hecd-1 cDNA ORF clone, Caenorhabditis elegans

  • Gene

  • Clones

  • gRNAs

The following hecd-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hecd-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa342642 NM_001306759.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342643 NM_001306760.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342644 NM_001306761.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342645 NM_001306762.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342646 NM_001306763.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342647 NM_001306764.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342648 NM_001306765.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342649 NM_001306766.2
Latest version!
Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
View Old Accession Versions >>
OCa342643 NM_001306760.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342645 NM_001306762.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342647 NM_001306764.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342642 NM_001306759.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342644 NM_001306761.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342646 NM_001306763.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342648 NM_001306765.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342649 NM_001306766.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa342642
    Clone ID Related Accession (Same CDS sequence) NM_001306759.1 , NM_001306759.2
    Accession Version NM_001306759.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293688.1
    CDS1..7947
    Translation

    Target ORF information:

    RefSeq Version NM_001306759.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342642
    Clone ID Related Accession (Same CDS sequence) NM_001306759.1 , NM_001306759.2
    Accession Version NM_001306759.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306759.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293688.1
    CDS107..8053
    Translation

    Target ORF information:

    RefSeq Version NM_001306759.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306759.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342643
    Clone ID Related Accession (Same CDS sequence) NM_001306760.1 , NM_001306760.2
    Accession Version NM_001306760.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293689.1
    CDS1..7836
    Translation

    Target ORF information:

    RefSeq Version NM_001306760.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342643
    Clone ID Related Accession (Same CDS sequence) NM_001306760.1 , NM_001306760.2
    Accession Version NM_001306760.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306760.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293689.1
    CDS107..7942
    Translation

    Target ORF information:

    RefSeq Version NM_001306760.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306760.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342644
    Clone ID Related Accession (Same CDS sequence) NM_001306761.1 , NM_001306761.2
    Accession Version NM_001306761.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293690.1
    CDS1..7824
    Translation

    Target ORF information:

    RefSeq Version NM_001306761.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342644
    Clone ID Related Accession (Same CDS sequence) NM_001306761.1 , NM_001306761.2
    Accession Version NM_001306761.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306761.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293690.1
    CDS242..8065
    Translation

    Target ORF information:

    RefSeq Version NM_001306761.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306761.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342645
    Clone ID Related Accession (Same CDS sequence) NM_001306762.2 , NM_001306762.1
    Accession Version NM_001306762.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306762.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293691.1
    CDS107..8041
    Translation

    Target ORF information:

    RefSeq Version NM_001306762.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306762.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342645
    Clone ID Related Accession (Same CDS sequence) NM_001306762.2 , NM_001306762.1
    Accession Version NM_001306762.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293691.1
    CDS1..7935
    Translation

    Target ORF information:

    RefSeq Version NM_001306762.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342646
    Clone ID Related Accession (Same CDS sequence) NM_001306763.1 , NM_001306763.2
    Accession Version NM_001306763.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293692.1
    CDS1..7941
    Translation

    Target ORF information:

    RefSeq Version NM_001306763.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342646
    Clone ID Related Accession (Same CDS sequence) NM_001306763.1 , NM_001306763.2
    Accession Version NM_001306763.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306763.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293692.1
    CDS242..8182
    Translation

    Target ORF information:

    RefSeq Version NM_001306763.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306763.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342647
    Clone ID Related Accession (Same CDS sequence) NM_001306764.2 , NM_001306764.1
    Accession Version NM_001306764.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306764.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293693.1
    CDS242..8194
    Translation

    Target ORF information:

    RefSeq Version NM_001306764.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306764.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342647
    Clone ID Related Accession (Same CDS sequence) NM_001306764.2 , NM_001306764.1
    Accession Version NM_001306764.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293693.1
    CDS1..7953
    Translation

    Target ORF information:

    RefSeq Version NM_001306764.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342648
    Clone ID Related Accession (Same CDS sequence) NM_001306765.1 , NM_001306765.2
    Accession Version NM_001306765.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293694.1
    CDS1..7842
    Translation

    Target ORF information:

    RefSeq Version NM_001306765.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342648
    Clone ID Related Accession (Same CDS sequence) NM_001306765.1 , NM_001306765.2
    Accession Version NM_001306765.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306765.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293694.1
    CDS242..8083
    Translation

    Target ORF information:

    RefSeq Version NM_001306765.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306765.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342649
    Clone ID Related Accession (Same CDS sequence) NM_001306766.1 , NM_001306766.2
    Accession Version NM_001306766.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293695.1
    CDS1..7830
    Translation

    Target ORF information:

    RefSeq Version NM_001306766.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342649
    Clone ID Related Accession (Same CDS sequence) NM_001306766.1 , NM_001306766.2
    Accession Version NM_001306766.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306766.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293695.1
    CDS242..8071
    Translation

    Target ORF information:

    RefSeq Version NM_001306766.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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