GGAACCAGCC TTACAAGAAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGATCT TCCACCACCA
    CCAGATCCCC CTCCAGGTCA GGGTTTAAGG CAGCAGATAG GCTCGAGCCA GCATGCTGGT
    AACGTGGAAA ATTCAACAGA GAGAAAAGGA AGCTCTCTGG AGAGACAGCA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC TTTGGATTGT CCAGCTAAAA CATCCCTAGA GTGGCAGCGA
    CAAACCCAGG AATGGATAAA CTCCACTGAA CGCCCAGAAG ACATACGGAA AGCCCTCCAC
    AAACAAGGTG TCGGACCAGA GGAGACATTG GTGCCCTACA GCAAGCCCAA TTTCCCATCT
    CCAGGGGGTC ACAGCTCGTC AGGAACGGCT TCTTCTAAGG GATCCACTGG ACCTAGGAAA
    GCCGATGCTT TGAGGGGAAG CCACCAACGC AATGCCAGCG ACCTTCTTGA CATAGGATAT
    ATGGGCTCAA GTAGTCAAGG ACAGTTTACA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87636
    Clone ID Related Accession (Same CDS sequence) XM_015468542.1 , XM_015461935.1
    Accession Version XM_015461935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644501.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGCTG ATAGCAGCCA TTTAGTGCAG CTGAGAGAGA CAGTGCACTT CCTGGGAGGT 
    AGAGACAAGG ACCTGAGCTT CAGGCTGGCT GGGATTACAT CGCCCATCGT GAGAGTTTTC
    TTTTACTGCA ATTATTTTTC TTCAGTGTTA CGAGATGACT TCCGGCAAAA TCCCACAGAT
    GTTGTAGTGG CAGCCGGAGA GCCTGCAATC CTGGAGTGCC AGCCTCCCCG GGGACACCCA
    GAACCTACTA TCTACTGGAA AAAAGACAAA ATTCGAATTG ATGACAAGGA AGAAAGAATA
    AGTATCCGTG GTGGAAAACT AATGATCTCC AATACCAGAA AGAGTGATGC CGGGATGTAT
    ACGTGTGTGG GCACCAACAT GGTGGGGGAG AGAGACAGTG ATCCAGCAGA GCTGACTGTC
    TTTGAACGAC CCACATTTCT CAGGAGGCCA ATTAACCAAG TGGTACTGGA GGAAGAAGCT
    GTAGAGTTCC GTTGTCAGGT CCAGGGAGAT CCTCAGCCAA CTGTGCGGTG GAAAAAGGAT
    GATGCAGACT TGCCGAGAGG AAGGTATGAC ATCAAAGATG ATTACACACT GAGAATTAAA
    AAGGCAATGA GTACAGATGA AGGCACCTAT ATGTGTATTG CGGAGAATCG GGTTGGAAAA
    GTAGAAGCTT CTGCTACCCT CACAGTCCGA GCTCGCCCTG TGGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC AGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CCTGCTCTTC
    CCAAACCAAC CCCAACAGCC CAACAGTAGG TGTTCTGTGT CACCAACTGG GGACCTTACC
    ATTACCAACA TTCAGCGTTC GGACGCGGGT TACTACATCT GTCAGGCCCT CACCGTGGCA
    GGAAGTATTT TAGCAAAAGC TCAGCTGGAA GTGACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAG ACGTTAGCAG TAGATGGCAC AGCATTACTG
    AAATGTAAAG CTACTGGTGA CCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATCCGAG AGCAACTATA CAAGAACAAG GAACCTTACA GATTAAGAAC
    TTAAGGATTT CTGATACTGG CACTTACACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCCTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAGTTATGAT
    TTAAATGACC TACCTGGACC ACCTTCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAATCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA CATAAAGACC
    ACCCTCTATA CAGTGAGAGG ACTACGGCCC AATACAATCT ATTTATTCAT GGTCAGAGCA
    ATCAACCCCC AAGGTCTCAG TGACCCGAGT GCTATGTCAG ATCCTGTGCG CACCCAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTCC AGAAAGAACT CGGAGACGTC
    CTTGTCCGTC TTCATACTCC AGTCGTACTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATTCAAGGC TACCGAGTGA TGTACCGTCA GACTTCAGGC
    CTGCAGGCTA CAACAGCGTG GCAGAATTTA GATGCCAAAG TCCCAACTGA GAGAAGTGCT
    GTCTTAGTCA ATCTGAAAAA GGGGGTGACT TACGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACT GTCGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TTATCCAGGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAC ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTAATCA TTGGTGGATT ATTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCGGCTAGT
    ACCAGTGCAG GAGTTGGAGT AAAAAGTGAG CCACAGCCAA TAATAATTGG GGGACGAAAT
    GAAGTTGTCA TTACCGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCTT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATCCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAATTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGCC CAGGTCTTCT AAATGCTGGT
    GATCCCAGTT ATCCATGGCT TGCTGACTCA TGGCCAGCCA CGAGCTTGCC AGTAAACAAT
    AGCAGTAGTG GCCCAAATGA GATTGCAAAC TTTGGGCGTG GAGATGTGCT GCCACCGGTT
    CCAGGCCAAG GGGATAAAAC GGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAATAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATATTGCA TTCCAGTAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAGTGGA AAAGCTCAAT TCAGCAAAAA ACAGACCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACCTGGGCCA ATGTTCCACT ACCTCCTCCC
    CCAGTCCAGC CTCTTCCGGG TACAGAGCTG GAACACTATG CAGTGGAACA GCAAGAAAAT
    GGCTACGACA GTGATAGCTG GTGCCCACCA TTACCAGTTC AAACTTACTT ACACCAGGGT
    ATGGAGGATG AACTGGAAGA AGATGACGAT CGTGTTCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCCC CTGCTATTTC CTTTGGCCAG CAGTCCACGG CAACTCTGAC TCCCTCCCCA
    CGGGAAGAGA TGCAGCCCAT GCTGCAAGCT CACCTGGACG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAGCAAAC ATGGCATGTT CAAAGCAATA ATCAACCTCC GCAGCCCCCA
    GTTCCACCGT TAGGTTACAT GTCTGGAGCC TTGATTTCCG ATTTAGAAAC AGATGTTCCA
    GACGACGATG CTGATGATGA AGAGGAAGCT TTAGAAATCC CCAGACCCCT GCGAGCGCTA
    GAGCAGACAC CTGGATCCAG TACGGACAAT CTAGAGAGCT CTGTGACAGG TTCAATGGTA
    AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAAGTTTT CTAGTCATAG ATCTAGCGTA
    GGTAGCTCCT CAGATGGTTC CATCTTTGCC AGCGGCAGTT TTGCACAAGC ACTGGTGGCA
    GCAGCAGATA AAGCTGGTTT TAGGCTGGAT GGAACCAGCC TTACAAGAAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGATCT TCCACCACCA
    CCAGATCCCC CTCCAGGTCA GGGTTTAAGG CAGCAGATAG GCTCGAGCCA GCATGCTGGT
    AACGTGGAAA ATTCAACAGA GAGAAAAGGA AGCTCTCTGG AGAGACAGCA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC TTTGGATTGT CCAGCTAAAA CATCCCTAGA GTGGCAGCGA
    CAAACCCAGG AATGGATAAA CTCCACTGAA CGCCCAGAAG ACATACGGAA AGCCCTCCAC
    AAACAAGGTG TCGGACCAGA GGAGACATTG GTGCCCTACA GCAAGCCCAA TTTCCCATCT
    CCAGGGGGTC ACAGCTCGTC AGGAACGGCT TCTTCTAAGG GATCCACTGG ACCTAGGAAA
    GCCGATGCTT TGAGGGGAAG CCACCAACGC AATGCCAGCG ACCTTCTTGA CATAGGATAT
    ATGGGCTCAA GTAGTCAAGG ACAGTTTACA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317421.1
    CDS1..4356
    Translation

    Target ORF information:

    RefSeq Version XM_015461935.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGCTG ATAGCAGCCA TTTAGTGCAG CTGAGAGAGA CAGTGCACTT CCTGGGAGGT 
    AGAGACAAGG ACCTGAGCTT CAGGCTGGCT GGGATTACAT CGCCCATCGT GAGAGTTTTC
    TTTTACTGCA ATTATTTTTC TTCAGTGTTA CGAGATGACT TCCGGCAAAA TCCCACAGAT
    GTTGTAGTGG CAGCCGGAGA GCCTGCAATC CTGGAGTGCC AGCCTCCCCG GGGACACCCA
    GAACCTACTA TCTACTGGAA AAAAGACAAA ATTCGAATTG ATGACAAGGA AGAAAGAATA
    AGTATCCGTG GTGGAAAACT AATGATCTCC AATACCAGAA AGAGTGATGC CGGGATGTAT
    ACGTGTGTGG GCACCAACAT GGTGGGGGAG AGAGACAGTG ATCCAGCAGA GCTGACTGTC
    TTTGAACGAC CCACATTTCT CAGGAGGCCA ATTAACCAAG TGGTACTGGA GGAAGAAGCT
    GTAGAGTTCC GTTGTCAGGT CCAGGGAGAT CCTCAGCCAA CTGTGCGGTG GAAAAAGGAT
    GATGCAGACT TGCCGAGAGG AAGGTATGAC ATCAAAGATG ATTACACACT GAGAATTAAA
