POLA1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following POLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238447 XM_024988125.1
Latest version!
Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa89430 XM_015461765.1
Latest version!
Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa89431 XM_015461766.1
Latest version!
Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa40907 NM_001206065.1
Latest version!
Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa35737 XM_005228356.4
Latest version!
Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa35739 XM_005228359.4
Latest version!
Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa35740 XM_005228360.4
Latest version!
Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa35737 XM_005228356.3 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa35740 XM_005228360.3 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa35739 XM_005228359.3 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238447
    Clone ID Related Accession (Same CDS sequence) XM_024988125.1
    Accession Version XM_024988125.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGATAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843893.1
    CDS196..4368
    Translation

    Target ORF information:

    RefSeq Version XM_024988125.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988125.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGATAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89430
    Clone ID Related Accession (Same CDS sequence) XM_015461765.1
    Accession Version XM_015461765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGCAGGA GTGGATTTGG TGAAAAAAAT GCTACCTGTG GCCGAATGAT CTGTGATGTG
    GAAATTTCGG CGAAGGAACT GATTCGGTGT AAAAGCTACC ATTTATCTGA ACTTGTTCAA
    CAGATTTTGA AAACTGAAAG GATTGTAATC CCAATAGAAA CTATACGAAA CATGTACAGT
    GACTCTTCTC ATCTGTTGTA CCTGTTGGAG CACACCTGGA AAGATGCCAG GTTCATTTTG
    CAGATCATGT GTGAGCTAAA TGTTCTTCCA TTAGCATTGC AGATCACTAA CATCGCTGGG
    AACATTATGT CTAGGACACT GATGGGTGGA CGATCCGAGC GTAATGAGTT CTTACTACTT
    CATGCATTTT ATGAAAACAA CTATATTGTG CCTGACAAGC AGACTTTCAG AAAGCCTCAG
    CAGAAACTGG GAGATGAAGA TGAAGACATT GATGGAGACA CCAATAAATA CAAAAAAGGA
    CGTAAGAAAG CAGCTTATTC TGGAGGCTTG GTCTTGGACC CCAAAGTTGG TTTTTATGAT
