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spt-5 cDNA ORF clone, Caenorhabditis elegans

Summary

Gene Symbol spt-5
Entrez Gene ID 178143
Full Name Transcription elongation factor SPT5
Gene Type protein-coding
Organism Caenorhabditis elegans

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_069882.6 NP_502283.1 Transcription elongation factor SPT5
NM_069882.5 NP_502283.1 Transcription elongation factor SPT5

Pathways from BioSystems


Homologies


Anopheles gambiae (African malaria mosquito) AgaP_AGAP005021 XP_001231045.2
Caenorhabditis elegans (roundworm) spt-5 NP_502283.1
Xenopus tropicalis (tropical clawed frog) supt5h NP_001123796.1
Canis lupus familiaris (dog) SUPT5H XP_005616644.1
Mus musculus (house mouse) Supt5 NP_038704.1
Gallus gallus (chicken) SUPT5H NP_001026555.1
Neurospora crassa NCU06929 XP_959276.2
SUPT5H XP_001088495.1
Bos taurus (cattle) SUPT5H NP_001076908.2
Rattus norvegicus (Norway rat) Supt5h NP_001100967.2
Schizosaccharomyces pombe (fission yeast) spt5 NP_593191.1
Arabidopsis thaliana (thale cress) AT2G34210 NP_180968.2
Oryza sativa (rice) Os02g0772000 NP_001173159.1
Saccharomyces cerevisiae (baker's yeast) SPT5 NP_013703.1
Eremothecium gossypii AGOS_ADR187W NP_984283.2
Homo sapiens (human) SUPT5H NP_001104490.1
Pan troglodytes (chimpanzee) SUPT5H XP_512652.3
Danio rerio (zebrafish) supt5h NP_571748.2
Drosophila melanogaster (fruit fly) Spt5 NP_652610.1
Kluyveromyces lactis KLLA0B00891g XP_451570.1
Magnaporthe oryzae (rice blast fungus) MGG_12268 XP_003715018.1
Arabidopsis thaliana (thale cress) GTA2 NP_192575.3

Gene Ontology


Bibliography

Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spt-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spt-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa344413 NM_069882.6
Latest version!		 Caenorhabditis elegans Transcription elongation factor SPT5 (spt-5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OCa344413 NM_069882.5 Caenorhabditis elegans Transcription elongation factor SPT5 (spt-5), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCa344413
Clone ID Related Accession (Same CDS sequence) NM_069882.6 , NM_069882.5
Accession Version NM_069882.6 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-17
Organism Caenorhabditis elegans
Product Transcription elongation factor SPT5
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069882.5. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCCTCTG ACGAAAGTGA TGCAGCCTCA AACAAAGAAG AAGACACGAC TTTGAGCGAT 
GATGATGGCT CAGATGTTGA AATCAAAAAT TCAAAGAAGG ACAGAAAGCG GAAACTTGCT 
TCAGAGAGTG AGAAAAGTGG CTCGGACGAT GATGATGACG ACGATGACGA TGAAGATGAA 
AACGTGACCA GCAAGAAATC TAAAAAGCAG AAACGGAAGA ATAAGGCTCC TTCTGGACGA 
GATTTTCTTG CTTGGGATGT TGAAGTCGAC GACGAAGACG AAGACGACGA CAATGATTAC 
GATGACGAAG ACGATCCAAG CATGAATGCA ATGAACGAGC GAGAGGAAGC TGAGCGTGCG 
ATGAAAGAAA TGGAATTGAG TCAACGGAAT AGAGATCGGT ACAAGTTCCA AAACATGACG 
GAAGACGAAG TCCAGAAATA CTTTGAGAAC AAGTACAAGG GTGACAAAAA CGACAGTCAA 
TATGATGACG AAGATTCAGC TATGGATGAT ATTTCAAAAA ATTCTCATCT TCCATCCACG 
AAGGATCCTA ACCTGTGGAT TGTCAAATGT CGCATGGGAG AGGAAAAACT CGTAGCGATG 
CATCTGATGC GTAAATGTCT TGCGGTTGAG CACACAAACG AGCCATTCCA AATCAAATCA 
GTTGTCGTCA AGGAAGGACT CAAAGGAATG ATCTATATCG AGGCGTTCAA ACAATCTCAC 
GTCATGTCTG CAATTGAAGG ATTTTCAGCT CTCAATCAAT TCACTATAAC CATGGTTCCC 
ATCAAAGATA TGGTAGATGT ATTGAGAGTT GTCAAAGACA TTCCACAGTT GAAACTCGGA 
TCATATGTTC GTTTGAAAAG AACAATGTAC AAAGATGATC TGGCTGTCGT TGATCTTGTG 
GATATCGCTC AGAACCGTGT CAACCTGAAA TTGATTCCCC GTGTCGATTA TCAGAAGCGG 
CGCGGAGCAA TGAGAACTGA TGCTGATAAA AACTATAAGC TGAAAAGACG CCCAATGCCC 
AAGTTATTTG ATCAGGATAC CATCAAAGAA GTTGGAGGAG AAATTGTGAC GGACGGAGAC 
TTCTTAGTAT TTGAAGGAAA TCATTTCCGG CGAGGATTCC TCTACAAGTA CTTCCCCATA 
AACGCAATTC AAGCTGATGG TGTTAAACCA ACTCTTGGAG AACTCGAAAA GTTCCAAGAA 
TCTAGCGATG ATCTCAAGAG AGAATTGGAA ACGGCTTCTC TCAAAGACAC AGAAAATCCA 
TTTGTTCCTG GAGATATCGT CGAAGTGAAA GCTGGAGAGT TGGTGAATCT TCGGGGTAAA 
GTGATGACGG TCGATGGGAC GAAGGTTGTG ATGATGCCAG ATCAAGAAGA TTTGAAAGAA 
GCAATCACTT TGAACGCTCA TGAGCTCAGG AAATACTTCA AGGAAGGAGA TCATGCAAAG 
GTTATTAGTG GACGATACGA AGGACATACT GGATTAATTG TCCGAGTAAA AGATAGCACA 
GCCATAGTTT TGTCTGATCT TGGAATGGAA GAATTGAAAG TTCGTGTACG AGATTTGCAA 
CTTTGTGCTG ATGTGACAAC TGGAGTGGAC TCACTGGGAC AGTTCCAATA TCATGATCTT 
GTACAGCTCG ACCATACAGG AAATGTTGGA GTAATTGTTC GATTGGAGAA GGAACATTTG 
GAAGTTCTCA ACATGCACGG AGTTGTGAAT CGTATAAAGC CACAAGCTAT TATAGCGAAA 
AAAGACGTTC GATTTGCGAA AGTTCTCGAC AGTCAAAACA ATTCCATCGA GGCTAAAGAT 
CTGGTAAAAG TAATTGGAGG ACCAAACGCA AAGGAGCGGG AAACCGATGA GGATCCAGTT 
GGAGAAGTTC TCTATGCGTT CCGTGGAACT GTCTTCGTTT ATTCTCGCAA AGTGACTAAA 
AATGGAGGAG TCTTGGTTTG CAAACCAAAA CAACTGATTT TACAAGGAGC TAAGAAAACA 
ACCAGTACAC CGATGGTTTC TCGAATGGCT TCACCGAATC CTATGGCATC ACCAAGACAT 
TCTTCTGGAG GAATGACTCC TCGTTCTCCA CAAGATGGTA TGTCAAGTCG TGGAAGCAGT 
GGTGGACAAA CTCCGAGACA AGGTGGAGGA GGAGGACGTG GTGGTCATTT TGGAACTGCA 
AATCAACAAA AAGTTCGTCG TGATCTTACC CTCATTGGAA AAAATGTTCG AATCATCAAA 
GGTCCGATGA AGGGCCACTT TGGAATTGTT CGAGATGCCA CAGAAGACAC AGTTCGTGTC 
