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NKX2-1 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol NKX2-1
Entrez Gene ID 613713
Full Name NK2 homeobox 1
General protein information
Preferred Names

NK2 homeobox 1

Names

homeobox protein Nkx-2.1
NK2 homeobox 1-like
thyroid-specific transcription factor 1
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005222055.3 XP_005222112.1 homeobox protein Nkx-2.1 isoform X2
XM_005222055.4 XP_005222112.1 homeobox protein Nkx-2.1 isoform X1
NM_001191458.1 NP_001178387.1 homeobox protein Nkx-2.1
XM_010817163.2 XP_010815465.1 homeobox protein Nkx-2.1 isoform X1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Nkx2-1 NP_037225.1
Danio rerio (zebrafish) nkx2.1b NP_571851.1
Xenopus tropicalis (tropical clawed frog) nkx2-1 XP_002935383.1
Homo sapiens (human) NKX2-1 NP_001073136.1
Pan troglodytes (chimpanzee) NKX2-1 XP_509911.2
NKX2-1 XP_001089773.1
Bos taurus (cattle) LOC613713 NP_001178387.1
Mus musculus (house mouse) Nkx2-1 NP_001139670.1
Gallus gallus (chicken) NKX2-1 NP_989947.1

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NKX2-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKX2-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa38723 NM_001191458.1
Latest version!		 Bos taurus NK2 homeobox 1 (NKX2-1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $258.30
$369.00
OBa31006 XM_005222055.4
Latest version!		 Bos taurus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $258.30
$369.00
OBa38723 XM_010817163.2
Latest version!		 Bos taurus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $258.30
$369.00
View Old Accession Versions >>
OBa31006 XM_005222055.3 Bos taurus NK2 homeobox 1 (NKX2-1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa38723
Clone ID Related Accession (Same CDS sequence) NM_001191458.1 , XM_010817163.2
Accession Version NM_001191458.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-07-13
Organism Bos taurus(cattle)
Product homeobox protein Nkx-2.1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000021.1. On Jul 17, 2010 this sequence version replaced XM_002702671.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DV891443.1, EV635644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145444 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT 
TACAGGCAGG GCCAGACGGC ACCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCAG TCACCGCCGC CTACCACATG ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGCGGAACAG CGCCTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCTCTGGG GGACGTGAGC AAGAATATGG CCCCGCTGCC AAGCGCACCG 
CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG GCGCAGGTGT ATGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC CGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAAGCC 
AAGGACAAGG CGGCACAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG CGGGGGCGCA 
GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG 
GTCCTGGTGA AAGACGGCAA ACCTTGCCAG GCGGGCGCCC CCGCGCCGGG CGCCACCAGC 
CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG 
GCAGCTATTT CAGTGGGCAG CGGTGGCCCC GGCCTGGGTG CCCACCCGGG CCACCAGCCG 
GGCAGCGCGG GCCAGTCTCC GGACCTGGCG CACCACGCCG CCAGCCCCGC GGCGCTGCAG 
GGCCAGGTCT CCAGCCTGCC CCACCTGAAC TCCTCGGGCT CGGACTACGG CACCATGTCC 
TGCTCCACCT TGCTATACGG TCGGACCTGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001178387.1
CDS424..1536
Translation

Target ORF information:

RefSeq Version NM_001191458.1
Organism Bos taurus(cattle)
Definition Bos taurus NK2 homeobox 1 (NKX2-1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001191458.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT 
TACAGGCAGG GCCAGACGGC ACCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCAG TCACCGCCGC CTACCACATG ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGCGGAACAG CGCCTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCTCTGGG GGACGTGAGC AAGAATATGG CCCCGCTGCC AAGCGCACCG 
CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG GCGCAGGTGT ATGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC CGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAAGCC 
AAGGACAAGG CGGCACAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG CGGGGGCGCA 
GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG 
GTCCTGGTGA AAGACGGCAA ACCTTGCCAG GCGGGCGCCC CCGCGCCGGG CGCCACCAGC 
CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG 
GCAGCTATTT CAGTGGGCAG CGGTGGCCCC GGCCTGGGTG CCCACCCGGG CCACCAGCCG 
GGCAGCGCGG GCCAGTCTCC GGACCTGGCG CACCACGCCG CCAGCCCCGC GGCGCTGCAG 
GGCCAGGTCT CCAGCCTGCC CCACCTGAAC TCCTCGGGCT CGGACTACGG CACCATGTCC 
TGCTCCACCT TGCTATACGG TCGGACCTGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa31006
Clone ID Related Accession (Same CDS sequence) XM_005222055.3 , XM_005222055.4
Accession Version XM_005222055.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product homeobox protein Nkx-2.1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
GGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT TACAGGCAGG GCCAGACGGC ACCGCCGGCC 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCAG TCACCGCCGC CTACCACATG 
ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGCGGAACAG CGCCTCGGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCTCTGGG GGACGTGAGC 
AAGAATATGG CCCCGCTGCC AAGCGCACCG CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG 
GCGCAGGTGT ATGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC CGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACACCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAGATGAA GCGCCAAGCC AAGGACAAGG CGGCACAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG CGGGGGCGCA GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG 
CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG GTCCTGGTGA AAGACGGCAA ACCTTGCCAG 
GCGGGCGCCC CCGCGCCGGG CGCCACCAGC CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG 
CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG GCAGCTATTT CAGTGGGCAG CGGTGGCCCC 
GGCCTGGGTG CCCACCCGGG CCACCAGCCG GGCAGCGCGG GCCAGTCTCC GGACCTGGCG 
CACCACGCCG CCAGCCCCGC GGCGCTGCAG GGCCAGGTCT CCAGCCTGCC CCACCTGAAC 
TCCTCGGGCT CGGACTACGG CACCATGTCC TGCTCCACCT TGCTATACGG TCGGACCTGG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005222112.1
CDS273..1475
Translation

Target ORF information:

RefSeq Version XM_005222055.3
Organism Bos taurus(cattle)
Definition Bos taurus NK2 homeobox 1 (NKX2-1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005222055.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
GGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT TACAGGCAGG GCCAGACGGC ACCGCCGGCC 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCAG TCACCGCCGC CTACCACATG 
ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGCGGAACAG CGCCTCGGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCTCTGGG GGACGTGAGC 
AAGAATATGG CCCCGCTGCC AAGCGCACCG CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG 
GCGCAGGTGT ATGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC CGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACACCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAGATGAA GCGCCAAGCC AAGGACAAGG CGGCACAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG CGGGGGCGCA GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG 
CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG GTCCTGGTGA AAGACGGCAA ACCTTGCCAG 
GCGGGCGCCC CCGCGCCGGG CGCCACCAGC CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG 
CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG GCAGCTATTT CAGTGGGCAG CGGTGGCCCC 
GGCCTGGGTG CCCACCCGGG CCACCAGCCG GGCAGCGCGG GCCAGTCTCC GGACCTGGCG 
CACCACGCCG CCAGCCCCGC GGCGCTGCAG GGCCAGGTCT CCAGCCTGCC CCACCTGAAC 
TCCTCGGGCT CGGACTACGG CACCATGTCC TGCTCCACCT TGCTATACGG TCGGACCTGG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa31006
Clone ID Related Accession (Same CDS sequence) XM_005222055.3 , XM_005222055.4
Accession Version XM_005222055.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product homeobox protein Nkx-2.1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222055.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
GGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT TACAGGCAGG GCCAGACGGC ACCGCCGGCC 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCAG TCACCGCCGC CTACCACATG 
ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGCGGAACAG CGCCTCGGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCTCTGGG GGACGTGAGC 
AAGAATATGG CCCCGCTGCC AAGCGCACCG CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG 
GCGCAGGTGT ATGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC CGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACACCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAGATGAA GCGCCAAGCC AAGGACAAGG CGGCACAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG CGGGGGCGCA GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG 
CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG GTCCTGGTGA AAGACGGCAA ACCTTGCCAG 
GCGGGCGCCC CCGCGCCGGG CGCCACCAGC CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG 
CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG GCAGCTATTT CAGTGGGCAG CGGTGGCCCC 
GGCCTGGGTG CCCACCCGGG CCACCAGCCG GGCAGCGCGG GCCAGTCTCC GGACCTGGCG 
CACCACGCCG CCAGCCCCGC GGCGCTGCAG GGCCAGGTCT CCAGCCTGCC CCACCTGAAC 
TCCTCGGGCT CGGACTACGG CACCATGTCC TGCTCCACCT TGCTATACGG TCGGACCTGG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005222112.1
CDS289..1491
Translation