    AAGTTCATTT TGCTTCTGGA CTTCAACAGT TTGTATCCTT CCATCATTCA GGAATTCAAC
    ATTTGTTTTA CAACAGTGCA AAGAGTTGCT TCAGAGGCAC AGAAAGTTGT AGAGGATGGA
    GAGCAAGAGC AGATCCCCGA GCTGCCAGAT CCAAGCTTGG AAATGGGCAT TTTGCCCAGA
    GAGATCCGAA AACTGGTAGA GCGGAGAAGA CAAGTAAAAC AGCTAATGAA ACAGCAAGAT
    TTAAATCCAG ACCTTCATCT TCAGTATGAC ATTCGACAGA AGGCTTTGAA GCTCACGGCA
    AACAGTATGT ACGGCTGCCT GGGATTCTCC TACAGCAGAT TTTACGCCAA ACCCCTGGCT
    GCTTTGGTGA CATACAAAGG AAGGGAGATT TTGATGCATA CGAAGGAGAT GGTTCAGAAG
    ATGAATCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATCAACAC CAATAGCACC
    AATCTGGAAG AAGTGTTTAA ATTGGGAAAC AAGGTGAAAA GTGAAGTGAA TAAGTTGTAC
    AAACTGCTTG AAATAGACAT TGATGGTATT TTCAAGTCCC TGCTACTGCT GAAGAAAAAA
    AAGTATGCTG CTCTGATTGT TGAGCCAACA TCAGATGGGA ATTACGTCAC GAAACAGGAG
    GTGAAAGGAT TAGATATAGT TAGAAGAGAC TGGTGTGATC TTGCTAAAGA CACTGGAAAC
    TTTGTCATTG GCCAGATTCT TTCGGATCAA AACCGGGACA CTATAGTGGA AAACATTCAG
    AAGAGGTTAA TAGAAATTGG AGAAAATGTG CTAAACGGCA GTGTCCCAGT GAGCCAGTTT
    GAAATTAATA AGGCGTTGAC CAAGGATCCC CAGGATTACC CTGATAAAAA AAGCCTACCT
    CATGTACATG TTGCCCTCTG GATAAATTCT CAAGGAGGAA GAAAGGTGAA AGCTGGAGAT
    ACTGTGTCCT ATGTCATCTG TCAGGATGGT TCCAATCTCA CTGCGGGTCA GAGGGCCTAT
    GCACCCGAGC AGCTGCAGAA GCAGGACAAT TTAAGCATTG ATACCCAGTA CTACCTGGCC
    CAGCAGATCC ACCCAGTCGT GGCTCGAATA TGTGAGCCAA TAGATGGAAT TGATGCTATC
    CTCATTGCAT CCTGGTTAGG ACTTGACCCC ACCCAATTTA GGGTTCATCA TTATCATAAA
    GATGAAGAGA ATGATGCTTT ACTTGGTGGC CCAGCACACC TCACGGATGA AGAGAAATAC
    AAGGACTGTG AAAGGTTCAA ATGCCCATGC CCTACATGTG GAACCGAGAA TATTTATGAT
    AGTGTCTTAG ATGGTTCGGG AACTGATATG GAGCCCAGCT TGTATCGTTG CAGTAACATC
    AATTGTAAGG CTTCTCCTCT GACCTTTATG GTACAGCTGA GCAACAAATT GATCATGGAC
    ATTAGACGTT GCATTAAAAA ATACTATGAA GGCTGGTTGA TTTGTGAAGA GCCCACATGT
    CGAAATCGAA CTCGGCACCT TCCCCTTCAG TTCTCTCGAA ATGGACCCCT TTGCCAAGTC
    TGCATGAAAG CTACACTTCG ACTAGAGTAT TCTGACAAGT CCCTGTACAC CCAGCTGTGC
    TTTTACCGGT ACATTTTTGA TGCGGAGTGT GCACTGGAGA AACTTATTAC CGATCATGAG
    AAAGATAAAT TGAAGAAGCA GTTTTTTACT CCCAAAGTTC TTCAGGACTA TAGAAAACTC
    AAGAACACAG CAGAGCAGTT CTTGTCCCAA AGTGGCTATT CCGAAGTGAA CCTCAGCAAA
    CTCTTCGCCG ATTGTGCTGT ATGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317251.1
    CDS175..4464
    Translation

    Target ORF information:

    RefSeq Version XM_015461765.1
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGCAGGA GTGGATTTGG TGAAAAAAAT GCTACCTGTG GCCGAATGAT CTGTGATGTG
    GAAATTTCGG CGAAGGAACT GATTCGGTGT AAAAGCTACC ATTTATCTGA ACTTGTTCAA
    CAGATTTTGA AAACTGAAAG GATTGTAATC CCAATAGAAA CTATACGAAA CATGTACAGT
    GACTCTTCTC ATCTGTTGTA CCTGTTGGAG CACACCTGGA AAGATGCCAG GTTCATTTTG
    CAGATCATGT GTGAGCTAAA TGTTCTTCCA TTAGCATTGC AGATCACTAA CATCGCTGGG
    AACATTATGT CTAGGACACT GATGGGTGGA CGATCCGAGC GTAATGAGTT CTTACTACTT
    CATGCATTTT ATGAAAACAA CTATATTGTG CCTGACAAGC AGACTTTCAG AAAGCCTCAG
    CAGAAACTGG GAGATGAAGA TGAAGACATT GATGGAGACA CCAATAAATA CAAAAAAGGA
    CGTAAGAAAG CAGCTTATTC TGGAGGCTTG GTCTTGGACC CCAAAGTTGG TTTTTATGAT
    AAGTTCATTT TGCTTCTGGA CTTCAACAGT TTGTATCCTT CCATCATTCA GGAATTCAAC
    ATTTGTTTTA CAACAGTGCA AAGAGTTGCT TCAGAGGCAC AGAAAGTTGT AGAGGATGGA
    GAGCAAGAGC AGATCCCCGA GCTGCCAGAT CCAAGCTTGG AAATGGGCAT TTTGCCCAGA
    GAGATCCGAA AACTGGTAGA GCGGAGAAGA CAAGTAAAAC AGCTAATGAA ACAGCAAGAT
    TTAAATCCAG ACCTTCATCT TCAGTATGAC ATTCGACAGA AGGCTTTGAA GCTCACGGCA
    AACAGTATGT ACGGCTGCCT GGGATTCTCC TACAGCAGAT TTTACGCCAA ACCCCTGGCT
    GCTTTGGTGA CATACAAAGG AAGGGAGATT TTGATGCATA CGAAGGAGAT GGTTCAGAAG
    ATGAATCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATCAACAC CAATAGCACC
    AATCTGGAAG AAGTGTTTAA ATTGGGAAAC AAGGTGAAAA GTGAAGTGAA TAAGTTGTAC
    AAACTGCTTG AAATAGACAT TGATGGTATT TTCAAGTCCC TGCTACTGCT GAAGAAAAAA
    AAGTATGCTG CTCTGATTGT TGAGCCAACA TCAGATGGGA ATTACGTCAC GAAACAGGAG
    GTGAAAGGAT TAGATATAGT TAGAAGAGAC TGGTGTGATC TTGCTAAAGA CACTGGAAAC
    TTTGTCATTG GCCAGATTCT TTCGGATCAA AACCGGGACA CTATAGTGGA AAACATTCAG
    AAGAGGTTAA TAGAAATTGG AGAAAATGTG CTAAACGGCA GTGTCCCAGT GAGCCAGTTT
    GAAATTAATA AGGCGTTGAC CAAGGATCCC CAGGATTACC CTGATAAAAA AAGCCTACCT
    CATGTACATG TTGCCCTCTG GATAAATTCT CAAGGAGGAA GAAAGGTGAA AGCTGGAGAT
    ACTGTGTCCT ATGTCATCTG TCAGGATGGT TCCAATCTCA CTGCGGGTCA GAGGGCCTAT
    GCACCCGAGC AGCTGCAGAA GCAGGACAAT TTAAGCATTG ATACCCAGTA CTACCTGGCC
    CAGCAGATCC ACCCAGTCGT GGCTCGAATA TGTGAGCCAA TAGATGGAAT TGATGCTATC
    CTCATTGCAT CCTGGTTAGG ACTTGACCCC ACCCAATTTA GGGTTCATCA TTATCATAAA
    GATGAAGAGA ATGATGCTTT ACTTGGTGGC CCAGCACACC TCACGGATGA AGAGAAATAC
    AAGGACTGTG AAAGGTTCAA ATGCCCATGC CCTACATGTG GAACCGAGAA TATTTATGAT
    AGTGTCTTAG ATGGTTCGGG AACTGATATG GAGCCCAGCT TGTATCGTTG CAGTAACATC
    AATTGTAAGG CTTCTCCTCT GACCTTTATG GTACAGCTGA GCAACAAATT GATCATGGAC
    ATTAGACGTT GCATTAAAAA ATACTATGAA GGCTGGTTGA TTTGTGAAGA GCCCACATGT
    CGAAATCGAA CTCGGCACCT TCCCCTTCAG TTCTCTCGAA ATGGACCCCT TTGCCAAGTC
    TGCATGAAAG CTACACTTCG ACTAGAGTAT TCTGACAAGT CCCTGTACAC CCAGCTGTGC
    TTTTACCGGT ACATTTTTGA TGCGGAGTGT GCACTGGAGA AACTTATTAC CGATCATGAG
    AAAGATAAAT TGAAGAAGCA GTTTTTTACT CCCAAAGTTC TTCAGGACTA TAGAAAACTC
    AAGAACACAG CAGAGCAGTT CTTGTCCCAA AGTGGCTATT CCGAAGTGAA CCTCAGCAAA
    CTCTTCGCCG ATTGTGCTGT ATGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89431
    Clone ID Related Accession (Same CDS sequence) XM_015461766.1
    Accession Version XM_015461766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGAGG GTCCACAGTA ATGAAGTGCA GAACAGCAAG TGCCAGTCAC
    CATGAAGATG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317252.1
    CDS178..3972
    Translation

    Target ORF information:

    RefSeq Version XM_015461766.1
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGAGG GTCCACAGTA ATGAAGTGCA GAACAGCAAG TGCCAGTCAC
    CATGAAGATG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40907
    Clone ID Related Accession (Same CDS sequence) NM_001206065.1
    Accession Version NM_001206065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000030.1. On Apr 20, 2011 this sequence version replaced XM_614762.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCT TGCATGGCGA GGATTCTGTG TCAGATTCCA GGAGTCTTGT AGCTTCCCGA 
    TCCCGGAGAG AGAAGAAATC AAAGAAAGGG CGACAGCAAG CTCTGGAAAG ACTGAAAAGG
    GCAAAAGCTG GTGAGAAGTA TAAATATGAA GTAGAGGACT TCACGAGTGT TTATGAAGAA
    GTTGATGAGG AACAGTACTC AAAGCTGGTT CAGGCCCGCC AGGATGACGA CTGGATCGTG
    GATGACGATG GTATTGGCTA TGTGGAGGAT GGCCGGGAAA TTTTTGATGA TGACCTTGAA
    GATGATGCTC TTGATTCCCA TGAGAAAGGA AAAGATAATA AAGCATGCAA CAAAGATAAG
    AGGACTGTTA AGAGGGCTGC TGTGACAAAA CCGAACAACA TCAAATCAAT GTTCATTGCC
    AGTGCTGGGA GGAAAACTGT AGATAAAGCT GTTGACTTGT CCAAGGATGA TCTGTTAGGT
    GACATTCTAC AGGACCTGAA CACTGAGACA CCACAAATAA CTCCACCACC TGTAATAATA
    CCAAAAAAGA AAAGATCCAG TGGAGCTTCA CTGAATCCTT TCTCTGTGCA CACCCCCAAG
    GCAGCTCCTT CAGGAAAAAC CACTCCCCCA GCCTCAAGGA GAGAGCCTTC ATTAACTCCT
    GCTCCTCTTA CATGTGCTGA ATTTGCTGGA GAGCCCGGGC CGCCACTGTG TACAGATGAC
    AAGGAGGAGT CGGGAGCAAT GGAATTTGAA GATGGCGACT TTGATGAGCC CATGGAAGCT
    GAGGAGGTGG ATGTGGAGCC CGTGGCTGCC AAGACTTTGT GCCAAGAGAG GGAGCCAGCA
    GAGGAGGCAA AACACAAGGC TGATTCTGGG AAAGGGACCA CATCCTCCTC AGCAAGTTTT
    CTCCCAGATG TCTCTTGTTG GGACATGGAT CAGGAAGATG ACAGCAGTTT CTCAGCCCTG
    GAAGTTCAAG TGGATTCCAG TCACCTCCCA CTGGTAAAAG GAGCTGATGA GGAACAAGTA
    TTTCAGTTTT ATTGGCTGGA TGCTTATGAA GATCCGTACA GCCAACCAGG TGTGGTATTT
    CTGTTTGGCA AGGTCTGGAT CGAATCTGCT GAAACCCACG TGAGCTGTTG TGTCATGGTG
    AAAAACATTG AGCGATCGCT CTGTTTCCTT CCCCGTGAAA TGAAAGTTGA TCTAAATACG
    GGGAAAGAAA GTGGAACTCC AGTTACCATG AAGGATGTTT ATGATGAATT TGATGAGAAA
    ATAGCAGCAA AATATAAGAT TATGAAATTC AAGTCCAAGG TAGTGGAAAA GAACTATGCT
    TTTGAGATAC CTGATGTTCC GGAGAAATCT GAGTACTTGG AAGTTAGATA CTCGGCTGAA
    ATGCCACAGC TTCCTCAGGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC
    ACATCCAGCC TGGAACTGTT CTTAATGAAT AGAAAGATCA AAGGACCTTG TTGGCTTGAA
    GTGAAAAATC CACAGCTCTT GAATCAGCCA ATCAGTTGGT GTAAAGTTGA GGCAATGGTC
    TTGAAACCGG ACCTGGTGAA CGTAATTAAG GATGTTGGTC CTCCTCCAGT TGTGGTCATG
    TCTTTGAGCA TGAAGACAAT GCAGAATGCA AAGACACATG AAAATGAGAT TATTGCTGTG
    TCAGCGTTGG TCCATCACAG CTTTGCATTA GATAAAGCAC CCCCACAGCC TCCCTTTCAG
    TCACACTTCT GCGTTGTGTC TAAACCAAAG GACTGCATTT TTCCATATGA TTTCAAAGAA
    AGCATTAAAG AAAAGAATGT GAAGGTTGAG GTTGCTGCCA CGGAAAGAAC ACTGCTAGGT
    TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT
    GGTTTTGAAC TGGAAGTGCT ACTGCAGAGA ATTAATGTGT GCAAAGTTCC TTTCTGGTCC
    AAGATAGGTC GGCTGAAGCG ATCCAACATG CCGAAGCTTG GGGGCAGGAG TGGATTTGGT
    GAAAAAAATG CTACCTGTGG CCGAATGATC TGTGATGTGG AAATTTCGGC GAAGGAACTG
    ATTCGGTGTA AAAGCTACCA TTTATCTGAA CTTGTTCAAC AGATTTTGAA AACTGAAAGG
    ATTGTAATCC CAATAGAAAC TATACGAAAC ATGTACAGTG ACTCTTCTCA TCTGTTGTAC
    CTGTTGGAGC ACACCTGGAA AGATGCCAGG TTCATTTTGC AGATCATGTG TGAGCTAAAT
    GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC TAGGACACTG
    ATGGGTGGAC GATCCGAGCG TAATGAGTTC TTACTACTTC ATGCATTTTA TGAAAACAAC
    TATATTGTGC CTGACAAGCA GACTTTCAGA AAGCCTCAGC AGAAACTGGG AGATGAAGAT
    GAAGACATTG ATGGAGACAC CAATAAATAC AAAAAAGGAC GTAAGAAAGC AGCTTATTCT
    GGAGGCTTGG TCTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC
    TTCAACAGTT TGTATCCTTC CATCATTCAG GAATTCAACA TTTGTTTTAC AACAGTGCAA
    AGAGTTGCTT CAGAGGCACA GAAAGTTGTA GAGGATGGAG AGCAAGAGCA GATCCCCGAG
    CTGCCAGATC CAAGCTTGGA AATGGGCATT TTGCCCAGAG AGATCCGAAA ACTGGTAGAG
    CGGAGAAGAC AAGTAAAACA GCTAATGAAA CAGCAAGATT TAAATCCAGA CCTTCATCTT
    CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACGGCAA ACAGTATGTA CGGCTGCCTG
    GGATTCTCCT ACAGCAGATT TTACGCCAAA CCCCTGGCTG CTTTGGTGAC ATACAAAGGA
    AGGGAGATTT TGATGCATAC GAAGGAGATG GTTCAGAAGA TGAATCTTGA AGTTATTTAT
    GGAGATACAG ATTCAATTAT GATCAACACC AATAGCACCA ATCTGGAAGA AGTGTTTAAA
    TTGGGAAACA AGGTGAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT
    GATGGTATTT TCAAGTCCCT GCTACTGCTG AAGAAAAAAA AGTATGCTGC TCTGATTGTT
    GAGCCAACAT CAGATGGGAA TTACGTCACG AAACAGGAGG TGAAAGGATT AGATATAGTT
    AGAAGAGACT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTCATTGG CCAGATTCTT
    TCGGATCAAA ACCGGGACAC TATAGTGGAA AACATTCAGA AGAGGTTAAT AGAAATTGGA
    GAAAATGTGC TAAACGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAATAA GGCGTTGACC
    AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG
    ATAAATTCTC AAGGAGGAAG AAAGGTGAAA GCTGGAGATA CTGTGTCCTA TGTCATCTGT
    CAGGATGGTT CCAATCTCAC TGCGGGTCAG AGGGCCTATG CACCCGAGCA GCTGCAGAAG
    CAGGACAATT TAAGCATTGA TACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG
    GCTCGAATAT GTGAGCCAAT AGATGGAATT GATGCTATCC TCATTGCATC CTGGTTAGGA
    CTTGACCCCA CCCAATTTAG GGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTTTA
    CTTGGTGGCC CAGCACACCT CACGGATGAA GAGAAATACA AGGACTGTGA AAGGTTCAAA
    TGCCCATGCC CTACATGTGG AACCGAGAAT ATTTATGATA GTGTCTTAGA TGGTTCGGGA
    ACTGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCA ATTGTAAGGC TTCTCCTCTG
    ACCTTTATGG TACAGCTGAG CAACAAATTG ATCATGGACA TTAGACGTTG CATTAAAAAA
    TACTATGAAG GCTGGTTGAT TTGTGAAGAG CCCACATGTC GAAATCGAAC TCGGCACCTT
    CCCCTTCAGT TCTCTCGAAA TGGACCCCTT TGCCAAGTCT GCATGAAAGC TACACTTCGA
    CTAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT
    GCGGAGTGTG CACTGGAGAA ACTTATTACC GATCATGAGA AAGATAAATT GAAGAAGCAG
    TTTTTTACTC CCAAAGTTCT TCAGGACTAT AGAAAACTCA AGAACACAGC AGAGCAGTTC
    TTGTCCCAAA GTGGCTATTC CGAAGTGAAC CTCAGCAAAC TCTTCGCCGA TTGTGCTGTA
    TGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192994.1
    CDS1..4389
    Translation

    Target ORF information:

    RefSeq Version NM_001206065.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCT TGCATGGCGA GGATTCTGTG TCAGATTCCA GGAGTCTTGT AGCTTCCCGA 
    TCCCGGAGAG AGAAGAAATC AAAGAAAGGG CGACAGCAAG CTCTGGAAAG ACTGAAAAGG
    GCAAAAGCTG GTGAGAAGTA TAAATATGAA GTAGAGGACT TCACGAGTGT TTATGAAGAA
    GTTGATGAGG AACAGTACTC AAAGCTGGTT CAGGCCCGCC AGGATGACGA CTGGATCGTG
    GATGACGATG GTATTGGCTA TGTGGAGGAT GGCCGGGAAA TTTTTGATGA TGACCTTGAA
    GATGATGCTC TTGATTCCCA TGAGAAAGGA AAAGATAATA AAGCATGCAA CAAAGATAAG
    AGGACTGTTA AGAGGGCTGC TGTGACAAAA CCGAACAACA TCAAATCAAT GTTCATTGCC
    AGTGCTGGGA GGAAAACTGT AGATAAAGCT GTTGACTTGT CCAAGGATGA TCTGTTAGGT
    GACATTCTAC AGGACCTGAA CACTGAGACA CCACAAATAA CTCCACCACC TGTAATAATA
    CCAAAAAAGA AAAGATCCAG TGGAGCTTCA CTGAATCCTT TCTCTGTGCA CACCCCCAAG
    GCAGCTCCTT CAGGAAAAAC CACTCCCCCA GCCTCAAGGA GAGAGCCTTC ATTAACTCCT
    GCTCCTCTTA CATGTGCTGA ATTTGCTGGA GAGCCCGGGC CGCCACTGTG TACAGATGAC
    AAGGAGGAGT CGGGAGCAAT GGAATTTGAA GATGGCGACT TTGATGAGCC CATGGAAGCT
    GAGGAGGTGG ATGTGGAGCC CGTGGCTGCC AAGACTTTGT GCCAAGAGAG GGAGCCAGCA
    GAGGAGGCAA AACACAAGGC TGATTCTGGG AAAGGGACCA CATCCTCCTC AGCAAGTTTT
    CTCCCAGATG TCTCTTGTTG GGACATGGAT CAGGAAGATG ACAGCAGTTT CTCAGCCCTG
    GAAGTTCAAG TGGATTCCAG TCACCTCCCA CTGGTAAAAG GAGCTGATGA GGAACAAGTA
    TTTCAGTTTT ATTGGCTGGA TGCTTATGAA GATCCGTACA GCCAACCAGG TGTGGTATTT
    CTGTTTGGCA AGGTCTGGAT CGAATCTGCT GAAACCCACG TGAGCTGTTG TGTCATGGTG
    AAAAACATTG AGCGATCGCT CTGTTTCCTT CCCCGTGAAA TGAAAGTTGA TCTAAATACG
    GGGAAAGAAA GTGGAACTCC AGTTACCATG AAGGATGTTT ATGATGAATT TGATGAGAAA
    ATAGCAGCAA AATATAAGAT TATGAAATTC AAGTCCAAGG TAGTGGAAAA GAACTATGCT
    TTTGAGATAC CTGATGTTCC GGAGAAATCT GAGTACTTGG AAGTTAGATA CTCGGCTGAA
    ATGCCACAGC TTCCTCAGGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC
    ACATCCAGCC TGGAACTGTT CTTAATGAAT AGAAAGATCA AAGGACCTTG TTGGCTTGAA
    GTGAAAAATC CACAGCTCTT GAATCAGCCA ATCAGTTGGT GTAAAGTTGA GGCAATGGTC
    TTGAAACCGG ACCTGGTGAA CGTAATTAAG GATGTTGGTC CTCCTCCAGT TGTGGTCATG
    TCTTTGAGCA TGAAGACAAT GCAGAATGCA AAGACACATG AAAATGAGAT TATTGCTGTG
    TCAGCGTTGG TCCATCACAG CTTTGCATTA GATAAAGCAC CCCCACAGCC TCCCTTTCAG
    TCACACTTCT GCGTTGTGTC TAAACCAAAG GACTGCATTT TTCCATATGA TTTCAAAGAA
    AGCATTAAAG AAAAGAATGT GAAGGTTGAG GTTGCTGCCA CGGAAAGAAC ACTGCTAGGT
    TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT
    GGTTTTGAAC TGGAAGTGCT ACTGCAGAGA ATTAATGTGT GCAAAGTTCC TTTCTGGTCC
    AAGATAGGTC GGCTGAAGCG ATCCAACATG CCGAAGCTTG GGGGCAGGAG TGGATTTGGT
    GAAAAAAATG CTACCTGTGG CCGAATGATC TGTGATGTGG AAATTTCGGC GAAGGAACTG
    ATTCGGTGTA AAAGCTACCA TTTATCTGAA CTTGTTCAAC AGATTTTGAA AACTGAAAGG
    ATTGTAATCC CAATAGAAAC TATACGAAAC ATGTACAGTG ACTCTTCTCA TCTGTTGTAC
    CTGTTGGAGC ACACCTGGAA AGATGCCAGG TTCATTTTGC AGATCATGTG TGAGCTAAAT
    GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC TAGGACACTG
    ATGGGTGGAC GATCCGAGCG TAATGAGTTC TTACTACTTC ATGCATTTTA TGAAAACAAC
    TATATTGTGC CTGACAAGCA GACTTTCAGA AAGCCTCAGC AGAAACTGGG AGATGAAGAT
    GAAGACATTG ATGGAGACAC CAATAAATAC AAAAAAGGAC GTAAGAAAGC AGCTTATTCT
    GGAGGCTTGG TCTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC
    TTCAACAGTT TGTATCCTTC CATCATTCAG GAATTCAACA TTTGTTTTAC AACAGTGCAA
    AGAGTTGCTT CAGAGGCACA GAAAGTTGTA GAGGATGGAG AGCAAGAGCA GATCCCCGAG
    CTGCCAGATC CAAGCTTGGA AATGGGCATT TTGCCCAGAG AGATCCGAAA ACTGGTAGAG
    CGGAGAAGAC AAGTAAAACA GCTAATGAAA CAGCAAGATT TAAATCCAGA CCTTCATCTT
    CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACGGCAA ACAGTATGTA CGGCTGCCTG
    GGATTCTCCT ACAGCAGATT TTACGCCAAA CCCCTGGCTG CTTTGGTGAC ATACAAAGGA
    AGGGAGATTT TGATGCATAC GAAGGAGATG GTTCAGAAGA TGAATCTTGA AGTTATTTAT
    GGAGATACAG ATTCAATTAT GATCAACACC AATAGCACCA ATCTGGAAGA AGTGTTTAAA
    TTGGGAAACA AGGTGAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT
    GATGGTATTT TCAAGTCCCT GCTACTGCTG AAGAAAAAAA AGTATGCTGC TCTGATTGTT
    GAGCCAACAT CAGATGGGAA TTACGTCACG AAACAGGAGG TGAAAGGATT AGATATAGTT
    AGAAGAGACT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTCATTGG CCAGATTCTT
    TCGGATCAAA ACCGGGACAC TATAGTGGAA AACATTCAGA AGAGGTTAAT AGAAATTGGA
    GAAAATGTGC TAAACGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAATAA GGCGTTGACC
    AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG
    ATAAATTCTC AAGGAGGAAG AAAGGTGAAA GCTGGAGATA CTGTGTCCTA TGTCATCTGT
    CAGGATGGTT CCAATCTCAC TGCGGGTCAG AGGGCCTATG CACCCGAGCA GCTGCAGAAG
    CAGGACAATT TAAGCATTGA TACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG
    GCTCGAATAT GTGAGCCAAT AGATGGAATT GATGCTATCC TCATTGCATC CTGGTTAGGA
    CTTGACCCCA CCCAATTTAG GGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTTTA
    CTTGGTGGCC CAGCACACCT CACGGATGAA GAGAAATACA AGGACTGTGA AAGGTTCAAA
    TGCCCATGCC CTACATGTGG AACCGAGAAT ATTTATGATA GTGTCTTAGA TGGTTCGGGA
    ACTGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCA ATTGTAAGGC TTCTCCTCTG
    ACCTTTATGG TACAGCTGAG CAACAAATTG ATCATGGACA TTAGACGTTG CATTAAAAAA
    TACTATGAAG GCTGGTTGAT TTGTGAAGAG CCCACATGTC GAAATCGAAC TCGGCACCTT
    CCCCTTCAGT TCTCTCGAAA TGGACCCCTT TGCCAAGTCT GCATGAAAGC TACACTTCGA
    CTAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT
    GCGGAGTGTG CACTGGAGAA ACTTATTACC GATCATGAGA AAGATAAATT GAAGAAGCAG
    TTTTTTACTC CCAAAGTTCT TCAGGACTAT AGAAAACTCA AGAACACAGC AGAGCAGTTC
    TTGTCCCAAA GTGGCTATTC CGAAGTGAAC CTCAGCAAAC TCTTCGCCGA TTGTGCTGTA
    TGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35737
    Clone ID Related Accession (Same CDS sequence) XM_005228356.3 , XM_005228356.4
    Accession Version XM_005228356.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG
    AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC
    TTCGCCGATT GTGCTGTATG GTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228413.1
    CDS175..4581
    Translation

    Target ORF information:

    RefSeq Version XM_005228356.3
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228356.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG
    AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC
    TTCGCCGATT GTGCTGTATG GTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35737
    Clone ID Related Accession (Same CDS sequence) XM_005228356.3 , XM_005228356.4
    Accession Version XM_005228356.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228356.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG
    AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC
    TTCGCCGATT GTGCTGTATG GTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228413.1
    CDS196..4602
    Translation

    Target ORF information:

    RefSeq Version XM_005228356.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228356.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG
    AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC
    TTCGCCGATT GTGCTGTATG GTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35739
    Clone ID Related Accession (Same CDS sequence) XM_005228359.4 , XM_005228359.3
    Accession Version XM_005228359.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA
    AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA
    ACTGCATCTG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228416.1
    CDS196..4470
    Translation

    Target ORF information:

    RefSeq Version XM_005228359.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228359.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA
    AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA
    ACTGCATCTG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35739
    Clone ID Related Accession (Same CDS sequence) XM_005228359.4 , XM_005228359.3
    Accession Version XM_005228359.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA
    AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA
    ACTGCATCTG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228416.1
    CDS176..4450
    Translation

    Target ORF information:

    RefSeq Version XM_005228359.3
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA
    AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA
    ACTGCATCTG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35740
    Clone ID Related Accession (Same CDS sequence) XM_005228360.3 , XM_005228360.4
    Accession Version XM_005228360.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT
    GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228417.1
    CDS176..4414
    Translation

    Target ORF information:

    RefSeq Version XM_005228360.3
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228360.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT
    GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35740
    Clone ID Related Accession (Same CDS sequence) XM_005228360.3 , XM_005228360.4
    Accession Version XM_005228360.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase alpha catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228360.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT
    GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228417.1
    CDS196..4434
    Translation

    Target ORF information:

    RefSeq Version XM_005228360.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228360.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
    AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT
    CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC
    ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG
    GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT
    TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA
    GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC
    AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC
    AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT
    CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC
    TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA
    GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG
    CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT
    GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC
    CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA
    TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC
    AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA
    GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC
    CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG
    AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG
    AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT
    GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA
    GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT
    AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT
    CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA
    AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC
    CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT
    CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC
    AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG
    GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA
    ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG
    ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC
    TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT
    GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG
    AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT
    AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT
    TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG
    CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA
    AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC
    AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT
    CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG
    TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG
    AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT
    GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA
    CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC
    ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG
    GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT
    GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT
    TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA
    AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC
    ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT
    GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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