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IRS1 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol IRS1
Entrez Gene ID 538598
Full Name insulin receptor substrate 1
General protein information
Preferred Names

insulin receptor substrate 1

Names

insulin receptor substrate 1
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_003581871.4 XP_003581919.1 insulin receptor substrate 1
XM_003585773.4 XP_003585821.1 insulin receptor substrate 1
XM_003585773.5 XP_003585821.1 insulin receptor substrate 1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Irs1 NP_037101.1
Gallus gallus (chicken) IRS1 XP_004951158.1
Homo sapiens (human) IRS1 NP_005535.1
Pan troglodytes (chimpanzee) IRS1 XP_001134895.1
Xenopus tropicalis (tropical clawed frog) LOC100495320 XP_004914519.1
Canis lupus familiaris (dog) IRS1 XP_543274.3
Danio rerio (zebrafish) irs1 XP_005157831.1
Bos taurus (cattle) IRS1 XP_003585821.1
Mus musculus (house mouse) Irs1 NP_034700.2

Gene Ontology

The following IRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227808 XM_003585773.5
Latest version!		 Bos taurus insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OBa02991 XM_003585773.4
Latest version!		 Bos taurus insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OBa02991 XM_003581871.4
Latest version!		 Bos taurus insulin receptor substrate 1 (IRS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa227808
Clone ID Related Accession (Same CDS sequence) XM_003585773.5
Accession Version XM_003585773.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003585773.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAAGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003585821.1
CDS1142..4855
Translation

Target ORF information:

RefSeq Version XM_003585773.5
Organism Bos taurus(cattle)
Definition Bos taurus insulin receptor substrate 1 (IRS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003585773.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAAGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa02991
Clone ID Related Accession (Same CDS sequence) XM_003581871.4 , XM_003585773.4
Accession Version XM_003581871.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003581871.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003581919.1
CDS345..4058
Translation

Target ORF information:

RefSeq Version XM_003581871.4
Organism Bos taurus(cattle)
Definition Bos taurus insulin receptor substrate 1 (IRS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003581871.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa02991
Clone ID Related Accession (Same CDS sequence) XM_003581871.4 , XM_003585773.4
Accession Version XM_003585773.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003585773.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003585821.1
CDS345..4058
Translation

Target ORF information:

RefSeq Version XM_003585773.4
Organism Bos taurus(cattle)
Definition Bos taurus insulin receptor substrate 1 (IRS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003585773.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGAGCC CTCCGGAGAC TGACGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGATTCTTC GTGCTGCGGG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC TAGCGCCCCC 
AAACGCTCGA TCCCCCTGGA GAGCTGCTTC AACATCAACA AGCGGGCGGA CTCCAAGAAC 
AAGCACCTGG TGGCCCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC AGACAGCGAG 
GCCGAGCAGG ACAGCTGGTA CCAGGCCCTC CTGCAGCTGC ACAACCGTGC CAAGGGCCAC 
CACGACGGGG CCGCGGCGCC TGGGGCGGGA GGCGGCGGGG GCAGCTGCAG TGGCAGCTCC 
GGCCTCGGGG AGGCCGGTGA GGACTTGAGC TACGGGGACG TGCCCCCAGG ACCCGCCTTC 
AAGGAGGTCT GGCAGGTGAT CCTGAAACCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGCATCTACC GCCTCTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCTGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GCGGCCACTC AGAGAACTTC 
TTCTTCATCG AAGTGGGCCG TTCCGCAGTG ACGGGCCCCG GGGAGTTCTG GATGCAGGTG 
GACGACTCCG TGGTGGCCCA GAACATGCAC GAGACAATCC TGGAGGCCAT GCGGGCCATG 
AGCGATGAGT TCCGCCCTAG AAGCAAGAGC CAGTCCTCCT CCAACTGCTC CAACCCCATC 
AGTGTGCCCC TGCGGAGGCA CCACCTCAAC AACCCTCCAC CCAGCCAGGT GGGGCTGACC 
CGCCGCTCGC GCACCGAGAG CATCACAGCC ACCTCCCCGG CCAGCTTGGT GGGCGGGAAG 
CAGGGCTCCT TCCGCGTGCG TGCCTCCAGT GATGGCGAAG GCACCATGTC TCGCCCCGCC 
TCAGTGGACG GCAGTCCTGT GAGTCCTAGC ACCAACAGGA CCCACGCCCA CCGCCATCGA 
GGCAGCTCCC GGCTGCACCC GCCTCTCAAC CACAGCCGCT CCATCCCCAT GCCTTCTTCT 
CGCTGCTCGC CTTCCGCCAC CAGCCCCGTC AGTCTGTCGT CCAGCAGCAC GAGTGGCCAT 
GGCTCCACCT CAGACTGCCT CTTCCCGCGG CGGTCTAGTG CTTCTGTGTC TGGTTCCCCC 
AGTGATGGCG GCTTCATCTC CTCCGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGAAGT 
TCCTTCCGCA GCGTCACCCC AGATTCCCTG GGCCACACCC CGCCCGCCCG GGGTGAGGAG 
GAGCTAAGCA ACTACATCTG CATGGGAGGC AAGGGGGCCT CCACCCTCAC TGCCCCCAAT 
GGTCACTACA TTTTGCCCCG GGGTGGCAAT GGTCACCGCT ACGTGCCAGC CGCCGGCTTG 
GGCACGAGCC CAGCCCTGCC AGGAGAAGAA GCAGCCGGTG CGGCAGATCT GGATAATCGG 
TTCCGAAAAC GGACTCACTC TGCGGGCACT TCGCCTACCA TTTCCCACCA GAAAACCCCG 
TCTCAGTCCT CTGTGGCTTC CATTGAGGAA TATACCGAAA TGATGCCCGC TTACCCACCA 
GGAGGTGGCA GTGGAGGCCG AGTACCCAGC TACCGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GGCGTGGGGG CCACCACCGC 
CCAGACACCT CCAGCCTTCA CACTGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCG GCAGCCGGAA AGGCAGTGGG GACTACATGC CCATGAGCCC CAAGAGCGTG 
TCTGCCCCGC AGCAGATCAT CAACCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAC 
GGCTACATGA TGATGTCCCC AAGCGGCAGC TGCTCTCCTG ACATTGGAGG TGGGCCCAGC 
AGCGGTGGCA GCAGCAGCGG TGCCGCCCCT TCTGGGAGCA GCTATGGCAA GCTTTGGACA 
AACGGGGTAG GGGGCCACCA CTCTCACGCC CTGCCACACC CCAAACTTCC GGTGGAGAGC 
GGTAGTGGCA AACTCTTGTC TTGTACAGGT GACTACATGA ACATGTCGCC CGTCGGGGAC 
TCCAACACCA GCAGCCCCTC CGACTGCTAT TATGGCCCCG AGGACCCCCA GCACAAGCCC 
GTCCTCTCCT ACTACTCATT GCCAAGGTCC TTTAAGCACA CCCAGCGTCC TGGGGAGCTG 
GAGGAGCCCC GGCACCACCA GCACCTCCGC CTTTCCTCAA GTTCCGGTCG ACTCCTCTAC 
ACTGCGGCCG CAGAAGATTC CTCTTCGTCC ACCAGCAGCG ACAGCCTGGG CGGGGGATAC 
TGTGGGGCTC GGCCGGAGCC CGGCCTCCCG CATCTCCATC ATCAGGTCCT GCAGGCCCAT 
CTGCCTCGAA AGGTGGACAC AGCTGCCCAG ACCAACAACC GCCTGGCTCG GCCCACGAGG 
CTGTCTCTGG GGGATCCCAA GGCCAGCACC TTACCTCGGG CCCGAGAGCA GCAGCCACCG 
CCCCCCTTGC TGCTCCCTCC GGAGCCCAAG AGCCCAGGGG AGTATGTGAA TATTGAATTT 
GGGAGCGATC AGCCAGGCTA CTTATCGGGC CCGGTGGCGT CCCACAGCTC GCCTTCCATC 
AGGTGTCCGT CCCAGCTCCA GCCAGCTCCC AGAGAGGAGG ATACGGGCGC AGAAGAGTAT 
ATGAACATGG ACCTGGGGCC GGGCCGGAGG GCCACCTGGC AGAAGAGCGT GGGGGTCCAG 
CCCGGCAGGG TGGGCCCGGC GCCCCCTGGA GCTGCTAGCG TGTGCAGGCC GACTAGGGCA 
GTGCCCAGCA GCTGCGGCGA CTACATGACC ATGCAGATGG GTGGTCCCCG GCAGAGCTAC 
GTGGACACCT CACCTGTCGC GCCCATCAGC TACGCCGACA TGCGGACGGG CGTCGTTGTG 
GAGGATGCCA GCTTGCCCAG GGCCACCGCG GCCGCTCCCT CCTCATCTTC GACAGCCTCT 
GTTTCCTCCA CTGCGCCTCC TCCGGGAACA GGGGAGCTGG CGGCCCGCTC GGCCCTCCTG 
GGGGGCCCGA GCGCCTTCAC GCGGGTGAAC CTCAGTCCCA ACCGCAACCA GAGTGCCAAA 
GTGATCCGTG CCGACCCTCA AGGGTGCAGG AGGCGGCACA GCTCCGAGAC CTTCTCCTCT 
ACGCCCAGTG CCACCCGGGT GGGCAACACG ATGCCCTTCG GAGCTGGGGC TGCCGTCGGG 
GGCAGCGGTG GCGGCAGCAG CAGCAGCGCC GAGGATGTCA AACGCCACAG CTCTGCTTCC 
TTCGAGAACG TGTGGCTGAG GCCTGGGGAG CTCGGGGGAG CCCCCAAGGA GCTGGCCCAA 
GTGTGCGGGG CCGCTGGGGG TTTGGAGAAT GGTCTTAACT ACATAGACCT GGATTTGGTC 
AAGGACTTCA AACAGCGCCC TCAAGAGTGC CCACCTCAAC CGCAGCCTCC TCCACTCCCG 
GCCCCTCATC AGCCTCTGGC CAGTAGTCAG AGCGGCCCCA CCAGCCGCTC CAGCGAGGAT 
CTAAGCGCCT ATGCCAGCAT CACTTTCCAG AAGCAGCCAG AGGACCTCCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed