PIWIL1 cDNA ORF clone, Bos taurus(cattle)

The following PIWIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIWIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa95430 NM_001315576.1
Latest version!
Bos taurus piwi like RNA-mediated gene silencing 1 (PIWIL1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa08986 XM_024977378.1
Latest version!
Bos taurus piwi like RNA-mediated gene silencing 1 (PIWIL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa224352 XM_024977377.1
Latest version!
Bos taurus piwi like RNA-mediated gene silencing 1 (PIWIL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa95430
    Clone ID Related Accession (Same CDS sequence) NM_001315576.1
    Accession Version NM_001315576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product piwi-like protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KP770144.1. ##Evidence-Data-START## Transcript exon combination :: KP770144.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACTGGGA GAGCCAGAGC GAGAGCAAGA GGAAGGGCTC GTGGTCAGGA GATGGTGCAG 
    CATGTGGGCG CCTCTGCGAG TCAGCAGCCT GGTTACATTC AGCCTCGACC TCAGCAGCCG
    CCAGCAGAGG GGGAATTAGT TGGCCGCGGA CGACAGAGAG GAACAGTGGG AGCAACAGCG
    AAGCCACAAG AACTCCAGAT ATCTGCTGGA TTCCAGGAGT TATCATTAGC AGAGAGAGGA
    GGTCGTCGTA GAGACTTTCA TGATCTTGGT GTGAATACAA GACAGAACCT AGATCATGTG
    AAAGAATCAA AAACAGGTTC TTCAGGTATT ATCGTAAGGC TAAGCACTAA CCATTTCCGA
    TTGACATCCC GTCCCCAGTG GGCCTTATAT CAGTATCACA TTGACTATAA TCCGTTGATG
    GAAGCCAGAA GACTCCGCTC AGCTCTTCTT TTTCAACATG AGGATTTAAT TGGAAGGTGT
    CATGCTTTTG ATGGAACAAT ATTATTTTTA CCTAAAAGAC TACAGCACAA GGTTACTGAA
    GTTTTTAGTC AGACTCGAAA TGGGGAGCAC GTGAGGATAA CAATCACCCT GACAAATGAA
    CTCCCACCTA CGTCCCCAAC TTGTTTGCAG TTCTATAATA TTATTTTCAG GAGGCTTTTG
    AAGATCATGA ATTTGCAACA AATTGGACGG AATTACTATA ACCCAAGTGA CCCAATTGAT
    ATTCCAAATC ACAGATTGGT GATTTGGCCT GGCTTCACTA CTTCTATTCT TCAGTATGAA
    AATAACATCA TGCTGTGCAC TGATGTCAGT CATAAAGTTC TTCGAAGTGA AACTGTTCTA
    GATTTCATGT TCAACTTGTT TCATCAAACC GAAGAACACA AATTTCAAGA ACAAGTGTCT
    AAGGAACTGA TTGGTTTAAT TGTTCTCACC AAGTATAACA ACAAAACATA CAGAGTAGAT
    GATATTGACT GGGACCAGAA TCCAAAGAGT ACCTTTAAGA AAGCAGATGG CTCTGAAGTC
    AGTTTCTTAG AGTACTACAG GAAGCAATAT AACCAAGAAA TCACTGACTT GAAACAGCCA
    GTTTTGGTCA GTCAGCCTAA GAGAAGGAGA GGCCCTGGCG GCACATTACC AGGCCCAGCC
    ATGCTCATCC CTGAGCTCTG CTACCTCACA GGTTTAACTG ATAAAATGCG TAATGATTTT
    AATGTGATGA AAGACTTGGC TGTTCATACA AGACTGACTC CAGAGCAAAG GCAGCGTGAA
    GTAGGAAGAC TGATTGATTA TATTCATAAA GATGATAATG TGCAGAGGGA GCTTCGAGAC
    TGGGGTTTGA GCTTTGACTC CAACTTACTG TCCTTCTCAG GAAGAATTTT GCAAGCAGAA
    AAGATTCATC AAGGTGGAAA AACATTTGAT TACAATCCAC AATTTGCAGA TTGGTCAAAA
    GAAACAAGAG GTGCACCATT AATTAGTGTT AAGCCACTAG ATAACTGGTT GTTGATCTAT
    ACTCGAAGAA ATTATGAAGC AGCCAATTCG TTGATACAAA ATCTATTTAA AGTTACACCA
    GCCATGGGCA TACAAATGAA AAAAGCAATA ATGATTGAAG TGGATGATAG AACTGAAGCC
    TATTTAAGAG TCTTACAGCA AAAGGTTACG TCAGATACCC AGATAGTTGT TTGTCTGTTA
    TCAAGTAATC GGAAAGACAA ATATGATGCT ATTAAAAAAT ACTTATGTAC AGATTGCCCT
    ACTCCAAGTC AGTGTGTGGT GGCCCGAACC TTAGGCAAAC AACAGACTGT CATGGCCATT
    GCTACAAAGA TTGCGCTGCA GATGAACTGC AAGATGGGAG GAGAGCTTTG GAGGGTTGAT
    ATGCCTCTGA AGCTAGCGAT GATAGTTGGC ATTGATTGTT ACCATGATAC CACAGCTGGA
    CGGAGGTCAA TTGCAGGATT TGTGGCAAGC ATCAATGAAG GGATGACTCG CTGGTTCTCT
    CGCTGTGTGT TTCAAGACAG AGGACAAGAA CTAGTAGATG GGCTTAAGGT CTGCTTACAA
    GCTGCTCTAA GGGCTTGGAA TAGCTGCAAT GAATACATGC CTAGTCGAAT TATTGTGTAT
    CGGGATGGCG TAGGAGACGG TCAGCTTAAA ACACTGGTGA ACTATGAAGT ACCACAGTTT
    TTGGATTGCC TGAAATCTGT TGGTAGAGGT TACAACCCAA GACTAACGGT AATTGTGGTG
    AAGAAACGTG TCAACGCCAG ATTTTTTGCT CAGTCTGGAG GAAGACTTCA GAATCCACTT
    CCTGGAACAG TTATTGATGT AGAGGTTACA AGACCAGAAT GGTATGATTT TTTTATCGTG
    AGCCAGGCAG TGAGAAGTGG TAGTGTTTCT CCCACACATT ATAATGTCAT CTATGACAGC
    AGTGGCCTGA AGCCAGACCA CATACAGAGG TTAACTTACA AGCTGTGTCA TGTCTATTAT
    AACTGGCCAG GTGTCATTCG TGTTCCTGCT CCTTGCCAGT ATGCCCACAA ACTGGCTTTC
    CTTGTTGGCC AGAGTATTCA TAGAGAACCA AATCTGTCAC TGTCAAATCG TCTTTACTAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001302505.1
    CDS8..2593
    Translation

    Target ORF information:

    RefSeq Version NM_001315576.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piwi like RNA-mediated gene silencing 1 (PIWIL1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001315576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACTGGGA GAGCCAGAGC GAGAGCAAGA GGAAGGGCTC GTGGTCAGGA GATGGTGCAG 
    CATGTGGGCG CCTCTGCGAG TCAGCAGCCT GGTTACATTC AGCCTCGACC TCAGCAGCCG
    CCAGCAGAGG GGGAATTAGT TGGCCGCGGA CGACAGAGAG GAACAGTGGG AGCAACAGCG
    AAGCCACAAG AACTCCAGAT ATCTGCTGGA TTCCAGGAGT TATCATTAGC AGAGAGAGGA
    GGTCGTCGTA GAGACTTTCA TGATCTTGGT GTGAATACAA GACAGAACCT AGATCATGTG
    AAAGAATCAA AAACAGGTTC TTCAGGTATT ATCGTAAGGC TAAGCACTAA CCATTTCCGA
    TTGACATCCC GTCCCCAGTG GGCCTTATAT CAGTATCACA TTGACTATAA TCCGTTGATG
    GAAGCCAGAA GACTCCGCTC AGCTCTTCTT TTTCAACATG AGGATTTAAT TGGAAGGTGT
    CATGCTTTTG ATGGAACAAT ATTATTTTTA CCTAAAAGAC TACAGCACAA GGTTACTGAA
    GTTTTTAGTC AGACTCGAAA TGGGGAGCAC GTGAGGATAA CAATCACCCT GACAAATGAA
    CTCCCACCTA CGTCCCCAAC TTGTTTGCAG TTCTATAATA TTATTTTCAG GAGGCTTTTG
