SOS1 cDNA ORF clone, Bos taurus(cattle)

The following SOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa06855 XM_005212708.4
Latest version!
Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06855 XM_005193883.3
Latest version!
Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06856 XM_005212709.4
Latest version!
Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06856 XM_005193884.3
Latest version!
Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06854 XM_617859.8
Latest version!
Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa06854 XM_002691241.6
Latest version!
Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa06854 XM_002691241.5 Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa06855 XM_005212708.3 Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06856 XM_005212709.3 Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa06855
    Clone ID Related Accession (Same CDS sequence) XM_005212708.3 , XM_005193883.3 , XM_005212708.4
    Accession Version XM_005212708.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA
    TCATCTATAA GTTTAACCAA GAGCACTGAT GAAGTGCCTG TCCCCCCTCC TGTTCCTCCA
    CGAAGACGGC CAGAGTCTGC CCCAGCGGAA TCTTCACCAT CTAAGATGAT GTCTAAGCAT
    TTGGACAGCC CCCCAGCAAT TCCTCCTAGG CAACCCACAT CAAAAGCCTA TTCACCACGG
    TATTCAATAT CAGACCGGAC CTCTATATCA GACCCTCCTG AAAGCCCTCC CTTATTACCT
    CCACGAGAAC CTGTGAGGAC ACCTGATGTT TTCTCAAGTT CACCACTACA TCTCCAACCT
    CCCCCTCTGG GCAAAAAAAG TGACCATAGT AATGCCTTCT TCCCAAACAG CCCCTCCCCC
    TTTACACCAC CTCCTCCTCA AACACCTTCT CCTCATGGAA CAAGAAGGCA TCTGCCGTCA
    CCACCACTGA CACAAGAAGT GGACCTCCAT TCCATTGCTG GGCCGCCTGT TCCTCCACGA
    CAAAGCACTT CTCAGCATAT CCCTAAACTC CCTCCAAAAA CTTACAAAAG GGAGCACACC
    CACCCGTCCA TGCACAGAGA CGGGCCACCA CTGTTGGAGA ATGCCCATTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212765.1
    CDS224..4180
    Translation

    Target ORF information:

    RefSeq Version XM_005212708.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212708.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA
    TCATCTATAA GTTTAACCAA GAGCACTGAT GAAGTGCCTG TCCCCCCTCC TGTTCCTCCA
    CGAAGACGGC CAGAGTCTGC CCCAGCGGAA TCTTCACCAT CTAAGATGAT GTCTAAGCAT
    TTGGACAGCC CCCCAGCAAT TCCTCCTAGG CAACCCACAT CAAAAGCCTA TTCACCACGG
    TATTCAATAT CAGACCGGAC CTCTATATCA GACCCTCCTG AAAGCCCTCC CTTATTACCT
    CCACGAGAAC CTGTGAGGAC ACCTGATGTT TTCTCAAGTT CACCACTACA TCTCCAACCT
    CCCCCTCTGG GCAAAAAAAG TGACCATAGT AATGCCTTCT TCCCAAACAG CCCCTCCCCC
    TTTACACCAC CTCCTCCTCA AACACCTTCT CCTCATGGAA CAAGAAGGCA TCTGCCGTCA
    CCACCACTGA CACAAGAAGT GGACCTCCAT TCCATTGCTG GGCCGCCTGT TCCTCCACGA
    CAAAGCACTT CTCAGCATAT CCCTAAACTC CCTCCAAAAA CTTACAAAAG GGAGCACACC
    CACCCGTCCA TGCACAGAGA CGGGCCACCA CTGTTGGAGA ATGCCCATTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06855
    Clone ID Related Accession (Same CDS sequence) XM_005212708.3 , XM_005193883.3 , XM_005212708.4
    Accession Version XM_005193883.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193883.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA
    TCATCTATAA GTTTAACCAA GAGCACTGAT GAAGTGCCTG TCCCCCCTCC TGTTCCTCCA
    CGAAGACGGC CAGAGTCTGC CCCAGCGGAA TCTTCACCAT CTAAGATGAT GTCTAAGCAT
    TTGGACAGCC CCCCAGCAAT TCCTCCTAGG CAACCCACAT CAAAAGCCTA TTCACCACGG
    TATTCAATAT CAGACCGGAC CTCTATATCA GACCCTCCTG AAAGCCCTCC CTTATTACCT
    CCACGAGAAC CTGTGAGGAC ACCTGATGTT TTCTCAAGTT CACCACTACA TCTCCAACCT
    CCCCCTCTGG GCAAAAAAAG TGACCATAGT AATGCCTTCT TCCCAAACAG CCCCTCCCCC
    TTTACACCAC CTCCTCCTCA AACACCTTCT CCTCATGGAA CAAGAAGGCA TCTGCCGTCA
    CCACCACTGA CACAAGAAGT GGACCTCCAT TCCATTGCTG GGCCGCCTGT TCCTCCACGA
    CAAAGCACTT CTCAGCATAT CCCTAAACTC CCTCCAAAAA CTTACAAAAG GGAGCACACC
    CACCCGTCCA TGCACAGAGA CGGGCCACCA CTGTTGGAGA ATGCCCATTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193940.1
    CDS224..4180
    Translation

    Target ORF information:

    RefSeq Version XM_005193883.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193883.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA
    TCATCTATAA GTTTAACCAA GAGCACTGAT GAAGTGCCTG TCCCCCCTCC TGTTCCTCCA
    CGAAGACGGC CAGAGTCTGC CCCAGCGGAA TCTTCACCAT CTAAGATGAT GTCTAAGCAT
    TTGGACAGCC CCCCAGCAAT TCCTCCTAGG CAACCCACAT CAAAAGCCTA TTCACCACGG
    TATTCAATAT CAGACCGGAC CTCTATATCA GACCCTCCTG AAAGCCCTCC CTTATTACCT
    CCACGAGAAC CTGTGAGGAC ACCTGATGTT TTCTCAAGTT CACCACTACA TCTCCAACCT
    CCCCCTCTGG GCAAAAAAAG TGACCATAGT AATGCCTTCT TCCCAAACAG CCCCTCCCCC
    TTTACACCAC CTCCTCCTCA AACACCTTCT CCTCATGGAA CAAGAAGGCA TCTGCCGTCA
    CCACCACTGA CACAAGAAGT GGACCTCCAT TCCATTGCTG GGCCGCCTGT TCCTCCACGA
    CAAAGCACTT CTCAGCATAT CCCTAAACTC CCTCCAAAAA CTTACAAAAG GGAGCACACC
    CACCCGTCCA TGCACAGAGA CGGGCCACCA CTGTTGGAGA ATGCCCATTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06855
    Clone ID Related Accession (Same CDS sequence) XM_005212708.3 , XM_005193883.3 , XM_005212708.4
    Accession Version XM_005212708.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212708.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA
    TCATCTATAA GTTTAACCAA GAGCACTGAT GAAGTGCCTG TCCCCCCTCC TGTTCCTCCA
    CGAAGACGGC CAGAGTCTGC CCCAGCGGAA TCTTCACCAT CTAAGATGAT GTCTAAGCAT
    TTGGACAGCC CCCCAGCAAT TCCTCCTAGG CAACCCACAT CAAAAGCCTA TTCACCACGG
    TATTCAATAT CAGACCGGAC CTCTATATCA GACCCTCCTG AAAGCCCTCC CTTATTACCT
    CCACGAGAAC CTGTGAGGAC ACCTGATGTT TTCTCAAGTT CACCACTACA TCTCCAACCT
    CCCCCTCTGG GCAAAAAAAG TGACCATAGT AATGCCTTCT TCCCAAACAG CCCCTCCCCC
    TTTACACCAC CTCCTCCTCA AACACCTTCT CCTCATGGAA CAAGAAGGCA TCTGCCGTCA
    CCACCACTGA CACAAGAAGT GGACCTCCAT TCCATTGCTG GGCCGCCTGT TCCTCCACGA
    CAAAGCACTT CTCAGCATAT CCCTAAACTC CCTCCAAAAA CTTACAAAAG GGAGCACACC
    CACCCGTCCA TGCACAGAGA CGGGCCACCA CTGTTGGAGA ATGCCCATTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212765.1
    CDS219..4175
    Translation

    Target ORF information:

    RefSeq Version XM_005212708.4
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212708.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGATC TGCTTCAGTA
    TCATCTATAA GTTTAACCAA GAGCACTGAT GAAGTGCCTG TCCCCCCTCC TGTTCCTCCA
    CGAAGACGGC CAGAGTCTGC CCCAGCGGAA TCTTCACCAT CTAAGATGAT GTCTAAGCAT
    TTGGACAGCC CCCCAGCAAT TCCTCCTAGG CAACCCACAT CAAAAGCCTA TTCACCACGG
    TATTCAATAT CAGACCGGAC CTCTATATCA GACCCTCCTG AAAGCCCTCC CTTATTACCT
    CCACGAGAAC CTGTGAGGAC ACCTGATGTT TTCTCAAGTT CACCACTACA TCTCCAACCT
    CCCCCTCTGG GCAAAAAAAG TGACCATAGT AATGCCTTCT TCCCAAACAG CCCCTCCCCC
    TTTACACCAC CTCCTCCTCA AACACCTTCT CCTCATGGAA CAAGAAGGCA TCTGCCGTCA
    CCACCACTGA CACAAGAAGT GGACCTCCAT TCCATTGCTG GGCCGCCTGT TCCTCCACGA
    CAAAGCACTT CTCAGCATAT CCCTAAACTC CCTCCAAAAA CTTACAAAAG GGAGCACACC
    CACCCGTCCA TGCACAGAGA CGGGCCACCA CTGTTGGAGA ATGCCCATTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06856
    Clone ID Related Accession (Same CDS sequence) XM_005193884.3 , XM_005212709.3 , XM_005212709.4
    Accession Version XM_005193884.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193884.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAGGTCC TAGGTTATAA AATTGACCAC
    CAGGTTTCTG TTTACATAGT AGCAGTATTA GAATACATTT CTGCAGACAT TTTAAAGCTG
    GTGGGGAATT ATGTGCGAAA TATACGGCAT TATGAAATTA CAAAACAAGA TATTAAAGTG
    GCAATGTGTG CTGATAAGGT ATTAATGGAT ATGTTTCATC AAGATGTGGA AGATATAAAT
    ATATTATCTT TAACTGATGA AGAGCCTTCC ACCTCAGGAG AGCAGACTTA CTATGATTTG
    GTAAAAGCAT TTATGGCAGA AATTCGACAA TACATAAGGG AACTTAATTT AATTATAAAA
    GTTTTTAGAG AGCCCTTTGT CTCCAATTCA AAATTGTTTT CAGCTAATGA TGTAGAAAAT
    ATATTTAGTC GTATAGTGGA TATACATGAA CTTAGTGTAA AATTACTGGG CCATATAGAA
    GATACTGTGG AAATGACAGA TGAAGGCAGT CCCCATCCAT TAGTAGGAAG CTGCTTTGAA
    GACTTAGCGG AGGAACTGGC ATTTGATCCA TATGAATCAT ATGCTCGAGA TATTCTACGA
    CCTGGTTTTC ACGATCGTTT CCTTAGTCAG TTATCAAAGC CTGGAGCAGC GCTCTATTTG
    CAGTCAATAG GCGAAGGTTT CAAAGAAGCT GTTCAATATG TTTTACCCAG ACTACTTCTA
    GCCCCTGTTT ACCACTGTCT GCATTACTTC GAACTTCTGA AGCAGTTAGA AGAAAAGAGT
    GAGGATCAAG AAGACAAAGA ATGTTTGAAA CAAGCAATAA CAGCTTTGCT TAATGTTCAG
    AGTGGTATGG AAAAAATATG TTCTAAAAGT CTTGCAAAAC GAAGACTTAG TGAATCTGCA
    TGTCGGTTTT ATAGTCAGCA AATGAAGGGG AAACAACTAG CAATCAAGAA AATGAACGAG
    ATTCAGAAGA ATATTGATGG TTGGGAGGGA AAAGACATTG GACAGTGTTG CAATGAGTTT
    ATAATGGAAG GAACTCTTAC ACGTGTAGGA GCCAAACATG AGAGACACAT ATTTCTCTTT
    GATGGCTTAA TGATTTGCTG TAAATCAAAT CATGGGCAGC CAAGACTACC TGGTGCTAGC
    AATGCAGAAT ATCGTCTTAA AGAAAAGTTT TTTATGAGAA AGGTACAAAT TAATGACAAA
    GATGACACCA GTGAGTACAA GCATGCTTTT GAAATAATTT TAAAAGATGA AAATAGTGTT
    ATATTTTCTG CCAAGTCAGC TGAAGAGAAG AACAATTGGA TGGCAGCATT GATATCTTTA
    CAGTACCGGA GTACCCTGGA AAGAATGCTT GATGTGACCA TGCTGCAGGA AGAAAAGGAG
    GAGCAGATGA GGCTCCCTAG TGCTGACGTT TATAGATTTG CAGAGCCTGA CTCTGAAGAA
    AATATCATAT TTGAAGAAAA CGTGCAGCCC AAAGCTGGAA TTCCAATTAT CAAGGCAGGA
    ACCGTTATTA AACTTATAGA GAGGCTCACA TACCACATGT ATGCAGATCC CAATTTTGTT
    CGGACATTTC TTACAACATA TAGATCCTTT TGTAAACCTC AAGAACTACT GAGTCTTATA
    ATAGAAAGGT TTGAAATTCC AGAGCCTGAG CCAACAGAAG CTGATCGCAT CGCTATAGAG
    AATGGGGATC AACCCTTGAG TGCAGAACTA AAGAGATTTA GAAAAGAATA TATACAGCCT
    GTGCAGCTGC GAGTATTAAA TGTATGTCGG CACTGGGTAG AGCACCACTT CTATGATTTT
    GAAAGAGATG CAGATCTTTT GCAGCGAATG GAGGAATTTA TTGGAACCGT AAGAGGTAAA
    GCAATGAAAA AATGGGTTGA ATCCATCACT AAAATAATCC AAAGGAAAAA AATTGCCAGA
    GACAATGGAC CAGGTCATAA TATTACATTT CAGAGTTCAC CTCCTACGGT TGAGTGGCAT
    ATAAGCAGAC CTGGGCACAT CGAGACTTTT GACCTGCTCA CCTTACACCC AATAGAAATT
    GCTCGACAAC TCACTTTACT TGAATCAGAT CTATATCGAG CTGTACAGCC ATCAGAATTA
    GTTGGAAGTG TGTGGACAAA AGAAGACAAA GAAATTAATT CTCCTAATCT TCTGAAAATG
    ATCCGGCACA CCACTAATCT CACTCTGTGG TTTGAGAAAT GTATTGTAGA AACTGAAAAC
    TTAGAAGAAA GAGTAGCTGT GGTGAGTCGA ATAATTGAGA TTCTACAAGT GTTCCAAGAG
    CTCAACAACT TCAATGGTGT TCTTGAGGTT GTCAGTGCTA TGAACTCATC CCCTGTTTAC
    AGACTAGACC ACACGTTTGA GCAAATACCA AGTCGCCAAA AGAAAATTTT AGAAGAAGCT
    CATGAACTAA GTGAAGATCA CTATAAGAAA TATTTGGCAA AACTCAGGTC TATTAATCCA
    CCATGTGTGC CTTTCTTTGG AATTTATCTC ACTAATATCT TGAAAACAGA AGAAGGCAAC
    CCTGAGGTCC TAAAACGGCA TGGAAAAGAG CTTATAAACT TTAGCAAAAG GAGGAAAGTA
    GCAGAAATAA CAGGCGAGAT CCAGCAGTAC CAAAATCAGC CTTACTGTTT ACGAGTAGAG
    TCAGATATCA AAAGGTTTTT TGAAAACTTG AATCCAATGG GAAATAGCAT GGAAAAAGAA
    TTTACGGATT ATCTTTTCAA CAAATCCCTA GAAATAGAAC CACGAAACCC TAAGCCTCTT
    CCAAGATTTC CAAAAAAATA CAACTATCCC CTAAAATCTC CTGGTGTTCG TCCATCAAAC
    CCAAGACCAG GTACTATGAG ACATCCCACA CCTCTGCAGC AAGAGCCAAG GAAAATTAGT
    TATAGTAGGA TCCCTGAAAG TGAAACAGAA AGTACAGCAT CTGCACCAAA TTCTCCAAGG
    ACACCGTTAA CACCTCCTCC TGCTTCTGGT GCTTCTAGTA CCACAGATGT TTGCAGCGTA
    TTTGATTCTG ATCATTCAAG CCCTTTTCAC TCAAGCAGCG ATACCGTCTT TATCCAAGTT
    ACACTGCCCC ATGGCCCAAG ATCTGCTTCA GTATCATCTA TAAGTTTAAC CAAGAGCACT
    GATGAAGTGC CTGTCCCCCC TCCTGTTCCT CCACGAAGAC GGCCAGAGTC TGCCCCAGCG
    GAATCTTCAC CATCTAAGAT GATGTCTAAG CATTTGGACA GCCCCCCAGC AATTCCTCCT
    AGGCAACCCA CATCAAAAGC CTATTCACCA CGGTATTCAA TATCAGACCG GACCTCTATA
    TCAGACCCTC CTGAAAGCCC TCCCTTATTA CCTCCACGAG AACCTGTGAG GACACCTGAT
    GTTTTCTCAA GTTCACCACT ACATCTCCAA CCTCCCCCTC TGGGCAAAAA AAGTGACCAT
    AGTAATGCCT TCTTCCCAAA CAGCCCCTCC CCCTTTACAC CACCTCCTCC TCAAACACCT
    TCTCCTCATG GAACAAGAAG GCATCTGCCG TCACCACCAC TGACACAAGA AGTGGACCTC
    CATTCCATTG CTGGGCCGCC TGTTCCTCCA CGACAAAGCA CTTCTCAGCA TATCCCTAAA
    CTCCCTCCAA AAACTTACAA AAGGGAGCAC ACCCACCCGT CCATGCACAG AGACGGGCCA
    CCACTGTTGG AGAATGCCCA TTCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193941.1
    CDS224..4093
    Translation

    Target ORF information:

    RefSeq Version XM_005193884.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193884.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAGGTCC TAGGTTATAA AATTGACCAC
    CAGGTTTCTG TTTACATAGT AGCAGTATTA GAATACATTT CTGCAGACAT TTTAAAGCTG
    GTGGGGAATT ATGTGCGAAA TATACGGCAT TATGAAATTA CAAAACAAGA TATTAAAGTG
    GCAATGTGTG CTGATAAGGT ATTAATGGAT ATGTTTCATC AAGATGTGGA AGATATAAAT
    ATATTATCTT TAACTGATGA AGAGCCTTCC ACCTCAGGAG AGCAGACTTA CTATGATTTG
    GTAAAAGCAT TTATGGCAGA AATTCGACAA TACATAAGGG AACTTAATTT AATTATAAAA
    GTTTTTAGAG AGCCCTTTGT CTCCAATTCA AAATTGTTTT CAGCTAATGA TGTAGAAAAT
    ATATTTAGTC GTATAGTGGA TATACATGAA CTTAGTGTAA AATTACTGGG CCATATAGAA
    GATACTGTGG AAATGACAGA TGAAGGCAGT CCCCATCCAT TAGTAGGAAG CTGCTTTGAA
    GACTTAGCGG AGGAACTGGC ATTTGATCCA TATGAATCAT ATGCTCGAGA TATTCTACGA
    CCTGGTTTTC ACGATCGTTT CCTTAGTCAG TTATCAAAGC CTGGAGCAGC GCTCTATTTG
    CAGTCAATAG GCGAAGGTTT CAAAGAAGCT GTTCAATATG TTTTACCCAG ACTACTTCTA
    GCCCCTGTTT ACCACTGTCT GCATTACTTC GAACTTCTGA AGCAGTTAGA AGAAAAGAGT
    GAGGATCAAG AAGACAAAGA ATGTTTGAAA CAAGCAATAA CAGCTTTGCT TAATGTTCAG
    AGTGGTATGG AAAAAATATG TTCTAAAAGT CTTGCAAAAC GAAGACTTAG TGAATCTGCA
    TGTCGGTTTT ATAGTCAGCA AATGAAGGGG AAACAACTAG CAATCAAGAA AATGAACGAG
    ATTCAGAAGA ATATTGATGG TTGGGAGGGA AAAGACATTG GACAGTGTTG CAATGAGTTT
    ATAATGGAAG GAACTCTTAC ACGTGTAGGA GCCAAACATG AGAGACACAT ATTTCTCTTT
    GATGGCTTAA TGATTTGCTG TAAATCAAAT CATGGGCAGC CAAGACTACC TGGTGCTAGC
    AATGCAGAAT ATCGTCTTAA AGAAAAGTTT TTTATGAGAA AGGTACAAAT TAATGACAAA
    GATGACACCA GTGAGTACAA GCATGCTTTT GAAATAATTT TAAAAGATGA AAATAGTGTT
    ATATTTTCTG CCAAGTCAGC TGAAGAGAAG AACAATTGGA TGGCAGCATT GATATCTTTA
    CAGTACCGGA GTACCCTGGA AAGAATGCTT GATGTGACCA TGCTGCAGGA AGAAAAGGAG
    GAGCAGATGA GGCTCCCTAG TGCTGACGTT TATAGATTTG CAGAGCCTGA CTCTGAAGAA
    AATATCATAT TTGAAGAAAA CGTGCAGCCC AAAGCTGGAA TTCCAATTAT CAAGGCAGGA
    ACCGTTATTA AACTTATAGA GAGGCTCACA TACCACATGT ATGCAGATCC CAATTTTGTT
    CGGACATTTC TTACAACATA TAGATCCTTT TGTAAACCTC AAGAACTACT GAGTCTTATA
    ATAGAAAGGT TTGAAATTCC AGAGCCTGAG CCAACAGAAG CTGATCGCAT CGCTATAGAG
    AATGGGGATC AACCCTTGAG TGCAGAACTA AAGAGATTTA GAAAAGAATA TATACAGCCT
    GTGCAGCTGC GAGTATTAAA TGTATGTCGG CACTGGGTAG AGCACCACTT CTATGATTTT
    GAAAGAGATG CAGATCTTTT GCAGCGAATG GAGGAATTTA TTGGAACCGT AAGAGGTAAA
    GCAATGAAAA AATGGGTTGA ATCCATCACT AAAATAATCC AAAGGAAAAA AATTGCCAGA
    GACAATGGAC CAGGTCATAA TATTACATTT CAGAGTTCAC CTCCTACGGT TGAGTGGCAT
    ATAAGCAGAC CTGGGCACAT CGAGACTTTT GACCTGCTCA CCTTACACCC AATAGAAATT
    GCTCGACAAC TCACTTTACT TGAATCAGAT CTATATCGAG CTGTACAGCC ATCAGAATTA
    GTTGGAAGTG TGTGGACAAA AGAAGACAAA GAAATTAATT CTCCTAATCT TCTGAAAATG
    ATCCGGCACA CCACTAATCT CACTCTGTGG TTTGAGAAAT GTATTGTAGA AACTGAAAAC
    TTAGAAGAAA GAGTAGCTGT GGTGAGTCGA ATAATTGAGA TTCTACAAGT GTTCCAAGAG
    CTCAACAACT TCAATGGTGT TCTTGAGGTT GTCAGTGCTA TGAACTCATC CCCTGTTTAC
    AGACTAGACC ACACGTTTGA GCAAATACCA AGTCGCCAAA AGAAAATTTT AGAAGAAGCT
    CATGAACTAA GTGAAGATCA CTATAAGAAA TATTTGGCAA AACTCAGGTC TATTAATCCA
    CCATGTGTGC CTTTCTTTGG AATTTATCTC ACTAATATCT TGAAAACAGA AGAAGGCAAC
    CCTGAGGTCC TAAAACGGCA TGGAAAAGAG CTTATAAACT TTAGCAAAAG GAGGAAAGTA
    GCAGAAATAA CAGGCGAGAT CCAGCAGTAC CAAAATCAGC CTTACTGTTT ACGAGTAGAG
    TCAGATATCA AAAGGTTTTT TGAAAACTTG AATCCAATGG GAAATAGCAT GGAAAAAGAA
    TTTACGGATT ATCTTTTCAA CAAATCCCTA GAAATAGAAC CACGAAACCC TAAGCCTCTT
    CCAAGATTTC CAAAAAAATA CAACTATCCC CTAAAATCTC CTGGTGTTCG TCCATCAAAC
    CCAAGACCAG GTACTATGAG ACATCCCACA CCTCTGCAGC AAGAGCCAAG GAAAATTAGT
    TATAGTAGGA TCCCTGAAAG TGAAACAGAA AGTACAGCAT CTGCACCAAA TTCTCCAAGG
    ACACCGTTAA CACCTCCTCC TGCTTCTGGT GCTTCTAGTA CCACAGATGT TTGCAGCGTA
    TTTGATTCTG ATCATTCAAG CCCTTTTCAC TCAAGCAGCG ATACCGTCTT TATCCAAGTT
    ACACTGCCCC ATGGCCCAAG ATCTGCTTCA GTATCATCTA TAAGTTTAAC CAAGAGCACT
    GATGAAGTGC CTGTCCCCCC TCCTGTTCCT CCACGAAGAC GGCCAGAGTC TGCCCCAGCG
    GAATCTTCAC CATCTAAGAT GATGTCTAAG CATTTGGACA GCCCCCCAGC AATTCCTCCT
    AGGCAACCCA CATCAAAAGC CTATTCACCA CGGTATTCAA TATCAGACCG GACCTCTATA
    TCAGACCCTC CTGAAAGCCC TCCCTTATTA CCTCCACGAG AACCTGTGAG GACACCTGAT
    GTTTTCTCAA GTTCACCACT ACATCTCCAA CCTCCCCCTC TGGGCAAAAA AAGTGACCAT
    AGTAATGCCT TCTTCCCAAA CAGCCCCTCC CCCTTTACAC CACCTCCTCC TCAAACACCT
    TCTCCTCATG GAACAAGAAG GCATCTGCCG TCACCACCAC TGACACAAGA AGTGGACCTC
    CATTCCATTG CTGGGCCGCC TGTTCCTCCA CGACAAAGCA CTTCTCAGCA TATCCCTAAA
    CTCCCTCCAA AAACTTACAA AAGGGAGCAC ACCCACCCGT CCATGCACAG AGACGGGCCA
    CCACTGTTGG AGAATGCCCA TTCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06856
    Clone ID Related Accession (Same CDS sequence) XM_005193884.3 , XM_005212709.3 , XM_005212709.4
    Accession Version XM_005212709.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212709.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAGGTCC TAGGTTATAA AATTGACCAC
    CAGGTTTCTG TTTACATAGT AGCAGTATTA GAATACATTT CTGCAGACAT TTTAAAGCTG
    GTGGGGAATT ATGTGCGAAA TATACGGCAT TATGAAATTA CAAAACAAGA TATTAAAGTG
    GCAATGTGTG CTGATAAGGT ATTAATGGAT ATGTTTCATC AAGATGTGGA AGATATAAAT
    ATATTATCTT TAACTGATGA AGAGCCTTCC ACCTCAGGAG AGCAGACTTA CTATGATTTG
    GTAAAAGCAT TTATGGCAGA AATTCGACAA TACATAAGGG AACTTAATTT AATTATAAAA
    GTTTTTAGAG AGCCCTTTGT CTCCAATTCA AAATTGTTTT CAGCTAATGA TGTAGAAAAT
    ATATTTAGTC GTATAGTGGA TATACATGAA CTTAGTGTAA AATTACTGGG CCATATAGAA
    GATACTGTGG AAATGACAGA TGAAGGCAGT CCCCATCCAT TAGTAGGAAG CTGCTTTGAA
    GACTTAGCGG AGGAACTGGC ATTTGATCCA TATGAATCAT ATGCTCGAGA TATTCTACGA
    CCTGGTTTTC ACGATCGTTT CCTTAGTCAG TTATCAAAGC CTGGAGCAGC GCTCTATTTG
    CAGTCAATAG GCGAAGGTTT CAAAGAAGCT GTTCAATATG TTTTACCCAG ACTACTTCTA
    GCCCCTGTTT ACCACTGTCT GCATTACTTC GAACTTCTGA AGCAGTTAGA AGAAAAGAGT
    GAGGATCAAG AAGACAAAGA ATGTTTGAAA CAAGCAATAA CAGCTTTGCT TAATGTTCAG
    AGTGGTATGG AAAAAATATG TTCTAAAAGT CTTGCAAAAC GAAGACTTAG TGAATCTGCA
    TGTCGGTTTT ATAGTCAGCA AATGAAGGGG AAACAACTAG CAATCAAGAA AATGAACGAG
    ATTCAGAAGA ATATTGATGG TTGGGAGGGA AAAGACATTG GACAGTGTTG CAATGAGTTT
    ATAATGGAAG GAACTCTTAC ACGTGTAGGA GCCAAACATG AGAGACACAT ATTTCTCTTT
    GATGGCTTAA TGATTTGCTG TAAATCAAAT CATGGGCAGC CAAGACTACC TGGTGCTAGC
    AATGCAGAAT ATCGTCTTAA AGAAAAGTTT TTTATGAGAA AGGTACAAAT TAATGACAAA
    GATGACACCA GTGAGTACAA GCATGCTTTT GAAATAATTT TAAAAGATGA AAATAGTGTT
    ATATTTTCTG CCAAGTCAGC TGAAGAGAAG AACAATTGGA TGGCAGCATT GATATCTTTA
    CAGTACCGGA GTACCCTGGA AAGAATGCTT GATGTGACCA TGCTGCAGGA AGAAAAGGAG
    GAGCAGATGA GGCTCCCTAG TGCTGACGTT TATAGATTTG CAGAGCCTGA CTCTGAAGAA
    AATATCATAT TTGAAGAAAA CGTGCAGCCC AAAGCTGGAA TTCCAATTAT CAAGGCAGGA
    ACCGTTATTA AACTTATAGA GAGGCTCACA TACCACATGT ATGCAGATCC CAATTTTGTT
    CGGACATTTC TTACAACATA TAGATCCTTT TGTAAACCTC AAGAACTACT GAGTCTTATA
    ATAGAAAGGT TTGAAATTCC AGAGCCTGAG CCAACAGAAG CTGATCGCAT CGCTATAGAG
    AATGGGGATC AACCCTTGAG TGCAGAACTA AAGAGATTTA GAAAAGAATA TATACAGCCT
    GTGCAGCTGC GAGTATTAAA TGTATGTCGG CACTGGGTAG AGCACCACTT CTATGATTTT
    GAAAGAGATG CAGATCTTTT GCAGCGAATG GAGGAATTTA TTGGAACCGT AAGAGGTAAA
    GCAATGAAAA AATGGGTTGA ATCCATCACT AAAATAATCC AAAGGAAAAA AATTGCCAGA
    GACAATGGAC CAGGTCATAA TATTACATTT CAGAGTTCAC CTCCTACGGT TGAGTGGCAT
    ATAAGCAGAC CTGGGCACAT CGAGACTTTT GACCTGCTCA CCTTACACCC AATAGAAATT
    GCTCGACAAC TCACTTTACT TGAATCAGAT CTATATCGAG CTGTACAGCC ATCAGAATTA
    GTTGGAAGTG TGTGGACAAA AGAAGACAAA GAAATTAATT CTCCTAATCT TCTGAAAATG
    ATCCGGCACA CCACTAATCT CACTCTGTGG TTTGAGAAAT GTATTGTAGA AACTGAAAAC
    TTAGAAGAAA GAGTAGCTGT GGTGAGTCGA ATAATTGAGA TTCTACAAGT GTTCCAAGAG
    CTCAACAACT TCAATGGTGT TCTTGAGGTT GTCAGTGCTA TGAACTCATC CCCTGTTTAC
    AGACTAGACC ACACGTTTGA GCAAATACCA AGTCGCCAAA AGAAAATTTT AGAAGAAGCT
    CATGAACTAA GTGAAGATCA CTATAAGAAA TATTTGGCAA AACTCAGGTC TATTAATCCA
    CCATGTGTGC CTTTCTTTGG AATTTATCTC ACTAATATCT TGAAAACAGA AGAAGGCAAC
    CCTGAGGTCC TAAAACGGCA TGGAAAAGAG CTTATAAACT TTAGCAAAAG GAGGAAAGTA
    GCAGAAATAA CAGGCGAGAT CCAGCAGTAC CAAAATCAGC CTTACTGTTT ACGAGTAGAG
    TCAGATATCA AAAGGTTTTT TGAAAACTTG AATCCAATGG GAAATAGCAT GGAAAAAGAA
    TTTACGGATT ATCTTTTCAA CAAATCCCTA GAAATAGAAC CACGAAACCC TAAGCCTCTT
    CCAAGATTTC CAAAAAAATA CAACTATCCC CTAAAATCTC CTGGTGTTCG TCCATCAAAC
    CCAAGACCAG GTACTATGAG ACATCCCACA CCTCTGCAGC AAGAGCCAAG GAAAATTAGT
    TATAGTAGGA TCCCTGAAAG TGAAACAGAA AGTACAGCAT CTGCACCAAA TTCTCCAAGG
    ACACCGTTAA CACCTCCTCC TGCTTCTGGT GCTTCTAGTA CCACAGATGT TTGCAGCGTA
    TTTGATTCTG ATCATTCAAG CCCTTTTCAC TCAAGCAGCG ATACCGTCTT TATCCAAGTT
    ACACTGCCCC ATGGCCCAAG ATCTGCTTCA GTATCATCTA TAAGTTTAAC CAAGAGCACT
    GATGAAGTGC CTGTCCCCCC TCCTGTTCCT CCACGAAGAC GGCCAGAGTC TGCCCCAGCG
    GAATCTTCAC CATCTAAGAT GATGTCTAAG CATTTGGACA GCCCCCCAGC AATTCCTCCT
    AGGCAACCCA CATCAAAAGC CTATTCACCA CGGTATTCAA TATCAGACCG GACCTCTATA
    TCAGACCCTC CTGAAAGCCC TCCCTTATTA CCTCCACGAG AACCTGTGAG GACACCTGAT
    GTTTTCTCAA GTTCACCACT ACATCTCCAA CCTCCCCCTC TGGGCAAAAA AAGTGACCAT
    AGTAATGCCT TCTTCCCAAA CAGCCCCTCC CCCTTTACAC CACCTCCTCC TCAAACACCT
    TCTCCTCATG GAACAAGAAG GCATCTGCCG TCACCACCAC TGACACAAGA AGTGGACCTC
    CATTCCATTG CTGGGCCGCC TGTTCCTCCA CGACAAAGCA CTTCTCAGCA TATCCCTAAA
    CTCCCTCCAA AAACTTACAA AAGGGAGCAC ACCCACCCGT CCATGCACAG AGACGGGCCA
    CCACTGTTGG AGAATGCCCA TTCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212766.1
    CDS224..4093
    Translation

    Target ORF information:

    RefSeq Version XM_005212709.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212709.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAGGTCC TAGGTTATAA AATTGACCAC
    CAGGTTTCTG TTTACATAGT AGCAGTATTA GAATACATTT CTGCAGACAT TTTAAAGCTG
    GTGGGGAATT ATGTGCGAAA TATACGGCAT TATGAAATTA CAAAACAAGA TATTAAAGTG
    GCAATGTGTG CTGATAAGGT ATTAATGGAT ATGTTTCATC AAGATGTGGA AGATATAAAT
    ATATTATCTT TAACTGATGA AGAGCCTTCC ACCTCAGGAG AGCAGACTTA CTATGATTTG
    GTAAAAGCAT TTATGGCAGA AATTCGACAA TACATAAGGG AACTTAATTT AATTATAAAA
    GTTTTTAGAG AGCCCTTTGT CTCCAATTCA AAATTGTTTT CAGCTAATGA TGTAGAAAAT
    ATATTTAGTC GTATAGTGGA TATACATGAA CTTAGTGTAA AATTACTGGG CCATATAGAA
    GATACTGTGG AAATGACAGA TGAAGGCAGT CCCCATCCAT TAGTAGGAAG CTGCTTTGAA
    GACTTAGCGG AGGAACTGGC ATTTGATCCA TATGAATCAT ATGCTCGAGA TATTCTACGA
    CCTGGTTTTC ACGATCGTTT CCTTAGTCAG TTATCAAAGC CTGGAGCAGC GCTCTATTTG
    CAGTCAATAG GCGAAGGTTT CAAAGAAGCT GTTCAATATG TTTTACCCAG ACTACTTCTA
    GCCCCTGTTT ACCACTGTCT GCATTACTTC GAACTTCTGA AGCAGTTAGA AGAAAAGAGT
    GAGGATCAAG AAGACAAAGA ATGTTTGAAA CAAGCAATAA CAGCTTTGCT TAATGTTCAG
    AGTGGTATGG AAAAAATATG TTCTAAAAGT CTTGCAAAAC GAAGACTTAG TGAATCTGCA
    TGTCGGTTTT ATAGTCAGCA AATGAAGGGG AAACAACTAG CAATCAAGAA AATGAACGAG
    ATTCAGAAGA ATATTGATGG TTGGGAGGGA AAAGACATTG GACAGTGTTG CAATGAGTTT
    ATAATGGAAG GAACTCTTAC ACGTGTAGGA GCCAAACATG AGAGACACAT ATTTCTCTTT
    GATGGCTTAA TGATTTGCTG TAAATCAAAT CATGGGCAGC CAAGACTACC TGGTGCTAGC
    AATGCAGAAT ATCGTCTTAA AGAAAAGTTT TTTATGAGAA AGGTACAAAT TAATGACAAA
    GATGACACCA GTGAGTACAA GCATGCTTTT GAAATAATTT TAAAAGATGA AAATAGTGTT
    ATATTTTCTG CCAAGTCAGC TGAAGAGAAG AACAATTGGA TGGCAGCATT GATATCTTTA
    CAGTACCGGA GTACCCTGGA AAGAATGCTT GATGTGACCA TGCTGCAGGA AGAAAAGGAG
    GAGCAGATGA GGCTCCCTAG TGCTGACGTT TATAGATTTG CAGAGCCTGA CTCTGAAGAA
    AATATCATAT TTGAAGAAAA CGTGCAGCCC AAAGCTGGAA TTCCAATTAT CAAGGCAGGA
    ACCGTTATTA AACTTATAGA GAGGCTCACA TACCACATGT ATGCAGATCC CAATTTTGTT
    CGGACATTTC TTACAACATA TAGATCCTTT TGTAAACCTC AAGAACTACT GAGTCTTATA
    ATAGAAAGGT TTGAAATTCC AGAGCCTGAG CCAACAGAAG CTGATCGCAT CGCTATAGAG
    AATGGGGATC AACCCTTGAG TGCAGAACTA AAGAGATTTA GAAAAGAATA TATACAGCCT
    GTGCAGCTGC GAGTATTAAA TGTATGTCGG CACTGGGTAG AGCACCACTT CTATGATTTT
    GAAAGAGATG CAGATCTTTT GCAGCGAATG GAGGAATTTA TTGGAACCGT AAGAGGTAAA
    GCAATGAAAA AATGGGTTGA ATCCATCACT AAAATAATCC AAAGGAAAAA AATTGCCAGA
    GACAATGGAC CAGGTCATAA TATTACATTT CAGAGTTCAC CTCCTACGGT TGAGTGGCAT
    ATAAGCAGAC CTGGGCACAT CGAGACTTTT GACCTGCTCA CCTTACACCC AATAGAAATT
    GCTCGACAAC TCACTTTACT TGAATCAGAT CTATATCGAG CTGTACAGCC ATCAGAATTA
    GTTGGAAGTG TGTGGACAAA AGAAGACAAA GAAATTAATT CTCCTAATCT TCTGAAAATG
    ATCCGGCACA CCACTAATCT CACTCTGTGG TTTGAGAAAT GTATTGTAGA AACTGAAAAC
    TTAGAAGAAA GAGTAGCTGT GGTGAGTCGA ATAATTGAGA TTCTACAAGT GTTCCAAGAG
    CTCAACAACT TCAATGGTGT TCTTGAGGTT GTCAGTGCTA TGAACTCATC CCCTGTTTAC
    AGACTAGACC ACACGTTTGA GCAAATACCA AGTCGCCAAA AGAAAATTTT AGAAGAAGCT
    CATGAACTAA GTGAAGATCA CTATAAGAAA TATTTGGCAA AACTCAGGTC TATTAATCCA
    CCATGTGTGC CTTTCTTTGG AATTTATCTC ACTAATATCT TGAAAACAGA AGAAGGCAAC
    CCTGAGGTCC TAAAACGGCA TGGAAAAGAG CTTATAAACT TTAGCAAAAG GAGGAAAGTA
    GCAGAAATAA CAGGCGAGAT CCAGCAGTAC CAAAATCAGC CTTACTGTTT ACGAGTAGAG
    TCAGATATCA AAAGGTTTTT TGAAAACTTG AATCCAATGG GAAATAGCAT GGAAAAAGAA
    TTTACGGATT ATCTTTTCAA CAAATCCCTA GAAATAGAAC CACGAAACCC TAAGCCTCTT
    CCAAGATTTC CAAAAAAATA CAACTATCCC CTAAAATCTC CTGGTGTTCG TCCATCAAAC
    CCAAGACCAG GTACTATGAG ACATCCCACA CCTCTGCAGC AAGAGCCAAG GAAAATTAGT
    TATAGTAGGA TCCCTGAAAG TGAAACAGAA AGTACAGCAT CTGCACCAAA TTCTCCAAGG
    ACACCGTTAA CACCTCCTCC TGCTTCTGGT GCTTCTAGTA CCACAGATGT TTGCAGCGTA
    TTTGATTCTG ATCATTCAAG CCCTTTTCAC TCAAGCAGCG ATACCGTCTT TATCCAAGTT
    ACACTGCCCC ATGGCCCAAG ATCTGCTTCA GTATCATCTA TAAGTTTAAC CAAGAGCACT
    GATGAAGTGC CTGTCCCCCC TCCTGTTCCT CCACGAAGAC GGCCAGAGTC TGCCCCAGCG
    GAATCTTCAC CATCTAAGAT GATGTCTAAG CATTTGGACA GCCCCCCAGC AATTCCTCCT
    AGGCAACCCA CATCAAAAGC CTATTCACCA CGGTATTCAA TATCAGACCG GACCTCTATA
    TCAGACCCTC CTGAAAGCCC TCCCTTATTA CCTCCACGAG AACCTGTGAG GACACCTGAT
    GTTTTCTCAA GTTCACCACT ACATCTCCAA CCTCCCCCTC TGGGCAAAAA AAGTGACCAT
    AGTAATGCCT TCTTCCCAAA CAGCCCCTCC CCCTTTACAC CACCTCCTCC TCAAACACCT
    TCTCCTCATG GAACAAGAAG GCATCTGCCG TCACCACCAC TGACACAAGA AGTGGACCTC
    CATTCCATTG CTGGGCCGCC TGTTCCTCCA CGACAAAGCA CTTCTCAGCA TATCCCTAAA
    CTCCCTCCAA AAACTTACAA AAGGGAGCAC ACCCACCCGT CCATGCACAG AGACGGGCCA
    CCACTGTTGG AGAATGCCCA TTCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06856
    Clone ID Related Accession (Same CDS sequence) XM_005193884.3 , XM_005212709.3 , XM_005212709.4
    Accession Version XM_005212709.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212709.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAGGTCC TAGGTTATAA AATTGACCAC
    CAGGTTTCTG TTTACATAGT AGCAGTATTA GAATACATTT CTGCAGACAT TTTAAAGCTG
    GTGGGGAATT ATGTGCGAAA TATACGGCAT TATGAAATTA CAAAACAAGA TATTAAAGTG
    GCAATGTGTG CTGATAAGGT ATTAATGGAT ATGTTTCATC AAGATGTGGA AGATATAAAT
    ATATTATCTT TAACTGATGA AGAGCCTTCC ACCTCAGGAG AGCAGACTTA CTATGATTTG
    GTAAAAGCAT TTATGGCAGA AATTCGACAA TACATAAGGG AACTTAATTT AATTATAAAA
    GTTTTTAGAG AGCCCTTTGT CTCCAATTCA AAATTGTTTT CAGCTAATGA TGTAGAAAAT
    ATATTTAGTC GTATAGTGGA TATACATGAA CTTAGTGTAA AATTACTGGG CCATATAGAA
    GATACTGTGG AAATGACAGA TGAAGGCAGT CCCCATCCAT TAGTAGGAAG CTGCTTTGAA
    GACTTAGCGG AGGAACTGGC ATTTGATCCA TATGAATCAT ATGCTCGAGA TATTCTACGA
    CCTGGTTTTC ACGATCGTTT CCTTAGTCAG TTATCAAAGC CTGGAGCAGC GCTCTATTTG
    CAGTCAATAG GCGAAGGTTT CAAAGAAGCT GTTCAATATG TTTTACCCAG ACTACTTCTA
    GCCCCTGTTT ACCACTGTCT GCATTACTTC GAACTTCTGA AGCAGTTAGA AGAAAAGAGT
    GAGGATCAAG AAGACAAAGA ATGTTTGAAA CAAGCAATAA CAGCTTTGCT TAATGTTCAG
    AGTGGTATGG AAAAAATATG TTCTAAAAGT CTTGCAAAAC GAAGACTTAG TGAATCTGCA
    TGTCGGTTTT ATAGTCAGCA AATGAAGGGG AAACAACTAG CAATCAAGAA AATGAACGAG
    ATTCAGAAGA ATATTGATGG TTGGGAGGGA AAAGACATTG GACAGTGTTG CAATGAGTTT
    ATAATGGAAG GAACTCTTAC ACGTGTAGGA GCCAAACATG AGAGACACAT ATTTCTCTTT
    GATGGCTTAA TGATTTGCTG TAAATCAAAT CATGGGCAGC CAAGACTACC TGGTGCTAGC
    AATGCAGAAT ATCGTCTTAA AGAAAAGTTT TTTATGAGAA AGGTACAAAT TAATGACAAA
    GATGACACCA GTGAGTACAA GCATGCTTTT GAAATAATTT TAAAAGATGA AAATAGTGTT
    ATATTTTCTG CCAAGTCAGC TGAAGAGAAG AACAATTGGA TGGCAGCATT GATATCTTTA
    CAGTACCGGA GTACCCTGGA AAGAATGCTT GATGTGACCA TGCTGCAGGA AGAAAAGGAG
    GAGCAGATGA GGCTCCCTAG TGCTGACGTT TATAGATTTG CAGAGCCTGA CTCTGAAGAA
    AATATCATAT TTGAAGAAAA CGTGCAGCCC AAAGCTGGAA TTCCAATTAT CAAGGCAGGA
    ACCGTTATTA AACTTATAGA GAGGCTCACA TACCACATGT ATGCAGATCC CAATTTTGTT
    CGGACATTTC TTACAACATA TAGATCCTTT TGTAAACCTC AAGAACTACT GAGTCTTATA
    ATAGAAAGGT TTGAAATTCC AGAGCCTGAG CCAACAGAAG CTGATCGCAT CGCTATAGAG
    AATGGGGATC AACCCTTGAG TGCAGAACTA AAGAGATTTA GAAAAGAATA TATACAGCCT
    GTGCAGCTGC GAGTATTAAA TGTATGTCGG CACTGGGTAG AGCACCACTT CTATGATTTT
    GAAAGAGATG CAGATCTTTT GCAGCGAATG GAGGAATTTA TTGGAACCGT AAGAGGTAAA
    GCAATGAAAA AATGGGTTGA ATCCATCACT AAAATAATCC AAAGGAAAAA AATTGCCAGA
    GACAATGGAC CAGGTCATAA TATTACATTT CAGAGTTCAC CTCCTACGGT TGAGTGGCAT
    ATAAGCAGAC CTGGGCACAT CGAGACTTTT GACCTGCTCA CCTTACACCC AATAGAAATT
    GCTCGACAAC TCACTTTACT TGAATCAGAT CTATATCGAG CTGTACAGCC ATCAGAATTA
    GTTGGAAGTG TGTGGACAAA AGAAGACAAA GAAATTAATT CTCCTAATCT TCTGAAAATG
    ATCCGGCACA CCACTAATCT CACTCTGTGG TTTGAGAAAT GTATTGTAGA AACTGAAAAC
    TTAGAAGAAA GAGTAGCTGT GGTGAGTCGA ATAATTGAGA TTCTACAAGT GTTCCAAGAG
    CTCAACAACT TCAATGGTGT TCTTGAGGTT GTCAGTGCTA TGAACTCATC CCCTGTTTAC
    AGACTAGACC ACACGTTTGA GCAAATACCA AGTCGCCAAA AGAAAATTTT AGAAGAAGCT
    CATGAACTAA GTGAAGATCA CTATAAGAAA TATTTGGCAA AACTCAGGTC TATTAATCCA
    CCATGTGTGC CTTTCTTTGG AATTTATCTC ACTAATATCT TGAAAACAGA AGAAGGCAAC
    CCTGAGGTCC TAAAACGGCA TGGAAAAGAG CTTATAAACT TTAGCAAAAG GAGGAAAGTA
    GCAGAAATAA CAGGCGAGAT CCAGCAGTAC CAAAATCAGC CTTACTGTTT ACGAGTAGAG
    TCAGATATCA AAAGGTTTTT TGAAAACTTG AATCCAATGG GAAATAGCAT GGAAAAAGAA
    TTTACGGATT ATCTTTTCAA CAAATCCCTA GAAATAGAAC CACGAAACCC TAAGCCTCTT
    CCAAGATTTC CAAAAAAATA CAACTATCCC CTAAAATCTC CTGGTGTTCG TCCATCAAAC
    CCAAGACCAG GTACTATGAG ACATCCCACA CCTCTGCAGC AAGAGCCAAG GAAAATTAGT
    TATAGTAGGA TCCCTGAAAG TGAAACAGAA AGTACAGCAT CTGCACCAAA TTCTCCAAGG
    ACACCGTTAA CACCTCCTCC TGCTTCTGGT GCTTCTAGTA CCACAGATGT TTGCAGCGTA
    TTTGATTCTG ATCATTCAAG CCCTTTTCAC TCAAGCAGCG ATACCGTCTT TATCCAAGTT
    ACACTGCCCC ATGGCCCAAG ATCTGCTTCA GTATCATCTA TAAGTTTAAC CAAGAGCACT
    GATGAAGTGC CTGTCCCCCC TCCTGTTCCT CCACGAAGAC GGCCAGAGTC TGCCCCAGCG
    GAATCTTCAC CATCTAAGAT GATGTCTAAG CATTTGGACA GCCCCCCAGC AATTCCTCCT
    AGGCAACCCA CATCAAAAGC CTATTCACCA CGGTATTCAA TATCAGACCG GACCTCTATA
    TCAGACCCTC CTGAAAGCCC TCCCTTATTA CCTCCACGAG AACCTGTGAG GACACCTGAT
    GTTTTCTCAA GTTCACCACT ACATCTCCAA CCTCCCCCTC TGGGCAAAAA AAGTGACCAT
    AGTAATGCCT TCTTCCCAAA CAGCCCCTCC CCCTTTACAC CACCTCCTCC TCAAACACCT
    TCTCCTCATG GAACAAGAAG GCATCTGCCG TCACCACCAC TGACACAAGA AGTGGACCTC
    CATTCCATTG CTGGGCCGCC TGTTCCTCCA CGACAAAGCA CTTCTCAGCA TATCCCTAAA
    CTCCCTCCAA AAACTTACAA AAGGGAGCAC ACCCACCCGT CCATGCACAG AGACGGGCCA
    CCACTGTTGG AGAATGCCCA TTCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212766.1
    CDS219..4088
    Translation

