POLA1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015461765.1	XP_015317251.1	DNA polymerase alpha catalytic subunit isoform X2
XM_024988125.1	XP_024843893.1	DNA polymerase alpha catalytic subunit isoform X4
XM_005228356.3	XP_005228413.1	DNA polymerase alpha catalytic subunit isoform X1
XM_005228360.3	XP_005228417.1	DNA polymerase alpha catalytic subunit isoform X4
XM_005228356.4	XP_005228413.1	DNA polymerase alpha catalytic subunit isoform X1
XM_005228360.4	XP_005228417.1	DNA polymerase alpha catalytic subunit isoform X3
NM_001206065.1	NP_001192994.1	DNA polymerase alpha catalytic subunit
XM_005228359.4	XP_005228416.1	DNA polymerase alpha catalytic subunit isoform X2
XM_005228359.3	XP_005228416.1	DNA polymerase alpha catalytic subunit isoform X3
XM_015461766.1	XP_015317252.1	DNA polymerase alpha catalytic subunit isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238447	XM_024988125.1 Latest version!	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89430	XM_015461765.1 Latest version!	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa89431	XM_015461766.1 Latest version!	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa40907	NM_001206065.1 Latest version!	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa35737	XM_005228356.4 Latest version!	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa35739	XM_005228359.4 Latest version!	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa35740	XM_005228360.4 Latest version!	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa35737	XM_005228356.3	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa35740	XM_005228360.3	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa35739	XM_005228359.3	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

POLA1 ( XM_024988125.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843893.1 Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa238447
Clone ID Related Accession (Same CDS sequence)	XM_024988125.1
Accession Version	XM_024988125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGATAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843893.1
CDS	196..4368
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEIN

Target ORF information:

RefSeq Version	XM_024988125.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988125.1

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGATAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_015461765.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317251.1 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa89430
Clone ID Related Accession (Same CDS sequence)	XM_015461765.1
Accession Version	XM_015461765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGCAGGA GTGGATTTGG TGAAAAAAAT GCTACCTGTG GCCGAATGAT CTGTGATGTG 
GAAATTTCGG CGAAGGAACT GATTCGGTGT AAAAGCTACC ATTTATCTGA ACTTGTTCAA 
CAGATTTTGA AAACTGAAAG GATTGTAATC CCAATAGAAA CTATACGAAA CATGTACAGT 
GACTCTTCTC ATCTGTTGTA CCTGTTGGAG CACACCTGGA AAGATGCCAG GTTCATTTTG 
CAGATCATGT GTGAGCTAAA TGTTCTTCCA TTAGCATTGC AGATCACTAA CATCGCTGGG 
AACATTATGT CTAGGACACT GATGGGTGGA CGATCCGAGC GTAATGAGTT CTTACTACTT 
CATGCATTTT ATGAAAACAA CTATATTGTG CCTGACAAGC AGACTTTCAG AAAGCCTCAG 
CAGAAACTGG GAGATGAAGA TGAAGACATT GATGGAGACA CCAATAAATA CAAAAAAGGA 
CGTAAGAAAG CAGCTTATTC TGGAGGCTTG GTCTTGGACC CCAAAGTTGG TTTTTATGAT 
AAGTTCATTT TGCTTCTGGA CTTCAACAGT TTGTATCCTT CCATCATTCA GGAATTCAAC 
ATTTGTTTTA CAACAGTGCA AAGAGTTGCT TCAGAGGCAC AGAAAGTTGT AGAGGATGGA 
GAGCAAGAGC AGATCCCCGA GCTGCCAGAT CCAAGCTTGG AAATGGGCAT TTTGCCCAGA 
GAGATCCGAA AACTGGTAGA GCGGAGAAGA CAAGTAAAAC AGCTAATGAA ACAGCAAGAT 
TTAAATCCAG ACCTTCATCT TCAGTATGAC ATTCGACAGA AGGCTTTGAA GCTCACGGCA 
AACAGTATGT ACGGCTGCCT GGGATTCTCC TACAGCAGAT TTTACGCCAA ACCCCTGGCT 
GCTTTGGTGA CATACAAAGG AAGGGAGATT TTGATGCATA CGAAGGAGAT GGTTCAGAAG 
ATGAATCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATCAACAC CAATAGCACC 
AATCTGGAAG AAGTGTTTAA ATTGGGAAAC AAGGTGAAAA GTGAAGTGAA TAAGTTGTAC 
AAACTGCTTG AAATAGACAT TGATGGTATT TTCAAGTCCC TGCTACTGCT GAAGAAAAAA 
AAGTATGCTG CTCTGATTGT TGAGCCAACA TCAGATGGGA ATTACGTCAC GAAACAGGAG 
GTGAAAGGAT TAGATATAGT TAGAAGAGAC TGGTGTGATC TTGCTAAAGA CACTGGAAAC 
TTTGTCATTG GCCAGATTCT TTCGGATCAA AACCGGGACA CTATAGTGGA AAACATTCAG 
AAGAGGTTAA TAGAAATTGG AGAAAATGTG CTAAACGGCA GTGTCCCAGT GAGCCAGTTT 
GAAATTAATA AGGCGTTGAC CAAGGATCCC CAGGATTACC CTGATAAAAA AAGCCTACCT 
CATGTACATG TTGCCCTCTG GATAAATTCT CAAGGAGGAA GAAAGGTGAA AGCTGGAGAT 
ACTGTGTCCT ATGTCATCTG TCAGGATGGT TCCAATCTCA CTGCGGGTCA GAGGGCCTAT 
GCACCCGAGC AGCTGCAGAA GCAGGACAAT TTAAGCATTG ATACCCAGTA CTACCTGGCC 
CAGCAGATCC ACCCAGTCGT GGCTCGAATA TGTGAGCCAA TAGATGGAAT TGATGCTATC 
CTCATTGCAT CCTGGTTAGG ACTTGACCCC ACCCAATTTA GGGTTCATCA TTATCATAAA 
GATGAAGAGA ATGATGCTTT ACTTGGTGGC CCAGCACACC TCACGGATGA AGAGAAATAC 
AAGGACTGTG AAAGGTTCAA ATGCCCATGC CCTACATGTG GAACCGAGAA TATTTATGAT 
AGTGTCTTAG ATGGTTCGGG AACTGATATG GAGCCCAGCT TGTATCGTTG CAGTAACATC 
AATTGTAAGG CTTCTCCTCT GACCTTTATG GTACAGCTGA GCAACAAATT GATCATGGAC 
ATTAGACGTT GCATTAAAAA ATACTATGAA GGCTGGTTGA TTTGTGAAGA GCCCACATGT 
CGAAATCGAA CTCGGCACCT TCCCCTTCAG TTCTCTCGAA ATGGACCCCT TTGCCAAGTC 
TGCATGAAAG CTACACTTCG ACTAGAGTAT TCTGACAAGT CCCTGTACAC CCAGCTGTGC 
TTTTACCGGT ACATTTTTGA TGCGGAGTGT GCACTGGAGA AACTTATTAC CGATCATGAG 
AAAGATAAAT TGAAGAAGCA GTTTTTTACT CCCAAAGTTC TTCAGGACTA TAGAAAACTC 
AAGAACACAG CAGAGCAGTT CTTGTCCCAA AGTGGCTATT CCGAAGTGAA CCTCAGCAAA 
CTCTTCGCCG ATTGTGCTGT ATGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317251.1
CDS	175..4464
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEYSDKSLYTQLCFYRYIFDAECALEKLITDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSQSGYSEVNLSKLFADCAVWS

Target ORF information:

RefSeq Version	XM_015461765.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461765.1

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGCAGGA GTGGATTTGG TGAAAAAAAT GCTACCTGTG GCCGAATGAT CTGTGATGTG 
GAAATTTCGG CGAAGGAACT GATTCGGTGT AAAAGCTACC ATTTATCTGA ACTTGTTCAA 
CAGATTTTGA AAACTGAAAG GATTGTAATC CCAATAGAAA CTATACGAAA CATGTACAGT 
GACTCTTCTC ATCTGTTGTA CCTGTTGGAG CACACCTGGA AAGATGCCAG GTTCATTTTG 
CAGATCATGT GTGAGCTAAA TGTTCTTCCA TTAGCATTGC AGATCACTAA CATCGCTGGG 
AACATTATGT CTAGGACACT GATGGGTGGA CGATCCGAGC GTAATGAGTT CTTACTACTT 
CATGCATTTT ATGAAAACAA CTATATTGTG CCTGACAAGC AGACTTTCAG AAAGCCTCAG 
CAGAAACTGG GAGATGAAGA TGAAGACATT GATGGAGACA CCAATAAATA CAAAAAAGGA 
CGTAAGAAAG CAGCTTATTC TGGAGGCTTG GTCTTGGACC CCAAAGTTGG TTTTTATGAT 
AAGTTCATTT TGCTTCTGGA CTTCAACAGT TTGTATCCTT CCATCATTCA GGAATTCAAC 
ATTTGTTTTA CAACAGTGCA AAGAGTTGCT TCAGAGGCAC AGAAAGTTGT AGAGGATGGA 
GAGCAAGAGC AGATCCCCGA GCTGCCAGAT CCAAGCTTGG AAATGGGCAT TTTGCCCAGA 
GAGATCCGAA AACTGGTAGA GCGGAGAAGA CAAGTAAAAC AGCTAATGAA ACAGCAAGAT 
TTAAATCCAG ACCTTCATCT TCAGTATGAC ATTCGACAGA AGGCTTTGAA GCTCACGGCA 
AACAGTATGT ACGGCTGCCT GGGATTCTCC TACAGCAGAT TTTACGCCAA ACCCCTGGCT 
GCTTTGGTGA CATACAAAGG AAGGGAGATT TTGATGCATA CGAAGGAGAT GGTTCAGAAG 
ATGAATCTTG AAGTTATTTA TGGAGATACA GATTCAATTA TGATCAACAC CAATAGCACC 
AATCTGGAAG AAGTGTTTAA ATTGGGAAAC AAGGTGAAAA GTGAAGTGAA TAAGTTGTAC 
AAACTGCTTG AAATAGACAT TGATGGTATT TTCAAGTCCC TGCTACTGCT GAAGAAAAAA 
AAGTATGCTG CTCTGATTGT TGAGCCAACA TCAGATGGGA ATTACGTCAC GAAACAGGAG 
GTGAAAGGAT TAGATATAGT TAGAAGAGAC TGGTGTGATC TTGCTAAAGA CACTGGAAAC 
TTTGTCATTG GCCAGATTCT TTCGGATCAA AACCGGGACA CTATAGTGGA AAACATTCAG 
AAGAGGTTAA TAGAAATTGG AGAAAATGTG CTAAACGGCA GTGTCCCAGT GAGCCAGTTT 
GAAATTAATA AGGCGTTGAC CAAGGATCCC CAGGATTACC CTGATAAAAA AAGCCTACCT 
CATGTACATG TTGCCCTCTG GATAAATTCT CAAGGAGGAA GAAAGGTGAA AGCTGGAGAT 
ACTGTGTCCT ATGTCATCTG TCAGGATGGT TCCAATCTCA CTGCGGGTCA GAGGGCCTAT 
GCACCCGAGC AGCTGCAGAA GCAGGACAAT TTAAGCATTG ATACCCAGTA CTACCTGGCC 
CAGCAGATCC ACCCAGTCGT GGCTCGAATA TGTGAGCCAA TAGATGGAAT TGATGCTATC 
CTCATTGCAT CCTGGTTAGG ACTTGACCCC ACCCAATTTA GGGTTCATCA TTATCATAAA 
GATGAAGAGA ATGATGCTTT ACTTGGTGGC CCAGCACACC TCACGGATGA AGAGAAATAC 
AAGGACTGTG AAAGGTTCAA ATGCCCATGC CCTACATGTG GAACCGAGAA TATTTATGAT 
AGTGTCTTAG ATGGTTCGGG AACTGATATG GAGCCCAGCT TGTATCGTTG CAGTAACATC 
AATTGTAAGG CTTCTCCTCT GACCTTTATG GTACAGCTGA GCAACAAATT GATCATGGAC 
ATTAGACGTT GCATTAAAAA ATACTATGAA GGCTGGTTGA TTTGTGAAGA GCCCACATGT 
CGAAATCGAA CTCGGCACCT TCCCCTTCAG TTCTCTCGAA ATGGACCCCT TTGCCAAGTC 
TGCATGAAAG CTACACTTCG ACTAGAGTAT TCTGACAAGT CCCTGTACAC CCAGCTGTGC 
TTTTACCGGT ACATTTTTGA TGCGGAGTGT GCACTGGAGA AACTTATTAC CGATCATGAG 
AAAGATAAAT TGAAGAAGCA GTTTTTTACT CCCAAAGTTC TTCAGGACTA TAGAAAACTC 
AAGAACACAG CAGAGCAGTT CTTGTCCCAA AGTGGCTATT CCGAAGTGAA CCTCAGCAAA 
CTCTTCGCCG ATTGTGCTGT ATGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_015461766.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317252.1 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA.