    AAGATCATGA ATTTGCAACA AATTGGACGG AATTACTATA ACCCAAGTGA CCCAATTGAT
    ATTCCAAATC ACAGATTGGT GATTTGGCCT GGCTTCACTA CTTCTATTCT TCAGTATGAA
    AATAACATCA TGCTGTGCAC TGATGTCAGT CATAAAGTTC TTCGAAGTGA AACTGTTCTA
    GATTTCATGT TCAACTTGTT TCATCAAACC GAAGAACACA AATTTCAAGA ACAAGTGTCT
    AAGGAACTGA TTGGTTTAAT TGTTCTCACC AAGTATAACA ACAAAACATA CAGAGTAGAT
    GATATTGACT GGGACCAGAA TCCAAAGAGT ACCTTTAAGA AAGCAGATGG CTCTGAAGTC
    AGTTTCTTAG AGTACTACAG GAAGCAATAT AACCAAGAAA TCACTGACTT GAAACAGCCA
    GTTTTGGTCA GTCAGCCTAA GAGAAGGAGA GGCCCTGGCG GCACATTACC AGGCCCAGCC
    ATGCTCATCC CTGAGCTCTG CTACCTCACA GGTTTAACTG ATAAAATGCG TAATGATTTT
    AATGTGATGA AAGACTTGGC TGTTCATACA AGACTGACTC CAGAGCAAAG GCAGCGTGAA
    GTAGGAAGAC TGATTGATTA TATTCATAAA GATGATAATG TGCAGAGGGA GCTTCGAGAC
    TGGGGTTTGA GCTTTGACTC CAACTTACTG TCCTTCTCAG GAAGAATTTT GCAAGCAGAA
    AAGATTCATC AAGGTGGAAA AACATTTGAT TACAATCCAC AATTTGCAGA TTGGTCAAAA
    GAAACAAGAG GTGCACCATT AATTAGTGTT AAGCCACTAG ATAACTGGTT GTTGATCTAT
    ACTCGAAGAA ATTATGAAGC AGCCAATTCG TTGATACAAA ATCTATTTAA AGTTACACCA
    GCCATGGGCA TACAAATGAA AAAAGCAATA ATGATTGAAG TGGATGATAG AACTGAAGCC
    TATTTAAGAG TCTTACAGCA AAAGGTTACG TCAGATACCC AGATAGTTGT TTGTCTGTTA
    TCAAGTAATC GGAAAGACAA ATATGATGCT ATTAAAAAAT ACTTATGTAC AGATTGCCCT
    ACTCCAAGTC AGTGTGTGGT GGCCCGAACC TTAGGCAAAC AACAGACTGT CATGGCCATT
    GCTACAAAGA TTGCGCTGCA GATGAACTGC AAGATGGGAG GAGAGCTTTG GAGGGTTGAT
    ATGCCTCTGA AGCTAGCGAT GATAGTTGGC ATTGATTGTT ACCATGATAC CACAGCTGGA
    CGGAGGTCAA TTGCAGGATT TGTGGCAAGC ATCAATGAAG GGATGACTCG CTGGTTCTCT
    CGCTGTGTGT TTCAAGACAG AGGACAAGAA CTAGTAGATG GGCTTAAGGT CTGCTTACAA
    GCTGCTCTAA GGGCTTGGAA TAGCTGCAAT GAATACATGC CTAGTCGAAT TATTGTGTAT
    CGGGATGGCG TAGGAGACGG TCAGCTTAAA ACACTGGTGA ACTATGAAGT ACCACAGTTT
    TTGGATTGCC TGAAATCTGT TGGTAGAGGT TACAACCCAA GACTAACGGT AATTGTGGTG
    AAGAAACGTG TCAACGCCAG ATTTTTTGCT CAGTCTGGAG GAAGACTTCA GAATCCACTT
    CCTGGAACAG TTATTGATGT AGAGGTTACA AGACCAGAAT GGTATGATTT TTTTATCGTG
    AGCCAGGCAG TGAGAAGTGG TAGTGTTTCT CCCACACATT ATAATGTCAT CTATGACAGC
    AGTGGCCTGA AGCCAGACCA CATACAGAGG TTAACTTACA AGCTGTGTCA TGTCTATTAT
    AACTGGCCAG GTGTCATTCG TGTTCCTGCT CCTTGCCAGT ATGCCCACAA ACTGGCTTTC
    CTTGTTGGCC AGAGTATTCA TAGAGAACCA AATCTGTCAC TGTCAAATCG TCTTTACTAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08986
    Clone ID Related Accession (Same CDS sequence) XM_024977378.1
    Accession Version XM_024977378.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piwi-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACTGGGA GAGCCAGAGC GAGAGCAAGA GGAAGGGCTC GTGGTCAGGA GATGGTGCAG 
    CATGTGGGCG CCTCTGCGAG TCAGCAGCCT GGTTACATTC AGCCTCGACC TCAGCAGCCG
    CCAGCAGAGG GGGAATTAGT TGGCCGCGGA CGACAGAGAG GAACAGTGGG AGCAACAGCG
    AAGTCACAAG AACTCCAGAT ATCTGCTGGA TTCCAGGAGT TATCATTAGC AGAGAGAGGA
    GGTCGTCGTA GAGACTTTCA TGATCTTGGT GTGAATACAA GACAGAACCT AGATCATGTG
    AAAGAATCAA AAACAGGTTC TTCAGGTATT ATCGTAAGGC TAAGCACTAA CCATTTCCGA
    TTGACATCCC GTCCCCAGTG GGCCTTATAT CAGTATCACA TTGACTATAA TCCGTTGATG
    GAAGCCAGAA GACTCCGCTC AGCTCTTCTT TTTCAACATG AGGATTTAAT TGGAAGGTGT
    CATGCTTTTG ATGGAACAAT ATTATTTTTA CCTAAAAGAC TACAGCACAA GGTTACTGAA
    GTTTTTAGTC AGACTCGAAA TGGAGAGCAC GTGAGGATAA CAATCACCCT GACAAATGAA
    CTCCCACCTA CGTCCCCAAC TTGTTTGCAG TTCTATAATA TTATTTTCAG GAGGCTTTTG
    AAGATCATGA ATTTGCAACA AATTGGACGG AATTACTATA ACCCAAGTGA CCCAATTGAT
    ATTCCAAATC ACAGATTGGT GATTTGGCCT GGCTTCACTA CTTCTATTCT TCAGTATGAA
    AATAACATCA TGCTGTGCAC TGATGTCAGT CATAAAGTTC TTCGAAGTGA AACTGTTCTA
    GATTTCATGT TCAACTTGTT TCATCAAACC GAAGAACACA AATTTCAAGA ACAAGTGTCT
    AAGGAACTGA TTGGTTTAAT TGTTCTCACC AAGTATAACA ACAAAACATA CAGAGTAGAT
    GATATTGACT GGGACCAGAA TCCAAAGAGT ACCTTTAAGA AAGCAGATGG CTCTGAAGTC
    AGTTTCTTAG AGTACTACAG GAAGCAATAT AACCAAGAAA TCACTGACTT GAAACAGCCA
    GTTTTGGTCA GTCAGCCTAA GAGAAGGAGA GGCCCTGGCG GCACATTACC AGGCCCAGCC
    ATGCTCATCC CTGAGCTCTG CTACCTCACA GGTTTAACTG ATAAAATGCG TAATGATTTT
    AATGTGATGA AAGACTTGGC TGTTCATACA AGACTGACTC CAGAGCAAAG GCAGCGTGAA
    GTAGGAAGAC TGATTGATTA TATTCATAAA GATGATAATG TGCAGAGGGA GCTTCGAGAC
    TGGGGTTTGA GCTTTGACTC CAACTTACTG TCCTTCTCAG GAAGAATTTT GCAAGCAGAA
    AAGATTCATC AAGGTGGAAA AACATTTGAT TACAATCCAC AATTTGCAGA TTGGTCAAAA
    GAAACAAGAG GTGCACCATT AATTAGTGTT AAGCCACTAG ATAACTGGTT GTTGATCTAT
    ACTCGAAGAA ATTATGAAGC AGCCAATTCG TTGATACAAA ATCTATTTAA AGTTACACCA
    GCCATGGGCA TACAAATGAA AAAAGCAATA ATGATTGAAG TGGATGATAG AACTGAAGCC
    TATTTAAGAG TCTTACAGCA AAAGGTTACG TCAGATACCC AGATAGTTGT TTGTCTGTTA
    TCAAGTAATC GGAAAGACAA ATATGATGCT ATTAAAAAAT ACTTATGTAC AGATTGCCCT
    ACTCCAAGTC AGTGTGTGGT GGCCCGAACC TTAGGCAAAC AACAGACTGT CATGGCCATT
    GCTACAAAGA TTGCGCTGCA GATGAACTGC AAGATGGGAG GAGAGCTTTG GAGGGTTGAT
    ATGCCTCTGA AGCTAGCGAT GATAGTTGGC ATTGATTGTT ACCATGATAC CACAGCTGGA
    CGGAGGTCAA TTGCAGGATT TGTGGCAAGC ATCAATGAAG GGATGACTCG CTGGTTCTCT
    CGCTGTGTGT TTCAAGACAG AGGACAAGAA CTAGTAGATG GGCTTAAGGT CTGCTTACAA
    GCTGCTCTAA GGGCTTGGAA TAGCTGCAAT GAATACATGC CTAGTCGAAT TATTGTGTAT
    CGGGATGGCG TAGGAGACGG TCAGCTTAAA ACACTGGTGA ACTATGAAGT ACCACAGTTT
    TTGGATTGCC TGAAATCTGT TGGTAGAGGT TACAACCCAA GACTAACGGT AATTGTGGTG
    AAGAAACGTG TCAACGCCAG ATTTTTTGCT CAGTCTGGAG GAAGACTTCA GAATCCACTT
    CCTGGAACAG TTATTGATGT AGAGGTTACA AGACCAGAAT GGTATGATTT TTTTATCGTG
    AGCCAGGCAG TGAGAAGTGG TAGTGTTTCT CCCACACATT ATAATGTCAT CTATGACAGC
    AGTGGCCTGA AGCCAGACCA CATACAGAGG TTAACTTACA AGCTGTGTCA TGTCTATTAT
    AACTGGCCAG GTGTCATTCG TGTTCCTGCT CCTTGCCAGT ATGCCCACAA ACTGGCTTTC
    CTTGTTGGCC AGAGTATTCA TAGAGAACCA AATCTGTCAC TGTCAAATCG TCTTTACTAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833146.1
    CDS279..2864
    Translation

    Target ORF information:

    RefSeq Version XM_024977378.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piwi like RNA-mediated gene silencing 1 (PIWIL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977378.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACTGGGA GAGCCAGAGC GAGAGCAAGA GGAAGGGCTC GTGGTCAGGA GATGGTGCAG 
    CATGTGGGCG CCTCTGCGAG TCAGCAGCCT GGTTACATTC AGCCTCGACC TCAGCAGCCG
    CCAGCAGAGG GGGAATTAGT TGGCCGCGGA CGACAGAGAG GAACAGTGGG AGCAACAGCG
    AAGTCACAAG AACTCCAGAT ATCTGCTGGA TTCCAGGAGT TATCATTAGC AGAGAGAGGA
    GGTCGTCGTA GAGACTTTCA TGATCTTGGT GTGAATACAA GACAGAACCT AGATCATGTG
    AAAGAATCAA AAACAGGTTC TTCAGGTATT ATCGTAAGGC TAAGCACTAA CCATTTCCGA
    TTGACATCCC GTCCCCAGTG GGCCTTATAT CAGTATCACA TTGACTATAA TCCGTTGATG
    GAAGCCAGAA GACTCCGCTC AGCTCTTCTT TTTCAACATG AGGATTTAAT TGGAAGGTGT
    CATGCTTTTG ATGGAACAAT ATTATTTTTA CCTAAAAGAC TACAGCACAA GGTTACTGAA
    GTTTTTAGTC AGACTCGAAA TGGAGAGCAC GTGAGGATAA CAATCACCCT GACAAATGAA
    CTCCCACCTA CGTCCCCAAC TTGTTTGCAG TTCTATAATA TTATTTTCAG GAGGCTTTTG
    AAGATCATGA ATTTGCAACA AATTGGACGG AATTACTATA ACCCAAGTGA CCCAATTGAT
    ATTCCAAATC ACAGATTGGT GATTTGGCCT GGCTTCACTA CTTCTATTCT TCAGTATGAA
    AATAACATCA TGCTGTGCAC TGATGTCAGT CATAAAGTTC TTCGAAGTGA AACTGTTCTA
    GATTTCATGT TCAACTTGTT TCATCAAACC GAAGAACACA AATTTCAAGA ACAAGTGTCT
    AAGGAACTGA TTGGTTTAAT TGTTCTCACC AAGTATAACA ACAAAACATA CAGAGTAGAT
    GATATTGACT GGGACCAGAA TCCAAAGAGT ACCTTTAAGA AAGCAGATGG CTCTGAAGTC
    AGTTTCTTAG AGTACTACAG GAAGCAATAT AACCAAGAAA TCACTGACTT GAAACAGCCA
    GTTTTGGTCA GTCAGCCTAA GAGAAGGAGA GGCCCTGGCG GCACATTACC AGGCCCAGCC
    ATGCTCATCC CTGAGCTCTG CTACCTCACA GGTTTAACTG ATAAAATGCG TAATGATTTT
    AATGTGATGA AAGACTTGGC TGTTCATACA AGACTGACTC CAGAGCAAAG GCAGCGTGAA
    GTAGGAAGAC TGATTGATTA TATTCATAAA GATGATAATG TGCAGAGGGA GCTTCGAGAC
    TGGGGTTTGA GCTTTGACTC CAACTTACTG TCCTTCTCAG GAAGAATTTT GCAAGCAGAA
    AAGATTCATC AAGGTGGAAA AACATTTGAT TACAATCCAC AATTTGCAGA TTGGTCAAAA
    GAAACAAGAG GTGCACCATT AATTAGTGTT AAGCCACTAG ATAACTGGTT GTTGATCTAT
    ACTCGAAGAA ATTATGAAGC AGCCAATTCG TTGATACAAA ATCTATTTAA AGTTACACCA
    GCCATGGGCA TACAAATGAA AAAAGCAATA ATGATTGAAG TGGATGATAG AACTGAAGCC
    TATTTAAGAG TCTTACAGCA AAAGGTTACG TCAGATACCC AGATAGTTGT TTGTCTGTTA
    TCAAGTAATC GGAAAGACAA ATATGATGCT ATTAAAAAAT ACTTATGTAC AGATTGCCCT
    ACTCCAAGTC AGTGTGTGGT GGCCCGAACC TTAGGCAAAC AACAGACTGT CATGGCCATT
    GCTACAAAGA TTGCGCTGCA GATGAACTGC AAGATGGGAG GAGAGCTTTG GAGGGTTGAT
    ATGCCTCTGA AGCTAGCGAT GATAGTTGGC ATTGATTGTT ACCATGATAC CACAGCTGGA
    CGGAGGTCAA TTGCAGGATT TGTGGCAAGC ATCAATGAAG GGATGACTCG CTGGTTCTCT
    CGCTGTGTGT TTCAAGACAG AGGACAAGAA CTAGTAGATG GGCTTAAGGT CTGCTTACAA
    GCTGCTCTAA GGGCTTGGAA TAGCTGCAAT GAATACATGC CTAGTCGAAT TATTGTGTAT
    CGGGATGGCG TAGGAGACGG TCAGCTTAAA ACACTGGTGA ACTATGAAGT ACCACAGTTT
    TTGGATTGCC TGAAATCTGT TGGTAGAGGT TACAACCCAA GACTAACGGT AATTGTGGTG
    AAGAAACGTG TCAACGCCAG ATTTTTTGCT CAGTCTGGAG GAAGACTTCA GAATCCACTT
    CCTGGAACAG TTATTGATGT AGAGGTTACA AGACCAGAAT GGTATGATTT TTTTATCGTG
    AGCCAGGCAG TGAGAAGTGG TAGTGTTTCT CCCACACATT ATAATGTCAT CTATGACAGC
    AGTGGCCTGA AGCCAGACCA CATACAGAGG TTAACTTACA AGCTGTGTCA TGTCTATTAT
    AACTGGCCAG GTGTCATTCG TGTTCCTGCT CCTTGCCAGT ATGCCCACAA ACTGGCTTTC
    CTTGTTGGCC AGAGTATTCA TAGAGAACCA AATCTGTCAC TGTCAAATCG TCTTTACTAC
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224352
    Clone ID Related Accession (Same CDS sequence) XM_024977377.1
    Accession Version XM_024977377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piwi-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACTGGGA GAGCCAGAGC GAGAGCAAGA GGAAGGGCTC GTGGTCAGGA GATGGTGCAG 
    CATGTGGGCG CCTCTGCGAG TCAGCAGCCT GGTTACATTC AGCCTCGACC TCAGCAGCCG
    CCAGCAGAGG GGGAATTAGT TGGCCGCGGA CGACAGAGAG GAACAGTGGG AGCAACAGCG
    AAGTCACAAG AACTCCAGAT ATCTGCTGGA TTCCAGGAGT TATCATTAGC AGAGAGAGGA
    GGTCGTCGTA GAGACTTTCA TGATCTTGGT GTGAATACAA GACAGAACCT AGATCATGTG
    AAAGAATCAA AAACAGGTTC TTCAGGTATT ATCGTAAGGC TAAGCACTAA CCATTTCCGA
    TTGACATCCC GTCCCCAGTG GGCCTTATAT CAGTATCACA TTGACTATAA TCCGTTGATG
    GAAGCCAGAA GACTCCGCTC AGCTCTTCTT TTTCAACATG AGGATTTAAT TGGAAGGTGT
    CATGCTTTTG ATGGAACAAT ATTATTTTTA CCTAAAAGAC TACAGCACAA GGTTACTGAA
    GTTTTTAGTC AGACTCGAAA TGGAGAGCAC GTGAGGATAA CAATCACCCT GACAAATGAA
    CTCCCACCTA CGTCCCCAAC TTGTTTGCAG TTCTATAATA TTATTTTCAG GAGGCTTTTG
    AAGATCATGA ATTTGCAACA AATTGGACGG AATTACTATA ACCCAAGTGA CCCAATTGAT
    ATTCCAAATC ACAGATTGGT GATTTGGCCT GGCTTCACTA CTTCTATTCT TCAGTATGAA
    AATAACATCA TGCTGTGCAC TGATGTCAGT CATAAAGTTC TTCGAAGTGA AACTGTTCTA
    GATTTCATGT TCAACTTGTT TCATCAAACC GAAGAACACA AATTTCAAGA ACAAGTGTCT
    AAGGAACTGA TTGGTTTAAT TGTTCTCACC AAGTATAACA ACAAAACATA CAGAGTAGAT
    GATATTGACT GGGACCAGAA TCCAAAGAGT ACCTTTAAGA AAGCAGATGG CTCTGAAGTC
    AGTTTCTTAG AGTACTACAG GAAGCAATAT AACCAAGAAA TCACTGACTT GAAACAGCCA
    GTTTTGGTCA GTCAGCCTAA GAGAAGGAGA GGCCCTGGCG GCACATTACC AGGCCCAGCC
    ATGCTCATCC CTGAGCTCTG CTACCTCACA GGTTTAACTG ATAAAATGCG TAATGATTTT
    AATGTGATGA AAGACTTGGC TGTTCATACA AGACTGACTC CAGAGCAAAG GCAGCGTGAA
    GTAGGAAGAC TGATTGATTA TATTCATAAA GATGATAATG TGCAGAGGGA GCTTCGAGAC
    TGGGGTTTGA GCTTTGACTC CAACTTACTG TCCTTCTCAG GAAGAATTTT GCAAGCAGAA
    AAGATTCATC AAGGTGGAAA AACATTTGAT TACAATCCAC AATTTGCAGA TTGGTCAAAA
    GAAACAAGAG GTGCACCATT AATTAGTGTT AAGCCACTAG ATAACTGGTT GTTGATCTAT
    ACTCGAAGAA ATTATGAAGC AGCCAATTCG TTGATACAAA ATCTATTTAA AGTTACACCA
    GCCATGGGCA TACAAATGAA AAAAGCAATA ATGATTGAAG TGGATGATAG AACTGAAGCC
    TATTTAAGAG TCTTACAGCA AAAGGTTACG TCAGATACCC AGATAGTTGT TTGTCTGTTA
    TCAAGTAATC GGAAAGACAA ATATGATGCT ATTAAAAAAT ACTTATGTAC AGATTGCCCT
    ACTCCAAGTC AGTGTGTGGT GGCCCGAACC TTAGGCAAAC AACAGACTGT CATGGCCATT
    GCTACAAAGA TTGCGCTGCA GATGAACTGC AAGATGGGAG GAGAGCTTTG GAGGGTTGAT
    ATGCCTCTGA AGCTAGCGAT GATAGTTGGC ATTGATTGTT ACCATGATAC CACAGCTGGA
    CGGAGGTCAA TTGCAGGATT TGTGGCAAGC ATCAATGAAG GGATGACTCG CTGGTTCTCT
    CGCTGTGTGT TTCAAGACAG AGGACAAGAA CTAGTAGATG GGCTTAAGGT CTGCTTACAA
    GCTGCTCTAA GGGCTTGGAA TAGCTGCAAT GAATACATGC CTAGTCGAAT TATTGTGTAT
    CGGGATGGCG TAGGAGACGG TCAGCTTAAA ACACTGGTGA ACTATGAAGT ACCACAGTTT
    TTGGATTGCC TGAAATCTGT TGGTAGAGGT TACAACCCAA GACTAACGGT AATTGTGGTG
    AAGAAACGTG TCAACGCCAG ATTTTTTGCT CAGTCTGGAG GAAGACTTCA GAATCCACTT
    CCTGGAACAG TTATTGATGT AGAGGTTACA AGACCAGAAT GGGTGTCATT CGTGTTCCTG
    CTCCTTGCCA GTATGCCCAC AAACTGGCTT TCCTTGTTGG CCAGAGTATT CATAGAGAAC
    CAAATCTGTC ACTGTCAAAT CGTCTTTACT ACCTCTAACC TACAGAAGAC GACAAGATGT
    GGCTGCGTTT TTCTTTTTGA AATTATATCT TCAGGATTTT TTTTTAAGCT CTTATTTTTT
    AAACTGTTAT CTTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833145.1
    CDS84..2621
    Translation

    Target ORF information:

    RefSeq Version XM_024977377.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piwi like RNA-mediated gene silencing 1 (PIWIL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACTGGGA GAGCCAGAGC GAGAGCAAGA GGAAGGGCTC GTGGTCAGGA GATGGTGCAG 
    CATGTGGGCG CCTCTGCGAG TCAGCAGCCT GGTTACATTC AGCCTCGACC TCAGCAGCCG
    CCAGCAGAGG GGGAATTAGT TGGCCGCGGA CGACAGAGAG GAACAGTGGG AGCAACAGCG
    AAGTCACAAG AACTCCAGAT ATCTGCTGGA TTCCAGGAGT TATCATTAGC AGAGAGAGGA
    GGTCGTCGTA GAGACTTTCA TGATCTTGGT GTGAATACAA GACAGAACCT AGATCATGTG
    AAAGAATCAA AAACAGGTTC TTCAGGTATT ATCGTAAGGC TAAGCACTAA CCATTTCCGA
    TTGACATCCC GTCCCCAGTG GGCCTTATAT CAGTATCACA TTGACTATAA TCCGTTGATG
    GAAGCCAGAA GACTCCGCTC AGCTCTTCTT TTTCAACATG AGGATTTAAT TGGAAGGTGT
    CATGCTTTTG ATGGAACAAT ATTATTTTTA CCTAAAAGAC TACAGCACAA GGTTACTGAA
    GTTTTTAGTC AGACTCGAAA TGGAGAGCAC GTGAGGATAA CAATCACCCT GACAAATGAA
    CTCCCACCTA CGTCCCCAAC TTGTTTGCAG TTCTATAATA TTATTTTCAG GAGGCTTTTG
    AAGATCATGA ATTTGCAACA AATTGGACGG AATTACTATA ACCCAAGTGA CCCAATTGAT
    ATTCCAAATC ACAGATTGGT GATTTGGCCT GGCTTCACTA CTTCTATTCT TCAGTATGAA
    AATAACATCA TGCTGTGCAC TGATGTCAGT CATAAAGTTC TTCGAAGTGA AACTGTTCTA
    GATTTCATGT TCAACTTGTT TCATCAAACC GAAGAACACA AATTTCAAGA ACAAGTGTCT
    AAGGAACTGA TTGGTTTAAT TGTTCTCACC AAGTATAACA ACAAAACATA CAGAGTAGAT
    GATATTGACT GGGACCAGAA TCCAAAGAGT ACCTTTAAGA AAGCAGATGG CTCTGAAGTC
    AGTTTCTTAG AGTACTACAG GAAGCAATAT AACCAAGAAA TCACTGACTT GAAACAGCCA
    GTTTTGGTCA GTCAGCCTAA GAGAAGGAGA GGCCCTGGCG GCACATTACC AGGCCCAGCC
    ATGCTCATCC CTGAGCTCTG CTACCTCACA GGTTTAACTG ATAAAATGCG TAATGATTTT
    AATGTGATGA AAGACTTGGC TGTTCATACA AGACTGACTC CAGAGCAAAG GCAGCGTGAA
    GTAGGAAGAC TGATTGATTA TATTCATAAA GATGATAATG TGCAGAGGGA GCTTCGAGAC
    TGGGGTTTGA GCTTTGACTC CAACTTACTG TCCTTCTCAG GAAGAATTTT GCAAGCAGAA
    AAGATTCATC AAGGTGGAAA AACATTTGAT TACAATCCAC AATTTGCAGA TTGGTCAAAA
    GAAACAAGAG GTGCACCATT AATTAGTGTT AAGCCACTAG ATAACTGGTT GTTGATCTAT
    ACTCGAAGAA ATTATGAAGC AGCCAATTCG TTGATACAAA ATCTATTTAA AGTTACACCA
    GCCATGGGCA TACAAATGAA AAAAGCAATA ATGATTGAAG TGGATGATAG AACTGAAGCC
    TATTTAAGAG TCTTACAGCA AAAGGTTACG TCAGATACCC AGATAGTTGT TTGTCTGTTA
    TCAAGTAATC GGAAAGACAA ATATGATGCT ATTAAAAAAT ACTTATGTAC AGATTGCCCT
    ACTCCAAGTC AGTGTGTGGT GGCCCGAACC TTAGGCAAAC AACAGACTGT CATGGCCATT
    GCTACAAAGA TTGCGCTGCA GATGAACTGC AAGATGGGAG GAGAGCTTTG GAGGGTTGAT
    ATGCCTCTGA AGCTAGCGAT GATAGTTGGC ATTGATTGTT ACCATGATAC CACAGCTGGA
    CGGAGGTCAA TTGCAGGATT TGTGGCAAGC ATCAATGAAG GGATGACTCG CTGGTTCTCT
    CGCTGTGTGT TTCAAGACAG AGGACAAGAA CTAGTAGATG GGCTTAAGGT CTGCTTACAA
    GCTGCTCTAA GGGCTTGGAA TAGCTGCAAT GAATACATGC CTAGTCGAAT TATTGTGTAT
    CGGGATGGCG TAGGAGACGG TCAGCTTAAA ACACTGGTGA ACTATGAAGT ACCACAGTTT
    TTGGATTGCC TGAAATCTGT TGGTAGAGGT TACAACCCAA GACTAACGGT AATTGTGGTG
    AAGAAACGTG TCAACGCCAG ATTTTTTGCT CAGTCTGGAG GAAGACTTCA GAATCCACTT
    CCTGGAACAG TTATTGATGT AGAGGTTACA AGACCAGAAT GGGTGTCATT CGTGTTCCTG
    CTCCTTGCCA GTATGCCCAC AAACTGGCTT TCCTTGTTGG CCAGAGTATT CATAGAGAAC
    CAAATCTGTC ACTGTCAAAT CGTCTTTACT ACCTCTAACC TACAGAAGAC GACAAGATGT
    GGCTGCGTTT TTCTTTTTGA AATTATATCT TCAGGATTTT TTTTTAAGCT CTTATTTTTT
    AAACTGTTAT CTTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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