HECTD1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa228467 XM_024982055.1
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa228468 XM_024982056.1
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa31014 XM_002696696.6
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31014 XM_015468539.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31014 XM_005222071.4
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31014 XM_872366.7
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31015 XM_005222072.4
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31015 XM_005196278.3
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31016 XM_005222073.4
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31016 XM_005196279.3
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31017 XM_005222074.4
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31017 XM_005196277.3
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31018 XM_005222075.4
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa31018 XM_015468540.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa31014 XM_002696696.5 Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31016 XM_005222073.3 Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31014 XM_005222071.3 Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31017 XM_005222074.3 Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31015 XM_005222072.3 Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31018 XM_005222075.3 Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa228467
    Clone ID Related Accession (Same CDS sequence) XM_024982055.1
    Accession Version XM_024982055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGAT TTTTGTGAGC ATGGAACAAA GTCTGGGTTA
    AACCAGGGGG CCATTTCTAC TCTTCAAAGT AGTGATATTC TGAATTTGAC AAAAGAACAA
    CCTCAGGCCA AAGCAGGCAA TGGGCAGAAT TCATGTGGCG TGGAAGACGT CCTTCAGCTC
    CTGCGTATTC TGTATATAGT TGCAAGTGAC CCTTACTCAA GAATATCCCA GGAAGAAGGT
    GATGAGCAGC CTCAGTTTAC TTTTCCACCA GATGAATTCA CTAGCAAAAA AATCACAACA
    AAAATTTTGC AGCAAATTGA GGAACCGTTG GCATTGGCAA GTGGAGCTCT GCCAGACTGG
    TGTGAACAGT TAACCAGCAA GTGTCCTTTT CTAATACCGT TCGAAACTAG ACAGCTTTAT
    TTCACATGTA CAGCATTTGG TGCATCAAGA GCAATTGTGT GGCTACAAAA CCGACGTGAG
    GCCACTGTGG AAAGAACAAG GACCACAAGT AGTGTGAGGC GAGATGATCC TGGAGAGTTC
    CGTGTTGGTC GTCTCAAGCA CGAAAGAGTA AAAGTTCCAC GTGGTGAATC ACTGATGGAG
    TGGGCCGAGA ATGTCATGCA AATACACGCA GATCGGAAAT CAGTGCTTGA GGTTGAATTT
    TTAGGAGAAG AAGGAACTGG TTTGGGGCCC ACCTTAGAGT TCTATGCTCT GGTAGCAGCA
    GAATTCCAGA GAACTGACTT GGGAGCTTGG CTTTGTGATG ACAACTTTCC AGATGATGAA
    TCTCGTCATG TCGATCTTGG AGGTGGATTG AAACCTCCCG GATACTATGT GCAGAGATCA
    TGTGGACTGT TTACGGCTCC ATTTCCACAA GACAGTGACG AGCTTGAAAG GATCACAAAA
    CTCTTTCATT TCCTTGGAAT TTTCTTGGCC AAATGCATTC AAGACAATAG ACTTGTGGAC
    TTACCTATTT CCAAACCTTT CTTTAAACTT ATGTGTATGG GGGACATTAA AAGTAATATG
    AGCAAACTGA TTTATGAGTC ACGAGGCGAC AGAGACCTAC ACTGCACTGA AAGTCAGTCT
    GAAGCCTCTA CAGAAGAAGG GCATGACTCA CTCTCAGTAG GAAGCTTTGA GGAGGATTCA
    AAATCAGAAT TCATTCTAGA TCCCCCCAAA CCAAAACCGC CAGCTTGGTT TAATGGAATT
    TTAACTTGGG AAGACTTTGA ACTAGTAAAC CCACACAGAG CCAGATTTTT AAAAGAAATT
    AAAGACCTTG CCATCAAGAG GCGCCAGATT TTAAGCAACA AAGGTCTTTC TGAAGACGAG
    AAGAACACAA AATTACAAGA ACTAGTGCTG AAGAATCCAT CAGGTTCTGG GCCTCCACTT
    AGCATAGAGG ATTTAGGTTT AAATTTCCAG TTTTGCCCTT CCTCAAGAAT ATATGGTTTT
    ACAGCTGTGG ATCTCAAGCC AAGTGGTGAA GATGAGATGA TAACAATGGA TAATGCAGAA
    GAATATGTGG ATTTGATGTT TGACTTTTGC ATGCATACGG GTATTCAGAA ACAGATGGAA
    GCCTTCAGAG ATGGGTTTAA TAAAGTTTTT CCAATGGAGA AATTAAGTTC CTTCAGCCAT
    GAAGAGGTCC AAATGATTCT TTGTGGAAAT CAGTCACCAT CCTGGGCAGC AGAGGATATT
    ATTAATTATA CTGAACCTAA GTTGGGTTAT ACACGTGACA GTCCTGGCTT CCTTAGGTTC
    GTGCGGGTTT TATGTGGTAT GTCTTCAGAC GAGAGGAAAG CATTTCTGCA GTTTACCACT
    GGTTGTTCAA CTCTGCCTCC AGGGGGACTG GCTAACTTGC ATCCCAGGCT CACAGTTGTA
    CGCAAGGTTG ATGCTACTGA TGCAAGCTAC CCATCAGTGA ACACATGTGT GCATTATCTC
    AAGTTGCCAG AATACTCTTC GGAGGAGATC ATGAGAGAGC GTCTGCTAGC TGCTACAATG
    GAGAAAGGCT TTCATCTCAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837823.1
    CDS610..8253
    Translation

    Target ORF information:

    RefSeq Version XM_024982055.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGAT TTTTGTGAGC ATGGAACAAA GTCTGGGTTA
    AACCAGGGGG CCATTTCTAC TCTTCAAAGT AGTGATATTC TGAATTTGAC AAAAGAACAA
    CCTCAGGCCA AAGCAGGCAA TGGGCAGAAT TCATGTGGCG TGGAAGACGT CCTTCAGCTC
    CTGCGTATTC TGTATATAGT TGCAAGTGAC CCTTACTCAA GAATATCCCA GGAAGAAGGT
    GATGAGCAGC CTCAGTTTAC TTTTCCACCA GATGAATTCA CTAGCAAAAA AATCACAACA
    AAAATTTTGC AGCAAATTGA GGAACCGTTG GCATTGGCAA GTGGAGCTCT GCCAGACTGG
    TGTGAACAGT TAACCAGCAA GTGTCCTTTT CTAATACCGT TCGAAACTAG ACAGCTTTAT
    TTCACATGTA CAGCATTTGG TGCATCAAGA GCAATTGTGT GGCTACAAAA CCGACGTGAG
    GCCACTGTGG AAAGAACAAG GACCACAAGT AGTGTGAGGC GAGATGATCC TGGAGAGTTC
    CGTGTTGGTC GTCTCAAGCA CGAAAGAGTA AAAGTTCCAC GTGGTGAATC ACTGATGGAG
    TGGGCCGAGA ATGTCATGCA AATACACGCA GATCGGAAAT CAGTGCTTGA GGTTGAATTT
    TTAGGAGAAG AAGGAACTGG TTTGGGGCCC ACCTTAGAGT TCTATGCTCT GGTAGCAGCA
    GAATTCCAGA GAACTGACTT GGGAGCTTGG CTTTGTGATG ACAACTTTCC AGATGATGAA
    TCTCGTCATG TCGATCTTGG AGGTGGATTG AAACCTCCCG GATACTATGT GCAGAGATCA
    TGTGGACTGT TTACGGCTCC ATTTCCACAA GACAGTGACG AGCTTGAAAG GATCACAAAA
    CTCTTTCATT TCCTTGGAAT TTTCTTGGCC AAATGCATTC AAGACAATAG ACTTGTGGAC
    TTACCTATTT CCAAACCTTT CTTTAAACTT ATGTGTATGG GGGACATTAA AAGTAATATG
    AGCAAACTGA TTTATGAGTC ACGAGGCGAC AGAGACCTAC ACTGCACTGA AAGTCAGTCT
    GAAGCCTCTA CAGAAGAAGG GCATGACTCA CTCTCAGTAG GAAGCTTTGA GGAGGATTCA
    AAATCAGAAT TCATTCTAGA TCCCCCCAAA CCAAAACCGC CAGCTTGGTT TAATGGAATT
    TTAACTTGGG AAGACTTTGA ACTAGTAAAC CCACACAGAG CCAGATTTTT AAAAGAAATT
    AAAGACCTTG CCATCAAGAG GCGCCAGATT TTAAGCAACA AAGGTCTTTC TGAAGACGAG
    AAGAACACAA AATTACAAGA ACTAGTGCTG AAGAATCCAT CAGGTTCTGG GCCTCCACTT
    AGCATAGAGG ATTTAGGTTT AAATTTCCAG TTTTGCCCTT CCTCAAGAAT ATATGGTTTT
    ACAGCTGTGG ATCTCAAGCC AAGTGGTGAA GATGAGATGA TAACAATGGA TAATGCAGAA
    GAATATGTGG ATTTGATGTT TGACTTTTGC ATGCATACGG GTATTCAGAA ACAGATGGAA
    GCCTTCAGAG ATGGGTTTAA TAAAGTTTTT CCAATGGAGA AATTAAGTTC CTTCAGCCAT
    GAAGAGGTCC AAATGATTCT TTGTGGAAAT CAGTCACCAT CCTGGGCAGC AGAGGATATT
    ATTAATTATA CTGAACCTAA GTTGGGTTAT ACACGTGACA GTCCTGGCTT CCTTAGGTTC
    GTGCGGGTTT TATGTGGTAT GTCTTCAGAC GAGAGGAAAG CATTTCTGCA GTTTACCACT
    GGTTGTTCAA CTCTGCCTCC AGGGGGACTG GCTAACTTGC ATCCCAGGCT CACAGTTGTA
    CGCAAGGTTG ATGCTACTGA TGCAAGCTAC CCATCAGTGA ACACATGTGT GCATTATCTC
    AAGTTGCCAG AATACTCTTC GGAGGAGATC ATGAGAGAGC GTCTGCTAGC TGCTACAATG
    GAGAAAGGCT TTCATCTCAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228468
    Clone ID Related Accession (Same CDS sequence) XM_024982056.1
    Accession Version XM_024982056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837824.1
    CDS610..7422
    Translation

    Target ORF information:

    RefSeq Version XM_024982056.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31014
    Clone ID Related Accession (Same CDS sequence) XM_002696696.6 , XM_005222071.3 , XM_002696696.5 , XM_005222071.4 , XM_015468539.1 , XM_872366.7
    Accession Version XM_002696696.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696696.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696742.1
    CDS351..8183
    Translation

    Target ORF information:

    RefSeq Version XM_002696696.6
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696696.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31014
    Clone ID Related Accession (Same CDS sequence) XM_002696696.6 , XM_005222071.3 , XM_002696696.5 , XM_005222071.4 , XM_015468539.1 , XM_872366.7
    Accession Version XM_005222071.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222071.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222128.1
    CDS314..8146
    Translation

    Target ORF information:

    RefSeq Version XM_005222071.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222071.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31014
    Clone ID Related Accession (Same CDS sequence) XM_002696696.6 , XM_005222071.3 , XM_002696696.5 , XM_005222071.4 , XM_015468539.1 , XM_872366.7
    Accession Version XM_002696696.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696696.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696742.1
    CDS125..7957
    Translation

    Target ORF information:

    RefSeq Version XM_002696696.5
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696696.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31014
    Clone ID Related Accession (Same CDS sequence) XM_002696696.6 , XM_005222071.3 , XM_002696696.5 , XM_005222071.4 , XM_015468539.1 , XM_872366.7
    Accession Version XM_005222071.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222071.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222128.1
    CDS610..8442
    Translation

    Target ORF information:

    RefSeq Version XM_005222071.4
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222071.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31014
    Clone ID Related Accession (Same CDS sequence) XM_002696696.6 , XM_005222071.3 , XM_002696696.5 , XM_005222071.4 , XM_015468539.1 , XM_872366.7
    Accession Version XM_015468539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324025.1
    CDS314..8146
    Translation

    Target ORF information:

    RefSeq Version XM_015468539.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31014
    Clone ID Related Accession (Same CDS sequence) XM_002696696.6 , XM_005222071.3 , XM_002696696.5 , XM_005222071.4 , XM_015468539.1 , XM_872366.7
    Accession Version XM_872366.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_872366.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_877459.5
    CDS125..7957
    Translation

    Target ORF information:

    RefSeq Version XM_872366.7
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_872366.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCGTTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAGAA TGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT TTGGGGCCCA CCTTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG
    CAGAGATCAT GTGGACTGTT TACGGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG TCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTCA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31015
    Clone ID Related Accession (Same CDS sequence) XM_005222072.3 , XM_005196278.3 , XM_005222072.4
    Accession Version XM_005222072.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCG TTGGCATTGG CAAGTGGAGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AGAATGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTTTGGGG CCCACCTTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA
    TCATGTGGAC TGTTTACGGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGTCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTCAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222129.1
    CDS314..8140
    Translation

