agl-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	agl-1
Entrez Gene ID	175091
Full Name	AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_064583.5	NP_496984.1	AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme
NM_064583.6	NP_496984.1	AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Homo sapiens (human)	AGL	NP_000635.2
	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Agl1 deficient animals have reduced lifespan and dramatic increase of glucosylated oligomannose glycans.
Title: Deficiency of ?-glucosidase I alters glycoprotein glycosylation and lifespan in Caenorhabditis elegans.

The following agl-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agl-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337921	NM_064583.6 Latest version!	Caenorhabditis elegans AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme (agl-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OCa337921	NM_064583.5	Caenorhabditis elegans AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme (agl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agl-1 ( NM_064583.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496984.1 Caenorhabditis elegans AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme (agl-1), partial mRNA.

Price & Availability↑

CloneID	OCa337921
Clone ID Related Accession (Same CDS sequence)	NM_064583.5 , NM_064583.6
Accession Version	NM_064583.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_064583.4. COMPLETENESS: incomplete on both ends.

ATGGGTGAAG AAAAAGAAAT CCGAATCATT GTTCTTGAAG ATGGAGAGCA CTTGGAAAGT 
GTTATAAGAA AAATTGAGAA AGGTTGGATA GTTCGATTCA AGCGCGGCTC TTCACTTCTT 
GGAAAGAAAG TTAAAGTTTT CACCTCTATT TGCCCTGGAA ATTCTCTGGA ATGGTCTGAA 
GGAAAAGACC ACTTGGCAGT TTACTGTCAA GTGGAATGCA AGGAAGCTGG ATCCTTCAGA 
TATTGGTTTA AAATTGAGGA TTCTGAAGAG CGAGGCAGTG GATACTTCTT GGTGATGCCA 
GAGCTGAAGA TCAACGGAAA ATGCCTGCCG CTCGATGGAA TCGCTTGCCA AACGTACCTC 
ACAAAGCTTC TAGGACCGCT TTCCGAGTGG AAAGAACGAC TTGAAGTTGC ACATCAGACA 
GGATATAACA TGATCCATTT GACTCCTATC CATGAGCTTG GAATCTCAAA TAGTAGCTAT 
TCCCTCAGTG ATCATCATTC TTTGATTAAA ACCATCCAGT CGCAGGATCA AAAGTTCGGA 
TTTGAAGACG TGCAAGCGCT CGTGGGAGAT CTTGAGCGGT CCTGGAACAT ACTCACAGTA 
CAGGATGTTG TGTGGAATCA CGCAGCAAAG AATGCCACGT GGCTTCTGGA GCATCCAGAG 
TCTGCCTATA ACTGCTTGAA TAGTCCACAT CTTCGGCCAG CATATGTAAT TGACAGAGTG 
TATCACGAAT TTGGAAAGCA GATTGGAGAA GGTGTGTGGG AACATCGTGG TGTTCCACCA 
GTCGTTGACA ATATTCACCA TGTAAATGCA ATCGAGTATC TTCTGAGAGC AGAAGTTTTG 
CCAAAGGCCG ATCTTCACGA GTTTTATCAA GTGGATTTGA AGGCAATGGT TAACCTATTC 
GAGGTGTTCA TCAAACAATC TAGTGGTCCC ACAACGAACC CGTTGGACGG AGAAGATGTA 
GAAATCGAAC AAGATTTAGA ATGTCGAAGA TTCGGAAACA CTGTGGACTT TGAGAGATCC 
GCTAGAATTT TCAATCGACA CCGGGGAGAT GCTAAGTCAG AAGAAGAACG AGTGGAAAAA 
TGCGTTCGAA GCTTTGAAGA AGCCCTCAAT AACAAAAATC TCGAAGCCGC TCGCGAGTCA 
TGGGAAGTGA TTTTAGCCGG CTTGGCAGCA GTGATGGGAG GAATCACATA TGAGCGAATT 
GCTGATAACG GCCCGAAAAA GGGACTTGTA TCTCCGGAAA ACCCGTTGAC AACTGATTAT 
TTTCTGCATT TGGAGGCAGA TCTCGGGTGG AAATCAGAAG AGAAATTCGC ATATGATCCA 
GAAAAGTCAA AGTTTTTAAT GGCTTTTAAT GGATGGGTTA TGAGCAGTGA TCCGATGAAG 
AATTTTGCGC TGAAAGAATC TCAGGTTTAT CTCCGCCGTG AGCTCGTCTG CTGGGGTGAT 
TCCGTAAAGC TAAATTATGG AAACAAAGAA GCGGATAGCC CATTCCTCTG GCAATATATG 
AAGGAATACA CGCAACAAGC CGCCCGAATC TTCCACGGTC TCCGCATCGA CAACGCTCAT 
GGCACGCCGA TCCACGTGGC TGAAAAGTTA CTGAAAACGG CAAGAGAAGT CCGGCCGGAT 
ATCTATGTTT TCGCAGAATT GTTCACCGGA AGTGAGCATG CCGACAACAT GTTCGTGAAC 
AGATTAGGAA TCTCGTCTTT AATTCGAGAA GCTCAATCGG CACATGACAG TCACGAGCAG 
GGACGATTGG TTTATAGATA CGGAGGTGAC TGTGTAGGGG CGTTCAAGCA AAAATCGGCT 
CGCCTGGCTC CAAAATCGAT TGCTCACGGC TTATTCTTGG ACCAATCTCA TGACAATCCG 
TCACCAATTC ACACTAGAAG CCCATTCGAC ATTCTTCCAA CCGCCGCCAT GCTCACAATG 
GCTTCTTGTG CTGTAGGTAG TAATCGAGGT TACGACGAGC TCATTAGAGA TCACATCCAC 
GTGGTTTCCG AAAAACGCCC ATATGCTTCT TGGTGCCGTC CGGATCAAGT GTCTAGAAGC 
CAGGGAATCA TTGAAGGCCG GAATTTGCTC AACAAGCTTC ATACGTGGCT CGCCGAGCAC 
GGATATTCTC AAGTTTTTGT GGATCAAATG AATTCGGATA TTGTGGGAAT CACGCGGCAC 
AATCCACGAA CCCACGAAAC GGTCGTAGTA GTGTCCCATA CTTCATTTTC CAAGAATTAT 
ATCGATTGGC CCGGCGGTTT GAAGCATATT CCCATTGGTG GAGTGCTGGA AAACGTGATT 
TTCGAGATGA AATTGAAAAA GGTTCAAGAG GAATGGGGAA CTGAAGATCC AGATGTTCTC 
ATAGGACTTA AAAATTATGA GATGGAAATT CGAGAAAATG TGAATTTGGA CAATGGAACT 
ATGTTCAAGG TTCATGATGG ATACATTGAG CTCACGAATT TCCCAACTGG ATCAGTTGTT 
GGATTTAAAA TTCGACCATC TGATGAAGCA ACTAAAGCTT TTAATATGAT TCATAACTCG 
ATTACTCCAG AACAATCTGA ATTTGACTCT GCTCTCTCTC GTCTCACATA TCAATCATTC 
CCAAATCTTC TATTCCACTG TGAATCCGAG GACTATGCCA CGATACAACA AGGCGGATAC 
GATGTTCCGA ATTTCGGAAA ATTCGTCTAC TGCGGCCTTC AGGGCCTCGT GCCAGTCCTC 
GAAAAAATCC GCGATGATAA CGACCTCGGG CATCCATTGT GCCAAAATCT CCGTGATGGG 
ACGTGGATCT GCGACTATAT TGTCGGAAGA TTGGCGAAAT TCGAGAAGCT CGGAGAAGTT 
TCAGAGGCTA TCAGAAAATT TTTTGCACCG TTGGATCATG TGCCTTATTA CCTCCGTCCG 
TGCTATTTTG AACTACTCGT TTCTTATATT TACGGAAAAA TTCGTAAAGA GGCGTTGAAG 
AGAATGGCTC CGCAAATCTC CTCGTCATCC GCGTTAGTCC GACACTTGGC CATCTCAACT 
CTCTCATTCC TTGGATACAT TCCTGGAGCC GGTCTCGCAC CGATTCCGAC TTCCCTCCAA 
ATTGAAGATC AGTATCCATC AAGTTTGGCC GCCGGGCTAT CCCACTTTGC TGTTGGAATC 
TGGAGAAATT GGGGTCGTGA CACATTTATC GCCTTGCCGG GATGCCTTTT ATCAACTGGC 
CGATTCCAGG AAGCCCGTCA AATCATTCTC TCATTTGCCG GCGCTATTCG TCATGGACTC 
ATACCGAATC TTCTAGCCGA GGGAATTGGT GCCAGATATA ATTGTAGAGA TGCCACGTGG 
TTCTGGCTGG TTTCGATTGT GAAATACGTG GAATCGGCGC CAAATGGAGT CGGGATTCTT 
GAGGATCCAG TGCGCAGAAT CTACCCGAAT GATGATAGTG TCTATGGAGA GGGAGAAGTG 
CAACAGATGC TTATTGAAAC TATTTATGAG GCACTTGACA AGCATTTCGC TGGGATTGAC 
TACAGGGAGA GAAGTGCCGG ACCACAAATC GACGAACAAA TGAGAGATGA GGGATTCCAG 
GTGACCGCCG GCGTCTCTCG CACCACAGGC TTCATCTACG GCGGTAATCG CTGGAATTGT 
GGAACATGGA TGGACAAGAT GGGAAGTAGT GAAAGAGCCG GAAATAAAGG AGAACCCGCG 
ACGCCGAGAG ATGGAGCGGC CGTCGAGCTA CAGGGACTTG CCTACAGAGC ACTGAAAAGC 
TTGAAGAATT GGAAGGAGCA AGGTGTGATC CAGAGATCCG GAGTTAGCGA CGAGTGGACG 
TGGGGCTTCT GGGCTCAGAA GATCCGAGAA AACTTTGAGA AGGAGTTCTT TGTTGATAAA 
GATTCCTATG CGGAGTTTGT AAATCGTCGG GAGATCATCA AGGATTCTGT CGGTTCCACA 
CTAGGATTCA CCGACTTCCA GCTTCGTTGC AACTTTGGAA TTGCATTAGC AGTTGCTCCA 
GATCTTATGG ATCCTAAGAA AGCATGGAAG GCATTGGATT CAGCTGAAGT TCTTCTTGGA 
CCACTTGGAA TGAAGACCCT TGATCCAACA GATTGGGCTT ACAACGGCTA CTACAACAAT 
GATGATGATG GGACTGACAA GAAGACGGCA AAAGGATGGA ATTATCATCA GGGCCCTGAA 
TGGCTCTTCG TCGCCGGGTA TTATCTCCAA GCTCGGCTGA GAATCGGTGA TATCCTCGGA 
GGATCCGAAA AGCAGTATGC GATCCGGCAA GTCCAGGAGC GACTCGGCAA TGCTTACAAG 
CACATCATTT CATCACCATG GAGGTCACTA CCGGAACTGA CAAATGCTGA TGGAGAATAC 
TGTATGCAGA GTTGTGCTGC CCAGGCGTGG TCCGTCGGTT GCCTGATTGA AGCATGTGTA 
AAACTTAATA CTATCGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496984.1
CDS	1..4404
Translation	MGEEKEIRIIVLEDGEHLESVIRKIEKGWIVRFKRGSSLLGKKVKVFTSICPGNSLEWSEGKDHLAVYCQVECKEAGSFRYWFKIEDSEERGSGYFLVMPELKINGKCLPLDGIACQTYLTKLLGPLSEWKERLEVAHQTGYNMIHLTPIHELGISNSSYSLSDHHSLIKTIQSQDQKFGFEDVQALVGDLERSWNILTVQDVVWNHAAKNATWLLEHPESAYNCLNSPHLRPAYVIDRVYHEFGKQIGEGVWEHRGVPPVVDNIHHVNAIEYLLRAEVLPKADLHEFYQVDLKAMVNLFEVFIKQSSGPTTNPLDGEDVEIEQDLECRRFGNTVDFERSARIFNRHRGDAKSEEERVEKCVRSFEEALNNKNLEAARESWEVILAGLAAVMGGITYERIADNGPKKGLVSPENPLTTDYFLHLEADLGWKSEEKFAYDPEKSKFLMAFNGWVMSSDPMKNFALKESQVYLRRELVCWGDSVKLNYGNKEADSPFLWQYMKEYTQQAARIFHGLRIDNAHGTPIHVAEKLLKTAREVRPDIYVFAELFTGSEHADNMFVNRLGISSLIREAQSAHDSHEQGRLVYRYGGDCVGAFKQKSARLAPKSIAHGLFLDQSHDNPSPIHTRSPFDILPTAAMLTMASCAVGSNRGYDELIRDHIHVVSEKRPYASWCRPDQVSRSQGIIEGRNLLNKLHTWLAEHGYSQVFVDQMNSDIVGITRHNPRTHETVVVVSHTSFSKNYIDWPGGLKHIPIGGVLENVIFEMKLKKVQEEWGTEDPDVLIGLKNYEMEIRENVNLDNGTMFKVHDGYIELTNFPTGSVVGFKIRPSDEATKAFNMIHNSITPEQSEFDSALSRLTYQSFPNLLFHCESEDYATIQQGGYDVPNFGKFVYCGLQGLVPVLEKIRDDNDLGHPLCQNLRDGTWICDYIVGRLAKFEKLGEVSEAIRKFFAPLDHVPYYLRPCYFELLVSYIYGKIRKEALKRMAPQISSSSALVRHLAISTLSFLGYIPGAGLAPIPTSLQIEDQYPSSLAAGLSHFAVGIWRNWGRDTFIALPGCLLSTGRFQEARQIILSFAGAIRHGLIPNLLAEGIGARYNCRDATWFWLVSIVKYVESAPNGVGILEDPVRRIYPNDDSVYGEGEVQQMLIETIYEALDKHFAGIDYRERSAGPQIDEQMRDEGFQVTAGVSRTTGFIYGGNRWNCGTWMDKMGSSERAGNKGEPATPRDGAAVELQGLAYRALKSLKNWKEQGVIQRSGVSDEWTWGFWAQKIRENFEKEFFVDKDSYAEFVNRREIIKDSVGSTLGFTDFQLRCNFGIALAVAPDLMDPKKAWKALDSAEVLLGPLGMKTLDPTDWAYNGYYNNDDDGTDKKTAKGWNYHQGPEWLFVAGYYLQARLRIGDILGGSEKQYAIRQVQERLGNAYKHIISSPWRSLPELTNADGEYCMQSCAAQAWSVGCLIEACVKLNTIEG

Target ORF information:

RefSeq Version	NM_064583.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme (agl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064583.5

ORF Insert Sequence:

ATGGGTGAAG AAAAAGAAAT CCGAATCATT GTTCTTGAAG ATGGAGAGCA CTTGGAAAGT 
GTTATAAGAA AAATTGAGAA AGGTTGGATA GTTCGATTCA AGCGCGGCTC TTCACTTCTT 
GGAAAGAAAG TTAAAGTTTT CACCTCTATT TGCCCTGGAA ATTCTCTGGA ATGGTCTGAA 
GGAAAAGACC ACTTGGCAGT TTACTGTCAA GTGGAATGCA AGGAAGCTGG ATCCTTCAGA 
TATTGGTTTA AAATTGAGGA TTCTGAAGAG CGAGGCAGTG GATACTTCTT GGTGATGCCA 
GAGCTGAAGA TCAACGGAAA ATGCCTGCCG CTCGATGGAA TCGCTTGCCA AACGTACCTC 
ACAAAGCTTC TAGGACCGCT TTCCGAGTGG AAAGAACGAC TTGAAGTTGC ACATCAGACA 
GGATATAACA TGATCCATTT GACTCCTATC CATGAGCTTG GAATCTCAAA TAGTAGCTAT 
TCCCTCAGTG ATCATCATTC TTTGATTAAA ACCATCCAGT CGCAGGATCA AAAGTTCGGA 
TTTGAAGACG TGCAAGCGCT CGTGGGAGAT CTTGAGCGGT CCTGGAACAT ACTCACAGTA 
CAGGATGTTG TGTGGAATCA CGCAGCAAAG AATGCCACGT GGCTTCTGGA GCATCCAGAG 
TCTGCCTATA ACTGCTTGAA TAGTCCACAT CTTCGGCCAG CATATGTAAT TGACAGAGTG 
TATCACGAAT TTGGAAAGCA GATTGGAGAA GGTGTGTGGG AACATCGTGG TGTTCCACCA 
GTCGTTGACA ATATTCACCA TGTAAATGCA ATCGAGTATC TTCTGAGAGC AGAAGTTTTG 
CCAAAGGCCG ATCTTCACGA GTTTTATCAA GTGGATTTGA AGGCAATGGT TAACCTATTC 
GAGGTGTTCA TCAAACAATC TAGTGGTCCC ACAACGAACC CGTTGGACGG AGAAGATGTA 
GAAATCGAAC AAGATTTAGA ATGTCGAAGA TTCGGAAACA CTGTGGACTT TGAGAGATCC 
GCTAGAATTT TCAATCGACA CCGGGGAGAT GCTAAGTCAG AAGAAGAACG AGTGGAAAAA 
TGCGTTCGAA GCTTTGAAGA AGCCCTCAAT AACAAAAATC TCGAAGCCGC TCGCGAGTCA 
TGGGAAGTGA TTTTAGCCGG CTTGGCAGCA GTGATGGGAG GAATCACATA TGAGCGAATT 
GCTGATAACG GCCCGAAAAA GGGACTTGTA TCTCCGGAAA ACCCGTTGAC AACTGATTAT 
TTTCTGCATT TGGAGGCAGA TCTCGGGTGG AAATCAGAAG AGAAATTCGC ATATGATCCA 
GAAAAGTCAA AGTTTTTAAT GGCTTTTAAT GGATGGGTTA TGAGCAGTGA TCCGATGAAG 
AATTTTGCGC TGAAAGAATC TCAGGTTTAT CTCCGCCGTG AGCTCGTCTG CTGGGGTGAT 
TCCGTAAAGC TAAATTATGG AAACAAAGAA GCGGATAGCC CATTCCTCTG GCAATATATG 
AAGGAATACA CGCAACAAGC CGCCCGAATC TTCCACGGTC TCCGCATCGA CAACGCTCAT 
GGCACGCCGA TCCACGTGGC TGAAAAGTTA CTGAAAACGG CAAGAGAAGT CCGGCCGGAT 
ATCTATGTTT TCGCAGAATT GTTCACCGGA AGTGAGCATG CCGACAACAT GTTCGTGAAC 
AGATTAGGAA TCTCGTCTTT AATTCGAGAA GCTCAATCGG CACATGACAG TCACGAGCAG 
GGACGATTGG TTTATAGATA CGGAGGTGAC TGTGTAGGGG CGTTCAAGCA AAAATCGGCT 
CGCCTGGCTC CAAAATCGAT TGCTCACGGC TTATTCTTGG ACCAATCTCA TGACAATCCG 
TCACCAATTC ACACTAGAAG CCCATTCGAC ATTCTTCCAA CCGCCGCCAT GCTCACAATG 
GCTTCTTGTG CTGTAGGTAG TAATCGAGGT TACGACGAGC TCATTAGAGA TCACATCCAC 
GTGGTTTCCG AAAAACGCCC ATATGCTTCT TGGTGCCGTC CGGATCAAGT GTCTAGAAGC 
CAGGGAATCA TTGAAGGCCG GAATTTGCTC AACAAGCTTC ATACGTGGCT CGCCGAGCAC 
GGATATTCTC AAGTTTTTGT GGATCAAATG AATTCGGATA TTGTGGGAAT CACGCGGCAC 
AATCCACGAA CCCACGAAAC GGTCGTAGTA GTGTCCCATA CTTCATTTTC CAAGAATTAT 
ATCGATTGGC CCGGCGGTTT GAAGCATATT CCCATTGGTG GAGTGCTGGA AAACGTGATT 
TTCGAGATGA AATTGAAAAA GGTTCAAGAG GAATGGGGAA CTGAAGATCC AGATGTTCTC 
ATAGGACTTA AAAATTATGA GATGGAAATT CGAGAAAATG TGAATTTGGA CAATGGAACT 
ATGTTCAAGG TTCATGATGG ATACATTGAG CTCACGAATT TCCCAACTGG ATCAGTTGTT 
GGATTTAAAA TTCGACCATC TGATGAAGCA ACTAAAGCTT TTAATATGAT TCATAACTCG 
ATTACTCCAG AACAATCTGA ATTTGACTCT GCTCTCTCTC GTCTCACATA TCAATCATTC 
CCAAATCTTC TATTCCACTG TGAATCCGAG GACTATGCCA CGATACAACA AGGCGGATAC 
GATGTTCCGA ATTTCGGAAA ATTCGTCTAC TGCGGCCTTC AGGGCCTCGT GCCAGTCCTC 
GAAAAAATCC GCGATGATAA CGACCTCGGG CATCCATTGT GCCAAAATCT CCGTGATGGG 
ACGTGGATCT GCGACTATAT TGTCGGAAGA TTGGCGAAAT TCGAGAAGCT CGGAGAAGTT 
TCAGAGGCTA TCAGAAAATT TTTTGCACCG TTGGATCATG TGCCTTATTA CCTCCGTCCG 
TGCTATTTTG AACTACTCGT TTCTTATATT TACGGAAAAA TTCGTAAAGA GGCGTTGAAG 
AGAATGGCTC CGCAAATCTC CTCGTCATCC GCGTTAGTCC GACACTTGGC CATCTCAACT 
CTCTCATTCC TTGGATACAT TCCTGGAGCC GGTCTCGCAC CGATTCCGAC TTCCCTCCAA 
ATTGAAGATC AGTATCCATC AAGTTTGGCC GCCGGGCTAT CCCACTTTGC TGTTGGAATC 
TGGAGAAATT GGGGTCGTGA CACATTTATC GCCTTGCCGG GATGCCTTTT ATCAACTGGC 
CGATTCCAGG AAGCCCGTCA AATCATTCTC TCATTTGCCG GCGCTATTCG TCATGGACTC 
ATACCGAATC TTCTAGCCGA GGGAATTGGT GCCAGATATA ATTGTAGAGA TGCCACGTGG 
TTCTGGCTGG TTTCGATTGT GAAATACGTG GAATCGGCGC CAAATGGAGT CGGGATTCTT 
GAGGATCCAG TGCGCAGAAT CTACCCGAAT GATGATAGTG TCTATGGAGA GGGAGAAGTG 
CAACAGATGC TTATTGAAAC TATTTATGAG GCACTTGACA AGCATTTCGC TGGGATTGAC 
TACAGGGAGA GAAGTGCCGG ACCACAAATC GACGAACAAA TGAGAGATGA GGGATTCCAG 
GTGACCGCCG GCGTCTCTCG CACCACAGGC TTCATCTACG GCGGTAATCG CTGGAATTGT 
GGAACATGGA TGGACAAGAT GGGAAGTAGT GAAAGAGCCG GAAATAAAGG AGAACCCGCG 
ACGCCGAGAG ATGGAGCGGC CGTCGAGCTA CAGGGACTTG CCTACAGAGC ACTGAAAAGC 
TTGAAGAATT GGAAGGAGCA AGGTGTGATC CAGAGATCCG GAGTTAGCGA CGAGTGGACG 
TGGGGCTTCT GGGCTCAGAA GATCCGAGAA AACTTTGAGA AGGAGTTCTT TGTTGATAAA 
GATTCCTATG CGGAGTTTGT AAATCGTCGG GAGATCATCA AGGATTCTGT CGGTTCCACA 
CTAGGATTCA CCGACTTCCA GCTTCGTTGC AACTTTGGAA TTGCATTAGC AGTTGCTCCA 
GATCTTATGG ATCCTAAGAA AGCATGGAAG GCATTGGATT CAGCTGAAGT TCTTCTTGGA 
CCACTTGGAA TGAAGACCCT TGATCCAACA GATTGGGCTT ACAACGGCTA CTACAACAAT 
GATGATGATG GGACTGACAA GAAGACGGCA AAAGGATGGA ATTATCATCA GGGCCCTGAA 
TGGCTCTTCG TCGCCGGGTA TTATCTCCAA GCTCGGCTGA GAATCGGTGA TATCCTCGGA 
GGATCCGAAA AGCAGTATGC GATCCGGCAA GTCCAGGAGC GACTCGGCAA TGCTTACAAG 
CACATCATTT CATCACCATG GAGGTCACTA CCGGAACTGA CAAATGCTGA TGGAGAATAC 
TGTATGCAGA GTTGTGCTGC CCAGGCGTGG TCCGTCGGTT GCCTGATTGA AGCATGTGTA 
AAACTTAATA CTATCGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl-1 ( NM_064583.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496984.1 Caenorhabditis elegans AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme (agl-1), mRNA.

Price & Availability↑

CloneID	OCa337921
Clone ID Related Accession (Same CDS sequence)	NM_064583.5 , NM_064583.6
Accession Version	NM_064583.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_064583.5.

ATGGGTGAAG AAAAAGAAAT CCGAATCATT GTTCTTGAAG ATGGAGAGCA CTTGGAAAGT 
GTTATAAGAA AAATTGAGAA AGGTTGGATA GTTCGATTCA AGCGCGGCTC TTCACTTCTT 
GGAAAGAAAG TTAAAGTTTT CACCTCTATT TGCCCTGGAA ATTCTCTGGA ATGGTCTGAA 
GGAAAAGACC ACTTGGCAGT TTACTGTCAA GTGGAATGCA AGGAAGCTGG ATCCTTCAGA 
TATTGGTTTA AAATTGAGGA TTCTGAAGAG CGAGGCAGTG GATACTTCTT GGTGATGCCA 
GAGCTGAAGA TCAACGGAAA ATGCCTGCCG CTCGATGGAA TCGCTTGCCA AACGTACCTC 
ACAAAGCTTC TAGGACCGCT TTCCGAGTGG AAAGAACGAC TTGAAGTTGC ACATCAGACA 
GGATATAACA TGATCCATTT GACTCCTATC CATGAGCTTG GAATCTCAAA TAGTAGCTAT 
TCCCTCAGTG ATCATCATTC TTTGATTAAA ACCATCCAGT CGCAGGATCA AAAGTTCGGA 
TTTGAAGACG TGCAAGCGCT CGTGGGAGAT CTTGAGCGGT CCTGGAACAT ACTCACAGTA 
CAGGATGTTG TGTGGAATCA CGCAGCAAAG AATGCCACGT GGCTTCTGGA GCATCCAGAG 
TCTGCCTATA ACTGCTTGAA TAGTCCACAT CTTCGGCCAG CATATGTAAT TGACAGAGTG 
TATCACGAAT TTGGAAAGCA GATTGGAGAA GGTGTGTGGG AACATCGTGG TGTTCCACCA 
GTCGTTGACA ATATTCACCA TGTAAATGCA ATCGAGTATC TTCTGAGAGC AGAAGTTTTG 
CCAAAGGCCG ATCTTCACGA GTTTTATCAA GTGGATTTGA AGGCAATGGT TAACCTATTC 
GAGGTGTTCA TCAAACAATC TAGTGGTCCC ACAACGAACC CGTTGGACGG AGAAGATGTA 
GAAATCGAAC AAGATTTAGA ATGTCGAAGA TTCGGAAACA CTGTGGACTT TGAGAGATCC 
GCTAGAATTT TCAATCGACA CCGGGGAGAT GCTAAGTCAG AAGAAGAACG AGTGGAAAAA 
TGCGTTCGAA GCTTTGAAGA AGCCCTCAAT AACAAAAATC TCGAAGCCGC TCGCGAGTCA 
TGGGAAGTGA TTTTAGCCGG CTTGGCAGCA GTGATGGGAG GAATCACATA TGAGCGAATT 
GCTGATAACG GCCCGAAAAA GGGACTTGTA TCTCCGGAAA ACCCGTTGAC AACTGATTAT 
TTTCTGCATT TGGAGGCAGA TCTCGGGTGG AAATCAGAAG AGAAATTCGC ATATGATCCA 
GAAAAGTCAA AGTTTTTAAT GGCTTTTAAT GGATGGGTTA TGAGCAGTGA TCCGATGAAG 
AATTTTGCGC TGAAAGAATC TCAGGTTTAT CTCCGCCGTG AGCTCGTCTG CTGGGGTGAT 
TCCGTAAAGC TAAATTATGG AAACAAAGAA GCGGATAGCC CATTCCTCTG GCAATATATG 
AAGGAATACA CGCAACAAGC CGCCCGAATC TTCCACGGTC TCCGCATCGA CAACGCTCAT 
GGCACGCCGA TCCACGTGGC TGAAAAGTTA CTGAAAACGG CAAGAGAAGT CCGGCCGGAT 
ATCTATGTTT TCGCAGAATT GTTCACCGGA AGTGAGCATG CCGACAACAT GTTCGTGAAC 
AGATTAGGAA TCTCGTCTTT AATTCGAGAA GCTCAATCGG CACATGACAG TCACGAGCAG 
GGACGATTGG TTTATAGATA CGGAGGTGAC TGTGTAGGGG CGTTCAAGCA AAAATCGGCT 
CGCCTGGCTC CAAAATCGAT TGCTCACGGC TTATTCTTGG ACCAATCTCA TGACAATCCG 
TCACCAATTC ACACTAGAAG CCCATTCGAC ATTCTTCCAA CCGCCGCCAT GCTCACAATG 
GCTTCTTGTG CTGTAGGTAG TAATCGAGGT TACGACGAGC TCATTAGAGA TCACATCCAC 
GTGGTTTCCG AAAAACGCCC ATATGCTTCT TGGTGCCGTC CGGATCAAGT GTCTAGAAGC 
CAGGGAATCA TTGAAGGCCG GAATTTGCTC AACAAGCTTC ATACGTGGCT CGCCGAGCAC 
GGATATTCTC AAGTTTTTGT GGATCAAATG AATTCGGATA TTGTGGGAAT CACGCGGCAC 
AATCCACGAA CCCACGAAAC GGTCGTAGTA GTGTCCCATA CTTCATTTTC CAAGAATTAT 
ATCGATTGGC CCGGCGGTTT GAAGCATATT CCCATTGGTG GAGTGCTGGA AAACGTGATT 
TTCGAGATGA AATTGAAAAA GGTTCAAGAG GAATGGGGAA CTGAAGATCC AGATGTTCTC 
ATAGGACTTA AAAATTATGA GATGGAAATT CGAGAAAATG TGAATTTGGA CAATGGAACT 
ATGTTCAAGG TTCATGATGG ATACATTGAG CTCACGAATT TCCCAACTGG ATCAGTTGTT 
GGATTTAAAA TTCGACCATC TGATGAAGCA ACTAAAGCTT TTAATATGAT TCATAACTCG 
ATTACTCCAG AACAATCTGA ATTTGACTCT GCTCTCTCTC GTCTCACATA TCAATCATTC 
CCAAATCTTC TATTCCACTG TGAATCCGAG GACTATGCCA CGATACAACA AGGCGGATAC 
GATGTTCCGA ATTTCGGAAA ATTCGTCTAC TGCGGCCTTC AGGGCCTCGT GCCAGTCCTC 
GAAAAAATCC GCGATGATAA CGACCTCGGG CATCCATTGT GCCAAAATCT CCGTGATGGG 
ACGTGGATCT GCGACTATAT TGTCGGAAGA TTGGCGAAAT TCGAGAAGCT CGGAGAAGTT 
TCAGAGGCTA TCAGAAAATT TTTTGCACCG TTGGATCATG TGCCTTATTA CCTCCGTCCG 
TGCTATTTTG AACTACTCGT TTCTTATATT TACGGAAAAA TTCGTAAAGA GGCGTTGAAG 
AGAATGGCTC CGCAAATCTC CTCGTCATCC GCGTTAGTCC GACACTTGGC CATCTCAACT 
CTCTCATTCC TTGGATACAT TCCTGGAGCC GGTCTCGCAC CGATTCCGAC TTCCCTCCAA 
ATTGAAGATC AGTATCCATC AAGTTTGGCC GCCGGGCTAT CCCACTTTGC TGTTGGAATC 
TGGAGAAATT GGGGTCGTGA CACATTTATC GCCTTGCCGG GATGCCTTTT ATCAACTGGC 
CGATTCCAGG AAGCCCGTCA AATCATTCTC TCATTTGCCG GCGCTATTCG TCATGGACTC 
ATACCGAATC TTCTAGCCGA GGGAATTGGT GCCAGATATA ATTGTAGAGA TGCCACGTGG 
TTCTGGCTGG TTTCGATTGT GAAATACGTG GAATCGGCGC CAAATGGAGT CGGGATTCTT 
GAGGATCCAG TGCGCAGAAT CTACCCGAAT GATGATAGTG TCTATGGAGA GGGAGAAGTG 
CAACAGATGC TTATTGAAAC TATTTATGAG GCACTTGACA AGCATTTCGC TGGGATTGAC 
TACAGGGAGA GAAGTGCCGG ACCACAAATC GACGAACAAA TGAGAGATGA GGGATTCCAG 
GTGACCGCCG GCGTCTCTCG CACCACAGGC TTCATCTACG GCGGTAATCG CTGGAATTGT 
GGAACATGGA TGGACAAGAT GGGAAGTAGT GAAAGAGCCG GAAATAAAGG AGAACCCGCG 
ACGCCGAGAG ATGGAGCGGC CGTCGAGCTA CAGGGACTTG CCTACAGAGC ACTGAAAAGC 
TTGAAGAATT GGAAGGAGCA AGGTGTGATC CAGAGATCCG GAGTTAGCGA CGAGTGGACG 
TGGGGCTTCT GGGCTCAGAA GATCCGAGAA AACTTTGAGA AGGAGTTCTT TGTTGATAAA 
GATTCCTATG CGGAGTTTGT AAATCGTCGG GAGATCATCA AGGATTCTGT CGGTTCCACA 
CTAGGATTCA CCGACTTCCA GCTTCGTTGC AACTTTGGAA TTGCATTAGC AGTTGCTCCA 
GATCTTATGG ATCCTAAGAA AGCATGGAAG GCATTGGATT CAGCTGAAGT TCTTCTTGGA 
CCACTTGGAA TGAAGACCCT TGATCCAACA GATTGGGCTT ACAACGGCTA CTACAACAAT 
GATGATGATG GGACTGACAA GAAGACGGCA AAAGGATGGA ATTATCATCA GGGCCCTGAA 
TGGCTCTTCG TCGCCGGGTA TTATCTCCAA GCTCGGCTGA GAATCGGTGA TATCCTCGGA 
GGATCCGAAA AGCAGTATGC GATCCGGCAA GTCCAGGAGC GACTCGGCAA TGCTTACAAG 
CACATCATTT CATCACCATG GAGGTCACTA CCGGAACTGA CAAATGCTGA TGGAGAATAC 
TGTATGCAGA GTTGTGCTGC CCAGGCGTGG TCCGTCGGTT GCCTGATTGA AGCATGTGTA 
AAACTTAATA CTATCGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496984.1
CDS	190..4593
Translation	MGEEKEIRIIVLEDGEHLESVIRKIEKGWIVRFKRGSSLLGKKVKVFTSICPGNSLEWSEGKDHLAVYCQVECKEAGSFRYWFKIEDSEERGSGYFLVMPELKINGKCLPLDGIACQTYLTKLLGPLSEWKERLEVAHQTGYNMIHLTPIHELGISNSSYSLSDHHSLIKTIQSQDQKFGFEDVQALVGDLERSWNILTVQDVVWNHAAKNATWLLEHPESAYNCLNSPHLRPAYVIDRVYHEFGKQIGEGVWEHRGVPPVVDNIHHVNAIEYLLRAEVLPKADLHEFYQVDLKAMVNLFEVFIKQSSGPTTNPLDGEDVEIEQDLECRRFGNTVDFERSARIFNRHRGDAKSEEERVEKCVRSFEEALNNKNLEAARESWEVILAGLAAVMGGITYERIADNGPKKGLVSPENPLTTDYFLHLEADLGWKSEEKFAYDPEKSKFLMAFNGWVMSSDPMKNFALKESQVYLRRELVCWGDSVKLNYGNKEADSPFLWQYMKEYTQQAARIFHGLRIDNAHGTPIHVAEKLLKTAREVRPDIYVFAELFTGSEHADNMFVNRLGISSLIREAQSAHDSHEQGRLVYRYGGDCVGAFKQKSARLAPKSIAHGLFLDQSHDNPSPIHTRSPFDILPTAAMLTMASCAVGSNRGYDELIRDHIHVVSEKRPYASWCRPDQVSRSQGIIEGRNLLNKLHTWLAEHGYSQVFVDQMNSDIVGITRHNPRTHETVVVVSHTSFSKNYIDWPGGLKHIPIGGVLENVIFEMKLKKVQEEWGTEDPDVLIGLKNYEMEIRENVNLDNGTMFKVHDGYIELTNFPTGSVVGFKIRPSDEATKAFNMIHNSITPEQSEFDSALSRLTYQSFPNLLFHCESEDYATIQQGGYDVPNFGKFVYCGLQGLVPVLEKIRDDNDLGHPLCQNLRDGTWICDYIVGRLAKFEKLGEVSEAIRKFFAPLDHVPYYLRPCYFELLVSYIYGKIRKEALKRMAPQISSSSALVRHLAISTLSFLGYIPGAGLAPIPTSLQIEDQYPSSLAAGLSHFAVGIWRNWGRDTFIALPGCLLSTGRFQEARQIILSFAGAIRHGLIPNLLAEGIGARYNCRDATWFWLVSIVKYVESAPNGVGILEDPVRRIYPNDDSVYGEGEVQQMLIETIYEALDKHFAGIDYRERSAGPQIDEQMRDEGFQVTAGVSRTTGFIYGGNRWNCGTWMDKMGSSERAGNKGEPATPRDGAAVELQGLAYRALKSLKNWKEQGVIQRSGVSDEWTWGFWAQKIRENFEKEFFVDKDSYAEFVNRREIIKDSVGSTLGFTDFQLRCNFGIALAVAPDLMDPKKAWKALDSAEVLLGPLGMKTLDPTDWAYNGYYNNDDDGTDKKTAKGWNYHQGPEWLFVAGYYLQARLRIGDILGGSEKQYAIRQVQERLGNAYKHIISSPWRSLPELTNADGEYCMQSCAAQAWSVGCLIEACVKLNTIEG

Target ORF information:

RefSeq Version	NM_064583.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme (agl-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064583.6

ORF Insert Sequence:

ATGGGTGAAG AAAAAGAAAT CCGAATCATT GTTCTTGAAG ATGGAGAGCA CTTGGAAAGT 
GTTATAAGAA AAATTGAGAA AGGTTGGATA GTTCGATTCA AGCGCGGCTC TTCACTTCTT 
GGAAAGAAAG TTAAAGTTTT CACCTCTATT TGCCCTGGAA ATTCTCTGGA ATGGTCTGAA 
GGAAAAGACC ACTTGGCAGT TTACTGTCAA GTGGAATGCA AGGAAGCTGG ATCCTTCAGA 
TATTGGTTTA AAATTGAGGA TTCTGAAGAG CGAGGCAGTG GATACTTCTT GGTGATGCCA 
GAGCTGAAGA TCAACGGAAA ATGCCTGCCG CTCGATGGAA TCGCTTGCCA AACGTACCTC 
ACAAAGCTTC TAGGACCGCT TTCCGAGTGG AAAGAACGAC TTGAAGTTGC ACATCAGACA 
GGATATAACA TGATCCATTT GACTCCTATC CATGAGCTTG GAATCTCAAA TAGTAGCTAT 
TCCCTCAGTG ATCATCATTC TTTGATTAAA ACCATCCAGT CGCAGGATCA AAAGTTCGGA 
TTTGAAGACG TGCAAGCGCT CGTGGGAGAT CTTGAGCGGT CCTGGAACAT ACTCACAGTA 
CAGGATGTTG TGTGGAATCA CGCAGCAAAG AATGCCACGT GGCTTCTGGA GCATCCAGAG 
TCTGCCTATA ACTGCTTGAA TAGTCCACAT CTTCGGCCAG CATATGTAAT TGACAGAGTG 
TATCACGAAT TTGGAAAGCA GATTGGAGAA GGTGTGTGGG AACATCGTGG TGTTCCACCA 
GTCGTTGACA ATATTCACCA TGTAAATGCA ATCGAGTATC TTCTGAGAGC AGAAGTTTTG 
CCAAAGGCCG ATCTTCACGA GTTTTATCAA GTGGATTTGA AGGCAATGGT TAACCTATTC 
GAGGTGTTCA TCAAACAATC TAGTGGTCCC ACAACGAACC CGTTGGACGG AGAAGATGTA 
GAAATCGAAC AAGATTTAGA ATGTCGAAGA TTCGGAAACA CTGTGGACTT TGAGAGATCC 
GCTAGAATTT TCAATCGACA CCGGGGAGAT GCTAAGTCAG AAGAAGAACG AGTGGAAAAA 
TGCGTTCGAA GCTTTGAAGA AGCCCTCAAT AACAAAAATC TCGAAGCCGC TCGCGAGTCA 
TGGGAAGTGA TTTTAGCCGG CTTGGCAGCA GTGATGGGAG GAATCACATA TGAGCGAATT 
GCTGATAACG GCCCGAAAAA GGGACTTGTA TCTCCGGAAA ACCCGTTGAC AACTGATTAT 
TTTCTGCATT TGGAGGCAGA TCTCGGGTGG AAATCAGAAG AGAAATTCGC ATATGATCCA 
GAAAAGTCAA AGTTTTTAAT GGCTTTTAAT GGATGGGTTA TGAGCAGTGA TCCGATGAAG 
AATTTTGCGC TGAAAGAATC TCAGGTTTAT CTCCGCCGTG AGCTCGTCTG CTGGGGTGAT 
TCCGTAAAGC TAAATTATGG AAACAAAGAA GCGGATAGCC CATTCCTCTG GCAATATATG 
AAGGAATACA CGCAACAAGC CGCCCGAATC TTCCACGGTC TCCGCATCGA CAACGCTCAT 
GGCACGCCGA TCCACGTGGC TGAAAAGTTA CTGAAAACGG CAAGAGAAGT CCGGCCGGAT 
ATCTATGTTT TCGCAGAATT GTTCACCGGA AGTGAGCATG CCGACAACAT GTTCGTGAAC 
AGATTAGGAA TCTCGTCTTT AATTCGAGAA GCTCAATCGG CACATGACAG TCACGAGCAG 
GGACGATTGG TTTATAGATA CGGAGGTGAC TGTGTAGGGG CGTTCAAGCA AAAATCGGCT 
CGCCTGGCTC CAAAATCGAT TGCTCACGGC TTATTCTTGG ACCAATCTCA TGACAATCCG 
TCACCAATTC ACACTAGAAG CCCATTCGAC ATTCTTCCAA CCGCCGCCAT GCTCACAATG 
GCTTCTTGTG CTGTAGGTAG TAATCGAGGT TACGACGAGC TCATTAGAGA TCACATCCAC 
GTGGTTTCCG AAAAACGCCC ATATGCTTCT TGGTGCCGTC CGGATCAAGT GTCTAGAAGC 
CAGGGAATCA TTGAAGGCCG GAATTTGCTC AACAAGCTTC ATACGTGGCT CGCCGAGCAC 
GGATATTCTC AAGTTTTTGT GGATCAAATG AATTCGGATA TTGTGGGAAT CACGCGGCAC 
AATCCACGAA CCCACGAAAC GGTCGTAGTA GTGTCCCATA CTTCATTTTC CAAGAATTAT 
ATCGATTGGC CCGGCGGTTT GAAGCATATT CCCATTGGTG GAGTGCTGGA AAACGTGATT 
TTCGAGATGA AATTGAAAAA GGTTCAAGAG GAATGGGGAA CTGAAGATCC AGATGTTCTC 
ATAGGACTTA AAAATTATGA GATGGAAATT CGAGAAAATG TGAATTTGGA CAATGGAACT 
ATGTTCAAGG TTCATGATGG ATACATTGAG CTCACGAATT TCCCAACTGG ATCAGTTGTT 
GGATTTAAAA TTCGACCATC TGATGAAGCA ACTAAAGCTT TTAATATGAT TCATAACTCG 
ATTACTCCAG AACAATCTGA ATTTGACTCT GCTCTCTCTC GTCTCACATA TCAATCATTC 
CCAAATCTTC TATTCCACTG TGAATCCGAG GACTATGCCA CGATACAACA AGGCGGATAC 
GATGTTCCGA ATTTCGGAAA ATTCGTCTAC TGCGGCCTTC AGGGCCTCGT GCCAGTCCTC 
GAAAAAATCC GCGATGATAA CGACCTCGGG CATCCATTGT GCCAAAATCT CCGTGATGGG 
ACGTGGATCT GCGACTATAT TGTCGGAAGA TTGGCGAAAT TCGAGAAGCT CGGAGAAGTT 
TCAGAGGCTA TCAGAAAATT TTTTGCACCG TTGGATCATG TGCCTTATTA CCTCCGTCCG 
TGCTATTTTG AACTACTCGT TTCTTATATT TACGGAAAAA TTCGTAAAGA GGCGTTGAAG 
AGAATGGCTC CGCAAATCTC CTCGTCATCC GCGTTAGTCC GACACTTGGC CATCTCAACT 
CTCTCATTCC TTGGATACAT TCCTGGAGCC GGTCTCGCAC CGATTCCGAC TTCCCTCCAA 
ATTGAAGATC AGTATCCATC AAGTTTGGCC GCCGGGCTAT CCCACTTTGC TGTTGGAATC 
TGGAGAAATT GGGGTCGTGA CACATTTATC GCCTTGCCGG GATGCCTTTT ATCAACTGGC 
CGATTCCAGG AAGCCCGTCA AATCATTCTC TCATTTGCCG GCGCTATTCG TCATGGACTC 
ATACCGAATC TTCTAGCCGA GGGAATTGGT GCCAGATATA ATTGTAGAGA TGCCACGTGG 
TTCTGGCTGG TTTCGATTGT GAAATACGTG GAATCGGCGC CAAATGGAGT CGGGATTCTT 
GAGGATCCAG TGCGCAGAAT CTACCCGAAT GATGATAGTG TCTATGGAGA GGGAGAAGTG 
CAACAGATGC TTATTGAAAC TATTTATGAG GCACTTGACA AGCATTTCGC TGGGATTGAC 
TACAGGGAGA GAAGTGCCGG ACCACAAATC GACGAACAAA TGAGAGATGA GGGATTCCAG 
GTGACCGCCG GCGTCTCTCG CACCACAGGC TTCATCTACG GCGGTAATCG CTGGAATTGT 
GGAACATGGA TGGACAAGAT GGGAAGTAGT GAAAGAGCCG GAAATAAAGG AGAACCCGCG 
ACGCCGAGAG ATGGAGCGGC CGTCGAGCTA CAGGGACTTG CCTACAGAGC ACTGAAAAGC 
TTGAAGAATT GGAAGGAGCA AGGTGTGATC CAGAGATCCG GAGTTAGCGA CGAGTGGACG 
TGGGGCTTCT GGGCTCAGAA GATCCGAGAA AACTTTGAGA AGGAGTTCTT TGTTGATAAA 
GATTCCTATG CGGAGTTTGT AAATCGTCGG GAGATCATCA AGGATTCTGT CGGTTCCACA 
CTAGGATTCA CCGACTTCCA GCTTCGTTGC AACTTTGGAA TTGCATTAGC AGTTGCTCCA 
GATCTTATGG ATCCTAAGAA AGCATGGAAG GCATTGGATT CAGCTGAAGT TCTTCTTGGA 
CCACTTGGAA TGAAGACCCT TGATCCAACA GATTGGGCTT ACAACGGCTA CTACAACAAT 
GATGATGATG GGACTGACAA GAAGACGGCA AAAGGATGGA ATTATCATCA GGGCCCTGAA 
TGGCTCTTCG TCGCCGGGTA TTATCTCCAA GCTCGGCTGA GAATCGGTGA TATCCTCGGA 
GGATCCGAAA AGCAGTATGC GATCCGGCAA GTCCAGGAGC GACTCGGCAA TGCTTACAAG 
CACATCATTT CATCACCATG GAGGTCACTA CCGGAACTGA CAAATGCTGA TGGAGAATAC 
TGTATGCAGA GTTGTGCTGC CCAGGCGTGG TCCGTCGGTT GCCTGATTGA AGCATGTGTA 
AAACTTAATA CTATCGAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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agl-1 cDNA ORF clone, Caenorhabditis elegans

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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