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CloneID	OBa89431
Clone ID Related Accession (Same CDS sequence)	XM_015461766.1
Accession Version	XM_015461766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGAGG GTCCACAGTA ATGAAGTGCA GAACAGCAAG TGCCAGTCAC 
CATGAAGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317252.1
CDS	178..3972
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGGSTVMKCRTASASHHEDV

Target ORF information:

RefSeq Version	XM_015461766.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461766.1

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGAGG GTCCACAGTA ATGAAGTGCA GAACAGCAAG TGCCAGTCAC 
CATGAAGATG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( NM_001206065.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192994.1 Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.

Price & Availability↑

CloneID	OBa40907
Clone ID Related Accession (Same CDS sequence)	NM_001206065.1
Accession Version	NM_001206065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000030.1. On Apr 20, 2011 this sequence version replaced XM_614762.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTCTGTG TCAGATTCCA GGAGTCTTGT AGCTTCCCGA 
TCCCGGAGAG AGAAGAAATC AAAGAAAGGG CGACAGCAAG CTCTGGAAAG ACTGAAAAGG 
GCAAAAGCTG GTGAGAAGTA TAAATATGAA GTAGAGGACT TCACGAGTGT TTATGAAGAA 
GTTGATGAGG AACAGTACTC AAAGCTGGTT CAGGCCCGCC AGGATGACGA CTGGATCGTG 
GATGACGATG GTATTGGCTA TGTGGAGGAT GGCCGGGAAA TTTTTGATGA TGACCTTGAA 
GATGATGCTC TTGATTCCCA TGAGAAAGGA AAAGATAATA AAGCATGCAA CAAAGATAAG 
AGGACTGTTA AGAGGGCTGC TGTGACAAAA CCGAACAACA TCAAATCAAT GTTCATTGCC 
AGTGCTGGGA GGAAAACTGT AGATAAAGCT GTTGACTTGT CCAAGGATGA TCTGTTAGGT 
GACATTCTAC AGGACCTGAA CACTGAGACA CCACAAATAA CTCCACCACC TGTAATAATA 
CCAAAAAAGA AAAGATCCAG TGGAGCTTCA CTGAATCCTT TCTCTGTGCA CACCCCCAAG 
GCAGCTCCTT CAGGAAAAAC CACTCCCCCA GCCTCAAGGA GAGAGCCTTC ATTAACTCCT 
GCTCCTCTTA CATGTGCTGA ATTTGCTGGA GAGCCCGGGC CGCCACTGTG TACAGATGAC 
AAGGAGGAGT CGGGAGCAAT GGAATTTGAA GATGGCGACT TTGATGAGCC CATGGAAGCT 
GAGGAGGTGG ATGTGGAGCC CGTGGCTGCC AAGACTTTGT GCCAAGAGAG GGAGCCAGCA 
GAGGAGGCAA AACACAAGGC TGATTCTGGG AAAGGGACCA CATCCTCCTC AGCAAGTTTT 
CTCCCAGATG TCTCTTGTTG GGACATGGAT CAGGAAGATG ACAGCAGTTT CTCAGCCCTG 
GAAGTTCAAG TGGATTCCAG TCACCTCCCA CTGGTAAAAG GAGCTGATGA GGAACAAGTA 
TTTCAGTTTT ATTGGCTGGA TGCTTATGAA GATCCGTACA GCCAACCAGG TGTGGTATTT 
CTGTTTGGCA AGGTCTGGAT CGAATCTGCT GAAACCCACG TGAGCTGTTG TGTCATGGTG 
AAAAACATTG AGCGATCGCT CTGTTTCCTT CCCCGTGAAA TGAAAGTTGA TCTAAATACG 
GGGAAAGAAA GTGGAACTCC AGTTACCATG AAGGATGTTT ATGATGAATT TGATGAGAAA 
ATAGCAGCAA AATATAAGAT TATGAAATTC AAGTCCAAGG TAGTGGAAAA GAACTATGCT 
TTTGAGATAC CTGATGTTCC GGAGAAATCT GAGTACTTGG AAGTTAGATA CTCGGCTGAA 
ATGCCACAGC TTCCTCAGGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC 
ACATCCAGCC TGGAACTGTT CTTAATGAAT AGAAAGATCA AAGGACCTTG TTGGCTTGAA 
GTGAAAAATC CACAGCTCTT GAATCAGCCA ATCAGTTGGT GTAAAGTTGA GGCAATGGTC 
TTGAAACCGG ACCTGGTGAA CGTAATTAAG GATGTTGGTC CTCCTCCAGT TGTGGTCATG 
TCTTTGAGCA TGAAGACAAT GCAGAATGCA AAGACACATG AAAATGAGAT TATTGCTGTG 
TCAGCGTTGG TCCATCACAG CTTTGCATTA GATAAAGCAC CCCCACAGCC TCCCTTTCAG 
TCACACTTCT GCGTTGTGTC TAAACCAAAG GACTGCATTT TTCCATATGA TTTCAAAGAA 
AGCATTAAAG AAAAGAATGT GAAGGTTGAG GTTGCTGCCA CGGAAAGAAC ACTGCTAGGT 
TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT 
GGTTTTGAAC TGGAAGTGCT ACTGCAGAGA ATTAATGTGT GCAAAGTTCC TTTCTGGTCC 
AAGATAGGTC GGCTGAAGCG ATCCAACATG CCGAAGCTTG GGGGCAGGAG TGGATTTGGT 
GAAAAAAATG CTACCTGTGG CCGAATGATC TGTGATGTGG AAATTTCGGC GAAGGAACTG 
ATTCGGTGTA AAAGCTACCA TTTATCTGAA CTTGTTCAAC AGATTTTGAA AACTGAAAGG 
ATTGTAATCC CAATAGAAAC TATACGAAAC ATGTACAGTG ACTCTTCTCA TCTGTTGTAC 
CTGTTGGAGC ACACCTGGAA AGATGCCAGG TTCATTTTGC AGATCATGTG TGAGCTAAAT 
GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC TAGGACACTG 
ATGGGTGGAC GATCCGAGCG TAATGAGTTC TTACTACTTC ATGCATTTTA TGAAAACAAC 
TATATTGTGC CTGACAAGCA GACTTTCAGA AAGCCTCAGC AGAAACTGGG AGATGAAGAT 
GAAGACATTG ATGGAGACAC CAATAAATAC AAAAAAGGAC GTAAGAAAGC AGCTTATTCT 
GGAGGCTTGG TCTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC 
TTCAACAGTT TGTATCCTTC CATCATTCAG GAATTCAACA TTTGTTTTAC AACAGTGCAA 
AGAGTTGCTT CAGAGGCACA GAAAGTTGTA GAGGATGGAG AGCAAGAGCA GATCCCCGAG 
CTGCCAGATC CAAGCTTGGA AATGGGCATT TTGCCCAGAG AGATCCGAAA ACTGGTAGAG 
CGGAGAAGAC AAGTAAAACA GCTAATGAAA CAGCAAGATT TAAATCCAGA CCTTCATCTT 
CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACGGCAA ACAGTATGTA CGGCTGCCTG 
GGATTCTCCT ACAGCAGATT TTACGCCAAA CCCCTGGCTG CTTTGGTGAC ATACAAAGGA 
AGGGAGATTT TGATGCATAC GAAGGAGATG GTTCAGAAGA TGAATCTTGA AGTTATTTAT 
GGAGATACAG ATTCAATTAT GATCAACACC AATAGCACCA ATCTGGAAGA AGTGTTTAAA 
TTGGGAAACA AGGTGAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT 
GATGGTATTT TCAAGTCCCT GCTACTGCTG AAGAAAAAAA AGTATGCTGC TCTGATTGTT 
GAGCCAACAT CAGATGGGAA TTACGTCACG AAACAGGAGG TGAAAGGATT AGATATAGTT 
AGAAGAGACT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTCATTGG CCAGATTCTT 
TCGGATCAAA ACCGGGACAC TATAGTGGAA AACATTCAGA AGAGGTTAAT AGAAATTGGA 
GAAAATGTGC TAAACGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAATAA GGCGTTGACC 
AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG 
ATAAATTCTC AAGGAGGAAG AAAGGTGAAA GCTGGAGATA CTGTGTCCTA TGTCATCTGT 
CAGGATGGTT CCAATCTCAC TGCGGGTCAG AGGGCCTATG CACCCGAGCA GCTGCAGAAG 
CAGGACAATT TAAGCATTGA TACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG 
GCTCGAATAT GTGAGCCAAT AGATGGAATT GATGCTATCC TCATTGCATC CTGGTTAGGA 
CTTGACCCCA CCCAATTTAG GGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTTTA 
CTTGGTGGCC CAGCACACCT CACGGATGAA GAGAAATACA AGGACTGTGA AAGGTTCAAA 
TGCCCATGCC CTACATGTGG AACCGAGAAT ATTTATGATA GTGTCTTAGA TGGTTCGGGA 
ACTGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCA ATTGTAAGGC TTCTCCTCTG 
ACCTTTATGG TACAGCTGAG CAACAAATTG ATCATGGACA TTAGACGTTG CATTAAAAAA 
TACTATGAAG GCTGGTTGAT TTGTGAAGAG CCCACATGTC GAAATCGAAC TCGGCACCTT 
CCCCTTCAGT TCTCTCGAAA TGGACCCCTT TGCCAAGTCT GCATGAAAGC TACACTTCGA 
CTAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT 
GCGGAGTGTG CACTGGAGAA ACTTATTACC GATCATGAGA AAGATAAATT GAAGAAGCAG 
TTTTTTACTC CCAAAGTTCT TCAGGACTAT AGAAAACTCA AGAACACAGC AGAGCAGTTC 
TTGTCCCAAA GTGGCTATTC CGAAGTGAAC CTCAGCAAAC TCTTCGCCGA TTGTGCTGTA 
TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192994.1
CDS	1..4389
Translation	MAPLHGEDSVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEYSDKSLYTQLCFYRYIFDAECALEKLITDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSQSGYSEVNLSKLFADCAVWS

Target ORF information:

RefSeq Version	NM_001206065.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206065.1

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTCTGTG TCAGATTCCA GGAGTCTTGT AGCTTCCCGA 
TCCCGGAGAG AGAAGAAATC AAAGAAAGGG CGACAGCAAG CTCTGGAAAG ACTGAAAAGG 
GCAAAAGCTG GTGAGAAGTA TAAATATGAA GTAGAGGACT TCACGAGTGT TTATGAAGAA 
GTTGATGAGG AACAGTACTC AAAGCTGGTT CAGGCCCGCC AGGATGACGA CTGGATCGTG 
GATGACGATG GTATTGGCTA TGTGGAGGAT GGCCGGGAAA TTTTTGATGA TGACCTTGAA 
GATGATGCTC TTGATTCCCA TGAGAAAGGA AAAGATAATA AAGCATGCAA CAAAGATAAG 
AGGACTGTTA AGAGGGCTGC TGTGACAAAA CCGAACAACA TCAAATCAAT GTTCATTGCC 
AGTGCTGGGA GGAAAACTGT AGATAAAGCT GTTGACTTGT CCAAGGATGA TCTGTTAGGT 
GACATTCTAC AGGACCTGAA CACTGAGACA CCACAAATAA CTCCACCACC TGTAATAATA 
CCAAAAAAGA AAAGATCCAG TGGAGCTTCA CTGAATCCTT TCTCTGTGCA CACCCCCAAG 
GCAGCTCCTT CAGGAAAAAC CACTCCCCCA GCCTCAAGGA GAGAGCCTTC ATTAACTCCT 
GCTCCTCTTA CATGTGCTGA ATTTGCTGGA GAGCCCGGGC CGCCACTGTG TACAGATGAC 
AAGGAGGAGT CGGGAGCAAT GGAATTTGAA GATGGCGACT TTGATGAGCC CATGGAAGCT 
GAGGAGGTGG ATGTGGAGCC CGTGGCTGCC AAGACTTTGT GCCAAGAGAG GGAGCCAGCA 
GAGGAGGCAA AACACAAGGC TGATTCTGGG AAAGGGACCA CATCCTCCTC AGCAAGTTTT 
CTCCCAGATG TCTCTTGTTG GGACATGGAT CAGGAAGATG ACAGCAGTTT CTCAGCCCTG 
GAAGTTCAAG TGGATTCCAG TCACCTCCCA CTGGTAAAAG GAGCTGATGA GGAACAAGTA 
TTTCAGTTTT ATTGGCTGGA TGCTTATGAA GATCCGTACA GCCAACCAGG TGTGGTATTT 
CTGTTTGGCA AGGTCTGGAT CGAATCTGCT GAAACCCACG TGAGCTGTTG TGTCATGGTG 
AAAAACATTG AGCGATCGCT CTGTTTCCTT CCCCGTGAAA TGAAAGTTGA TCTAAATACG 
GGGAAAGAAA GTGGAACTCC AGTTACCATG AAGGATGTTT ATGATGAATT TGATGAGAAA 
ATAGCAGCAA AATATAAGAT TATGAAATTC AAGTCCAAGG TAGTGGAAAA GAACTATGCT 
TTTGAGATAC CTGATGTTCC GGAGAAATCT GAGTACTTGG AAGTTAGATA CTCGGCTGAA 
ATGCCACAGC TTCCTCAGGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC 
ACATCCAGCC TGGAACTGTT CTTAATGAAT AGAAAGATCA AAGGACCTTG TTGGCTTGAA 
GTGAAAAATC CACAGCTCTT GAATCAGCCA ATCAGTTGGT GTAAAGTTGA GGCAATGGTC 
TTGAAACCGG ACCTGGTGAA CGTAATTAAG GATGTTGGTC CTCCTCCAGT TGTGGTCATG 
TCTTTGAGCA TGAAGACAAT GCAGAATGCA AAGACACATG AAAATGAGAT TATTGCTGTG 
TCAGCGTTGG TCCATCACAG CTTTGCATTA GATAAAGCAC CCCCACAGCC TCCCTTTCAG 
TCACACTTCT GCGTTGTGTC TAAACCAAAG GACTGCATTT TTCCATATGA TTTCAAAGAA 
AGCATTAAAG AAAAGAATGT GAAGGTTGAG GTTGCTGCCA CGGAAAGAAC ACTGCTAGGT 
TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT 
GGTTTTGAAC TGGAAGTGCT ACTGCAGAGA ATTAATGTGT GCAAAGTTCC TTTCTGGTCC 
AAGATAGGTC GGCTGAAGCG ATCCAACATG CCGAAGCTTG GGGGCAGGAG TGGATTTGGT 
GAAAAAAATG CTACCTGTGG CCGAATGATC TGTGATGTGG AAATTTCGGC GAAGGAACTG 
ATTCGGTGTA AAAGCTACCA TTTATCTGAA CTTGTTCAAC AGATTTTGAA AACTGAAAGG 
ATTGTAATCC CAATAGAAAC TATACGAAAC ATGTACAGTG ACTCTTCTCA TCTGTTGTAC 
CTGTTGGAGC ACACCTGGAA AGATGCCAGG TTCATTTTGC AGATCATGTG TGAGCTAAAT 
GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC TAGGACACTG 
ATGGGTGGAC GATCCGAGCG TAATGAGTTC TTACTACTTC ATGCATTTTA TGAAAACAAC 
TATATTGTGC CTGACAAGCA GACTTTCAGA AAGCCTCAGC AGAAACTGGG AGATGAAGAT 
GAAGACATTG ATGGAGACAC CAATAAATAC AAAAAAGGAC GTAAGAAAGC AGCTTATTCT 
GGAGGCTTGG TCTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC 
TTCAACAGTT TGTATCCTTC CATCATTCAG GAATTCAACA TTTGTTTTAC AACAGTGCAA 
AGAGTTGCTT CAGAGGCACA GAAAGTTGTA GAGGATGGAG AGCAAGAGCA GATCCCCGAG 
CTGCCAGATC CAAGCTTGGA AATGGGCATT TTGCCCAGAG AGATCCGAAA ACTGGTAGAG 
CGGAGAAGAC AAGTAAAACA GCTAATGAAA CAGCAAGATT TAAATCCAGA CCTTCATCTT 
CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACGGCAA ACAGTATGTA CGGCTGCCTG 
GGATTCTCCT ACAGCAGATT TTACGCCAAA CCCCTGGCTG CTTTGGTGAC ATACAAAGGA 
AGGGAGATTT TGATGCATAC GAAGGAGATG GTTCAGAAGA TGAATCTTGA AGTTATTTAT 
GGAGATACAG ATTCAATTAT GATCAACACC AATAGCACCA ATCTGGAAGA AGTGTTTAAA 
TTGGGAAACA AGGTGAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT 
GATGGTATTT TCAAGTCCCT GCTACTGCTG AAGAAAAAAA AGTATGCTGC TCTGATTGTT 
GAGCCAACAT CAGATGGGAA TTACGTCACG AAACAGGAGG TGAAAGGATT AGATATAGTT 
AGAAGAGACT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTCATTGG CCAGATTCTT 
TCGGATCAAA ACCGGGACAC TATAGTGGAA AACATTCAGA AGAGGTTAAT AGAAATTGGA 
GAAAATGTGC TAAACGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAATAA GGCGTTGACC 
AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG 
ATAAATTCTC AAGGAGGAAG AAAGGTGAAA GCTGGAGATA CTGTGTCCTA TGTCATCTGT 
CAGGATGGTT CCAATCTCAC TGCGGGTCAG AGGGCCTATG CACCCGAGCA GCTGCAGAAG 
CAGGACAATT TAAGCATTGA TACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG 
GCTCGAATAT GTGAGCCAAT AGATGGAATT GATGCTATCC TCATTGCATC CTGGTTAGGA 
CTTGACCCCA CCCAATTTAG GGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTTTA 
CTTGGTGGCC CAGCACACCT CACGGATGAA GAGAAATACA AGGACTGTGA AAGGTTCAAA 
TGCCCATGCC CTACATGTGG AACCGAGAAT ATTTATGATA GTGTCTTAGA TGGTTCGGGA 
ACTGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCA ATTGTAAGGC TTCTCCTCTG 
ACCTTTATGG TACAGCTGAG CAACAAATTG ATCATGGACA TTAGACGTTG CATTAAAAAA 
TACTATGAAG GCTGGTTGAT TTGTGAAGAG CCCACATGTC GAAATCGAAC TCGGCACCTT 
CCCCTTCAGT TCTCTCGAAA TGGACCCCTT TGCCAAGTCT GCATGAAAGC TACACTTCGA 
CTAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT 
GCGGAGTGTG CACTGGAGAA ACTTATTACC GATCATGAGA AAGATAAATT GAAGAAGCAG 
TTTTTTACTC CCAAAGTTCT TCAGGACTAT AGAAAACTCA AGAACACAGC AGAGCAGTTC 
TTGTCCCAAA GTGGCTATTC CGAAGTGAAC CTCAGCAAAC TCTTCGCCGA TTGTGCTGTA 
TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_005228356.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228413.1 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35737
Clone ID Related Accession (Same CDS sequence)	XM_005228356.3 , XM_005228356.4
Accession Version	XM_005228356.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA 
GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG 
AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC 
TTCGCCGATT GTGCTGTATG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228413.1
CDS	175..4581
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEYSDKSLYTQLCFYRYIFDAECALEKLITDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSQSGYSEVNLSKLFADCAVWS

Target ORF information:

RefSeq Version	XM_005228356.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228356.3

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA 
GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG 
AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC 
TTCGCCGATT GTGCTGTATG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_005228356.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228413.1 Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

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CloneID	OBa35737
Clone ID Related Accession (Same CDS sequence)	XM_005228356.3 , XM_005228356.4
Accession Version	XM_005228356.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228356.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA 
GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG 
AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC 
TTCGCCGATT GTGCTGTATG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228413.1
CDS	196..4602
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEYSDKSLYTQLCFYRYIFDAECALEKLITDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSQSGYSEVNLSKLFADCAVWS

Target ORF information:

RefSeq Version	XM_005228356.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228356.4

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTATTACCGA TCATGAGAAA 
GATAAATTGA AGAAGCAGTT TTTTACTCCC AAAGTTCTTC AGGACTATAG AAAACTCAAG 
AACACAGCAG AGCAGTTCTT GTCCCAAAGT GGCTATTCCG AAGTGAACCT CAGCAAACTC 
TTCGCCGATT GTGCTGTATG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_005228359.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228416.1 Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

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CloneID	OBa35739
Clone ID Related Accession (Same CDS sequence)	XM_005228359.4 , XM_005228359.3
Accession Version	XM_005228359.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA 
AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA 
ACTGCATCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228416.1
CDS	196..4470
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEEDVLLFLTLLVEKLLRSELAQSQKGEEQGSEPTASE