    Target ORF information:

    RefSeq Version XM_005222072.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222072.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCG TTGGCATTGG CAAGTGGAGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AGAATGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTTTGGGG CCCACCTTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA
    TCATGTGGAC TGTTTACGGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGTCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTCAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31015
    Clone ID Related Accession (Same CDS sequence) XM_005222072.3 , XM_005196278.3 , XM_005222072.4
    Accession Version XM_005196278.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCG TTGGCATTGG CAAGTGGAGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AGAATGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTTTGGGG CCCACCTTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA
    TCATGTGGAC TGTTTACGGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGTCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTCAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005196335.2
    CDS314..8140
    Translation

    Target ORF information:

    RefSeq Version XM_005196278.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005196278.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCG TTGGCATTGG CAAGTGGAGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AGAATGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTTTGGGG CCCACCTTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA
    TCATGTGGAC TGTTTACGGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGTCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTCAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31015
    Clone ID Related Accession (Same CDS sequence) XM_005222072.3 , XM_005196278.3 , XM_005222072.4
    Accession Version XM_005222072.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222072.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCG TTGGCATTGG CAAGTGGAGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AGAATGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTTTGGGG CCCACCTTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA
    TCATGTGGAC TGTTTACGGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGTCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTCAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222129.1
    CDS610..8436
    Translation

    Target ORF information:

    RefSeq Version XM_005222072.4
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222072.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACTAAGGGGG GCCGCCGGAG AACATGGGAT
    GATGATTACG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCG TTGGCATTGG CAAGTGGAGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AGAATGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTTTGGGG CCCACCTTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA
    TCATGTGGAC TGTTTACGGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGTCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTCAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31016
    Clone ID Related Accession (Same CDS sequence) XM_005196279.3 , XM_005222073.4 , XM_005222073.3
    Accession Version XM_005196279.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196279.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC GCAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAACGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTGGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTGG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACTAA GGGGGGCCGC CGGAGAACAT GGGATGATGA TTACGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCGTTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAGAAT
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTT TGGGGCCCAC CTTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGACTGTTT
    ACGGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGT CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTCAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005196336.2
    CDS314..8005
    Translation

    Target ORF information:

    RefSeq Version XM_005196279.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005196279.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC GCAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAACGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTGGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTGG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACTAA GGGGGGCCGC CGGAGAACAT GGGATGATGA TTACGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCGTTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAGAAT
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTT TGGGGCCCAC CTTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGACTGTTT
    ACGGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGT CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTCAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31016
    Clone ID Related Accession (Same CDS sequence) XM_005196279.3 , XM_005222073.4 , XM_005222073.3
    Accession Version XM_005222073.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222073.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC GCAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAACGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTGGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTGG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACTAA GGGGGGCCGC CGGAGAACAT GGGATGATGA TTACGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCGTTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAGAAT
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTT TGGGGCCCAC CTTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGACTGTTT
    ACGGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGT CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTCAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222130.1
    CDS610..8301
    Translation

    Target ORF information:

    RefSeq Version XM_005222073.4
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222073.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC GCAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAACGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTGGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTGG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACTAA GGGGGGCCGC CGGAGAACAT GGGATGATGA TTACGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCGTTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAGAAT
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTT TGGGGCCCAC CTTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGACTGTTT
    ACGGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGT CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTCAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31016
    Clone ID Related Accession (Same CDS sequence) XM_005196279.3 , XM_005222073.4 , XM_005222073.3
    Accession Version XM_005222073.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC GCAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAACGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTGGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTGG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACTAA GGGGGGCCGC CGGAGAACAT GGGATGATGA TTACGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCGTTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAGAAT
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTT TGGGGCCCAC CTTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGACTGTTT
    ACGGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGT CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTCAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222130.1
    CDS314..8005
    Translation

    Target ORF information:

    RefSeq Version XM_005222073.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222073.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC GCAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAACGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTGGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTGG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACTAA GGGGGGCCGC CGGAGAACAT GGGATGATGA TTACGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCGTTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAGAAT
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTT TGGGGCCCAC CTTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GAGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGACTGTTT
    ACGGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGT CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTCAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31017
    Clone ID Related Accession (Same CDS sequence) XM_005222074.4 , XM_005222074.3 , XM_005196277.3
    Accession Version XM_005222074.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222074.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCGTTGGCAT TGGCAAGTGG AGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAGAATGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTTTG GGGCCCACCT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG
    AGATCATGTG GACTGTTTAC GGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGTCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTCAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222131.1
    CDS610..8259
    Translation