    Target ORF information:

    RefSeq Version XM_005212709.4
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212709.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAGGTCC TAGGTTATAA AATTGACCAC
    CAGGTTTCTG TTTACATAGT AGCAGTATTA GAATACATTT CTGCAGACAT TTTAAAGCTG
    GTGGGGAATT ATGTGCGAAA TATACGGCAT TATGAAATTA CAAAACAAGA TATTAAAGTG
    GCAATGTGTG CTGATAAGGT ATTAATGGAT ATGTTTCATC AAGATGTGGA AGATATAAAT
    ATATTATCTT TAACTGATGA AGAGCCTTCC ACCTCAGGAG AGCAGACTTA CTATGATTTG
    GTAAAAGCAT TTATGGCAGA AATTCGACAA TACATAAGGG AACTTAATTT AATTATAAAA
    GTTTTTAGAG AGCCCTTTGT CTCCAATTCA AAATTGTTTT CAGCTAATGA TGTAGAAAAT
    ATATTTAGTC GTATAGTGGA TATACATGAA CTTAGTGTAA AATTACTGGG CCATATAGAA
    GATACTGTGG AAATGACAGA TGAAGGCAGT CCCCATCCAT TAGTAGGAAG CTGCTTTGAA
    GACTTAGCGG AGGAACTGGC ATTTGATCCA TATGAATCAT ATGCTCGAGA TATTCTACGA
    CCTGGTTTTC ACGATCGTTT CCTTAGTCAG TTATCAAAGC CTGGAGCAGC GCTCTATTTG
    CAGTCAATAG GCGAAGGTTT CAAAGAAGCT GTTCAATATG TTTTACCCAG ACTACTTCTA
    GCCCCTGTTT ACCACTGTCT GCATTACTTC GAACTTCTGA AGCAGTTAGA AGAAAAGAGT
    GAGGATCAAG AAGACAAAGA ATGTTTGAAA CAAGCAATAA CAGCTTTGCT TAATGTTCAG
    AGTGGTATGG AAAAAATATG TTCTAAAAGT CTTGCAAAAC GAAGACTTAG TGAATCTGCA
    TGTCGGTTTT ATAGTCAGCA AATGAAGGGG AAACAACTAG CAATCAAGAA AATGAACGAG
    ATTCAGAAGA ATATTGATGG TTGGGAGGGA AAAGACATTG GACAGTGTTG CAATGAGTTT
    ATAATGGAAG GAACTCTTAC ACGTGTAGGA GCCAAACATG AGAGACACAT ATTTCTCTTT
    GATGGCTTAA TGATTTGCTG TAAATCAAAT CATGGGCAGC CAAGACTACC TGGTGCTAGC
    AATGCAGAAT ATCGTCTTAA AGAAAAGTTT TTTATGAGAA AGGTACAAAT TAATGACAAA
    GATGACACCA GTGAGTACAA GCATGCTTTT GAAATAATTT TAAAAGATGA AAATAGTGTT
    ATATTTTCTG CCAAGTCAGC TGAAGAGAAG AACAATTGGA TGGCAGCATT GATATCTTTA
    CAGTACCGGA GTACCCTGGA AAGAATGCTT GATGTGACCA TGCTGCAGGA AGAAAAGGAG
    GAGCAGATGA GGCTCCCTAG TGCTGACGTT TATAGATTTG CAGAGCCTGA CTCTGAAGAA
    AATATCATAT TTGAAGAAAA CGTGCAGCCC AAAGCTGGAA TTCCAATTAT CAAGGCAGGA
    ACCGTTATTA AACTTATAGA GAGGCTCACA TACCACATGT ATGCAGATCC CAATTTTGTT
    CGGACATTTC TTACAACATA TAGATCCTTT TGTAAACCTC AAGAACTACT GAGTCTTATA
    ATAGAAAGGT TTGAAATTCC AGAGCCTGAG CCAACAGAAG CTGATCGCAT CGCTATAGAG
    AATGGGGATC AACCCTTGAG TGCAGAACTA AAGAGATTTA GAAAAGAATA TATACAGCCT
    GTGCAGCTGC GAGTATTAAA TGTATGTCGG CACTGGGTAG AGCACCACTT CTATGATTTT
    GAAAGAGATG CAGATCTTTT GCAGCGAATG GAGGAATTTA TTGGAACCGT AAGAGGTAAA
    GCAATGAAAA AATGGGTTGA ATCCATCACT AAAATAATCC AAAGGAAAAA AATTGCCAGA
    GACAATGGAC CAGGTCATAA TATTACATTT CAGAGTTCAC CTCCTACGGT TGAGTGGCAT
    ATAAGCAGAC CTGGGCACAT CGAGACTTTT GACCTGCTCA CCTTACACCC AATAGAAATT
    GCTCGACAAC TCACTTTACT TGAATCAGAT CTATATCGAG CTGTACAGCC ATCAGAATTA
    GTTGGAAGTG TGTGGACAAA AGAAGACAAA GAAATTAATT CTCCTAATCT TCTGAAAATG
    ATCCGGCACA CCACTAATCT CACTCTGTGG TTTGAGAAAT GTATTGTAGA AACTGAAAAC
    TTAGAAGAAA GAGTAGCTGT GGTGAGTCGA ATAATTGAGA TTCTACAAGT GTTCCAAGAG
    CTCAACAACT TCAATGGTGT TCTTGAGGTT GTCAGTGCTA TGAACTCATC CCCTGTTTAC
    AGACTAGACC ACACGTTTGA GCAAATACCA AGTCGCCAAA AGAAAATTTT AGAAGAAGCT
    CATGAACTAA GTGAAGATCA CTATAAGAAA TATTTGGCAA AACTCAGGTC TATTAATCCA
    CCATGTGTGC CTTTCTTTGG AATTTATCTC ACTAATATCT TGAAAACAGA AGAAGGCAAC
    CCTGAGGTCC TAAAACGGCA TGGAAAAGAG CTTATAAACT TTAGCAAAAG GAGGAAAGTA
    GCAGAAATAA CAGGCGAGAT CCAGCAGTAC CAAAATCAGC CTTACTGTTT ACGAGTAGAG
    TCAGATATCA AAAGGTTTTT TGAAAACTTG AATCCAATGG GAAATAGCAT GGAAAAAGAA
    TTTACGGATT ATCTTTTCAA CAAATCCCTA GAAATAGAAC CACGAAACCC TAAGCCTCTT
    CCAAGATTTC CAAAAAAATA CAACTATCCC CTAAAATCTC CTGGTGTTCG TCCATCAAAC
    CCAAGACCAG GTACTATGAG ACATCCCACA CCTCTGCAGC AAGAGCCAAG GAAAATTAGT
    TATAGTAGGA TCCCTGAAAG TGAAACAGAA AGTACAGCAT CTGCACCAAA TTCTCCAAGG
    ACACCGTTAA CACCTCCTCC TGCTTCTGGT GCTTCTAGTA CCACAGATGT TTGCAGCGTA
    TTTGATTCTG ATCATTCAAG CCCTTTTCAC TCAAGCAGCG ATACCGTCTT TATCCAAGTT
    ACACTGCCCC ATGGCCCAAG ATCTGCTTCA GTATCATCTA TAAGTTTAAC CAAGAGCACT
    GATGAAGTGC CTGTCCCCCC TCCTGTTCCT CCACGAAGAC GGCCAGAGTC TGCCCCAGCG
    GAATCTTCAC CATCTAAGAT GATGTCTAAG CATTTGGACA GCCCCCCAGC AATTCCTCCT
    AGGCAACCCA CATCAAAAGC CTATTCACCA CGGTATTCAA TATCAGACCG GACCTCTATA
    TCAGACCCTC CTGAAAGCCC TCCCTTATTA CCTCCACGAG AACCTGTGAG GACACCTGAT
    GTTTTCTCAA GTTCACCACT ACATCTCCAA CCTCCCCCTC TGGGCAAAAA AAGTGACCAT
    AGTAATGCCT TCTTCCCAAA CAGCCCCTCC CCCTTTACAC CACCTCCTCC TCAAACACCT
    TCTCCTCATG GAACAAGAAG GCATCTGCCG TCACCACCAC TGACACAAGA AGTGGACCTC
    CATTCCATTG CTGGGCCGCC TGTTCCTCCA CGACAAAGCA CTTCTCAGCA TATCCCTAAA
    CTCCCTCCAA AAACTTACAA AAGGGAGCAC ACCCACCCGT CCATGCACAG AGACGGGCCA
    CCACTGTTGG AGAATGCCCA TTCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06854
    Clone ID Related Accession (Same CDS sequence) XM_002691241.6 , XM_617859.8 , XM_002691241.5
    Accession Version XM_002691241.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691241.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCGTC
    TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCATC TATAAGTTTA
    ACCAAGAGCA CTGATGAAGT GCCTGTCCCC CCTCCTGTTC CTCCACGAAG ACGGCCAGAG
    TCTGCCCCAG CGGAATCTTC ACCATCTAAG ATGATGTCTA AGCATTTGGA CAGCCCCCCA
    GCAATTCCTC CTAGGCAACC CACATCAAAA GCCTATTCAC CACGGTATTC AATATCAGAC
    CGGACCTCTA TATCAGACCC TCCTGAAAGC CCTCCCTTAT TACCTCCACG AGAACCTGTG
    AGGACACCTG ATGTTTTCTC AAGTTCACCA CTACATCTCC AACCTCCCCC TCTGGGCAAA
    AAAAGTGACC ATAGTAATGC CTTCTTCCCA AACAGCCCCT CCCCCTTTAC ACCACCTCCT
    CCTCAAACAC CTTCTCCTCA TGGAACAAGA AGGCATCTGC CGTCACCACC ACTGACACAA
    GAAGTGGACC TCCATTCCAT TGCTGGGCCG CCTGTTCCTC CACGACAAAG CACTTCTCAG
    CATATCCCTA AACTCCCTCC AAAAACTTAC AAAAGGGAGC ACACCCACCC GTCCATGCAC
    AGAGACGGGC CACCACTGTT GGAGAATGCC CATTCTTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691287.2
    CDS219..4220
    Translation

    Target ORF information:

    RefSeq Version XM_002691241.6
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691241.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCGTC
    TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCATC TATAAGTTTA
    ACCAAGAGCA CTGATGAAGT GCCTGTCCCC CCTCCTGTTC CTCCACGAAG ACGGCCAGAG
    TCTGCCCCAG CGGAATCTTC ACCATCTAAG ATGATGTCTA AGCATTTGGA CAGCCCCCCA
    GCAATTCCTC CTAGGCAACC CACATCAAAA GCCTATTCAC CACGGTATTC AATATCAGAC
    CGGACCTCTA TATCAGACCC TCCTGAAAGC CCTCCCTTAT TACCTCCACG AGAACCTGTG
    AGGACACCTG ATGTTTTCTC AAGTTCACCA CTACATCTCC AACCTCCCCC TCTGGGCAAA
    AAAAGTGACC ATAGTAATGC CTTCTTCCCA AACAGCCCCT CCCCCTTTAC ACCACCTCCT
    CCTCAAACAC CTTCTCCTCA TGGAACAAGA AGGCATCTGC CGTCACCACC ACTGACACAA
    GAAGTGGACC TCCATTCCAT TGCTGGGCCG CCTGTTCCTC CACGACAAAG CACTTCTCAG
    CATATCCCTA AACTCCCTCC AAAAACTTAC AAAAGGGAGC ACACCCACCC GTCCATGCAC
    AGAGACGGGC CACCACTGTT GGAGAATGCC CATTCTTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06854
    Clone ID Related Accession (Same CDS sequence) XM_002691241.6 , XM_617859.8 , XM_002691241.5
    Accession Version XM_617859.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_617859.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCGTC
    TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCATC TATAAGTTTA
    ACCAAGAGCA CTGATGAAGT GCCTGTCCCC CCTCCTGTTC CTCCACGAAG ACGGCCAGAG
    TCTGCCCCAG CGGAATCTTC ACCATCTAAG ATGATGTCTA AGCATTTGGA CAGCCCCCCA
    GCAATTCCTC CTAGGCAACC CACATCAAAA GCCTATTCAC CACGGTATTC AATATCAGAC
    CGGACCTCTA TATCAGACCC TCCTGAAAGC CCTCCCTTAT TACCTCCACG AGAACCTGTG
    AGGACACCTG ATGTTTTCTC AAGTTCACCA CTACATCTCC AACCTCCCCC TCTGGGCAAA
    AAAAGTGACC ATAGTAATGC CTTCTTCCCA AACAGCCCCT CCCCCTTTAC ACCACCTCCT
    CCTCAAACAC CTTCTCCTCA TGGAACAAGA AGGCATCTGC CGTCACCACC ACTGACACAA
    GAAGTGGACC TCCATTCCAT TGCTGGGCCG CCTGTTCCTC CACGACAAAG CACTTCTCAG
    CATATCCCTA AACTCCCTCC AAAAACTTAC AAAAGGGAGC ACACCCACCC GTCCATGCAC
    AGAGACGGGC CACCACTGTT GGAGAATGCC CATTCTTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_617859.5
    CDS225..4226
    Translation

    Target ORF information:

    RefSeq Version XM_617859.8
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_617859.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCGTC
    TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCATC TATAAGTTTA
    ACCAAGAGCA CTGATGAAGT GCCTGTCCCC CCTCCTGTTC CTCCACGAAG ACGGCCAGAG
    TCTGCCCCAG CGGAATCTTC ACCATCTAAG ATGATGTCTA AGCATTTGGA CAGCCCCCCA
    GCAATTCCTC CTAGGCAACC CACATCAAAA GCCTATTCAC CACGGTATTC AATATCAGAC
    CGGACCTCTA TATCAGACCC TCCTGAAAGC CCTCCCTTAT TACCTCCACG AGAACCTGTG
    AGGACACCTG ATGTTTTCTC AAGTTCACCA CTACATCTCC AACCTCCCCC TCTGGGCAAA
    AAAAGTGACC ATAGTAATGC CTTCTTCCCA AACAGCCCCT CCCCCTTTAC ACCACCTCCT
    CCTCAAACAC CTTCTCCTCA TGGAACAAGA AGGCATCTGC CGTCACCACC ACTGACACAA
    GAAGTGGACC TCCATTCCAT TGCTGGGCCG CCTGTTCCTC CACGACAAAG CACTTCTCAG
    CATATCCCTA AACTCCCTCC AAAAACTTAC AAAAGGGAGC ACACCCACCC GTCCATGCAC
    AGAGACGGGC CACCACTGTT GGAGAATGCC CATTCTTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06854
    Clone ID Related Accession (Same CDS sequence) XM_002691241.6 , XM_617859.8 , XM_002691241.5
    Accession Version XM_002691241.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product son of sevenless homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691241.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCGTC
    TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCATC TATAAGTTTA
    ACCAAGAGCA CTGATGAAGT GCCTGTCCCC CCTCCTGTTC CTCCACGAAG ACGGCCAGAG
    TCTGCCCCAG CGGAATCTTC ACCATCTAAG ATGATGTCTA AGCATTTGGA CAGCCCCCCA
    GCAATTCCTC CTAGGCAACC CACATCAAAA GCCTATTCAC CACGGTATTC AATATCAGAC
    CGGACCTCTA TATCAGACCC TCCTGAAAGC CCTCCCTTAT TACCTCCACG AGAACCTGTG
    AGGACACCTG ATGTTTTCTC AAGTTCACCA CTACATCTCC AACCTCCCCC TCTGGGCAAA
    AAAAGTGACC ATAGTAATGC CTTCTTCCCA AACAGCCCCT CCCCCTTTAC ACCACCTCCT
    CCTCAAACAC CTTCTCCTCA TGGAACAAGA AGGCATCTGC CGTCACCACC ACTGACACAA
    GAAGTGGACC TCCATTCCAT TGCTGGGCCG CCTGTTCCTC CACGACAAAG CACTTCTCAG
    CATATCCCTA AACTCCCTCC AAAAACTTAC AAAAGGGAGC ACACCCACCC GTCCATGCAC
    AGAGACGGGC CACCACTGTT GGAGAATGCC CATTCTTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691287.2
    CDS225..4226
    Translation

    Target ORF information:

    RefSeq Version XM_002691241.5
    Organism Bos taurus(cattle)
    Definition Bos taurus SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691241.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGGCGC AGCAGCTGCC GTACGAGTTT TTCAGCGAGG AGAACGCGCC CAAGTGGCGG 
    GGGCTGCTGG TGCCTGCGCT GAAAAAGGTT CAGGGGCAAG TTCATCCAAC TCTTGAGTCT
    AGTGATGATG CTCTTCAGTA TGTTGAAGAA TTAATTTTGC AGCTATTAAA TATGCTCTGC
    CAAGCTCAAC CCCGAAGTGC TTCAGATGTA GAGGAACGTG TTCAAAAAAG TTTCCCTCAT
    CCAATTGATA AGTGGGCAAT AGCTGATGCC CAATCGGCTA TTGAAAAGAG GAAGCGAAGA
    AACCCTTTAT CTCTCCCAGT AGAAAAAATC CATCCTTTGT TAAAGGAGGT CCTAGGTTAT
    AAAATTGACC ACCAGGTTTC TGTTTACATA GTAGCAGTAT TAGAATACAT TTCTGCAGAC
    ATTTTAAAGC TGGTGGGGAA TTATGTGCGA AATATACGGC ATTATGAAAT TACAAAACAA
    GATATTAAAG TGGCAATGTG TGCTGATAAG GTATTAATGG ATATGTTTCA TCAAGATGTG
    GAAGATATAA ATATATTATC TTTAACTGAT GAAGAGCCTT CCACCTCAGG AGAGCAGACT
    TACTATGATT TGGTAAAAGC ATTTATGGCA GAAATTCGAC AATACATAAG GGAACTTAAT
    TTAATTATAA AAGTTTTTAG AGAGCCCTTT GTCTCCAATT CAAAATTGTT TTCAGCTAAT
    GATGTAGAAA ATATATTTAG TCGTATAGTG GATATACATG AACTTAGTGT AAAATTACTG
    GGCCATATAG AAGATACTGT GGAAATGACA GATGAAGGCA GTCCCCATCC ATTAGTAGGA
    AGCTGCTTTG AAGACTTAGC GGAGGAACTG GCATTTGATC CATATGAATC ATATGCTCGA
    GATATTCTAC GACCTGGTTT TCACGATCGT TTCCTTAGTC AGTTATCAAA GCCTGGAGCA
    GCGCTCTATT TGCAGTCAAT AGGCGAAGGT TTCAAAGAAG CTGTTCAATA TGTTTTACCC
    AGACTACTTC TAGCCCCTGT TTACCACTGT CTGCATTACT TCGAACTTCT GAAGCAGTTA
    GAAGAAAAGA GTGAGGATCA AGAAGACAAA GAATGTTTGA AACAAGCAAT AACAGCTTTG
    CTTAATGTTC AGAGTGGTAT GGAAAAAATA TGTTCTAAAA GTCTTGCAAA ACGAAGACTT
    AGTGAATCTG CATGTCGGTT TTATAGTCAG CAAATGAAGG GGAAACAACT AGCAATCAAG
    AAAATGAACG AGATTCAGAA GAATATTGAT GGTTGGGAGG GAAAAGACAT TGGACAGTGT
    TGCAATGAGT TTATAATGGA AGGAACTCTT ACACGTGTAG GAGCCAAACA TGAGAGACAC
    ATATTTCTCT TTGATGGCTT AATGATTTGC TGTAAATCAA ATCATGGGCA GCCAAGACTA
    CCTGGTGCTA GCAATGCAGA ATATCGTCTT AAAGAAAAGT TTTTTATGAG AAAGGTACAA
    ATTAATGACA AAGATGACAC CAGTGAGTAC AAGCATGCTT TTGAAATAAT TTTAAAAGAT
    GAAAATAGTG TTATATTTTC TGCCAAGTCA GCTGAAGAGA AGAACAATTG GATGGCAGCA
    TTGATATCTT TACAGTACCG GAGTACCCTG GAAAGAATGC TTGATGTGAC CATGCTGCAG
    GAAGAAAAGG AGGAGCAGAT GAGGCTCCCT AGTGCTGACG TTTATAGATT TGCAGAGCCT
    GACTCTGAAG AAAATATCAT ATTTGAAGAA AACGTGCAGC CCAAAGCTGG AATTCCAATT
    ATCAAGGCAG GAACCGTTAT TAAACTTATA GAGAGGCTCA CATACCACAT GTATGCAGAT
    CCCAATTTTG TTCGGACATT TCTTACAACA TATAGATCCT TTTGTAAACC TCAAGAACTA
    CTGAGTCTTA TAATAGAAAG GTTTGAAATT CCAGAGCCTG AGCCAACAGA AGCTGATCGC
    ATCGCTATAG AGAATGGGGA TCAACCCTTG AGTGCAGAAC TAAAGAGATT TAGAAAAGAA
    TATATACAGC CTGTGCAGCT GCGAGTATTA AATGTATGTC GGCACTGGGT AGAGCACCAC
    TTCTATGATT TTGAAAGAGA TGCAGATCTT TTGCAGCGAA TGGAGGAATT TATTGGAACC
    GTAAGAGGTA AAGCAATGAA AAAATGGGTT GAATCCATCA CTAAAATAAT CCAAAGGAAA
    AAAATTGCCA GAGACAATGG ACCAGGTCAT AATATTACAT TTCAGAGTTC ACCTCCTACG
    GTTGAGTGGC ATATAAGCAG ACCTGGGCAC ATCGAGACTT TTGACCTGCT CACCTTACAC
    CCAATAGAAA TTGCTCGACA ACTCACTTTA CTTGAATCAG ATCTATATCG AGCTGTACAG
    CCATCAGAAT TAGTTGGAAG TGTGTGGACA AAAGAAGACA AAGAAATTAA TTCTCCTAAT
    CTTCTGAAAA TGATCCGGCA CACCACTAAT CTCACTCTGT GGTTTGAGAA ATGTATTGTA
    GAAACTGAAA ACTTAGAAGA AAGAGTAGCT GTGGTGAGTC GAATAATTGA GATTCTACAA
    GTGTTCCAAG AGCTCAACAA CTTCAATGGT GTTCTTGAGG TTGTCAGTGC TATGAACTCA
    TCCCCTGTTT ACAGACTAGA CCACACGTTT GAGCAAATAC CAAGTCGCCA AAAGAAAATT
    TTAGAAGAAG CTCATGAACT AAGTGAAGAT CACTATAAGA AATATTTGGC AAAACTCAGG
    TCTATTAATC CACCATGTGT GCCTTTCTTT GGAATTTATC TCACTAATAT CTTGAAAACA
    GAAGAAGGCA ACCCTGAGGT CCTAAAACGG CATGGAAAAG AGCTTATAAA CTTTAGCAAA
    AGGAGGAAAG TAGCAGAAAT AACAGGCGAG ATCCAGCAGT ACCAAAATCA GCCTTACTGT
    TTACGAGTAG AGTCAGATAT CAAAAGGTTT TTTGAAAACT TGAATCCAAT GGGAAATAGC
    ATGGAAAAAG AATTTACGGA TTATCTTTTC AACAAATCCC TAGAAATAGA ACCACGAAAC
    CCTAAGCCTC TTCCAAGATT TCCAAAAAAA TACAACTATC CCCTAAAATC TCCTGGTGTT
    CGTCCATCAA ACCCAAGACC AGGTACTATG AGACATCCCA CACCTCTGCA GCAAGAGCCA
    AGGAAAATTA GTTATAGTAG GATCCCTGAA AGTGAAACAG AAAGTACAGC ATCTGCACCA
    AATTCTCCAA GGACACCGTT AACACCTCCT CCTGCTTCTG GTGCTTCTAG TACCACAGAT
    GTTTGCAGCG TATTTGATTC TGATCATTCA AGCCCTTTTC ACTCAAGCAG CGATACCGTC
    TTTATCCAAG TTACACTGCC CCATGGCCCA AGATCTGCTT CAGTATCATC TATAAGTTTA
    ACCAAGAGCA CTGATGAAGT GCCTGTCCCC CCTCCTGTTC CTCCACGAAG ACGGCCAGAG
    TCTGCCCCAG CGGAATCTTC ACCATCTAAG ATGATGTCTA AGCATTTGGA CAGCCCCCCA
    GCAATTCCTC CTAGGCAACC CACATCAAAA GCCTATTCAC CACGGTATTC AATATCAGAC
    CGGACCTCTA TATCAGACCC TCCTGAAAGC CCTCCCTTAT TACCTCCACG AGAACCTGTG
    AGGACACCTG ATGTTTTCTC AAGTTCACCA CTACATCTCC AACCTCCCCC TCTGGGCAAA
    AAAAGTGACC ATAGTAATGC CTTCTTCCCA AACAGCCCCT CCCCCTTTAC ACCACCTCCT
    CCTCAAACAC CTTCTCCTCA TGGAACAAGA AGGCATCTGC CGTCACCACC ACTGACACAA
    GAAGTGGACC TCCATTCCAT TGCTGGGCCG CCTGTTCCTC CACGACAAAG CACTTCTCAG
    CATATCCCTA AACTCCCTCC AAAAACTTAC AAAAGGGAGC ACACCCACCC GTCCATGCAC
    AGAGACGGGC CACCACTGTT GGAGAATGCC CATTCTTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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