Target ORF information:

RefSeq Version	XM_005228359.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228359.4

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA 
AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA 
ACTGCATCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_005228359.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228416.1 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35739
Clone ID Related Accession (Same CDS sequence)	XM_005228359.4 , XM_005228359.3
Accession Version	XM_005228359.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA 
AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA 
ACTGCATCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228416.1
CDS	176..4450
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEEDVLLFLTLLVEKLLRSELAQSQKGEEQGSEPTASE

Target ORF information:

RefSeq Version	XM_005228359.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228359.3

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGGAAGAT GTGTTACTTT TTCTCACTTT ACTGGTTGAA 
AAACTGCTGA GAAGTGAACT GGCCCAAAGC CAGAAAGGGG AAGAACAAGG AAGTGAACCA 
ACTGCATCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_005228360.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228417.1 Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa35740
Clone ID Related Accession (Same CDS sequence)	XM_005228360.3 , XM_005228360.4
Accession Version	XM_005228360.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT 
GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228417.1
CDS	176..4414
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEMKKLRLGEVAHPGTLPPINVTARI

Target ORF information:

RefSeq Version	XM_005228360.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228360.3

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT 
GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_005228360.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228417.1 Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

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CloneID	OBa35740
Clone ID Related Accession (Same CDS sequence)	XM_005228360.3 , XM_005228360.4
Accession Version	XM_005228360.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA polymerase alpha catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228360.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT 
GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228417.1
CDS	196..4434
Translation	MAPLHGEDCEPAANAVSDSRSLVASRSRREKKSKKGRQQALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDNKACNKDKRTVKRAAVTKPNNIKSMFIASAGRKTVDKAVDLSKDDLLGDILQDLNTETPQITPPPVIIPKKKRSSGASLNPFSVHTPKAAPSGKTTPPASRREPSLTPAPLTCAEFAGEPGPPLCTDDKEESGAMEFEDGDFDEPMEAEEVDVEPVAAKTLCQEREPAEEAKHKADSGKGTTSSSASFLPDVSCWDMDQEDDSSFSALEVQVDSSHLPLVKGADEEQVFQFYWLDAYEDPYSQPGVVFLFGKVWIESAETHVSCCVMVKNIERSLCFLPREMKVDLNTGKESGTPVTMKDVYDEFDEKIAAKYKIMKFKSKVVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKVEAMVLKPDLVNVIKDVGPPPVVVMSLSMKTMQNAKTHENEIIAVSALVHHSFALDKAPPQPPFQSHFCVVSKPKDCIFPYDFKESIKEKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPFWSKIGRLKRSNMPKLGGRSGFGEKNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERIVIPIETIRNMYSDSSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQTFRKPQQKLGDEDEDIDGDTNKYKKGRKKAAYSGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVVEDGEQEQIPELPDPSLEMGILPREIRKLVERRRQVKQLMKQQDLNPDLHLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGIFKSLLLLKKKKYAALIVEPTSDGNYVTKQEVKGLDIVRRDWCDLAKDTGNFVIGQILSDQNRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTAGQRAYAPEQLQKQDNLSIDTQYYLAQQIHPVVARICEPIDGIDAILIASWLGLDPTQFRVHHYHKDEENDALLGGPAHLTDEEKYKDCERFKCPCPTCGTENIYDSVLDGSGTDMEPSLYRCSNINCKASPLTFMVQLSNKLIMDIRRCIKKYYEGWLICEEPTCRNRTRHLPLQFSRNGPLCQVCMKATLRLEMKKLRLGEVAHPGTLPPINVTARI

Target ORF information:

RefSeq Version	XM_005228360.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228360.4

ORF Insert Sequence:

ATGGCGCCCT TGCATGGCGA GGATTGCGAG CCCGCGGCGA ACGCTGTGTC AGATTCCAGG 
AGTCTTGTAG CTTCCCGATC CCGGAGAGAG AAGAAATCAA AGAAAGGGCG ACAGCAAGCT 
CTGGAAAGAC TGAAAAGGGC AAAAGCTGGT GAGAAGTATA AATATGAAGT AGAGGACTTC 
ACGAGTGTTT ATGAAGAAGT TGATGAGGAA CAGTACTCAA AGCTGGTTCA GGCCCGCCAG 
GATGACGACT GGATCGTGGA TGACGATGGT ATTGGCTATG TGGAGGATGG CCGGGAAATT 
TTTGATGATG ACCTTGAAGA TGATGCTCTT GATTCCCATG AGAAAGGAAA AGATAATAAA 
GCATGCAACA AAGATAAGAG GACTGTTAAG AGGGCTGCTG TGACAAAACC GAACAACATC 
AAATCAATGT TCATTGCCAG TGCTGGGAGG AAAACTGTAG ATAAAGCTGT TGACTTGTCC 
AAGGATGATC TGTTAGGTGA CATTCTACAG GACCTGAACA CTGAGACACC ACAAATAACT 
CCACCACCTG TAATAATACC AAAAAAGAAA AGATCCAGTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGCTCCTTCA GGAAAAACCA CTCCCCCAGC CTCAAGGAGA 
GAGCCTTCAT TAACTCCTGC TCCTCTTACA TGTGCTGAAT TTGCTGGAGA GCCCGGGCCG 
CCACTGTGTA CAGATGACAA GGAGGAGTCG GGAGCAATGG AATTTGAAGA TGGCGACTTT 
GATGAGCCCA TGGAAGCTGA GGAGGTGGAT GTGGAGCCCG TGGCTGCCAA GACTTTGTGC 
CAAGAGAGGG AGCCAGCAGA GGAGGCAAAA CACAAGGCTG ATTCTGGGAA AGGGACCACA 
TCCTCCTCAG CAAGTTTTCT CCCAGATGTC TCTTGTTGGG ACATGGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCTGGA AGTTCAAGTG GATTCCAGTC ACCTCCCACT GGTAAAAGGA 
GCTGATGAGG AACAAGTATT TCAGTTTTAT TGGCTGGATG CTTATGAAGA TCCGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAG GTCTGGATCG AATCTGCTGA AACCCACGTG 
AGCTGTTGTG TCATGGTGAA AAACATTGAG CGATCGCTCT GTTTCCTTCC CCGTGAAATG 
AAAGTTGATC TAAATACGGG GAAAGAAAGT GGAACTCCAG TTACCATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAGATTA TGAAATTCAA GTCCAAGGTA 
GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCGG AGAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTATTTG GGACCAACAC ATCCAGCCTG GAACTGTTCT TAATGAATAG AAAGATCAAA 
GGACCTTGTT GGCTTGAAGT GAAAAATCCA CAGCTCTTGA ATCAGCCAAT CAGTTGGTGT 
AAAGTTGAGG CAATGGTCTT GAAACCGGAC CTGGTGAACG TAATTAAGGA TGTTGGTCCT 
CCTCCAGTTG TGGTCATGTC TTTGAGCATG AAGACAATGC AGAATGCAAA GACACATGAA 
AATGAGATTA TTGCTGTGTC AGCGTTGGTC CATCACAGCT TTGCATTAGA TAAAGCACCC 
CCACAGCCTC CCTTTCAGTC ACACTTCTGC GTTGTGTCTA AACCAAAGGA CTGCATTTTT 
CCATATGATT TCAAAGAAAG CATTAAAGAA AAGAATGTGA AGGTTGAGGT TGCTGCCACG 
GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG TTTTGAACTG GAAGTGCTAC TGCAGAGAAT TAATGTGTGC 
AAAGTTCCTT TCTGGTCCAA GATAGGTCGG CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAAAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCGGCGA AGGAACTGAT TCGGTGTAAA AGCTACCATT TATCTGAACT TGTTCAACAG 
ATTTTGAAAA CTGAAAGGAT TGTAATCCCA ATAGAAACTA TACGAAACAT GTACAGTGAC 
TCTTCTCATC TGTTGTACCT GTTGGAGCAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACACTGAT GGGTGGACGA TCCGAGCGTA ATGAGTTCTT ACTACTTCAT 
GCATTTTATG AAAACAACTA TATTGTGCCT GACAAGCAGA CTTTCAGAAA GCCTCAGCAG 
AAACTGGGAG ATGAAGATGA AGACATTGAT GGAGACACCA ATAAATACAA AAAAGGACGT 
AAGAAAGCAG CTTATTCTGG AGGCTTGGTC TTGGACCCCA AAGTTGGTTT TTATGATAAG 
TTCATTTTGC TTCTGGACTT CAACAGTTTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGGCACAGA AAGTTGTAGA GGATGGAGAG 
CAAGAGCAGA TCCCCGAGCT GCCAGATCCA AGCTTGGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGAAAAC TGGTAGAGCG GAGAAGACAA GTAAAACAGC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTCATCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACGGCAAAC 
AGTATGTACG GCTGCCTGGG ATTCTCCTAC AGCAGATTTT ACGCCAAACC CCTGGCTGCT 
TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AGGAGATGGT TCAGAAGATG 
AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TCAACACCAA TAGCACCAAT 
CTGGAAGAAG TGTTTAAATT GGGAAACAAG GTGAAAAGTG AAGTGAATAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA TGGTATTTTC AAGTCCCTGC TACTGCTGAA GAAAAAAAAG 
TATGCTGCTC TGATTGTTGA GCCAACATCA GATGGGAATT ACGTCACGAA ACAGGAGGTG 
AAAGGATTAG ATATAGTTAG AAGAGACTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC GGATCAAAAC CGGGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AACGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAATAAGG CGTTGACCAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT 
GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGAAGAA AGGTGAAAGC TGGAGATACT 
GTGTCCTATG TCATCTGTCA GGATGGTTCC AATCTCACTG CGGGTCAGAG GGCCTATGCA 
CCCGAGCAGC TGCAGAAGCA GGACAATTTA AGCATTGATA CCCAGTACTA CCTGGCCCAG 
CAGATCCACC CAGTCGTGGC TCGAATATGT GAGCCAATAG ATGGAATTGA TGCTATCCTC 
ATTGCATCCT GGTTAGGACT TGACCCCACC CAATTTAGGG TTCATCATTA TCATAAAGAT 
GAAGAGAATG ATGCTTTACT TGGTGGCCCA GCACACCTCA CGGATGAAGA GAAATACAAG 
GACTGTGAAA GGTTCAAATG CCCATGCCCT ACATGTGGAA CCGAGAATAT TTATGATAGT 
GTCTTAGATG GTTCGGGAAC TGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCAAT 
TGTAAGGCTT CTCCTCTGAC CTTTATGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGC TGGTTGATTT GTGAAGAGCC CACATGTCGA 
AATCGAACTC GGCACCTTCC CCTTCAGTTC TCTCGAAATG GACCCCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGACT AGAGATGAAG AAACTGAGGC TTGGAGAGGT TGCTCATCCT 
GGGACTTTAC CTCCAATAAA TGTCACAGCC AGGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	POLA1
Entrez Gene ID	534848
Full Name	DNA polymerase alpha 1, catalytic subunit
General protein information	Preferred Names DNA polymerase alpha 1, catalytic subunit Names DNA polymerase alpha catalytic subunit polymerase (DNA directed), alpha 1, catalytic subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

POLA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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