    Target ORF information:

    RefSeq Version XM_005222074.4
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222074.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCGTTGGCAT TGGCAAGTGG AGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAGAATGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTTTG GGGCCCACCT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG
    AGATCATGTG GACTGTTTAC GGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGTCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTCAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31017
    Clone ID Related Accession (Same CDS sequence) XM_005222074.4 , XM_005222074.3 , XM_005196277.3
    Accession Version XM_005222074.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCGTTGGCAT TGGCAAGTGG AGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAGAATGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTTTG GGGCCCACCT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG
    AGATCATGTG GACTGTTTAC GGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGTCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTCAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222131.1
    CDS314..7963
    Translation

    Target ORF information:

    RefSeq Version XM_005222074.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222074.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCGTTGGCAT TGGCAAGTGG AGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAGAATGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTTTG GGGCCCACCT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG
    AGATCATGTG GACTGTTTAC GGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGTCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTCAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31017
    Clone ID Related Accession (Same CDS sequence) XM_005222074.4 , XM_005222074.3 , XM_005196277.3
    Accession Version XM_005196277.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196277.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
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    2881
    2941
    3001
    3061
    3121
    3181
    3241
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    4141
    4201
    4261
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    4381
    4441
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    4681
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    4921
    4981
    5041
    5101
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCGTTGGCAT TGGCAAGTGG AGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAGAATGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTTTG GGGCCCACCT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG
    AGATCATGTG GACTGTTTAC GGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGTCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTCAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005196334.2
    CDS314..7963
    Translation

    Target ORF information:

    RefSeq Version XM_005196277.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005196277.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CGCAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAACGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTGG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTG GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACTA AGGGGGGCCG CCGGAGAACA
    TGGGATGATG ATTACGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCGTTGGCAT TGGCAAGTGG AGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAGAATGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTTTG GGGCCCACCT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG
    AGATCATGTG GACTGTTTAC GGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGTCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTCAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31018
    Clone ID Related Accession (Same CDS sequence) XM_005222075.3 , XM_015468540.1 , XM_005222075.4
    Accession Version XM_005222075.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCGT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA GAATGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTTTGGGGC CCACCTTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT
    GTGCAGAGAT CATGTGGACT GTTTACGGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGTCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TCAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222132.1
    CDS313..7308
    Translation

    Target ORF information:

    RefSeq Version XM_005222075.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222075.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCGT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA GAATGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTTTGGGGC CCACCTTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT
    GTGCAGAGAT CATGTGGACT GTTTACGGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGTCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TCAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31018
    Clone ID Related Accession (Same CDS sequence) XM_005222075.3 , XM_015468540.1 , XM_005222075.4
    Accession Version XM_015468540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646535.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCGT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA GAATGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTTTGGGGC CCACCTTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT
    GTGCAGAGAT CATGTGGACT GTTTACGGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGTCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TCAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324026.1
    CDS313..7308
    Translation

    Target ORF information:

    RefSeq Version XM_015468540.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCGT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA GAATGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTTTGGGGC CCACCTTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT
    GTGCAGAGAT CATGTGGACT GTTTACGGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGTCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TCAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31018
    Clone ID Related Accession (Same CDS sequence) XM_005222075.3 , XM_015468540.1 , XM_005222075.4
    Accession Version XM_005222075.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222075.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCGT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA GAATGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTTTGGGGC CCACCTTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT
    GTGCAGAGAT CATGTGGACT GTTTACGGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGTCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TCAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222132.1
    CDS610..7605
    Translation

    Target ORF information:

    RefSeq Version XM_005222075.4
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222075.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGCCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATA
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTGGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCATAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCT TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAGAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT GTTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC GCCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AGGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGGTTAAGA
    TTCCGGTTGG AACGCGCACC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTGGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACG
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TGGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTGGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CTAAGGGGGG CCGCCGGAGA
    ACATGGGATG ATGATTACGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCGT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA GAATGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTTTGGGGC CCACCTTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT
    GTGCAGAGAT CATGTGGACT GTTTACGGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCACC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGTCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TCAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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