MEGF6 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following MEGF6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223968 XM_024976784.1
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223964 XM_005217190.4
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223965 XM_024976782.1
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa223966 XM_024976783.1
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa223967 XM_010813409.3
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa56049 XM_005194844.3
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa56049 XM_005217190.3
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa56050 XM_010813409.2
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa56050 XM_010823129.2
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa92995 XM_015466861.1
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa92995 XM_015475184.1
Latest version!
Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223968
    Clone ID Related Accession (Same CDS sequence) XM_024976784.1
    Accession Version XM_024976784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGGTGAG TCCGGGCCAG CCTGGGGGCG GCGGCGTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832552.1
    CDS31..4413
    Translation

    Target ORF information:

    RefSeq Version XM_024976784.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGGTGAG TCCGGGCCAG CCTGGGGGCG GCGGCGTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223964
    Clone ID Related Accession (Same CDS sequence) XM_005217190.4
    Accession Version XM_005217190.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217190.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217247.3
    CDS29..5071
    Translation

    Target ORF information:

    RefSeq Version XM_005217190.4
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217190.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223965
    Clone ID Related Accession (Same CDS sequence) XM_024976782.1
    Accession Version XM_024976782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832550.1
    CDS30..5024
    Translation

    Target ORF information:

    RefSeq Version XM_024976782.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223966
    Clone ID Related Accession (Same CDS sequence) XM_024976783.1
    Accession Version XM_024976783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGCTGA AGGGCTGCCG TGCCGCCTCG TCGTGGTCAT CCCGCTGGCC CCACGTGTGT 
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832551.1
    CDS46..4788
    Translation

    Target ORF information:

    RefSeq Version XM_024976783.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGCTGA AGGGCTGCCG TGCCGCCTCG TCGTGGTCAT CCCGCTGGCC CCACGTGTGT 
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTCTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTGCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCTG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223967
    Clone ID Related Accession (Same CDS sequence) XM_010813409.3
    Accession Version XM_010813409.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCGC CGTGGAGGCG CGGCGGGCTG GCGGCTCTCT GGTACCTCGG GCTGCTGGGG 
    AGCCTGGCGC GCGTTGCGGG CATGCACTAC CGCTACCTGT GGAGGGGCTG CTACCCGTGC
    CACCTGGGCC AAGCCGGCTA CCCCGTGAGC TCCGGCGACC CGAGGCCAGA CGTGGACGAG
    TGCCAGGTGC ACAATGGCGG CTGCCAGCAC CGGTGTGTGA ACACCCCAGG CTCCTACTTC
    TGCGAGTGCA AGCCCGGCTT CCGTCTCCAC GCGGACGGCA GGACCTGCCT GGCCATCAAC
    TCCTGCGCTG TGGGCAATGG TGGCTGCCAG CACAACTGCG TCCAGCTCAC AGTGACCCAG
    CACCGCTGCC AATGCCGGCC CGAGTTCCAG CTCCAGGAGG ATGGCAGGCG CTGCGTCCGG
    AGAAACCCAT GCGCAGACAG GAATGGCGGC TGCATGCACA GGTGCCGGGC ACTCCGGGGC
    CTTGCCCACT GCGAGTGCCG CGCGGGCTAC CAGCTGGCAG TAGACCGCAA GGCCTGCGAA
    GATGTGGATG AATGTGCCAC GGGCCTGGCC CAGTGTGCAC ACGGCTGCCT CAACACGCAC
    GGCTCCTTCA AGTGTGTGTG CAATGCGGGC TACGAGCTGG GTGCTGATGG CCGGCAGTGC
    TACCGGATCG AGATGGAGAT TGTGAACAGC TGTGAGGCAG ACAACGGCGG CTGCTCCCAC
    GGCTGCAGCC ACAGCAGCGC GGGCCCCGTC TGCACCTGCC CCCATGGCTA CGAGCTGGAT
    GAGGACCAGA GGACGTGTAT CGACGTCGAC GACTGTGCCG CCTCCCCCTG CTGCCAGCAG
    GTTTGCACCA ACAGCCCCGG TGGCTACGAG TGCAGCTGCT ATGCTGGCTA CCGGCTCAGT
    GCGGATGGCT GCGGGTGTGA GGACGTGGAC GAGTGCGCCT CTGGCCGCGG TGGCTGCGAG
    CACCACTGCG CCAACCTGGC CGGCTCCTTC CAGTGCTCCT GTGAGGCCGG CTACCAGCTG
    GACGAGGACC GCAGGGGTTG CACCTCCCTG GAGCTGCCCG AGCTGGTCCT GGACGGCCAC
    CTCCCCCTCG GGCGCCCCCC TCTGCACGTC GCGGTGCTCC AGGACGAGCT GCCCCACCTC
    TTCCAAGACG ACTACGTCGG GGCTGAGGAG GAAGAGGCCG AGGCACGGGG CGAACACACA
    CTGCAGGAAA AGTATGTCTG CCTGGACGAC ACCTTTGGCC CAGACTGCAG CTTGACCTGT
    GATGACTGCA GGAATGGGGG AACCTGCCTC CCCACGTTGG ACAGCTGTGA CTGCCCCGAC
    GGCTGGACCG GGCTCGTCTG CAACGAGACT TGTCCTCCGG ACACCTTCGG GAAGAACTGC
    AGCTTCTCCT GCAGCTGTCA GAACGGCGGG ACCTGTGACC CTGTGACGGG GGCCTGCCGC
    TGCCCCCCAG GGGTCGGCGG AGCCCACTGT GAGGACGGCT GCCCCAAAGG CTTCTATGGC
    AAGCACTGCC GTAAGAAGTG CCACTGTGCC AACCGGGGCC GCTGCCACCG CCTCTACGGG
    GCCTGCCTCT GTGACCCGGG GCTCTACGGC CGCTTCTGCC ACCTGGCCTG CCCGCCATGG
    GCCTTTGGGC CAGGCTGCTC AGAGGAGTGC AAGTGTGAGC AGCAGAATAC GCGTGCGTGC
    GATAAGAGGG ATGGCAGCTG TGCCTGCAAG GCGGGCTTCA GGGGCGAGTG GTGCCAGGAT
    GAGTGCCAGC CGGGCTTCTT TGGGCCAGGG TGCCGGCAGG CGTGCACCTG CCCCCCGGGC
    GTGGCCTGCG ACCCTGTCAG TGGCGAGTGT GGGAAGCAGT GTCCCGCTGG CTACCACGGG
    GAGGACTGCG GCCAAGAGTG CCCAGCAGGG ACGTTTGGCC AGAACTGCTC AGGCTCCTGC
    TCCTGTGGGG ACGCCCCCTG CGACCGGGTC ACAGGGCAAT GTCTGTGCCC CCCGGGGAGG
    ACCGGGGACG ACTGTGGGGC AGATTGTCCC GAGGGCCGCT GGGGGCTTGG CTGCCAGGAG
    ATCTGCCCGC CGTGTGAGCA CGGTGCCGCC TGTGTGCCGG AGACTGGAGC CTGCCTGTGC
    CGCCCCGGCT TCATGGGCAG CCGCTGCCAG GATGCCTGCC CTGCTGGCTG GTTTGGACCT
    GGCTGCCAGA CGCAGTGCTC CTGTGCCAAC GATGGGCTCT GCCACCCAGT GACCGGCCGG
    TGCAGCTGCG CCCCTGGGTG GACTGGCCTC AGCTGCCAGA GAGCCTGTGA CAGCGGCCAC
    TGGGGCCCCG ACTGCAGCTT CACCTGCAAC TGCAGTGCAG GCCACGGGGG CTGTGACGCC
    GTCAGCGGCC TGTGTGTGTG TGAAGCCGGC TACGTGGGCC CGCGGTGTGA GCAGCGGTGT
    CCCCAGGGCT ACTTCGGGCC GGGCTGTGGG CGGCAGTGCC AGTGCGAGCA CGGGGCGGCC
    TGTGACCATG TCAGCGGGGC CTGCACCTGC CCTGCCGGCT GGAGGGGCAC CTTCTGTGAG
    CGAGCCTGCC CAGCCGGCTT CTTTGGAGTG GACTGTGGCC ATGCCTGTGA CTGCAGTGCC
    GGAGTCCCCT GTGATCCTGT GAGCGGCTCC TGCCTCTGTC CTTCCGGCCG CCAGGGCCCC
    CGCTGTGCCC AGACCTGCCC AGCCCACATG TTCGGCCACA ACTGCAGCCA GGCCTGCTCC
    TGCTTTAATG GGGCCTCCTG TGACCCTGTC CACGGGCAGT GCCGCTGTGG CCCTGGCTGG
    ACGGGGCCCA CCTGCCTGCA GGCCTGCCCA GAGGGCCTCT ATGGTGAGGA CTGTCAGCAC
    TCCTGCCTGT GCCAGAATGG GGGCAGCTGT GACCCTGTCT CAGGACACTG CACCTGCCCA
    GAGGGCTGGG CCGGCCTGGC CTGTGAGAAG GAGTGCCTCC CGGGGGTCTT TGAAGTTGGC
    TGCCCGCACA GCTGCGGGTG CCTCAACGGT GGCCTCTGTG ATCCGCACAC CGTCCACTGC
    CTCTGCCCAG CCGGCTGGAC CGGGGACAAG TGCCAGAGCT CCTGTCCCGA GGGCACCTTT
    GGGGTCCTCT GTGAGGAGCG CTGCGCCTGC CGGTGGGGGG CCGCCTGCCA CGCCGTCACT
    GGGGCCTGCC TCTGTGCCCC GGGGTGGAGG GGCTCGCGGT GTGAGAGCGC CTGCCCACCC
    AGCTGGTTTG GAGAGGCCTG TGCCCAGCGC TGCCAGTGCC CCCCGGGCGC CGCCTGCCAC
    CACGTCACCG GGGAGTGTCA CTGTCCCCCG GGCTTCACGG GGCCCAGCTG TGAGCAAGCC
    TGCCCACCTG GCACCTTTGG GGAGCGCTGT GGGCAGCAGT GCCACTGCCC CGGCGAGAAC
    CAGGCCTGCC ACCCCGCCTC AGGGACCTGC GCGTGTGCCC CCGGCTACCA CGGCGCCGGC
    TGCCAGCAAC GGTGCCCACC AGGGCGGTTT GGGCCTGGCT GTGAGCTGCT GTGTGGGTGT
    CTCAACGGGG GCTCTTGTGA CGCGGTCACT GGGGCCTGCC GCTGCCCCGC TGGATTCCTT
    GGGACTGACT GCAGCCTCGC CTGTCCACAC GGCCGCTTTG GCCCTGGCTG TGCCTACGTG
    TGCGGGTGTG GGCAGGGGGC GGCCTGTGAC CCCGTGACCG GCAGCTGCTC CTGTCCGCCT
    GGGAGGACCG GCGTCCACTG CGAGCACGGC TGCCCCCAGA ATCGGTTTGG TGTGAATTGT
    GAGCATGCGT GCTCCTGCAG GAATGGGGGC CTGTGTCACG CCACCAACGG CAGCTGTTCC
    TGTGGCCTGG GCTGGACTGG GCCACACTGC GAGCTGGCCT GCCCCGCCGG GCGCTACGGT
    GCCGCCTGCA GCCTCGAGTG CTCCTGCCAC AACCACGGGG CCTGTGAGCC CACGTCGGGG
    GCCTGCCGCT GCGGCCCCGG CTTCTACGGC CAGGCCTGCG AGCACTCCTG TCCCCCCGGC
    TTCCATGGGG CTGGCTGCCA GCAGACGTGC CAGTGTCAGC ATGGCGCCCC CTGCGACCCT
    GTCAGCGGCC AGTGCCTCTG CCCTGCTGGC CTCCATGGCC AGTTCTGCGA GCGGGGGTGT
    GAGCCGGGCT CGTTCGGAGA GGGCTGCGGC CAGCAGTGTG ACTGCGAGCT TGGGGTGCCC
    TGTGACCCCG TCACCGGCCA CTGCCTGTGC CCCCCTGGGC GCACAGGGAC CACCTGTGAC
    CTGGGCTGCA GGAGGGGCTT CTTCGGGCCG GGCTGTGCCC TGCACTGCTC CTGTGGGGGT
    GGAGCCGACT GCGACCCTGT CAGCGGGCAG TGCCTCTGCG TGGACGGCTA CACAGGGCCC
    ACGTGCCGGC AGGGTGGACC CTCCCAGCTC CCGGAGAGCC CGTCTCCAGC CCTGGGCCCA
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811711.2
    CDS70..4584
    Translation

    Target ORF information:

    RefSeq Version XM_010813409.3
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813409.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCGC CGTGGAGGCG CGGCGGGCTG GCGGCTCTCT GGTACCTCGG GCTGCTGGGG 
    AGCCTGGCGC GCGTTGCGGG CATGCACTAC CGCTACCTGT GGAGGGGCTG CTACCCGTGC
    CACCTGGGCC AAGCCGGCTA CCCCGTGAGC TCCGGCGACC CGAGGCCAGA CGTGGACGAG
    TGCCAGGTGC ACAATGGCGG CTGCCAGCAC CGGTGTGTGA ACACCCCAGG CTCCTACTTC
    TGCGAGTGCA AGCCCGGCTT CCGTCTCCAC GCGGACGGCA GGACCTGCCT GGCCATCAAC
    TCCTGCGCTG TGGGCAATGG TGGCTGCCAG CACAACTGCG TCCAGCTCAC AGTGACCCAG
    CACCGCTGCC AATGCCGGCC CGAGTTCCAG CTCCAGGAGG ATGGCAGGCG CTGCGTCCGG
    AGAAACCCAT GCGCAGACAG GAATGGCGGC TGCATGCACA GGTGCCGGGC ACTCCGGGGC
    CTTGCCCACT GCGAGTGCCG CGCGGGCTAC CAGCTGGCAG TAGACCGCAA GGCCTGCGAA
    GATGTGGATG AATGTGCCAC GGGCCTGGCC CAGTGTGCAC ACGGCTGCCT CAACACGCAC
    GGCTCCTTCA AGTGTGTGTG CAATGCGGGC TACGAGCTGG GTGCTGATGG CCGGCAGTGC
    TACCGGATCG AGATGGAGAT TGTGAACAGC TGTGAGGCAG ACAACGGCGG CTGCTCCCAC
    GGCTGCAGCC ACAGCAGCGC GGGCCCCGTC TGCACCTGCC CCCATGGCTA CGAGCTGGAT
    GAGGACCAGA GGACGTGTAT CGACGTCGAC GACTGTGCCG CCTCCCCCTG CTGCCAGCAG
    GTTTGCACCA ACAGCCCCGG TGGCTACGAG TGCAGCTGCT ATGCTGGCTA CCGGCTCAGT
    GCGGATGGCT GCGGGTGTGA GGACGTGGAC GAGTGCGCCT CTGGCCGCGG TGGCTGCGAG
    CACCACTGCG CCAACCTGGC CGGCTCCTTC CAGTGCTCCT GTGAGGCCGG CTACCAGCTG
    GACGAGGACC GCAGGGGTTG CACCTCCCTG GAGCTGCCCG AGCTGGTCCT GGACGGCCAC
    CTCCCCCTCG GGCGCCCCCC TCTGCACGTC GCGGTGCTCC AGGACGAGCT GCCCCACCTC
    TTCCAAGACG ACTACGTCGG GGCTGAGGAG GAAGAGGCCG AGGCACGGGG CGAACACACA
    CTGCAGGAAA AGTATGTCTG CCTGGACGAC ACCTTTGGCC CAGACTGCAG CTTGACCTGT
    GATGACTGCA GGAATGGGGG AACCTGCCTC CCCACGTTGG ACAGCTGTGA CTGCCCCGAC
    GGCTGGACCG GGCTCGTCTG CAACGAGACT TGTCCTCCGG ACACCTTCGG GAAGAACTGC
    AGCTTCTCCT GCAGCTGTCA GAACGGCGGG ACCTGTGACC CTGTGACGGG GGCCTGCCGC
    TGCCCCCCAG GGGTCGGCGG AGCCCACTGT GAGGACGGCT GCCCCAAAGG CTTCTATGGC
    AAGCACTGCC GTAAGAAGTG CCACTGTGCC AACCGGGGCC GCTGCCACCG CCTCTACGGG
    GCCTGCCTCT GTGACCCGGG GCTCTACGGC CGCTTCTGCC ACCTGGCCTG CCCGCCATGG
    GCCTTTGGGC CAGGCTGCTC AGAGGAGTGC AAGTGTGAGC AGCAGAATAC GCGTGCGTGC
    GATAAGAGGG ATGGCAGCTG TGCCTGCAAG GCGGGCTTCA GGGGCGAGTG GTGCCAGGAT
    GAGTGCCAGC CGGGCTTCTT TGGGCCAGGG TGCCGGCAGG CGTGCACCTG CCCCCCGGGC
    GTGGCCTGCG ACCCTGTCAG TGGCGAGTGT GGGAAGCAGT GTCCCGCTGG CTACCACGGG
    GAGGACTGCG GCCAAGAGTG CCCAGCAGGG ACGTTTGGCC AGAACTGCTC AGGCTCCTGC
    TCCTGTGGGG ACGCCCCCTG CGACCGGGTC ACAGGGCAAT GTCTGTGCCC CCCGGGGAGG
    ACCGGGGACG ACTGTGGGGC AGATTGTCCC GAGGGCCGCT GGGGGCTTGG CTGCCAGGAG
    ATCTGCCCGC CGTGTGAGCA CGGTGCCGCC TGTGTGCCGG AGACTGGAGC CTGCCTGTGC
    CGCCCCGGCT TCATGGGCAG CCGCTGCCAG GATGCCTGCC CTGCTGGCTG GTTTGGACCT
    GGCTGCCAGA CGCAGTGCTC CTGTGCCAAC GATGGGCTCT GCCACCCAGT GACCGGCCGG
    TGCAGCTGCG CCCCTGGGTG GACTGGCCTC AGCTGCCAGA GAGCCTGTGA CAGCGGCCAC
    TGGGGCCCCG ACTGCAGCTT CACCTGCAAC TGCAGTGCAG GCCACGGGGG CTGTGACGCC
    GTCAGCGGCC TGTGTGTGTG TGAAGCCGGC TACGTGGGCC CGCGGTGTGA GCAGCGGTGT
    CCCCAGGGCT ACTTCGGGCC GGGCTGTGGG CGGCAGTGCC AGTGCGAGCA CGGGGCGGCC
    TGTGACCATG TCAGCGGGGC CTGCACCTGC CCTGCCGGCT GGAGGGGCAC CTTCTGTGAG
    CGAGCCTGCC CAGCCGGCTT CTTTGGAGTG GACTGTGGCC ATGCCTGTGA CTGCAGTGCC
    GGAGTCCCCT GTGATCCTGT GAGCGGCTCC TGCCTCTGTC CTTCCGGCCG CCAGGGCCCC
    CGCTGTGCCC AGACCTGCCC AGCCCACATG TTCGGCCACA ACTGCAGCCA GGCCTGCTCC
    TGCTTTAATG GGGCCTCCTG TGACCCTGTC CACGGGCAGT GCCGCTGTGG CCCTGGCTGG
    ACGGGGCCCA CCTGCCTGCA GGCCTGCCCA GAGGGCCTCT ATGGTGAGGA CTGTCAGCAC
    TCCTGCCTGT GCCAGAATGG GGGCAGCTGT GACCCTGTCT CAGGACACTG CACCTGCCCA
    GAGGGCTGGG CCGGCCTGGC CTGTGAGAAG GAGTGCCTCC CGGGGGTCTT TGAAGTTGGC
    TGCCCGCACA GCTGCGGGTG CCTCAACGGT GGCCTCTGTG ATCCGCACAC CGTCCACTGC
    CTCTGCCCAG CCGGCTGGAC CGGGGACAAG TGCCAGAGCT CCTGTCCCGA GGGCACCTTT
    GGGGTCCTCT GTGAGGAGCG CTGCGCCTGC CGGTGGGGGG CCGCCTGCCA CGCCGTCACT
    GGGGCCTGCC TCTGTGCCCC GGGGTGGAGG GGCTCGCGGT GTGAGAGCGC CTGCCCACCC
    AGCTGGTTTG GAGAGGCCTG TGCCCAGCGC TGCCAGTGCC CCCCGGGCGC CGCCTGCCAC
    CACGTCACCG GGGAGTGTCA CTGTCCCCCG GGCTTCACGG GGCCCAGCTG TGAGCAAGCC
    TGCCCACCTG GCACCTTTGG GGAGCGCTGT GGGCAGCAGT GCCACTGCCC CGGCGAGAAC
    CAGGCCTGCC ACCCCGCCTC AGGGACCTGC GCGTGTGCCC CCGGCTACCA CGGCGCCGGC
    TGCCAGCAAC GGTGCCCACC AGGGCGGTTT GGGCCTGGCT GTGAGCTGCT GTGTGGGTGT
    CTCAACGGGG GCTCTTGTGA CGCGGTCACT GGGGCCTGCC GCTGCCCCGC TGGATTCCTT
    GGGACTGACT GCAGCCTCGC CTGTCCACAC GGCCGCTTTG GCCCTGGCTG TGCCTACGTG
    TGCGGGTGTG GGCAGGGGGC GGCCTGTGAC CCCGTGACCG GCAGCTGCTC CTGTCCGCCT
    GGGAGGACCG GCGTCCACTG CGAGCACGGC TGCCCCCAGA ATCGGTTTGG TGTGAATTGT
    GAGCATGCGT GCTCCTGCAG GAATGGGGGC CTGTGTCACG CCACCAACGG CAGCTGTTCC
    TGTGGCCTGG GCTGGACTGG GCCACACTGC GAGCTGGCCT GCCCCGCCGG GCGCTACGGT
    GCCGCCTGCA GCCTCGAGTG CTCCTGCCAC AACCACGGGG CCTGTGAGCC CACGTCGGGG
    GCCTGCCGCT GCGGCCCCGG CTTCTACGGC CAGGCCTGCG AGCACTCCTG TCCCCCCGGC
    TTCCATGGGG CTGGCTGCCA GCAGACGTGC CAGTGTCAGC ATGGCGCCCC CTGCGACCCT
    GTCAGCGGCC AGTGCCTCTG CCCTGCTGGC CTCCATGGCC AGTTCTGCGA GCGGGGGTGT
    GAGCCGGGCT CGTTCGGAGA GGGCTGCGGC CAGCAGTGTG ACTGCGAGCT TGGGGTGCCC
    TGTGACCCCG TCACCGGCCA CTGCCTGTGC CCCCCTGGGC GCACAGGGAC CACCTGTGAC
    CTGGGCTGCA GGAGGGGCTT CTTCGGGCCG GGCTGTGCCC TGCACTGCTC CTGTGGGGGT
    GGAGCCGACT GCGACCCTGT CAGCGGGCAG TGCCTCTGCG TGGACGGCTA CACAGGGCCC
    ACGTGCCGGC AGGGTGGACC CTCCCAGCTC CCGGAGAGCC CGTCTCCAGC CCTGGGCCCA
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa56049
    Clone ID Related Accession (Same CDS sequence) XM_005194844.3 , XM_005217190.3
    Accession Version XM_005194844.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCCG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194901.2
    CDS35..5077
    Translation

    Target ORF information:

    RefSeq Version XM_005194844.3
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005194844.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCCG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa56049
    Clone ID Related Accession (Same CDS sequence) XM_005194844.3 , XM_005217190.3
    Accession Version XM_005217190.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCCG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217247.2
    CDS35..5077
    Translation

    Target ORF information:

    RefSeq Version XM_005217190.3
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217190.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCATTCAG GCGCACAGGA CTCCGCGTTG CCCTCCAGCG CGGGCTCGAA ACCGGGGTCC 
    TGCCCACCTG CGGCGACCTC ACGCTCGGCG GGACCGGGCG CGGCACCTGT GGCTGCCCCC
    GCCGGGTCCC CCGGGCCATG GCGCACGTGG AGGAGGCGGC TACGAGCGCC CGCCTGGGCC
    GCCTGTGAGC GCGGGACCGT AGGAGGGACC CGGCGCGACG CCGCGGGCAC TATGCCTTTC
    CTTCCGGAGG CGGGGGCGGC GGGGCGCGCC GTGGCCCTGG CCCTGGTGCT GCTGCTCCCC
    GCAGTACCCA CGGGCGCCGG CGCCCTGCTC CGCCTGCAAC CGGGCATGCC CCACGTGTGT
    GCGGAGCAGG AGCTGACTCT GGTGGGCCGC CGCCAGCCCT GTGTGCGGGC CTTCAGCCGC
    ACAGTGCCCG TGTGGAGGCC GGGTTGTGGG CGGCAGGCCT GGTGTGTCAG CCACGAGCGC
    AGAACCGTCT ACTACACGGG CTACAGGCAG GTGTACTCCA CAGAGGCCCA AACGGTGTTC
    CGGTGCTGCC CAGGGTGGAG CCAACAGCCA GGGAGCCAGG GCTGCCTCTC CCCCGTGTGC
    AGCTCCGGCC TCTGCTTTAA CGGCGGCCAC TGCGTGTCCG GCTCAGCCCA GCCGTGCCGC
    TGTCCCTCTG GCTTCCAGGG TCCTCGCTGT CAGCATGACG TGGACGAGTG CCAGGTGCAC
    AATGGCGGCT GCCAGCACCG GTGTGTGAAC ACCCCAGGCT CCTACTTCTG CGAGTGCAAG
    CCCGGCTTCC GTCTCCACGC GGACGGCAGG ACCTGCCTGG CCATCAACTC CTGCGCTGTG
    GGCAATGGTG GCTGCCAGCA CAACTGCGTC CAGCTCACAG TGACCCAGCA CCGCTGCCAA
    TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT GGCAGGCGCT GCGTCCGGAG AAACCCATGC
    GCAGACAGGA ATGGCGGCTG CATGCACAGG TGCCGGGCAC TCCGGGGCCT TGCCCACTGC
    GAGTGCCGCG CGGGCTACCA GCTGGCAGTA GACCGCAAGG CCTGCGAAGA TGTGGATGAA
    TGTGCCACGG GCCTGGCCCA GTGTGCACAC GGCTGCCTCA ACACGCACGG CTCCTTCAAG
    TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT GCTGATGGCC GGCAGTGCTA CCGGATCGAG
    ATGGAGATTG TGAACAGCTG TGAGGCAGAC AACGGCGGCT GCTCCCACGG CTGCAGCCAC
    AGCAGCGCGG GCCCCGTCTG CACCTGCCCC CATGGCTACG AGCTGGATGA GGACCAGAGG
    ACGTGTATCG ACGTCGACGA CTGTGCCGCC TCCCCCTGCT GCCAGCAGGT TTGCACCAAC
    AGCCCCGGTG GCTACGAGTG CAGCTGCTAT GCTGGCTACC GGCTCAGTGC GGATGGCTGC
    GGGTGTGAGG ACGTGGACGA GTGCGCCTCT GGCCGCGGTG GCTGCGAGCA CCACTGCGCC
    AACCTGGCCG GCTCCTTCCA GTGCTCCTGT GAGGCCGGCT ACCAGCTGGA CGAGGACCGC
    AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG CTGGTCCTGG ACGGCCACCT CCCCCTCGGG
    CGCCCCCCTC TGCACGTCGC GGTGCTCCAG GACGAGCTGC CCCACCTCTT CCAAGACGAC
    TACGTCGGGG CTGAGGAGGA AGAGGCCGAG GCACGGGGCG AACACACACT GCAGGAAAAG
    TATGTCTGCC TGGACGACAC CTTTGGCCCA GACTGCAGCT TGACCTGTGA TGACTGCAGG
    AATGGGGGAA CCTGCCTCCC CACGTTGGAC AGCTGTGACT GCCCCGACGG CTGGACCGGG
    CTCGTCTGCA ACGAGACTTG TCCTCCGGAC ACCTTCGGGA AGAACTGCAG CTTCTCCTGC
    AGCTGTCAGA ACGGCGGGAC CTGTGACCCT GTGACGGGGG CCTGCCGCTG CCCCCCAGGG
    GTCGGCGGAG CCCACTGTGA GGACGGCTGC CCCAAAGGCT TCTATGGCAA GCACTGCCGT
    AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC TGCCACCGCC TCTACGGGGC CTGCCTCTGT
    GACCCGGGGC TCTACGGCCG CTTCTGCCAC CTGGCCTGCC CGCCATGGGC CTTTGGGCCA
    GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG CAGAATACGC GTGCGTGCGA TAAGAGGGAT
    GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG GGCGAGTGGT GCCAGGATGA GTGCCAGCCG
    GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG TGCACCTGCC CCCCGGGCGT GGCCTGCGAC
    CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT CCCGCTGGCT ACCACGGGGA GGACTGCGGC
    CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG AACTGCTCAG GCTCCTGCTC CTGTGGGGAC
    GCCCCCTGCG ACCGGGTCAC AGGGCAATGT CTGTGCCCCC CGGGGAGGAC CGGGGACGAC
    TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG
    TGTGAGCACG GTGCCGCCTG TGTGCCGGAG ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC
    ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT GCTGGCTGGT TTGGACCTGG CTGCCAGACG
    CAGTGCTCCT GTGCCAACGA TGGGCTCTGC CACCCAGTGA CCGGCCGGTG CAGCTGCGCC
    CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA GCCTGTGACA GCGGCCACTG GGGCCCCGAC
    TGCAGCTTCA CCTGCAACTG CAGTGCAGGC CACGGGGGCT GTGACGCCGT CAGCGGCCTG
    TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC
    TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG TGCGAGCACG GGGCGGCCTG TGACCATGTC
    AGCGGGGCCT GCACCTGCCC TGCCGGCTGG AGGGGCACCT TCTGTGAGCG AGCCTGCCCA
    GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT GCCTGTGACT GCAGTGCCGG AGTCCCCTGT
    GATCCTGTGA GCGGCTCCTG CCTCTGTCCT TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG
    ACCTGCCCAG CCCACATGTT CGGCCACAAC TGCAGCCAGG CCTGCTCCTG CTTTAATGGG
    GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC CGCTGTGGCC CTGGCTGGAC GGGGCCCACC
    TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT GGTGAGGACT GTCAGCACTC CTGCCTGTGC
    CAGAATGGGG GCAGCTGTGA CCCTGTCTCA GGACACTGCA CCTGCCCAGA GGGCTGGGCC
    GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG GGGGTCTTTG AAGTTGGCTG CCCGCACAGC
    TGCGGGTGCC TCAACGGTGG CCTCTGTGAT CCGCACACCG TCCACTGCCT CTGCCCAGCC
    GGCTGGACCG GGGACAAGTG CCAGAGCTCC TGTCCCGAGG GCACCTTTGG GGTCCTCTGT
    GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC GCCTGCCACG CCGTCACTGG GGCCTGCCTC
    TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT GAGAGCGCCT GCCCACCCAG CTGGTTTGGA
    GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC CCGGGCGCCG CCTGCCACCA CGTCACCGGG
    GAGTGTCACT GTCCCCCGGG CTTCACGGGG CCCAGCTGTG AGCAAGCCTG CCCACCTGGC
    ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC CACTGCCCCG GCGAGAACCA GGCCTGCCAC
    CCCGCCTCAG GGACCTGCGC GTGTGCCCCC GGCTACCACG GCGCCGGCTG CCAGCAACGG
    TGCCCACCAG GGCGGTTTGG GCCTGGCTGT GAGCTGCTGT GTGGGTGTCT CAACGGGGGC
    TCTTGTGACG CGGTCACTGG GGCCTGCCGC TGCCCCGCCG GATTCCTTGG GACTGACTGC
    AGCCTCGCCT GTCCACACGG CCGCTTTGGC CCTGGCTGTG CCTACGTGTG CGGGTGTGGG
    CAGGGGGCGG CCTGTGACCC CGTGACCGGC AGCTGCTCCT GTCCGCCTGG GAGGACCGGC
    GTCCACTGCG AGCACGGCTG CCCCCAGAAT CGGTTTGGTG TGAATTGTGA GCATGCGTGC
    TCCTGCAGGA ATGGGGGCCT GTGTCACGCC ACCAACGGCA GCTGTTCCTG TGGCCTGGGC
    TGGACTGGGC CACACTGCGA GCTGGCCTGC CCCGCCGGGC GCTACGGTGC CGCCTGCAGC
    CTCGAGTGCT CCTGCCACAA CCACGGGGCC TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC
    GGCCCCGGCT TCTACGGCCA GGCCTGCGAG CACTCCTGTC CCCCCGGCTT CCATGGGGCT
    GGCTGCCAGC AGACGTGCCA GTGTCAGCAT GGCGCCCCCT GCGACCCTGT CAGCGGCCAG
    TGCCTCTGCC CTGCTGGCCT CCATGGCCAG TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG
    TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC TGCGAGCTTG GGGTGCCCTG TGACCCCGTC
    ACCGGCCACT GCCTGTGCCC CCCTGGGCGC ACAGGGACCA CCTGTGACCT GGGCTGCAGG
    AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG CACTGCTCCT GTGGGGGTGG AGCCGACTGC
    GACCCTGTCA GCGGGCAGTG CCTCTGCGTG GACGGCTACA CAGGGCCCAC GTGCCGGCAG
    GGTGGACCCT CCCAGCTCCC GGAGAGCCCG TCTCCAGCCC TGGGCCCAGC GGCCCTACAA
    CCAGGTTCCC ACCAGACCCG GGCTCAGCAG CAGCCTAGAG AAGCACTAGC TCAGGGGCAG
    CCCCCACTGA GGCTTGGCTC CCAGGGGCCA CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa56050
    Clone ID Related Accession (Same CDS sequence) XM_010813409.2 , XM_010823129.2
    Accession Version XM_010813409.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCGC CGTGGAGGCG CGGCGGGCTG GCGGCTCTCT GGTACCTCGG GCTGCTGGGG 
    AGCCTGGCGC GCGTTGCGGG CATGCACTAC CGCTACCTGT GGAGGGGCTG CTACCCGTGC
    CACCTGGGCC AAGCCGGCTA CCCCGTGAGC TCCGGCGACC CGAGGCCAGA CGTGGACGAG
    TGCCAGGTGC ACAATGGCGG CTGCCAGCAC CGGTGTGTGA ACACCCCAGG CTCCTACTTC
    TGCGAGTGCA AGCCCGGCTT CCGTCTCCAC GCGGACGGCA GGACCTGCCT GGCCATCAAC
    TCCTGCGCTG TGGGCAATGG TGGCTGCCAG CACAACTGCG TCCAGCTCAC AGTGACCCAG
    CACCGCTGCC AATGCCGGCC CGAGTTCCAG CTCCAGGAGG ATGGCAGGCG CTGCGTCCGG
    AGAAACCCAT GCGCAGACAG GAATGGCGGC TGCATGCACA GGTGCCGGGC ACTCCGGGGC
    CTTGCCCACT GCGAGTGCCG CGCGGGCTAC CAGCTGGCAG TAGACCGCAA GGCCTGCGAA
    GATGTGGATG AATGTGCCAC GGGCCTGGCC CAGTGTGCAC ACGGCTGCCT CAACACGCAC
    GGCTCCTTCA AGTGTGTGTG CAATGCGGGC TACGAGCTGG GTGCTGATGG CCGGCAGTGC
    TACCGGATCG AGATGGAGAT TGTGAACAGC TGTGAGGCAG ACAACGGCGG CTGCTCCCAC
    GGCTGCAGCC ACAGCAGCGC GGGCCCCGTC TGCACCTGCC CCCATGGCTA CGAGCTGGAT
    GAGGACCAGA GGACGTGTAT CGACGTCGAC GACTGTGCCG CCTCCCCCTG CTGCCAGCAG
    GTTTGCACCA ACAGCCCCGG TGGCTACGAG TGCAGCTGCT ATGCTGGCTA CCGGCTCAGT
    GCGGATGGCT GCGGGTGTGA GGACGTGGAC GAGTGCGCCT CTGGCCGCGG TGGCTGCGAG
    CACCACTGCG CCAACCTGGC CGGCTCCTTC CAGTGCTCCT GTGAGGCCGG CTACCAGCTG
    GACGAGGACC GCAGGGGTTG CACCTCCCTG GAGCTGCCCG AGCTGGTCCT GGACGGCCAC
    CTCCCCCTCG GGCGCCCCCC TCTGCACGTC GCGGTGCTCC AGGACGAGCT GCCCCACCTC
    TTCCAAGACG ACTACGTCGG GGCTGAGGAG GAAGAGGCCG AGGCACGGGG CGAACACACA
    CTGCAGGAAA AGTATGTCTG CCTGGACGAC ACCTTTGGCC CAGACTGCAG CTTGACCTGT
    GATGACTGCA GGAATGGGGG AACCTGCCTC CCCACGTTGG ACAGCTGTGA CTGCCCCGAC
    GGCTGGACCG GGCTCGTCTG CAACGAGACT TGTCCTCCGG ACACCTTCGG GAAGAACTGC
    AGCTTCTCCT GCAGCTGTCA GAACGGCGGG ACCTGTGACC CTGTGACGGG GGCCTGCCGC
    TGCCCCCCAG GGGTCGGCGG AGCCCACTGT GAGGACGGCT GCCCCAAAGG CTTCTATGGC
    AAGCACTGCC GTAAGAAGTG CCACTGTGCC AACCGGGGCC GCTGCCACCG CCTCTACGGG
    GCCTGCCTCT GTGACCCGGG GCTCTACGGC CGCTTCTGCC ACCTGGCCTG CCCGCCATGG
    GCCTTTGGGC CAGGCTGCTC AGAGGAGTGC AAGTGTGAGC AGCAGAATAC GCGTGCGTGC
    GATAAGAGGG ATGGCAGCTG TGCCTGCAAG GCGGGCTTCA GGGGCGAGTG GTGCCAGGAT
    GAGTGCCAGC CGGGCTTTTT TGGGCCAGGG TGCCGGCAGG CGTGCACCTG CCCCCCGGGC
    GTGGCCTGCG ACCCTGTCAG TGGCGAGTGT GGGAAGCAGT GTCCCGCTGG CTACCACGGG
    GAGGACTGCG GCCAAGAGTG CCCAGCAGGG ACGTTTGGCC AGAACTGCTC AGGCTCCTGC
    TCCTGTGGGG ACGCCCCCTG CGACCGGGTC ACAGGGCAAT GTCTGTGCCC CCCGGGGAGG
    ACCGGGGACG ACTGTGGGGC AGATTGTCCC GAGGGCCGCT GGGGGCTTGG CTGCCAGGAG
    ATCTGCCCGC CGTGTGAGCA CGGTGCCGCC TGTGTGCCGG AGACTGGAGC CTGCCTGTGC
    CGCCCCGGCT TCATGGGCAG CCGCTGCCAG GATGCCTGCC CTGCTGGCTG GTTTGGACCT
    GGCTGCCAGA CGCAGTGCTC CTGTGCCAAC GATGGGCTCT GCCACCCAGT GACCGGCCGG
    TGCAGCTGCG CCCCTGGGTG GACTGGCCTC AGCTGCCAGA GAGCCTGTGA CAGCGGCCAC
    TGGGGCCCCG ACTGCAGCTT CACCTGCAAC TGCAGTGCAG GCCACGGGGG CTGTGACGCC
    GTCAGCGGCC TGTGTGTGTG TGAAGCCGGC TACGTGGGCC CGCGGTGTGA GCAGCGGTGT
    CCCCAGGGCT ACTTCGGGCC GGGCTGTGGG CGGCAGTGCC AGTGCGAGCA CGGGGCGGCC
    TGTGACCATG TCAGCGGGGC CTGCACCTGC CCTGCCGGCT GGAGGGGCAC CTTCTGTGAG
    CGAGCCTGCC CAGCCGGCTT CTTTGGAGTG GACTGTGGCC ATGCCTGTGA CTGCAGTGCC
    GGAGTCCCCT GTGATCCTGT GAGCGGCTCC TGCCTCTGTC CTTCCGGCCG CCAGGGCCCC
    CGCTGTGCCC AGACCTGCCC AGCCCACATG TTCGGCCACA ACTGCAGCCA GGCCTGCTCC
    TGCTTTAATG GGGCCTCCTG TGACCCTGTC CACGGGCAGT GCCGCTGTGG CCCTGGCTGG
    ACGGGGCCCA CCTGCCTGCA GGCCTGCCCA GAGGGCCTCT ATGGTGAGGA CTGTCAGCAC
    TCCTGCCTGT GCCAGAATGG GGGCAGCTGT GACCCTGTCT CAGGACACTG CACCTGCCCA
    GAGGGCTGGG CCGGCCTGGC CTGTGAGAAG GAGTGCCTCC CGGGGGTCTT TGAAGTTGGC
    TGCCCGCACA GCTGCGGGTG CCTCAACGGT GGCCTCTGTG ATCCGCACAC CGTCCACTGC
    CTCTGCCCAG CCGGCTGGAC CGGGGACAAG TGCCAGAGCT CCTGTCCCGA GGGCACCTTT
    GGGGTCCTCT GTGAGGAGCG CTGCGCCTGC CGGTGGGGGG CCGCCTGCCA CGCCGTCACT
    GGGGCCTGCC TCTGTCCCCC GGGGTGGAGG GGCTCGCGGT GTGAGAGCGC CTGCCCACCC
    AGCTGGTTTG GAGAGGCCTG TGCCCAGCGC TGCCAGTGCC CCCCGGGCGC CGCCTGCCAC
    CACGTCACCG GGGAGTGTCA CTGTCCCCCG GGCTTCACGG GGCCCAGCTG TGAGCAAGCC
    TGCCCACCTG GCACCTTTGG GGAGCGCTGT GGGCAGCAGT GCCACTGCCC CGGCGAGAAC
    CAGGCCTGCC ACCCCGCCTC AGGGACCTGC GCGTGTGCCC CCGGCTACCA CGGCGCCGGC
    TGCCAGCAAC GGTGCCCACC AGGGCGGTTT GGGCCTGGCT GTGAGCTGCT GTGTGGGTGT
    CTCAACGGGG GCTCTTGTGA CGCGGTCACT GGGGCCTGCC GCTGCCCCGC CGGATTCCTT
    GGGACTGACT GCAGCCTCGC CTGTCCACAC GGCCGCTTTG GCCCTGGCTG TGCCTACGTG
    TGCGGGTGTG GGCAGGGGGC GGCCTGTGAC CCCGTGACCG GCAGCTGCTC CTGTCCGCCT
    GGGAGGACCG GCGTCCACTG CGAGCACGGC TGCCCCCAGA ATCGGTTTGG TGTGAATTGT
    GAGCATGCGT GCTCCTGCAG GAATGGGGGC CTGTGTCACG CCACCAACGG CAGCTGTTCC
    TGTGGCCTGG GCTGGACTGG GCCACACTGC GAGCTGGCCT GCCCCGCCGG GCGCTACGGT
    GCCGCCTGCA GCCTCGAGTG CTCCTGCCAC AACCACGGGG CCTGTGAGCC CACGTCGGGG
    GCCTGCCGCT GCGGCCCCGG CTTCTACGGC CAGGCCTGCG AGCACTCCTG TCCCCCCGGC
    TTCCATGGGG CTGGCTGCCA GCAGACGTGC CAGTGTCAGC ATGGCGCCCC CTGCGACCCT
    GTCAGCGGCC AGTGCCTCTG CCCTGCTGGC CTCCATGGCC AGTTCTGCGA GCGGGGGTGT
    GAGCCGGGCT CGTTCGGAGA GGGCTGCGGC CAGCAGTGTG ACTGCGAGCT TGGGGTGCCC
    TGTGACCCCG TCACCGGCCA CTGCCTGTGC CCCCCTGGGC GCACAGGGAC CACCTGTGAC
    CTGGGCTGCA GGAGGGGCTT CTTCGGGCCG GGCTGTGCCC TGCACTGCTC CTGTGGGGGT
    GGAGCCGACT GCGACCCTGT CAGCGGGCAG TGCCTCTGCG TGGACGGCTA CACAGGGCCC
    ACGTGCCGGC AGGGTGGACC CTCCCAGCTC CCGGAGAGCC CGTCTCCAGC CCTGGGCCCA
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811711.1
    CDS86..4600
    Translation

    Target ORF information:

    RefSeq Version XM_010813409.2
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813409.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCGC CGTGGAGGCG CGGCGGGCTG GCGGCTCTCT GGTACCTCGG GCTGCTGGGG 
    AGCCTGGCGC GCGTTGCGGG CATGCACTAC CGCTACCTGT GGAGGGGCTG CTACCCGTGC
    CACCTGGGCC AAGCCGGCTA CCCCGTGAGC TCCGGCGACC CGAGGCCAGA CGTGGACGAG
    TGCCAGGTGC ACAATGGCGG CTGCCAGCAC CGGTGTGTGA ACACCCCAGG CTCCTACTTC
    TGCGAGTGCA AGCCCGGCTT CCGTCTCCAC GCGGACGGCA GGACCTGCCT GGCCATCAAC
    TCCTGCGCTG TGGGCAATGG TGGCTGCCAG CACAACTGCG TCCAGCTCAC AGTGACCCAG
    CACCGCTGCC AATGCCGGCC CGAGTTCCAG CTCCAGGAGG ATGGCAGGCG CTGCGTCCGG
    AGAAACCCAT GCGCAGACAG GAATGGCGGC TGCATGCACA GGTGCCGGGC ACTCCGGGGC
    CTTGCCCACT GCGAGTGCCG CGCGGGCTAC CAGCTGGCAG TAGACCGCAA GGCCTGCGAA
    GATGTGGATG AATGTGCCAC GGGCCTGGCC CAGTGTGCAC ACGGCTGCCT CAACACGCAC
    GGCTCCTTCA AGTGTGTGTG CAATGCGGGC TACGAGCTGG GTGCTGATGG CCGGCAGTGC
    TACCGGATCG AGATGGAGAT TGTGAACAGC TGTGAGGCAG ACAACGGCGG CTGCTCCCAC
    GGCTGCAGCC ACAGCAGCGC GGGCCCCGTC TGCACCTGCC CCCATGGCTA CGAGCTGGAT
    GAGGACCAGA GGACGTGTAT CGACGTCGAC GACTGTGCCG CCTCCCCCTG CTGCCAGCAG
    GTTTGCACCA ACAGCCCCGG TGGCTACGAG TGCAGCTGCT ATGCTGGCTA CCGGCTCAGT
    GCGGATGGCT GCGGGTGTGA GGACGTGGAC GAGTGCGCCT CTGGCCGCGG TGGCTGCGAG
    CACCACTGCG CCAACCTGGC CGGCTCCTTC CAGTGCTCCT GTGAGGCCGG CTACCAGCTG
    GACGAGGACC GCAGGGGTTG CACCTCCCTG GAGCTGCCCG AGCTGGTCCT GGACGGCCAC
    CTCCCCCTCG GGCGCCCCCC TCTGCACGTC GCGGTGCTCC AGGACGAGCT GCCCCACCTC
    TTCCAAGACG ACTACGTCGG GGCTGAGGAG GAAGAGGCCG AGGCACGGGG CGAACACACA
    CTGCAGGAAA AGTATGTCTG CCTGGACGAC ACCTTTGGCC CAGACTGCAG CTTGACCTGT
    GATGACTGCA GGAATGGGGG AACCTGCCTC CCCACGTTGG ACAGCTGTGA CTGCCCCGAC
    GGCTGGACCG GGCTCGTCTG CAACGAGACT TGTCCTCCGG ACACCTTCGG GAAGAACTGC
    AGCTTCTCCT GCAGCTGTCA GAACGGCGGG ACCTGTGACC CTGTGACGGG GGCCTGCCGC
    TGCCCCCCAG GGGTCGGCGG AGCCCACTGT GAGGACGGCT GCCCCAAAGG CTTCTATGGC
    AAGCACTGCC GTAAGAAGTG CCACTGTGCC AACCGGGGCC GCTGCCACCG CCTCTACGGG
    GCCTGCCTCT GTGACCCGGG GCTCTACGGC CGCTTCTGCC ACCTGGCCTG CCCGCCATGG
    GCCTTTGGGC CAGGCTGCTC AGAGGAGTGC AAGTGTGAGC AGCAGAATAC GCGTGCGTGC
    GATAAGAGGG ATGGCAGCTG TGCCTGCAAG GCGGGCTTCA GGGGCGAGTG GTGCCAGGAT
    GAGTGCCAGC CGGGCTTTTT TGGGCCAGGG TGCCGGCAGG CGTGCACCTG CCCCCCGGGC
    GTGGCCTGCG ACCCTGTCAG TGGCGAGTGT GGGAAGCAGT GTCCCGCTGG CTACCACGGG
    GAGGACTGCG GCCAAGAGTG CCCAGCAGGG ACGTTTGGCC AGAACTGCTC AGGCTCCTGC
    TCCTGTGGGG ACGCCCCCTG CGACCGGGTC ACAGGGCAAT GTCTGTGCCC CCCGGGGAGG
    ACCGGGGACG ACTGTGGGGC AGATTGTCCC GAGGGCCGCT GGGGGCTTGG CTGCCAGGAG
    ATCTGCCCGC CGTGTGAGCA CGGTGCCGCC TGTGTGCCGG AGACTGGAGC CTGCCTGTGC
    CGCCCCGGCT TCATGGGCAG CCGCTGCCAG GATGCCTGCC CTGCTGGCTG GTTTGGACCT
    GGCTGCCAGA CGCAGTGCTC CTGTGCCAAC GATGGGCTCT GCCACCCAGT GACCGGCCGG
    TGCAGCTGCG CCCCTGGGTG GACTGGCCTC AGCTGCCAGA GAGCCTGTGA CAGCGGCCAC
    TGGGGCCCCG ACTGCAGCTT CACCTGCAAC TGCAGTGCAG GCCACGGGGG CTGTGACGCC
    GTCAGCGGCC TGTGTGTGTG TGAAGCCGGC TACGTGGGCC CGCGGTGTGA GCAGCGGTGT
    CCCCAGGGCT ACTTCGGGCC GGGCTGTGGG CGGCAGTGCC AGTGCGAGCA CGGGGCGGCC
    TGTGACCATG TCAGCGGGGC CTGCACCTGC CCTGCCGGCT GGAGGGGCAC CTTCTGTGAG
    CGAGCCTGCC CAGCCGGCTT CTTTGGAGTG GACTGTGGCC ATGCCTGTGA CTGCAGTGCC
    GGAGTCCCCT GTGATCCTGT GAGCGGCTCC TGCCTCTGTC CTTCCGGCCG CCAGGGCCCC
    CGCTGTGCCC AGACCTGCCC AGCCCACATG TTCGGCCACA ACTGCAGCCA GGCCTGCTCC
    TGCTTTAATG GGGCCTCCTG TGACCCTGTC CACGGGCAGT GCCGCTGTGG CCCTGGCTGG
    ACGGGGCCCA CCTGCCTGCA GGCCTGCCCA GAGGGCCTCT ATGGTGAGGA CTGTCAGCAC
    TCCTGCCTGT GCCAGAATGG GGGCAGCTGT GACCCTGTCT CAGGACACTG CACCTGCCCA
    GAGGGCTGGG CCGGCCTGGC CTGTGAGAAG GAGTGCCTCC CGGGGGTCTT TGAAGTTGGC
    TGCCCGCACA GCTGCGGGTG CCTCAACGGT GGCCTCTGTG ATCCGCACAC CGTCCACTGC
    CTCTGCCCAG CCGGCTGGAC CGGGGACAAG TGCCAGAGCT CCTGTCCCGA GGGCACCTTT
    GGGGTCCTCT GTGAGGAGCG CTGCGCCTGC CGGTGGGGGG CCGCCTGCCA CGCCGTCACT
    GGGGCCTGCC TCTGTCCCCC GGGGTGGAGG GGCTCGCGGT GTGAGAGCGC CTGCCCACCC
    AGCTGGTTTG GAGAGGCCTG TGCCCAGCGC TGCCAGTGCC CCCCGGGCGC CGCCTGCCAC
    CACGTCACCG GGGAGTGTCA CTGTCCCCCG GGCTTCACGG GGCCCAGCTG TGAGCAAGCC
    TGCCCACCTG GCACCTTTGG GGAGCGCTGT GGGCAGCAGT GCCACTGCCC CGGCGAGAAC
    CAGGCCTGCC ACCCCGCCTC AGGGACCTGC GCGTGTGCCC CCGGCTACCA CGGCGCCGGC
    TGCCAGCAAC GGTGCCCACC AGGGCGGTTT GGGCCTGGCT GTGAGCTGCT GTGTGGGTGT
    CTCAACGGGG GCTCTTGTGA CGCGGTCACT GGGGCCTGCC GCTGCCCCGC CGGATTCCTT
    GGGACTGACT GCAGCCTCGC CTGTCCACAC GGCCGCTTTG GCCCTGGCTG TGCCTACGTG
    TGCGGGTGTG GGCAGGGGGC GGCCTGTGAC CCCGTGACCG GCAGCTGCTC CTGTCCGCCT
    GGGAGGACCG GCGTCCACTG CGAGCACGGC TGCCCCCAGA ATCGGTTTGG TGTGAATTGT
    GAGCATGCGT GCTCCTGCAG GAATGGGGGC CTGTGTCACG CCACCAACGG CAGCTGTTCC
    TGTGGCCTGG GCTGGACTGG GCCACACTGC GAGCTGGCCT GCCCCGCCGG GCGCTACGGT
    GCCGCCTGCA GCCTCGAGTG CTCCTGCCAC AACCACGGGG CCTGTGAGCC CACGTCGGGG
    GCCTGCCGCT GCGGCCCCGG CTTCTACGGC CAGGCCTGCG AGCACTCCTG TCCCCCCGGC
    TTCCATGGGG CTGGCTGCCA GCAGACGTGC CAGTGTCAGC ATGGCGCCCC CTGCGACCCT
    GTCAGCGGCC AGTGCCTCTG CCCTGCTGGC CTCCATGGCC AGTTCTGCGA GCGGGGGTGT
    GAGCCGGGCT CGTTCGGAGA GGGCTGCGGC CAGCAGTGTG ACTGCGAGCT TGGGGTGCCC
    TGTGACCCCG TCACCGGCCA CTGCCTGTGC CCCCCTGGGC GCACAGGGAC CACCTGTGAC
    CTGGGCTGCA GGAGGGGCTT CTTCGGGCCG GGCTGTGCCC TGCACTGCTC CTGTGGGGGT
    GGAGCCGACT GCGACCCTGT CAGCGGGCAG TGCCTCTGCG TGGACGGCTA CACAGGGCCC
    ACGTGCCGGC AGGGTGGACC CTCCCAGCTC CCGGAGAGCC CGTCTCCAGC CCTGGGCCCA
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa56050
    Clone ID Related Accession (Same CDS sequence) XM_010813409.2 , XM_010823129.2
    Accession Version XM_010823129.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCGC CGTGGAGGCG CGGCGGGCTG GCGGCTCTCT GGTACCTCGG GCTGCTGGGG 
    AGCCTGGCGC GCGTTGCGGG CATGCACTAC CGCTACCTGT GGAGGGGCTG CTACCCGTGC
    CACCTGGGCC AAGCCGGCTA CCCCGTGAGC TCCGGCGACC CGAGGCCAGA CGTGGACGAG
    TGCCAGGTGC ACAATGGCGG CTGCCAGCAC CGGTGTGTGA ACACCCCAGG CTCCTACTTC
    TGCGAGTGCA AGCCCGGCTT CCGTCTCCAC GCGGACGGCA GGACCTGCCT GGCCATCAAC
    TCCTGCGCTG TGGGCAATGG TGGCTGCCAG CACAACTGCG TCCAGCTCAC AGTGACCCAG
    CACCGCTGCC AATGCCGGCC CGAGTTCCAG CTCCAGGAGG ATGGCAGGCG CTGCGTCCGG
    AGAAACCCAT GCGCAGACAG GAATGGCGGC TGCATGCACA GGTGCCGGGC ACTCCGGGGC
    CTTGCCCACT GCGAGTGCCG CGCGGGCTAC CAGCTGGCAG TAGACCGCAA GGCCTGCGAA
    GATGTGGATG AATGTGCCAC GGGCCTGGCC CAGTGTGCAC ACGGCTGCCT CAACACGCAC
    GGCTCCTTCA AGTGTGTGTG CAATGCGGGC TACGAGCTGG GTGCTGATGG CCGGCAGTGC
    TACCGGATCG AGATGGAGAT TGTGAACAGC TGTGAGGCAG ACAACGGCGG CTGCTCCCAC
    GGCTGCAGCC ACAGCAGCGC GGGCCCCGTC TGCACCTGCC CCCATGGCTA CGAGCTGGAT
    GAGGACCAGA GGACGTGTAT CGACGTCGAC GACTGTGCCG CCTCCCCCTG CTGCCAGCAG
    GTTTGCACCA ACAGCCCCGG TGGCTACGAG TGCAGCTGCT ATGCTGGCTA CCGGCTCAGT
    GCGGATGGCT GCGGGTGTGA GGACGTGGAC GAGTGCGCCT CTGGCCGCGG TGGCTGCGAG
    CACCACTGCG CCAACCTGGC CGGCTCCTTC CAGTGCTCCT GTGAGGCCGG CTACCAGCTG
    GACGAGGACC GCAGGGGTTG CACCTCCCTG GAGCTGCCCG AGCTGGTCCT GGACGGCCAC
    CTCCCCCTCG GGCGCCCCCC TCTGCACGTC GCGGTGCTCC AGGACGAGCT GCCCCACCTC
    TTCCAAGACG ACTACGTCGG GGCTGAGGAG GAAGAGGCCG AGGCACGGGG CGAACACACA
    CTGCAGGAAA AGTATGTCTG CCTGGACGAC ACCTTTGGCC CAGACTGCAG CTTGACCTGT
    GATGACTGCA GGAATGGGGG AACCTGCCTC CCCACGTTGG ACAGCTGTGA CTGCCCCGAC
    GGCTGGACCG GGCTCGTCTG CAACGAGACT TGTCCTCCGG ACACCTTCGG GAAGAACTGC
    AGCTTCTCCT GCAGCTGTCA GAACGGCGGG ACCTGTGACC CTGTGACGGG GGCCTGCCGC
    TGCCCCCCAG GGGTCGGCGG AGCCCACTGT GAGGACGGCT GCCCCAAAGG CTTCTATGGC
    AAGCACTGCC GTAAGAAGTG CCACTGTGCC AACCGGGGCC GCTGCCACCG CCTCTACGGG
    GCCTGCCTCT GTGACCCGGG GCTCTACGGC CGCTTCTGCC ACCTGGCCTG CCCGCCATGG
    GCCTTTGGGC CAGGCTGCTC AGAGGAGTGC AAGTGTGAGC AGCAGAATAC GCGTGCGTGC
    GATAAGAGGG ATGGCAGCTG TGCCTGCAAG GCGGGCTTCA GGGGCGAGTG GTGCCAGGAT
    GAGTGCCAGC CGGGCTTTTT TGGGCCAGGG TGCCGGCAGG CGTGCACCTG CCCCCCGGGC
    GTGGCCTGCG ACCCTGTCAG TGGCGAGTGT GGGAAGCAGT GTCCCGCTGG CTACCACGGG
    GAGGACTGCG GCCAAGAGTG CCCAGCAGGG ACGTTTGGCC AGAACTGCTC AGGCTCCTGC
    TCCTGTGGGG ACGCCCCCTG CGACCGGGTC ACAGGGCAAT GTCTGTGCCC CCCGGGGAGG
    ACCGGGGACG ACTGTGGGGC AGATTGTCCC GAGGGCCGCT GGGGGCTTGG CTGCCAGGAG
    ATCTGCCCGC CGTGTGAGCA CGGTGCCGCC TGTGTGCCGG AGACTGGAGC CTGCCTGTGC
    CGCCCCGGCT TCATGGGCAG CCGCTGCCAG GATGCCTGCC CTGCTGGCTG GTTTGGACCT
    GGCTGCCAGA CGCAGTGCTC CTGTGCCAAC GATGGGCTCT GCCACCCAGT GACCGGCCGG
    TGCAGCTGCG CCCCTGGGTG GACTGGCCTC AGCTGCCAGA GAGCCTGTGA CAGCGGCCAC
    TGGGGCCCCG ACTGCAGCTT CACCTGCAAC TGCAGTGCAG GCCACGGGGG CTGTGACGCC
    GTCAGCGGCC TGTGTGTGTG TGAAGCCGGC TACGTGGGCC CGCGGTGTGA GCAGCGGTGT
    CCCCAGGGCT ACTTCGGGCC GGGCTGTGGG CGGCAGTGCC AGTGCGAGCA CGGGGCGGCC
    TGTGACCATG TCAGCGGGGC CTGCACCTGC CCTGCCGGCT GGAGGGGCAC CTTCTGTGAG
    CGAGCCTGCC CAGCCGGCTT CTTTGGAGTG GACTGTGGCC ATGCCTGTGA CTGCAGTGCC
    GGAGTCCCCT GTGATCCTGT GAGCGGCTCC TGCCTCTGTC CTTCCGGCCG CCAGGGCCCC
    CGCTGTGCCC AGACCTGCCC AGCCCACATG TTCGGCCACA ACTGCAGCCA GGCCTGCTCC
    TGCTTTAATG GGGCCTCCTG TGACCCTGTC CACGGGCAGT GCCGCTGTGG CCCTGGCTGG
    ACGGGGCCCA CCTGCCTGCA GGCCTGCCCA GAGGGCCTCT ATGGTGAGGA CTGTCAGCAC
    TCCTGCCTGT GCCAGAATGG GGGCAGCTGT GACCCTGTCT CAGGACACTG CACCTGCCCA
    GAGGGCTGGG CCGGCCTGGC CTGTGAGAAG GAGTGCCTCC CGGGGGTCTT TGAAGTTGGC
    TGCCCGCACA GCTGCGGGTG CCTCAACGGT GGCCTCTGTG ATCCGCACAC CGTCCACTGC
    CTCTGCCCAG CCGGCTGGAC CGGGGACAAG TGCCAGAGCT CCTGTCCCGA GGGCACCTTT
    GGGGTCCTCT GTGAGGAGCG CTGCGCCTGC CGGTGGGGGG CCGCCTGCCA CGCCGTCACT
    GGGGCCTGCC TCTGTCCCCC GGGGTGGAGG GGCTCGCGGT GTGAGAGCGC CTGCCCACCC
    AGCTGGTTTG GAGAGGCCTG TGCCCAGCGC TGCCAGTGCC CCCCGGGCGC CGCCTGCCAC
    CACGTCACCG GGGAGTGTCA CTGTCCCCCG GGCTTCACGG GGCCCAGCTG TGAGCAAGCC
    TGCCCACCTG GCACCTTTGG GGAGCGCTGT GGGCAGCAGT GCCACTGCCC CGGCGAGAAC
    CAGGCCTGCC ACCCCGCCTC AGGGACCTGC GCGTGTGCCC CCGGCTACCA CGGCGCCGGC
    TGCCAGCAAC GGTGCCCACC AGGGCGGTTT GGGCCTGGCT GTGAGCTGCT GTGTGGGTGT
    CTCAACGGGG GCTCTTGTGA CGCGGTCACT GGGGCCTGCC GCTGCCCCGC CGGATTCCTT
    GGGACTGACT GCAGCCTCGC CTGTCCACAC GGCCGCTTTG GCCCTGGCTG TGCCTACGTG
    TGCGGGTGTG GGCAGGGGGC GGCCTGTGAC CCCGTGACCG GCAGCTGCTC CTGTCCGCCT
    GGGAGGACCG GCGTCCACTG CGAGCACGGC TGCCCCCAGA ATCGGTTTGG TGTGAATTGT
    GAGCATGCGT GCTCCTGCAG GAATGGGGGC CTGTGTCACG CCACCAACGG CAGCTGTTCC
    TGTGGCCTGG GCTGGACTGG GCCACACTGC GAGCTGGCCT GCCCCGCCGG GCGCTACGGT
    GCCGCCTGCA GCCTCGAGTG CTCCTGCCAC AACCACGGGG CCTGTGAGCC CACGTCGGGG
    GCCTGCCGCT GCGGCCCCGG CTTCTACGGC CAGGCCTGCG AGCACTCCTG TCCCCCCGGC
    TTCCATGGGG CTGGCTGCCA GCAGACGTGC CAGTGTCAGC ATGGCGCCCC CTGCGACCCT
    GTCAGCGGCC AGTGCCTCTG CCCTGCTGGC CTCCATGGCC AGTTCTGCGA GCGGGGGTGT
    GAGCCGGGCT CGTTCGGAGA GGGCTGCGGC CAGCAGTGTG ACTGCGAGCT TGGGGTGCCC
    TGTGACCCCG TCACCGGCCA CTGCCTGTGC CCCCCTGGGC GCACAGGGAC CACCTGTGAC
    CTGGGCTGCA GGAGGGGCTT CTTCGGGCCG GGCTGTGCCC TGCACTGCTC CTGTGGGGGT
    GGAGCCGACT GCGACCCTGT CAGCGGGCAG TGCCTCTGCG TGGACGGCTA CACAGGGCCC
    ACGTGCCGGC AGGGTGGACC CTCCCAGCTC CCGGAGAGCC CGTCTCCAGC CCTGGGCCCA
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821431.1
    CDS86..4600
    Translation

    Target ORF information:

    RefSeq Version XM_010823129.2
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823129.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCGC CGTGGAGGCG CGGCGGGCTG GCGGCTCTCT GGTACCTCGG GCTGCTGGGG 
    AGCCTGGCGC GCGTTGCGGG CATGCACTAC CGCTACCTGT GGAGGGGCTG CTACCCGTGC
    CACCTGGGCC AAGCCGGCTA CCCCGTGAGC TCCGGCGACC CGAGGCCAGA CGTGGACGAG
    TGCCAGGTGC ACAATGGCGG CTGCCAGCAC CGGTGTGTGA ACACCCCAGG CTCCTACTTC
    TGCGAGTGCA AGCCCGGCTT CCGTCTCCAC GCGGACGGCA GGACCTGCCT GGCCATCAAC
    TCCTGCGCTG TGGGCAATGG TGGCTGCCAG CACAACTGCG TCCAGCTCAC AGTGACCCAG
    CACCGCTGCC AATGCCGGCC CGAGTTCCAG CTCCAGGAGG ATGGCAGGCG CTGCGTCCGG
    AGAAACCCAT GCGCAGACAG GAATGGCGGC TGCATGCACA GGTGCCGGGC ACTCCGGGGC
    CTTGCCCACT GCGAGTGCCG CGCGGGCTAC CAGCTGGCAG TAGACCGCAA GGCCTGCGAA
    GATGTGGATG AATGTGCCAC GGGCCTGGCC CAGTGTGCAC ACGGCTGCCT CAACACGCAC
    GGCTCCTTCA AGTGTGTGTG CAATGCGGGC TACGAGCTGG GTGCTGATGG CCGGCAGTGC
    TACCGGATCG AGATGGAGAT TGTGAACAGC TGTGAGGCAG ACAACGGCGG CTGCTCCCAC
    GGCTGCAGCC ACAGCAGCGC GGGCCCCGTC TGCACCTGCC CCCATGGCTA CGAGCTGGAT
    GAGGACCAGA GGACGTGTAT CGACGTCGAC GACTGTGCCG CCTCCCCCTG CTGCCAGCAG
    GTTTGCACCA ACAGCCCCGG TGGCTACGAG TGCAGCTGCT ATGCTGGCTA CCGGCTCAGT
    GCGGATGGCT GCGGGTGTGA GGACGTGGAC GAGTGCGCCT CTGGCCGCGG TGGCTGCGAG
    CACCACTGCG CCAACCTGGC CGGCTCCTTC CAGTGCTCCT GTGAGGCCGG CTACCAGCTG
    GACGAGGACC GCAGGGGTTG CACCTCCCTG GAGCTGCCCG AGCTGGTCCT GGACGGCCAC
    CTCCCCCTCG GGCGCCCCCC TCTGCACGTC GCGGTGCTCC AGGACGAGCT GCCCCACCTC
    TTCCAAGACG ACTACGTCGG GGCTGAGGAG GAAGAGGCCG AGGCACGGGG CGAACACACA
    CTGCAGGAAA AGTATGTCTG CCTGGACGAC ACCTTTGGCC CAGACTGCAG CTTGACCTGT
    GATGACTGCA GGAATGGGGG AACCTGCCTC CCCACGTTGG ACAGCTGTGA CTGCCCCGAC
    GGCTGGACCG GGCTCGTCTG CAACGAGACT TGTCCTCCGG ACACCTTCGG GAAGAACTGC
    AGCTTCTCCT GCAGCTGTCA GAACGGCGGG ACCTGTGACC CTGTGACGGG GGCCTGCCGC
    TGCCCCCCAG GGGTCGGCGG AGCCCACTGT GAGGACGGCT GCCCCAAAGG CTTCTATGGC
    AAGCACTGCC GTAAGAAGTG CCACTGTGCC AACCGGGGCC GCTGCCACCG CCTCTACGGG
    GCCTGCCTCT GTGACCCGGG GCTCTACGGC CGCTTCTGCC ACCTGGCCTG CCCGCCATGG
    GCCTTTGGGC CAGGCTGCTC AGAGGAGTGC AAGTGTGAGC AGCAGAATAC GCGTGCGTGC
    GATAAGAGGG ATGGCAGCTG TGCCTGCAAG GCGGGCTTCA GGGGCGAGTG GTGCCAGGAT
    GAGTGCCAGC CGGGCTTTTT TGGGCCAGGG TGCCGGCAGG CGTGCACCTG CCCCCCGGGC
    GTGGCCTGCG ACCCTGTCAG TGGCGAGTGT GGGAAGCAGT GTCCCGCTGG CTACCACGGG
    GAGGACTGCG GCCAAGAGTG CCCAGCAGGG ACGTTTGGCC AGAACTGCTC AGGCTCCTGC
    TCCTGTGGGG ACGCCCCCTG CGACCGGGTC ACAGGGCAAT GTCTGTGCCC CCCGGGGAGG
    ACCGGGGACG ACTGTGGGGC AGATTGTCCC GAGGGCCGCT GGGGGCTTGG CTGCCAGGAG
    ATCTGCCCGC CGTGTGAGCA CGGTGCCGCC TGTGTGCCGG AGACTGGAGC CTGCCTGTGC
    CGCCCCGGCT TCATGGGCAG CCGCTGCCAG GATGCCTGCC CTGCTGGCTG GTTTGGACCT
    GGCTGCCAGA CGCAGTGCTC CTGTGCCAAC GATGGGCTCT GCCACCCAGT GACCGGCCGG
    TGCAGCTGCG CCCCTGGGTG GACTGGCCTC AGCTGCCAGA GAGCCTGTGA CAGCGGCCAC
    TGGGGCCCCG ACTGCAGCTT CACCTGCAAC TGCAGTGCAG GCCACGGGGG CTGTGACGCC
    GTCAGCGGCC TGTGTGTGTG TGAAGCCGGC TACGTGGGCC CGCGGTGTGA GCAGCGGTGT
    CCCCAGGGCT ACTTCGGGCC GGGCTGTGGG CGGCAGTGCC AGTGCGAGCA CGGGGCGGCC
    TGTGACCATG TCAGCGGGGC CTGCACCTGC CCTGCCGGCT GGAGGGGCAC CTTCTGTGAG
    CGAGCCTGCC CAGCCGGCTT CTTTGGAGTG GACTGTGGCC ATGCCTGTGA CTGCAGTGCC
    GGAGTCCCCT GTGATCCTGT GAGCGGCTCC TGCCTCTGTC CTTCCGGCCG CCAGGGCCCC
    CGCTGTGCCC AGACCTGCCC AGCCCACATG TTCGGCCACA ACTGCAGCCA GGCCTGCTCC
    TGCTTTAATG GGGCCTCCTG TGACCCTGTC CACGGGCAGT GCCGCTGTGG CCCTGGCTGG
    ACGGGGCCCA CCTGCCTGCA GGCCTGCCCA GAGGGCCTCT ATGGTGAGGA CTGTCAGCAC
    TCCTGCCTGT GCCAGAATGG GGGCAGCTGT GACCCTGTCT CAGGACACTG CACCTGCCCA
    GAGGGCTGGG CCGGCCTGGC CTGTGAGAAG GAGTGCCTCC CGGGGGTCTT TGAAGTTGGC
    TGCCCGCACA GCTGCGGGTG CCTCAACGGT GGCCTCTGTG ATCCGCACAC CGTCCACTGC
    CTCTGCCCAG CCGGCTGGAC CGGGGACAAG TGCCAGAGCT CCTGTCCCGA GGGCACCTTT
    GGGGTCCTCT GTGAGGAGCG CTGCGCCTGC CGGTGGGGGG CCGCCTGCCA CGCCGTCACT
    GGGGCCTGCC TCTGTCCCCC GGGGTGGAGG GGCTCGCGGT GTGAGAGCGC CTGCCCACCC
    AGCTGGTTTG GAGAGGCCTG TGCCCAGCGC TGCCAGTGCC CCCCGGGCGC CGCCTGCCAC
    CACGTCACCG GGGAGTGTCA CTGTCCCCCG GGCTTCACGG GGCCCAGCTG TGAGCAAGCC
    TGCCCACCTG GCACCTTTGG GGAGCGCTGT GGGCAGCAGT GCCACTGCCC CGGCGAGAAC
    CAGGCCTGCC ACCCCGCCTC AGGGACCTGC GCGTGTGCCC CCGGCTACCA CGGCGCCGGC
    TGCCAGCAAC GGTGCCCACC AGGGCGGTTT GGGCCTGGCT GTGAGCTGCT GTGTGGGTGT
    CTCAACGGGG GCTCTTGTGA CGCGGTCACT GGGGCCTGCC GCTGCCCCGC CGGATTCCTT
    GGGACTGACT GCAGCCTCGC CTGTCCACAC GGCCGCTTTG GCCCTGGCTG TGCCTACGTG
    TGCGGGTGTG GGCAGGGGGC GGCCTGTGAC CCCGTGACCG GCAGCTGCTC CTGTCCGCCT
    GGGAGGACCG GCGTCCACTG CGAGCACGGC TGCCCCCAGA ATCGGTTTGG TGTGAATTGT
    GAGCATGCGT GCTCCTGCAG GAATGGGGGC CTGTGTCACG CCACCAACGG CAGCTGTTCC
    TGTGGCCTGG GCTGGACTGG GCCACACTGC GAGCTGGCCT GCCCCGCCGG GCGCTACGGT
    GCCGCCTGCA GCCTCGAGTG CTCCTGCCAC AACCACGGGG CCTGTGAGCC CACGTCGGGG
    GCCTGCCGCT GCGGCCCCGG CTTCTACGGC CAGGCCTGCG AGCACTCCTG TCCCCCCGGC
    TTCCATGGGG CTGGCTGCCA GCAGACGTGC CAGTGTCAGC ATGGCGCCCC CTGCGACCCT
    GTCAGCGGCC AGTGCCTCTG CCCTGCTGGC CTCCATGGCC AGTTCTGCGA GCGGGGGTGT
    GAGCCGGGCT CGTTCGGAGA GGGCTGCGGC CAGCAGTGTG ACTGCGAGCT TGGGGTGCCC
    TGTGACCCCG TCACCGGCCA CTGCCTGTGC CCCCCTGGGC GCACAGGGAC CACCTGTGAC
    CTGGGCTGCA GGAGGGGCTT CTTCGGGCCG GGCTGTGCCC TGCACTGCTC CTGTGGGGGT
    GGAGCCGACT GCGACCCTGT CAGCGGGCAG TGCCTCTGCG TGGACGGCTA CACAGGGCCC
    ACGTGCCGGC AGGGTGGACC CTCCCAGCTC CCGGAGAGCC CGTCTCCAGC CCTGGGCCCA
    GCGGCCCTAC AACCAGGTTC CCACCAGACC CGGGCTCAGC AGCAGCCTAG AGAAGCACTA
    GCTCAGGGGC AGCCCCCACT GAGGCTTGGC TCCCAGGGGC CACTGAGGGG ACCTGGGGGC
    TTTGGTGTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92995
    Clone ID Related Accession (Same CDS sequence) XM_015475184.1 , XM_015466861.1
    Accession Version XM_015475184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGGTT TCTCTGGCAA ACCCACAGCT GGACAGAACA CAGGGGCCAT TCTCGGGGGG 
    ACGCAGGCAA GCGAGGATGG CTCCTTAGGG AGCAGGGCCC ATCCTGTGCC GAGCTGTCCC
    CAAGGGACAC TCCTGCCAGG TCACACTGGG ACACCACAGG ACCACAGGTG CTGGGCACGG
    GGAGGGACCT GGTTGGCGGC GCGGCCCCCC AGCTGCTGGA TTGACTCGGC GGGTCCCTCT
    CTCCTGAGGC CTGTCTGGAG GGGTGAGGTT TGCCCATCTC TGCCGGGCCT CGTCCTCCAG
    TGTTGTGGGG TCCTGAGCTT CCTCCTGCCC CTGTGCTGGG CTCACCCGCA TCTCCCCCTC
    GCAGATGTGA ATGAGTGTGC CACTGGCAAC GCAGGCTGTG AGGGTGAGTG TCACAACACC
    GTGGGGGGCT TCTACTGCCG CTGCCCGCCT GGCCGCCAGC TGCAGGGAGA CGGCAAGACC
    TGCCGAGACG TGGACGAGTG CCAGGTGCAC AATGGCGGCT GCCAGCACCG GTGTGTGAAC
    ACCCCAGGCT CCTACTTCTG CGAGTGCAAG CCCGGCTTCC GTCTCCACGC GGACGGCAGG
    ACCTGCCTGG CCATCAACTC CTGCGCTGTG GGCAATGGTG GCTGCCAGCA CAACTGCGTC
    CAGCTCACAG TGACCCAGCA CCGCTGCCAA TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT
    GGCAGGCGCT GCGTCCGGAG AAACCCATGC GCAGACAGGA ATGGCGGCTG CATGCACAGG
    TGCCGGGCAC TCCGGGGCCT TGCCCACTGC GAGTGCCGCG CGGGCTACCA GCTGGCAGTA
    GACCGCAAGG CCTGCGAAGA TGTGGATGAA TGTGCCACGG GCCTGGCCCA GTGTGCACAC
    GGCTGCCTCA ACACGCACGG CTCCTTCAAG TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT
    GCTGATGGCC GGCAGTGCTA CCGGATCGAG ATGGAGATTG TGAACAGCTG TGAGGCAGAC
    AACGGCGGCT GCTCCCACGG CTGCAGCCAC AGCAGCGCGG GCCCCGTCTG CACCTGCCCC
    CATGGCTACG AGCTGGATGA GGACCAGAGG ACGTGTATCG ACGTCGACGA CTGTGCCGCC
    TCCCCCTGCT GCCAGCAGGT TTGCACCAAC AGCCCCGGTG GCTACGAGTG CAGCTGCTAT
    GCTGGCTACC GGCTCAGTGC GGATGGCTGC GGGTGTGAGG ACGTGGACGA GTGCGCCTCT
    GGCCGCGGTG GCTGCGAGCA CCACTGCGCC AACCTGGCCG GCTCCTTCCA GTGCTCCTGT
    GAGGCCGGCT ACCAGCTGGA CGAGGACCGC AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG
    CTGGTCCTGG ACGGCCACCT CCCCCTCGGG CGCCCCCCTC TGCACGTCGC GGTGCTCCAG
    GACGAGCTGC CCCACCTCTT CCAAGACGAC TACGTCGGGG CTGAGGAGGA AGAGGCCGAG
    GCACGGGGCG AACACACACT GCAGGAAAAG TATGTCTGCC TGGACGACAC CTTTGGCCCA
    GACTGCAGCT TGACCTGTGA TGACTGCAGG AATGGGGGAA CCTGCCTCCC CACGTTGGAC
    AGCTGTGACT GCCCCGACGG CTGGACCGGG CTCGTCTGCA ACGAGACTTG TCCTCCGGAC
    ACCTTCGGGA AGAACTGCAG CTTCTCCTGC AGCTGTCAGA ACGGCGGGAC CTGTGACCCT
    GTGACGGGGG CCTGCCGCTG CCCCCCAGGG GTCGGCGGAG CCCACTGTGA GGACGGCTGC
    CCCAAAGGCT TCTATGGCAA GCACTGCCGT AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC
    TGCCACCGCC TCTACGGGGC CTGCCTCTGT GACCCGGGGC TCTACGGCCG CTTCTGCCAC
    CTGGCCTGCC CGCCATGGGC CTTTGGGCCA GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG
    CAGAATACGC GTGCGTGCGA TAAGAGGGAT GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG
    GGCGAGTGGT GCCAGGATGA GTGCCAGCCG GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG
    TGCACCTGCC CCCCGGGCGT GGCCTGCGAC CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT
    CCCGCTGGCT ACCACGGGGA GGACTGCGGC CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG
    AACTGCTCAG GCTCCTGCTC CTGTGGGGAC GCCCCCTGCG ACCGGGTCAC AGGGCAATGT
    CTGTGCCCCC CGGGGAGGAC CGGGGACGAC TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG
    GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG TGTGAGCACG GTGCCGCCTG TGTGCCGGAG
    ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT
    GCTGGCTGGT TTGGACCTGG CTGCCAGACG CAGTGCTCCT GTGCCAACGA TGGGCTCTGC
    CACCCAGTGA CCGGCCGGTG CAGCTGCGCC CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA
    GCCTGTGACA GCGGCCACTG GGGCCCCGAC TGCAGCTTCA CCTGCAACTG CAGTGCAGGC
    CACGGGGGCT GTGACGCCGT CAGCGGCCTG TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG
    CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG
    TGCGAGCACG GGGCGGCCTG TGACCATGTC AGCGGGGCCT GCACCTGCCC TGCCGGCTGG
    AGGGGCACCT TCTGTGAGCG AGCCTGCCCA GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT
    GCCTGTGACT GCAGTGCCGG AGTCCCCTGT GATCCTGTGA GCGGCTCCTG CCTCTGTCCT
    TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG ACCTGCCCAG CCCACATGTT CGGCCACAAC
    TGCAGCCAGG CCTGCTCCTG CTTTAATGGG GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC
    CGCTGTGGCC CTGGCTGGAC GGGGCCCACC TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT
    GGTGAGGACT GTCAGCACTC CTGCCTGTGC CAGAATGGGG GCAGCTGTGA CCCTGTCTCA
    GGACACTGCA CCTGCCCAGA GGGCTGGGCC GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG
    GGGGTCTTTG AAGTTGGCTG CCCGCACAGC TGCGGGTGCC TCAACGGTGG CCTCTGTGAT
    CCGCACACCG TCCACTGCCT CTGCCCAGCC GGCTGGACCG GGGACAAGTG CCAGAGCTCC
    TGTCCCGAGG GCACCTTTGG GGTCCTCTGT GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC
    GCCTGCCACG CCGTCACTGG GGCCTGCCTC TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT
    GAGAGCGCCT GCCCACCCAG CTGGTTTGGA GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC
    CCGGGCGCCG CCTGCCACCA CGTCACCGGG GAGTGTCACT GTCCCCCGGG CTTCACGGGG
    CCCAGCTGTG AGCAAGCCTG CCCACCTGGC ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC
    CACTGCCCCG GCGAGAACCA GGCCTGCCAC CCCGCCTCAG GGACCTGCGC GTGTGCCCCC
    GGCTACCACG GCGCCGGCTG CCAGCAACGG TGCCCACCAG GGCGGTTTGG GCCTGGCTGT
    GAGCTGCTGT GTGGGTGTCT CAACGGGGGC TCTTGTGACG CGGTCACTGG GGCCTGCCGC
    TGCCCCGCCG GATTCCTTGG GACTGACTGC AGCCTCGCCT GTCCACACGG CCGCTTTGGC
    CCTGGCTGTG CCTACGTGTG CGGGTGTGGG CAGGGGGCGG CCTGTGACCC CGTGACCGGC
    AGCTGCTCCT GTCCGCCTGG GAGGACCGGC GTCCACTGCG AGCACGGCTG CCCCCAGAAT
    CGGTTTGGTG TGAATTGTGA GCATGCGTGC TCCTGCAGGA ATGGGGGCCT GTGTCACGCC
    ACCAACGGCA GCTGTTCCTG TGGCCTGGGC TGGACTGGGC CACACTGCGA GCTGGCCTGC
    CCCGCCGGGC GCTACGGTGC CGCCTGCAGC CTCGAGTGCT CCTGCCACAA CCACGGGGCC
    TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC GGCCCCGGCT TCTACGGCCA GGCCTGCGAG
    CACTCCTGTC CCCCCGGCTT CCATGGGGCT GGCTGCCAGC AGACGTGCCA GTGTCAGCAT
    GGCGCCCCCT GCGACCCTGT CAGCGGCCAG TGCCTCTGCC CTGCTGGCCT CCATGGCCAG
    TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC
    TGCGAGCTTG GGGTGCCCTG TGACCCCGTC ACCGGCCACT GCCTGTGCCC CCCTGGGCGC
    ACAGGGACCA CCTGTGACCT GGGCTGCAGG AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG
    CACTGCTCCT GTGGGGGTGG AGCCGACTGC GACCCTGTCA GCGGGCAGTG CCTCTGCGTG
    GACGGCTACA CAGGGCCCAC GTGCCGGCAG GGTGGACCCT CCCAGCTCCC GGAGAGCCCG
    TCTCCAGCCC TGGGCCCAGC GGCCCTACAA CCAGGTTCCC ACCAGACCCG GGCTCAGCAG
    CAGCCTAGAG AAGCACTAGC TCAGGGGCAG CCCCCACTGA GGCTTGGCTC CCAGGGGCCA
    CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330670.1
    CDS25..4857
    Translation

    Target ORF information:

    RefSeq Version XM_015475184.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGGTT TCTCTGGCAA ACCCACAGCT GGACAGAACA CAGGGGCCAT TCTCGGGGGG 
    ACGCAGGCAA GCGAGGATGG CTCCTTAGGG AGCAGGGCCC ATCCTGTGCC GAGCTGTCCC
    CAAGGGACAC TCCTGCCAGG TCACACTGGG ACACCACAGG ACCACAGGTG CTGGGCACGG
    GGAGGGACCT GGTTGGCGGC GCGGCCCCCC AGCTGCTGGA TTGACTCGGC GGGTCCCTCT
    CTCCTGAGGC CTGTCTGGAG GGGTGAGGTT TGCCCATCTC TGCCGGGCCT CGTCCTCCAG
    TGTTGTGGGG TCCTGAGCTT CCTCCTGCCC CTGTGCTGGG CTCACCCGCA TCTCCCCCTC
    GCAGATGTGA ATGAGTGTGC CACTGGCAAC GCAGGCTGTG AGGGTGAGTG TCACAACACC
    GTGGGGGGCT TCTACTGCCG CTGCCCGCCT GGCCGCCAGC TGCAGGGAGA CGGCAAGACC
    TGCCGAGACG TGGACGAGTG CCAGGTGCAC AATGGCGGCT GCCAGCACCG GTGTGTGAAC
    ACCCCAGGCT CCTACTTCTG CGAGTGCAAG CCCGGCTTCC GTCTCCACGC GGACGGCAGG
    ACCTGCCTGG CCATCAACTC CTGCGCTGTG GGCAATGGTG GCTGCCAGCA CAACTGCGTC
    CAGCTCACAG TGACCCAGCA CCGCTGCCAA TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT
    GGCAGGCGCT GCGTCCGGAG AAACCCATGC GCAGACAGGA ATGGCGGCTG CATGCACAGG
    TGCCGGGCAC TCCGGGGCCT TGCCCACTGC GAGTGCCGCG CGGGCTACCA GCTGGCAGTA
    GACCGCAAGG CCTGCGAAGA TGTGGATGAA TGTGCCACGG GCCTGGCCCA GTGTGCACAC
    GGCTGCCTCA ACACGCACGG CTCCTTCAAG TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT
    GCTGATGGCC GGCAGTGCTA CCGGATCGAG ATGGAGATTG TGAACAGCTG TGAGGCAGAC
    AACGGCGGCT GCTCCCACGG CTGCAGCCAC AGCAGCGCGG GCCCCGTCTG CACCTGCCCC
    CATGGCTACG AGCTGGATGA GGACCAGAGG ACGTGTATCG ACGTCGACGA CTGTGCCGCC
    TCCCCCTGCT GCCAGCAGGT TTGCACCAAC AGCCCCGGTG GCTACGAGTG CAGCTGCTAT
    GCTGGCTACC GGCTCAGTGC GGATGGCTGC GGGTGTGAGG ACGTGGACGA GTGCGCCTCT
    GGCCGCGGTG GCTGCGAGCA CCACTGCGCC AACCTGGCCG GCTCCTTCCA GTGCTCCTGT
    GAGGCCGGCT ACCAGCTGGA CGAGGACCGC AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG
    CTGGTCCTGG ACGGCCACCT CCCCCTCGGG CGCCCCCCTC TGCACGTCGC GGTGCTCCAG
    GACGAGCTGC CCCACCTCTT CCAAGACGAC TACGTCGGGG CTGAGGAGGA AGAGGCCGAG
    GCACGGGGCG AACACACACT GCAGGAAAAG TATGTCTGCC TGGACGACAC CTTTGGCCCA
    GACTGCAGCT TGACCTGTGA TGACTGCAGG AATGGGGGAA CCTGCCTCCC CACGTTGGAC
    AGCTGTGACT GCCCCGACGG CTGGACCGGG CTCGTCTGCA ACGAGACTTG TCCTCCGGAC
    ACCTTCGGGA AGAACTGCAG CTTCTCCTGC AGCTGTCAGA ACGGCGGGAC CTGTGACCCT
    GTGACGGGGG CCTGCCGCTG CCCCCCAGGG GTCGGCGGAG CCCACTGTGA GGACGGCTGC
    CCCAAAGGCT TCTATGGCAA GCACTGCCGT AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC
    TGCCACCGCC TCTACGGGGC CTGCCTCTGT GACCCGGGGC TCTACGGCCG CTTCTGCCAC
    CTGGCCTGCC CGCCATGGGC CTTTGGGCCA GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG
    CAGAATACGC GTGCGTGCGA TAAGAGGGAT GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG
    GGCGAGTGGT GCCAGGATGA GTGCCAGCCG GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG
    TGCACCTGCC CCCCGGGCGT GGCCTGCGAC CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT
    CCCGCTGGCT ACCACGGGGA GGACTGCGGC CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG
    AACTGCTCAG GCTCCTGCTC CTGTGGGGAC GCCCCCTGCG ACCGGGTCAC AGGGCAATGT
    CTGTGCCCCC CGGGGAGGAC CGGGGACGAC TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG
    GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG TGTGAGCACG GTGCCGCCTG TGTGCCGGAG
    ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT
    GCTGGCTGGT TTGGACCTGG CTGCCAGACG CAGTGCTCCT GTGCCAACGA TGGGCTCTGC
    CACCCAGTGA CCGGCCGGTG CAGCTGCGCC CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA
    GCCTGTGACA GCGGCCACTG GGGCCCCGAC TGCAGCTTCA CCTGCAACTG CAGTGCAGGC
    CACGGGGGCT GTGACGCCGT CAGCGGCCTG TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG
    CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG
    TGCGAGCACG GGGCGGCCTG TGACCATGTC AGCGGGGCCT GCACCTGCCC TGCCGGCTGG
    AGGGGCACCT TCTGTGAGCG AGCCTGCCCA GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT
    GCCTGTGACT GCAGTGCCGG AGTCCCCTGT GATCCTGTGA GCGGCTCCTG CCTCTGTCCT
    TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG ACCTGCCCAG CCCACATGTT CGGCCACAAC
    TGCAGCCAGG CCTGCTCCTG CTTTAATGGG GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC
    CGCTGTGGCC CTGGCTGGAC GGGGCCCACC TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT
    GGTGAGGACT GTCAGCACTC CTGCCTGTGC CAGAATGGGG GCAGCTGTGA CCCTGTCTCA
    GGACACTGCA CCTGCCCAGA GGGCTGGGCC GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG
    GGGGTCTTTG AAGTTGGCTG CCCGCACAGC TGCGGGTGCC TCAACGGTGG CCTCTGTGAT
    CCGCACACCG TCCACTGCCT CTGCCCAGCC GGCTGGACCG GGGACAAGTG CCAGAGCTCC
    TGTCCCGAGG GCACCTTTGG GGTCCTCTGT GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC
    GCCTGCCACG CCGTCACTGG GGCCTGCCTC TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT
    GAGAGCGCCT GCCCACCCAG CTGGTTTGGA GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC
    CCGGGCGCCG CCTGCCACCA CGTCACCGGG GAGTGTCACT GTCCCCCGGG CTTCACGGGG
    CCCAGCTGTG AGCAAGCCTG CCCACCTGGC ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC
    CACTGCCCCG GCGAGAACCA GGCCTGCCAC CCCGCCTCAG GGACCTGCGC GTGTGCCCCC
    GGCTACCACG GCGCCGGCTG CCAGCAACGG TGCCCACCAG GGCGGTTTGG GCCTGGCTGT
    GAGCTGCTGT GTGGGTGTCT CAACGGGGGC TCTTGTGACG CGGTCACTGG GGCCTGCCGC
    TGCCCCGCCG GATTCCTTGG GACTGACTGC AGCCTCGCCT GTCCACACGG CCGCTTTGGC
    CCTGGCTGTG CCTACGTGTG CGGGTGTGGG CAGGGGGCGG CCTGTGACCC CGTGACCGGC
    AGCTGCTCCT GTCCGCCTGG GAGGACCGGC GTCCACTGCG AGCACGGCTG CCCCCAGAAT
    CGGTTTGGTG TGAATTGTGA GCATGCGTGC TCCTGCAGGA ATGGGGGCCT GTGTCACGCC
    ACCAACGGCA GCTGTTCCTG TGGCCTGGGC TGGACTGGGC CACACTGCGA GCTGGCCTGC
    CCCGCCGGGC GCTACGGTGC CGCCTGCAGC CTCGAGTGCT CCTGCCACAA CCACGGGGCC
    TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC GGCCCCGGCT TCTACGGCCA GGCCTGCGAG
    CACTCCTGTC CCCCCGGCTT CCATGGGGCT GGCTGCCAGC AGACGTGCCA GTGTCAGCAT
    GGCGCCCCCT GCGACCCTGT CAGCGGCCAG TGCCTCTGCC CTGCTGGCCT CCATGGCCAG
    TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC
    TGCGAGCTTG GGGTGCCCTG TGACCCCGTC ACCGGCCACT GCCTGTGCCC CCCTGGGCGC
    ACAGGGACCA CCTGTGACCT GGGCTGCAGG AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG
    CACTGCTCCT GTGGGGGTGG AGCCGACTGC GACCCTGTCA GCGGGCAGTG CCTCTGCGTG
    GACGGCTACA CAGGGCCCAC GTGCCGGCAG GGTGGACCCT CCCAGCTCCC GGAGAGCCCG
    TCTCCAGCCC TGGGCCCAGC GGCCCTACAA CCAGGTTCCC ACCAGACCCG GGCTCAGCAG
    CAGCCTAGAG AAGCACTAGC TCAGGGGCAG CCCCCACTGA GGCTTGGCTC CCAGGGGCCA
    CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92995
    Clone ID Related Accession (Same CDS sequence) XM_015475184.1 , XM_015466861.1
    Accession Version XM_015466861.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGGTT TCTCTGGCAA ACCCACAGCT GGACAGAACA CAGGGGCCAT TCTCGGGGGG 
    ACGCAGGCAA GCGAGGATGG CTCCTTAGGG AGCAGGGCCC ATCCTGTGCC GAGCTGTCCC
    CAAGGGACAC TCCTGCCAGG TCACACTGGG ACACCACAGG ACCACAGGTG CTGGGCACGG
    GGAGGGACCT GGTTGGCGGC GCGGCCCCCC AGCTGCTGGA TTGACTCGGC GGGTCCCTCT
    CTCCTGAGGC CTGTCTGGAG GGGTGAGGTT TGCCCATCTC TGCCGGGCCT CGTCCTCCAG
    TGTTGTGGGG TCCTGAGCTT CCTCCTGCCC CTGTGCTGGG CTCACCCGCA TCTCCCCCTC
    GCAGATGTGA ATGAGTGTGC CACTGGCAAC GCAGGCTGTG AGGGTGAGTG TCACAACACC
    GTGGGGGGCT TCTACTGCCG CTGCCCGCCT GGCCGCCAGC TGCAGGGAGA CGGCAAGACC
    TGCCGAGACG TGGACGAGTG CCAGGTGCAC AATGGCGGCT GCCAGCACCG GTGTGTGAAC
    ACCCCAGGCT CCTACTTCTG CGAGTGCAAG CCCGGCTTCC GTCTCCACGC GGACGGCAGG
    ACCTGCCTGG CCATCAACTC CTGCGCTGTG GGCAATGGTG GCTGCCAGCA CAACTGCGTC
    CAGCTCACAG TGACCCAGCA CCGCTGCCAA TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT
    GGCAGGCGCT GCGTCCGGAG AAACCCATGC GCAGACAGGA ATGGCGGCTG CATGCACAGG
    TGCCGGGCAC TCCGGGGCCT TGCCCACTGC GAGTGCCGCG CGGGCTACCA GCTGGCAGTA
    GACCGCAAGG CCTGCGAAGA TGTGGATGAA TGTGCCACGG GCCTGGCCCA GTGTGCACAC
    GGCTGCCTCA ACACGCACGG CTCCTTCAAG TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT
    GCTGATGGCC GGCAGTGCTA CCGGATCGAG ATGGAGATTG TGAACAGCTG TGAGGCAGAC
    AACGGCGGCT GCTCCCACGG CTGCAGCCAC AGCAGCGCGG GCCCCGTCTG CACCTGCCCC
    CATGGCTACG AGCTGGATGA GGACCAGAGG ACGTGTATCG ACGTCGACGA CTGTGCCGCC
    TCCCCCTGCT GCCAGCAGGT TTGCACCAAC AGCCCCGGTG GCTACGAGTG CAGCTGCTAT
    GCTGGCTACC GGCTCAGTGC GGATGGCTGC GGGTGTGAGG ACGTGGACGA GTGCGCCTCT
    GGCCGCGGTG GCTGCGAGCA CCACTGCGCC AACCTGGCCG GCTCCTTCCA GTGCTCCTGT
    GAGGCCGGCT ACCAGCTGGA CGAGGACCGC AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG
    CTGGTCCTGG ACGGCCACCT CCCCCTCGGG CGCCCCCCTC TGCACGTCGC GGTGCTCCAG
    GACGAGCTGC CCCACCTCTT CCAAGACGAC TACGTCGGGG CTGAGGAGGA AGAGGCCGAG
    GCACGGGGCG AACACACACT GCAGGAAAAG TATGTCTGCC TGGACGACAC CTTTGGCCCA
    GACTGCAGCT TGACCTGTGA TGACTGCAGG AATGGGGGAA CCTGCCTCCC CACGTTGGAC
    AGCTGTGACT GCCCCGACGG CTGGACCGGG CTCGTCTGCA ACGAGACTTG TCCTCCGGAC
    ACCTTCGGGA AGAACTGCAG CTTCTCCTGC AGCTGTCAGA ACGGCGGGAC CTGTGACCCT
    GTGACGGGGG CCTGCCGCTG CCCCCCAGGG GTCGGCGGAG CCCACTGTGA GGACGGCTGC
    CCCAAAGGCT TCTATGGCAA GCACTGCCGT AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC
    TGCCACCGCC TCTACGGGGC CTGCCTCTGT GACCCGGGGC TCTACGGCCG CTTCTGCCAC
    CTGGCCTGCC CGCCATGGGC CTTTGGGCCA GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG
    CAGAATACGC GTGCGTGCGA TAAGAGGGAT GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG
    GGCGAGTGGT GCCAGGATGA GTGCCAGCCG GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG
    TGCACCTGCC CCCCGGGCGT GGCCTGCGAC CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT
    CCCGCTGGCT ACCACGGGGA GGACTGCGGC CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG
    AACTGCTCAG GCTCCTGCTC CTGTGGGGAC GCCCCCTGCG ACCGGGTCAC AGGGCAATGT
    CTGTGCCCCC CGGGGAGGAC CGGGGACGAC TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG
    GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG TGTGAGCACG GTGCCGCCTG TGTGCCGGAG
    ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT
    GCTGGCTGGT TTGGACCTGG CTGCCAGACG CAGTGCTCCT GTGCCAACGA TGGGCTCTGC
    CACCCAGTGA CCGGCCGGTG CAGCTGCGCC CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA
    GCCTGTGACA GCGGCCACTG GGGCCCCGAC TGCAGCTTCA CCTGCAACTG CAGTGCAGGC
    CACGGGGGCT GTGACGCCGT CAGCGGCCTG TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG
    CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG
    TGCGAGCACG GGGCGGCCTG TGACCATGTC AGCGGGGCCT GCACCTGCCC TGCCGGCTGG
    AGGGGCACCT TCTGTGAGCG AGCCTGCCCA GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT
    GCCTGTGACT GCAGTGCCGG AGTCCCCTGT GATCCTGTGA GCGGCTCCTG CCTCTGTCCT
    TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG ACCTGCCCAG CCCACATGTT CGGCCACAAC
    TGCAGCCAGG CCTGCTCCTG CTTTAATGGG GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC
    CGCTGTGGCC CTGGCTGGAC GGGGCCCACC TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT
    GGTGAGGACT GTCAGCACTC CTGCCTGTGC CAGAATGGGG GCAGCTGTGA CCCTGTCTCA
    GGACACTGCA CCTGCCCAGA GGGCTGGGCC GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG
    GGGGTCTTTG AAGTTGGCTG CCCGCACAGC TGCGGGTGCC TCAACGGTGG CCTCTGTGAT
    CCGCACACCG TCCACTGCCT CTGCCCAGCC GGCTGGACCG GGGACAAGTG CCAGAGCTCC
    TGTCCCGAGG GCACCTTTGG GGTCCTCTGT GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC
    GCCTGCCACG CCGTCACTGG GGCCTGCCTC TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT
    GAGAGCGCCT GCCCACCCAG CTGGTTTGGA GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC
    CCGGGCGCCG CCTGCCACCA CGTCACCGGG GAGTGTCACT GTCCCCCGGG CTTCACGGGG
    CCCAGCTGTG AGCAAGCCTG CCCACCTGGC ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC
    CACTGCCCCG GCGAGAACCA GGCCTGCCAC CCCGCCTCAG GGACCTGCGC GTGTGCCCCC
    GGCTACCACG GCGCCGGCTG CCAGCAACGG TGCCCACCAG GGCGGTTTGG GCCTGGCTGT
    GAGCTGCTGT GTGGGTGTCT CAACGGGGGC TCTTGTGACG CGGTCACTGG GGCCTGCCGC
    TGCCCCGCCG GATTCCTTGG GACTGACTGC AGCCTCGCCT GTCCACACGG CCGCTTTGGC
    CCTGGCTGTG CCTACGTGTG CGGGTGTGGG CAGGGGGCGG CCTGTGACCC CGTGACCGGC
    AGCTGCTCCT GTCCGCCTGG GAGGACCGGC GTCCACTGCG AGCACGGCTG CCCCCAGAAT
    CGGTTTGGTG TGAATTGTGA GCATGCGTGC TCCTGCAGGA ATGGGGGCCT GTGTCACGCC
    ACCAACGGCA GCTGTTCCTG TGGCCTGGGC TGGACTGGGC CACACTGCGA GCTGGCCTGC
    CCCGCCGGGC GCTACGGTGC CGCCTGCAGC CTCGAGTGCT CCTGCCACAA CCACGGGGCC
    TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC GGCCCCGGCT TCTACGGCCA GGCCTGCGAG
    CACTCCTGTC CCCCCGGCTT CCATGGGGCT GGCTGCCAGC AGACGTGCCA GTGTCAGCAT
    GGCGCCCCCT GCGACCCTGT CAGCGGCCAG TGCCTCTGCC CTGCTGGCCT CCATGGCCAG
    TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC
    TGCGAGCTTG GGGTGCCCTG TGACCCCGTC ACCGGCCACT GCCTGTGCCC CCCTGGGCGC
    ACAGGGACCA CCTGTGACCT GGGCTGCAGG AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG
    CACTGCTCCT GTGGGGGTGG AGCCGACTGC GACCCTGTCA GCGGGCAGTG CCTCTGCGTG
    GACGGCTACA CAGGGCCCAC GTGCCGGCAG GGTGGACCCT CCCAGCTCCC GGAGAGCCCG
    TCTCCAGCCC TGGGCCCAGC GGCCCTACAA CCAGGTTCCC ACCAGACCCG GGCTCAGCAG
    CAGCCTAGAG AAGCACTAGC TCAGGGGCAG CCCCCACTGA GGCTTGGCTC CCAGGGGCCA
    CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322347.1
    CDS25..4857
    Translation

    Target ORF information:

    RefSeq Version XM_015466861.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 6 (MEGF6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTGGTT TCTCTGGCAA ACCCACAGCT GGACAGAACA CAGGGGCCAT TCTCGGGGGG 
    ACGCAGGCAA GCGAGGATGG CTCCTTAGGG AGCAGGGCCC ATCCTGTGCC GAGCTGTCCC
    CAAGGGACAC TCCTGCCAGG TCACACTGGG ACACCACAGG ACCACAGGTG CTGGGCACGG
    GGAGGGACCT GGTTGGCGGC GCGGCCCCCC AGCTGCTGGA TTGACTCGGC GGGTCCCTCT
    CTCCTGAGGC CTGTCTGGAG GGGTGAGGTT TGCCCATCTC TGCCGGGCCT CGTCCTCCAG
    TGTTGTGGGG TCCTGAGCTT CCTCCTGCCC CTGTGCTGGG CTCACCCGCA TCTCCCCCTC
    GCAGATGTGA ATGAGTGTGC CACTGGCAAC GCAGGCTGTG AGGGTGAGTG TCACAACACC
    GTGGGGGGCT TCTACTGCCG CTGCCCGCCT GGCCGCCAGC TGCAGGGAGA CGGCAAGACC
    TGCCGAGACG TGGACGAGTG CCAGGTGCAC AATGGCGGCT GCCAGCACCG GTGTGTGAAC
    ACCCCAGGCT CCTACTTCTG CGAGTGCAAG CCCGGCTTCC GTCTCCACGC GGACGGCAGG
    ACCTGCCTGG CCATCAACTC CTGCGCTGTG GGCAATGGTG GCTGCCAGCA CAACTGCGTC
    CAGCTCACAG TGACCCAGCA CCGCTGCCAA TGCCGGCCCG AGTTCCAGCT CCAGGAGGAT
    GGCAGGCGCT GCGTCCGGAG AAACCCATGC GCAGACAGGA ATGGCGGCTG CATGCACAGG
    TGCCGGGCAC TCCGGGGCCT TGCCCACTGC GAGTGCCGCG CGGGCTACCA GCTGGCAGTA
    GACCGCAAGG CCTGCGAAGA TGTGGATGAA TGTGCCACGG GCCTGGCCCA GTGTGCACAC
    GGCTGCCTCA ACACGCACGG CTCCTTCAAG TGTGTGTGCA ATGCGGGCTA CGAGCTGGGT
    GCTGATGGCC GGCAGTGCTA CCGGATCGAG ATGGAGATTG TGAACAGCTG TGAGGCAGAC
    AACGGCGGCT GCTCCCACGG CTGCAGCCAC AGCAGCGCGG GCCCCGTCTG CACCTGCCCC
    CATGGCTACG AGCTGGATGA GGACCAGAGG ACGTGTATCG ACGTCGACGA CTGTGCCGCC
    TCCCCCTGCT GCCAGCAGGT TTGCACCAAC AGCCCCGGTG GCTACGAGTG CAGCTGCTAT
    GCTGGCTACC GGCTCAGTGC GGATGGCTGC GGGTGTGAGG ACGTGGACGA GTGCGCCTCT
    GGCCGCGGTG GCTGCGAGCA CCACTGCGCC AACCTGGCCG GCTCCTTCCA GTGCTCCTGT
    GAGGCCGGCT ACCAGCTGGA CGAGGACCGC AGGGGTTGCA CCTCCCTGGA GCTGCCCGAG
    CTGGTCCTGG ACGGCCACCT CCCCCTCGGG CGCCCCCCTC TGCACGTCGC GGTGCTCCAG
    GACGAGCTGC CCCACCTCTT CCAAGACGAC TACGTCGGGG CTGAGGAGGA AGAGGCCGAG
    GCACGGGGCG AACACACACT GCAGGAAAAG TATGTCTGCC TGGACGACAC CTTTGGCCCA
    GACTGCAGCT TGACCTGTGA TGACTGCAGG AATGGGGGAA CCTGCCTCCC CACGTTGGAC
    AGCTGTGACT GCCCCGACGG CTGGACCGGG CTCGTCTGCA ACGAGACTTG TCCTCCGGAC
    ACCTTCGGGA AGAACTGCAG CTTCTCCTGC AGCTGTCAGA ACGGCGGGAC CTGTGACCCT
    GTGACGGGGG CCTGCCGCTG CCCCCCAGGG GTCGGCGGAG CCCACTGTGA GGACGGCTGC
    CCCAAAGGCT TCTATGGCAA GCACTGCCGT AAGAAGTGCC ACTGTGCCAA CCGGGGCCGC
    TGCCACCGCC TCTACGGGGC CTGCCTCTGT GACCCGGGGC TCTACGGCCG CTTCTGCCAC
    CTGGCCTGCC CGCCATGGGC CTTTGGGCCA GGCTGCTCAG AGGAGTGCAA GTGTGAGCAG
    CAGAATACGC GTGCGTGCGA TAAGAGGGAT GGCAGCTGTG CCTGCAAGGC GGGCTTCAGG
    GGCGAGTGGT GCCAGGATGA GTGCCAGCCG GGCTTTTTTG GGCCAGGGTG CCGGCAGGCG
    TGCACCTGCC CCCCGGGCGT GGCCTGCGAC CCTGTCAGTG GCGAGTGTGG GAAGCAGTGT
    CCCGCTGGCT ACCACGGGGA GGACTGCGGC CAAGAGTGCC CAGCAGGGAC GTTTGGCCAG
    AACTGCTCAG GCTCCTGCTC CTGTGGGGAC GCCCCCTGCG ACCGGGTCAC AGGGCAATGT
    CTGTGCCCCC CGGGGAGGAC CGGGGACGAC TGTGGGGCAG ATTGTCCCGA GGGCCGCTGG
    GGGCTTGGCT GCCAGGAGAT CTGCCCGCCG TGTGAGCACG GTGCCGCCTG TGTGCCGGAG
    ACTGGAGCCT GCCTGTGCCG CCCCGGCTTC ATGGGCAGCC GCTGCCAGGA TGCCTGCCCT
    GCTGGCTGGT TTGGACCTGG CTGCCAGACG CAGTGCTCCT GTGCCAACGA TGGGCTCTGC
    CACCCAGTGA CCGGCCGGTG CAGCTGCGCC CCTGGGTGGA CTGGCCTCAG CTGCCAGAGA
    GCCTGTGACA GCGGCCACTG GGGCCCCGAC TGCAGCTTCA CCTGCAACTG CAGTGCAGGC
    CACGGGGGCT GTGACGCCGT CAGCGGCCTG TGTGTGTGTG AAGCCGGCTA CGTGGGCCCG
    CGGTGTGAGC AGCGGTGTCC CCAGGGCTAC TTCGGGCCGG GCTGTGGGCG GCAGTGCCAG
    TGCGAGCACG GGGCGGCCTG TGACCATGTC AGCGGGGCCT GCACCTGCCC TGCCGGCTGG
    AGGGGCACCT TCTGTGAGCG AGCCTGCCCA GCCGGCTTCT TTGGAGTGGA CTGTGGCCAT
    GCCTGTGACT GCAGTGCCGG AGTCCCCTGT GATCCTGTGA GCGGCTCCTG CCTCTGTCCT
    TCCGGCCGCC AGGGCCCCCG CTGTGCCCAG ACCTGCCCAG CCCACATGTT CGGCCACAAC
    TGCAGCCAGG CCTGCTCCTG CTTTAATGGG GCCTCCTGTG ACCCTGTCCA CGGGCAGTGC
    CGCTGTGGCC CTGGCTGGAC GGGGCCCACC TGCCTGCAGG CCTGCCCAGA GGGCCTCTAT
    GGTGAGGACT GTCAGCACTC CTGCCTGTGC CAGAATGGGG GCAGCTGTGA CCCTGTCTCA
    GGACACTGCA CCTGCCCAGA GGGCTGGGCC GGCCTGGCCT GTGAGAAGGA GTGCCTCCCG
    GGGGTCTTTG AAGTTGGCTG CCCGCACAGC TGCGGGTGCC TCAACGGTGG CCTCTGTGAT
    CCGCACACCG TCCACTGCCT CTGCCCAGCC GGCTGGACCG GGGACAAGTG CCAGAGCTCC
    TGTCCCGAGG GCACCTTTGG GGTCCTCTGT GAGGAGCGCT GCGCCTGCCG GTGGGGGGCC
    GCCTGCCACG CCGTCACTGG GGCCTGCCTC TGTCCCCCGG GGTGGAGGGG CTCGCGGTGT
    GAGAGCGCCT GCCCACCCAG CTGGTTTGGA GAGGCCTGTG CCCAGCGCTG CCAGTGCCCC
    CCGGGCGCCG CCTGCCACCA CGTCACCGGG GAGTGTCACT GTCCCCCGGG CTTCACGGGG
    CCCAGCTGTG AGCAAGCCTG CCCACCTGGC ACCTTTGGGG AGCGCTGTGG GCAGCAGTGC
    CACTGCCCCG GCGAGAACCA GGCCTGCCAC CCCGCCTCAG GGACCTGCGC GTGTGCCCCC
    GGCTACCACG GCGCCGGCTG CCAGCAACGG TGCCCACCAG GGCGGTTTGG GCCTGGCTGT
    GAGCTGCTGT GTGGGTGTCT CAACGGGGGC TCTTGTGACG CGGTCACTGG GGCCTGCCGC
    TGCCCCGCCG GATTCCTTGG GACTGACTGC AGCCTCGCCT GTCCACACGG CCGCTTTGGC
    CCTGGCTGTG CCTACGTGTG CGGGTGTGGG CAGGGGGCGG CCTGTGACCC CGTGACCGGC
    AGCTGCTCCT GTCCGCCTGG GAGGACCGGC GTCCACTGCG AGCACGGCTG CCCCCAGAAT
    CGGTTTGGTG TGAATTGTGA GCATGCGTGC TCCTGCAGGA ATGGGGGCCT GTGTCACGCC
    ACCAACGGCA GCTGTTCCTG TGGCCTGGGC TGGACTGGGC CACACTGCGA GCTGGCCTGC
    CCCGCCGGGC GCTACGGTGC CGCCTGCAGC CTCGAGTGCT CCTGCCACAA CCACGGGGCC
    TGTGAGCCCA CGTCGGGGGC CTGCCGCTGC GGCCCCGGCT TCTACGGCCA GGCCTGCGAG
    CACTCCTGTC CCCCCGGCTT CCATGGGGCT GGCTGCCAGC AGACGTGCCA GTGTCAGCAT
    GGCGCCCCCT GCGACCCTGT CAGCGGCCAG TGCCTCTGCC CTGCTGGCCT CCATGGCCAG
    TTCTGCGAGC GGGGGTGTGA GCCGGGCTCG TTCGGAGAGG GCTGCGGCCA GCAGTGTGAC
    TGCGAGCTTG GGGTGCCCTG TGACCCCGTC ACCGGCCACT GCCTGTGCCC CCCTGGGCGC
    ACAGGGACCA CCTGTGACCT GGGCTGCAGG AGGGGCTTCT TCGGGCCGGG CTGTGCCCTG
    CACTGCTCCT GTGGGGGTGG AGCCGACTGC GACCCTGTCA GCGGGCAGTG CCTCTGCGTG
    GACGGCTACA CAGGGCCCAC GTGCCGGCAG GGTGGACCCT CCCAGCTCCC GGAGAGCCCG
    TCTCCAGCCC TGGGCCCAGC GGCCCTACAA CCAGGTTCCC ACCAGACCCG GGCTCAGCAG
    CAGCCTAGAG AAGCACTAGC TCAGGGGCAG CCCCCACTGA GGCTTGGCTC CCAGGGGCCA
    CTGAGGGGAC CTGGGGGCTT TGGTGTCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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