    AAGGCAATGA GTACAGATGA AGGCACCTAT ATGTGTATTG CGGAGAATCG GGTTGGAAAA
    GTAGAAGCTT CTGCTACCCT CACAGTCCGA GCTCGCCCTG TGGCTCCTCC ACAGTTTGTG
    GTTAGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC AGTTTTTTGG CAAAAAGAAG GCAGCCAGAA CCTGCTCTTC
    CCAAACCAAC CCCAACAGCC CAACAGTAGG TGTTCTGTGT CACCAACTGG GGACCTTACC
    ATTACCAACA TTCAGCGTTC GGACGCGGGT TACTACATCT GTCAGGCCCT CACCGTGGCA
    GGAAGTATTT TAGCAAAAGC TCAGCTGGAA GTGACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAG ACGTTAGCAG TAGATGGCAC AGCATTACTG
    AAATGTAAAG CTACTGGTGA CCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCTGGGTA GAGATCCGAG AGCAACTATA CAAGAACAAG GAACCTTACA GATTAAGAAC
    TTAAGGATTT CTGATACTGG CACTTACACT TGTGTGGCTA CAAGTTCAAG TGGGGAGACT
    TCCTGGAGTG CTGTGCTGGA TGTGACAGAA TCTGGAGCAA CAATCAGTAA AAGTTATGAT
    TTAAATGACC TACCTGGACC ACCTTCCAAA CCACAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAACC AGGTACCCCT GGAATCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA CATAAAGACC
    ACCCTCTATA CAGTGAGAGG ACTACGGCCC AATACAATCT ATTTATTCAT GGTCAGAGCA
    ATCAACCCCC AAGGTCTCAG TGACCCGAGT GCTATGTCAG ATCCTGTGCG CACCCAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTCC AGAAAGAACT CGGAGACGTC
    CTTGTCCGTC TTCATACTCC AGTCGTACTG ACTCCCACCA CTGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATTCAAGGC TACCGAGTGA TGTACCGTCA GACTTCAGGC
    CTGCAGGCTA CAACAGCGTG GCAGAATTTA GATGCCAAAG TCCCAACTGA GAGAAGTGCT
    GTCTTAGTCA ATCTGAAAAA GGGGGTGACT TACGAAATTA AAGTTCGACC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACAGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCTCCAC AATCTGTCAC TGTGCTGACT GTCGGAAGCC ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAT CAGAATGGAA TTATCCAGGA ATACAAGATC
    TGGTGTCTGG GGAATGAAAC ACGATTCCAC ATCAACAAAA CTGTGGATGC AGCCATTCGA
    TCTGTAATCA TTGGTGGATT ATTCCCAGGT ATTCAGTACC GGGTAGAGGT TGCGGCTAGT
    ACCAGTGCAG GAGTTGGAGT AAAAAGTGAG CCACAGCCAA TAATAATTGG GGGACGAAAT
    GAAGTTGTCA TTACCGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTTGTGAAG
    CAACCAGCTT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATCCTGAT GGGTTTTAGC
    ATCTGGTTGT ACTGGCGAAG AAAGAAGAGA AAGGGACTCA GTAATTATGC TGTTACATTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGCC CAGGTCTTCT AAATGCTGGT
    GATCCCAGTT ATCCATGGCT TGCTGACTCA TGGCCAGCCA CGAGCTTGCC AGTAAACAAT
    AGCAGTAGTG GCCCAAATGA GATTGCAAAC TTTGGGCGTG GAGATGTGCT GCCACCGGTT
    CCAGGCCAAG GGGATAAAAC GGCAACGATG CTCTCAGATG GAGCCATTTA TAGCAGCATT
    GACTTCACGA CCAAAACCAC TTACAATAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATATTGCA TTCCAGTAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAGTGGA AAAGCTCAAT TCAGCAAAAA ACAGACCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCG ACCTGGGCCA ATGTTCCACT ACCTCCTCCC
    CCAGTCCAGC CTCTTCCGGG TACAGAGCTG GAACACTATG CAGTGGAACA GCAAGAAAAT
    GGCTACGACA GTGATAGCTG GTGCCCACCA TTACCAGTTC AAACTTACTT ACACCAGGGT
    ATGGAGGATG AACTGGAAGA AGATGACGAT CGTGTTCCAA CACCTCCTGT CCGAGGCGTG
    GCTTCTTCCC CTGCTATTTC CTTTGGCCAG CAGTCCACGG CAACTCTGAC TCCCTCCCCA
    CGGGAAGAGA TGCAGCCCAT GCTGCAAGCT CACCTGGACG AGTTGACAAG GGCCTATCAG
    TTTGATATAG CAAAGCAAAC ATGGCATGTT CAAAGCAATA ATCAACCTCC GCAGCCCCCA
    GTTCCACCGT TAGGTTACAT GTCTGGAGCC TTGATTTCCG ATTTAGAAAC AGATGTTCCA
    GACGACGATG CTGATGATGA AGAGGAAGCT TTAGAAATCC CCAGACCCCT GCGAGCGCTA
    GAGCAGACAC CTGGATCCAG TACGGACAAT CTAGAGAGCT CTGTGACAGG TTCAATGGTA
    AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAAGTTTT CTAGTCATAG ATCTAGCGTA
    GGTAGCTCCT CAGATGGTTC CATCTTTGCC AGCGGCAGTT TTGCACAAGC ACTGGTGGCA
    GCAGCAGATA AAGCTGGTTT TAGGCTGGAT GGAACCAGCC TTACAAGAAC AGGTAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCAACC AGCCCATTTT CTACGGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG CGGGCGAGTG
    GACCCGCAAC CAGCATTGCC TCACCGAAGG GAAGGAATGA CAGATGATCT TCCACCACCA
    CCAGATCCCC CTCCAGGTCA GGGTTTAAGG CAGCAGATAG GCTCGAGCCA GCATGCTGGT
    AACGTGGAAA ATTCAACAGA GAGAAAAGGA AGCTCTCTGG AGAGACAGCA AGCAGCCAAC
    TTAGAAGACA CAAAGAGCTC TTTGGATTGT CCAGCTAAAA CATCCCTAGA GTGGCAGCGA
    CAAACCCAGG AATGGATAAA CTCCACTGAA CGCCCAGAAG ACATACGGAA AGCCCTCCAC
    AAACAAGGTG TCGGACCAGA GGAGACATTG GTGCCCTACA GCAAGCCCAA TTTCCCATCT
    CCAGGGGGTC ACAGCTCGTC AGGAACGGCT TCTTCTAAGG GATCCACTGG ACCTAGGAAA
    GCCGATGCTT TGAGGGGAAG CCACCAACGC AATGCCAGCG ACCTTCTTGA CATAGGATAT
    ATGGGCTCAA GTAGTCAAGG ACAGTTTACA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219054
    Clone ID Related Accession (Same CDS sequence) XM_024993901.1
    Accession Version XM_024993901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAAGT TTTCTAGTCA TAGATCTAGC
    GTAGGTAGCT CCTCAGATGG TTCCATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGTAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGCGGGCGA
    GTGGACCCGC AACCAGCATT GCCTCACCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAGA TAGGCTCGAG CCAGCATGCT
    GGTAACGTGG AAAATTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGAGACA GCAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCTTTGGAT TGTCCAGCTA AAACATCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAACTCCACT GAACGCCCAG AAGACATACG GAAAGCCCTC
    CACAAACAAG GTGTCGGACC AGAGGAGACA TTGGTGCCCT ACAGCAAGCC CAATTTCCCA
    TCTCCAGGGG GTCACAGCTC GTCAGGAACG GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAGCCGATG CTTTGAGGGG AAGCCACCAA CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CAAGTAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849669.1
    CDS700..5304
    Translation

    Target ORF information:

    RefSeq Version XM_024993901.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAAGT TTTCTAGTCA TAGATCTAGC
    GTAGGTAGCT CCTCAGATGG TTCCATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGTAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGCGGGCGA
    GTGGACCCGC AACCAGCATT GCCTCACCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAGA TAGGCTCGAG CCAGCATGCT
    GGTAACGTGG AAAATTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGAGACA GCAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCTTTGGAT TGTCCAGCTA AAACATCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAACTCCACT GAACGCCCAG AAGACATACG GAAAGCCCTC
    CACAAACAAG GTGTCGGACC AGAGGAGACA TTGGTGCCCT ACAGCAAGCC CAATTTCCCA
    TCTCCAGGGG GTCACAGCTC GTCAGGAACG GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAGCCGATG CTTTGAGGGG AAGCCACCAA CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CAAGTAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219055
    Clone ID Related Accession (Same CDS sequence) XM_024993905.1