GAATTGCATA CACAATGCAG AACGATTTCT GTCGATCGTG CTCGTGTGAT GGTTGTCGGA 
GACACTGGAA TAACAGCTGG AAGTGGAGGT GGAAGCTCGT TCTACAGTTC TTCGAAGACA 
CCGATGCGCG ACTCTGGCAA AACTCCAATG TATGGATCGA AGACTCCGAT GTATGGAGCA 
CAAACACCGA TGTATGGCTC GATGACTCCA GCTTATGATG GAGGAAGAAC ACCGGCGTAT 
GGAGAAGGTG GACGTACCCC TGCATATGGA AGCAAAACTC CAGCATATGG AGATCTTGAT 
GAACATTCAT CATCACGTAC TCCAGCCTAT GGAAATGACA GTTCAAGAAC TCCTGCTTAC 
GGTAGTGCTG ATGGTGCACG AACTCCTGCC TATGGAAGTA CGGAAGGTGG ACGGACTCCA 
GCATACGGAT CAATGGACAA CAGTAGAACT CCTGCATATG ATGATTCTGG AAGAACGCCT 
GGCTATGAAA GTATGCCATC GAGAACTCCG AACTATGATT CAAGCAGTAA GACTCCAGCC 
TATCCAGAAT CCGAACATTC CGCCAGAACT CCAGCATACA ATAATGATTA TGATATTCCG 
TTGAGTCCGG CATACGAGCC CGATGCACCT GAAGCGTATG ACAATGCTCC AGCAAGAACT 
CCAGCATTCG TGTCCCGTAC TCCAGGATAC GACACATATG AGAACAGCAG CCCGACATAC 
GAACCAGATG CAGCTACAAA AGTTGAGGAA GACATCGGAG ACACTTCGTC TCCAACTTAT 
GATTCTCCTC CACACTCGTA CGTTGTGCCA ACTCCAGGTG CAATGCTCAA TCCAGCAACT 
CCGGGAGCCT ATCACGTTGA TACACCTGGA TTCGCTGCTC CAATGACGCC AGGAAGTGGA 
GGAGCATACG ATCAATATGT TGCGCCTTCT CCATTCGCTG GCTACGACAG TAACAACTAC 
AACAATGCCG ATGGAGCTAT TGAGCAAATT CCGGACCACT TCCTGGCTCA AGGTGTGTGG 
ATTGTGCAGA ATCTGTGTGT GCAAATCAAG GACCACGAAG GGCGATTCAA CGGACGTGAG 
GCGATCATTA AAGATGTTAC CGATGGAAAA GTGGATGTCT ACATGCCAGA TCACAAGTGC 
AACTTGGAAG TTGATTTCGA TCAGCTAACT CCAATGAAAC CGCAACCAGG AGATGATGCT 
CGAGTGATTT TCGGACAAGA TGCAGGTCAC AGTGGACAAC TTGTTTCCAT GGATGGATTC 
GAAGCAGTTA TCCGATCACA GGAAGACATG TCAGACATGC GAGTCATCAA CATTGGATTG 
TGCTGCAAAA TGCATAGCGA AACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_502283.1
CDS7..3633
Translation

Target ORF information:

RefSeq Version NM_069882.6
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans Transcription elongation factor SPT5 (spt-5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_069882.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCCTCTG ACGAAAGTGA TGCAGCCTCA AACAAAGAAG AAGACACGAC TTTGAGCGAT 
GATGATGGCT CAGATGTTGA AATCAAAAAT TCAAAGAAGG ACAGAAAGCG GAAACTTGCT 
TCAGAGAGTG AGAAAAGTGG CTCGGACGAT GATGATGACG ACGATGACGA TGAAGATGAA 
AACGTGACCA GCAAGAAATC TAAAAAGCAG AAACGGAAGA ATAAGGCTCC TTCTGGACGA 
GATTTTCTTG CTTGGGATGT TGAAGTCGAC GACGAAGACG AAGACGACGA CAATGATTAC 
GATGACGAAG ACGATCCAAG CATGAATGCA ATGAACGAGC GAGAGGAAGC TGAGCGTGCG 
ATGAAAGAAA TGGAATTGAG TCAACGGAAT AGAGATCGGT ACAAGTTCCA AAACATGACG 
GAAGACGAAG TCCAGAAATA CTTTGAGAAC AAGTACAAGG GTGACAAAAA CGACAGTCAA 
TATGATGACG AAGATTCAGC TATGGATGAT ATTTCAAAAA ATTCTCATCT TCCATCCACG 
AAGGATCCTA ACCTGTGGAT TGTCAAATGT CGCATGGGAG AGGAAAAACT CGTAGCGATG 
CATCTGATGC GTAAATGTCT TGCGGTTGAG CACACAAACG AGCCATTCCA AATCAAATCA 
GTTGTCGTCA AGGAAGGACT CAAAGGAATG ATCTATATCG AGGCGTTCAA ACAATCTCAC 
GTCATGTCTG CAATTGAAGG ATTTTCAGCT CTCAATCAAT TCACTATAAC CATGGTTCCC 
ATCAAAGATA TGGTAGATGT ATTGAGAGTT GTCAAAGACA TTCCACAGTT GAAACTCGGA 
TCATATGTTC GTTTGAAAAG AACAATGTAC AAAGATGATC TGGCTGTCGT TGATCTTGTG 
GATATCGCTC AGAACCGTGT CAACCTGAAA TTGATTCCCC GTGTCGATTA TCAGAAGCGG 
CGCGGAGCAA TGAGAACTGA TGCTGATAAA AACTATAAGC TGAAAAGACG CCCAATGCCC 
AAGTTATTTG ATCAGGATAC CATCAAAGAA GTTGGAGGAG AAATTGTGAC GGACGGAGAC 
TTCTTAGTAT TTGAAGGAAA TCATTTCCGG CGAGGATTCC TCTACAAGTA CTTCCCCATA 
AACGCAATTC AAGCTGATGG TGTTAAACCA ACTCTTGGAG AACTCGAAAA GTTCCAAGAA 
TCTAGCGATG ATCTCAAGAG AGAATTGGAA ACGGCTTCTC TCAAAGACAC AGAAAATCCA 
TTTGTTCCTG GAGATATCGT CGAAGTGAAA GCTGGAGAGT TGGTGAATCT TCGGGGTAAA 
GTGATGACGG TCGATGGGAC GAAGGTTGTG ATGATGCCAG ATCAAGAAGA TTTGAAAGAA 
GCAATCACTT TGAACGCTCA TGAGCTCAGG AAATACTTCA AGGAAGGAGA TCATGCAAAG 
GTTATTAGTG GACGATACGA AGGACATACT GGATTAATTG TCCGAGTAAA AGATAGCACA 
GCCATAGTTT TGTCTGATCT TGGAATGGAA GAATTGAAAG TTCGTGTACG AGATTTGCAA 
CTTTGTGCTG ATGTGACAAC TGGAGTGGAC TCACTGGGAC AGTTCCAATA TCATGATCTT 
GTACAGCTCG ACCATACAGG AAATGTTGGA GTAATTGTTC GATTGGAGAA GGAACATTTG 
GAAGTTCTCA ACATGCACGG AGTTGTGAAT CGTATAAAGC CACAAGCTAT TATAGCGAAA 
AAAGACGTTC GATTTGCGAA AGTTCTCGAC AGTCAAAACA ATTCCATCGA GGCTAAAGAT 
CTGGTAAAAG TAATTGGAGG ACCAAACGCA AAGGAGCGGG AAACCGATGA GGATCCAGTT 
GGAGAAGTTC TCTATGCGTT CCGTGGAACT GTCTTCGTTT ATTCTCGCAA AGTGACTAAA 
AATGGAGGAG TCTTGGTTTG CAAACCAAAA CAACTGATTT TACAAGGAGC TAAGAAAACA 
ACCAGTACAC CGATGGTTTC TCGAATGGCT TCACCGAATC CTATGGCATC ACCAAGACAT 
TCTTCTGGAG GAATGACTCC TCGTTCTCCA CAAGATGGTA TGTCAAGTCG TGGAAGCAGT 
GGTGGACAAA CTCCGAGACA AGGTGGAGGA GGAGGACGTG GTGGTCATTT TGGAACTGCA 
AATCAACAAA AAGTTCGTCG TGATCTTACC CTCATTGGAA AAAATGTTCG AATCATCAAA 
GGTCCGATGA AGGGCCACTT TGGAATTGTT CGAGATGCCA CAGAAGACAC AGTTCGTGTC 
GAATTGCATA CACAATGCAG AACGATTTCT GTCGATCGTG CTCGTGTGAT GGTTGTCGGA 
GACACTGGAA TAACAGCTGG AAGTGGAGGT GGAAGCTCGT TCTACAGTTC TTCGAAGACA 
CCGATGCGCG ACTCTGGCAA AACTCCAATG TATGGATCGA AGACTCCGAT GTATGGAGCA 
CAAACACCGA TGTATGGCTC GATGACTCCA GCTTATGATG GAGGAAGAAC ACCGGCGTAT 
GGAGAAGGTG GACGTACCCC TGCATATGGA AGCAAAACTC CAGCATATGG AGATCTTGAT 
GAACATTCAT CATCACGTAC TCCAGCCTAT GGAAATGACA GTTCAAGAAC TCCTGCTTAC 
GGTAGTGCTG ATGGTGCACG AACTCCTGCC TATGGAAGTA CGGAAGGTGG ACGGACTCCA 
GCATACGGAT CAATGGACAA CAGTAGAACT CCTGCATATG ATGATTCTGG AAGAACGCCT 
GGCTATGAAA GTATGCCATC GAGAACTCCG AACTATGATT CAAGCAGTAA GACTCCAGCC 
TATCCAGAAT CCGAACATTC CGCCAGAACT CCAGCATACA ATAATGATTA TGATATTCCG 
TTGAGTCCGG CATACGAGCC CGATGCACCT GAAGCGTATG ACAATGCTCC AGCAAGAACT 
CCAGCATTCG TGTCCCGTAC TCCAGGATAC GACACATATG AGAACAGCAG CCCGACATAC 
GAACCAGATG CAGCTACAAA AGTTGAGGAA GACATCGGAG ACACTTCGTC TCCAACTTAT 
GATTCTCCTC CACACTCGTA CGTTGTGCCA ACTCCAGGTG CAATGCTCAA TCCAGCAACT 
CCGGGAGCCT ATCACGTTGA TACACCTGGA TTCGCTGCTC CAATGACGCC AGGAAGTGGA 
GGAGCATACG ATCAATATGT TGCGCCTTCT CCATTCGCTG GCTACGACAG TAACAACTAC 
AACAATGCCG ATGGAGCTAT TGAGCAAATT CCGGACCACT TCCTGGCTCA AGGTGTGTGG 
ATTGTGCAGA ATCTGTGTGT GCAAATCAAG GACCACGAAG GGCGATTCAA CGGACGTGAG 
GCGATCATTA AAGATGTTAC CGATGGAAAA GTGGATGTCT ACATGCCAGA TCACAAGTGC 
AACTTGGAAG TTGATTTCGA TCAGCTAACT CCAATGAAAC CGCAACCAGG AGATGATGCT 
CGAGTGATTT TCGGACAAGA TGCAGGTCAC AGTGGACAAC TTGTTTCCAT GGATGGATTC 
GAAGCAGTTA TCCGATCACA GGAAGACATG TCAGACATGC GAGTCATCAA CATTGGATTG 
TGCTGCAAAA TGCATAGCGA AACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa344413
Clone ID Related Accession (Same CDS sequence) NM_069882.6 , NM_069882.5
Accession Version NM_069882.5 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-08
Organism Caenorhabditis elegans
Product Transcription elongation factor SPT5
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069882.4. COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCCTCTG ACGAAAGTGA TGCAGCCTCA AACAAAGAAG AAGACACGAC TTTGAGCGAT 
GATGATGGCT CAGATGTTGA AATCAAAAAT TCAAAGAAGG ACAGAAAGCG GAAACTTGCT 
TCAGAGAGTG AGAAAAGTGG CTCGGACGAT GATGATGACG ACGATGACGA TGAAGATGAA 
AACGTGACCA GCAAGAAATC TAAAAAGCAG AAACGGAAGA ATAAGGCTCC TTCTGGACGA 
GATTTTCTTG CTTGGGATGT TGAAGTCGAC GACGAAGACG AAGACGACGA CAATGATTAC 
GATGACGAAG ACGATCCAAG CATGAATGCA ATGAACGAGC GAGAGGAAGC TGAGCGTGCG 
ATGAAAGAAA TGGAATTGAG TCAACGGAAT AGAGATCGGT ACAAGTTCCA AAACATGACG 
GAAGACGAAG TCCAGAAATA CTTTGAGAAC AAGTACAAGG GTGACAAAAA CGACAGTCAA 
TATGATGACG AAGATTCAGC TATGGATGAT ATTTCAAAAA ATTCTCATCT TCCATCCACG 
AAGGATCCTA ACCTGTGGAT TGTCAAATGT CGCATGGGAG AGGAAAAACT CGTAGCGATG 
CATCTGATGC GTAAATGTCT TGCGGTTGAG CACACAAACG AGCCATTCCA AATCAAATCA 
GTTGTCGTCA AGGAAGGACT CAAAGGAATG ATCTATATCG AGGCGTTCAA ACAATCTCAC 
GTCATGTCTG CAATTGAAGG ATTTTCAGCT CTCAATCAAT TCACTATAAC CATGGTTCCC 
ATCAAAGATA TGGTAGATGT ATTGAGAGTT GTCAAAGACA TTCCACAGTT GAAACTCGGA 
TCATATGTTC GTTTGAAAAG AACAATGTAC AAAGATGATC TGGCTGTCGT TGATCTTGTG 
GATATCGCTC AGAACCGTGT CAACCTGAAA TTGATTCCCC GTGTCGATTA TCAGAAGCGG 
CGCGGAGCAA TGAGAACTGA TGCTGATAAA AACTATAAGC TGAAAAGACG CCCAATGCCC 
AAGTTATTTG ATCAGGATAC CATCAAAGAA GTTGGAGGAG AAATTGTGAC GGACGGAGAC 
TTCTTAGTAT TTGAAGGAAA TCATTTCCGG CGAGGATTCC TCTACAAGTA CTTCCCCATA 
AACGCAATTC AAGCTGATGG TGTTAAACCA ACTCTTGGAG AACTCGAAAA GTTCCAAGAA 
TCTAGCGATG ATCTCAAGAG AGAATTGGAA ACGGCTTCTC TCAAAGACAC AGAAAATCCA 
TTTGTTCCTG GAGATATCGT CGAAGTGAAA GCTGGAGAGT TGGTGAATCT TCGGGGTAAA 
GTGATGACGG TCGATGGGAC GAAGGTTGTG ATGATGCCAG ATCAAGAAGA TTTGAAAGAA 
GCAATCACTT TGAACGCTCA TGAGCTCAGG AAATACTTCA AGGAAGGAGA TCATGCAAAG 
GTTATTAGTG GACGATACGA AGGACATACT GGATTAATTG TCCGAGTAAA AGATAGCACA 
GCCATAGTTT TGTCTGATCT TGGAATGGAA GAATTGAAAG TTCGTGTACG AGATTTGCAA 
CTTTGTGCTG ATGTGACAAC TGGAGTGGAC TCACTGGGAC AGTTCCAATA TCATGATCTT 
GTACAGCTCG ACCATACAGG AAATGTTGGA GTAATTGTTC GATTGGAGAA GGAACATTTG 
GAAGTTCTCA ACATGCACGG AGTTGTGAAT CGTATAAAGC CACAAGCTAT TATAGCGAAA 
AAAGACGTTC GATTTGCGAA AGTTCTCGAC AGTCAAAACA ATTCCATCGA GGCTAAAGAT 
CTGGTAAAAG TAATTGGAGG ACCAAACGCA AAGGAGCGGG AAACCGATGA GGATCCAGTT 
GGAGAAGTTC TCTATGCGTT CCGTGGAACT GTCTTCGTTT ATTCTCGCAA AGTGACTAAA 
AATGGAGGAG TCTTGGTTTG CAAACCAAAA CAACTGATTT TACAAGGAGC TAAGAAAACA 
ACCAGTACAC CGATGGTTTC TCGAATGGCT TCACCGAATC CTATGGCATC ACCAAGACAT 
TCTTCTGGAG GAATGACTCC TCGTTCTCCA CAAGATGGTA TGTCAAGTCG TGGAAGCAGT 
GGTGGACAAA CTCCGAGACA AGGTGGAGGA GGAGGACGTG GTGGTCATTT TGGAACTGCA 
AATCAACAAA AAGTTCGTCG TGATCTTACC CTCATTGGAA AAAATGTTCG AATCATCAAA 
GGTCCGATGA AGGGCCACTT TGGAATTGTT CGAGATGCCA CAGAAGACAC AGTTCGTGTC 
GAATTGCATA CACAATGCAG AACGATTTCT GTCGATCGTG CTCGTGTGAT GGTTGTCGGA 
GACACTGGAA TAACAGCTGG AAGTGGAGGT GGAAGCTCGT TCTACAGTTC TTCGAAGACA 
CCGATGCGCG ACTCTGGCAA AACTCCAATG TATGGATCGA AGACTCCGAT GTATGGAGCA 
CAAACACCGA TGTATGGCTC GATGACTCCA GCTTATGATG GAGGAAGAAC ACCGGCGTAT 
GGAGAAGGTG GACGTACCCC TGCATATGGA AGCAAAACTC CAGCATATGG AGATCTTGAT 
GAACATTCAT CATCACGTAC TCCAGCCTAT GGAAATGACA GTTCAAGAAC TCCTGCTTAC 
GGTAGTGCTG ATGGTGCACG AACTCCTGCC TATGGAAGTA CGGAAGGTGG ACGGACTCCA 
GCATACGGAT CAATGGACAA CAGTAGAACT CCTGCATATG ATGATTCTGG AAGAACGCCT 
GGCTATGAAA GTATGCCATC GAGAACTCCG AACTATGATT CAAGCAGTAA GACTCCAGCC 
TATCCAGAAT CCGAACATTC CGCCAGAACT CCAGCATACA ATAATGATTA TGATATTCCG 
TTGAGTCCGG CATACGAGCC CGATGCACCT GAAGCGTATG ACAATGCTCC AGCAAGAACT 
CCAGCATTCG TGTCCCGTAC TCCAGGATAC GACACATATG AGAACAGCAG CCCGACATAC 
GAACCAGATG CAGCTACAAA AGTTGAGGAA GACATCGGAG ACACTTCGTC TCCAACTTAT 
GATTCTCCTC CACACTCGTA CGTTGTGCCA ACTCCAGGTG CAATGCTCAA TCCAGCAACT 
CCGGGAGCCT ATCACGTTGA TACACCTGGA TTCGCTGCTC CAATGACGCC AGGAAGTGGA 
GGAGCATACG ATCAATATGT TGCGCCTTCT CCATTCGCTG GCTACGACAG TAACAACTAC 
AACAATGCCG ATGGAGCTAT TGAGCAAATT CCGGACCACT TCCTGGCTCA AGGTGTGTGG 
ATTGTGCAGA ATCTGTGTGT GCAAATCAAG GACCACGAAG GGCGATTCAA CGGACGTGAG 
GCGATCATTA AAGATGTTAC CGATGGAAAA GTGGATGTCT ACATGCCAGA TCACAAGTGC 
AACTTGGAAG TTGATTTCGA TCAGCTAACT CCAATGAAAC CGCAACCAGG AGATGATGCT 
CGAGTGATTT TCGGACAAGA TGCAGGTCAC AGTGGACAAC TTGTTTCCAT GGATGGATTC 
GAAGCAGTTA TCCGATCACA GGAAGACATG TCAGACATGC GAGTCATCAA CATTGGATTG 
TGCTGCAAAA TGCATAGCGA AACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_502283.1
CDS1..3627
Translation

Target ORF information:

RefSeq Version NM_069882.5
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans Transcription elongation factor SPT5 (spt-5), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_069882.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGTCCTCTG ACGAAAGTGA TGCAGCCTCA AACAAAGAAG AAGACACGAC TTTGAGCGAT 
GATGATGGCT CAGATGTTGA AATCAAAAAT TCAAAGAAGG ACAGAAAGCG GAAACTTGCT 
TCAGAGAGTG AGAAAAGTGG CTCGGACGAT GATGATGACG ACGATGACGA TGAAGATGAA 
AACGTGACCA GCAAGAAATC TAAAAAGCAG AAACGGAAGA ATAAGGCTCC TTCTGGACGA 
GATTTTCTTG CTTGGGATGT TGAAGTCGAC GACGAAGACG AAGACGACGA CAATGATTAC 
GATGACGAAG ACGATCCAAG CATGAATGCA ATGAACGAGC GAGAGGAAGC TGAGCGTGCG 
ATGAAAGAAA TGGAATTGAG TCAACGGAAT AGAGATCGGT ACAAGTTCCA AAACATGACG 
GAAGACGAAG TCCAGAAATA CTTTGAGAAC AAGTACAAGG GTGACAAAAA CGACAGTCAA 
TATGATGACG AAGATTCAGC TATGGATGAT ATTTCAAAAA ATTCTCATCT TCCATCCACG 
AAGGATCCTA ACCTGTGGAT TGTCAAATGT CGCATGGGAG AGGAAAAACT CGTAGCGATG 
CATCTGATGC GTAAATGTCT TGCGGTTGAG CACACAAACG AGCCATTCCA AATCAAATCA 
GTTGTCGTCA AGGAAGGACT CAAAGGAATG ATCTATATCG AGGCGTTCAA ACAATCTCAC 
GTCATGTCTG CAATTGAAGG ATTTTCAGCT CTCAATCAAT TCACTATAAC CATGGTTCCC 
ATCAAAGATA TGGTAGATGT ATTGAGAGTT GTCAAAGACA TTCCACAGTT GAAACTCGGA 
TCATATGTTC GTTTGAAAAG AACAATGTAC AAAGATGATC TGGCTGTCGT TGATCTTGTG 
GATATCGCTC AGAACCGTGT CAACCTGAAA TTGATTCCCC GTGTCGATTA TCAGAAGCGG 
CGCGGAGCAA TGAGAACTGA TGCTGATAAA AACTATAAGC TGAAAAGACG CCCAATGCCC 
AAGTTATTTG ATCAGGATAC CATCAAAGAA GTTGGAGGAG AAATTGTGAC GGACGGAGAC 
TTCTTAGTAT TTGAAGGAAA TCATTTCCGG CGAGGATTCC TCTACAAGTA CTTCCCCATA 
AACGCAATTC AAGCTGATGG TGTTAAACCA ACTCTTGGAG AACTCGAAAA GTTCCAAGAA 
TCTAGCGATG ATCTCAAGAG AGAATTGGAA ACGGCTTCTC TCAAAGACAC AGAAAATCCA 
TTTGTTCCTG GAGATATCGT CGAAGTGAAA GCTGGAGAGT TGGTGAATCT TCGGGGTAAA 
GTGATGACGG TCGATGGGAC GAAGGTTGTG ATGATGCCAG ATCAAGAAGA TTTGAAAGAA 
GCAATCACTT TGAACGCTCA TGAGCTCAGG AAATACTTCA AGGAAGGAGA TCATGCAAAG 
GTTATTAGTG GACGATACGA AGGACATACT GGATTAATTG TCCGAGTAAA AGATAGCACA 
GCCATAGTTT TGTCTGATCT TGGAATGGAA GAATTGAAAG TTCGTGTACG AGATTTGCAA 
CTTTGTGCTG ATGTGACAAC TGGAGTGGAC TCACTGGGAC AGTTCCAATA TCATGATCTT 
GTACAGCTCG ACCATACAGG AAATGTTGGA GTAATTGTTC GATTGGAGAA GGAACATTTG 
GAAGTTCTCA ACATGCACGG AGTTGTGAAT CGTATAAAGC CACAAGCTAT TATAGCGAAA 
AAAGACGTTC GATTTGCGAA AGTTCTCGAC AGTCAAAACA ATTCCATCGA GGCTAAAGAT 
CTGGTAAAAG TAATTGGAGG ACCAAACGCA AAGGAGCGGG AAACCGATGA GGATCCAGTT 
GGAGAAGTTC TCTATGCGTT CCGTGGAACT GTCTTCGTTT ATTCTCGCAA AGTGACTAAA 
AATGGAGGAG TCTTGGTTTG CAAACCAAAA CAACTGATTT TACAAGGAGC TAAGAAAACA 
ACCAGTACAC CGATGGTTTC TCGAATGGCT TCACCGAATC CTATGGCATC ACCAAGACAT 
TCTTCTGGAG GAATGACTCC TCGTTCTCCA CAAGATGGTA TGTCAAGTCG TGGAAGCAGT 
GGTGGACAAA CTCCGAGACA AGGTGGAGGA GGAGGACGTG GTGGTCATTT TGGAACTGCA 
AATCAACAAA AAGTTCGTCG TGATCTTACC CTCATTGGAA AAAATGTTCG AATCATCAAA 
GGTCCGATGA AGGGCCACTT TGGAATTGTT CGAGATGCCA CAGAAGACAC AGTTCGTGTC 
GAATTGCATA CACAATGCAG AACGATTTCT GTCGATCGTG CTCGTGTGAT GGTTGTCGGA 
GACACTGGAA TAACAGCTGG AAGTGGAGGT GGAAGCTCGT TCTACAGTTC TTCGAAGACA 
CCGATGCGCG ACTCTGGCAA AACTCCAATG TATGGATCGA AGACTCCGAT GTATGGAGCA 
CAAACACCGA TGTATGGCTC GATGACTCCA GCTTATGATG GAGGAAGAAC ACCGGCGTAT 
GGAGAAGGTG GACGTACCCC TGCATATGGA AGCAAAACTC CAGCATATGG AGATCTTGAT 
GAACATTCAT CATCACGTAC TCCAGCCTAT GGAAATGACA GTTCAAGAAC TCCTGCTTAC 
GGTAGTGCTG ATGGTGCACG AACTCCTGCC TATGGAAGTA CGGAAGGTGG ACGGACTCCA 
GCATACGGAT CAATGGACAA CAGTAGAACT CCTGCATATG ATGATTCTGG AAGAACGCCT 
GGCTATGAAA GTATGCCATC GAGAACTCCG AACTATGATT CAAGCAGTAA GACTCCAGCC 
TATCCAGAAT CCGAACATTC CGCCAGAACT CCAGCATACA ATAATGATTA TGATATTCCG 
TTGAGTCCGG CATACGAGCC CGATGCACCT GAAGCGTATG ACAATGCTCC AGCAAGAACT 
CCAGCATTCG TGTCCCGTAC TCCAGGATAC GACACATATG AGAACAGCAG CCCGACATAC 
GAACCAGATG CAGCTACAAA AGTTGAGGAA GACATCGGAG ACACTTCGTC TCCAACTTAT 
GATTCTCCTC CACACTCGTA CGTTGTGCCA ACTCCAGGTG CAATGCTCAA TCCAGCAACT 
CCGGGAGCCT ATCACGTTGA TACACCTGGA TTCGCTGCTC CAATGACGCC AGGAAGTGGA 
GGAGCATACG ATCAATATGT TGCGCCTTCT CCATTCGCTG GCTACGACAG TAACAACTAC 
AACAATGCCG ATGGAGCTAT TGAGCAAATT CCGGACCACT TCCTGGCTCA AGGTGTGTGG 
ATTGTGCAGA ATCTGTGTGT GCAAATCAAG GACCACGAAG GGCGATTCAA CGGACGTGAG 
GCGATCATTA AAGATGTTAC CGATGGAAAA GTGGATGTCT ACATGCCAGA TCACAAGTGC 
AACTTGGAAG TTGATTTCGA TCAGCTAACT CCAATGAAAC CGCAACCAGG AGATGATGCT 
CGAGTGATTT TCGGACAAGA TGCAGGTCAC AGTGGACAAC TTGTTTCCAT GGATGGATTC 
GAAGCAGTTA TCCGATCACA GGAAGACATG TCAGACATGC GAGTCATCAA CATTGGATTG 
TGCTGCAAAA TGCATAGCGA AACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)