MAGI1 cDNA ORF clone, Bos taurus(cattle)

The following MAGI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa11007 XM_024983156.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11008 XM_024983159.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11009 XM_024983160.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11010 XM_024983161.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11011 XM_024983162.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11012 XM_024983163.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11013 XM_024983164.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11014 XM_024983170.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11015 XM_024983171.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa11016 XM_024983173.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa11017 XM_024983172.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa11018 XM_002696963.6
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa11019 XM_024983178.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa229247 XM_024983165.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa229248 XM_024983167.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa229249 XM_024983168.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa229250 XM_024983175.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229251 XM_024983176.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa57400 XM_024983157.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa57401 XM_024983158.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa57402 XM_024983174.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa57403 XM_024983169.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa31469 XM_001789358.6
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa31469 XM_002696963.5
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa11007
    Clone ID Related Accession (Same CDS sequence) XM_024983156.1
    Accession Version XM_024983156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGCA
    GGCAATGCAT GTGTGGCCAT GCCTCACAAA ATAGGTCGGA TTATTGAGGG GAGCCCTGCT
    GACCGATGCG GCAAGCTGAA AGTGGGAGAC CGGATCTTGG CAGTAAATGG ATGTTCCATC
    ACCAACAAAT CCCACTCTGA CATTGTCAAC CTCATCAAGG AAGCGGGCAA CACAGTCACC
    CTCCGCATCA TCCCTGGGGA TGAGTCTTCA AATGCCACTC TGCTGACCAA TGCGGAGAAG
    ATAGCCACCA TCACCACCAC ACACACCCCT TCGCAGCAAG GAGCCCAGGA GACCAGGAAT
    ACCACCAAAC CAAAGCCAGA ATCTCAGTTT GAATTCAAAG CACCCCAGGC AACACAGGAG
    CAAGATTTTT ACACTGTGGA GCTGGAAAGA GGAGCCAAGG GATTTGGTTT CAGTCTTCGA
    GGAGGTCGAG AGTATAACAT GGACCTCTAC GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC
    GAAAGGTGTG GGAAGATGAA GGTTGGTGAT GAAATTTTAG AGATCAATGG TGAGACCACC
    AAAAACATGA AGCATTCTCG GGCTATAGAA CTGATTAAGA ATGGTGGCCG CAGAGTGCGT
    CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC CCAGAATATG ACCCCGGCAG CGACGGGAAC
    GGCCCCCCCA CCGGTCCACA AGGTGTTCCG GAAATGAGGG CTGGGCCCCC GGACCGCCGA
    CAGCATCCAT CATTGGAGTC CAGCTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
    GAAAAGCGGG CACACGTGAG AGATCCCAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
    GAACATCACA CGTGGAACGG AACTTCTAGA AAACCCGACA GTGGGGCTTG CCGACCCAAG
    GACCGGGCAC CCGAGGGACG AAGAGACGCC CAACCCGAGC GGATGGTGAC CAATGGCCCC
    AAAAGGAGGT CTCCGGAGAA GCGGAGGGAA GGCACGCGCA GTGCTGACAA CACTTTGGAG
    AGAAGGGAGA AGCAGGAGAA GAGGAAGGAG GTTTCCCCGG AGAGGAGGCG AGAGCGGTCC
    CCGACCCGCC GGAGGGATGG CTCTCCAGGC CGGCGGCGGC GGTCTCTGGA GAGACTCTTG
    GATCAGAGAA GATCGCCCGA GCGCAAGAGA GGAAGCTCGC CCGAAAGGAG AGCCAAATCC
    ACCGACCGGA GGCGGACCAA GTCCCCCGAG CGGAGGAGAG AGCGGTCCCT GGAGAAAAGG
    AACAAAGAGG ACAGAGGCAG CCACCGGGAG AGGGACGAGA CGAGTCTAAG ACAGGACACC
    GGCCGGAGTT CCAGACATCC CCCGGAGCAG CGGAGAAGAC CTTACAAAGA ATGCAGCACC
    GACCTCAGTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838924.1
    CDS618..5072
    Translation

    Target ORF information:

    RefSeq Version XM_024983156.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGCA
    GGCAATGCAT GTGTGGCCAT GCCTCACAAA ATAGGTCGGA TTATTGAGGG GAGCCCTGCT
    GACCGATGCG GCAAGCTGAA AGTGGGAGAC CGGATCTTGG CAGTAAATGG ATGTTCCATC
    ACCAACAAAT CCCACTCTGA CATTGTCAAC CTCATCAAGG AAGCGGGCAA CACAGTCACC
    CTCCGCATCA TCCCTGGGGA TGAGTCTTCA AATGCCACTC TGCTGACCAA TGCGGAGAAG
    ATAGCCACCA TCACCACCAC ACACACCCCT TCGCAGCAAG GAGCCCAGGA GACCAGGAAT
    ACCACCAAAC CAAAGCCAGA ATCTCAGTTT GAATTCAAAG CACCCCAGGC AACACAGGAG
    CAAGATTTTT ACACTGTGGA GCTGGAAAGA GGAGCCAAGG GATTTGGTTT CAGTCTTCGA
    GGAGGTCGAG AGTATAACAT GGACCTCTAC GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC
    GAAAGGTGTG GGAAGATGAA GGTTGGTGAT GAAATTTTAG AGATCAATGG TGAGACCACC
    AAAAACATGA AGCATTCTCG GGCTATAGAA CTGATTAAGA ATGGTGGCCG CAGAGTGCGT
    CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC CCAGAATATG ACCCCGGCAG CGACGGGAAC
    GGCCCCCCCA CCGGTCCACA AGGTGTTCCG GAAATGAGGG CTGGGCCCCC GGACCGCCGA
    CAGCATCCAT CATTGGAGTC CAGCTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
    GAAAAGCGGG CACACGTGAG AGATCCCAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
    GAACATCACA CGTGGAACGG AACTTCTAGA AAACCCGACA GTGGGGCTTG CCGACCCAAG
    GACCGGGCAC CCGAGGGACG AAGAGACGCC CAACCCGAGC GGATGGTGAC CAATGGCCCC
    AAAAGGAGGT CTCCGGAGAA GCGGAGGGAA GGCACGCGCA GTGCTGACAA CACTTTGGAG
    AGAAGGGAGA AGCAGGAGAA GAGGAAGGAG GTTTCCCCGG AGAGGAGGCG AGAGCGGTCC
    CCGACCCGCC GGAGGGATGG CTCTCCAGGC CGGCGGCGGC GGTCTCTGGA GAGACTCTTG
    GATCAGAGAA GATCGCCCGA GCGCAAGAGA GGAAGCTCGC CCGAAAGGAG AGCCAAATCC
    ACCGACCGGA GGCGGACCAA GTCCCCCGAG CGGAGGAGAG AGCGGTCCCT GGAGAAAAGG
    AACAAAGAGG ACAGAGGCAG CCACCGGGAG AGGGACGAGA CGAGTCTAAG ACAGGACACC
    GGCCGGAGTT CCAGACATCC CCCGGAGCAG CGGAGAAGAC CTTACAAAGA ATGCAGCACC
    GACCTCAGTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11008
    Clone ID Related Accession (Same CDS sequence) XM_024983159.1
    Accession Version XM_024983159.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGGT
    CGGATTATTG AGGGGAGCCC TGCTGACCGA TGCGGCAAGC TGAAAGTGGG AGACCGGATC
    TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCACT CTGACATTGT CAACCTCATC
    AAGGAAGCGG GCAACACAGT CACCCTCCGC ATCATCCCTG GGGATGAGTC TTCAAATGCC
    ACTCTGCTGA CCAATGCGGA GAAGATAGCC ACCATCACCA CCACACACAC CCCTTCGCAG
    CAAGGAGCCC AGGAGACCAG GAATACCACC AAACCAAAGC CAGAATCTCA GTTTGAATTC
    AAAGCACCCC AGGCAACACA GGAGCAAGAT TTTTACACTG TGGAGCTGGA AAGAGGAGCC
    AAGGGATTTG GTTTCAGTCT TCGAGGAGGT CGAGAGTATA ACATGGACCT CTACGTTCTG
    CGCTTAGCAG AGGATGGTCC TGCCGAAAGG TGTGGGAAGA TGAAGGTTGG TGATGAAATT
    TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGGGCTAT AGAACTGATT
    AAGAATGGTG GCCGCAGAGT GCGTCTGTTT CTGAAGCGGG GAGACGGCTC GGTCCCAGAA
    TATGACCCCG GCAGCGACGG GAACGGCCCC CCCACCGGTC CACAAGGTGT TCCGGAAATG
    AGGGCTGGGC CCCCGGACCG CCGACAGCAT CCATCATTGG AGTCCAGCTA CCCACCCGAT
    CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG TGAGAGATCC CAAAGGCAGC
    AGAGAGTATA GCAGACAACC CAATGAACAT CACACGTGGA ACGGAACTTC TAGAAAACCC
    GACAGTGGGG CTTGCCGACC CAAGGACCGG GCACCCGAGG GACGAAGAGA CGCCCAACCC
    GAGCGGATGG TGACCAATGG CCCCAAAAGG AGGTCTCCGG AGAAGCGGAG GGAAGGCACG
    CGCAGTGCTG ACAACACTTT GGAGAGAAGG GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC
    CCGGAGAGGA GGCGAGAGCG GTCCCCGACC CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG
    CGGCGGTCTC TGGAGAGACT CTTGGATCAG AGAAGATCGC CCGAGCGCAA GAGAGGAAGC
    TCGCCCGAAA GGAGAGCCAA ATCCACCGAC CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG
    AGAGAGCGGT CCCTGGAGAA AAGGAACAAA GAGGACAGAG GCAGCCACCG GGAGAGGGAC
    GAGACGAGTC TAAGACAGGA CACCGGCCGG AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA
    AGACCTTACA AAGAATGCAG CACCGACCTC AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838927.1
    CDS618..5036
    Translation

    Target ORF information:

    RefSeq Version XM_024983159.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983159.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGGT
    CGGATTATTG AGGGGAGCCC TGCTGACCGA TGCGGCAAGC TGAAAGTGGG AGACCGGATC
    TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCACT CTGACATTGT CAACCTCATC
    AAGGAAGCGG GCAACACAGT CACCCTCCGC ATCATCCCTG GGGATGAGTC TTCAAATGCC
    ACTCTGCTGA CCAATGCGGA GAAGATAGCC ACCATCACCA CCACACACAC CCCTTCGCAG
    CAAGGAGCCC AGGAGACCAG GAATACCACC AAACCAAAGC CAGAATCTCA GTTTGAATTC
    AAAGCACCCC AGGCAACACA GGAGCAAGAT TTTTACACTG TGGAGCTGGA AAGAGGAGCC
    AAGGGATTTG GTTTCAGTCT TCGAGGAGGT CGAGAGTATA ACATGGACCT CTACGTTCTG
    CGCTTAGCAG AGGATGGTCC TGCCGAAAGG TGTGGGAAGA TGAAGGTTGG TGATGAAATT
    TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGGGCTAT AGAACTGATT
    AAGAATGGTG GCCGCAGAGT GCGTCTGTTT CTGAAGCGGG GAGACGGCTC GGTCCCAGAA
    TATGACCCCG GCAGCGACGG GAACGGCCCC CCCACCGGTC CACAAGGTGT TCCGGAAATG
    AGGGCTGGGC CCCCGGACCG CCGACAGCAT CCATCATTGG AGTCCAGCTA CCCACCCGAT
    CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG TGAGAGATCC CAAAGGCAGC
    AGAGAGTATA GCAGACAACC CAATGAACAT CACACGTGGA ACGGAACTTC TAGAAAACCC
    GACAGTGGGG CTTGCCGACC CAAGGACCGG GCACCCGAGG GACGAAGAGA CGCCCAACCC
    GAGCGGATGG TGACCAATGG CCCCAAAAGG AGGTCTCCGG AGAAGCGGAG GGAAGGCACG
    CGCAGTGCTG ACAACACTTT GGAGAGAAGG GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC
    CCGGAGAGGA GGCGAGAGCG GTCCCCGACC CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG
    CGGCGGTCTC TGGAGAGACT CTTGGATCAG AGAAGATCGC CCGAGCGCAA GAGAGGAAGC
    TCGCCCGAAA GGAGAGCCAA ATCCACCGAC CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG
    AGAGAGCGGT CCCTGGAGAA AAGGAACAAA GAGGACAGAG GCAGCCACCG GGAGAGGGAC
    GAGACGAGTC TAAGACAGGA CACCGGCCGG AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA
    AGACCTTACA AAGAATGCAG CACCGACCTC AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11009
    Clone ID Related Accession (Same CDS sequence) XM_024983160.1
    Accession Version XM_024983160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGGTCGG
    ATTATTGAGG GGAGCCCTGC TGACCGATGC GGCAAGCTGA AAGTGGGAGA CCGGATCTTG
    GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCACTCTG ACATTGTCAA CCTCATCAAG
    GAAGCGGGCA ACACAGTCAC CCTCCGCATC ATCCCTGGGG ATGAGTCTTC AAATGCCACT
    CTGCTGACCA ATGCGGAGAA GATAGCCACC ATCACCACCA CACACACCCC TTCGCAGCAA
    GGAGCCCAGG AGACCAGGAA TACCACCAAA CCAAAGCCAG AATCTCAGTT TGAATTCAAA
    GCACCCCAGG CAACACAGGA GCAAGATTTT TACACTGTGG AGCTGGAAAG AGGAGCCAAG
    GGATTTGGTT TCAGTCTTCG AGGAGGTCGA GAGTATAACA TGGACCTCTA CGTTCTGCGC
    TTAGCAGAGG ATGGTCCTGC CGAAAGGTGT GGGAAGATGA AGGTTGGTGA TGAAATTTTA
    GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GGGCTATAGA ACTGATTAAG
    AATGGTGGCC GCAGAGTGCG TCTGTTTCTG AAGCGGGGAG ACGGCTCGGT CCCAGAATAT
    GACCCCGGCA GCGACGGGAA CGGCCCCCCC ACCGGTCCAC AAGGTGTTCC GGAAATGAGG
    GCTGGGCCCC CGGACCGCCG ACAGCATCCA TCATTGGAGT CCAGCTACCC ACCCGATCTT
    CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGTGA GAGATCCCAA AGGCAGCAGA
    GAGTATAGCA GACAACCCAA TGAACATCAC ACGTGGAACG GAACTTCTAG AAAACCCGAC
    AGTGGGGCTT GCCGACCCAA GGACCGGGCA CCCGAGGGAC GAAGAGACGC CCAACCCGAG
    CGGATGGTGA CCAATGGCCC CAAAAGGAGG TCTCCGGAGA AGCGGAGGGA AGGCACGCGC
    AGTGCTGACA ACACTTTGGA GAGAAGGGAG AAGCAGGAGA AGAGGAAGGA GGTTTCCCCG
    GAGAGGAGGC GAGAGCGGTC CCCGACCCGC CGGAGGGATG GCTCTCCAGG CCGGCGGCGG
    CGGTCTCTGG AGAGACTCTT GGATCAGAGA AGATCGCCCG AGCGCAAGAG AGGAAGCTCG
    CCCGAAAGGA GAGCCAAATC CACCGACCGG AGGCGGACCA AGTCCCCCGA GCGGAGGAGA
    GAGCGGTCCC TGGAGAAAAG GAACAAAGAG GACAGAGGCA GCCACCGGGA GAGGGACGAG
    ACGAGTCTAA GACAGGACAC CGGCCGGAGT TCCAGACATC CCCCGGAGCA GCGGAGAAGA
    CCTTACAAAG AATGCAGCAC CGACCTCAGT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838928.1
    CDS618..5033
    Translation

    Target ORF information:

    RefSeq Version XM_024983160.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGGTCGG
    ATTATTGAGG GGAGCCCTGC TGACCGATGC GGCAAGCTGA AAGTGGGAGA CCGGATCTTG
    GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCACTCTG ACATTGTCAA CCTCATCAAG
    GAAGCGGGCA ACACAGTCAC CCTCCGCATC ATCCCTGGGG ATGAGTCTTC AAATGCCACT
    CTGCTGACCA ATGCGGAGAA GATAGCCACC ATCACCACCA CACACACCCC TTCGCAGCAA
    GGAGCCCAGG AGACCAGGAA TACCACCAAA CCAAAGCCAG AATCTCAGTT TGAATTCAAA
    GCACCCCAGG CAACACAGGA GCAAGATTTT TACACTGTGG AGCTGGAAAG AGGAGCCAAG
    GGATTTGGTT TCAGTCTTCG AGGAGGTCGA GAGTATAACA TGGACCTCTA CGTTCTGCGC
    TTAGCAGAGG ATGGTCCTGC CGAAAGGTGT GGGAAGATGA AGGTTGGTGA TGAAATTTTA
    GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GGGCTATAGA ACTGATTAAG
    AATGGTGGCC GCAGAGTGCG TCTGTTTCTG AAGCGGGGAG ACGGCTCGGT CCCAGAATAT
    GACCCCGGCA GCGACGGGAA CGGCCCCCCC ACCGGTCCAC AAGGTGTTCC GGAAATGAGG
    GCTGGGCCCC CGGACCGCCG ACAGCATCCA TCATTGGAGT CCAGCTACCC ACCCGATCTT
    CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGTGA GAGATCCCAA AGGCAGCAGA
    GAGTATAGCA GACAACCCAA TGAACATCAC ACGTGGAACG GAACTTCTAG AAAACCCGAC
    AGTGGGGCTT GCCGACCCAA GGACCGGGCA CCCGAGGGAC GAAGAGACGC CCAACCCGAG
    CGGATGGTGA CCAATGGCCC CAAAAGGAGG TCTCCGGAGA AGCGGAGGGA AGGCACGCGC
    AGTGCTGACA ACACTTTGGA GAGAAGGGAG AAGCAGGAGA AGAGGAAGGA GGTTTCCCCG
    GAGAGGAGGC GAGAGCGGTC CCCGACCCGC CGGAGGGATG GCTCTCCAGG CCGGCGGCGG
    CGGTCTCTGG AGAGACTCTT GGATCAGAGA AGATCGCCCG AGCGCAAGAG AGGAAGCTCG
    CCCGAAAGGA GAGCCAAATC CACCGACCGG AGGCGGACCA AGTCCCCCGA GCGGAGGAGA
    GAGCGGTCCC TGGAGAAAAG GAACAAAGAG GACAGAGGCA GCCACCGGGA GAGGGACGAG
    ACGAGTCTAA GACAGGACAC CGGCCGGAGT TCCAGACATC CCCCGGAGCA GCGGAGAAGA
    CCTTACAAAG AATGCAGCAC CGACCTCAGT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11010
    Clone ID Related Accession (Same CDS sequence) XM_024983161.1
    Accession Version XM_024983161.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACCTAT GTCACCTTCG CCTGCATCGG GATTGAGCAA GGGTGAAAGA
    GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCAGA TTCCAGATTA TCAAGAACAG
    GACATCTTCC TCTGGAGAAA AGAGACCGGA TTTGGATTTA GGATTCTGGG TGGCAATGAA
    CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACAGACGGC
    CGTCTGAGGT CTGGGGATGA ATTAATCTGT GTGGATGGGA CACCAGTCAT TGGAAAATCA
    CACCAGCTTG TGGTCCAGCT TATGCAACAA GCCGCCAAGC AAGGCCACGT CAATCTAACG
    GTGCGGCGTA AAGTGGTGTT TGCGGCCCCC AAAACCGAGA ACGAGGTACC CTCGCCAGCC
    TCATCCCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACGG AGGAGAAGCG GACCCCGCAG
    GGCAGCCAGA ACTCCCTGAA CACGGTGAGC TCAGGCAGTG GCAGCACCAG TGGCATCGGC
    AGTGGCGGCG GTGGCGGCAG CGGTGTGGTG AGCACCGTGG TCCAGCCCTA TGACGTGGAG
    ATCCGGCGCG GCGAGAACGA GGGCTTCGGC TTTGTCATCG TGTCATCGGT GAGCAGGCCC
    GAGGCCGGCA CGACGTTCGC AGGCAATGCA TGTGTGGCCA TGCCTCACAA AATAGGTCGG
    ATTATTGAGG GGAGCCCTGC TGACCGATGC GGCAAGCTGA AAGTGGGAGA CCGGATCTTG
    GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCACTCTG ACATTGTCAA CCTCATCAAG
    GAAGCGGGCA ACACAGTCAC CCTCCGCATC ATCCCTGGGG ATGAGTCTTC AAATGCCACT
    CTGCTGACCA ATGCGGAGAA GATAGCCACC ATCACCACCA CACACACCCC TTCGCAGCAA
    GGAGCCCAGG AGACCAGGAA TACCACCAAA CCAAAGCCAG AATCTCAGTT TGAATTCAAA
    GCACCCCAGG CAACACAGGA GCAAGATTTT TACACTGTGG AGCTGGAAAG AGGAGCCAAG
    GGATTTGGTT TCAGTCTTCG AGGAGGTCGA GAGTATAACA TGGACCTCTA CGTTCTGCGC
    TTAGCAGAGG ATGGTCCTGC CGAAAGGTGT GGGAAGATGA AGGTTGGTGA TGAAATTTTA
    GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GGGCTATAGA ACTGATTAAG
    AATGGTGGCC GCAGAGTGCG TCTGTTTCTG AAGCGGGGAG ACGGCTCGGT CCCAGAATAT
    GACCCCGGCA GCGACGGGAA CGGCCCCCCC ACCGGTCCAC AAGGTGTTCC GGAAATGAGG
    GCTGGGCCCC CGGACCGCCG ACAGCATCCA TCATTGGAGT CCAGCTACCC ACCCGATCTT
    CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGTGA GAGATCCCAA AGGCAGCAGA
    GAGTATAGCA GACAACCCAA TGAACATCAC ACGTGGAACG GAACTTCTAG AAAACCCGAC
    AGTGGGGCTT GCCGACCCAA GGACCGGGCA CCCGAGGGAC GAAGAGACGC CCAACCCGAG
    CGGATGGTGA CCAATGGCCC CAAAAGGAGG TCTCCGGAGA AGCGGAGGGA AGGCACGCGC
    AGTGCTGACA ACACTTTGGA GAGAAGGGAG AAGCAGGAGA AGAGGAAGGA GGTTTCCCCG
    GAGAGGAGGC GAGAGCGGTC CCCGACCCGC CGGAGGGATG GCTCTCCAGG CCGGCGGCGG
    CGGTCTCTGG AGAGACTCTT GGATCAGAGA AGATCGCCCG AGCGCAAGAG AGGAAGCTCG
    CCCGAAAGGA GAGCCAAATC CACCGACCGG AGGCGGACCA AGTCCCCCGA GCGGAGGAGA
    GAGCGGTCCC TGGAGAAAAG GAACAAAGAG GACAGAGGCA GCCACCGGGA GAGGGACGAG
    ACGAGTCTAA GACAGGACAC CGGCCGGAGT TCCAGACATC CCCCGGAGCA GCGGAGAAGA
    CCTTACAAAG AATGCAGCAC CGACCTCAGT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838929.1
    CDS618..5033
    Translation

    Target ORF information:

    RefSeq Version XM_024983161.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACCTAT GTCACCTTCG CCTGCATCGG GATTGAGCAA GGGTGAAAGA
    GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCAGA TTCCAGATTA TCAAGAACAG
    GACATCTTCC TCTGGAGAAA AGAGACCGGA TTTGGATTTA GGATTCTGGG TGGCAATGAA
    CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACAGACGGC
    CGTCTGAGGT CTGGGGATGA ATTAATCTGT GTGGATGGGA CACCAGTCAT TGGAAAATCA
    CACCAGCTTG TGGTCCAGCT TATGCAACAA GCCGCCAAGC AAGGCCACGT CAATCTAACG
    GTGCGGCGTA AAGTGGTGTT TGCGGCCCCC AAAACCGAGA ACGAGGTACC CTCGCCAGCC
    TCATCCCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACGG AGGAGAAGCG GACCCCGCAG
    GGCAGCCAGA ACTCCCTGAA CACGGTGAGC TCAGGCAGTG GCAGCACCAG TGGCATCGGC
    AGTGGCGGCG GTGGCGGCAG CGGTGTGGTG AGCACCGTGG TCCAGCCCTA TGACGTGGAG
    ATCCGGCGCG GCGAGAACGA GGGCTTCGGC TTTGTCATCG TGTCATCGGT GAGCAGGCCC
    GAGGCCGGCA CGACGTTCGC AGGCAATGCA TGTGTGGCCA TGCCTCACAA AATAGGTCGG
    ATTATTGAGG GGAGCCCTGC TGACCGATGC GGCAAGCTGA AAGTGGGAGA CCGGATCTTG
    GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCACTCTG ACATTGTCAA CCTCATCAAG
    GAAGCGGGCA ACACAGTCAC CCTCCGCATC ATCCCTGGGG ATGAGTCTTC AAATGCCACT
    CTGCTGACCA ATGCGGAGAA GATAGCCACC ATCACCACCA CACACACCCC TTCGCAGCAA
    GGAGCCCAGG AGACCAGGAA TACCACCAAA CCAAAGCCAG AATCTCAGTT TGAATTCAAA
    GCACCCCAGG CAACACAGGA GCAAGATTTT TACACTGTGG AGCTGGAAAG AGGAGCCAAG
    GGATTTGGTT TCAGTCTTCG AGGAGGTCGA GAGTATAACA TGGACCTCTA CGTTCTGCGC
    TTAGCAGAGG ATGGTCCTGC CGAAAGGTGT GGGAAGATGA AGGTTGGTGA TGAAATTTTA
    GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GGGCTATAGA ACTGATTAAG
    AATGGTGGCC GCAGAGTGCG TCTGTTTCTG AAGCGGGGAG ACGGCTCGGT CCCAGAATAT
    GACCCCGGCA GCGACGGGAA CGGCCCCCCC ACCGGTCCAC AAGGTGTTCC GGAAATGAGG
    GCTGGGCCCC CGGACCGCCG ACAGCATCCA TCATTGGAGT CCAGCTACCC ACCCGATCTT
    CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGTGA GAGATCCCAA AGGCAGCAGA
    GAGTATAGCA GACAACCCAA TGAACATCAC ACGTGGAACG GAACTTCTAG AAAACCCGAC
    AGTGGGGCTT GCCGACCCAA GGACCGGGCA CCCGAGGGAC GAAGAGACGC CCAACCCGAG
    CGGATGGTGA CCAATGGCCC CAAAAGGAGG TCTCCGGAGA AGCGGAGGGA AGGCACGCGC
    AGTGCTGACA ACACTTTGGA GAGAAGGGAG AAGCAGGAGA AGAGGAAGGA GGTTTCCCCG
    GAGAGGAGGC GAGAGCGGTC CCCGACCCGC CGGAGGGATG GCTCTCCAGG CCGGCGGCGG
    CGGTCTCTGG AGAGACTCTT GGATCAGAGA AGATCGCCCG AGCGCAAGAG AGGAAGCTCG
    CCCGAAAGGA GAGCCAAATC CACCGACCGG AGGCGGACCA AGTCCCCCGA GCGGAGGAGA
    GAGCGGTCCC TGGAGAAAAG GAACAAAGAG GACAGAGGCA GCCACCGGGA GAGGGACGAG
    ACGAGTCTAA GACAGGACAC CGGCCGGAGT TCCAGACATC CCCCGGAGCA GCGGAGAAGA
    CCTTACAAAG AATGCAGCAC CGACCTCAGT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11011
    Clone ID Related Accession (Same CDS sequence) XM_024983162.1
    Accession Version XM_024983162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACCTAT GTCACCTTCG CCTGCATCGG GATTGAGCAA GGGTGAAAGA
    GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCAGA TTCCAGATTA TCAAGAACAG
    GACATCTTCC TCTGGAGAAA AGAGACCGGA TTTGGATTTA GGATTCTGGG TGGCAATGAA
    CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACAGACGGC
    CGTCTGAGGT CTGGGGATGA ATTAATCTGT GTGGATGGGA CACCAGTCAT TGGAAAATCA
    CACCAGCTTG TGGTCCAGCT TATGCAACAA GCCGCCAAGC AAGGCCACGT CAATCTAACG
    GTGCGGCGTA AAGTGGTGTT TGCGGCCCCC AAAACCGAGA ACGAGGTACC CTCGCCAGCC
    TCATCCCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACGG AGGAGAAGCG GACCCCGCAG
    GGCAGCCAGA ACTCCCTGAA CACGGTGAGC TCAGGCAGTG GCAGCACCAG TGGCATCGGC
    AGTGGCGGCG GTGGCGGCAG CGGTGTGGTG AGCACCGTGG TCCAGCCCTA TGACGTGGAG
    ATCCGGCGCG GCGAGAACGA GGGCTTCGGC TTTGTCATCG TGTCATCGGT GAGCAGGCCC
    GAGGCCGGCA CGACGTTCGG CAATGCATGT GTGGCCATGC CTCACAAAAT AGGTCGGATT
    ATTGAGGGGA GCCCTGCTGA CCGATGCGGC AAGCTGAAAG TGGGAGACCG GATCTTGGCA
    GTAAATGGAT GTTCCATCAC CAACAAATCC CACTCTGACA TTGTCAACCT CATCAAGGAA
    GCGGGCAACA CAGTCACCCT CCGCATCATC CCTGGGGATG AGTCTTCAAA TGCCACTCTG
    CTGACCAATG CGGAGAAGAT AGCCACCATC ACCACCACAC ACACCCCTTC GCAGCAAGGA
    GCCCAGGAGA CCAGGAATAC CACCAAACCA AAGCCAGAAT CTCAGTTTGA ATTCAAAGCA
    CCCCAGGCAA CACAGGAGCA AGATTTTTAC ACTGTGGAGC TGGAAAGAGG AGCCAAGGGA
    TTTGGTTTCA GTCTTCGAGG AGGTCGAGAG TATAACATGG ACCTCTACGT TCTGCGCTTA
    GCAGAGGATG GTCCTGCCGA AAGGTGTGGG AAGATGAAGG TTGGTGATGA AATTTTAGAG
    ATCAATGGTG AGACCACCAA AAACATGAAG CATTCTCGGG CTATAGAACT GATTAAGAAT
    GGTGGCCGCA GAGTGCGTCT GTTTCTGAAG CGGGGAGACG GCTCGGTCCC AGAATATGAC
    CCCGGCAGCG ACGGGAACGG CCCCCCCACC GGTCCACAAG GTGTTCCGGA AATGAGGGCT
    GGGCCCCCGG ACCGCCGACA GCATCCATCA TTGGAGTCCA GCTACCCACC CGATCTTCAC
    AAATCATCAC CACATGGGGA AAAGCGGGCA CACGTGAGAG ATCCCAAAGG CAGCAGAGAG
    TATAGCAGAC AACCCAATGA ACATCACACG TGGAACGGAA CTTCTAGAAA ACCCGACAGT
    GGGGCTTGCC GACCCAAGGA CCGGGCACCC GAGGGACGAA GAGACGCCCA ACCCGAGCGG
    ATGGTGACCA ATGGCCCCAA AAGGAGGTCT CCGGAGAAGC GGAGGGAAGG CACGCGCAGT
    GCTGACAACA CTTTGGAGAG AAGGGAGAAG CAGGAGAAGA GGAAGGAGGT TTCCCCGGAG
    AGGAGGCGAG AGCGGTCCCC GACCCGCCGG AGGGATGGCT CTCCAGGCCG GCGGCGGCGG
    TCTCTGGAGA GACTCTTGGA TCAGAGAAGA TCGCCCGAGC GCAAGAGAGG AAGCTCGCCC
    GAAAGGAGAG CCAAATCCAC CGACCGGAGG CGGACCAAGT CCCCCGAGCG GAGGAGAGAG
    CGGTCCCTGG AGAAAAGGAA CAAAGAGGAC AGAGGCAGCC ACCGGGAGAG GGACGAGACG
    AGTCTAAGAC AGGACACCGG CCGGAGTTCC AGACATCCCC CGGAGCAGCG GAGAAGACCT
    TACAAAGAAT GCAGCACCGA CCTCAGTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838930.1
    CDS618..5030
    Translation

    Target ORF information:

    RefSeq Version XM_024983162.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACCTAT GTCACCTTCG CCTGCATCGG GATTGAGCAA GGGTGAAAGA
    GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCAGA TTCCAGATTA TCAAGAACAG
    GACATCTTCC TCTGGAGAAA AGAGACCGGA TTTGGATTTA GGATTCTGGG TGGCAATGAA
    CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACAGACGGC
    CGTCTGAGGT CTGGGGATGA ATTAATCTGT GTGGATGGGA CACCAGTCAT TGGAAAATCA
    CACCAGCTTG TGGTCCAGCT TATGCAACAA GCCGCCAAGC AAGGCCACGT CAATCTAACG
    GTGCGGCGTA AAGTGGTGTT TGCGGCCCCC AAAACCGAGA ACGAGGTACC CTCGCCAGCC
    TCATCCCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACGG AGGAGAAGCG GACCCCGCAG
    GGCAGCCAGA ACTCCCTGAA CACGGTGAGC TCAGGCAGTG GCAGCACCAG TGGCATCGGC
    AGTGGCGGCG GTGGCGGCAG CGGTGTGGTG AGCACCGTGG TCCAGCCCTA TGACGTGGAG
    ATCCGGCGCG GCGAGAACGA GGGCTTCGGC TTTGTCATCG TGTCATCGGT GAGCAGGCCC
    GAGGCCGGCA CGACGTTCGG CAATGCATGT GTGGCCATGC CTCACAAAAT AGGTCGGATT
    ATTGAGGGGA GCCCTGCTGA CCGATGCGGC AAGCTGAAAG TGGGAGACCG GATCTTGGCA
    GTAAATGGAT GTTCCATCAC CAACAAATCC CACTCTGACA TTGTCAACCT CATCAAGGAA
    GCGGGCAACA CAGTCACCCT CCGCATCATC CCTGGGGATG AGTCTTCAAA TGCCACTCTG
    CTGACCAATG CGGAGAAGAT AGCCACCATC ACCACCACAC ACACCCCTTC GCAGCAAGGA
    GCCCAGGAGA CCAGGAATAC CACCAAACCA AAGCCAGAAT CTCAGTTTGA ATTCAAAGCA
    CCCCAGGCAA CACAGGAGCA AGATTTTTAC ACTGTGGAGC TGGAAAGAGG AGCCAAGGGA
    TTTGGTTTCA GTCTTCGAGG AGGTCGAGAG TATAACATGG ACCTCTACGT TCTGCGCTTA
    GCAGAGGATG GTCCTGCCGA AAGGTGTGGG AAGATGAAGG TTGGTGATGA AATTTTAGAG
    ATCAATGGTG AGACCACCAA AAACATGAAG CATTCTCGGG CTATAGAACT GATTAAGAAT
    GGTGGCCGCA GAGTGCGTCT GTTTCTGAAG CGGGGAGACG GCTCGGTCCC AGAATATGAC
    CCCGGCAGCG ACGGGAACGG CCCCCCCACC GGTCCACAAG GTGTTCCGGA AATGAGGGCT
    GGGCCCCCGG ACCGCCGACA GCATCCATCA TTGGAGTCCA GCTACCCACC CGATCTTCAC
    AAATCATCAC CACATGGGGA AAAGCGGGCA CACGTGAGAG ATCCCAAAGG CAGCAGAGAG
    TATAGCAGAC AACCCAATGA ACATCACACG TGGAACGGAA CTTCTAGAAA ACCCGACAGT
    GGGGCTTGCC GACCCAAGGA CCGGGCACCC GAGGGACGAA GAGACGCCCA ACCCGAGCGG
    ATGGTGACCA ATGGCCCCAA AAGGAGGTCT CCGGAGAAGC GGAGGGAAGG CACGCGCAGT
    GCTGACAACA CTTTGGAGAG AAGGGAGAAG CAGGAGAAGA GGAAGGAGGT TTCCCCGGAG
    AGGAGGCGAG AGCGGTCCCC GACCCGCCGG AGGGATGGCT CTCCAGGCCG GCGGCGGCGG
    TCTCTGGAGA GACTCTTGGA TCAGAGAAGA TCGCCCGAGC GCAAGAGAGG AAGCTCGCCC
    GAAAGGAGAG CCAAATCCAC CGACCGGAGG CGGACCAAGT CCCCCGAGCG GAGGAGAGAG
    CGGTCCCTGG AGAAAAGGAA CAAAGAGGAC AGAGGCAGCC ACCGGGAGAG GGACGAGACG
    AGTCTAAGAC AGGACACCGG CCGGAGTTCC AGACATCCCC CGGAGCAGCG GAGAAGACCT
    TACAAAGAAT GCAGCACCGA CCTCAGTATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11012
    Clone ID Related Accession (Same CDS sequence) XM_024983163.1
    Accession Version XM_024983163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACTTCCA
    GATTATCAAG AACAGGACAT CTTCCTCTGG AGAAAAGAGA CCGGATTTGG ATTTAGGATT
    CTGGGTGGCA ATGAACCAGG GGAACCTATT TATATTGGTC ACATCGTACC ACTGGGTGCT
    GCTGATACAG ACGGCCGTCT GAGGTCTGGG GATGAATTAA TCTGTGTGGA TGGGACACCA
    GTCATTGGAA AATCACACCA GCTTGTGGTC CAGCTTATGC AACAAGCCGC CAAGCAAGGC
    CACGTCAATC TAACGGTGCG GCGTAAAGTG GTGTTTGCGG CCCCCAAAAC CGAGAACGAG
    GTACCCTCGC CAGCCTCATC CCATCACAGT AGCAACCAGC CGGCCTCGCT GACGGAGGAG
    AAGCGGACCC CGCAGGGCAG CCAGAACTCC CTGAACACGG TGAGCTCAGG CAGTGGCAGC
    ACCAGTGGCA TCGGCAGTGG CGGCGGTGGC GGCAGCGGTG TGGTGAGCAC CGTGGTCCAG
    CCCTATGACG TGGAGATCCG GCGCGGCGAG AACGAGGGCT TCGGCTTTGT CATCGTGTCA
    TCGGTGAGCA GGCCCGAGGC CGGCACGACG TTCGCAGGCA ATGCATGTGT GGCCATGCCT
    CACAAAATAG GTCGGATTAT TGAGGGGAGC CCTGCTGACC GATGCGGCAA GCTGAAAGTG
    GGAGACCGGA TCTTGGCAGT AAATGGATGT TCCATCACCA ACAAATCCCA CTCTGACATT
    GTCAACCTCA TCAAGGAAGC GGGCAACACA GTCACCCTCC GCATCATCCC TGGGGATGAG
    TCTTCAAATG CCACTCTGCT GACCAATGCG GAGAAGATAG CCACCATCAC CACCACACAC
    ACCCCTTCGC AGCAAGGAGC CCAGGAGACC AGGAATACCA CCAAACCAAA GCCAGAATCT
    CAGTTTGAAT TCAAAGCACC CCAGGCAACA CAGGAGCAAG ATTTTTACAC TGTGGAGCTG
    GAAAGAGGAG CCAAGGGATT TGGTTTCAGT CTTCGAGGAG GTCGAGAGTA TAACATGGAC
    CTCTACGTTC TGCGCTTAGC AGAGGATGGT CCTGCCGAAA GGTGTGGGAA GATGAAGGTT
    GGTGATGAAA TTTTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAGCA TTCTCGGGCT
    ATAGAACTGA TTAAGAATGG TGGCCGCAGA GTGCGTCTGT TTCTGAAGCG GGGAGACGGC
    TCGGTCCCAG AATATGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT
    GTTCCGGAAA TGAGGGCTGG GCCCCCGGAC CGCCGACAGC ATCCATCATT GGAGTCCAGC
    TACCCACCCG ATCTTCACAA ATCATCACCA CATGGGGAAA AGCGGGCACA CGTGAGAGAT
    CCCAAAGGCA GCAGAGAGTA TAGCAGACAA CCCAATGAAC ATCACACGTG GAACGGAACT
    TCTAGAAAAC CCGACAGTGG GGCTTGCCGA CCCAAGGACC GGGCACCCGA GGGACGAAGA
    GACGCCCAAC CCGAGCGGAT GGTGACCAAT GGCCCCAAAA GGAGGTCTCC GGAGAAGCGG
    AGGGAAGGCA CGCGCAGTGC TGACAACACT TTGGAGAGAA GGGAGAAGCA GGAGAAGAGG
    AAGGAGGTTT CCCCGGAGAG GAGGCGAGAG CGGTCCCCGA CCCGCCGGAG GGATGGCTCT
    CCAGGCCGGC GGCGGCGGTC TCTGGAGAGA CTCTTGGATC AGAGAAGATC GCCCGAGCGC
    AAGAGAGGAA GCTCGCCCGA AAGGAGAGCC AAATCCACCG ACCGGAGGCG GACCAAGTCC
    CCCGAGCGGA GGAGAGAGCG GTCCCTGGAG AAAAGGAACA AAGAGGACAG AGGCAGCCAC
    CGGGAGAGGG ACGAGACGAG TCTAAGACAG GACACCGGCC GGAGTTCCAG ACATCCCCCG
    GAGCAGCGGA GAAGACCTTA CAAAGAATGC AGCACCGACC TCAGTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838931.1
    CDS618..4988
    Translation

    Target ORF information:

    RefSeq Version XM_024983163.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACTTCCA
    GATTATCAAG AACAGGACAT CTTCCTCTGG AGAAAAGAGA CCGGATTTGG ATTTAGGATT
    CTGGGTGGCA ATGAACCAGG GGAACCTATT TATATTGGTC ACATCGTACC ACTGGGTGCT
    GCTGATACAG ACGGCCGTCT GAGGTCTGGG GATGAATTAA TCTGTGTGGA TGGGACACCA
    GTCATTGGAA AATCACACCA GCTTGTGGTC CAGCTTATGC AACAAGCCGC CAAGCAAGGC
    CACGTCAATC TAACGGTGCG GCGTAAAGTG GTGTTTGCGG CCCCCAAAAC CGAGAACGAG
    GTACCCTCGC CAGCCTCATC CCATCACAGT AGCAACCAGC CGGCCTCGCT GACGGAGGAG
    AAGCGGACCC CGCAGGGCAG CCAGAACTCC CTGAACACGG TGAGCTCAGG CAGTGGCAGC
    ACCAGTGGCA TCGGCAGTGG CGGCGGTGGC GGCAGCGGTG TGGTGAGCAC CGTGGTCCAG
    CCCTATGACG TGGAGATCCG GCGCGGCGAG AACGAGGGCT TCGGCTTTGT CATCGTGTCA
    TCGGTGAGCA GGCCCGAGGC CGGCACGACG TTCGCAGGCA ATGCATGTGT GGCCATGCCT
    CACAAAATAG GTCGGATTAT TGAGGGGAGC CCTGCTGACC GATGCGGCAA GCTGAAAGTG
    GGAGACCGGA TCTTGGCAGT AAATGGATGT TCCATCACCA ACAAATCCCA CTCTGACATT
    GTCAACCTCA TCAAGGAAGC GGGCAACACA GTCACCCTCC GCATCATCCC TGGGGATGAG
    TCTTCAAATG CCACTCTGCT GACCAATGCG GAGAAGATAG CCACCATCAC CACCACACAC
    ACCCCTTCGC AGCAAGGAGC CCAGGAGACC AGGAATACCA CCAAACCAAA GCCAGAATCT
    CAGTTTGAAT TCAAAGCACC CCAGGCAACA CAGGAGCAAG ATTTTTACAC TGTGGAGCTG
    GAAAGAGGAG CCAAGGGATT TGGTTTCAGT CTTCGAGGAG GTCGAGAGTA TAACATGGAC
    CTCTACGTTC TGCGCTTAGC AGAGGATGGT CCTGCCGAAA GGTGTGGGAA GATGAAGGTT
    GGTGATGAAA TTTTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAGCA TTCTCGGGCT
    ATAGAACTGA TTAAGAATGG TGGCCGCAGA GTGCGTCTGT TTCTGAAGCG GGGAGACGGC
    TCGGTCCCAG AATATGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT
    GTTCCGGAAA TGAGGGCTGG GCCCCCGGAC CGCCGACAGC ATCCATCATT GGAGTCCAGC
    TACCCACCCG ATCTTCACAA ATCATCACCA CATGGGGAAA AGCGGGCACA CGTGAGAGAT
    CCCAAAGGCA GCAGAGAGTA TAGCAGACAA CCCAATGAAC ATCACACGTG GAACGGAACT
    TCTAGAAAAC CCGACAGTGG GGCTTGCCGA CCCAAGGACC GGGCACCCGA GGGACGAAGA
    GACGCCCAAC CCGAGCGGAT GGTGACCAAT GGCCCCAAAA GGAGGTCTCC GGAGAAGCGG
    AGGGAAGGCA CGCGCAGTGC TGACAACACT TTGGAGAGAA GGGAGAAGCA GGAGAAGAGG
    AAGGAGGTTT CCCCGGAGAG GAGGCGAGAG CGGTCCCCGA CCCGCCGGAG GGATGGCTCT
    CCAGGCCGGC GGCGGCGGTC TCTGGAGAGA CTCTTGGATC AGAGAAGATC GCCCGAGCGC
    AAGAGAGGAA GCTCGCCCGA AAGGAGAGCC AAATCCACCG ACCGGAGGCG GACCAAGTCC
    CCCGAGCGGA GGAGAGAGCG GTCCCTGGAG AAAAGGAACA AAGAGGACAG AGGCAGCCAC
    CGGGAGAGGG ACGAGACGAG TCTAAGACAG GACACCGGCC GGAGTTCCAG ACATCCCCCG
    GAGCAGCGGA GAAGACCTTA CAAAGAATGC AGCACCGACC TCAGTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11013
    Clone ID Related Accession (Same CDS sequence) XM_024983164.1
    Accession Version XM_024983164.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACTTCCA
    GATTATCAAG AACAGGACAT CTTCCTCTGG AGAAAAGAGA CCGGATTTGG ATTTAGGATT
    CTGGGTGGCA ATGAACCAGG GGAACCTATT TATATTGGTC ACATCGTACC ACTGGGTGCT
    GCTGATACAG ACGGCCGTCT GAGGTCTGGG GATGAATTAA TCTGTGTGGA TGGGACACCA
    GTCATTGGAA AATCACACCA GCTTGTGGTC CAGCTTATGC AACAAGCCGC CAAGCAAGGC
    CACGTCAATC TAACGGTGCG GCGTAAAGTG GTGTTTGCGG CCCCCAAAAC CGAGAACGAG
    GTACCCTCGC CAGCCTCATC CCATCACAGT AGCAACCAGC CGGCCTCGCT GACGGAGGAG
    AAGCGGACCC CGCAGGGCAG CCAGAACTCC CTGAACACGG TGAGCTCAGG CAGTGGCAGC
    ACCAGTGGCA TCGGCAGTGG CGGCGGTGGC GGCAGCGGTG TGGTGAGCAC CGTGGTCCAG
    CCCTATGACG TGGAGATCCG GCGCGGCGAG AACGAGGGCT TCGGCTTTGT CATCGTGTCA
    TCGGTGAGCA GGCCCGAGGC CGGCACGACG TTCGGCAATG CATGTGTGGC CATGCCTCAC
    AAAATAGGTC GGATTATTGA GGGGAGCCCT GCTGACCGAT GCGGCAAGCT GAAAGTGGGA
    GACCGGATCT TGGCAGTAAA TGGATGTTCC ATCACCAACA AATCCCACTC TGACATTGTC
    AACCTCATCA AGGAAGCGGG CAACACAGTC ACCCTCCGCA TCATCCCTGG GGATGAGTCT
    TCAAATGCCA CTCTGCTGAC CAATGCGGAG AAGATAGCCA CCATCACCAC CACACACACC
    CCTTCGCAGC AAGGAGCCCA GGAGACCAGG AATACCACCA AACCAAAGCC AGAATCTCAG
    TTTGAATTCA AAGCACCCCA GGCAACACAG GAGCAAGATT TTTACACTGT GGAGCTGGAA
    AGAGGAGCCA AGGGATTTGG TTTCAGTCTT CGAGGAGGTC GAGAGTATAA CATGGACCTC
    TACGTTCTGC GCTTAGCAGA GGATGGTCCT GCCGAAAGGT GTGGGAAGAT GAAGGTTGGT
    GATGAAATTT TAGAGATCAA TGGTGAGACC ACCAAAAACA TGAAGCATTC TCGGGCTATA
    GAACTGATTA AGAATGGTGG CCGCAGAGTG CGTCTGTTTC TGAAGCGGGG AGACGGCTCG
    GTCCCAGAAT ATGACCCCGG CAGCGACGGG AACGGCCCCC CCACCGGTCC ACAAGGTGTT
    CCGGAAATGA GGGCTGGGCC CCCGGACCGC CGACAGCATC CATCATTGGA GTCCAGCTAC
    CCACCCGATC TTCACAAATC ATCACCACAT GGGGAAAAGC GGGCACACGT GAGAGATCCC
    AAAGGCAGCA GAGAGTATAG CAGACAACCC AATGAACATC ACACGTGGAA CGGAACTTCT
    AGAAAACCCG ACAGTGGGGC TTGCCGACCC AAGGACCGGG CACCCGAGGG ACGAAGAGAC
    GCCCAACCCG AGCGGATGGT GACCAATGGC CCCAAAAGGA GGTCTCCGGA GAAGCGGAGG
    GAAGGCACGC GCAGTGCTGA CAACACTTTG GAGAGAAGGG AGAAGCAGGA GAAGAGGAAG
    GAGGTTTCCC CGGAGAGGAG GCGAGAGCGG TCCCCGACCC GCCGGAGGGA TGGCTCTCCA
    GGCCGGCGGC GGCGGTCTCT GGAGAGACTC TTGGATCAGA GAAGATCGCC CGAGCGCAAG
    AGAGGAAGCT CGCCCGAAAG GAGAGCCAAA TCCACCGACC GGAGGCGGAC CAAGTCCCCC
    GAGCGGAGGA GAGAGCGGTC CCTGGAGAAA AGGAACAAAG AGGACAGAGG CAGCCACCGG
    GAGAGGGACG AGACGAGTCT AAGACAGGAC ACCGGCCGGA GTTCCAGACA TCCCCCGGAG
    CAGCGGAGAA GACCTTACAA AGAATGCAGC ACCGACCTCA GTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838932.1
    CDS618..4985
    Translation

    Target ORF information:

    RefSeq Version XM_024983164.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983164.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACTTCCA
    GATTATCAAG AACAGGACAT CTTCCTCTGG AGAAAAGAGA CCGGATTTGG ATTTAGGATT
    CTGGGTGGCA ATGAACCAGG GGAACCTATT TATATTGGTC ACATCGTACC ACTGGGTGCT
    GCTGATACAG ACGGCCGTCT GAGGTCTGGG GATGAATTAA TCTGTGTGGA TGGGACACCA
    GTCATTGGAA AATCACACCA GCTTGTGGTC CAGCTTATGC AACAAGCCGC CAAGCAAGGC
    CACGTCAATC TAACGGTGCG GCGTAAAGTG GTGTTTGCGG CCCCCAAAAC CGAGAACGAG
    GTACCCTCGC CAGCCTCATC CCATCACAGT AGCAACCAGC CGGCCTCGCT GACGGAGGAG
    AAGCGGACCC CGCAGGGCAG CCAGAACTCC CTGAACACGG TGAGCTCAGG CAGTGGCAGC
    ACCAGTGGCA TCGGCAGTGG CGGCGGTGGC GGCAGCGGTG TGGTGAGCAC CGTGGTCCAG
    CCCTATGACG TGGAGATCCG GCGCGGCGAG AACGAGGGCT TCGGCTTTGT CATCGTGTCA
    TCGGTGAGCA GGCCCGAGGC CGGCACGACG TTCGGCAATG CATGTGTGGC CATGCCTCAC
    AAAATAGGTC GGATTATTGA GGGGAGCCCT GCTGACCGAT GCGGCAAGCT GAAAGTGGGA
    GACCGGATCT TGGCAGTAAA TGGATGTTCC ATCACCAACA AATCCCACTC TGACATTGTC
    AACCTCATCA AGGAAGCGGG CAACACAGTC ACCCTCCGCA TCATCCCTGG GGATGAGTCT
    TCAAATGCCA CTCTGCTGAC CAATGCGGAG AAGATAGCCA CCATCACCAC CACACACACC
    CCTTCGCAGC AAGGAGCCCA GGAGACCAGG AATACCACCA AACCAAAGCC AGAATCTCAG
    TTTGAATTCA AAGCACCCCA GGCAACACAG GAGCAAGATT TTTACACTGT GGAGCTGGAA
    AGAGGAGCCA AGGGATTTGG TTTCAGTCTT CGAGGAGGTC GAGAGTATAA CATGGACCTC
    TACGTTCTGC GCTTAGCAGA GGATGGTCCT GCCGAAAGGT GTGGGAAGAT GAAGGTTGGT
    GATGAAATTT TAGAGATCAA TGGTGAGACC ACCAAAAACA TGAAGCATTC TCGGGCTATA
    GAACTGATTA AGAATGGTGG CCGCAGAGTG CGTCTGTTTC TGAAGCGGGG AGACGGCTCG
    GTCCCAGAAT ATGACCCCGG CAGCGACGGG AACGGCCCCC CCACCGGTCC ACAAGGTGTT
    CCGGAAATGA GGGCTGGGCC CCCGGACCGC CGACAGCATC CATCATTGGA GTCCAGCTAC
    CCACCCGATC TTCACAAATC ATCACCACAT GGGGAAAAGC GGGCACACGT GAGAGATCCC
    AAAGGCAGCA GAGAGTATAG CAGACAACCC AATGAACATC ACACGTGGAA CGGAACTTCT
    AGAAAACCCG ACAGTGGGGC TTGCCGACCC AAGGACCGGG CACCCGAGGG ACGAAGAGAC
    GCCCAACCCG AGCGGATGGT GACCAATGGC CCCAAAAGGA GGTCTCCGGA GAAGCGGAGG
    GAAGGCACGC GCAGTGCTGA CAACACTTTG GAGAGAAGGG AGAAGCAGGA GAAGAGGAAG
    GAGGTTTCCC CGGAGAGGAG GCGAGAGCGG TCCCCGACCC GCCGGAGGGA TGGCTCTCCA
    GGCCGGCGGC GGCGGTCTCT GGAGAGACTC TTGGATCAGA GAAGATCGCC CGAGCGCAAG
    AGAGGAAGCT CGCCCGAAAG GAGAGCCAAA TCCACCGACC GGAGGCGGAC CAAGTCCCCC
    GAGCGGAGGA GAGAGCGGTC CCTGGAGAAA AGGAACAAAG AGGACAGAGG CAGCCACCGG
    GAGAGGGACG AGACGAGTCT AAGACAGGAC ACCGGCCGGA GTTCCAGACA TCCCCCGGAG
    CAGCGGAGAA GACCTTACAA AGAATGCAGC ACCGACCTCA GTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11014
    Clone ID Related Accession (Same CDS sequence) XM_024983170.1
    Accession Version XM_024983170.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGAG
    TCTTCAAATG CCACTCTGCT GACCAATGCG GAGAAGATAG CCACCATCAC CACCACACAC
    ACCCCTTCGC AGCAAGGAGC CCAGGAGACC AGGAATACCA CCAAACCAAA GCCAGAATCT
    CAGTTTGAAT TCAAAGCACC CCAGGCAACA CAGGAGCAAG ATTTTTACAC TGTGGAGCTG
    GAAAGAGGAG CCAAGGGATT TGGTTTCAGT CTTCGAGGAG GTCGAGAGTA TAACATGGAC
    CTCTACGTTC TGCGCTTAGC AGAGGATGGT CCTGCCGAAA GGTGTGGGAA GATGAAGGTT
    GGTGATGAAA TTTTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAGCA TTCTCGGGCT
    ATAGAACTGA TTAAGAATGG TGGCCGCAGA GTGCGTCTGT TTCTGAAGCG GGGAGACGGC
    TCGGTCCCAG AATATGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT
    GTTCCGGAAA TGAGGGCTGG GCCCCCGGAC CGCCGACAGC ATCCATCATT GGAGTCCAGC
    TACCCACCCG ATCTTCACAA ATCATCACCA CATGGGGAAA AGCGGGCACA CGTGAGAGAT
    CCCAAAGGCA GCAGAGAGTA TAGCAGACAA CCCAATGAAC ATCACACGTG GAACGGAACT
    TCTAGAAAAC CCGACAGTGG GGCTTGCCGA CCCAAGGACC GGGCACCCGA GGGACGAAGA
    GACGCCCAAC CCGAGCGGAT GGTGACCAAT GGCCCCAAAA GGAGGTCTCC GGAGAAGCGG
    AGGGAAGGCA CGCGCAGTGC TGACAACACT TTGGAGAGAA GGGAGAAGCA GGAGAAGAGG
    AAGGAGGTTT CCCCGGAGAG GAGGCGAGAG CGGTCCCCGA CCCGCCGGAG GGATGGCTCT
    CCAGGCCGGC GGCGGCGGTC TCTGGAGAGA CTCTTGGATC AGAGAAGATC GCCCGAGCGC
    AAGAGAGGAA GCTCGCCCGA AAGGAGAGCC AAATCCACCG ACCGGAGGCG GACCAAGTCC
    CCCGAGCGGA GGAGAGAGCG GTCCCTGGAG AAAAGGAACA AAGAGGACAG AGGCAGCCAC
    CGGGAGAGGG ACGAGACGAG TCTAAGACAG GACACCGGCC GGAGTTCCAG ACATCCCCCG
    GAGCAGCGGA GAAGACCTTA CAAAGAATGC AGCACCGACC TCAGTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838938.1
    CDS618..4868
    Translation

    Target ORF information:

    RefSeq Version XM_024983170.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGAG
    TCTTCAAATG CCACTCTGCT GACCAATGCG GAGAAGATAG CCACCATCAC CACCACACAC
    ACCCCTTCGC AGCAAGGAGC CCAGGAGACC AGGAATACCA CCAAACCAAA GCCAGAATCT
    CAGTTTGAAT TCAAAGCACC CCAGGCAACA CAGGAGCAAG ATTTTTACAC TGTGGAGCTG
    GAAAGAGGAG CCAAGGGATT TGGTTTCAGT CTTCGAGGAG GTCGAGAGTA TAACATGGAC
    CTCTACGTTC TGCGCTTAGC AGAGGATGGT CCTGCCGAAA GGTGTGGGAA GATGAAGGTT
    GGTGATGAAA TTTTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAGCA TTCTCGGGCT
    ATAGAACTGA TTAAGAATGG TGGCCGCAGA GTGCGTCTGT TTCTGAAGCG GGGAGACGGC
    TCGGTCCCAG AATATGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT
    GTTCCGGAAA TGAGGGCTGG GCCCCCGGAC CGCCGACAGC ATCCATCATT GGAGTCCAGC
    TACCCACCCG ATCTTCACAA ATCATCACCA CATGGGGAAA AGCGGGCACA CGTGAGAGAT
    CCCAAAGGCA GCAGAGAGTA TAGCAGACAA CCCAATGAAC ATCACACGTG GAACGGAACT
    TCTAGAAAAC CCGACAGTGG GGCTTGCCGA CCCAAGGACC GGGCACCCGA GGGACGAAGA
    GACGCCCAAC CCGAGCGGAT GGTGACCAAT GGCCCCAAAA GGAGGTCTCC GGAGAAGCGG
    AGGGAAGGCA CGCGCAGTGC TGACAACACT TTGGAGAGAA GGGAGAAGCA GGAGAAGAGG
    AAGGAGGTTT CCCCGGAGAG GAGGCGAGAG CGGTCCCCGA CCCGCCGGAG GGATGGCTCT
    CCAGGCCGGC GGCGGCGGTC TCTGGAGAGA CTCTTGGATC AGAGAAGATC GCCCGAGCGC
    AAGAGAGGAA GCTCGCCCGA AAGGAGAGCC AAATCCACCG ACCGGAGGCG GACCAAGTCC
    CCCGAGCGGA GGAGAGAGCG GTCCCTGGAG AAAAGGAACA AAGAGGACAG AGGCAGCCAC
    CGGGAGAGGG ACGAGACGAG TCTAAGACAG GACACCGGCC GGAGTTCCAG ACATCCCCCG
    GAGCAGCGGA GAAGACCTTA CAAAGAATGC AGCACCGACC TCAGTATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11015
    Clone ID Related Accession (Same CDS sequence) XM_024983171.1
    Accession Version XM_024983171.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGCA
    GGCAATGCAT GTGTGGCCAT GCCTCACAAA ATAGGTCGGA TTATTGAGGG GAGCCCTGCT
    GACCGATGCG GCAAGCTGAA AGTGGGAGAC CGGATCTTGG CAGTAAATGG ATGTTCCATC
    ACCAACAAAT CCCACTCTGA CATTGTCAAC CTCATCAAGG AAGCGGGCAA CACAGTCACC
    CTCCGCATCA TCCCTGGGGA TGAGTCTTCA AATGCCACTC TGCTGACCAA TGCGGAGAAG
    ATAGCCACCA TCACCACCAC ACACACCCCT TCGCAGCAAG GAGCCCAGGA GACCAGGAAT
    ACCACCAAAC CAAAGCCAGA ATCTCAGTTT GAATTCAAAG CACCCCAGGC AACACAGGAG
    CAAGATTTTT ACACTGTGGA GCTGGAAAGA GGAGCCAAGG GATTTGGTTT CAGTCTTCGA
    GGAGGTCGAG AGTATAACAT GGACCTCTAC GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC
    GAAAGGTGTG GGAAGATGAA GGTTGGTGAT GAAATTTTAG AGATCAATGG TGAGACCACC
    AAAAACATGA AGCATTCTCG GGCTATAGAA CTGATTAAGA ATGGTGGCCG CAGAGTGCGT
    CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC CCAGAATATG CGATGATACC TCCTAATATC
    GCTGCATGTA TGAGAAATGA AAAGCTCGGG GAGGCTTGCT TCTACCTTAT GGGCCATAAT
    CAAACTACGA CCCCGGCAGC GACGGGAACG GCCCCCCCAC CGGTCCACAA GGTGTTCCGG
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838939.1
    CDS619..4464
    Translation

    Target ORF information:

    RefSeq Version XM_024983171.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983171.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGCA
    GGCAATGCAT GTGTGGCCAT GCCTCACAAA ATAGGTCGGA TTATTGAGGG GAGCCCTGCT
    GACCGATGCG GCAAGCTGAA AGTGGGAGAC CGGATCTTGG CAGTAAATGG ATGTTCCATC
    ACCAACAAAT CCCACTCTGA CATTGTCAAC CTCATCAAGG AAGCGGGCAA CACAGTCACC
    CTCCGCATCA TCCCTGGGGA TGAGTCTTCA AATGCCACTC TGCTGACCAA TGCGGAGAAG
    ATAGCCACCA TCACCACCAC ACACACCCCT TCGCAGCAAG GAGCCCAGGA GACCAGGAAT
    ACCACCAAAC CAAAGCCAGA ATCTCAGTTT GAATTCAAAG CACCCCAGGC AACACAGGAG
    CAAGATTTTT ACACTGTGGA GCTGGAAAGA GGAGCCAAGG GATTTGGTTT CAGTCTTCGA
    GGAGGTCGAG AGTATAACAT GGACCTCTAC GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC
    GAAAGGTGTG GGAAGATGAA GGTTGGTGAT GAAATTTTAG AGATCAATGG TGAGACCACC
    AAAAACATGA AGCATTCTCG GGCTATAGAA CTGATTAAGA ATGGTGGCCG CAGAGTGCGT
    CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC CCAGAATATG CGATGATACC TCCTAATATC
    GCTGCATGTA TGAGAAATGA AAAGCTCGGG GAGGCTTGCT TCTACCTTAT GGGCCATAAT
    CAAACTACGA CCCCGGCAGC GACGGGAACG GCCCCCCCAC CGGTCCACAA GGTGTTCCGG
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11016
    Clone ID Related Accession (Same CDS sequence) XM_024983173.1
    Accession Version XM_024983173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGCGA TGATACCTCC TAATATCGCT
    GCATGTATGA GAAATGAAAA GCTCGGGGAG GCTTGCTTCT ACCTTATGGG CCATAATCAA
    ACTACGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT GTTCCGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838941.1
    CDS619..4461
    Translation

    Target ORF information:

    RefSeq Version XM_024983173.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGCGA TGATACCTCC TAATATCGCT
    GCATGTATGA GAAATGAAAA GCTCGGGGAG GCTTGCTTCT ACCTTATGGG CCATAATCAA
    ACTACGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT GTTCCGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11017
    Clone ID Related Accession (Same CDS sequence) XM_024983172.1
    Accession Version XM_024983172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGCGA TGATACCTCC TAATATCGCT
    GCATGTATGA GAAATGAAAA GCTCGGGGAG GCTTGCTTCT ACCTTATGGG CCATAATCAA
    ACTACGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT GTTCCGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838940.1
    CDS619..4461
    Translation

    Target ORF information:

    RefSeq Version XM_024983172.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGCGA TGATACCTCC TAATATCGCT
    GCATGTATGA GAAATGAAAA GCTCGGGGAG GCTTGCTTCT ACCTTATGGG CCATAATCAA
    ACTACGACCC CGGCAGCGAC GGGAACGGCC CCCCCACCGG TCCACAAGGT GTTCCGGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11018
    Clone ID Related Accession (Same CDS sequence) XM_002696963.6
    Accession Version XM_002696963.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696963.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACTTCCAGAT
    TATCAAGAAC AGGACATCTT CCTCTGGAGA AAAGAGACCG GATTTGGATT TAGGATTCTG
    GGTGGCAATG AACCAGGGGA ACCTATTTAT ATTGGTCACA TCGTACCACT GGGTGCTGCT
    GATACAGACG GCCGTCTGAG GTCTGGGGAT GAATTAATCT GTGTGGATGG GACACCAGTC
    ATTGGAAAAT CACACCAGCT TGTGGTCCAG CTTATGCAAC AAGCCGCCAA GCAAGGCCAC
    GTCAATCTAA CGGTGCGGCG TAAAGTGGTG TTTGCGGCCC CCAAAACCGA GAACGAGGTA
    CCCTCGCCAG CCTCATCCCA TCACAGTAGC AACCAGCCGG CCTCGCTGAC GGAGGAGAAG
    CGGACCCCGC AGGGCAGCCA GAACTCCCTG AACACGGTGA GCTCAGGCAG TGGCAGCACC
    AGTGGCATCG GCAGTGGCGG CGGTGGCGGC AGCGGTGTGG TGAGCACCGT GGTCCAGCCC
    TATGACGTGG AGATCCGGCG CGGCGAGAAC GAGGGCTTCG GCTTTGTCAT CGTGTCATCG
    GTGAGCAGGC CCGAGGCCGG CACGACGTTC GGCAATGCAT GTGTGGCCAT GCCTCACAAA
    ATAGGTCGGA TTATTGAGGG GAGCCCTGCT GACCGATGCG GCAAGCTGAA AGTGGGAGAC
    CGGATCTTGG CAGTAAATGG ATGTTCCATC ACCAACAAAT CCCACTCTGA CATTGTCAAC
    CTCATCAAGG AAGCGGGCAA CACAGTCACC CTCCGCATCA TCCCTGGGGA TGAGTCTTCA
    AATGCCACTC TGCTGACCAA TGCGGAGAAG ATAGCCACCA TCACCACCAC ACACACCCCT
    TCGCAGCAAG GAGCCCAGGA GACCAGGAAT ACCACCAAAC CAAAGCCAGA ATCTCAGTTT
    GAATTCAAAG CACCCCAGGC AACACAGGAG CAAGATTTTT ACACTGTGGA GCTGGAAAGA
    GGAGCCAAGG GATTTGGTTT CAGTCTTCGA GGAGGTCGAG AGTATAACAT GGACCTCTAC
    GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC GAAAGGTGTG GGAAGATGAA GGTTGGTGAT
    GAAATTTTAG AGATCAATGG TGAGACCACC AAAAACATGA AGCATTCTCG GGCTATAGAA
    CTGATTAAGA ATGGTGGCCG CAGAGTGCGT CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC
    CCAGAATATG ACCCCGGCAG CGACGGGAAC GGCCCCCCCA CCGGTCCACA AGGTGTTCCG
    GAAATGAGGG CTGGGCCCCC GGACCGCCGA CAGCATCCAT CATTGGAGTC CAGCTACCCA
    CCCGATCTTC ACAAATCATC ACCACATGGG GAAAAGCGGG CACACGTGAG AGATCCCAAA
    GGCAGCAGAG AGTATAGCAG ACAACCCAAT GAACATCACA CGTGGAACGG AACTTCTAGA
    AAACCCGACA GTGGGGCTTG CCGACCCAAG GACCGGGCAC CCGAGGGACG AAGAGACGCC
    CAACCCGAGC GGATGGTGAC CAATGGCCCC AAAAGGAGGT CTCCGGAGAA GCGGAGGGAA
    GGCACGCGCA GTGCTGACAA CACTTTGGAG AGAAGGGAGA AGCAGGAGAA GAGGAAGGAG
    GTTTCCCCGG AGAGGAGGCG AGAGCGGTCC CCGACCCGCC GGAGGGATGG CTCTCCAGGC
    CGGCGGCGGC GGTCTCTGGA GAGACTCTTG GATCAGAGAA GATCGCCCGA GCGCAAGAGA
    GGAAGCTCGC CCGAAAGGAG AGCCAAATCC ACCGACCGGA GGCGGACCAA GTCCCCCGAG
    CGGAGGAGAG AGCGGTCCCT GGAGAAAAGG AACAAAGAGG ACAGAGGCAG CCACCGGGAG
    AGGGACGAGA CGAGTCTAAG ACAGGACACC GGCCGGAGTT CCAGACATCC CCCGGAGCAG
    CGGAGAAGAC CTTACAAAGA ATGCAGCACC GACCTCAGTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697009.2
    CDS618..4982
    Translation

    Target ORF information:

    RefSeq Version XM_002696963.6
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696963.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACTTCCAGAT
    TATCAAGAAC AGGACATCTT CCTCTGGAGA AAAGAGACCG GATTTGGATT TAGGATTCTG
    GGTGGCAATG AACCAGGGGA ACCTATTTAT ATTGGTCACA TCGTACCACT GGGTGCTGCT
    GATACAGACG GCCGTCTGAG GTCTGGGGAT GAATTAATCT GTGTGGATGG GACACCAGTC
    ATTGGAAAAT CACACCAGCT TGTGGTCCAG CTTATGCAAC AAGCCGCCAA GCAAGGCCAC
    GTCAATCTAA CGGTGCGGCG TAAAGTGGTG TTTGCGGCCC CCAAAACCGA GAACGAGGTA
    CCCTCGCCAG CCTCATCCCA TCACAGTAGC AACCAGCCGG CCTCGCTGAC GGAGGAGAAG
    CGGACCCCGC AGGGCAGCCA GAACTCCCTG AACACGGTGA GCTCAGGCAG TGGCAGCACC
    AGTGGCATCG GCAGTGGCGG CGGTGGCGGC AGCGGTGTGG TGAGCACCGT GGTCCAGCCC
    TATGACGTGG AGATCCGGCG CGGCGAGAAC GAGGGCTTCG GCTTTGTCAT CGTGTCATCG
    GTGAGCAGGC CCGAGGCCGG CACGACGTTC GGCAATGCAT GTGTGGCCAT GCCTCACAAA
    ATAGGTCGGA TTATTGAGGG GAGCCCTGCT GACCGATGCG GCAAGCTGAA AGTGGGAGAC
    CGGATCTTGG CAGTAAATGG ATGTTCCATC ACCAACAAAT CCCACTCTGA CATTGTCAAC
    CTCATCAAGG AAGCGGGCAA CACAGTCACC CTCCGCATCA TCCCTGGGGA TGAGTCTTCA
    AATGCCACTC TGCTGACCAA TGCGGAGAAG ATAGCCACCA TCACCACCAC ACACACCCCT
    TCGCAGCAAG GAGCCCAGGA GACCAGGAAT ACCACCAAAC CAAAGCCAGA ATCTCAGTTT
    GAATTCAAAG CACCCCAGGC AACACAGGAG CAAGATTTTT ACACTGTGGA GCTGGAAAGA
    GGAGCCAAGG GATTTGGTTT CAGTCTTCGA GGAGGTCGAG AGTATAACAT GGACCTCTAC
    GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC GAAAGGTGTG GGAAGATGAA GGTTGGTGAT
    GAAATTTTAG AGATCAATGG TGAGACCACC AAAAACATGA AGCATTCTCG GGCTATAGAA
    CTGATTAAGA ATGGTGGCCG CAGAGTGCGT CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC
    CCAGAATATG ACCCCGGCAG CGACGGGAAC GGCCCCCCCA CCGGTCCACA AGGTGTTCCG
    GAAATGAGGG CTGGGCCCCC GGACCGCCGA CAGCATCCAT CATTGGAGTC CAGCTACCCA
    CCCGATCTTC ACAAATCATC ACCACATGGG GAAAAGCGGG CACACGTGAG AGATCCCAAA
    GGCAGCAGAG AGTATAGCAG ACAACCCAAT GAACATCACA CGTGGAACGG AACTTCTAGA
    AAACCCGACA GTGGGGCTTG CCGACCCAAG GACCGGGCAC CCGAGGGACG AAGAGACGCC
    CAACCCGAGC GGATGGTGAC CAATGGCCCC AAAAGGAGGT CTCCGGAGAA GCGGAGGGAA
    GGCACGCGCA GTGCTGACAA CACTTTGGAG AGAAGGGAGA AGCAGGAGAA GAGGAAGGAG
    GTTTCCCCGG AGAGGAGGCG AGAGCGGTCC CCGACCCGCC GGAGGGATGG CTCTCCAGGC
    CGGCGGCGGC GGTCTCTGGA GAGACTCTTG GATCAGAGAA GATCGCCCGA GCGCAAGAGA
    GGAAGCTCGC CCGAAAGGAG AGCCAAATCC ACCGACCGGA GGCGGACCAA GTCCCCCGAG
    CGGAGGAGAG AGCGGTCCCT GGAGAAAAGG AACAAAGAGG ACAGAGGCAG CCACCGGGAG
    AGGGACGAGA CGAGTCTAAG ACAGGACACC GGCCGGAGTT CCAGACATCC CCCGGAGCAG
    CGGAGAAGAC CTTACAAAGA ATGCAGCACC GACCTCAGTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11019
    Clone ID Related Accession (Same CDS sequence) XM_024983178.1
    Accession Version XM_024983178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGGCAAT
    GCATGTGTGG CCATGCCTCA CAAAATAGGT CGGATTATTG AGGGGAGCCC TGCTGACCGA
    TGCGGCAAGC TGAAAGTGGG AGACCGGATC TTGGCAGTAA ATGGATGTTC CATCACCAAC
    AAATCCCACT CTGACATTGT CAACCTCATC AAGGAAGCGG GCAACACAGT CACCCTCCGC
    ATCATCCCTG GGGATGAGTC TTCAAATGCC ACTCTGCTGA CCAATGCGGA GAAGATAGCC
    ACCATCACCA CCACACACAC CCCTTCGCAG CAAGGAGCCC AGGAGACCAG GAATACCACC
    AAACCAAAGC CAGAATCTCA GTTTGAATTC AAAGCACCCC AGGCAACACA GGAGCAAGAT
    TTTTACACTG TGGAGCTGGA AAGAGGAGCC AAGGGATTTG GTTTCAGTCT TCGAGGAGGT
    CGAGAGTATA ACATGGACCT CTACGTTCTG CGCTTAGCAG AGGATGGTCC TGCCGAAAGG
    TGTGGGAAGA TGAAGGTTGG TGATGAAATT TTAGAGATCA ATGGTGAGAC CACCAAAAAC
    ATGAAGCATT CTCGGGCTAT AGAACTGATT AAGAATGGTG GCCGCAGAGT GCGTCTGTTT
    CTGAAGCGGG GAGACGGCTC GGTCCCAGAA TATGCGATGA TACCTCCTAA TATCGCTGCA
    TGTATGAGAA ATGAAAAGCT CGGGGAGGCT TGCTTCTACC TTATGGGCCA TAATCAAACT
    ACGACCCCGG CAGCGACGGG AACGGCCCCC CCACCGGTCC ACAAGGTGTT CCGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838946.1
    CDS619..4458
    Translation

    Target ORF information:

    RefSeq Version XM_024983178.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGGCAAT
    GCATGTGTGG CCATGCCTCA CAAAATAGGT CGGATTATTG AGGGGAGCCC TGCTGACCGA
    TGCGGCAAGC TGAAAGTGGG AGACCGGATC TTGGCAGTAA ATGGATGTTC CATCACCAAC
    AAATCCCACT CTGACATTGT CAACCTCATC AAGGAAGCGG GCAACACAGT CACCCTCCGC
    ATCATCCCTG GGGATGAGTC TTCAAATGCC ACTCTGCTGA CCAATGCGGA GAAGATAGCC
    ACCATCACCA CCACACACAC CCCTTCGCAG CAAGGAGCCC AGGAGACCAG GAATACCACC
    AAACCAAAGC CAGAATCTCA GTTTGAATTC AAAGCACCCC AGGCAACACA GGAGCAAGAT
    TTTTACACTG TGGAGCTGGA AAGAGGAGCC AAGGGATTTG GTTTCAGTCT TCGAGGAGGT
    CGAGAGTATA ACATGGACCT CTACGTTCTG CGCTTAGCAG AGGATGGTCC TGCCGAAAGG
    TGTGGGAAGA TGAAGGTTGG TGATGAAATT TTAGAGATCA ATGGTGAGAC CACCAAAAAC
    ATGAAGCATT CTCGGGCTAT AGAACTGATT AAGAATGGTG GCCGCAGAGT GCGTCTGTTT
    CTGAAGCGGG GAGACGGCTC GGTCCCAGAA TATGCGATGA TACCTCCTAA TATCGCTGCA
    TGTATGAGAA ATGAAAAGCT CGGGGAGGCT TGCTTCTACC TTATGGGCCA TAATCAAACT
    ACGACCCCGG CAGCGACGGG AACGGCCCCC CCACCGGTCC ACAAGGTGTT CCGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229247
    Clone ID Related Accession (Same CDS sequence) XM_024983165.1
    Accession Version XM_024983165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACTTCCA
    GATTATCAAG AACAGGACAT CTTCCTCTGG AGAAAAGAGA CCGGATTTGG ATTTAGGATT
    CTGGGTGGCA ATGAACCAGG GGAACCTATT TATATTGGTC ACATCGTACC ACTGGGTGCT
    GCTGATACAG ACGGCCGTCT GAGGTCTGGG GATGAATTAA TCTGTGTGGA TGGGACACCA
    GTCATTGGAA AATCACACCA GCTTGTGGTC CAGCTTATGC AACAAGCCGC CAAGCAAGGC
    CACGTCAATC TAACGGTGCG GCGTAAAGTG GTGTTTGCGG CCCCCAAAAC CGAGAACGAG
    GTACCCTCGC CAGCCTCATC CCATCACAGT AGCAACCAGC CGGCCTCGCT GACGGAGGAG
    AAGCGGACCC CGCAGGGCAG CCAGAACTCC CTGAACACGG TGAGCTCAGG CAGTGGCAGC
    ACCAGTGGCA TCGGCAGTGG CGGCGGTGGC GGCAGCGGTG TGGTGAGCAC CGTGGTCCAG
    CCCTATGACG TGGAGATCCG GCGCGGCGAG AACGAGGGCT TCGGCTTTGT CATCGTGTCA
    TCGGTGAGCA GGCCCGAGGC CGGCACGACG TTCGGTCGGA TTATTGAGGG GAGCCCTGCT
    GACCGATGCG GCAAGCTGAA AGTGGGAGAC CGGATCTTGG CAGTAAATGG ATGTTCCATC
    ACCAACAAAT CCCACTCTGA CATTGTCAAC CTCATCAAGG AAGCGGGCAA CACAGTCACC
    CTCCGCATCA TCCCTGGGGA TGAGTCTTCA AATGCCACTC TGCTGACCAA TGCGGAGAAG
    ATAGCCACCA TCACCACCAC ACACACCCCT TCGCAGCAAG GAGCCCAGGA GACCAGGAAT
    ACCACCAAAC CAAAGCCAGA ATCTCAGTTT GAATTCAAAG CACCCCAGGC AACACAGGAG
    CAAGATTTTT ACACTGTGGA GCTGGAAAGA GGAGCCAAGG GATTTGGTTT CAGTCTTCGA
    GGAGGTCGAG AGTATAACAT GGACCTCTAC GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC
    GAAAGGTGTG GGAAGATGAA GGTTGGTGAT GAAATTTTAG AGATCAATGG TGAGACCACC
    AAAAACATGA AGCATTCTCG GGCTATAGAA CTGATTAAGA ATGGTGGCCG CAGAGTGCGT
    CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC CCAGAATATG ACCCCGGCAG CGACGGGAAC
    GGCCCCCCCA CCGGTCCACA AGGTGTTCCG GAAATGAGGG CTGGGCCCCC GGACCGCCGA
    CAGCATCCAT CATTGGAGTC CAGCTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
    GAAAAGCGGG CACACGTGAG AGATCCCAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
    GAACATCACA CGTGGAACGG AACTTCTAGA AAACCCGACA GTGGGGCTTG CCGACCCAAG
    GACCGGGCAC CCGAGGGACG AAGAGACGCC CAACCCGAGC GGATGGTGAC CAATGGCCCC
    AAAAGGAGGT CTCCGGAGAA GCGGAGGGAA GGCACGCGCA GTGCTGACAA CACTTTGGAG
    AGAAGGGAGA AGCAGGAGAA GAGGAAGGAG GTTTCCCCGG AGAGGAGGCG AGAGCGGTCC
    CCGACCCGCC GGAGGGATGG CTCTCCAGGC CGGCGGCGGC GGTCTCTGGA GAGACTCTTG
    GATCAGAGAA GATCGCCCGA GCGCAAGAGA GGAAGCTCGC CCGAAAGGAG AGCCAAATCC
    ACCGACCGGA GGCGGACCAA GTCCCCCGAG CGGAGGAGAG AGCGGTCCCT GGAGAAAAGG
    AACAAAGAGG ACAGAGGCAG CCACCGGGAG AGGGACGAGA CGAGTCTAAG ACAGGACACC
    GGCCGGAGTT CCAGACATCC CCCGGAGCAG CGGAGAAGAC CTTACAAAGA ATGCAGCACC
    GACCTCAGTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838933.1
    CDS618..4952
    Translation

    Target ORF information:

    RefSeq Version XM_024983165.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACTTCCA
    GATTATCAAG AACAGGACAT CTTCCTCTGG AGAAAAGAGA CCGGATTTGG ATTTAGGATT
    CTGGGTGGCA ATGAACCAGG GGAACCTATT TATATTGGTC ACATCGTACC ACTGGGTGCT
    GCTGATACAG ACGGCCGTCT GAGGTCTGGG GATGAATTAA TCTGTGTGGA TGGGACACCA
    GTCATTGGAA AATCACACCA GCTTGTGGTC CAGCTTATGC AACAAGCCGC CAAGCAAGGC
    CACGTCAATC TAACGGTGCG GCGTAAAGTG GTGTTTGCGG CCCCCAAAAC CGAGAACGAG
    GTACCCTCGC CAGCCTCATC CCATCACAGT AGCAACCAGC CGGCCTCGCT GACGGAGGAG
    AAGCGGACCC CGCAGGGCAG CCAGAACTCC CTGAACACGG TGAGCTCAGG CAGTGGCAGC
    ACCAGTGGCA TCGGCAGTGG CGGCGGTGGC GGCAGCGGTG TGGTGAGCAC CGTGGTCCAG
    CCCTATGACG TGGAGATCCG GCGCGGCGAG AACGAGGGCT TCGGCTTTGT CATCGTGTCA
    TCGGTGAGCA GGCCCGAGGC CGGCACGACG TTCGGTCGGA TTATTGAGGG GAGCCCTGCT
    GACCGATGCG GCAAGCTGAA AGTGGGAGAC CGGATCTTGG CAGTAAATGG ATGTTCCATC
    ACCAACAAAT CCCACTCTGA CATTGTCAAC CTCATCAAGG AAGCGGGCAA CACAGTCACC
    CTCCGCATCA TCCCTGGGGA TGAGTCTTCA AATGCCACTC TGCTGACCAA TGCGGAGAAG
    ATAGCCACCA TCACCACCAC ACACACCCCT TCGCAGCAAG GAGCCCAGGA GACCAGGAAT
    ACCACCAAAC CAAAGCCAGA ATCTCAGTTT GAATTCAAAG CACCCCAGGC AACACAGGAG
    CAAGATTTTT ACACTGTGGA GCTGGAAAGA GGAGCCAAGG GATTTGGTTT CAGTCTTCGA
    GGAGGTCGAG AGTATAACAT GGACCTCTAC GTTCTGCGCT TAGCAGAGGA TGGTCCTGCC
    GAAAGGTGTG GGAAGATGAA GGTTGGTGAT GAAATTTTAG AGATCAATGG TGAGACCACC
    AAAAACATGA AGCATTCTCG GGCTATAGAA CTGATTAAGA ATGGTGGCCG CAGAGTGCGT
    CTGTTTCTGA AGCGGGGAGA CGGCTCGGTC CCAGAATATG ACCCCGGCAG CGACGGGAAC
    GGCCCCCCCA CCGGTCCACA AGGTGTTCCG GAAATGAGGG CTGGGCCCCC GGACCGCCGA
    CAGCATCCAT CATTGGAGTC CAGCTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
    GAAAAGCGGG CACACGTGAG AGATCCCAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
    GAACATCACA CGTGGAACGG AACTTCTAGA AAACCCGACA GTGGGGCTTG CCGACCCAAG
    GACCGGGCAC CCGAGGGACG AAGAGACGCC CAACCCGAGC GGATGGTGAC CAATGGCCCC
    AAAAGGAGGT CTCCGGAGAA GCGGAGGGAA GGCACGCGCA GTGCTGACAA CACTTTGGAG
    AGAAGGGAGA AGCAGGAGAA GAGGAAGGAG GTTTCCCCGG AGAGGAGGCG AGAGCGGTCC
    CCGACCCGCC GGAGGGATGG CTCTCCAGGC CGGCGGCGGC GGTCTCTGGA GAGACTCTTG
    GATCAGAGAA GATCGCCCGA GCGCAAGAGA GGAAGCTCGC CCGAAAGGAG AGCCAAATCC
    ACCGACCGGA GGCGGACCAA GTCCCCCGAG CGGAGGAGAG AGCGGTCCCT GGAGAAAAGG
    AACAAAGAGG ACAGAGGCAG CCACCGGGAG AGGGACGAGA CGAGTCTAAG ACAGGACACC
    GGCCGGAGTT CCAGACATCC CCCGGAGCAG CGGAGAAGAC CTTACAAAGA ATGCAGCACC
    GACCTCAGTA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229248
    Clone ID Related Accession (Same CDS sequence) XM_024983167.1
    Accession Version XM_024983167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACTTCCAGAT
    TATCAAGAAC AGGACATCTT CCTCTGGAGA AAAGAGACCG GATTTGGATT TAGGATTCTG
    GGTGGCAATG AACCAGGGGA ACCTATTTAT ATTGGTCACA TCGTACCACT GGGTGCTGCT
    GATACAGACG GCCGTCTGAG GTCTGGGGAT GAATTAATCT GTGTGGATGG GACACCAGTC
    ATTGGAAAAT CACACCAGCT TGTGGTCCAG CTTATGCAAC AAGCCGCCAA GCAAGGCCAC
    GTCAATCTAA CGGTGCGGCG TAAAGTGGTG TTTGCGGCCC CCAAAACCGA GAACGAGGTA
    CCCTCGCCAG CCTCATCCCA TCACAGTAGC AACCAGCCGG CCTCGCTGAC GGAGGAGAAG
    CGGACCCCGC AGGGCAGCCA GAACTCCCTG AACACGGTGA GCTCAGGCAG TGGCAGCACC
    AGTGGCATCG GCAGTGGCGG CGGTGGCGGC AGCGGTGTGG TGAGCACCGT GGTCCAGCCC
    TATGACGTGG AGATCCGGCG CGGCGAGAAC GAGGGCTTCG GCTTTGTCAT CGTGTCATCG
    GTGAGCAGGC CCGAGGCCGG CACGACGTTC GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838935.1
    CDS618..4949
    Translation

    Target ORF information:

    RefSeq Version XM_024983167.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACTTCCAGAT
    TATCAAGAAC AGGACATCTT CCTCTGGAGA AAAGAGACCG GATTTGGATT TAGGATTCTG
    GGTGGCAATG AACCAGGGGA ACCTATTTAT ATTGGTCACA TCGTACCACT GGGTGCTGCT
    GATACAGACG GCCGTCTGAG GTCTGGGGAT GAATTAATCT GTGTGGATGG GACACCAGTC
    ATTGGAAAAT CACACCAGCT TGTGGTCCAG CTTATGCAAC AAGCCGCCAA GCAAGGCCAC
    GTCAATCTAA CGGTGCGGCG TAAAGTGGTG TTTGCGGCCC CCAAAACCGA GAACGAGGTA
    CCCTCGCCAG CCTCATCCCA TCACAGTAGC AACCAGCCGG CCTCGCTGAC GGAGGAGAAG
    CGGACCCCGC AGGGCAGCCA GAACTCCCTG AACACGGTGA GCTCAGGCAG TGGCAGCACC
    AGTGGCATCG GCAGTGGCGG CGGTGGCGGC AGCGGTGTGG TGAGCACCGT GGTCCAGCCC
    TATGACGTGG AGATCCGGCG CGGCGAGAAC GAGGGCTTCG GCTTTGTCAT CGTGTCATCG
    GTGAGCAGGC CCGAGGCCGG CACGACGTTC GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229249
    Clone ID Related Accession (Same CDS sequence) XM_024983168.1
    Accession Version XM_024983168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACTTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838936.1
    CDS618..4949
    Translation

    Target ORF information:

    RefSeq Version XM_024983168.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACTTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229250
    Clone ID Related Accession (Same CDS sequence) XM_024983175.1
    Accession Version XM_024983175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGAG
    TCTTCAAATG CCACTCTGCT GACCAATGCG GAGAAGATAG CCACCATCAC CACCACACAC
    ACCCCTTCGC AGCAAGGAGC CCAGGAGACC AGGAATACCA CCAAACCAAA GCCAGAATCT
    CAGTTTGAAT TCAAAGCACC CCAGGCAACA CAGGAGCAAG ATTTTTACAC TGTGGAGCTG
    GAAAGAGGAG CCAAGGGATT TGGTTTCAGT CTTCGAGGAG GTCGAGAGTA TAACATGGAC
    CTCTACGTTC TGCGCTTAGC AGAGGATGGT CCTGCCGAAA GGTGTGGGAA GATGAAGGTT
    GGTGATGAAA TTTTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAGCA TTCTCGGGCT
    ATAGAACTGA TTAAGAATGG TGGCCGCAGA GTGCGTCTGT TTCTGAAGCG GGGAGACGGC
    TCGGTCCCAG AATATGCGAT GATACCTCCT AATATCGCTG CATGTATGAG AAATGAAAAG
    CTCGGGGAGG CTTGCTTCTA CCTTATGGGC CATAATCAAA CTACGACCCC GGCAGCGACG
    GGAACGGCCC CCCCACCGGT CCACAAGGTG TTCCGGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838943.1
    CDS620..4261
    Translation

    Target ORF information:

    RefSeq Version XM_024983175.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGAG
    TCTTCAAATG CCACTCTGCT GACCAATGCG GAGAAGATAG CCACCATCAC CACCACACAC
    ACCCCTTCGC AGCAAGGAGC CCAGGAGACC AGGAATACCA CCAAACCAAA GCCAGAATCT
    CAGTTTGAAT TCAAAGCACC CCAGGCAACA CAGGAGCAAG ATTTTTACAC TGTGGAGCTG
    GAAAGAGGAG CCAAGGGATT TGGTTTCAGT CTTCGAGGAG GTCGAGAGTA TAACATGGAC
    CTCTACGTTC TGCGCTTAGC AGAGGATGGT CCTGCCGAAA GGTGTGGGAA GATGAAGGTT
    GGTGATGAAA TTTTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAGCA TTCTCGGGCT
    ATAGAACTGA TTAAGAATGG TGGCCGCAGA GTGCGTCTGT TTCTGAAGCG GGGAGACGGC
    TCGGTCCCAG AATATGCGAT GATACCTCCT AATATCGCTG CATGTATGAG AAATGAAAAG
    CTCGGGGAGG CTTGCTTCTA CCTTATGGGC CATAATCAAA CTACGACCCC GGCAGCGACG
    GGAACGGCCC CCCCACCGGT CCACAAGGTG TTCCGGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229251
    Clone ID Related Accession (Same CDS sequence) XM_024983176.1
    Accession Version XM_024983176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACTTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGAGTCT
    TCAAATGCCA CTCTGCTGAC CAATGCGGAG AAGATAGCCA CCATCACCAC CACACACACC
    CCTTCGCAGC AAGGAGCCCA GGAGACCAGG AATACCACCA AACCAAAGCC AGAATCTCAG
    TTTGAATTCA AAGCACCCCA GGCAACACAG GAGCAAGATT TTTACACTGT GGAGCTGGAA
    AGAGGAGCCA AGGGATTTGG TTTCAGTCTT CGAGGAGGTC GAGAGTATAA CATGGACCTC
    TACGTTCTGC GCTTAGCAGA GGATGGTCCT GCCGAAAGGT GTGGGAAGAT GAAGGTTGGT
    GATGAAATTT TAGAGATCAA TGGTGAGACC ACCAAAAACA TGAAGCATTC TCGGGCTATA
    GAACTGATTA AGAATGGTGG CCGCAGAGTG CGTCTGTTTC TGAAGCGGGG AGACGGCTCG
    GTCCCAGAAT ATGCGATGAT ACCTCCTAAT ATCGCTGCAT GTATGAGAAA TGAAAAGCTC
    GGGGAGGCTT GCTTCTACCT TATGGGCCAT AATCAAACTA CGACCCCGGC AGCGACGGGA
    ACGGCCCCCC CACCGGTCCA CAAGGTGTTC CGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838944.1
    CDS619..4137
    Translation

    Target ORF information:

    RefSeq Version XM_024983176.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACTTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGAGTCT
    TCAAATGCCA CTCTGCTGAC CAATGCGGAG AAGATAGCCA CCATCACCAC CACACACACC
    CCTTCGCAGC AAGGAGCCCA GGAGACCAGG AATACCACCA AACCAAAGCC AGAATCTCAG
    TTTGAATTCA AAGCACCCCA GGCAACACAG GAGCAAGATT TTTACACTGT GGAGCTGGAA
    AGAGGAGCCA AGGGATTTGG TTTCAGTCTT CGAGGAGGTC GAGAGTATAA CATGGACCTC
    TACGTTCTGC GCTTAGCAGA GGATGGTCCT GCCGAAAGGT GTGGGAAGAT GAAGGTTGGT
    GATGAAATTT TAGAGATCAA TGGTGAGACC ACCAAAAACA TGAAGCATTC TCGGGCTATA
    GAACTGATTA AGAATGGTGG CCGCAGAGTG CGTCTGTTTC TGAAGCGGGG AGACGGCTCG
    GTCCCAGAAT ATGCGATGAT ACCTCCTAAT ATCGCTGCAT GTATGAGAAA TGAAAAGCTC
    GGGGAGGCTT GCTTCTACCT TATGGGCCAT AATCAAACTA CGACCCCGGC AGCGACGGGA
    ACGGCCCCCC CACCGGTCCA CAAGGTGTTC CGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57400
    Clone ID Related Accession (Same CDS sequence) XM_024983157.1
    Accession Version XM_024983157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838925.1
    CDS618..5069
    Translation

    Target ORF information:

    RefSeq Version XM_024983157.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57401
    Clone ID Related Accession (Same CDS sequence) XM_024983158.1
    Accession Version XM_024983158.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838926.1
    CDS618..5069
    Translation

    Target ORF information:

    RefSeq Version XM_024983158.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983158.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGAAGGG ATCCATACGG AGGAGCTGGA CAGTGAACTA
    GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
    CACATCAACA GGAAGACACA ATATGAGAAC CCAGTTCTAG AAGCCAAACG GAAGAAGCAG
    CTGGAGCAGC AGCAACAACA ACAACAGCAA CAGCAACAGC AGCCAGAAGA ATGGACAGAA
    GATCATTCAT CGCTTGTGCC TCCTGCTATT CCAAACCACC CCCCAAGTAA TCCGGAGCCA
    GCCAGAGAAG CTCCACTTCA GGGCAAACCC TTTTTTACAC GAAACCCTTC AGAGTTGAAA
    GGCAAATTCA TTCACACAAA GCTGCGGAAA AGCAGTCGTG GCTTTGGCTT CACAGTTGTT
    GGAGGGGATG AACCTGACGA GTTTCTACAA ATCAAGAGCT TGGTCCTAGA TGGTCCTGCC
    GCCCTGGATG GCAAGATGGA AACAGGAGAC GTAATTGTGA GCGTGAATGA CACCTGTGTT
    TTGGGACACA CGCATGCACA AGTTGTGAAA ATCTTCCAGT CCATTCCCAT CGGTGCCAGC
    GTGGACCTTG AACTCTGCCG AGGTTATCCA CTACCCTTTG ACCCAGATGA CCCCAACACG
    AGTTTAGTGA CTTCGGTGGC CATTCTGGAT AAAGAACCAA TTATTGTGAA TGGGCAAGAG
    ACCTATGACT CGCCAGCTAG TCACAGTAGT AAAACAGGCA AAGTCAGCAG TATGAAGGAT
    GCCAGGCCAA GCAGCCCTGC GGATGTGGCT TCAAATGGTT CCCATGGTTA CCCCACTGAC
    ACTGTCTCTT TGGCTTCCTC CATAGCCACT CAGCCAGAAC TCATAACTGT TCATATAGTG
    AAAGGGCCAA TGGGTTTTGG TTTTACTATC GCGGACAGTC CTGGGGGTGG CGGCCAAAGA
    GTGAAACAGA TTGTTGACAG TCCAAGGTGC CGAGGCCTGA AAGAAGGGGA TCTTATAGTG
    GAAGTTAATA AGAAGAATGT GCAGGCCCTA ACTCACAACC AAGTCGTGGA CATGCTCATC
    GAATGTCCAA AGGGAAGTGA GGTCACATTA TTGGTGCAAC GAGGAGGACT GCCAGTTCCC
    AAGAAGAGCC CAAAGTCGCA ACCACTGGAA AGAAAAGACA GCCAGAATAG TTCCCAGCAT
    AGTGTCGCCA GCCACCGAAG CCTGCACACT GCCTCCCCCA GCCACAGCAC ACAGGTGCTG
    CCCGAGCTCC CACCCGCCGA GGCCCCGGCT CCAGATCAGA CCGACAATTC TGGGCAGAAA
    AAACCAGATC CTTTTAAAAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAA TCGACCTATG
    TCACCTTCGC CTGCATCGGG ATTGAGCAAG GGTGAAAGAG AGAGAGAAAT CAATTCCACG
    AATTTTGGAG AATGTCAGAT TCCAGATTAT CAAGAACAGG ACATCTTCCT CTGGAGAAAA
    GAGACCGGAT TTGGATTTAG GATTCTGGGT GGCAATGAAC CAGGGGAACC TATTTATATT
    GGTCACATCG TACCACTGGG TGCTGCTGAT ACAGACGGCC GTCTGAGGTC TGGGGATGAA
    TTAATCTGTG TGGATGGGAC ACCAGTCATT GGAAAATCAC ACCAGCTTGT GGTCCAGCTT
    ATGCAACAAG CCGCCAAGCA AGGCCACGTC AATCTAACGG TGCGGCGTAA AGTGGTGTTT
    GCGGCCCCCA AAACCGAGAA CGAGGTACCC TCGCCAGCCT CATCCCATCA CAGTAGCAAC
    CAGCCGGCCT CGCTGACGGA GGAGAAGCGG ACCCCGCAGG GCAGCCAGAA CTCCCTGAAC
    ACGGTGAGCT CAGGCAGTGG CAGCACCAGT GGCATCGGCA GTGGCGGCGG TGGCGGCAGC
    GGTGTGGTGA GCACCGTGGT CCAGCCCTAT GACGTGGAGA TCCGGCGCGG CGAGAACGAG
    GGCTTCGGCT TTGTCATCGT GTCATCGGTG AGCAGGCCCG AGGCCGGCAC GACGTTCGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGACC CCGGCAGCGA CGGGAACGGC
    CCCCCCACCG GTCCACAAGG TGTTCCGGAA ATGAGGGCTG GGCCCCCGGA CCGCCGACAG
    CATCCATCAT TGGAGTCCAG CTACCCACCC GATCTTCACA AATCATCACC ACATGGGGAA
    AAGCGGGCAC ACGTGAGAGA TCCCAAAGGC AGCAGAGAGT ATAGCAGACA ACCCAATGAA
    CATCACACGT GGAACGGAAC TTCTAGAAAA CCCGACAGTG GGGCTTGCCG ACCCAAGGAC
    CGGGCACCCG AGGGACGAAG AGACGCCCAA CCCGAGCGGA TGGTGACCAA TGGCCCCAAA
    AGGAGGTCTC CGGAGAAGCG GAGGGAAGGC ACGCGCAGTG CTGACAACAC TTTGGAGAGA
    AGGGAGAAGC AGGAGAAGAG GAAGGAGGTT TCCCCGGAGA GGAGGCGAGA GCGGTCCCCG
    ACCCGCCGGA GGGATGGCTC TCCAGGCCGG CGGCGGCGGT CTCTGGAGAG ACTCTTGGAT
    CAGAGAAGAT CGCCCGAGCG CAAGAGAGGA AGCTCGCCCG AAAGGAGAGC CAAATCCACC
    GACCGGAGGC GGACCAAGTC CCCCGAGCGG AGGAGAGAGC GGTCCCTGGA GAAAAGGAAC
    AAAGAGGACA GAGGCAGCCA CCGGGAGAGG GACGAGACGA GTCTAAGACA GGACACCGGC
    CGGAGTTCCA GACATCCCCC GGAGCAGCGG AGAAGACCTT ACAAAGAATG CAGCACCGAC
    CTCAGTATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57402
    Clone ID Related Accession (Same CDS sequence) XM_024983174.1
    Accession Version XM_024983174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGGTG GGTCAAACTA TGAAAACATT
    CCTTCCTTCC CTGGCATGAC TCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838942.1
    CDS619..4365
    Translation

    Target ORF information:

    RefSeq Version XM_024983174.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAACA ACAGCAACAG CAACAGCAGC CAGAAGAATG GACAGAAGAT
    CATTCATCGC TTGTGCCTCC TGCTATTCCA AACCACCCCC CAAGTAATCC GGAGCCAGCC
    AGAGAAGCTC CACTTCAGGG CAAACCCTTT TTTACACGAA ACCCTTCAGA GTTGAAAGGC
    AAATTCATTC ACACAAAGCT GCGGAAAAGC AGTCGTGGCT TTGGCTTCAC AGTTGTTGGA
    GGGGATGAAC CTGACGAGTT TCTACAAATC AAGAGCTTGG TCCTAGATGG TCCTGCCGCC
    CTGGATGGCA AGATGGAAAC AGGAGACGTA ATTGTGAGCG TGAATGACAC CTGTGTTTTG
    GGACACACGC ATGCACAAGT TGTGAAAATC TTCCAGTCCA TTCCCATCGG TGCCAGCGTG
    GACCTTGAAC TCTGCCGAGG TTATCCACTA CCCTTTGACC CAGATGACCC CAACACGAGT
    TTAGTGACTT CGGTGGCCAT TCTGGATAAA GAACCAATTA TTGTGAATGG GCAAGAGACC
    TATGACTCGC CAGCTAGTCA CAGTAGTAAA ACAGGCAAAG TCAGCAGTAT GAAGGATGCC
    AGGCCAAGCA GCCCTGCGGA TGTGGCTTCA AATGGTTCCC ATGGTTACCC CACTGACACT
    GTCTCTTTGG CTTCCTCCAT AGCCACTCAG CCAGAACTCA TAACTGTTCA TATAGTGAAA
    GGGCCAATGG GTTTTGGTTT TACTATCGCG GACAGTCCTG GGGGTGGCGG CCAAAGAGTG
    AAACAGATTG TTGACAGTCC AAGGTGCCGA GGCCTGAAAG AAGGGGATCT TATAGTGGAA
    GTTAATAAGA AGAATGTGCA GGCCCTAACT CACAACCAAG TCGTGGACAT GCTCATCGAA
    TGTCCAAAGG GAAGTGAGGT CACATTATTG GTGCAACGAG GAGGACTGCC AGTTCCCAAG
    AAGAGCCCAA AGTCGCAACC ACTGGAAAGA AAAGACAGCC AGAATAGTTC CCAGCATAGT
    GTCGCCAGCC ACCGAAGCCT GCACACTGCC TCCCCCAGCC ACAGCACACA GGTGCTGCCC
    GAGCTCCCAC CCGCCGAGGC CCCGGCTCCA GATCAGACCG ACAATTCTGG GCAGAAAAAA
    CCAGATCCTT TTAAAATCTG GGCCCAGTCC AGGAGCATGT ATGAAAATCG ACCTATGTCA
    CCTTCGCCTG CATCGGGATT GAGCAAGGGT GAAAGAGAGA GAGAAATCAA TTCCACGAAT
    TTTGGAGAAT GTCAGATTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGCAGGC
    AATGCATGTG TGGCCATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
    CGATGCGGCA AGCTGAAAGT GGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
    AACAAATCCC ACTCTGACAT TGTCAACCTC ATCAAGGAAG CGGGCAACAC AGTCACCCTC
    CGCATCATCC CTGGGGATGA GTCTTCAAAT GCCACTCTGC TGACCAATGC GGAGAAGATA
    GCCACCATCA CCACCACACA CACCCCTTCG CAGCAAGGAG CCCAGGAGAC CAGGAATACC
    ACCAAACCAA AGCCAGAATC TCAGTTTGAA TTCAAAGCAC CCCAGGCAAC ACAGGAGCAA
    GATTTTTACA CTGTGGAGCT GGAAAGAGGA GCCAAGGGAT TTGGTTTCAG TCTTCGAGGA
    GGTCGAGAGT ATAACATGGA CCTCTACGTT CTGCGCTTAG CAGAGGATGG TCCTGCCGAA
    AGGTGTGGGA AGATGAAGGT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
    AACATGAAGC ATTCTCGGGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTGCGTCTG
    TTTCTGAAGC GGGGAGACGG CTCGGTCCCA GAATATGGTG GGTCAAACTA TGAAAACATT
    CCTTCCTTCC CTGGCATGAC TCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57403
    Clone ID Related Accession (Same CDS sequence) XM_024983169.1
    Accession Version XM_024983169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACTTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGGCAAT
    GCATGTGTGG CCATGCCTCA CAAAATAGGT CGGATTATTG AGGGGAGCCC TGCTGACCGA
    TGCGGCAAGC TGAAAGTGGG AGACCGGATC TTGGCAGTAA ATGGATGTTC CATCACCAAC
    AAATCCCACT CTGACATTGT CAACCTCATC AAGGAAGCGG GCAACACAGT CACCCTCCGC
    ATCATCCCTG GGGATGAGTC TTCAAATGCC ACTCTGCTGA CCAATGCGGA GAAGATAGCC
    ACCATCACCA CCACACACAC CCCTTCGCAG CAAGGAGCCC AGGAGACCAG GAATACCACC
    AAACCAAAGC CAGAATCTCA GTTTGAATTC AAAGCACCCC AGGCAACACA GGAGCAAGAT
    TTTTACACTG TGGAGCTGGA AAGAGGAGCC AAGGGATTTG GTTTCAGTCT TCGAGGAGGT
    CGAGAGTATA ACATGGACCT CTACGTTCTG CGCTTAGCAG AGGATGGTCC TGCCGAAAGG
    TGTGGGAAGA TGAAGGTTGG TGATGAAATT TTAGAGATCA ATGGTGAGAC CACCAAAAAC
    ATGAAGCATT CTCGGGCTAT AGAACTGATT AAGAATGGTG GCCGCAGAGT GCGTCTGTTT
    CTGAAGCGGG GAGACGGCTC GGTCCCAGAA TATGACCCCG GCAGCGACGG GAACGGCCCC
    CCCACCGGTC CACAAGGTGT TCCGGAAATG AGGGCTGGGC CCCCGGACCG CCGACAGCAT
    CCATCATTGG AGTCCAGCTA CCCACCCGAT CTTCACAAAT CATCACCACA TGGGGAAAAG
    CGGGCACACG TGAGAGATCC CAAAGGCAGC AGAGAGTATA GCAGACAACC CAATGAACAT
    CACACGTGGA ACGGAACTTC TAGAAAACCC GACAGTGGGG CTTGCCGACC CAAGGACCGG
    GCACCCGAGG GACGAAGAGA CGCCCAACCC GAGCGGATGG TGACCAATGG CCCCAAAAGG
    AGGTCTCCGG AGAAGCGGAG GGAAGGCACG CGCAGTGCTG ACAACACTTT GGAGAGAAGG
    GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC CCGGAGAGGA GGCGAGAGCG GTCCCCGACC
    CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG CGGCGGTCTC TGGAGAGACT CTTGGATCAG
    AGAAGATCGC CCGAGCGCAA GAGAGGAAGC TCGCCCGAAA GGAGAGCCAA ATCCACCGAC
    CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG AGAGAGCGGT CCCTGGAGAA AAGGAACAAA
    GAGGACAGAG GCAGCCACCG GGAGAGGGAC GAGACGAGTC TAAGACAGGA CACCGGCCGG
    AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA AGACCTTACA AAGAATGCAG CACCGACCTC
    AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838937.1
    CDS618..4946
    Translation

    Target ORF information:

    RefSeq Version XM_024983169.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATAAC
    TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
    TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCAGTTCTA
    GAAGCCAAAC GGAAGAAGCA GCTGGAGCAG CAGCAACAAC AACAACAGCA ACAGCAACAG
    CAGCCAGAAG AATGGACAGA AGATCATTCA TCGCTTGTGC CTCCTGCTAT TCCAAACCAC
    CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA GCTCCACTTC AGGGCAAACC CTTTTTTACA
    CGAAACCCTT CAGAGTTGAA AGGCAAATTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
    GGCTTTGGCT TCACAGTTGT TGGAGGGGAT GAACCTGACG AGTTTCTACA AATCAAGAGC
    TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT GGCAAGATGG AAACAGGAGA CGTAATTGTG
    AGCGTGAATG ACACCTGTGT TTTGGGACAC ACGCATGCAC AAGTTGTGAA AATCTTCCAG
    TCCATTCCCA TCGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ACTACCCTTT
    GACCCAGATG ACCCCAACAC GAGTTTAGTG ACTTCGGTGG CCATTCTGGA TAAAGAACCA
    ATTATTGTGA ATGGGCAAGA GACCTATGAC TCGCCAGCTA GTCACAGTAG TAAAACAGGC
    AAAGTCAGCA GTATGAAGGA TGCCAGGCCA AGCAGCCCTG CGGATGTGGC TTCAAATGGT
    TCCCATGGTT ACCCCACTGA CACTGTCTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
    CTCATAACTG TTCATATAGT GAAAGGGCCA ATGGGTTTTG GTTTTACTAT CGCGGACAGT
    CCTGGGGGTG GCGGCCAAAG AGTGAAACAG ATTGTTGACA GTCCAAGGTG CCGAGGCCTG
    AAAGAAGGGG ATCTTATAGT GGAAGTTAAT AAGAAGAATG TGCAGGCCCT AACTCACAAC
    CAAGTCGTGG ACATGCTCAT CGAATGTCCA AAGGGAAGTG AGGTCACATT ATTGGTGCAA
    CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGAAAAGAC
    AGCCAGAATA GTTCCCAGCA TAGTGTCGCC AGCCACCGAA GCCTGCACAC TGCCTCCCCC
    AGCCACAGCA CACAGGTGCT GCCCGAGCTC CCACCCGCCG AGGCCCCGGC TCCAGATCAG
    ACCGACAATT CTGGGCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
    ATGTATGAAA ATCGACTTCC AGATTATCAA GAACAGGACA TCTTCCTCTG GAGAAAAGAG
    ACCGGATTTG GATTTAGGAT TCTGGGTGGC AATGAACCAG GGGAACCTAT TTATATTGGT
    CACATCGTAC CACTGGGTGC TGCTGATACA GACGGCCGTC TGAGGTCTGG GGATGAATTA
    ATCTGTGTGG ATGGGACACC AGTCATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
    CAACAAGCCG CCAAGCAAGG CCACGTCAAT CTAACGGTGC GGCGTAAAGT GGTGTTTGCG
    GCCCCCAAAA CCGAGAACGA GGTACCCTCG CCAGCCTCAT CCCATCACAG TAGCAACCAG
    CCGGCCTCGC TGACGGAGGA GAAGCGGACC CCGCAGGGCA GCCAGAACTC CCTGAACACG
    GTGAGCTCAG GCAGTGGCAG CACCAGTGGC ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT
    GTGGTGAGCA CCGTGGTCCA GCCCTATGAC GTGGAGATCC GGCGCGGCGA GAACGAGGGC
    TTCGGCTTTG TCATCGTGTC ATCGGTGAGC AGGCCCGAGG CCGGCACGAC GTTCGGCAAT
    GCATGTGTGG CCATGCCTCA CAAAATAGGT CGGATTATTG AGGGGAGCCC TGCTGACCGA
    TGCGGCAAGC TGAAAGTGGG AGACCGGATC TTGGCAGTAA ATGGATGTTC CATCACCAAC
    AAATCCCACT CTGACATTGT CAACCTCATC AAGGAAGCGG GCAACACAGT CACCCTCCGC
    ATCATCCCTG GGGATGAGTC TTCAAATGCC ACTCTGCTGA CCAATGCGGA GAAGATAGCC
    ACCATCACCA CCACACACAC CCCTTCGCAG CAAGGAGCCC AGGAGACCAG GAATACCACC
    AAACCAAAGC CAGAATCTCA GTTTGAATTC AAAGCACCCC AGGCAACACA GGAGCAAGAT
    TTTTACACTG TGGAGCTGGA AAGAGGAGCC AAGGGATTTG GTTTCAGTCT TCGAGGAGGT
    CGAGAGTATA ACATGGACCT CTACGTTCTG CGCTTAGCAG AGGATGGTCC TGCCGAAAGG
    TGTGGGAAGA TGAAGGTTGG TGATGAAATT TTAGAGATCA ATGGTGAGAC CACCAAAAAC
    ATGAAGCATT CTCGGGCTAT AGAACTGATT AAGAATGGTG GCCGCAGAGT GCGTCTGTTT
    CTGAAGCGGG GAGACGGCTC GGTCCCAGAA TATGACCCCG GCAGCGACGG GAACGGCCCC
    CCCACCGGTC CACAAGGTGT TCCGGAAATG AGGGCTGGGC CCCCGGACCG CCGACAGCAT
    CCATCATTGG AGTCCAGCTA CCCACCCGAT CTTCACAAAT CATCACCACA TGGGGAAAAG
    CGGGCACACG TGAGAGATCC CAAAGGCAGC AGAGAGTATA GCAGACAACC CAATGAACAT
    CACACGTGGA ACGGAACTTC TAGAAAACCC GACAGTGGGG CTTGCCGACC CAAGGACCGG
    GCACCCGAGG GACGAAGAGA CGCCCAACCC GAGCGGATGG TGACCAATGG CCCCAAAAGG
    AGGTCTCCGG AGAAGCGGAG GGAAGGCACG CGCAGTGCTG ACAACACTTT GGAGAGAAGG
    GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC CCGGAGAGGA GGCGAGAGCG GTCCCCGACC
    CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG CGGCGGTCTC TGGAGAGACT CTTGGATCAG
    AGAAGATCGC CCGAGCGCAA GAGAGGAAGC TCGCCCGAAA GGAGAGCCAA ATCCACCGAC
    CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG AGAGAGCGGT CCCTGGAGAA AAGGAACAAA
    GAGGACAGAG GCAGCCACCG GGAGAGGGAC GAGACGAGTC TAAGACAGGA CACCGGCCGG
    AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA AGACCTTACA AAGAATGCAG CACCGACCTC
    AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31469
    Clone ID Related Accession (Same CDS sequence) XM_001789358.6 , XM_002696963.5
    Accession Version XM_001789358.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001789358.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATCTC
    TTTGGAAATG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAGCA ACAGCAACAG CAGCCAGAAG AATGGACAGA AGATCATTCA
    TCGCTTGTGC CTCCTGCTAT TCCAAACCAC CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA
    GCTCCACTTC AGGGCAAACC CTTTTTTACA CGAAACCCTT CAGAGTTGAA AGGCAAATTC
    ATTCACACAA AGCTGCGGAA AAGCAGTCGT GGCTTTGGCT TCACAGTTGT TGGAGGGGAT
    GAACCTGACG AGTTTCTACA AATCAAGAGC TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT
    GGCAAGATGG AAACAGGAGA CGTAATTGTG AGCGTGAATG ACACCTGTGT TTTGGGACAC
    ACGCATGCAC AAGTTGTGAA AATCTTCCAG TCCATTCCCA TCGGTGCCAG CGTGGACCTT
    GAACTCTGCC GAGGTTATCC ACTACCCTTT GACCCAGATG ACCCCAACAC GAGTTTAGTG
    ACTTCGGTGG CCATTCTGGA TAAAGAACCA ATTATTGTGA ATGGGCAAGA GACCTATGAC
    TCGCCAGCTA GTCACAGTAG TAAAACAGGC AAAGTCAGCA GTATGAAGGA TGCCAGGCCA
    AGCAGCCCTG CGGATGTGGC TTCAAATGGT TCCCATGGTT ACCCCACTGA CACTGTCTCT
    TTGGCTTCCT CCATAGCCAC TCAGCCAGAA CTCATAACTG TTCATATAGT GAAAGGGCCA
    ATGGGTTTTG GTTTTACTAT CGCGGACAGT CCTGGGGGTG GCGGCCAAAG AGTGAAACAG
    ATTGTTGACA GTCCAAGGTG CCGAGGCCTG AAAGAAGGGG ATCTTATAGT GGAAGTTAAT
    AAGAAGAATG TGCAGGCCCT AACTCACAAC CAAGTCGTGG ACATGCTCAT CGAATGTCCA
    AAGGGAAGTG AGGTCACATT ATTGGTGCAA CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC
    CCAAAGTCGC AACCACTGGA AAGAAAAGAC AGCCAGAATA GTTCCCAGCA TAGTGTCGCC
    AGCCACCGAA GCCTGCACAC TGCCTCCCCC AGCCACAGCA CACAGGTGCT GCCCGAGCTC
    CCACCCGCCG AGGCCCCGGC TCCAGATCAG ACCGACAATT CTGGGCAGAA AAAACCAGAT
    CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC ATGTATGAAA ATCGACTTCC AGATTATCAA
    GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACCGGATTTG GATTTAGGAT TCTGGGTGGC
    AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACA
    GACGGCCGTC TGAGGTCTGG GGATGAATTA ATCTGTGTGG ATGGGACACC AGTCATTGGA
    AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCCG CCAAGCAAGG CCACGTCAAT
    CTAACGGTGC GGCGTAAAGT GGTGTTTGCG GCCCCCAAAA CCGAGAACGA GGTACCCTCG
    CCAGCCTCAT CCCATCACAG TAGCAACCAG CCGGCCTCGC TGACGGAGGA GAAGCGGACC
    CCGCAGGGCA GCCAGAACTC CCTGAACACG GTGAGCTCAG GCAGTGGCAG CACCAGTGGC
    ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT GTGGTGAGCA CCGTGGTCCA GCCCTATGAC
    GTGGAGATCC GGCGCGGCGA GAACGAGGGC TTCGGCTTTG TCATCGTGTC ATCGGTGAGC
    AGGCCCGAGG CCGGCACGAC GTTCGGCAAT GCATGTGTGG CCATGCCTCA CAAAATAGGT
    CGGATTATTG AGGGGAGCCC TGCTGACCGA TGCGGCAAGC TGAAAGTGGG AGACCGGATC
    TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCACT CTGACATTGT CAACCTCATC
    AAGGAAGCGG GCAACACAGT CACCCTCCGC ATCATCCCTG GGGATGAGTC TTCAAATGCC
    ACTCTGCTGA CCAATGCGGA GAAGATAGCC ACCATCACCA CCACACACAC CCCTTCGCAG
    CAAGGAGCCC AGGAGACCAG GAATACCACC AAACCAAAGC CAGAATCTCA GTTTGAATTC
    AAAGCACCCC AGGCAACACA GGAGCAAGAT TTTTACACTG TGGAGCTGGA AAGAGGAGCC
    AAGGGATTTG GTTTCAGTCT TCGAGGAGGT CGAGAGTATA ACATGGACCT CTACGTTCTG
    CGCTTAGCAG AGGATGGTCC TGCCGAAAGG TGTGGGAAGA TGAAGGTTGG TGATGAAATT
    TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGGGCTAT AGAACTGATT
    AAGAATGGTG GCCGCAGAGT GCGTCTGTTT CTGAAGCGGG GAGACGGCTC GGTCCCAGAA
    TATGACCCCG GCAGCGACGG GAACGGCCCC CCCACCGGTC CACAAGGTGT TCCGGAAATG
    AGGGCTGGGC CCCCGGACCG CCGACAGCAT CCATCATTGG AGTCCAGCTA CCCACCCGAT
    CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG TGAGAGATCC CAAAGGCAGC
    AGAGAGTATA GCAGACAACC CAATGAACAT CACACGTGGA ACGGAACTTC TAGAAAACCC
    GACAGTGGGG CTTGCCGACC CAAGGACCGG GCACCCGAGG GACGAAGAGA CGCCCAACCC
    GAGCGGATGG TGACCAATGG CCCCAAAAGG AGGTCTCCGG AGAAGCGGAG GGAAGGCACG
    CGCAGTGCTG ACAACACTTT GGAGAGAAGG GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC
    CCGGAGAGGA GGCGAGAGCG GTCCCCGACC CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG
    CGGCGGTCTC TGGAGAGACT CTTGGATCAG AGAAGATCGC CCGAGCGCAA GAGAGGAAGC
    TCGCCCGAAA GGAGAGCCAA ATCCACCGAC CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG
    AGAGAGCGGT CCCTGGAGAA AAGGAACAAA GAGGACAGAG GCAGCCACCG GGAGAGGGAC
    GAGACGAGTC TAAGACAGGA CACCGGCCGG AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA
    AGACCTTACA AAGAATGCAG CACCGACCTC AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001789410.2
    CDS624..4982
    Translation

    Target ORF information:

    RefSeq Version XM_001789358.6
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001789358.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATCTC
    TTTGGAAATG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAGCA ACAGCAACAG CAGCCAGAAG AATGGACAGA AGATCATTCA
    TCGCTTGTGC CTCCTGCTAT TCCAAACCAC CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA
    GCTCCACTTC AGGGCAAACC CTTTTTTACA CGAAACCCTT CAGAGTTGAA AGGCAAATTC
    ATTCACACAA AGCTGCGGAA AAGCAGTCGT GGCTTTGGCT TCACAGTTGT TGGAGGGGAT
    GAACCTGACG AGTTTCTACA AATCAAGAGC TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT
    GGCAAGATGG AAACAGGAGA CGTAATTGTG AGCGTGAATG ACACCTGTGT TTTGGGACAC
    ACGCATGCAC AAGTTGTGAA AATCTTCCAG TCCATTCCCA TCGGTGCCAG CGTGGACCTT
    GAACTCTGCC GAGGTTATCC ACTACCCTTT GACCCAGATG ACCCCAACAC GAGTTTAGTG
    ACTTCGGTGG CCATTCTGGA TAAAGAACCA ATTATTGTGA ATGGGCAAGA GACCTATGAC
    TCGCCAGCTA GTCACAGTAG TAAAACAGGC AAAGTCAGCA GTATGAAGGA TGCCAGGCCA
    AGCAGCCCTG CGGATGTGGC TTCAAATGGT TCCCATGGTT ACCCCACTGA CACTGTCTCT
    TTGGCTTCCT CCATAGCCAC TCAGCCAGAA CTCATAACTG TTCATATAGT GAAAGGGCCA
    ATGGGTTTTG GTTTTACTAT CGCGGACAGT CCTGGGGGTG GCGGCCAAAG AGTGAAACAG
    ATTGTTGACA GTCCAAGGTG CCGAGGCCTG AAAGAAGGGG ATCTTATAGT GGAAGTTAAT
    AAGAAGAATG TGCAGGCCCT AACTCACAAC CAAGTCGTGG ACATGCTCAT CGAATGTCCA
    AAGGGAAGTG AGGTCACATT ATTGGTGCAA CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC
    CCAAAGTCGC AACCACTGGA AAGAAAAGAC AGCCAGAATA GTTCCCAGCA TAGTGTCGCC
    AGCCACCGAA GCCTGCACAC TGCCTCCCCC AGCCACAGCA CACAGGTGCT GCCCGAGCTC
    CCACCCGCCG AGGCCCCGGC TCCAGATCAG ACCGACAATT CTGGGCAGAA AAAACCAGAT
    CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC ATGTATGAAA ATCGACTTCC AGATTATCAA
    GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACCGGATTTG GATTTAGGAT TCTGGGTGGC
    AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACA
    GACGGCCGTC TGAGGTCTGG GGATGAATTA ATCTGTGTGG ATGGGACACC AGTCATTGGA
    AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCCG CCAAGCAAGG CCACGTCAAT
    CTAACGGTGC GGCGTAAAGT GGTGTTTGCG GCCCCCAAAA CCGAGAACGA GGTACCCTCG
    CCAGCCTCAT CCCATCACAG TAGCAACCAG CCGGCCTCGC TGACGGAGGA GAAGCGGACC
    CCGCAGGGCA GCCAGAACTC CCTGAACACG GTGAGCTCAG GCAGTGGCAG CACCAGTGGC
    ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT GTGGTGAGCA CCGTGGTCCA GCCCTATGAC
    GTGGAGATCC GGCGCGGCGA GAACGAGGGC TTCGGCTTTG TCATCGTGTC ATCGGTGAGC
    AGGCCCGAGG CCGGCACGAC GTTCGGCAAT GCATGTGTGG CCATGCCTCA CAAAATAGGT
    CGGATTATTG AGGGGAGCCC TGCTGACCGA TGCGGCAAGC TGAAAGTGGG AGACCGGATC
    TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCACT CTGACATTGT CAACCTCATC
    AAGGAAGCGG GCAACACAGT CACCCTCCGC ATCATCCCTG GGGATGAGTC TTCAAATGCC
    ACTCTGCTGA CCAATGCGGA GAAGATAGCC ACCATCACCA CCACACACAC CCCTTCGCAG
    CAAGGAGCCC AGGAGACCAG GAATACCACC AAACCAAAGC CAGAATCTCA GTTTGAATTC
    AAAGCACCCC AGGCAACACA GGAGCAAGAT TTTTACACTG TGGAGCTGGA AAGAGGAGCC
    AAGGGATTTG GTTTCAGTCT TCGAGGAGGT CGAGAGTATA ACATGGACCT CTACGTTCTG
    CGCTTAGCAG AGGATGGTCC TGCCGAAAGG TGTGGGAAGA TGAAGGTTGG TGATGAAATT
    TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGGGCTAT AGAACTGATT
    AAGAATGGTG GCCGCAGAGT GCGTCTGTTT CTGAAGCGGG GAGACGGCTC GGTCCCAGAA
    TATGACCCCG GCAGCGACGG GAACGGCCCC CCCACCGGTC CACAAGGTGT TCCGGAAATG
    AGGGCTGGGC CCCCGGACCG CCGACAGCAT CCATCATTGG AGTCCAGCTA CCCACCCGAT
    CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG TGAGAGATCC CAAAGGCAGC
    AGAGAGTATA GCAGACAACC CAATGAACAT CACACGTGGA ACGGAACTTC TAGAAAACCC
    GACAGTGGGG CTTGCCGACC CAAGGACCGG GCACCCGAGG GACGAAGAGA CGCCCAACCC
    GAGCGGATGG TGACCAATGG CCCCAAAAGG AGGTCTCCGG AGAAGCGGAG GGAAGGCACG
    CGCAGTGCTG ACAACACTTT GGAGAGAAGG GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC
    CCGGAGAGGA GGCGAGAGCG GTCCCCGACC CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG
    CGGCGGTCTC TGGAGAGACT CTTGGATCAG AGAAGATCGC CCGAGCGCAA GAGAGGAAGC
    TCGCCCGAAA GGAGAGCCAA ATCCACCGAC CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG
    AGAGAGCGGT CCCTGGAGAA AAGGAACAAA GAGGACAGAG GCAGCCACCG GGAGAGGGAC
    GAGACGAGTC TAAGACAGGA CACCGGCCGG AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA
    AGACCTTACA AAGAATGCAG CACCGACCTC AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31469
    Clone ID Related Accession (Same CDS sequence) XM_001789358.6 , XM_002696963.5
    Accession Version XM_002696963.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104541.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696963.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATCTC
    TTTGGAAATG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAGCA ACAGCAACAG CAGCCAGAAG AATGGACAGA AGATCATTCA
    TCGCTTGTGC CTCCTGCTAT TCCAAACCAC CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA
    GCTCCACTTC AGGGCAAACC CTTTTTTACA CGAAACCCTT CAGAGTTGAA AGGCAAATTC
    ATTCACACAA AGCTGCGGAA AAGCAGTCGT GGCTTTGGCT TCACAGTTGT TGGAGGGGAT
    GAACCTGACG AGTTTCTACA AATCAAGAGC TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT
    GGCAAGATGG AAACAGGAGA CGTAATTGTG AGCGTGAATG ACACCTGTGT TTTGGGACAC
    ACGCATGCAC AAGTTGTGAA AATCTTCCAG TCCATTCCCA TCGGTGCCAG CGTGGACCTT
    GAACTCTGCC GAGGTTATCC ACTACCCTTT GACCCAGATG ACCCCAACAC GAGTTTAGTG
    ACTTCGGTGG CCATTCTGGA TAAAGAACCA ATTATTGTGA ATGGGCAAGA GACCTATGAC
    TCGCCAGCTA GTCACAGTAG TAAAACAGGC AAAGTCAGCA GTATGAAGGA TGCCAGGCCA
    AGCAGCCCTG CGGATGTGGC TTCAAATGGT TCCCATGGTT ACCCCACTGA CACTGTCTCT
    TTGGCTTCCT CCATAGCCAC TCAGCCAGAA CTCATAACTG TTCATATAGT GAAAGGGCCA
    ATGGGTTTTG GTTTTACTAT CGCGGACAGT CCTGGGGGTG GCGGCCAAAG AGTGAAACAG
    ATTGTTGACA GTCCAAGGTG CCGAGGCCTG AAAGAAGGGG ATCTTATAGT GGAAGTTAAT
    AAGAAGAATG TGCAGGCCCT AACTCACAAC CAAGTCGTGG ACATGCTCAT CGAATGTCCA
    AAGGGAAGTG AGGTCACATT ATTGGTGCAA CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC
    CCAAAGTCGC AACCACTGGA AAGAAAAGAC AGCCAGAATA GTTCCCAGCA TAGTGTCGCC
    AGCCACCGAA GCCTGCACAC TGCCTCCCCC AGCCACAGCA CACAGGTGCT GCCCGAGCTC
    CCACCCGCCG AGGCCCCGGC TCCAGATCAG ACCGACAATT CTGGGCAGAA AAAACCAGAT
    CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC ATGTATGAAA ATCGACTTCC AGATTATCAA
    GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACCGGATTTG GATTTAGGAT TCTGGGTGGC
    AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACA
    GACGGCCGTC TGAGGTCTGG GGATGAATTA ATCTGTGTGG ATGGGACACC AGTCATTGGA
    AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCCG CCAAGCAAGG CCACGTCAAT
    CTAACGGTGC GGCGTAAAGT GGTGTTTGCG GCCCCCAAAA CCGAGAACGA GGTACCCTCG
    CCAGCCTCAT CCCATCACAG TAGCAACCAG CCGGCCTCGC TGACGGAGGA GAAGCGGACC
    CCGCAGGGCA GCCAGAACTC CCTGAACACG GTGAGCTCAG GCAGTGGCAG CACCAGTGGC
    ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT GTGGTGAGCA CCGTGGTCCA GCCCTATGAC
    GTGGAGATCC GGCGCGGCGA GAACGAGGGC TTCGGCTTTG TCATCGTGTC ATCGGTGAGC
    AGGCCCGAGG CCGGCACGAC GTTCGGCAAT GCATGTGTGG CCATGCCTCA CAAAATAGGT
    CGGATTATTG AGGGGAGCCC TGCTGACCGA TGCGGCAAGC TGAAAGTGGG AGACCGGATC
    TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCACT CTGACATTGT CAACCTCATC
    AAGGAAGCGG GCAACACAGT CACCCTCCGC ATCATCCCTG GGGATGAGTC TTCAAATGCC
    ACTCTGCTGA CCAATGCGGA GAAGATAGCC ACCATCACCA CCACACACAC CCCTTCGCAG
    CAAGGAGCCC AGGAGACCAG GAATACCACC AAACCAAAGC CAGAATCTCA GTTTGAATTC
    AAAGCACCCC AGGCAACACA GGAGCAAGAT TTTTACACTG TGGAGCTGGA AAGAGGAGCC
    AAGGGATTTG GTTTCAGTCT TCGAGGAGGT CGAGAGTATA ACATGGACCT CTACGTTCTG
    CGCTTAGCAG AGGATGGTCC TGCCGAAAGG TGTGGGAAGA TGAAGGTTGG TGATGAAATT
    TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGGGCTAT AGAACTGATT
    AAGAATGGTG GCCGCAGAGT GCGTCTGTTT CTGAAGCGGG GAGACGGCTC GGTCCCAGAA
    TATGACCCCG GCAGCGACGG GAACGGCCCC CCCACCGGTC CACAAGGTGT TCCGGAAATG
    AGGGCTGGGC CCCCGGACCG CCGACAGCAT CCATCATTGG AGTCCAGCTA CCCACCCGAT
    CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG TGAGAGATCC CAAAGGCAGC
    AGAGAGTATA GCAGACAACC CAATGAACAT CACACGTGGA ACGGAACTTC TAGAAAACCC
    GACAGTGGGG CTTGCCGACC CAAGGACCGG GCACCCGAGG GACGAAGAGA CGCCCAACCC
    GAGCGGATGG TGACCAATGG CCCCAAAAGG AGGTCTCCGG AGAAGCGGAG GGAAGGCACG
    CGCAGTGCTG ACAACACTTT GGAGAGAAGG GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC
    CCGGAGAGGA GGCGAGAGCG GTCCCCGACC CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG
    CGGCGGTCTC TGGAGAGACT CTTGGATCAG AGAAGATCGC CCGAGCGCAA GAGAGGAAGC
    TCGCCCGAAA GGAGAGCCAA ATCCACCGAC CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG
    AGAGAGCGGT CCCTGGAGAA AAGGAACAAA GAGGACAGAG GCAGCCACCG GGAGAGGGAC
    GAGACGAGTC TAAGACAGGA CACCGGCCGG AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA
    AGACCTTACA AAGAATGCAG CACCGACCTC AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697009.1
    CDS624..4982
    Translation

    Target ORF information:

    RefSeq Version XM_002696963.5
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696963.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGAAGG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTA 
    AAGCGGGGAC CCCAGGGAGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GAACGGAGAG
    TTTCCGTATG TTGGAGCAGT GACGGCTACC GAAGCGGCGG GGCTTCCCGG TGGCGGCGAG
    GGCCCTCGGC TGGGCGAGGG CGAGTTGCTT CTGGAGGTGC AGGGGATCCG GGTGTCCGGC
    TTGCCCCGCT ATGACGTACT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA
    GCCGTCAGAC AAGGAGGAAA GCTGAACAAA GACCTGCGAC ATTTTCTCAA TCAGAGGTTC
    CAGAAAGGGT CGCCTGATCA TGAGCTCCAG CAGACCATAA GAGATAACCT TTACCGCCAT
    GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAGG TGCCTGGCGT GGACTATCTC
    TTTGGAAATG TGAAGGAGTT CTTGGACCTC GAGCAGAGCG GGACTCTTCT AGAAGTTGGC
    ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCTA GCCAGCCAGT CAGTGGGAAA
    GTGATCACGA CGGATGCCTT GCAAAACCTT CACTCTGGCT CCAAGCAGTC GACCCCAAAG
    CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAA
    GAGGATGATG TTCCTGAAAT GAACAGTAGC TTTACAGCCG ATTCTGGTGA CCAAGAAGAG
    CACAGTCTCC AAGAAGCAGC ACACCCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
    ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
    CCTCTACCTG AAAACTGGGA GATGGCCTAT ACCGAAAATG GAGAAGTCTA TTTTATAGAC
    CACAACACAA AAACAACATC TTGGTTAGAC CCTCGGTGCC TGAACAAGCA GCAGAAGCCT
    CTGGAGGAGT GTGAAGATGA TGAAGGGATC CATACGGAGG AGCTGGACAG TGAACTAGAA
    CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
    ATCAACAGGA AGACACAATA TGAGAACCCA GTTCTAGAAG CCAAACGGAA GAAGCAGCTG
    GAGCAGCAGC AACAACAGCA ACAGCAACAG CAGCCAGAAG AATGGACAGA AGATCATTCA
    TCGCTTGTGC CTCCTGCTAT TCCAAACCAC CCCCCAAGTA ATCCGGAGCC AGCCAGAGAA
    GCTCCACTTC AGGGCAAACC CTTTTTTACA CGAAACCCTT CAGAGTTGAA AGGCAAATTC
    ATTCACACAA AGCTGCGGAA AAGCAGTCGT GGCTTTGGCT TCACAGTTGT TGGAGGGGAT
    GAACCTGACG AGTTTCTACA AATCAAGAGC TTGGTCCTAG ATGGTCCTGC CGCCCTGGAT
    GGCAAGATGG AAACAGGAGA CGTAATTGTG AGCGTGAATG ACACCTGTGT TTTGGGACAC
    ACGCATGCAC AAGTTGTGAA AATCTTCCAG TCCATTCCCA TCGGTGCCAG CGTGGACCTT
    GAACTCTGCC GAGGTTATCC ACTACCCTTT GACCCAGATG ACCCCAACAC GAGTTTAGTG
    ACTTCGGTGG CCATTCTGGA TAAAGAACCA ATTATTGTGA ATGGGCAAGA GACCTATGAC
    TCGCCAGCTA GTCACAGTAG TAAAACAGGC AAAGTCAGCA GTATGAAGGA TGCCAGGCCA
    AGCAGCCCTG CGGATGTGGC TTCAAATGGT TCCCATGGTT ACCCCACTGA CACTGTCTCT
    TTGGCTTCCT CCATAGCCAC TCAGCCAGAA CTCATAACTG TTCATATAGT GAAAGGGCCA
    ATGGGTTTTG GTTTTACTAT CGCGGACAGT CCTGGGGGTG GCGGCCAAAG AGTGAAACAG
    ATTGTTGACA GTCCAAGGTG CCGAGGCCTG AAAGAAGGGG ATCTTATAGT GGAAGTTAAT
    AAGAAGAATG TGCAGGCCCT AACTCACAAC CAAGTCGTGG ACATGCTCAT CGAATGTCCA
    AAGGGAAGTG AGGTCACATT ATTGGTGCAA CGAGGAGGAC TGCCAGTTCC CAAGAAGAGC
    CCAAAGTCGC AACCACTGGA AAGAAAAGAC AGCCAGAATA GTTCCCAGCA TAGTGTCGCC
    AGCCACCGAA GCCTGCACAC TGCCTCCCCC AGCCACAGCA CACAGGTGCT GCCCGAGCTC
    CCACCCGCCG AGGCCCCGGC TCCAGATCAG ACCGACAATT CTGGGCAGAA AAAACCAGAT
    CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC ATGTATGAAA ATCGACTTCC AGATTATCAA
    GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACCGGATTTG GATTTAGGAT TCTGGGTGGC
    AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACA
    GACGGCCGTC TGAGGTCTGG GGATGAATTA ATCTGTGTGG ATGGGACACC AGTCATTGGA
    AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCCG CCAAGCAAGG CCACGTCAAT
    CTAACGGTGC GGCGTAAAGT GGTGTTTGCG GCCCCCAAAA CCGAGAACGA GGTACCCTCG
    CCAGCCTCAT CCCATCACAG TAGCAACCAG CCGGCCTCGC TGACGGAGGA GAAGCGGACC
    CCGCAGGGCA GCCAGAACTC CCTGAACACG GTGAGCTCAG GCAGTGGCAG CACCAGTGGC
    ATCGGCAGTG GCGGCGGTGG CGGCAGCGGT GTGGTGAGCA CCGTGGTCCA GCCCTATGAC
    GTGGAGATCC GGCGCGGCGA GAACGAGGGC TTCGGCTTTG TCATCGTGTC ATCGGTGAGC
    AGGCCCGAGG CCGGCACGAC GTTCGGCAAT GCATGTGTGG CCATGCCTCA CAAAATAGGT
    CGGATTATTG AGGGGAGCCC TGCTGACCGA TGCGGCAAGC TGAAAGTGGG AGACCGGATC
    TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCACT CTGACATTGT CAACCTCATC
    AAGGAAGCGG GCAACACAGT CACCCTCCGC ATCATCCCTG GGGATGAGTC TTCAAATGCC
    ACTCTGCTGA CCAATGCGGA GAAGATAGCC ACCATCACCA CCACACACAC CCCTTCGCAG
    CAAGGAGCCC AGGAGACCAG GAATACCACC AAACCAAAGC CAGAATCTCA GTTTGAATTC
    AAAGCACCCC AGGCAACACA GGAGCAAGAT TTTTACACTG TGGAGCTGGA AAGAGGAGCC
    AAGGGATTTG GTTTCAGTCT TCGAGGAGGT CGAGAGTATA ACATGGACCT CTACGTTCTG
    CGCTTAGCAG AGGATGGTCC TGCCGAAAGG TGTGGGAAGA TGAAGGTTGG TGATGAAATT
    TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGGGCTAT AGAACTGATT
    AAGAATGGTG GCCGCAGAGT GCGTCTGTTT CTGAAGCGGG GAGACGGCTC GGTCCCAGAA
    TATGACCCCG GCAGCGACGG GAACGGCCCC CCCACCGGTC CACAAGGTGT TCCGGAAATG
    AGGGCTGGGC CCCCGGACCG CCGACAGCAT CCATCATTGG AGTCCAGCTA CCCACCCGAT
    CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG TGAGAGATCC CAAAGGCAGC
    AGAGAGTATA GCAGACAACC CAATGAACAT CACACGTGGA ACGGAACTTC TAGAAAACCC
    GACAGTGGGG CTTGCCGACC CAAGGACCGG GCACCCGAGG GACGAAGAGA CGCCCAACCC
    GAGCGGATGG TGACCAATGG CCCCAAAAGG AGGTCTCCGG AGAAGCGGAG GGAAGGCACG
    CGCAGTGCTG ACAACACTTT GGAGAGAAGG GAGAAGCAGG AGAAGAGGAA GGAGGTTTCC
    CCGGAGAGGA GGCGAGAGCG GTCCCCGACC CGCCGGAGGG ATGGCTCTCC AGGCCGGCGG
    CGGCGGTCTC TGGAGAGACT CTTGGATCAG AGAAGATCGC CCGAGCGCAA GAGAGGAAGC
    TCGCCCGAAA GGAGAGCCAA ATCCACCGAC CGGAGGCGGA CCAAGTCCCC CGAGCGGAGG
    AGAGAGCGGT CCCTGGAGAA AAGGAACAAA GAGGACAGAG GCAGCCACCG GGAGAGGGAC
    GAGACGAGTC TAAGACAGGA CACCGGCCGG AGTTCCAGAC ATCCCCCGGA GCAGCGGAGA
    AGACCTTACA AAGAATGCAG CACCGACCTC AGTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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