    Accession Version XM_024993905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAAGT TTTCTAGTCA TAGATCTAGC
    GTAGGTAGCT CCTCAGATGG TTCCATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGTAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGCGGGCGA
    GTGGACCCGC AACCAGCATT GCCTCACCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAGA TAGGCTCGAG CCAGCATGCT
    GGTAACGTGG AAAATTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGAGACA GCAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCTTTGGAT TGTCCAGCTA AAACATCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAACTCCACT GAACGCCCAG AAGACATACG GAAAGCCCTC
    CACAAACAAG GTGTCGGACC AGAGGAGACA TTGGTGCCCT ACAGCAAGCC CAATTTCCCA
    TCTCCAGGGG GTCACAGCTC GTCAGGAACG GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAGCCGATG CTTTGAGGGG AAGCCACCAA CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CAAGTAGTCA AGGACAGTTT ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849673.1
    CDS535..5133
    Translation

    Target ORF information:

    RefSeq Version XM_024993905.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTCGGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCTCGTC TTCGCCAGGA AGACTTTCCC CCAAGGATCG TGGAGCACCC TTCCGATGTC
    ATTGTCTCTA AGGGAGAGCC CACTACTTTG AACTGTAAGG CCGAGGGCCG GCCAACGCCC
    ACCATCGAGT GGTACAAGGA TGGTGAGCGG GTGGAGACTG ACAAGGATGA TCCCCGGTCC
    CATAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATTGTGCA TGGGCGCAGA
    AGTAAACCAG ATGAAGGAAG CTATGTGTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CATCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGGCA AAATCCCACA
    GATGTTGTAG TGGCAGCCGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCTA CTATCTACTG GAAAAAAGAC AAAATTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTAATGATC TCCAATACCA GAAAGAGTGA TGCCGGGATG
    TATACGTGTG TGGGCACCAA CATGGTGGGG GAGAGAGACA GTGATCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AAGTGGTACT GGAGGAAGAA
    GCTGTAGAGT TCCGTTGTCA GGTCCAGGGA GATCCTCAGC CAACTGTGCG GTGGAAAAAG
    GATGATGCAG ACTTGCCGAG AGGAAGGTAT GACATCAAAG ATGATTACAC ACTGAGAATT
    AAAAAGGCAA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCGGAGAA TCGGGTTGGA
    AAAGTAGAAG CTTCTGCTAC CCTCACAGTC CGAGCTCGCC CTGTGGCTCC TCCACAGTTT
    GTGGTTAGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCAGTTTTT TGGCAAAAAG AAGGCAGCCA GAACCTGCTC
    TTCCCAAACC AACCCCAACA GCCCAACAGT AGGTGTTCTG TGTCACCAAC TGGGGACCTT
    ACCATTACCA ACATTCAGCG TTCGGACGCG GGTTACTACA TCTGTCAGGC CCTCACCGTG
    GCAGGAAGTA TTTTAGCAAA AGCTCAGCTG GAAGTGACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAGACGTTAG CAGTAGATGG CACAGCATTA
    CTGAAATGTA AAGCTACTGG TGACCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCTGG GTAGAGATCC GAGAGCAACT ATACAAGAAC AAGGAACCTT ACAGATTAAG
    AACTTAAGGA TTTCTGATAC TGGCACTTAC ACTTGTGTGG CTACAAGTTC AAGTGGGGAG
    ACTTCCTGGA GTGCTGTGCT GGATGTGACA GAATCTGGAG CAACAATCAG TAAAAGTTAT
    GATTTAAATG ACCTACCTGG ACCACCTTCC AAACCACAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA ACCAGGTACC CCTGGAATCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCACATAAAG
    ACCACCCTCT ATACAGTGAG AGGACTACGG CCCAATACAA TCTATTTATT CATGGTCAGA
    GCAATCAACC CCCAAGGTCT CAGTGACCCG AGTGCTATGT CAGATCCTGT GCGCACCCAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TCCAGAAAGA ACTCGGAGAC
    GTCCTTGTCC GTCTTCATAC TCCAGTCGTA CTGACTCCCA CCACTGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATTCAA GGCTACCGAG TGATGTACCG TCAGACTTCA
    GGCCTGCAGG CTACAACAGC GTGGCAGAAT TTAGATGCCA AAGTCCCAAC TGAGAGAAGT
    GCTGTCTTAG TCAATCTGAA AAAGGGGGTG ACTTACGAAA TTAAAGTTCG ACCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACAGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCTC CACAATCTGT CACTGTGCTG ACTGTCGGAA GCCACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CATCAGAATG GAATTATCCA GGAATACAAG
    ATCTGGTGTC TGGGGAATGA AACACGATTC CACATCAACA AAACTGTGGA TGCAGCCATT
    CGATCTGTAA TCATTGGTGG ATTATTCCCA GGTATTCAGT ACCGGGTAGA GGTTGCGGCT
    AGTACCAGTG CAGGAGTTGG AGTAAAAAGT GAGCCACAGC CAATAATAAT TGGGGGACGA
    AATGAAGTTG TCATTACCGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTTGTG
    AAGCAACCAG CTTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATCCT GATGGGTTTT
    AGCATCTGGT TGTACTGGCG AAGAAAGAAG AGAAAGGGAC TCAGTAATTA TGCTGTTACA
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GCCCAGGTCT TCTAAATGCT
    GGTGATCCCA GTTATCCATG GCTTGCTGAC TCATGGCCAG CCACGAGCTT GCCAGTAAAC
    AATAGCAGTA GTGGCCCAAA TGAGATTGCA AACTTTGGGC GTGGAGATGT GCTGCCACCG
    GTTCCAGGCC AAGGGGATAA AACGGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGCAGC
    ATTGACTTCA CGACCAAAAC CACTTACAAT AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATATT GCATTCCAGT AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAGT GGAAAAGCTC AATTCAGCAA AAAACAGACC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCGACCTGGG CCAATGTTCC ACTACCTCCT
    CCCCCAGTCC AGCCTCTTCC GGGTACAGAG CTGGAACACT ATGCAGTGGA ACAGCAAGAA
    AATGGCTACG ACAGTGATAG CTGGTGCCCA CCATTACCAG TTCAAACTTA CTTACACCAG
    GGTATGGAGG ATGAACTGGA AGAAGATGAC GATCGTGTTC CAACACCTCC TGTCCGAGGC
    GTGGCTTCTT CCCCTGCTAT TTCCTTTGGC CAGCAGTCCA CGGCAACTCT GACTCCCTCC
    CCACGGGAAG AGATGCAGCC CATGCTGCAA GCTCACCTGG ACGAGTTGAC AAGGGCCTAT
    CAGTTTGATA TAGCAAAGCA AACATGGCAT GTTCAAAGCA ATAATCAACC TCCGCAGCCC
    CCAGTTCCAC CGTTAGGTTA CATGTCTGGA GCCTTGATTT CCGATTTAGA AACAGATGTT
    CCAGACGACG ATGCTGATGA TGAAGAGGAA GCTTTAGAAA TCCCCAGACC CCTGCGAGCG
    CTAGAGCAGA CACCTGGATC CAGTACGGAC AATCTAGAGA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAAGT TTTCTAGTCA TAGATCTAGC
    GTAGGTAGCT CCTCAGATGG TTCCATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGTAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCA ACCAGCCCAT TTTCTACGGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGCGGGCGA
    GTGGACCCGC AACCAGCATT GCCTCACCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCTCCAGG TCAGGGTTTA AGGCAGCAGA TAGGCTCGAG CCAGCATGCT
    GGTAACGTGG AAAATTCAAC AGAGAGAAAA GGAAGCTCTC TGGAGAGACA GCAAGCAGCC
    AACTTAGAAG ACACAAAGAG CTCTTTGGAT TGTCCAGCTA AAACATCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAACTCCACT GAACGCCCAG AAGACATACG GAAAGCCCTC
    CACAAACAAG GTGTCGGACC AGAGGAGACA TTGGTGCCCT ACAGCAAGCC CAATTTCCCA
    TCTCCAGGGG GTCACAGCTC GTCAGGAACG GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAGCCGATG CTTTGAGGGG AAGCCACCAA CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CAAGTAGTCA AGGACAGTTT ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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