Target ORF information:

RefSeq Version XM_005222055.4
Organism Bos taurus(cattle)
Definition Bos taurus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005222055.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
GGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT TACAGGCAGG GCCAGACGGC ACCGCCGGCC 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCAG TCACCGCCGC CTACCACATG 
ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGCGGAACAG CGCCTCGGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCTCTGGG GGACGTGAGC 
AAGAATATGG CCCCGCTGCC AAGCGCACCG CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG 
GCGCAGGTGT ATGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC CGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACACCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAGATGAA GCGCCAAGCC AAGGACAAGG CGGCACAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG CGGGGGCGCA GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG 
CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG GTCCTGGTGA AAGACGGCAA ACCTTGCCAG 
GCGGGCGCCC CCGCGCCGGG CGCCACCAGC CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG 
CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG GCAGCTATTT CAGTGGGCAG CGGTGGCCCC 
GGCCTGGGTG CCCACCCGGG CCACCAGCCG GGCAGCGCGG GCCAGTCTCC GGACCTGGCG 
CACCACGCCG CCAGCCCCGC GGCGCTGCAG GGCCAGGTCT CCAGCCTGCC CCACCTGAAC 
TCCTCGGGCT CGGACTACGG CACCATGTCC TGCTCCACCT TGCTATACGG TCGGACCTGG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa38723
Clone ID Related Accession (Same CDS sequence) NM_001191458.1 , XM_010817163.2
Accession Version XM_010817163.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product homeobox protein Nkx-2.1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104536.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT 
TACAGGCAGG GCCAGACGGC ACCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCAG TCACCGCCGC CTACCACATG ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGCGGAACAG CGCCTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCTCTGGG GGACGTGAGC AAGAATATGG CCCCGCTGCC AAGCGCACCG 
CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG GCGCAGGTGT ATGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC CGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAAGCC 
AAGGACAAGG CGGCACAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG CGGGGGCGCA 
GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG 
GTCCTGGTGA AAGACGGCAA ACCTTGCCAG GCGGGCGCCC CCGCGCCGGG CGCCACCAGC 
CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG 
GCAGCTATTT CAGTGGGCAG CGGTGGCCCC GGCCTGGGTG CCCACCCGGG CCACCAGCCG 
GGCAGCGCGG GCCAGTCTCC GGACCTGGCG CACCACGCCG CCAGCCCCGC GGCGCTGCAG 
GGCCAGGTCT CCAGCCTGCC CCACCTGAAC TCCTCGGGCT CGGACTACGG CACCATGTCC 
TGCTCCACCT TGCTATACGG TCGGACCTGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010815465.1
CDS92..1204
Translation

Target ORF information:

RefSeq Version XM_010817163.2
Organism Bos taurus(cattle)
Definition Bos taurus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010817163.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCT 
TACAGGCAGG GCCAGACGGC ACCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCAG TCACCGCCGC CTACCACATG ACGGCGGCGG CGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGCGGAACAG CGCCTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCTCTGGG GGACGTGAGC AAGAATATGG CCCCGCTGCC AAGCGCACCG 
CGCCGGAAGC GCCGGGTGCT CTTCTCCCAG GCGCAGGTGT ATGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC CGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAAGCC 
AAGGACAAGG CGGCACAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG CGGGGGCGCA 
GGGTGCCAGC AGCAGCAGCA GCAAGCGCAG CAGCAGTCCC CGCGCCGCGT GGCCGTGCCG 
GTCCTGGTGA AAGACGGCAA ACCTTGCCAG GCGGGCGCCC CCGCGCCGGG CGCCACCAGC 
CTGCAAGGCC ACGCGCAGCA GCAGGCGCAG CAGCAGGCGC AGGCCGCTCA AGCGGCCGCG 
GCAGCTATTT CAGTGGGCAG CGGTGGCCCC GGCCTGGGTG CCCACCCGGG CCACCAGCCG 
GGCAGCGCGG GCCAGTCTCC GGACCTGGCG CACCACGCCG CCAGCCCCGC GGCGCTGCAG 
GGCCAGGTCT CCAGCCTGCC CCACCTGAAC TCCTCGGGCT CGGACTACGG CACCATGTCC 
TGCTCCACCT TGCTATACGG TCGGACCTGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL