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EIF2B5 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol EIF2B5
Entrez Gene ID 783760
Full Name eukaryotic translation initiation factor 2B subunit epsilon
General protein information
Preferred Names

eukaryotic translation initiation factor 2B subunit epsilon

Names

translation initiation factor eIF-2B subunit epsilon
eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_001251995.5 XP_001251996.1 translation initiation factor eIF-2B subunit epsilon
XM_002684878.3 XP_002684924.1 translation initiation factor eIF-2B subunit epsilon
XM_002684878.4 XP_002684924.2 translation initiation factor eIF-2B subunit epsilon

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) EIF2B5 XP_001139517.1
Gallus gallus (chicken) EIF2B5 XP_422755.3
Drosophila melanogaster (fruit fly) eIF2B-epsilon NP_996329.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000082 XP_001687758.1
Arabidopsis thaliana (thale cress) AT2G34970 NP_181042.1
EIF2B5 XP_001104122.1
Danio rerio (zebrafish) eif2b5 NP_001107069.1
Schizosaccharomyces pombe (fission yeast) tif225 NP_594285.1
Arabidopsis thaliana (thale cress) AT4G18300 NP_193564.1
Xenopus tropicalis (tropical clawed frog) eif2b5 XP_002935185.2
Mus musculus (house mouse) Eif2b5 NP_758469.1
Saccharomyces cerevisiae (baker's yeast) GCD6 NP_010497.3
Eremothecium gossypii AGOS_AGL094W NP_986572.1
Canis lupus familiaris (dog) EIF2B5 XP_003640192.1
Bos taurus (cattle) EIF2B5 XP_002684924.1
Rattus norvegicus (Norway rat) Eif2b5 NP_620221.2
Kluyveromyces lactis KLLA0A04235g XP_451187.1
Neurospora crassa NCU02414 XP_959596.1
Homo sapiens (human) EIF2B5 NP_003898.2
Magnaporthe oryzae (rice blast fungus) MGG_03517 XP_003716402.1
Arabidopsis thaliana (thale cress) AT3G02270 NP_186876.1
Oryza sativa (rice) Os02g0812400 NP_001048478.1

Gene Ontology

The following EIF2B5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219185 XM_002684878.4
Latest version!		 Bos taurus eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OBa02578 XM_001251995.5
Latest version!		 Bos taurus eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $426.30
$609.00
OBa02578 XM_002684878.3
Latest version!		 Bos taurus eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $426.30
$609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa219185
Clone ID Related Accession (Same CDS sequence) XM_002684878.4
Accession Version XM_002684878.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product translation initiation factor eIF-2B subunit epsilon
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002684878.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCA CTGTGGTGGC ACCGCCTGGT GCGTTGGCTA GTCGGGTCAA CAAGCGCAGC 
GGCGGCGGAC CGGGAGGCGG TGGCGGCGGT GGTGGCGGCG GCGGTGGTGG GGCAGCCAGA 
GGGGCGGAGG AGGAACCGCC GCCGCCCCTA CAAGCAGTTC TGGTGGCCGA CAGCTTCAAC 
CGCCGCTTCT TTCCCATCTC CAAGGACCAG CCTCGGGTCC TCTTGCCCCT GGCCAATGTG 
GCACTAATTG ACTACACTCT GGAATTTCTG ACTGCCACAG GTGTACAGGA AACCTTTGTC 
TTTTGTTGCT GGAAGGCTGC TCAAATCAAG GAACATTTAC TGAAATCCAA GTGGTGCCGC 
CCAACATCCC TCAATGTGGT TCGAATAATT ACATCAGAGC TGTATAGATC ACTGGGGGAT 
GTTCTCCGTG ATGTTGATGC TAAAGCCTTG GTGCGCTCTG ACTTCCTTCT GGTGTATGGG 
GATGTCATCT CAAACATCAA TATTACCAGA GCCCTGGAAG AACACAGGTT AAGGCGGAAG 
CTAGAAAAAA ATGTATCTGT GATGACGATG ATCTTCAAGG AGTCATCCCC CAGCCACCCA 
ACTCGTTGTC ATGAGGATAA CGTGGTAGTG GCCGTGGATA GTGCCACAAA TCAGGTGCTC 
CATTTCCAGA AGACCCAAGG CCTCCGACGT TTTTCTTTCC CTCTGAGCTT GTTCCAGGGC 
AGTGGAGATG GAGTGGAGAT TCGCTATGAC TTACTTGATT GTCATATCAG CATCTGCTCT 
CCTCAGGTGG CTCAACTATT TACAGACAAC TTCGACTACC AAACACGAGA TGACTTTGTG 
CGAGGCCTGT TAGTAAATGA GGAGATCTTA GGAAACCAGA TCCACATGCA TGTGACAACT 
AAGGAATATG GTGCCCGGGT CTCCAACCTA CACATGTATG CTGCTGTCTG CGCGGATGTC 
ATCCGCCGAT GGGTCTACCC TCTCACCCCA GAGGCTAACT TCACTGACAG CACAACCCAG 
AGCTGCACTC ACTCCCGACA TAACATCTAC CGAGGGCCTG AGGTCAGCCT GGGCCATGGC 
AGCATCCTGG AGGAAAATGT GCTCTTAGGT TCTGGCACTG TCATTGGCAG CAATTGCTCC 
ATCACTAACA GTGTCATTGG CCCTGGCTGT CACATTGGTG ATAATGTGGT GCTGGACCAG 
GCCTTCCTAT GGCAGGGTGT TCGAGTGGCT GCTGGAGCAC AGATTCATCA GTCTCTGCTC 
TGTGACAATG CTGAGGTCAA GGAAAATGTT ACACTGAAGC CTCACTGTGT CCTCACTTCC 
CAGGTGGTAG TGGGCCCAGA TATCATGCTA CCTGAGGGCT CAGTGATCTC TTTGCACCCT 
CCAGATGCAG AGGATGATGA GGATGATGGT CAGTTCAGTG ATGATTCTGG GGCTGACCAA 
GAAAAAGAGA AAATGAAGCT GAAAGGTTAC AATCCAGCAG AAGTTGGAGT TGCAGGCCAG 
GGCTACCTCT GGAAAGCTGC AGATATGAAC ATGGAGGAAG AGGAGGAACT ACGGCAGAAT 
CTATGGGGAC TCAAAATCAA CATGGAAGAA GAGAGTGAAA CTGAAAGTGA GCGAAGTATG 
GATTCTGAAG AGCTAGACAG TCGGGCAGGC TCTCCACAGA TGGATGACAT CAAAGTGTTT 
CAGAATGAAG TCCTGGGAAC CCTGCAGCGG GGCAAGGAGG AAAACATTTC TTGTGACAAT 
CTAGTCCTGG AGATAAATTC TCTCAAGTAT GCCTACAACA TAAGCCTGAA GGAAGTGATG 
CAAGTACTGA GCCATGTGGT CCTGGAGTTC CCACTGCAAC AAATGGATTC CCCGCTAGAC 
CCAAACCGCT ATTGCACCCT GTTGATTCCT TTGCTCAAGG CCTGGAGCCC TGTCTTTAGG 
AACTACATAA AGCGCGCCGC TGATCATTTG GAATCTTTGG CAGCCATTGA GGACTTCTTT 
TTGGAGCATG AAGCTCTTAG CACTTCCATG GCCAAGGTAC TGATGGCTTT TTACCAACTG 
GAGATCCTGG CTGAGGAAAC AATTCTGAGC TGGTTCAGCC GAAGGGAGGT AACTGACAAA 
GGCCAGCAGT TGCGAAAGAA CCAGCAGCTG CAGAGGTTCA TCCAGTGGCT AAAAGAGGCA 
GAAGAGGAGT CATCTGAAGA TGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002684924.2
CDS35..2221
Translation

Target ORF information:

RefSeq Version XM_002684878.4
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002684878.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCA CTGTGGTGGC ACCGCCTGGT GCGTTGGCTA GTCGGGTCAA CAAGCGCAGC 
GGCGGCGGAC CGGGAGGCGG TGGCGGCGGT GGTGGCGGCG GCGGTGGTGG GGCAGCCAGA 
GGGGCGGAGG AGGAACCGCC GCCGCCCCTA CAAGCAGTTC TGGTGGCCGA CAGCTTCAAC 
CGCCGCTTCT TTCCCATCTC CAAGGACCAG CCTCGGGTCC TCTTGCCCCT GGCCAATGTG 
GCACTAATTG ACTACACTCT GGAATTTCTG ACTGCCACAG GTGTACAGGA AACCTTTGTC 
TTTTGTTGCT GGAAGGCTGC TCAAATCAAG GAACATTTAC TGAAATCCAA GTGGTGCCGC 
CCAACATCCC TCAATGTGGT TCGAATAATT ACATCAGAGC TGTATAGATC ACTGGGGGAT 
GTTCTCCGTG ATGTTGATGC TAAAGCCTTG GTGCGCTCTG ACTTCCTTCT GGTGTATGGG 
GATGTCATCT CAAACATCAA TATTACCAGA GCCCTGGAAG AACACAGGTT AAGGCGGAAG 
CTAGAAAAAA ATGTATCTGT GATGACGATG ATCTTCAAGG AGTCATCCCC CAGCCACCCA 
ACTCGTTGTC ATGAGGATAA CGTGGTAGTG GCCGTGGATA GTGCCACAAA TCAGGTGCTC 
CATTTCCAGA AGACCCAAGG CCTCCGACGT TTTTCTTTCC CTCTGAGCTT GTTCCAGGGC 
AGTGGAGATG GAGTGGAGAT TCGCTATGAC TTACTTGATT GTCATATCAG CATCTGCTCT 
CCTCAGGTGG CTCAACTATT TACAGACAAC TTCGACTACC AAACACGAGA TGACTTTGTG 
CGAGGCCTGT TAGTAAATGA GGAGATCTTA GGAAACCAGA TCCACATGCA TGTGACAACT 
AAGGAATATG GTGCCCGGGT CTCCAACCTA CACATGTATG CTGCTGTCTG CGCGGATGTC 
ATCCGCCGAT GGGTCTACCC TCTCACCCCA GAGGCTAACT TCACTGACAG CACAACCCAG 
AGCTGCACTC ACTCCCGACA TAACATCTAC CGAGGGCCTG AGGTCAGCCT GGGCCATGGC 
AGCATCCTGG AGGAAAATGT GCTCTTAGGT TCTGGCACTG TCATTGGCAG CAATTGCTCC 
ATCACTAACA GTGTCATTGG CCCTGGCTGT CACATTGGTG ATAATGTGGT GCTGGACCAG 
GCCTTCCTAT GGCAGGGTGT TCGAGTGGCT GCTGGAGCAC AGATTCATCA GTCTCTGCTC 
TGTGACAATG CTGAGGTCAA GGAAAATGTT ACACTGAAGC CTCACTGTGT CCTCACTTCC 
CAGGTGGTAG TGGGCCCAGA TATCATGCTA CCTGAGGGCT CAGTGATCTC TTTGCACCCT 
CCAGATGCAG AGGATGATGA GGATGATGGT CAGTTCAGTG ATGATTCTGG GGCTGACCAA 
GAAAAAGAGA AAATGAAGCT GAAAGGTTAC AATCCAGCAG AAGTTGGAGT TGCAGGCCAG 
GGCTACCTCT GGAAAGCTGC AGATATGAAC ATGGAGGAAG AGGAGGAACT ACGGCAGAAT 
CTATGGGGAC TCAAAATCAA CATGGAAGAA GAGAGTGAAA CTGAAAGTGA GCGAAGTATG 
GATTCTGAAG AGCTAGACAG TCGGGCAGGC TCTCCACAGA TGGATGACAT CAAAGTGTTT 
CAGAATGAAG TCCTGGGAAC CCTGCAGCGG GGCAAGGAGG AAAACATTTC TTGTGACAAT 
CTAGTCCTGG AGATAAATTC TCTCAAGTAT GCCTACAACA TAAGCCTGAA GGAAGTGATG 
CAAGTACTGA GCCATGTGGT CCTGGAGTTC CCACTGCAAC AAATGGATTC CCCGCTAGAC 
CCAAACCGCT ATTGCACCCT GTTGATTCCT TTGCTCAAGG CCTGGAGCCC TGTCTTTAGG 
AACTACATAA AGCGCGCCGC TGATCATTTG GAATCTTTGG CAGCCATTGA GGACTTCTTT 
TTGGAGCATG AAGCTCTTAG CACTTCCATG GCCAAGGTAC TGATGGCTTT TTACCAACTG 
GAGATCCTGG CTGAGGAAAC AATTCTGAGC TGGTTCAGCC GAAGGGAGGT AACTGACAAA 
GGCCAGCAGT TGCGAAAGAA CCAGCAGCTG CAGAGGTTCA TCCAGTGGCT AAAAGAGGCA 
GAAGAGGAGT CATCTGAAGA TGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa02578
Clone ID Related Accession (Same CDS sequence) XM_001251995.5 , XM_002684878.3
Accession Version XM_001251995.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product translation initiation factor eIF-2B subunit epsilon
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001251995.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCA CTGTGGTGGC ACCGCCTGGT GCGTTGGCTA GTCGGGTCAA CAAGCGCAGC 
GGCGGCGGAC CGGGAGGCGG TGGCGGCGGT GGTGGCGGCG GCGGTGGTGG GGCAGCCAGA 
GGGGCGGAGG AGGAACCGCC GCCGCCCCTA CAAGCAGTTC TGGTGGCCGA CAGCTTCAAC 
CGCCGCTTCT TTCCCATCTC CAAGGACCAG CCTCGGGTCC TCTTGCCCCT GGCCAATGTG 
GCACTAATTG ACTACACTCT GGAATTTCTG ACTGCCACAG GTGTACAGGA AACCTTTGTC 
TTTTGTTGCT GGAAGGCTGC TCAAATCAAG GAACATTTAC TGAAATCCAA GTGGTGCCGC 
CCAACATCCC TCAATGTGGT TCGAATAATT ACATCAGAGC TGTATAGATC ACTGGGGGAT 
GTTCTCCGTG ATGTTGATGC TAAAGCCTTG GTGCGCTCTG ACTTCCTTCT GGTGTATGGG 
GATGTCATCT CAAACATCAA TATTACCAGA GCCCTGGAAG AACACAGGTT AAGGCGGAAG 
CTAGAAAAAA ATGTATCTGT GATGACGATG ATCTTCAAGG AGTCATCCCC CAGCCACCCA 
ACTCGTTGTC ATGAGGATAA CGTGGTAGTG GCCGTGGATA GTGCCACAAA TCAGGTGCTC 
CATTTCCAGA AGACCCAAGG CCTCCGACGT TTTTCTTTCC CTCTGAGCTT GTTCCAGGGC 
AGTGGAGATG GAGTGGAGAT TCGCTATGAC TTACTTGATT GTCATATCAG CATCTGCTCT 
CCTCAGGTGG CTCAACTATT TACAGACAAC TTCGACTACC AAACACGAGA TGACTTTGTG 
CGAGGCCTGT TAGTAAATGA GGAGATCTTA GGAAACCAGA TCCACATGCA TGTGACAACT 
AAGGAATATG GTGCCCGGGT CTCCAACCTA CACATGTATG CTGCTGTCTG CGCGGATGTC 
ATCCGCCGAT GGGTCTACCC TCTCACCCCA GAGGCTAACT TCACTGACAG CACAACCCAG 
AGCTGCACTC ACTCCCGACA TAACATCTAC CGAGGGCCTG AGGTCAGCCT GGGCCATGGC 
AGCATCCTGG AGGAAAATGT GCTCTTAGGT TCTGGCACTG TCATTGGCAG CAATTGCTCC 
ATCACTAACA GTGTCATTGG CCCTGGCTGT CACATTGGTG ATAATGTGGT GCTGGACCAG 
GCCTTCCTAT GGCAGGGTGT TCGAGTGGCT GCTGGAGCAC AGATTCATCA GTCTCTGCTC 
TGTGACAATG CTGAGGTCAA GGAAAATGTT ACACTGAAGC CTCACTGTGT CCTCACTTCC 
CAGGTGGTAG TGGGCCCAGA TATCATGCTA CCTGAGGGCT CAGTGATCTC TTTGCACCCT 
CCAGATGCAG AGGATGATGA GGATGATGGT CAGTTCAGTG ATGATTCTGG GGCTGACCAA 
GAAAAAGAGA AAATGAAGCT GAAAGGTTAC AATCCAGCAG AAGTTGGAGT TGCAGGCCAG 
GGCTACCTCT GGAAAGCTGC AGATATGAAC ATGAAGGAAG AGGAGGAACT ACGGCAGAAT 
CTATGGGGAC TCAAAATCAA CATGGAAGAA GAGAGTGAAA CTGAAAGTGA GCGAAGTATG 
GATTCTGAAG AGCTAGACAG TCGGGCAGGC TCTCCACAGA TGGATGACAT CAAAGTGTTT 
CAGAATGAAG TCCTGGGAAC CCTGCAGCGG GGCAAGGAGG AAAACATTTC TTGTGACAAT 
CTAGTCCTGG AGATAAATTC TCTCAAGTAT GCCTACAACA TAAGCCTGAA GGAAGTGATG 
CAAGTACTGA GCCATGTGGT CCTGGAGTTC CCACTGCAAC AAATGGATTC CCCGCTAGAC 
CCAAACCGCT ATTGCACCCT GTTGATTCCT TTGCTCAAGG CCTGGAGCCC TGTCTTTAGG 
AACTACATAA AGCGCGCCGC TGATCATTTG GAATCTTTGG CAGCCATTGA GGACTTCTTT 
TTGGAGCATG AAGCTCTTAG CACTTCCATG GCCAAGGTAC TGATGGCTTT TTACCAACTG 
GAGATCCTGG CTGAGGAAAC AATTCTGAGC TGGTTCAGCC GAAGGGAGGT AACTGACAAA 
GGCCAGCAGT TGCGAAAGAA CCAGCAGCTG CAGAGGTTCA TCCAGTGGCT AAAAGAGGCA 
GAAGAGGAGT CATCTGAAGA TGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_001251996.1
CDS27..2213
Translation

Target ORF information:

RefSeq Version XM_001251995.5
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_001251995.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCA CTGTGGTGGC ACCGCCTGGT GCGTTGGCTA GTCGGGTCAA CAAGCGCAGC 
GGCGGCGGAC CGGGAGGCGG TGGCGGCGGT GGTGGCGGCG GCGGTGGTGG GGCAGCCAGA 
GGGGCGGAGG AGGAACCGCC GCCGCCCCTA CAAGCAGTTC TGGTGGCCGA CAGCTTCAAC 
CGCCGCTTCT TTCCCATCTC CAAGGACCAG CCTCGGGTCC TCTTGCCCCT GGCCAATGTG 
GCACTAATTG ACTACACTCT GGAATTTCTG ACTGCCACAG GTGTACAGGA AACCTTTGTC 
TTTTGTTGCT GGAAGGCTGC TCAAATCAAG GAACATTTAC TGAAATCCAA GTGGTGCCGC 
CCAACATCCC TCAATGTGGT TCGAATAATT ACATCAGAGC TGTATAGATC ACTGGGGGAT 
GTTCTCCGTG ATGTTGATGC TAAAGCCTTG GTGCGCTCTG ACTTCCTTCT GGTGTATGGG 
GATGTCATCT CAAACATCAA TATTACCAGA GCCCTGGAAG AACACAGGTT AAGGCGGAAG 
CTAGAAAAAA ATGTATCTGT GATGACGATG ATCTTCAAGG AGTCATCCCC CAGCCACCCA 
ACTCGTTGTC ATGAGGATAA CGTGGTAGTG GCCGTGGATA GTGCCACAAA TCAGGTGCTC 
CATTTCCAGA AGACCCAAGG CCTCCGACGT TTTTCTTTCC CTCTGAGCTT GTTCCAGGGC 
AGTGGAGATG GAGTGGAGAT TCGCTATGAC TTACTTGATT GTCATATCAG CATCTGCTCT 
CCTCAGGTGG CTCAACTATT TACAGACAAC TTCGACTACC AAACACGAGA TGACTTTGTG 
CGAGGCCTGT TAGTAAATGA GGAGATCTTA GGAAACCAGA TCCACATGCA TGTGACAACT 
AAGGAATATG GTGCCCGGGT CTCCAACCTA CACATGTATG CTGCTGTCTG CGCGGATGTC 
ATCCGCCGAT GGGTCTACCC TCTCACCCCA GAGGCTAACT TCACTGACAG CACAACCCAG 
AGCTGCACTC ACTCCCGACA TAACATCTAC CGAGGGCCTG AGGTCAGCCT GGGCCATGGC 
AGCATCCTGG AGGAAAATGT GCTCTTAGGT TCTGGCACTG TCATTGGCAG CAATTGCTCC 
ATCACTAACA GTGTCATTGG CCCTGGCTGT CACATTGGTG ATAATGTGGT GCTGGACCAG 
GCCTTCCTAT GGCAGGGTGT TCGAGTGGCT GCTGGAGCAC AGATTCATCA GTCTCTGCTC 
TGTGACAATG CTGAGGTCAA GGAAAATGTT ACACTGAAGC CTCACTGTGT CCTCACTTCC 
CAGGTGGTAG TGGGCCCAGA TATCATGCTA CCTGAGGGCT CAGTGATCTC TTTGCACCCT 
CCAGATGCAG AGGATGATGA GGATGATGGT CAGTTCAGTG ATGATTCTGG GGCTGACCAA 
GAAAAAGAGA AAATGAAGCT GAAAGGTTAC AATCCAGCAG AAGTTGGAGT TGCAGGCCAG 
GGCTACCTCT GGAAAGCTGC AGATATGAAC ATGAAGGAAG AGGAGGAACT ACGGCAGAAT 
CTATGGGGAC TCAAAATCAA CATGGAAGAA GAGAGTGAAA CTGAAAGTGA GCGAAGTATG 
GATTCTGAAG AGCTAGACAG TCGGGCAGGC TCTCCACAGA TGGATGACAT CAAAGTGTTT 
CAGAATGAAG TCCTGGGAAC CCTGCAGCGG GGCAAGGAGG AAAACATTTC TTGTGACAAT 
CTAGTCCTGG AGATAAATTC TCTCAAGTAT GCCTACAACA TAAGCCTGAA GGAAGTGATG 
CAAGTACTGA GCCATGTGGT CCTGGAGTTC CCACTGCAAC AAATGGATTC CCCGCTAGAC 
CCAAACCGCT ATTGCACCCT GTTGATTCCT TTGCTCAAGG CCTGGAGCCC TGTCTTTAGG 
AACTACATAA AGCGCGCCGC TGATCATTTG GAATCTTTGG CAGCCATTGA GGACTTCTTT 
TTGGAGCATG AAGCTCTTAG CACTTCCATG GCCAAGGTAC TGATGGCTTT TTACCAACTG 
GAGATCCTGG CTGAGGAAAC AATTCTGAGC TGGTTCAGCC GAAGGGAGGT AACTGACAAA 
GGCCAGCAGT TGCGAAAGAA CCAGCAGCTG CAGAGGTTCA TCCAGTGGCT AAAAGAGGCA 
GAAGAGGAGT CATCTGAAGA TGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa02578
Clone ID Related Accession (Same CDS sequence) XM_001251995.5 , XM_002684878.3
Accession Version XM_002684878.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product translation initiation factor eIF-2B subunit epsilon
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002684878.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCA CTGTGGTGGC ACCGCCTGGT GCGTTGGCTA GTCGGGTCAA CAAGCGCAGC 
GGCGGCGGAC CGGGAGGCGG TGGCGGCGGT GGTGGCGGCG GCGGTGGTGG GGCAGCCAGA 
GGGGCGGAGG AGGAACCGCC GCCGCCCCTA CAAGCAGTTC TGGTGGCCGA CAGCTTCAAC 
CGCCGCTTCT TTCCCATCTC CAAGGACCAG CCTCGGGTCC TCTTGCCCCT GGCCAATGTG 
GCACTAATTG ACTACACTCT GGAATTTCTG ACTGCCACAG GTGTACAGGA AACCTTTGTC 
TTTTGTTGCT GGAAGGCTGC TCAAATCAAG GAACATTTAC TGAAATCCAA GTGGTGCCGC 
CCAACATCCC TCAATGTGGT TCGAATAATT ACATCAGAGC TGTATAGATC ACTGGGGGAT 
GTTCTCCGTG ATGTTGATGC TAAAGCCTTG GTGCGCTCTG ACTTCCTTCT GGTGTATGGG 
GATGTCATCT CAAACATCAA TATTACCAGA GCCCTGGAAG AACACAGGTT AAGGCGGAAG 
CTAGAAAAAA ATGTATCTGT GATGACGATG ATCTTCAAGG AGTCATCCCC CAGCCACCCA 
ACTCGTTGTC ATGAGGATAA CGTGGTAGTG GCCGTGGATA GTGCCACAAA TCAGGTGCTC 
CATTTCCAGA AGACCCAAGG CCTCCGACGT TTTTCTTTCC CTCTGAGCTT GTTCCAGGGC 
AGTGGAGATG GAGTGGAGAT TCGCTATGAC TTACTTGATT GTCATATCAG CATCTGCTCT 
CCTCAGGTGG CTCAACTATT TACAGACAAC TTCGACTACC AAACACGAGA TGACTTTGTG 
CGAGGCCTGT TAGTAAATGA GGAGATCTTA GGAAACCAGA TCCACATGCA TGTGACAACT 
AAGGAATATG GTGCCCGGGT CTCCAACCTA CACATGTATG CTGCTGTCTG CGCGGATGTC 
ATCCGCCGAT GGGTCTACCC TCTCACCCCA GAGGCTAACT TCACTGACAG CACAACCCAG 
AGCTGCACTC ACTCCCGACA TAACATCTAC CGAGGGCCTG AGGTCAGCCT GGGCCATGGC 
AGCATCCTGG AGGAAAATGT GCTCTTAGGT TCTGGCACTG TCATTGGCAG CAATTGCTCC 
ATCACTAACA GTGTCATTGG CCCTGGCTGT CACATTGGTG ATAATGTGGT GCTGGACCAG 
GCCTTCCTAT GGCAGGGTGT TCGAGTGGCT GCTGGAGCAC AGATTCATCA GTCTCTGCTC 
TGTGACAATG CTGAGGTCAA GGAAAATGTT ACACTGAAGC CTCACTGTGT CCTCACTTCC 
CAGGTGGTAG TGGGCCCAGA TATCATGCTA CCTGAGGGCT CAGTGATCTC TTTGCACCCT 
CCAGATGCAG AGGATGATGA GGATGATGGT CAGTTCAGTG ATGATTCTGG GGCTGACCAA 
GAAAAAGAGA AAATGAAGCT GAAAGGTTAC AATCCAGCAG AAGTTGGAGT TGCAGGCCAG 
GGCTACCTCT GGAAAGCTGC AGATATGAAC ATGAAGGAAG AGGAGGAACT ACGGCAGAAT 
CTATGGGGAC TCAAAATCAA CATGGAAGAA GAGAGTGAAA CTGAAAGTGA GCGAAGTATG 
GATTCTGAAG AGCTAGACAG TCGGGCAGGC TCTCCACAGA TGGATGACAT CAAAGTGTTT 
CAGAATGAAG TCCTGGGAAC CCTGCAGCGG GGCAAGGAGG AAAACATTTC TTGTGACAAT 
CTAGTCCTGG AGATAAATTC TCTCAAGTAT GCCTACAACA TAAGCCTGAA GGAAGTGATG 
CAAGTACTGA GCCATGTGGT CCTGGAGTTC CCACTGCAAC AAATGGATTC CCCGCTAGAC 
CCAAACCGCT ATTGCACCCT GTTGATTCCT TTGCTCAAGG CCTGGAGCCC TGTCTTTAGG 
AACTACATAA AGCGCGCCGC TGATCATTTG GAATCTTTGG CAGCCATTGA GGACTTCTTT 
TTGGAGCATG AAGCTCTTAG CACTTCCATG GCCAAGGTAC TGATGGCTTT TTACCAACTG 
GAGATCCTGG CTGAGGAAAC AATTCTGAGC TGGTTCAGCC GAAGGGAGGT AACTGACAAA 
GGCCAGCAGT TGCGAAAGAA CCAGCAGCTG CAGAGGTTCA TCCAGTGGCT AAAAGAGGCA 
GAAGAGGAGT CATCTGAAGA TGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002684924.1
CDS27..2213
Translation

Target ORF information:

RefSeq Version XM_002684878.3
Organism Bos taurus(cattle)
Definition Bos taurus eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002684878.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCA CTGTGGTGGC ACCGCCTGGT GCGTTGGCTA GTCGGGTCAA CAAGCGCAGC 
GGCGGCGGAC CGGGAGGCGG TGGCGGCGGT GGTGGCGGCG GCGGTGGTGG GGCAGCCAGA 
GGGGCGGAGG AGGAACCGCC GCCGCCCCTA CAAGCAGTTC TGGTGGCCGA CAGCTTCAAC 
CGCCGCTTCT TTCCCATCTC CAAGGACCAG CCTCGGGTCC TCTTGCCCCT GGCCAATGTG 
GCACTAATTG ACTACACTCT GGAATTTCTG ACTGCCACAG GTGTACAGGA AACCTTTGTC 
TTTTGTTGCT GGAAGGCTGC TCAAATCAAG GAACATTTAC TGAAATCCAA GTGGTGCCGC 
CCAACATCCC TCAATGTGGT TCGAATAATT ACATCAGAGC TGTATAGATC ACTGGGGGAT 
GTTCTCCGTG ATGTTGATGC TAAAGCCTTG GTGCGCTCTG ACTTCCTTCT GGTGTATGGG 
GATGTCATCT CAAACATCAA TATTACCAGA GCCCTGGAAG AACACAGGTT AAGGCGGAAG 
CTAGAAAAAA ATGTATCTGT GATGACGATG ATCTTCAAGG AGTCATCCCC CAGCCACCCA 
ACTCGTTGTC ATGAGGATAA CGTGGTAGTG GCCGTGGATA GTGCCACAAA TCAGGTGCTC 
CATTTCCAGA AGACCCAAGG CCTCCGACGT TTTTCTTTCC CTCTGAGCTT GTTCCAGGGC 
AGTGGAGATG GAGTGGAGAT TCGCTATGAC TTACTTGATT GTCATATCAG CATCTGCTCT 
CCTCAGGTGG CTCAACTATT TACAGACAAC TTCGACTACC AAACACGAGA TGACTTTGTG 
CGAGGCCTGT TAGTAAATGA GGAGATCTTA GGAAACCAGA TCCACATGCA TGTGACAACT 
AAGGAATATG GTGCCCGGGT CTCCAACCTA CACATGTATG CTGCTGTCTG CGCGGATGTC 
ATCCGCCGAT GGGTCTACCC TCTCACCCCA GAGGCTAACT TCACTGACAG CACAACCCAG 
AGCTGCACTC ACTCCCGACA TAACATCTAC CGAGGGCCTG AGGTCAGCCT GGGCCATGGC 
AGCATCCTGG AGGAAAATGT GCTCTTAGGT TCTGGCACTG TCATTGGCAG CAATTGCTCC 
ATCACTAACA GTGTCATTGG CCCTGGCTGT CACATTGGTG ATAATGTGGT GCTGGACCAG 
GCCTTCCTAT GGCAGGGTGT TCGAGTGGCT GCTGGAGCAC AGATTCATCA GTCTCTGCTC 
TGTGACAATG CTGAGGTCAA GGAAAATGTT ACACTGAAGC CTCACTGTGT CCTCACTTCC 
CAGGTGGTAG TGGGCCCAGA TATCATGCTA CCTGAGGGCT CAGTGATCTC TTTGCACCCT 
CCAGATGCAG AGGATGATGA GGATGATGGT CAGTTCAGTG ATGATTCTGG GGCTGACCAA 
GAAAAAGAGA AAATGAAGCT GAAAGGTTAC AATCCAGCAG AAGTTGGAGT TGCAGGCCAG 
GGCTACCTCT GGAAAGCTGC AGATATGAAC ATGAAGGAAG AGGAGGAACT ACGGCAGAAT 
CTATGGGGAC TCAAAATCAA CATGGAAGAA GAGAGTGAAA CTGAAAGTGA GCGAAGTATG 
GATTCTGAAG AGCTAGACAG TCGGGCAGGC TCTCCACAGA TGGATGACAT CAAAGTGTTT 
CAGAATGAAG TCCTGGGAAC CCTGCAGCGG GGCAAGGAGG AAAACATTTC TTGTGACAAT 
CTAGTCCTGG AGATAAATTC TCTCAAGTAT GCCTACAACA TAAGCCTGAA GGAAGTGATG 
CAAGTACTGA GCCATGTGGT CCTGGAGTTC CCACTGCAAC AAATGGATTC CCCGCTAGAC 
CCAAACCGCT ATTGCACCCT GTTGATTCCT TTGCTCAAGG CCTGGAGCCC TGTCTTTAGG 
AACTACATAA AGCGCGCCGC TGATCATTTG GAATCTTTGG CAGCCATTGA GGACTTCTTT 
TTGGAGCATG AAGCTCTTAG CACTTCCATG GCCAAGGTAC TGATGGCTTT TTACCAACTG 
GAGATCCTGG CTGAGGAAAC AATTCTGAGC TGGTTCAGCC GAAGGGAGGT AACTGACAAA 
GGCCAGCAGT TGCGAAAGAA CCAGCAGCTG CAGAGGTTCA TCCAGTGGCT AAAAGAGGCA 
GAAGAGGAGT CATCTGAAGA TGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed